Startseite circ_0004904 regulates the trophoblast cell in preeclampsia via miR-19b-3p/ARRDC3 axis
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circ_0004904 regulates the trophoblast cell in preeclampsia via miR-19b-3p/ARRDC3 axis

  • Chenyuan Cao , Jie Cui und Guiling Liu EMAIL logo
Veröffentlicht/Copyright: 16. Mai 2023
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Open Medicine
Aus der Zeitschrift Open Medicine Band 18 Heft 1

Abstract

Circular RNAs have been demonstrated to act as vital participants in various diseases, including preeclampsia (PE). This study aimed to research the effects of circ_0004904 on PE. The contents of circ_0004904, microRNA-19b-3p (miR-19b-3p) and arrestin domain containing 3 (ARRDC3) were quantified by quantitative real-time PCR and western blot. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 5-ethynyl-2′-deoxyuridine assays were enforced to assess cell proliferation. The transwell assay and flow cytometry were applied to detect the cell migration, invasion, and apoptosis. The liaison between miR-19b-3p and circ_0004904 or ARRDC3 was demonstrated by dual-luciferase reporter assay. Thereafter, circ_0004904 and ARRDC3 were augmented, and miR-19b-3p was restrained in PE. Circ_0004904 silencing contributed to cell proliferation, migration, and invasion, but restrained cell apoptosis in trophoblast cells. Further, miR-19b-3p was a target of circ_0004904, and miR-19b-3p could target ARRDC3. Additionally, circ_0004904 accelerated PE evolution via changing ARRDC3 level by binding to miR-19b-3p. In all, circ_0004904 encouraged PE progress via miR-19b-3p/ARRDC3 axis.

Keywords: preeclampsia; circ_0004904; miR-19b-3p; ARRDC3

1 Introduction

Preeclampsia (PE) is a clinical condition characterized by hypertension, proteinuria, and edema, often occurring in the last trimester of pregnancy [1,2]. Its incidence is usually 3–7% of all pregnant women [3]. PE can be caused by many factors, such as high blood pressure, proteinuria, obesity, multiple pregnancies, and age at the time of conception [4]. If left untreated, PE can lead to maternal and newborn deaths [5]. At present, there are many doubts about the pathogenesis and regulatory mechanism of PE, and further research is needed.

Circular RNAs (circRNAs) are one of the endogenous RNAs with stable closed-loop structure, which have attracted extensive attention in disease regulation [6,7]. More importantly, some studies have found the abnormal expression of some circRNAs in the placenta of PE patients, which may be closely related to the pathogenesis of PE [8]. For instance, hsa_circ_0004904 and hsa_circ_0001855 were conspicuously intensified in PE, which might be the markers for PE forecasting [9]. However, the regulation mechanism of circ_0004904 in PE is still unclear, which will be the focus of this research. Moreover, hsa_circ_0036877 acted as a possible plasma biomarker for PE [10]. Besides, circHIPK3 participated in cell migration and tube formation in PE [11]. In addition, circ_0001438 took part in cell migration and apoptosis in PE [12]. In this work, we mainly studied the role of circ_0004904 in PE and its regulatory mechanism.

MicroRNAs (miRNAs) are diminutive noncoding RNAs and are concerned with numerous cell biological functions [13]. For example, miR-203a-3p and miR-548c-5p were essential participants in inflammatory response of PE [14,15]. Besides, miR-125a-5p repressed cell migration and proliferation in PE [16]. In addition, Sandrim et al. proved that microRNA-19b-3p (miR-19b-3p) expression was downregulated in PE [17]. But, the meaning and mechanism of miR-19b-3p in PE is not completely addressed.

Arrestin domain containing 3 (ARRDC3) is a member of the α-arrestin family [18]. Shen et al. reported that ARRDC3 contributed to YAP degradation, then accelerated colorectal cancer development [19]. Besides, ARRDC3 took part in the regulation of breast cancer [20]. Furthermore, ARRDC3 level was elevated and upregulated ARRDC3 lessened tube formation in PE [21]. Whereas, the regulation mechanism of ARRDC3 in PE is still unclear.

Here our results displayed that circ_0004904 was amplified in PE tissues and cells, and circ_0004904 silencing contributed to cell proliferation, migration, and invasion, but restrained cell apoptosis in trophoblast cells. Hence, we designed to recognize whether circ_0004904 could adjust PE growth via miR-19b-3p/ARRDC3 axis.

2 Materials and methods

2.1 Samples, cell culture, and transfection

The research was supervised by the Affiliated Hospital of Hebei University. 41 pairs PE placental tissues and normal placental tissues were gathered from The Affiliated Hospital of Hebei University. All volunteers signed the informed consent, and the present study was authorized by the Ethics Committee of The Affiliated Hospital of Hebei University.

Human trophoblast cell line (HTR-8/SVneo) was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The detailed process of cell culture was as the process reported by Gai et al. [22].

The si-circ_0004904, si-NC, miR-19b-3p mimic, miR-497-5p inhibitor, and controls, the pcDNA3.0-ARRDC3 and pcDNA3.0-NC, were from Sangon (Shanghai, China). The transfection was enforced by Lipofectamine 3000 (Solarbio, Beijing, China).

2.2 Quantitative real-time PCR (qRT-PCR) and RNA degradation assay

The TRIzol (Takara, Dalian, China) was utilized for RNA extraction. Then, the RNA was used for reverse transcription. After that, the qRT-PCR was enforced in line with the previous report [22]. GAPDH was utilized to standardize circ_0004904 and ARRDC3 levels, and U6 was employed to normalize miR-19b-3p content. All statistics were figured by 2−ΔΔCt method. RNase R (Solarbio) and Act D were applied to manifest the circular form of circ_0004904. The primers are listed in Table 1.

Table 1

Primers for qRT-PCR

Name Primers for qRT-PCR (5′−3′)
circ_0004904 Forward GAGGACCCACAGGCATGAAT
Reverse AGCGTGGAATATCAAATGCTCC
ARRDC3 Forward GGCACAAAAAGGGAGCGAAG
Reverse TGCAAATTCCCCTGAACGGA
miR-19b-3p Forward GCCGAGTGTGCAAATCCATGCT
Reverse CTCAACTGGTGTCGTGGA
GAPDH Forward TCCCATCACCATCTTCCAGG
Reverse GATGACCCTTTTGGCTCCC
U6 Forward CTCGCTTCGGCAGCACATATACT
Reverse ACGCTTCACGAATTTGCGTGTC
18S rRNA Forward CAGCCACCCGAGATTGAGCA
Reverse TAGTAGCGACGGGCGGTGTG
POLE2 Forward TGCCTCTGCATAAACCCTGG
Reverse TCTCAAAAGCCTTGAAGTTTGCT

2.3 Western blot

The technique of western blot was conducted as reported in a previous study [23]. The antibodies used for western blot were as follows: anti-ARRDC3 (ab64817; 1:500; Abcam, Cambridge, MA, USA), anti-cyclin E (ab33911; 1:1,000; Abcam), anti-vimentin (ab92547; 1:1,000; Abcam), anti-MMP2 (ab92536; 1:1,000; Abcam), anti-Bcl-2 (ab32124; 1:500; Abcam), anti-Bax (ab32503; 1:1,000; Abcam), and anti-GAPDH (ab8245; 1:5,000; Abcam).

