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Metabolomic analysis-identified 2-hydroxybutyric acid might be a key metabolite of severe preeclampsia

  • Fang Wang , Lili Xu , Mingming Qi , Huimin Lai , Fanhua Zeng , Furong Liang , Qing Wen , Xihua Ma , Chan Zhang and Kaili Xie EMAIL logo
Published/Copyright: February 28, 2023
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Open Life Sciences
From the journal Open Life Sciences Volume 18 Issue 1

Abstract

This study set out to determine the key metabolite changes underlying the pathophysiology of severe preeclampsia (PE) using metabolic analysis. We collected sera from 10 patients with severe PE and from 10 healthy pregnant women of the same trimester and analyzed them using liquid chromatography mass spectrometry. A total of 3,138 differential metabolites were screened, resulting in the identification of 124 differential metabolites. Kyoto encyclopedia of genes and genomes pathway analysis revealed that they were mainly enriched in the following metabolic pathways: central carbon metabolism in cancer; protein digestion and absorption; aminoacyl-transfer RNA biosynthesis; mineral absorption; alanine, aspartate, and glutamate metabolism; and prostate cancer. After analysis of 124 differential metabolites, 2-hydroxybutyric acid was found to be the most critical differential metabolite, and its use allowed the differentiation of women with severe PE from healthy pregnant women. In summary, our analysis revealed that 2-hydroxybutyric acid is a potential key metabolite for distinguishing severe PE from healthy controls and is also a marker for the early diagnosis of severe PE, thus allowing early intervention.

Keywords: severe preeclampsia; liquid chromatograph mass spectrometer; metabolomic analysis; 2-hydroxybutyric acid

1 Introduction

Preeclampsia (PE) is defined as a new onset of hypertension during pregnancy after the 20th week [1], and its global prevalence is 8% [2]. Worldwide, PE and eclampsia are major causes of maternal and infant mortality [3]. In developing countries, it occurs at a rate of 1.8–16.7% [4] and causes 40–60% of maternal deaths [4]. Its rates have remained unchanged for decades, but the rates of severe PE have increased over recent decades [5]. Eclampsia can occur in patients with severe PE leading to symptoms of the nervous system [6] or hemolysis, elevated liver enzymes, low platelet count (HELLP) syndrome [7]. Depending on the clinical characteristics of a patient, PE can be classified as mild or severe [8]. Furthermore, it can be classified according to its time of clinical manifestation as “early-onset PE” (EOPE) in cases occurring before 34 weeks of pregnancy, or as “late-onset PE” (LOPE) in cases occurring after 34 weeks of pregnancy [9]. EOPE and LOPE might be more useful subclassifications [10]. The majority of the affected women suffers from PE at the late preterm or term stage, but about 12% suffers from PE that begins early (before 34 weeks of pregnancy) [11]. EOPE is the result of placental defects and deficiency of trophoblast invasion and normal spiral artery remodeling; LOPE, however, may result from interactions between the normal senescence of the placenta and a maternal genetic history of cardiovascular disease [12,13]. Blood pressure (BP) control is crucial during PE to prevent systemic complications [14]. Therefore, early diagnosis and early intervention in PE are particularly important.

There are many established risk factors for PE, such as nulliparity, advanced maternal age, overweight or obesity, chronic hypertension, diabetes, previous PE, family history of PE, and multiple pregnancy [15]; however, the exact causes of PE/eclampsia remain unclear [16]. Further research is needed concerning the cellular and molecular mechanisms of PE to improve the treatment of PE patients [17].

The study of metabonomics and metabolomics involves the use of accurate metabonomic (and/or metabolomic) analyses of metabolic changes occurring in cells, tissues, and whole organisms [18]. It is part of the “omics cascade” together with genomics, transcriptomics, and proteomics [19] and one of the many “-omics” technologies that are currently being developed [20]. Metabolomics, or metabonomics, primarily involves the elucidation of the end products in a specific organism or a cell [21], and it is the “ultimate” tool in the “omics chain,” as it is the closest to the phenotype [22]. It can be divided into two categories: untargeted and targeted [23]. While the former also known as discovery metabolomics, which is a global analysis of different metabolomics between control and experimental groups, the latter focuses on the analysis of specific metabolic clusters associated with certain metabolic pathways [20].

2-Hydroxybutyric acid is elevated in many diseases and has some diagnostic values. In cancer, its levels have been elevated in a mouse model of colon carcinogenesis induced by azoxymethane/dextran sodium sulfate [24]; furthermore, nuclear lactate dehydrogenase A induces its production from reactive oxygen species and promotes human papilloma virus-induced cervical tumor growth [25]; moreover, patients in the initial diagnostic stage of acute myeloid leukemia can be identified by 2-hydroxybutyric acid [26]. In pneumonia diseases, compared to healthy controls, 2-hydroxybutyric acid was found to be enriched in COVID-19 patients and COVID-like patients and remained at higher levels after discharge [27]; meanwhile, elevated dehydrogenase can be an independent prognostic factor for death in hospitalized COVID-19 patients [28]; it also has a diagnostic value in community-acquired pneumonia [29]. 2-Hydroxybutyric acid has been more deeply studied in diabetes than in any other disease, and high levels of plasma are a good predictor of type 2 diabetes [30]. Furthermore, it can be used in the following circumstances: as a biomarker of insulin resistance; for disease tracking throughout the treatment of insulin resistance [31]; as a predictive marker for impaired glucose tolerance without the need for a glucose tolerance test [32]; along with branched-chain amino acids, it can predict worsening glycemic control in adolescents [33]; it has high values in the sera of patients with isolated postchallenge diabetes compared to normal subjects [34]; and its levels decrease significantly after laparoscopic sleeve gastrectomy in morbidly obese patients [35]. It also have some diagnostic values in other diseases: it is significantly higher in the blood of pregnant women carrying trisomy 21 fetuses than in healthy pregnant women [36]; urinary 2-hydroxybutyric acid predicts the development of acute kidney injury in presurgical samples [37]; and its levels are elevated in patients with a major depressive disorder [38].

In this study, we collected sera from 10 patients with severe PE and 10 healthy pregnant women of the same trimester and analyzed them using liquid chromatograph mass spectrometry (LC-MS), with a view to identifying the key metabolites of the former pathogenesis and providing new indicators for early diagnosis.

