Analysis of serum metabolomics in patients with different types of chronic heart failure
-
Bingzhang Jie
,
Qiang Li
Abstract
Background
Heart failure remains a major public health issue, and there are still no reliable biomarkers for left ventricular ejection fraction (LVEF).
Objective
To screen for differential metabolites in the blood of HFpEF, HFmrEF, and HFrEF patients based on metabolomics analysis of their blood samples.
Methods
Total 44 patients in HFpEF group, 30 patients in HFmrEF group, and 36 patients in HFrEF group were selected. The blood metabolites were analyzed by liquid chromatography high-resolution mass spectrometry and classified by principal component analysis, and then potential biomarker were screened. Partial least squares discriminant analysis was used to model and investigate the predictive ability of biomarkers for LVEF.
Results
Blood metabolite profiles of HFpEF, HFmrEF, and HFrEF groups could be well distinguished, and seven potential biomarkers were identified, such as phosphatidylcholine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylcholine, ceramide, sphingosine, and sphingomyelin. Four metabolic pathways, such as glycerol phospholipid metabolic pathway, linoleic acid metabolic pathway, purine pyrimidine metabolism pathway, and linolenic acid metabolism pathway were identified, among which glycerol phospholipid metabolism pathway was the most significant.
Conclusion
The changes in glycerol phospholipid metabolism pathway may help identify HFpEF, HFmrEF, and HFrEF.
1 Introduction
Heart failure (HF) is a complex syndrome that is the final stage of various cardiovascular diseases. According to left ventricular ejection fraction (LVEF), HF can be divided into HF with preserved ejection fraction (HFpEF, LVEF ≥ 50%), HF with mildly reduced ejection fraction (HFmrEF, LVEF 41–49%), and HF with reduced ejection fraction (HFrEF, LVEF ≤ 40%). Despite significant progress in the diagnosis and treatment of HF, patients still have severe prognosis. Therefore, there is an urgent need to explore new biomarkers for HF.
The occurrence and development of HF involve multiple pathophysiological pathways such as metabolism, neurohormones, and the immune system. At present, many biomarker related to HF have been identified, such as brain natriuretic peptide (BNP), tumor factor TNF, interleukins IL-1β, IL-6, IL-18, IL-33, and Stromelysin-2 [1,2]. However, no laboratory indicators related to LVEF have been found. While BNP may be elevated in HF patients with preserved LVEF, it cannot distinguish HFpEF, HFmrEF, and HFrEF patients. The metabolic disorders in patients with HF are significant, and metabolomics help us understand the metabolic disorders associated with myocardial dysfunction.
Metabolomics refers to the use of systematic methods such as nuclear magnetic resonance, high-performance liquid chromatography, gas chromatography, and mass spectrometry to analyze the changes in endogenous metabolic product profiles in biological fluids and tissues caused by pathological physiological stimuli and genetic modifications. Among them, liquid chromatography-mass spectrometry technology has higher sensitivity and resolution, making it suitable for metabolomics research [3]. Despite many previous studies on the metabolomics of HF, we have very little knowledge on the differences in metabolomics among the patients with HFpEF, HFmrEF, and HFrEF.
Therefore, in this study we aimed to investigate characteristic metabolic spectrum of HF. We employed liquid chromatography-high-resolution mass spectrometry to obtain metabolite profiles of blood samples from patients with HFpEF, HFmrEF, and HFrEF, and conducted bioinformatics analysis of differential metabolites. Representative biomarkers were screened to provide new diagnostic strategies for HF.
2 Methods
2.1 Instruments and reagents
Following instruments and reagents were used in this study: Waters liquid chromatograph (Watsch, USA); Waters SYNAPT G2-Si mass spectrometer (Watsch, USA); high-speed freezing centrifuge (Sigma, USA), vacuum concentrator (Labconco, USA); methanol, water, acetonitrile, formic acid, and ammonium formate (mass spectrometry pure, Thermo Fisher, USA); and HSS T3 column (2.1 × 100 mm, 1.8 µm) (Watsch, USA).
2.2 Subjects
Blood samples were collected from HF patients at Fuxing Hospital affiliated with Capital Medical University, who were selected from 110 patients with congestive HF admitted to the Cardiovascular Department from September 2018 to December 2020. Inclusion criteria included: (1) all met the diagnostic criteria for central HF in the 2018 Chinese Heart Failure Diagnosis and Treatment Guidelines. (2) According to the 2021 ESC guidelines, HF was divided into HF with preserved ejection fraction (HFpEF, LVEF ≥ 50%), HF with mildly reduced ejection fraction (HFmrEF, LVEF 41%–49%), and HF with reduced ejection fraction (HFrEF, LVEF ≤ 40%), with 44 cases in the HFpEF group, 30 cases in the HFmrEF group, and 36 cases in the HFrEF group.