2.4 Cell proliferation assay

HTR-8/SVneo cells (1 × 105/well) were loaded on 96-well plates. The MTT (Sigma) solution was employed as reported previously by Chen et al. [24]. Finally, the absorbance at 490 nm was observed with a microplate reader. Cell proliferation was also measured by Cell-Light™ Edu Kit (RiboBio, China) as stated in the directions.

2.5 Transwell assay

After transfection, the HTR-8/SVneo cells were supplemented into the above chamber of the transwell (Corning Inc., Corning, NY, USA) pre-coated with or without Matrigel (Corning) to assess the ability of cell invasion and migration, respectively. Then, the 200 µL of complete medium was added into the lower chamber. After 48 h, the moved cells were stained and calculated with a microscope.

2.6 Flow cytometry assay

HTR-8/SVneo cells were loaded on 6-well plates. The apoptotic cells were quantified via Annexin V-FITC Apoptosis Detection Kit (Sigma) and investigated using a flow cytometer.

2.7 ELISA assay

The activity of Caspase-3 was measured through human active Caspase-3 ELISA kit (ab181418; Abcam) in accordance with the specification.

2.8 Dual-luciferase reporter assay

The test was carried out based on the procedure reported by Gai et al. [22]. The connection between miR-19b-3p and circ_0004904 or ARRDC3 was predicted by starBase (http://starbase.sysu.edu.cn). Then, the circ_0004904 and ARRDC3 wild and mutant vectors were procured from Sangon (circ_0004904 WT, ARRDC3 3′UTR WT or circ_0004904 MUT, ARRDC3 3′UTR MUT).

2.9 RNA pull down assay

Bio-miR-19b-3p and Bio-miR-NC were from Sangon. HTR-8/SVneo cells were sonicated. The circ_0004904 probe was applied for cultivation with beads (Sigma) to produce probe-coated beads. Next cell lysates were incubated with circ_0004904 probe or oligo probe for 12 h. Then, the RNA stained with the beads was extracted for qRT-PCR.

2.10 Statistical assay

The tests were carried out in triplicate and the results were analyzed using SPSS 23.0 software (SPSS, USA). Student’s t-test and ANOVA were enforced to examine the differences. P < 0.05 was considered to be statistically significant.

  1. Ethics approval: The present study was approved by the ethical review committee of The Affiliated Hospital of Hebei University. Written informed consent was obtained from all enrolled patients.

  2. Consent for publication: Patients agree to participate in this work.

3 Results

3.1 Circ_0004904 was intensified in PE

Primarily, the outcomes displayed that in contrast to the normal tissues, circ_0004904 was intensified in PE placental tissues (Figure 1a). Circ_0004904 originates from the POLE2 gene and is located at chr14. Besides, circ_0004904 was formed by the exon 4–6 patchwork (Figure 1b). Moreover, the main distribution site of circ_0004904 was the cytoplasm (Figure 1c). Meanwhile, circ_0004904 content was not altered after RNase R or Act D treatment, while the POLE2 mRNA or GAPDH level was dropped (Figure 1d and e). In addition, circ_0004904 level was lower when Oligo (dT)18 primers were used compare with random primers, while the POLE2 mRNA was almost unchanged (Figure 1f). On the whole, these data demonstrated that circ_0004904 with a great steadiness structure was intensified in PE.

Figure 1 The relative level of circ_0004904 was enhanced in PE. (a) Relative levels of circ_0004904 in PE tissues and normal tissues were detected. (b) Location of circ_0004904 in genes and chromosomes. (c) Distribution of circ_0004904 in nucleus and cytoplasm. (d–f) Relative levels of circ_0004904 and POLE2 mRNA in HTR-8/SVneo cells. ***, P < 0.001.
Figure 1

The relative level of circ_0004904 was enhanced in PE. (a) Relative levels of circ_0004904 in PE tissues and normal tissues were detected. (b) Location of circ_0004904 in genes and chromosomes. (c) Distribution of circ_0004904 in nucleus and cytoplasm. (d–f) Relative levels of circ_0004904 and POLE2 mRNA in HTR-8/SVneo cells. ***, P < 0.001.

3.2 Circ_0004904 controlled trophoblast cell functions

The results exhibited that circ_0004904 expression was hampered in si-circ_0004904 group than in si-NC group (Figure 2a). Downregulated circ_0004904 encouraged cell proliferation in HTR-8/SVneo cells (Figure 2b and c). Moreover, silencing circ_0004904 expedited cell migration and invasion in HTR-8/SVneo cells (Figure 2d and e). Furthermore, cell proliferation, migration, and invasion -related proteins were detected. Circ_0004904 deficiency elevated the contents of cyclin E, vimentin, and MMP2 (Figure 2f). On the contrary, knockdown of circ_0004904 constrained cell apoptosis (Figure 2g). After that, the apoptosis-related proteins were measured, and the outcomes revealed that si-circ_0004904 transfection curbed the Caspase-3 activity and Bax levels, but elevated Bcl-2 expression (Figure 2h and i). These data exemplified that si-circ_0004904 was connected with the modulation of PE.

Figure 2 Circ_0004904 knockdown promoted HTR-8/SVneo cells development. HTR-8/SVneo cells were transfected with si-circ_0004904 or si-NC. (a) Knockdown competence of si-circ_0004904 was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.
Figure 2

Circ_0004904 knockdown promoted HTR-8/SVneo cells development. HTR-8/SVneo cells were transfected with si-circ_0004904 or si-NC. (a) Knockdown competence of si-circ_0004904 was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.

3.3 miR-19b-3p was targeted by circ_0004904

The circ_0004904 included the bond sequences of miR-19b-3p, implying that miR-19b-3p might be a target of circ_0004904 (Figure 3a). Furthermore, with respect to the normal tissues, miR-19b-3p was decreased in PE placental tissues (Figure 3b). Subsequently, the luciferase activity of circ_0004904 WT in HTR-8/SVneo cells was lessened by miR-19b-3p, but there was no alteration in the circ_0004904 MUT group (Figure 3c). In addition, the targeted relationship of miR-19b-3p and circ_0004904 was also indicated by RNA pull down assay (Figure 3d). Collectively, these results proved that circ_0004904 repressed miR-19b-3p level by directly binding to miR-19b-3p.