2 Materials and methods

2.1 Study population

All samples were obtained from the Obstetrics Department of Zhuzhou Central Hospital and were divided into a normal control group (10 normotensive pregnant women) and a severe PE group (n = 10). The basic diagnostic criteria for PE are as follows: BP ≥140/90 mmHg, and urine protein ≥0.3 mg/24 h. Severe PE is diagnosed on the basis of the diagnostic criteria for PE with any of the following conditions present: systolic BP ≥160 mmHg, or diastolic BP ≥110 mmHg, or other manifestations of a multisystem disorder (e.g., severe proteinuria, thrombocytopenia, impaired liver function, severe persistent right upper quadrant or epigastric pain, renal insufficiency, pulmonary edema, or new-onset headache).

  1. Informed consent: Informed consent has been obtained from all individuals included in this study.

  2. Ethical approval: The research related to human use has been complied with all the relevant national regulations, institutional policies and in accordance with the tenets of the Helsinki Declaration, and has been approved by the Institutional Research Ethics Board of Zhuzhou Central Hospital (reference number 20180334).

2.2 Sample processing

A peripheral blood sample (10 mL) was taken from each participant. All samples were centrifuged at 2,000 rpm for 10 min at room temperature using a centrifuge. Afterward, the supernatant was stored in a refrigerator at −80°C.

2.3 Spectroscopy

All samples were thawed at 4°C (insufficient samples were reduced to an equal scale); 100 µL of each sample was transferred into 2 mL centrifuge tubes (samples with a sample size of <50 µL were extracted by half of the experimental system, but the resolution system remained unchanged); 400 µL of methanol (−20°C) was added to each tube and vortexed for 60 s; the mixture was centrifuged at 4°C for 10 min at 12,000 rpm, and then the supernatant was transferred from each sample into another 2 mL centrifuge tube. Samples were concentrated to dry in a vacuum and subsequently dissolved with 150 µL 2-chlorobenzalanine (4 ppm) 80% methanol solution, and the supernatant was filtered through a 0.22 µm membrane to obtain the prepared samples for gas chromatography mass spectrometry (GC-MS). For quality control (QC), 20 µL subsamples were taken (QC samples were used to monitor deviations of the analytical results from these pool mixtures and compare them to the errors caused by the analytical instrument itself). The remainder of the samples were used for LC-MS detection

2.4 Chromatography and mass spectrometry conditions

Chromatographic separation was performed with an ACQUITY UPLC® HSS T3 (150 mm × 2.1 mm, 1.8 µm, Waters) column maintained at 40°C. The temperature of the autosampler was 8°C. Gradient elution of analytes was carried out with 0.1% formic acid in water and 0.1% formic acid in acetonitrile or 5 mM ammonium formate in water and acetonitrile at a flow rate of 0.25 mL/min. Injection of 2 μL of each sample was performed after equilibration. An increasing linear gradient of solvent B (v/v) was used as follows: 0–1 min, 2% B/D; 1–9 min, 2–50% B/D; 9–12 min, 50–98% B/D; 12–13.5 min, 98% B/D; 13.5–14 min, 98–2% B/D; 14–20 min, 2% D-positive model (14–17 min, 2% B-negative model).

The electrospray ionization multistage mass spectrometry experiments were used with a spray voltage of 3.5 and −2.5 kV in positive and negative modes, respectively. Sheath gas and auxiliary gas were set at 30 and 10 arbitrary units, respectively, while the capillary temperature was 325°C. The Orbitrap analyzer scanned over a mass range of m/z 81–1,000 for a full scan at a mass resolution of 70,000. Data-dependent acquisition MS/MS experiments were performed with a higher energy collisional dissociation scan. The normalized collision energy was 30 eV. Dynamic exclusion was implemented to remove some unnecessary information in MS/MS spectra.

2.5 Multivariate statistical analysis

The data were analyzed using SIMCA-P (v13.0) [35] software and the R language ropls [39] package. The main methods of analysis included principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) [40].

Unsupervised analysis (e.g., PCA) does not ignore within-group errors, eliminates random errors that are not relevant to the purpose of the study, focuses too much on details, and neglects the overall picture and patterns, and is ultimately detrimental to the detection of between-group differences and differential compounds. In such cases, it is necessary to use prior knowledge of the sample to further focus the data analysis on the aspect being studied, using a supervised analysis such as PLS-DA. OPLS-DA, another commonly used method in metabolomics data analysis, is an extension of PLS-DA. Compared to the PLS-DA, this method can effectively reduce the complexity of the model and enhance the explanatory power of the model without reducing the predictive power, thus maximizing the differences between groups.

3 Analysis and identification of metabolites

3.1 Differential metabolite screening

Metabolites are screened to identify differential metabolites (biomarkers); the relevant conditions are as follows: p-value ≤0.05 + VIP (variable importance for the projection) ≥1.

3.2 Identification of metabolites

Metabolite identification was first confirmed on the basis of precise molecular weights (molecular weight error <30 ppm), followed by confirmation of annotation against the Metlin (http://metlin.scripps.edu) and MoNA (https://mona.fiehnlab.ucdavis.edu//) databases based on MS/MS fragmentation patterns to identify the final metabolites.

3.3 Network analysis

Metscape [41], a Cytoscape plug-in (v.3.9.0) [42], was used for the metabolic network analysis and data visualization.

3.4 Kyoto encyclopedia of genes and genomes (KEGG) analysis

MetPA is part of metaboanalyst (www.metaboanalyst.ca) and is based on the KEGG metabolic pathway. The MetPA database identifies possible bioturbated metabolic pathways through metabolic pathway enrichment and topology analysis, and thus analyzes the metabolic pathways of metabolites. The MetPA database allows the analysis of metabolic pathways associated with two sets of differential metabolites, using a hypergeometric test as the data analysis algorithm, and relative-betweeness centrality for pathway topology.

4 Results

4.1 Subject characteristics

Ten healthy pregnant women of the same trimester and 10 pregnant women with severe PE were included in this study. There were no significant differences between the two groups when subject characteristics such as age (31.6 ± 5.77) or week of gestation (32.1 ± 1.72 W) were taken into account. However, there were significant differences in BP, gestational week at termination, neonatal Apgar score, and neonatal weight. Table 1 shows the clinical features of the 10 severe PE cases.