Exclusion criteria were (1) patients admitted without the main cause of HF; (2) those who die in the hospital; (3) patients with chronic HF caused by other reasons (such as blood system, chronic anemic heart disease, endocrine and metabolic factors such as hyperthyroidism heart disease), except for coronary heart disease, high heart disease, and dilated heart disease; (4) congenital heart disease patients with severe complications that require surgical treatment (such as valve disease); (5) patients with liver and kidney failure; (6) those who suffer from other serious systemic diseases such as malignant tumors, hemorrhagic diseases, severe acute infections or metabolic disorders; and (7) those who cannot clearly and accurately describe their medical history due to consciousness disorders, intellectual disabilities, or vague language. The general information of the samples is shown in Table 1. Blood samples were collected immediately upon admission, and centrifuged at 3,000 rpm for 10 min at 4°C. The supernatant was stored at −80°C for future use.
Baseline characteristics of study patients in temperature groups
| Characteristic | LVEF ≥ 50% | LVEF 41–49% | LVEF ≤ 40% | p-value |
|---|---|---|---|---|
| Number (n) | 44 | 30 | 36 | |
| Age (years) | 81.3 ± 9.9 | 77.0 ± 11.8 | 74.4 ± 15.6 | 0.100 |
| Gender (male) | 18 (40.9%) | 18 (60.0%) | 24 (66.7%) | 0.055 |
| Weight (kg) | 65.9 ± 8.7 | 66.1 ± 9.3 | 67.7 ± 10.8 | 0.678 |
| BMI (kg/m2) | 24.5 ± 2.7 | 23.8 ± 2.8 | 24.1 ± 2.8 | 0.199 |
| Heart rate (beats/min) | 81.5 ± 17.1 | 81.7 ± 18.0 | 88.6 ± 22.1 | 0.130 |
| Systolic pressure (mmHg) | 140.3 ± 28.4 | 144.4 ± 24.2 | 131 ± 24.2 | 0.100 |
| LVEF (%) | 57.4 ± 3.9 | 45.5 ± 2.5 | 31.1 ± 5.9 | <0.001 |
| LVIDd (mm) | 45.0 ± 6.3 | 50.3 ± 7.1 | 56.5 ± 8.2 | <0.001 |
| TC (mmol/L) | 4.6 ± 0.7 | 4.5 ± 0.8 | 4.2 ± 0.5 | 0.873 |
| TG (mmol/L) | 1.88 ± 0.57 | 1.76 ± 0.65 | 1.54 ± 0.71 | 0.579 |
| LDL-C (mmol/L) | 2.89 ± 0.97 | 2.78 ± 0.85 | 2.54 ± 0.88 | 0.355 |
| HDL (mmol/L) | 1.36 ± 0.29 | 1.27 ± 0.38 | 1.14 ± 0.42 | 0.483 |
| BNP (pg/mL) | 1432.9 ± 1541.2 | 1963.5 ± 1468.8 | 2751.5 ± 1645.3 | <0.001 |
| Serum potassium (mmol/L) | 4.0 ± 0.9 | 3.5 ± 0.7 | 3.7 ± 0.8 | 0.009 |
| Serum sodium (mmol/L) | 133.8 ± 6.1 | 133.2 ± 5.4 | 135.1 ± 6.9 | 0.329 |
| BUN (mg/dL) | 39.3 ± 22.5 | 36.7 ± 20.9 | 40.3 ± 25.7 | 0.835 |
| Blood creatinine (μmol/L) | 146.4 ± 81.3 | 162.7 ± 179.9 | 141.2 ± 75.8 | 0.975 |
| eGFR (mL/min·1.73 m2) | 44.6 ± 23.7 | 50.9 ± 29.6 | 50.6 ± 25.2 | 0.548 |
| Blood glucose (mmol/L) | 6.8 ± 0.9 | 6.5 ± 0.7 | 6.3 ± 0.7 | 0.729 |
| Combined diseases | ||||
| Coronary heart disease | 37 (84.1%) | 28 (93.3%) | 33 (91.7%) | 0.380 |
| Hypertension | 34 (77.3%) | 22 (73.3%) | 26 (72.2%) | 0.862 |
| Diabetes | 17 (38.6%) | 10 (33.3%) | 11 (30.6%) | 0.741 |
| Hyperlipidemia | 30 (68.2%) | 19 (63.3%) | 26 (72.2%) | 0.742 |
| Atrial fibrillation/atrial flutter | 26 (59.1%) | 15 (50.0%) | 13 (36.1%) | 0.123 |
| Valvular disease | 12 (27.3%) | 9 (30.0%) | 9 (25.0%) | 0.902 |
| Medications | ||||
| β Receptor blockers | 31 (70.5%) | 23 (76.7%) | 30 (83.3%) | 0.402 |
| ACEI/ARBs | 22 (50.0%) | 18 (60.0%) | 16 (44.4%) | 0.447 |
| Spironolactone | 16 (36.4%) | 20 (66.7%) | 25 (69.4%) | 0.004 |
| Digoxin | 6 (13.6%) | 2 (6.7%) | 9 (25.0%) | 0.111 |
| Loop diuretic | 28 (63.6%) | 24 (80.0%) | 32 (88.9%) | 0.026 |
2.3 Sample processing