Figure 3 Interaction between miR-19b-3p and circ_0004904. (a) The complementary sites between miR-19b-3p and circ_0004904. (b) Relative levels of miR-19b-3p in PE tissues and normal tissues were detected. (c) HTR-8/SVneo cells were co-transfected with circ_0004904 WT or circ_0004904 MUT and miR-NC or miR-19b-3p, and the luciferase activity was detected. (d) Relative level of circ_0004904 was quantified by RNA pull down assay in HTR-8/SVneo cells. ***, P < 0.001.
Figure 3

Interaction between miR-19b-3p and circ_0004904. (a) The complementary sites between miR-19b-3p and circ_0004904. (b) Relative levels of miR-19b-3p in PE tissues and normal tissues were detected. (c) HTR-8/SVneo cells were co-transfected with circ_0004904 WT or circ_0004904 MUT and miR-NC or miR-19b-3p, and the luciferase activity was detected. (d) Relative level of circ_0004904 was quantified by RNA pull down assay in HTR-8/SVneo cells. ***, P < 0.001.

3.4 The influence of circ_0004904 deficiency on cell functions was abrogated by miR-19b-3p inhibitor in trophoblast cells

The data unveiled that miR-19b-3p level was hampered in anti-miR-19b-3p group than in anti-NC group (Figure 4a). Then, the cell behaviors were detected. The data showed that reintroduction of anti-miR-19b-3p overturned the acceleration effect of circ_0004904 deficiency on cell proliferation in HTR-8/SVneo cells (Figure 4b and c). Moreover, anti-miR-19b-3p co-transfection abolished the promoting impacts of circ_0004904 knockdown on cell migration and invasion in HTR-8/SVneo cells (Figure 4d and e). Furthermore, downregulation of circ_0004904 elevated the contents of cyclin E, vimentin, and MMP2, while anti-miR-19b-3p could relieve the influence (Figure 4f). Additionally, knockdown of circ_0004904 constrained cell apoptosis; however, this impact was neutralized by anti-miR-19b-3p in HTR-8/SVneo cells (Figure 4g). Finally, anti-miR-19b-3p transfection counteracted the impact of si-circ_0004904 on the Caspase-3 activity, Bax, and Bcl-2 levels (Figure 4h and i). These outcomes disclosed that miR-19b-3p has a vital role in circ_0004904 deficiency-induced regulation in trophoblast cells.

Figure 4 Circ_0004904 knockdown promoted HTR-8/SVneo cells development by regulating miR-19b-3p. (a) Knockdown competence of anti-miR-19b-3p was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.
Figure 4

Circ_0004904 knockdown promoted HTR-8/SVneo cells development by regulating miR-19b-3p. (a) Knockdown competence of anti-miR-19b-3p was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.

3.5 ARRDC3 was a target of miR-19b-3p

As presented in Figure 5a, starBase forecasted that ARRDC3 might be targeted by miR-19b-3p. Next relative to the normal tissues, ARRDC3 was enhanced in PE placental tissues (Figure 5b). Afterwards, the luciferase activity of ARRDC3 3′UTR WT in HTR-8/SVneo cells was lessened by miR-19b-3p, but there was no alteration in the ARRDC3 3′UTR MUT group (Figure 5c). Besides, anti-miR-19b-3p could enhance ARRDC3 content in HTR-8/SVneo cells (Figure 5d). Meanwhile, the ARRDC3 level was harmed by circ_0004904 deficiency, but elevated by anti-miR-19b-3p transfection (Figure 5e). These statistics disclosed that ARRDC3 was straightly targeted by miR-19b-3p in trophoblast cells.

Figure 5 Interaction between miR-19b-3p and ARRDC3. (a) The complementary sites between miR-19b-3p and ARRDC3. (b) Relative levels of ARRDC3 in PE tissues and normal tissues were detected. (c) HTR-8/SVneo cells were co-transfected with ARRDC3 3′UTR WT or ARRDC3 3′UTR MUT and miR-NC or miR-19b-3p, and the luciferase activity was detected. (d and e) Relative levels of ARRDC3 in PE cells were quantified. ***, P < 0.001.
Figure 5

Interaction between miR-19b-3p and ARRDC3. (a) The complementary sites between miR-19b-3p and ARRDC3. (b) Relative levels of ARRDC3 in PE tissues and normal tissues were detected. (c) HTR-8/SVneo cells were co-transfected with ARRDC3 3′UTR WT or ARRDC3 3′UTR MUT and miR-NC or miR-19b-3p, and the luciferase activity was detected. (d and e) Relative levels of ARRDC3 in PE cells were quantified. ***, P < 0.001.

3.6 Circ_0004904 regulated the trophoblast cell behaviors via ARRDC3

The records exposed that ARRDC3 was upregulated in pcDNA3.0-ARRDC3 group than in pcDNA3.0-NC group (Figure 6a). The co-transfection of pcDNA3.0-ARRDC3 reversed the acceleration effect of circ_0004904 silencing on cell proliferation in HTR-8/SVneo cells (Figure 6b and c). Moreover, ARRDC3 overexpression abolished the elevation impacts of si-circ_0004904 on cell migration and invasion in HTR-8/SVneo cells (Figure 6d and e). Furthermore, downregulation of circ_0004904 elevated the contents of cyclin E, vimentin, and MMP2, while pcDNA3.0-ARRDC3 could relieve this influence (Figure 6f). In addition, silencing of circ_0004904 inhibited cell apoptosis, but this clogged impact was neutralized by ARRDC3 in HTR-8/SVneo cells (Figure 6g). Finally, ARRDC3 overexpression lessened the impact of si-circ_0004904 on the Caspase-3 activity, Bax, and Bcl-2 levels (Figure 6h and i). Furthermore, Figure 7 exhibited the regulation mechanism of circ_0004904/miR-19b-3p/ARRDC3 axis.

Figure 6 Circ_0004904 knockdown promoted HTR-8/SVneo cells development by regulating ARRDC3. (a) Upregulation competence of pcDNA3.0-ARRDC3 was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.
Figure 6

Circ_0004904 knockdown promoted HTR-8/SVneo cells development by regulating ARRDC3. (a) Upregulation competence of pcDNA3.0-ARRDC3 was appraised by qRT-PCR. (b and c) MTT and EdU assays were enforced to evaluate cell proliferation. (d and e) Transwell assay was applied to assess cell migration and invasion. (f) Western blot assay was utilized to scrutinize the cyclin E, vimentin, and MMP2 contents. (g) Flow cytometry was conducted to assess the cell apoptosis. (h) The Caspase-3 activity was detected. (i) Western blot assay was utilized to scrutinize the Bax and Bcl2 contents. ***, P < 0.001.