Table 1

Severe PE clinical features

NO Age (years) Gestational weeks Systolic pressure (mmHg) Edema 24 h urine protein (g/L) Albumin Ultrasound of pleural and peritoneal effusion Time to terminate pregnancy Neonatal Apgar Scores Neonatal weight (g) Echocard-iography
1 25 33 + 3 116–151 Normal 11.120 22.6 g/L Normal 34 weeks 8–10 1,600 Normal
2 32 29 + 5 109–161 (+) 0.603 Normal Normal 30 + 6 9–10 1,270 Normal
3* 31 31 + 5 100–139 (+) 0.378 25.2 g/L Normal 35 + 1 9–10 2,160 and 2,420 (twins) Normal
4 29 32 + 3 120–160 Normal 1.229 Normal Normal Leave hospital Leave hospital Leave hospital Normal
5 33 31 + 2 150–175 Normal Leave hospital Leave hospital Normal Leave hospital Leave hospital Leave hospital Normal
6 45 32 + 6 107–166 Normal 5.292 24 g/L Normal 32 + 6 7–10 1,250 Normal
7 31 29 130–170 Normal 6.134 Normal Normal 29 + 2 0 1,050 ＜5 mL
8 24 32 + 6 122–161 (++++) 4.772 27.2 g/L Pleural effusion 33 + 2 9–10 1,640 Normal
9 34 33 + 3 110–147 (++) 0.454 25.1 g/L Pleural effusion 33 + 3 8–10 1,720 and 1,790 (twins) Normal
10 34 34 + 3 118–175 (+++) 1.742 26.5 g/L Pleural effusion 34 + 6 8–10 1,920 and 1,890 (twins) Normal

Note: NO 7, The patient requested induction of labor, rivanol amniotic injection at 29 + 2 weeks, neonatal death. No 3 had a diastolic BP of 110 mmHg. *Had a diastolic BP of 110 mmHg.

4.2 Multivariate analysis

In this study, serum samples from the 10 healthy pregnant women and 10 severe PE patients were analyzed with a metabolomics approach, using GC-MS followed by the multivariate data analysis by PCA, PLS-DA, and OPLS-DA (Table 2). Initial unsupervised PCA (Figure 1a) and supervised PCA (Figure 1b and c) showed a clear separation of metabolites between the normal and severe EOPE groups.

Table 2

Multivariate data analysis result

PE vs Normal Pre R2X (cum) R2Y (cum) Q2 (cum)
PCA 4 0.511
PLS-DA 3 0.432 0.998 0.962
OPLS-DA 1 + 1 + 0 0.356 0.973 0.895
Figure 1 (a) PCA, (b) PLS-DA, and (c) OPLS-DA of the severe EOPE and normal groups.
Figure 1

(a) PCA, (b) PLS-DA, and (c) OPLS-DA of the severe EOPE and normal groups.

4.3 Identified metabolites

In the GC-MS analysis, 3138 differential metabolites were screened and 124 metabolites (Figure 2) were eventually identified. Of the 124 identified differential metabolites, 45 downregulated products and 79 upregulated products were included. The classification of the identified differential metabolites are summarized in Figure 3; these were mainly located on metabolism of amino acids, carbohydrates, cofactors and vitamins, lipids, nucleotides, and peptides.

Figure 2 Heatmap with all the significant metabolites.
Figure 2

Heatmap with all the significant metabolites.

Figure 3 Classification of identified differential metabolites. The differential metabolites located on metabolism of amino acids, carbohydrates, cofactors and vitamins, lipids, nucleotides, and peptides.
Figure 3

Classification of identified differential metabolites. The differential metabolites located on metabolism of amino acids, carbohydrates, cofactors and vitamins, lipids, nucleotides, and peptides.

4.4 Network analysis

By building an association-based network, we found that 2-hydroxybutyric acid was important within this metabolic network (Figure 4). Furthermore, the results of the differential metabolite analysis showed that 2-hydroxybutyric acid differed significantly between preeclamptic and normal pregnant women (VIP = 1.670, p-value <0.05, false discovery rate (FDR) = 0.006, log FC = 1.016). Meanwhile, the results of analysis of variance for l-threonine and 5,6-dihydro-5-fluorouracil were VIP = 1.695, p-value <0.05, FDR = 0.022, log FC = 1.036; and VIP = 1.654, p-value <0.05, FDR = 0.006, log FC = 1.020, respectively. In the ROC plot (Figure 5), there was an area under the curve (AUC) of 0.99, indicating a high level of accuracy (high accuracy = AUC >0.9).

Figure 4 Network analysis of identified differential metabolites. 2-Hydroxybutyric acid was identified as the key metabolite in a network analysis based on VIP and FDR values.
Figure 4

Network analysis of identified differential metabolites. 2-Hydroxybutyric acid was identified as the key metabolite in a network analysis based on VIP and FDR values.

Figure 5 ROC plot of 2-hydroxybutyric acid and the concentrations of 2-hydroxybutyric acid in PE group and normal group. (a) There was an AUC of 0.99. (b) The concentrations of 2-hydroxybutyric acid is 47559849.097 ± 14839964.376 in the PE group and 23509331.240 ± 4802006.390 in the normal group.
Figure 5

ROC plot of 2-hydroxybutyric acid and the concentrations of 2-hydroxybutyric acid in PE group and normal group. (a) There was an AUC of 0.99. (b) The concentrations of 2-hydroxybutyric acid is 47559849.097 ± 14839964.376 in the PE group and 23509331.240 ± 4802006.390 in the normal group.

4.5 KEGG analysis

The final results of the differential metabolite KEGG pathway enrichment analysis are shown in Figure 6. Significantly enriched pathways included central carbon metabolism in cancer, protein digestion and absorption, aminoacyl-transfer RNA (tRNA) biosynthesis, mineral absorption, alanine, aspartate and glutamate metabolism, and prostate cancer (FDR <0.05).

Figure 6 KEGG pathway analysis of identified differential metabolites. Significantly enriched pathways include central carbon metabolism in cancer (37 differential metabolites); protein digestion and absorption (47 differential metabolites); aminoacyl-tRNA biosynthesis (52 differential metabolites); mineral absorption (29 differential metabolites); alanine, aspartate, and glutamate metabolism (28 differential metabolites); prostate cancer (11 differential metabolites) (FDR <0.05). The size of the circle represents the number of differential metabolites that are enriched in this pathway. −log(p): Negative values for the natural logarithm of the p-value. Impact: Metabolic pathway impact values.
Figure 6

KEGG pathway analysis of identified differential metabolites. Significantly enriched pathways include central carbon metabolism in cancer (37 differential metabolites); protein digestion and absorption (47 differential metabolites); aminoacyl-tRNA biosynthesis (52 differential metabolites); mineral absorption (29 differential metabolites); alanine, aspartate, and glutamate metabolism (28 differential metabolites); prostate cancer (11 differential metabolites) (FDR <0.05). The size of the circle represents the number of differential metabolites that are enriched in this pathway. −log(p): Negative values for the natural logarithm of the p-value. Impact: Metabolic pathway impact values.

5 Discussion

In this study, we analyzed the metabolites in the sera of 10 severe PE cases and 10 healthy pregnant women. A total of 3,138 differential metabolites were screened, resulting in the identification of 124 differential metabolites. After analysis of 124 differential metabolites, 2-hydroxybutyric acid was found to be the more critical differential metabolite; its presence clearly distinguished between severe PE and healthy pregnant women. The analysis of the KEGG pathway revealed that the metabolites were mainly enriched in the following metabolic pathways: central carbon metabolism in cancer; protein digestion and absorption; aminoacyl-tRNA biosynthesis; mineral absorption; alanine, aspartate, and glutamate metabolism; prostate cancer.

2-Hydroxybutyric acid is predominantly produced during the metabolism of l-threonine or the synthesis of glutathione and may be elevated by oxidative stress or the detoxification of exogenous substances in the liver [43]. It has been previously demonstrated that 2-hydroxybutyric acid, as a component of a metabolite-only model, can predict the EOPE [44]. Our study also found that this metabolite played an important role in severe PE; however, we used a different approach.

Two of the KEGG enrichment analyses differential metabolites that we studied were associated with cancer. The other four KEGG pathways were all found to be associated with severe PE. The differential mRNAs between the preeclamptic and normal groups were also found to be enriched in the protein digestion and absorption pathways [45]. Thus, previous studies have found that l-arginine supplementation can be used to treat individuals with PE [46]. Harville et al. demonstrated that aminoacyl-tRNA biosynthesis is associated with hypertensive disorders of pregnancy [47]. Furthermore, the relationship between minerals and PE has been relatively well studied. Serum selenium levels have been shown to be associated with PE in several studies [48–51]; however, amniotic fluid selenium status has been shown to be uncorrelated with PE or preterm delivery [52,53]. On the other hand, Enebe et al. found that low levels of antioxidant trace elements, for example, selenium, copper, and magnesium, can promote the incidence of PE [54], and other studies have found that mineral and vitamin supplementation can reduce the incidence [55,56]. These findings illustrate the impact of mineral absorption pathways on PE. To our knowledge, there is no literature addressing the relationship between the alanine, aspartate, and glutamate metabolism pathway and PE. However, a proportion of patients with PE do have abnormal liver function.

In summary, our analysis revealed that 2-hydroxybutyric acid might be a key metabolite for distinguishing severe PE from normal controls and is potentially a marker for the early diagnosis of severe PE, thus providing a basis for early detection and intervention.

Acknowledgments

We thank Dr Huan Chen for his guidance in writing the manuscript.

  1. Funding information: This manuscript was supported by the Hunan Provincial Natural Science Foundation (Grant No. 2019JJ60089).

  2. Author contributions: Fang Wang and Kaili Xie contributed to the conception of the study; Mingming Qi and Xihua Ma performed the experiment; Lili Xu and Furong Liang contributed significantly to analysis and manuscript preparation; Huimin Lai and Chan Zhang performed the data analyses and wrote the manuscript; Fanhua Zeng and Qing Wen helped perform the analyses and contributed to constructive discussions.

  3. Conflict of interest: Authors state no conflict of interest.

  4. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

[1] Deer E, Jones J, Cornelius DC, Comley K, Herrock O, Campbell N, et al. Progesterone induced blocking factor reduces hypertension and placental mitochondrial dysfunction in response to sFlt-1 during pregnancy. Cells. 2021 Oct 20;10(11):2817. 10.3390/cells10112817. PMID: 34831040. PMCID: PMC8616090.Search in Google Scholar PubMed PubMed Central

[2] Tianthong W, Phupong V. Serum hypoxia-inducible factor-1α and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia. Sci Rep. 2021 Mar 23;11(1):6674. 10.1038/s41598-021-86073-w. PMID: 33758274. PMCID: PMC7988168.Search in Google Scholar PubMed PubMed Central

[3] Daniels K, Lewin S, Practice Policy Group. Translating research into maternal health care policy: a qualitative case study of the use of evidence in policies for the treatment of eclampsia and pre-eclampsia in South Africa. Health Res Policy Syst. 2008 Dec 17;6:12. 10.1186/1478-4505-6-12. PMID: 19091083. PMCID: PMC2645395.Search in Google Scholar PubMed PubMed Central

[4] Birhanu MY, Temesgen H, Demeke G, Assemie MA, Alamneh AA, Desta M, et al. Incidence and predictors of pre-eclampsia among pregnant women attending antenatal care at debre markos referral hospital, North West Ethiopia: Prospective cohort study. Int J Womens Health. 2020 Nov 10;12:1013–21. 10.2147/IJWH.S265643. PMID: 33204174. PMCID: PMC7667502.Search in Google Scholar PubMed PubMed Central

[5] Eddy AC, Bidwell GL 3rd, George EM. Pro-angiogenic therapeutics for preeclampsia. Biol Sex Differ. 2018 Aug 25;9(1):36. 10.1186/s13293-018-0195-5. PMID: 30144822. PMCID: PMC6109337.Search in Google Scholar PubMed PubMed Central

[6] Ge J, Wang J, Xue D, Zhu Z, Chen Z, Li X, et al. Why does a high-fat diet induce preeclampsia-like symptoms in pregnant rats. Neural Regen Res. 2013 Jul 15;8(20):1872–80. 10.3969/j.issn.1673-5374.2013.20.006. PMID: 25206496. PMCID: PMC4145971.Search in Google Scholar PubMed PubMed Central

[7] Savage AR, Hoho L. Knowledge of pre-eclampsia in women living in Makole Ward, Dodoma, Tanzania. Afr Health Sci. 2016 Jun;16(2):412–9. 10.4314/ahs.v16i2.9. PMID: 27605956. PMCID: PMC4994549.Search in Google Scholar PubMed PubMed Central