Figure 7 The regulation mechanism of circ_0004904/miR-19b-3p/ARRDC3.
Figure 7

The regulation mechanism of circ_0004904/miR-19b-3p/ARRDC3.

4 Discussion

According to the current results of clinical studies, it is not difficult to find that the treatment of PE is more difficult than prevention [25]. The symptoms of PE do not stop completely pathologically. Therefore, the current treatment approach is mainly aimed to prolong pregnancy by slowing down the pathological process [26,27]. In recent years, noninvasive methods, such as nucleic acids detection, have been proposed and developed for the early diagnosis of PE due to the release of molecules of the placental compartment [28,29]. Hence, we aimed to investigate the new molecules for PE treatment.

In accordance with this work, the circ_0004904 levels in PE tissues were remarkably elevated, which was similar to that reported by Jiang et al. [9]. These records speculated that circ_0004904 might play a vital role in PE. In this work, loss of function technique was applied to assess the underlying effects of circ_0004904 on trophoblast cells. The outcomes showed that circ_0004904 downregulation contributed to cell proliferation, migration, and invasion, but restrained cell apoptosis in trophoblast cells.

CircRNAs could act as miRNA sponge to control their biological role [13]. Previous studies have revealed that altered expression of some miRNAs plays important role in the onset and development of placental-induced diseases, such as fetal growth restriction [30] and PE [31]. Here the data displayed that circ_0004904 included the target sites of miR-19b-3p. Li et al. uncovered that miR-19b-3p inhibited osteoarthritis development [32]. Moreover, miR-19b-3p could regulate cell proliferation in human pancreatic cancer [33]. In this work, it was found that miR-19b-3p levels in PE tissues were remarkably restrained, which was similar to that described by Sandrim et al. [17]. Meanwhile, miR-19b-3p could target ARRDC3 to hamper PE development. Therefore, it was suggested that circ_0004904 exercised its function by the miR-19b-3p/ARRDC3 axis.

ARRDC3 has been shown to be involved in regulating the progression of many cancers, like colorectal cancer and breast cancer [19,20]. The information in this work presented that ARRDC3 level was abnormally intensified in PE, which was alike to Lei et al. description [21]. Moreover, ARRDC3 could promote trophoblast cell dysfunction.

In short, we presented that circ_0004904 acted as a sponge of miR-19b-3p to positively adjust ARRDC3, thus accelerating PE progression. These conclusions delivered a fresh train of thought for PE handling.

# Chenyuan Cao and Jie Cui contribute to this work equally as co-first authors.

tel: +86-0312-5981818

Acknowledgement

Not applicable.

  1. Funding information: No funding was received.

  2. Author contributions: conceptualization and methodology: Jie Cui and Guiling Liu; formal analysis and data curation: Chenyuan Cao and Jie Cui; validation and investigation: Chenyuan Cao and Guiling Liu; writing – original draft preparation and writing – review and editing: Chenyuan Cao, Jie Cui, and Guiling Liu; Approval of final manuscript: all authors.

  3. Conflict of interest: The authors declare that they have no competing interests.

  4. Data availability statements: The analyzed datasets generated during the present study are available from the corresponding author on reasonable request.

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Received: 2022-02-22
Revised: 2022-06-16
Accepted: 2022-07-25
Published Online: 2023-05-16

© 2023 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Artikel in diesem Heft