[8] Tang Y, Ye W, Liu X, Lv Y, Yao C, Wei J. VEGF and sFLT-1 in serum of PIH patients and effects on the foetus. Exp Ther Med. 2019 Mar;17(3):2123–8. 10.3892/etm.2019.7184. Epub 2019 Jan 18 PMID: 30867699. PMCID: PMC6396009.Search in Google Scholar PubMed PubMed Central

[9] Tarca AL, Romero R, Benshalom-Tirosh N, Than NG, Gudicha DW, Done B, et al. The prediction of early preeclampsia: Results from a longitudinal proteomics study. PLoS One. 2019 Jun 4;14(6):e0217273. 10.1371/journal.pone.0217273. PMID: 31163045. PMCID: PMC6548389.Search in Google Scholar PubMed PubMed Central

[10] Blair JD, Yuen RK, Lim BK, McFadden DE, von Dadelszen P, Robinson WP. Widespread DNA hypomethylation at gene enhancer regions in placentas associated with early-onset pre-eclampsia. Mol Hum Reprod. 2013 Oct;19(10):697–708. 10.1093/molehr/gat044. Epub 2013 Jun 13 PMID: 23770704. PMCID: PMC3779005.Search in Google Scholar PubMed PubMed Central

[11] Wen YH, Yang HI, Chou HC, Chen CY, Hsieh WS, Tsou KI, et al. Taiwan Premature Infant Developmental Collaborative Study Group. Association of maternal preeclampsia with neonatal respiratory distress syndrome in very-low-birth-weight infants. Sci Rep. 2019 Sep 13;9(1):13212. 10.1038/s41598-019-49561-8. PMID: 31519996. PMCID: PMC6744454.Search in Google Scholar PubMed PubMed Central

[12] Park HJ, Kim SH, Jung YW, Shim SS, Kim JY, Cho YK, et al. Screening models using multiple markers for early detection of late-onset preeclampsia in low-risk pregnancy. BMC Pregnancy Childbirth. 2014 Jan 20;14:35. 10.1186/1471-2393-14-35. PMID: 24444293. PMCID: PMC3944217.Search in Google Scholar PubMed PubMed Central

[13] Morikawa M, Kato-Hirayama E, Mayama M, Saito Y, Nakagawa K, Umazume T, et al. Glycemic control and fetal growth of women with diabetes mellitus and subsequent hypertensive disorders of pregnancy. PLoS One. 2020 Mar 16;15(3):e0230488. 10.1371/journal.pone.0230488. PMID: 32176740. PMCID: PMC7075561.Search in Google Scholar PubMed PubMed Central

[14] Vizitiu R, Krauss-Grignard M, Garcia V, Valentin L, Samain E, Diemunsch P. Urapidyl for hypertension control in severe pre-eclampsia: a comparative study with nicardipine. Crit Care. 2010;14(Suppl 1):P136. 10.1186/cc8368. Epub 2010 Mar 1 PMCID: PMC2934274.Search in Google Scholar

[15] Mrema D, Lie RT, Østbye T, Mahande MJ, Daltveit AK. The association between pre pregnancy body mass index and risk of preeclampsia: a registry based study from Tanzania. BMC Pregnancy Childbirth. 2018 Feb 21;18(1):56. 10.1186/s12884-018-1687-3. PMID: 29466949. PMCID: PMC5822591.Search in Google Scholar PubMed PubMed Central

[16] Grum T, Seifu A, Abay M, Angesom T, Tsegay L. Determinants of pre-eclampsia/Eclampsia among women attending delivery Services in Selected Public Hospitals of Addis Ababa, Ethiopia: a case control study. BMC Pregnancy Childbirth. 2017 Sep 15;17(1):307. 10.1186/s12884-017-1507-1. PMID: 28915802. PMCID: PMC5603094.Search in Google Scholar PubMed PubMed Central

[17] Roland CS, Hu J, Ren CE, Chen H, Li J, Varvoutis MS, et al. Morphological changes of placental syncytium and their implications for the pathogenesis of preeclampsia. Cell Mol Life Sci. 2016 Jan;73(2):365–76. 10.1007/s00018-015-2069-x. Epub 2015 Oct 26 PMID: 26496726. PMCID: PMC4846582.Search in Google Scholar PubMed PubMed Central

[18] Wang J, Zhou L, Lei H, Hao F, Liu X, Wang Y, et al. Simultaneous quantification of amino metabolites in multiple metabolic pathways using ultra-high performance liquid chromatography with tandem-mass spectrometry. Sci Rep. 2017 May 3;7(1):1423. 10.1038/s41598-017-01435-7. PMID: 28469184. PMCID: PMC5431165.Search in Google Scholar PubMed PubMed Central

[19] Cruickshank-Quinn C, Zheng LK, Quinn K, Bowler R, Reisdorph R, Reisdorph N. Impact of blood collection tubes and sample handling time on serum and plasma metabolome and lipidome. Metabolites. 2018 Dec 4;8(4):88. 10.3390/metabo8040088. PMID: 30518126. PMCID: PMC6316012.Search in Google Scholar PubMed PubMed Central

[20] Liang Y, Ke X, Xiao Z, Zhang Y, Chen Y, Li Y, et al. Untargeted metabolomic profiling using UHPLC-QTOF/MS reveals metabolic alterations associated with autism. Biomed Res Int. 2020 Sep 11;2020:6105608. 10.1155/2020/6105608. PMID: 32964039. PMCID: PMC7502129.Search in Google Scholar PubMed PubMed Central

[21] Liu M, Tang L, Liu X, Fang J, Zhan H, Wu H, et al. An evidence-based review of related metabolites and metabolic network research on cerebral ischemia. Oxid Med Cell Longev. 2016;2016:9162074. 10.1155/2016/9162074. Epub 2016 May 5 PMID: 27274780. PMCID: PMC4871976.Search in Google Scholar PubMed PubMed Central

[22] Utermann C, Parrot D, Breusing C, Stuckas H, Staufenberger T, Blümel M, et al. Combined genotyping, microbial diversity and metabolite profiling studies on farmed Mytilus spp. from Kiel Fjord. Sci Rep. 2018 May 22;8(1):7983. 10.1038/s41598-018-26177-y. PMID: 29789708. PMCID: PMC5964093.Search in Google Scholar PubMed PubMed Central