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  242. Clinicopathological characteristics and diagnosis of hepatic sinusoidal obstruction syndrome caused by Tusanqi – Case report and literature review
  243. Synchronous triple primary gastrointestinal malignant tumors treated with laparoscopic surgery: A case report
  244. CT-guided percutaneous microwave ablation combined with bone cement injection for the treatment of transverse metastases: A case report
  245. Malignant hyperthermia: Report on a successful rescue of a case with the highest temperature of 44.2°C
  246. Anesthetic management of fetal pulmonary valvuloplasty: A case report
  247. Rapid Communication
  248. Impact of COVID-19 lockdown on glycemic levels during pregnancy: A retrospective analysis
  249. Erratum
  250. Erratum to “Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway”
  251. Erratum to: “Fer exacerbates renal fibrosis and can be targeted by miR-29c-3p”
  252. Retraction
  253. Retraction of “Study to compare the effect of casirivimab and imdevimab, remdesivir, and favipiravir on progression and multi-organ function of hospitalized COVID-19 patients”
  254. Retraction of “circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis”
  255. Retraction of “miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells”
  256. Retraction of “SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis”
  257. Retraction of “circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury”
  258. Retraction of “lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells”
  259. Special issue Linking Pathobiological Mechanisms to Clinical Application for cardiovascular diseases
  260. Effect of cardiac rehabilitation therapy on depressed patients with cardiac insufficiency after cardiac surgery
  261. Special issue The evolving saga of RNAs from bench to bedside - Part I
  262. FBLIM1 mRNA is a novel prognostic biomarker and is associated with immune infiltrates in glioma
  263. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part III
  264. Development of a machine learning-based signature utilizing inflammatory response genes for predicting prognosis and immune microenvironment in ovarian cancer
https://doi.org/10.1515/med-2022-0546
Schlagwörter für diesen Artikel
preeclampsia; circ_0004904; miR-19b-3p; ARRDC3
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Research Articles
  2. Exosomes derived from mesenchymal stem cells overexpressing miR-210 inhibits neuronal inflammation and contribute to neurite outgrowth through modulating microglia polarization
  3. Current situation of acute ST-segment elevation myocardial infarction in a county hospital chest pain center during an epidemic of novel coronavirus pneumonia
  4. circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway
  5. circRNA ITGA7 restrains growth and enhances radiosensitivity by up-regulating SMAD4 in colorectal carcinoma
  6. WDR79 promotes aerobic glycolysis of pancreatic ductal adenocarcinoma (PDAC) by the suppression of SIRT4
  7. Up-regulation of collagen type V alpha 2 (COL5A2) promotes malignant phenotypes in gastric cancer cell via inducing epithelial–mesenchymal transition (EMT)
  8. Inhibition of TERC inhibits neural apoptosis and inflammation in spinal cord injury through Akt activation and p-38 inhibition via the miR-34a-5p/XBP-1 axis
  9. 3D-printed polyether-ether-ketone/n-TiO2 composite enhances the cytocompatibility and osteogenic differentiation of MC3T3-E1 cells by downregulating miR-154-5p
  10. Propofol-mediated circ_0000735 downregulation restrains tumor growth by decreasing integrin-β1 expression in non-small cell lung cancer
  11. PVT1/miR-16/CCND1 axis regulates gastric cancer progression
  12. Silencing of circ_002136 sensitizes gastric cancer to paclitaxel by targeting the miR-16-5p/HMGA1 axis
  13. Short-term outcomes after simultaneous gastrectomy plus cholecystectomy in gastric cancer: A pooling up analysis
  14. SCARA5 inhibits oral squamous cell carcinoma via inactivating the STAT3 and PI3K/AKT signaling pathways
  15. Molecular mechanism by which the Notch signaling pathway regulates autophagy in a rat model of pulmonary fibrosis in pigeon breeder’s lung
  16. lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis
  17. SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosis
  18. Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation
  19. Treatments for brain metastases from EGFR/ALK-negative/unselected NSCLC: A network meta-analysis
  20. Association of osteoporosis and skeletal muscle loss with serum type I collagen carboxyl-terminal peptide β glypeptide: A cross-sectional study in elder Chinese population
  21. circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway
  22. Delivery in a vertical birth chair supported by freedom of movement during labor: A randomized control trial
  23. UBE2J1 knockdown promotes cell apoptosis in endometrial cancer via regulating PI3K/AKT and MDM2/p53 signaling
  24. Metabolic resuscitation therapy in critically ill patients with sepsis and septic shock: A pilot prospective randomized controlled trial
  25. Lycopene ameliorates locomotor activity and urinary frequency induced by pelvic venous congestion in rats
  26. UHRF1-induced connexin26 methylation is involved in hearing damage triggered by intermittent hypoxia in neonatal rats
  27. LINC00511 promotes melanoma progression by targeting miR-610/NUCB2
  28. Ultra-high-performance liquid chromatography-tandem mass spectrometry analysis of serum metabolomic characteristics in people with different vitamin D levels
  29. Role of Jumonji domain-containing protein D3 and its inhibitor GSK-J4 in Hashimoto’s thyroiditis
  30. circ_0014736 induces GPR4 to regulate the biological behaviors of human placental trophoblast cells through miR-942-5p in preeclampsia
  31. Monitoring of sirolimus in the whole blood samples from pediatric patients with lymphatic anomalies
  32. Effects of osteogenic growth peptide C-terminal pentapeptide and its analogue on bone remodeling in an osteoporosis rat model
  33. A novel autophagy-related long non-coding RNAs signature predicting progression-free interval and I-131 therapy benefits in papillary thyroid carcinoma
  34. WGCNA-based identification of potential targets and pathways in response to treatment in locally advanced breast cancer patients
  35. Radiomics model using preoperative computed tomography angiography images to differentiate new from old emboli of acute lower limb arterial embolism
  36. Dysregulated lncRNAs are involved in the progress of myocardial infarction by constructing regulatory networks
  37. Single-arm trial to evaluate the efficacy and safety of baclofen in treatment of intractable hiccup caused by malignant tumor chemotherapy
  38. Genetic polymorphisms of MRPS30-DT and NINJ2 may influence lung cancer risk
  39. Efficacy of immune checkpoint inhibitors in patients with KRAS-mutant advanced non-small cell lung cancer: A retrospective analysis
  40. Pyroptosis-based risk score predicts prognosis and drug sensitivity in lung adenocarcinoma
  41. Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis
  42. CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p
  43. Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9
  44. Downregulation of RBM17 enhances cisplatin sensitivity and inhibits cell invasion in human hypopharyngeal cancer cells
  45. HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway
  46. The association between sleep duration, quality, and nonalcoholic fatty liver disease: A cross-sectional study
  47. Myostatin silencing inhibits podocyte apoptosis in membranous nephropathy through Smad3/PKA/NOX4 signaling pathway
  48. A novel long noncoding RNA AC125257.1 facilitates colorectal cancer progression by targeting miR-133a-3p/CASC5 axis
  49. Impact of omicron wave and associated control measures in Shanghai on health management and psychosocial well-being of patients with chronic conditions
  50. Clinicopathological characteristics and prognosis of young patients aged ≤45 years old with non-small cell lung cancer
  51. TMT-based comprehensive proteomic profiling identifies serum prognostic signatures of acute myeloid leukemia