[23] Wang D, Shi C, Ge ZH, Wei YX, Liu TT, Wang Y, et al. Study of the mechanism of action of Guanxin Shutong capsules in the treatment of coronary heart disease based on metabolomics. Front Pharmacol. 2021 Mar 25;12:650438. 10.3389/fphar.2021.650438. PMID: 33867992. PMCID: PMC8048374.Search in Google Scholar PubMed PubMed Central

[24] Lin R, Piao M, Song Y, Liu C. Quercetin suppresses AOM/DSS-induced colon carcinogenesis through its anti-inflammation effects in mice. J Immunol Res. 2020 May 21;2020:9242601. 10.1155/2020/9242601. PMID: 32537472. PMCID: PMC7260625.Search in Google Scholar PubMed PubMed Central

[25] Liu Y, Guo JZ, Liu Y, Wang K, Ding W, Wang H, et al. Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun. 2018 Oct 24;9(1):4429. 10.1038/s41467-018-06841-7. PMID: 30356100. PMCID: PMC6200739.Search in Google Scholar PubMed PubMed Central

[26] Kim HK, Son SY, Oh JS, Song YN, Byun JM, Koh Y, et al. Metabolic profiling during acute myeloid leukemia progression using paired clinical bone marrow serum samples. Metabolites. 2021 Aug 31;11(9):586. 10.3390/metabo11090586. PMID: 34564403. PMCID: PMC8471543.Search in Google Scholar PubMed PubMed Central

[27] Bruzzone C, Bizkarguenaga M, Gil-Redondo R, Diercks T, Arana E, García de Vicuña A, et al. SARS-CoV-2 infection dysregulates the metabolomic and lipidomic profiles of serum. iScience. 2020 Oct 23;23(10):101645. 10.1016/j.isci.2020.101645. Epub 2020 Oct 5. PMID: 33043283. PMCID: PMC7534591.Search in Google Scholar PubMed PubMed Central

[28] Liu Z, Li J, Li M, Chen S, Gao R, Zeng G, et al. Elevated α-hydroxybutyrate dehydrogenase as an independent prognostic factor for mortality in hospitalized patients with COVID-19. ESC Heart Fail. 2021 Feb;8(1):644–51. 10.1002/ehf2.13151. Epub 2020 Dec 17. PMID: 33336560. PMCID: PMC7835619.Search in Google Scholar PubMed PubMed Central

[29] Zhou B, Lou B, Liu J, She J. Serum metabolite profiles as potential biochemical markers in young adults with community-acquired pneumonia cured by moxifloxacin therapy. Sci Rep. 2020 Mar 10;10(1):4436. 10.1038/s41598-020-61290-x. PMID: 32157124. PMCID: PMC7064523.Search in Google Scholar PubMed PubMed Central

[30] Li X, Xu Z, Lu X, Yang X, Yin P, Kong H, et al. Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus. Anal Chim Acta. 2009 Feb 9;633(2):257–62. 10.1016/j.aca.2008.11.058. Epub 2008 Dec 3. PMID: 19166731.Search in Google Scholar PubMed

[31] Sousa AP, Cunha DM, Franco C, Teixeira C, Gojon F, Baylina P, et al. Which role plays 2-hydroxybutyric acid on insulin resistance? Metabolites. 2021 Dec 3;11(12):835. 10.3390/metabo11120835. PMID: 34940595. PMCID: PMC8703345.Search in Google Scholar PubMed PubMed Central

[32] Cobb J, Eckhart A, Motsinger-Reif A, Carr B, Groop L, Ferrannini E. α-Hydroxybutyric acid is a selective metabolite biomarker of impaired glucose tolerance. Diabetes Care. 2016 Jun;39(6):988–95. 10.2337/dc15-2752. Epub 2016 Apr 5. PMID: 27208342.Search in Google Scholar PubMed

[33] Tricò D, Prinsen H, Giannini C, de Graaf R, Juchem C, Li F, et al. Elevated α-hydroxybutyrate and branched-chain amino acid levels predict deterioration of glycemic control in adolescents. J Clin Endocrinol Metab. 2017 Jul 1;102(7):2473–81. 10.1210/jc.2017-00475. PMID: 28482070. PMCID: PMC5505187.Search in Google Scholar PubMed PubMed Central

[34] Chou J, Liu R, Yu J, Liu X, Zhao X, Li Y, et al. Fasting serum α-hydroxybutyrate and pyroglutamic acid as important metabolites for detecting isolated post-challenge diabetes based on organic acid profiles. J Chromatogr B Anal Technol Biomed Life Sci. 2018 Nov 15;1100–1101:6–16. 10.1016/j.jchromb.2018.09.004. Epub 2018 Sep 4. PMID: 30267980.Search in Google Scholar PubMed

[35] Yoshida N, Kitahama S, Yamashita T, Hirono Y, Tabata T, Saito Y, et al. Metabolic alterations in plasma after laparoscopic sleeve gastrectomy. J Diabetes Investig. 2021 Jan;12(1):123–9. 10.1111/jdi.13328. Epub 2020 Jul 26. PMID: 32563200. PMCID: PMC7779268.Search in Google Scholar PubMed PubMed Central

[36] Nemutlu E, Orgul G, Recber T, Aydin E, Ozkan E, Turgal M, et al. Metabolic infrastructure of pregnant women with trisomy 21 fetuses; Metabolomic analysis. Z Geburtshilfe Neonatol. 2019 Oct;223(5):297–303. 10.1055/a-0877-7869. Epub 2019 May 27 PMID: 31132797.Search in Google Scholar PubMed

[37] Martin-Lorenzo M, Ramos-Barron A, Gutierrez-Garcia P, Martin-Blazquez A, Santiago-Hernandez A, Rodrigo Calabia E, et al. Urinary spermidine predicts and associates with in-hospital acute kidney injury after cardiac surgery. Antioxid (Basel). 2021 Jun 2;10(6):896. 10.3390/antiox10060896. PMID: 34199603. PMCID: PMC8229689.Search in Google Scholar PubMed PubMed Central