  52. The dose limits of teeth protection for patients with nasopharyngeal carcinoma undergoing radiotherapy based on the early oral health-related quality of life
  53. miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
  54. Long non-coding RNA AL137789.1 promoted malignant biological behaviors and immune escape of pancreatic carcinoma cells
  55. IRF6 and FGF1 polymorphisms in non-syndromic cleft lip with or without cleft palate in the Polish population
  56. Comprehensive analysis of the role of SFXN family in breast cancer
  57. Efficacy of bronchoscopic intratumoral injection of endostar and cisplatin in lung squamous cell carcinoma patients underwent conventional chemoradiotherapy
  58. Silencing of long noncoding RNA MIAT inhibits the viability and proliferation of breast cancer cells by promoting miR-378a-5p expression
  59. AG1024, an IGF-1 receptor inhibitor, ameliorates renal injury in rats with diabetic nephropathy via the SOCS/JAK2/STAT pathway
  60. Downregulation of KIAA1199 alleviated the activation, proliferation, and migration of hepatic stellate cells by the inhibition of epithelial–mesenchymal transition
  61. Exendin-4 regulates the MAPK and WNT signaling pathways to alleviate the osteogenic inhibition of periodontal ligament stem cells in a high glucose environment
  62. Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3
  63. The function of lncRNA EMX2OS/miR-653-5p and its regulatory mechanism in lung adenocarcinoma
  64. Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6
  65. Ultrasound examination supporting CT or MRI in the evaluation of cervical lymphadenopathy in patients with irradiation-treated head and neck cancer
  66. F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway
  67. Knockdown of circ_0005615 enhances the radiosensitivity of colorectal cancer by regulating the miR-665/NOTCH1 axis
  68. Long noncoding RNA Mhrt alleviates angiotensin II-induced cardiac hypertrophy phenotypes by mediating the miR-765/Wnt family member 7B pathway
  69. Effect of miR-499-5p/SOX6 axis on atrial fibrosis in rats with atrial fibrillation
  70. Cholesterol induces inflammation and reduces glucose utilization
  71. circ_0004904 regulates the trophoblast cell in preeclampsia via miR-19b-3p/ARRDC3 axis
  72. NECAB3 promotes the migration and invasion of liver cancer cells through HIF-1α/RIT1 signaling pathway
  73. The poor performance of cardiovascular risk scores in identifying patients with idiopathic inflammatory myopathies at high cardiovascular risk
  74. miR-2053 inhibits the growth of ovarian cancer cells by downregulating SOX4
  75. Nucleophosmin 1 associating with engulfment and cell motility protein 1 regulates hepatocellular carcinoma cell chemotaxis and metastasis
  76. α-Hederin regulates macrophage polarization to relieve sepsis-induced lung and liver injuries in mice
  77. Changes of microbiota level in urinary tract infections: A meta-analysis
  78. Identification of key enzalutamide-resistance-related genes in castration-resistant prostate cancer and verification of RAD51 functions
  79. Falls during oxaliplatin-based chemotherapy for gastrointestinal malignancies – (lessons learned from) a prospective study
  80. Outcomes of low-risk birth care during the Covid-19 pandemic: A cohort study from a tertiary care center in Lithuania
  81. Vitamin D protects intestines from liver cirrhosis-induced inflammation and oxidative stress by inhibiting the TLR4/MyD88/NF-κB signaling pathway
  82. Integrated transcriptome analysis identifies APPL1/RPS6KB2/GALK1 as immune-related metastasis factors in breast cancer
  83. Genomic analysis of immunogenic cell death-related subtypes for predicting prognosis and immunotherapy outcomes in glioblastoma multiforme
  84. Circular RNA Circ_0038467 promotes the maturation of miRNA-203 to increase lipopolysaccharide-induced apoptosis of chondrocytes
  85. An economic evaluation of fine-needle cytology as the primary diagnostic tool in the diagnosis of lymphadenopathy
  86. Midazolam impedes lung carcinoma cell proliferation and migration via EGFR/MEK/ERK signaling pathway
  87. Network pharmacology combined with molecular docking and experimental validation to reveal the pharmacological mechanism of naringin against renal fibrosis
  88. PTPN12 down-regulated by miR-146b-3p gene affects the malignant progression of laryngeal squamous cell carcinoma
  89. miR-141-3p accelerates ovarian cancer progression and promotes M2-like macrophage polarization by targeting the Keap1-Nrf2 pathway
  90. lncRNA OIP5-AS1 attenuates the osteoarthritis progression in IL-1β-stimulated chondrocytes
  91. Overexpression of LINC00607 inhibits cell growth and aggressiveness by regulating the miR-1289/EFNA5 axis in non-small-cell lung cancer
  92. Subjective well-being in informal caregivers during the COVID-19 pandemic
  93. Nrf2 protects against myocardial ischemia-reperfusion injury in diabetic rats by inhibiting Drp1-mediated mitochondrial fission
  94. Unfolded protein response inhibits KAT2B/MLKL-mediated necroptosis of hepatocytes by promoting BMI1 level to ubiquitinate KAT2B
  95. Bladder cancer screening: The new selection and prediction model
  96. circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis
  97. Prone position effect in intensive care patients with SARS-COV-2 pneumonia
  98. Clinical observation on the efficacy of Tongdu Tuina manipulation in the treatment of primary enuresis in children
  99. Dihydroartemisinin ameliorates cerebral I/R injury in rats via regulating VWF and autophagy-mediated SIRT1/FOXO1 pathway
  100. Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway
  101. Low Ang-(1–7) and high des-Arg9 bradykinin serum levels are correlated with cardiovascular risk factors in patients with COVID-19
  102. Effect of maternal age and body mass index on induction of labor with oral misoprostol for premature rupture of membrane at term: A retrospective cross-sectional study
  103. Potential protective effects of Huanglian Jiedu Decoction against COVID-19-associated acute kidney injury: A network-based pharmacological and molecular docking study
  104. Clinical significance of serum MBD3 detection in girls with central precocious puberty
  105. Clinical features of varicella-zoster virus caused neurological diseases detected by metagenomic next-generation sequencing
  106. Collagen treatment of complex anorectal fistula: 3 years follow-up
  107. LncRNA CASC15 inhibition relieves renal fibrosis in diabetic nephropathy through down-regulating SP-A by sponging to miR-424
  108. Efficacy analysis of empirical bismuth quadruple therapy, high-dose dual therapy, and resistance gene-based triple therapy as a first-line Helicobacter pylori eradication regimen – An open-label, randomized trial
  109. SMOC2 plays a role in heart failure via regulating TGF-β1/Smad3 pathway-mediated autophagy
  110. A prospective cohort study of the impact of chronic disease on fall injuries in middle-aged and older adults
  111. circRNA THBS1 silencing inhibits the malignant biological behavior of cervical cancer cells via the regulation of miR-543/HMGB2 axis
  112. hsa_circ_0000285 sponging miR-582-3p promotes neuroblastoma progression by regulating the Wnt/β-catenin signaling pathway
  113. Long non-coding RNA GNAS-AS1 knockdown inhibits proliferation and epithelial–mesenchymal transition of lung adenocarcinoma cells via the microRNA-433-3p/Rab3A axis
  114. lncRNA UCA1 regulates miR-132/Lrrfip1 axis to promote vascular smooth muscle cell proliferation
  115. Twenty-four-color full spectrum flow cytometry panel for minimal residual disease detection in acute myeloid leukemia
  116. Hsa-miR-223-3p participates in the process of anthracycline-induced cardiomyocyte damage by regulating NFIA gene
  117. Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice
  118. Analysis of somatic mutations and key driving factors of cervical cancer progression
  119. Hsa_circ_0028007 regulates the progression of nasopharyngeal carcinoma through the miR-1179/SQLE axis
  120. Variations in sexual function after laparoendoscopic single-site hysterectomy in women with benign gynecologic diseases
  121. Effects of pharmacological delay with roxadustat on multi-territory perforator flap survival in rats
  122. Analysis of heroin effects on calcium channels in rat cardiomyocytes based on transcriptomics and metabolomics