[38] Pu J, Liu Y, Zhang H, Tian L, Gui S, Yu Y, et al. An integrated meta-analysis of peripheral blood metabolites and biological functions in major depressive disorder. Mol Psychiatry. 2021 Aug;26(8):4265–76. 10.1038/s41380-020-0645-4. Epub 2020 Jan 20. PMID: 31959849. PMCID: PMC8550972.Search in Google Scholar PubMed PubMed Central

[39] Boulesteix AL, Strimmer K. Partial least squares: a versatile tool for the analysis of high-dimensional genomic data. Brief Bioinforma. 2007;8:32–44.10.1093/bib/bbl016Search in Google Scholar PubMed

[40] Thevenot EA, Roux A, Xu Y, Ezan E, Junot C. Analysis of the human adult urinary metabolome variations with age, body mass index and gender by implementing a comprehensive workflow for univariate and OPLS statistical analyses. J Proteome Res. 2015;14:3322–35.10.1021/acs.jproteome.5b00354Search in Google Scholar PubMed

[41] Karnovsky A, Weymouth T, Hull T, Tarcea VG, Scardoni G, Laudanna C, et al. Metscape 2 bioinformatics tool for the analysis and visualization of metabolomics and gene expression data. Bioinformatics. 2012 Feb 1;28(3):373–80. 10.1093/bioinformatics/btr661. Epub 2011 Nov 30 PMID: 22135418. PMCID: PMC3268237.Search in Google Scholar PubMed PubMed Central

[42] Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 2003 Nov;13(11):2498–504. 10.1101/gr.1239303. PMID: 14597658. PMCID: PMC403769.Search in Google Scholar PubMed PubMed Central

[43] Shi D, Yan R, Lv L, Jiang H, Lu Y, Sheng J, et al. The serum metabolome of COVID-19 patients is distinctive and predictive. Metabolism. 2021 May;118:154739. 10.1016/j.metabol.2021.154739. Epub 2021 Mar 2. PMID: 33662365. PMCID: PMC7920809.Search in Google Scholar PubMed PubMed Central

[44] Bahado-Singh RO, Syngelaki A, Akolekar R, Mandal R, Bjondahl TC, Han B, et al. Validation of metabolomic models for prediction of early-onset preeclampsia. Am J Obstet Gynecol. 2015 Oct;213(4):530.e1–530.e10. 10.1016/j.ajog.2015.06.044. Epub 2015 Jun 23 PMID: 26116099.Search in Google Scholar PubMed

[45] He J, Liu K, Hou X, Lu J. Identification and validation of key non-coding RNAs and mRNAs using co-expression network analysis in pre-eclampsia. Med (Baltim). 2021 Apr 9;100(14):e25294. 10.1097/MD.0000000000025294. PMID: 33832098. PMCID: PMC8036074.Search in Google Scholar PubMed PubMed Central

[46] Wu G, Meininger CJ, McNeal CJ, Bazer FW, Rhoads JM. Role of L-arginine in nitric oxide synthesis and health in humans. Adv Exp Med Biol. 2021;1332:167–87. 10.1007/978-3-030-74180-8_10. PMID: 34251644.Search in Google Scholar PubMed

[47] Harville EW, Li YY, Pan K, McRitchie S, Pathmasiri W, Sumner S. Untargeted analysis of first trimester serum to reveal biomarkers of pregnancy complications: a case-control discovery phase study. Sci Rep. 2021;11(1):3468. Published 2021 Feb 10. 10.1038/s41598-021-82804-1.Search in Google Scholar PubMed PubMed Central

[48] Ghaemi SZ, Forouhari S, Dabbaghmanesh MH, Sayadi M, Bakhshayeshkaram M, Vaziri F, et al. A prospective study of selenium concentration and risk of preeclampsia in pregnant Iranian women: a nested case-control study. Biol Trace Elem Res. 2013 May;152(2):174–9. 10.1007/s12011-013-9614-y. Epub 2013 Jan 26 PMID: 23354545.Search in Google Scholar PubMed

[49] Haque MM, Moghal MM, Sarwar MS, Anonna SN, Akter M, Karmakar P, et al. Low serum selenium concentration is associated with preeclampsia in pregnant women from Bangladesh. J Trace Elem Med Biol. 2016 Jan;33:21–5. 10.1016/j.jtemb.2015.08.002. Epub 2015 Aug 20 PMID: 26653739.Search in Google Scholar PubMed

[50] Maleki A, Fard MK, Zadeh DH, Mamegani MA, Abasaizadeh S, Mazloomzadeh S. The relationship between plasma level of Se and preeclampsia. Hypertens Pregnancy. 2011;30(2):180–7. 10.3109/10641950903322931. Epub 2010 Sep 6 PMID: 20818958.Search in Google Scholar PubMed

[51] Eze SC, Ododo NA, Ugwu EO, Enebe JT, Onyegbule OA, Eze IO, et al. Serum selenium levels of pre-eclamptic and normal pregnant women in Nigeria: A comparative study. PLoS One. 2020 Aug 27;15(8):e0238263. 10.1371/journal.pone.0238263. PMID: 32853288. PMCID: PMC7451566.Search in Google Scholar PubMed PubMed Central

[52] Roy AC, Ratnam SS, Karunanithy R. Amniotic fluid selenium status in pre-eclampsia. Gynecol Obstet Invest. 1989;28(3):161–2. 10.1159/000293557. PMID: 2807037.Search in Google Scholar PubMed

[53] Ogrizek Pelkič K, Sobočan M, Takač I. Low selenium levels in amniotic fluid correlate with small-for-gestational age newborns. Nutrients. 2020 Oct 5;12(10):3046. 10.3390/nu12103046. PMID: 33027985. PMCID: PMC7600462.Search in Google Scholar PubMed PubMed Central

[54] Enebe JT, Dim CC, Ugwu EO, Enebe NO, Meka IA, Obioha KC, et al. Serum antioxidant micronutrient levels in pre-eclamptic pregnant women in Enugu, south-East Nigeria: a comparative cross-sectional analytical study. BMC Pregnancy Childbirth. 2020 Jul 6;20(1):392. 10.1186/s12884-020-03081-w. PMID: 32631273. PMCID: PMC7339396.Search in Google Scholar PubMed PubMed Central

[55] Hovdenak N, Haram K. Influence of mineral and vitamin supplements on pregnancy outcome. Eur J Obstet Gynecol Reprod Biol. 2012 Oct;164(2):127–32. 10.1016/j.ejogrb.2012.06.020. Epub 2012 Jul 6 PMID: 22771225.Search in Google Scholar PubMed