  123. Risk factors of recurrent bacterial vaginosis among women of reproductive age: A cross-sectional study
  124. Alkbh5 plays indispensable roles in maintaining self-renewal of hematopoietic stem cells
  125. Study to compare the effect of casirivimab and imdevimab, remdesivir, and favipiravir on progression and multi-organ function of hospitalized COVID-19 patients
  126. Correlation between microvessel maturity and ISUP grades assessed using contrast-enhanced transrectal ultrasonography in prostate cancer
  127. The protective effect of caffeic acid phenethyl ester in the nephrotoxicity induced by α-cypermethrin
  128. Norepinephrine alleviates cyclosporin A-induced nephrotoxicity by enhancing the expression of SFRP1
  129. Effect of RUNX1/FOXP3 axis on apoptosis of T and B lymphocytes and immunosuppression in sepsis
  130. The function of Foxp1 represses β-adrenergic receptor transcription in the occurrence and development of bladder cancer through STAT3 activity
  131. Risk model and validation of carbapenem-resistant Klebsiella pneumoniae infection in patients with cerebrovascular disease in the ICU
  132. Calycosin protects against chronic prostatitis in rats via inhibition of the p38MAPK/NF-κB pathway
  133. Pan-cancer analysis of the PDE4DIP gene with potential prognostic and immunotherapeutic values in multiple cancers including acute myeloid leukemia
  134. The safety and immunogenicity to inactivated COVID-19 vaccine in patients with hyperlipemia
  135. Circ-UBR4 regulates the proliferation, migration, inflammation, and apoptosis in ox-LDL-induced vascular smooth muscle cells via miR-515-5p/IGF2 axis
  136. Clinical characteristics of current COVID-19 rehabilitation outpatients in China
  137. Luteolin alleviates ulcerative colitis in rats via regulating immune response, oxidative stress, and metabolic profiling
  138. miR-199a-5p inhibits aortic valve calcification by targeting ATF6 and GRP78 in valve interstitial cells
  139. The application of iliac fascia space block combined with esketamine intravenous general anesthesia in PFNA surgery of the elderly: A prospective, single-center, controlled trial
  140. Elevated blood acetoacetate levels reduce major adverse cardiac and cerebrovascular events risk in acute myocardial infarction
  141. The effects of progesterone on the healing of obstetric anal sphincter damage in female rats
  142. Identification of cuproptosis-related genes for predicting the development of prostate cancer
  143. Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activity
  144. Targeting PTBP1 blocks glutamine metabolism to improve the cisplatin sensitivity of hepatocarcinoma cells through modulating the mRNA stability of glutaminase
  145. A single center prospective study: Influences of different hip flexion angles on the measurement of lumbar spine bone mineral density by dual energy X-ray absorptiometry
  146. Clinical analysis of AN69ST membrane continuous venous hemofiltration in the treatment of severe sepsis
  147. Antibiotics therapy combined with probiotics administered intravaginally for the treatment of bacterial vaginosis: A systematic review and meta-analysis
  148. Construction of a ceRNA network to reveal a vascular invasion associated prognostic model in hepatocellular carcinoma
  149. A pan-cancer analysis of STAT3 expression and genetic alterations in human tumors
  150. A prognostic signature based on seven T-cell-related cell clustering genes in bladder urothelial carcinoma
  151. Pepsin concentration in oral lavage fluid of rabbit reflux model constructed by dilating the lower esophageal sphincter
  152. The antihypertensive felodipine shows synergistic activity with immune checkpoint blockade and inhibits tumor growth via NFAT1 in LUSC
  153. Tanshinone IIA attenuates valvular interstitial cells’ calcification induced by oxidized low density lipoprotein via reducing endoplasmic reticulum stress
  154. AS-IV enhances the antitumor effects of propofol in NSCLC cells by inhibiting autophagy
  155. Establishment of two oxaliplatin-resistant gallbladder cancer cell lines and comprehensive analysis of dysregulated genes
  156. Trial protocol: Feasibility of neuromodulation with connectivity-guided intermittent theta-burst stimulation for improving cognition in multiple sclerosis
  157. LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer
  158. Factors associated with gastrointestinal dysmotility in critically ill patients
  159. Mechanisms by which spinal cord stimulation intervenes in atrial fibrillation: The involvement of the endothelin-1 and nerve growth factor/p75NTR pathways
  160. Analysis of two-gene signatures and related drugs in small-cell lung cancer by bioinformatics
  161. Silencing USP19 alleviates cigarette smoke extract-induced mitochondrial dysfunction in BEAS-2B cells by targeting FUNDC1
  162. Menstrual irregularities associated with COVID-19 vaccines among women in Saudi Arabia: A survey during 2022
  163. Ferroptosis involves in Schwann cell death in diabetic peripheral neuropathy
  164. The effect of AQP4 on tau protein aggregation in neurodegeneration and persistent neuroinflammation after cerebral microinfarcts
  165. Activation of UBEC2 by transcription factor MYBL2 affects DNA damage and promotes gastric cancer progression and cisplatin resistance
  166. Analysis of clinical characteristics in proximal and distal reflux monitoring among patients with gastroesophageal reflux disease
  167. Exosomal circ-0020887 and circ-0009590 as novel biomarkers for the diagnosis and prediction of short-term adverse cardiovascular outcomes in STEMI patients
  168. Upregulated microRNA-429 confers endometrial stromal cell dysfunction by targeting HIF1AN and regulating the HIF1A/VEGF pathway
  169. Bibliometrics and knowledge map analysis of ultrasound-guided regional anesthesia
  170. Knockdown of NUPR1 inhibits angiogenesis in lung cancer through IRE1/XBP1 and PERK/eIF2α/ATF4 signaling pathways
  171. D-dimer trends predict COVID-19 patient’s prognosis: A retrospective chart review study
  172. WTAP affects intracranial aneurysm progression by regulating m6A methylation modification
  173. Using of endoscopic polypectomy in patients with diagnosed malignant colorectal polyp – The cross-sectional clinical study
  174. Anti-S100A4 antibody administration alleviates bronchial epithelial–mesenchymal transition in asthmatic mice
  175. Prognostic evaluation of system immune-inflammatory index and prognostic nutritional index in double expressor diffuse large B-cell lymphoma
  176. Prevalence and antibiogram of bacteria causing urinary tract infection among patients with chronic kidney disease
  177. Reactive oxygen species within the vaginal space: An additional promoter of cervical intraepithelial neoplasia and uterine cervical cancer development?
  178. Identification of disulfidptosis-related genes and immune infiltration in lower-grade glioma
  179. A new technique for uterine-preserving pelvic organ prolapse surgery: Laparoscopic rectus abdominis hysteropexy for uterine prolapse by comparing with traditional techniques
  180. Self-isolation of an Italian long-term care facility during COVID-19 pandemic: A comparison study on care-related infectious episodes
  181. A comparative study on the overlapping effects of clinically applicable therapeutic interventions in patients with central nervous system damage
  182. Low intensity extracorporeal shockwave therapy for chronic pelvic pain syndrome: Long-term follow-up
  183. The diagnostic accuracy of touch imprint cytology for sentinel lymph node metastases of breast cancer: An up-to-date meta-analysis of 4,073 patients
  184. Mortality associated with Sjögren’s syndrome in the United States in the 1999–2020 period: A multiple cause-of-death study
  185. CircMMP11 as a prognostic biomarker mediates miR-361-3p/HMGB1 axis to accelerate malignant progression of hepatocellular carcinoma
  186. Analysis of the clinical characteristics and prognosis of adult de novo acute myeloid leukemia (none APL) with PTPN11 mutations
  187. KMT2A maintains stemness of gastric cancer cells through regulating Wnt/β-catenin signaling-activated transcriptional factor KLF11