[56] Omotayo MO, Dickin KL, O’Brien KO, Neufeld LM, De Regil LM, Stoltzfus RJ. Calcium supplementation to prevent preeclampsia: Translating guidelines into practice in low-income countries. Adv Nutr. 2016 Mar 15;7(2):275–8. 10.3945/an.115.010736. PMID: 26980810. PMCID: PMC4785477.Search in Google Scholar PubMed PubMed Central

Received: 2022-09-23
Revised: 2022-12-16
Accepted: 2023-01-14
Published Online: 2023-02-28

© 2023 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  217. Bamboo charcoal affects soil properties and bacterial community in tea plantations
  218. Optimization of biogas potential using kinetic models, response surface methodology, and instrumental evidence for biodegradation of tannery fleshings during anaerobic digestion
  219. Understory vegetation diversity patterns of Platycladus orientalis and Pinus elliottii communities in Central and Southern China
  220. Studies on macrofungi diversity and discovery of new species of Abortiporus from Baotianman World Biosphere Reserve
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  223. Guesstimate of thymoquinone diversity in Nigella sativa L. genotypes and elite varieties collected from Indian states using HPTLC technique
  224. Analysis of bacterial community structure of Fuzhuan tea with different processing techniques
  225. Untargeted metabolomics reveals sour jujube kernel benefiting the nutritional value and flavor of Morchella esculenta
  226. Mycobiota in Slovak wine grapes: A case study from the small Carpathians wine region
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  238. On correlation between canopy vegetation and growth indexes of maize varieties with different nitrogen efficiencies
  239. Exopolysaccharides from Pseudomonas tolaasii inhibit the growth of Pleurotus ostreatus mycelia
  240. A transcriptomic evaluation of the mechanism of programmed cell death of the replaceable bud in Chinese chestnut
  241. Melatonin enhances salt tolerance in sorghum by modulating photosynthetic performance, osmoregulation, antioxidant defense, and ion homeostasis
  242. Effects of plant density on alfalfa (Medicago sativa L.) seed yield in western Heilongjiang areas
  243. Identification of rice leaf diseases and deficiency disorders using a novel DeepBatch technique
  244. Artificial intelligence and internet of things oriented sustainable precision farming: Towards modern agriculture
  245. Animal Sciences
  246. Effect of ketogenic diet on exercise tolerance and transcriptome of gastrocnemius in mice
  247. Combined analysis of mRNA–miRNA from testis tissue in Tibetan sheep with different FecB genotypes
  248. Isolation, identification, and drug resistance of a partially isolated bacterium from the gill of Siniperca chuatsi
  249. Tracking behavioral changes of confined sows from the first mating to the third parity
  250. The sequencing of the key genes and end products in the TLR4 signaling pathway from the kidney of Rana dybowskii exposed to Aeromonas hydrophila
  251. Development of a new candidate vaccine against piglet diarrhea caused by Escherichia coli
  252. Plant Sciences
  253. Crown and diameter structure of pure Pinus massoniana Lamb. forest in Hunan province, China
  254. Genetic evaluation and germplasm identification analysis on ITS2, trnL-F, and psbA-trnH of alfalfa varieties germplasm resources
  255. Tissue culture and rapid propagation technology for Gentiana rhodantha
  256. Effects of cadmium on the synthesis of active ingredients in Salvia miltiorrhiza
  257. Cloning and expression analysis of VrNAC13 gene in mung bean
  258. Chlorate-induced molecular floral transition revealed by transcriptomes
  259. Effects of warming and drought on growth and development of soybean in Hailun region
  260. Effects of different light conditions on transient expression and biomass in Nicotiana benthamiana leaves
  261. Comparative analysis of the rhizosphere microbiome and medicinally active ingredients of Atractylodes lancea from different geographical origins
  262. Distinguish Dianthus species or varieties based on chloroplast genomes
  263. Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury
  264. Study on fresh processing key technology and quality influence of Cut Ophiopogonis Radix based on multi-index evaluation
  265. An advanced approach for fig leaf disease detection and classification: Leveraging image processing and enhanced support vector machine methodology
  266. Erratum
  267. Erratum to “Protein Z modulates the metastasis of lung adenocarcinoma cells”
  268. Erratum to “BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells”
  269. Retraction
  270. Retraction to “Protocatechuic acid attenuates cerebral aneurysm formation and progression by inhibiting TNF-alpha/Nrf-2/NF-kB-mediated inflammatory mechanisms in experimental rats”
https://doi.org/10.1515/biol-2022-0572
Keywords for this article
severe preeclampsia; liquid chromatograph mass spectrometer; metabolomic analysis; 2-hydroxybutyric acid
Creative Commons
BY 4.0

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  252. Plant Sciences
  253. Crown and diameter structure of pure Pinus massoniana Lamb. forest in Hunan province, China
  254. Genetic evaluation and germplasm identification analysis on ITS2, trnL-F, and psbA-trnH of alfalfa varieties germplasm resources
  255. Tissue culture and rapid propagation technology for Gentiana rhodantha
  256. Effects of cadmium on the synthesis of active ingredients in Salvia miltiorrhiza
  257. Cloning and expression analysis of VrNAC13 gene in mung bean
  258. Chlorate-induced molecular floral transition revealed by transcriptomes
  259. Effects of warming and drought on growth and development of soybean in Hailun region
  260. Effects of different light conditions on transient expression and biomass in Nicotiana benthamiana leaves
  261. Comparative analysis of the rhizosphere microbiome and medicinally active ingredients of Atractylodes lancea from different geographical origins
  262. Distinguish Dianthus species or varieties based on chloroplast genomes
  263. Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury
  264. Study on fresh processing key technology and quality influence of Cut Ophiopogonis Radix based on multi-index evaluation
  265. An advanced approach for fig leaf disease detection and classification: Leveraging image processing and enhanced support vector machine methodology
  266. Erratum
  267. Erratum to “Protein Z modulates the metastasis of lung adenocarcinoma cells”
  268. Erratum to “BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells”
  269. Retraction
  270. Retraction to “Protocatechuic acid attenuates cerebral aneurysm formation and progression by inhibiting TNF-alpha/Nrf-2/NF-kB-mediated inflammatory mechanisms in experimental rats”
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