  188. Evaluation of placental oxygenation by near-infrared spectroscopy in relation to ultrasound maturation grade in physiological term pregnancies
  189. The role of ultrasonographic findings for PIK3CA-mutated, hormone receptor-positive, human epidermal growth factor receptor-2-negative breast cancer
  190. Construction of immunogenic cell death-related molecular subtypes and prognostic signature in colorectal cancer
  191. Long-term prognostic value of high-sensitivity cardiac troponin-I in patients with idiopathic dilated cardiomyopathy
  192. Establishing a novel Fanconi anemia signaling pathway-associated prognostic model and tumor clustering for pediatric acute myeloid leukemia patients
  193. Integrative bioinformatics analysis reveals STAT2 as a novel biomarker of inflammation-related cardiac dysfunction in atrial fibrillation
  194. Adipose-derived stem cells repair radiation-induced chronic lung injury via inhibiting TGF-β1/Smad 3 signaling pathway
  195. Real-world practice of idiopathic pulmonary fibrosis: Results from a 2000–2016 cohort
  196. lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
  197. Diagnostic value of urinary Tamm-Horsfall protein and 24 h urine osmolality for recurrent calcium oxalate stones of the upper urinary tract: Cross-sectional study
  198. The value of color Doppler ultrasonography combined with serum tumor markers in differential diagnosis of gastric stromal tumor and gastric cancer
  199. The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A
  200. Mycophenolate mofetil versus cyclophosphamide plus in patients with connective tissue disease-associated interstitial lung disease: Efficacy and safety analysis
  201. MiR-1278 targets CALD1 and suppresses the progression of gastric cancer via the MAPK pathway
  202. Metabolomic analysis of serum short-chain fatty acid concentrations in a mouse of MPTP-induced Parkinson’s disease after dietary supplementation with branched-chain amino acids
  203. Cimifugin inhibits adipogenesis and TNF-α-induced insulin resistance in 3T3-L1 cells
  204. Predictors of gastrointestinal complaints in patients on metformin therapy
  205. Prescribing patterns in patients with chronic obstructive pulmonary disease and atrial fibrillation
  206. A retrospective analysis of the effect of latent tuberculosis infection on clinical pregnancy outcomes of in vitro fertilization–fresh embryo transferred in infertile women
  207. Appropriateness and clinical outcomes of short sustained low-efficiency dialysis: A national experience
  208. miR-29 regulates metabolism by inhibiting JNK-1 expression in non-obese patients with type 2 diabetes mellitus and NAFLD
  209. Clinical features and management of lymphoepithelial cyst
  210. Serum VEGF, high-sensitivity CRP, and cystatin-C assist in the diagnosis of type 2 diabetic retinopathy complicated with hyperuricemia
  211. ENPP1 ameliorates vascular calcification via inhibiting the osteogenic transformation of VSMCs and generating PPi
  212. Significance of monitoring the levels of thyroid hormone antibodies and glucose and lipid metabolism antibodies in patients suffer from type 2 diabetes
  213. The causal relationship between immune cells and different kidney diseases: A Mendelian randomization study
  214. Interleukin 33, soluble suppression of tumorigenicity 2, interleukin 27, and galectin 3 as predictors for outcome in patients admitted to intensive care units
  215. Identification of diagnostic immune-related gene biomarkers for predicting heart failure after acute myocardial infarction
  216. Long-term administration of probiotics prevents gastrointestinal mucosal barrier dysfunction in septic mice partly by upregulating the 5-HT degradation pathway
  217. miR-192 inhibits the activation of hepatic stellate cells by targeting Rictor
  218. Diagnostic and prognostic value of MR-pro ADM, procalcitonin, and copeptin in sepsis
  219. Review Articles
  220. Prenatal diagnosis of fetal defects and its implications on the delivery mode
  221. Electromagnetic fields exposure on fetal and childhood abnormalities: Systematic review and meta-analysis
  222. Characteristics of antibiotic resistance mechanisms and genes of Klebsiella pneumoniae
  223. Saddle pulmonary embolism in the setting of COVID-19 infection: A systematic review of case reports and case series
  224. Vitamin C and epigenetics: A short physiological overview
  225. Ebselen: A promising therapy protecting cardiomyocytes from excess iron in iron-overloaded thalassemia patients
  226. Aspirin versus LMWH for VTE prophylaxis after orthopedic surgery
  227. Mechanism of rhubarb in the treatment of hyperlipidemia: A recent review
  228. Surgical management and outcomes of traumatic global brachial plexus injury: A concise review and our center approach
  229. The progress of autoimmune hepatitis research and future challenges
  230. METTL16 in human diseases: What should we do next?
  231. New insights into the prevention of ureteral stents encrustation
  232. VISTA as a prospective immune checkpoint in gynecological malignant tumors: A review of the literature
  233. Case Reports
  234. Mycobacterium xenopi infection of the kidney and lymph nodes: A case report
  235. Genetic mutation of SLC6A20 (c.1072T > C) in a family with nephrolithiasis: A case report
  236. Chronic hepatitis B complicated with secondary hemochromatosis was cured clinically: A case report
  237. Liver abscess complicated with multiple organ invasive infection caused by hematogenous disseminated hypervirulent Klebsiella pneumoniae: A case report
  238. Urokinase-based lock solutions for catheter salvage: A case of an upcoming kidney transplant recipient
  239. Two case reports of maturity-onset diabetes of the young type 3 caused by the hepatocyte nuclear factor 1α gene mutation
  240. Immune checkpoint inhibitor-related pancreatitis: What is known and what is not
  241. Does total hip arthroplasty result in intercostal nerve injury? A case report and literature review
  242. Clinicopathological characteristics and diagnosis of hepatic sinusoidal obstruction syndrome caused by Tusanqi – Case report and literature review
  243. Synchronous triple primary gastrointestinal malignant tumors treated with laparoscopic surgery: A case report
  244. CT-guided percutaneous microwave ablation combined with bone cement injection for the treatment of transverse metastases: A case report
  245. Malignant hyperthermia: Report on a successful rescue of a case with the highest temperature of 44.2°C
  246. Anesthetic management of fetal pulmonary valvuloplasty: A case report
  247. Rapid Communication
  248. Impact of COVID-19 lockdown on glycemic levels during pregnancy: A retrospective analysis
  249. Erratum
  250. Erratum to “Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway”
  251. Erratum to: “Fer exacerbates renal fibrosis and can be targeted by miR-29c-3p”
  252. Retraction
  253. Retraction of “Study to compare the effect of casirivimab and imdevimab, remdesivir, and favipiravir on progression and multi-organ function of hospitalized COVID-19 patients”
  254. Retraction of “circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis”
  255. Retraction of “miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells”
  256. Retraction of “SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis”
  257. Retraction of “circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury”
  258. Retraction of “lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells”
  259. Special issue Linking Pathobiological Mechanisms to Clinical Application for cardiovascular diseases
  260. Effect of cardiac rehabilitation therapy on depressed patients with cardiac insufficiency after cardiac surgery
  261. Special issue The evolving saga of RNAs from bench to bedside - Part I
  262. FBLIM1 mRNA is a novel prognostic biomarker and is associated with immune infiltrates in glioma
  263. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part III
  264. Development of a machine learning-based signature utilizing inflammatory response genes for predicting prognosis and immune microenvironment in ovarian cancer
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Heruntergeladen am 17.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/med-2022-0546/html
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