The samples were thawed at room temperature, and mixed with 400 μL pre-cooled mass spectrometry grade methanol solution (final concentration 80%), fully vortexed, and put on ice for 30 min. After centrifugation at 13,000 rpm for 15 min at 4°C, the supernatants (approximately 200 μL) were collected and centrifuged again at 13,000 rpm for 15 min. The supernatants were subjected to metabolomics analysis.
2.4 Metabolomics analysis
Chromatographic column: Waters Acquisition HSS T3 (2.1 × 100 mm, 1.8 µm), injection volume: 5 µL, flow rate: 0.4 mL/min, and column temperature: 40°C. Mass spectrometry conditions: capillary 1.5 kV, sample cone 30 V, desolvation gas temperature 500°C, desolvation gas flow 800 L/h, source temperature 120°C, and mobility carrier gas 32 mL/min. The ion scanning range 50–1,000m/z, and scanning time 0.2 s.
2.5 Data processing
Mass spectrometry contour map was imported into the Waters Nonlinear Progenesis (QI) software, with QC sample contour map as a reference sample alternative. The samples were divided into three groups: normal ejection fraction group, ejection fraction greater than 40% group, and ejection fraction less than 40% group. Principal component analysis (PCA) of unsupervised pattern recognition and partial least squares analysis (PLS-DA) of supervised pattern recognition were performed. Variable importance in the project was calculated based on the PLS-DA model. Differential compounds were identified based on p-value < 0.05 and fold change (Max/Min) > 1.2 values, and analyzed with Human Metabolome Database and Kyoto Encyclopedia of Genes and Genomes database. The differential metabolites were imported into MetaboAnalyst 4.0 (www.metaboanalysts.ca) for enrichment analysis, metabolic pathway analysis, and other related bioinformatics analysis.
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Informed consent: All patients provided written informed consent.
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Ethical approval: The study was approved by Ethics Committee of Fuxing Hospital and was carried out in accordance with ethical guidelines of Ethics Committee of Fuxing Hospital.
3 Results
3.1 PCA analysis of samples from different groups
As shown in Figure 1a, samples within the same group exhibited a good aggregation state, indicating good consistency in endogenous differential metabolites within the same group. There were significant differences in the contents of metabolites in different groups. The clustering circle of the group with normal ejection fraction (i.e., ejection fraction retention group) (EF Normal, EF ≥ 50%) was far from other groups, indicating significant difference in metabolites between the ejection fraction retention group and the group with mild reduction in ejection fraction (EF > 40%) and the group with reduced ejection fraction (EF < 40%). The difference in metabolites between patients with mild decrease in ejection fraction (EF > 40%) and those with reduced ejection fraction (EF < 40%) was relatively weak.
PCA of heart failure patient groups with different ejection fractions. (a) PCA chart, where black represents the group with preserved ejection fraction, blue represents the group of heart failure patients with an ejection fraction greater than 40%, and red represents the group of heart failure patients with an ejection fraction less than 40%. (b) Goodness of fit test of PCA diagram. Under the sixth principal component, the cumulative value of R 2 and Q 2 was close to one thousand, indicating good goodness of PCA analysis model.
Figure 1b shows the fitting score of PCA model. Under the sixth principal component, the cumulative value of R 2 and Q 2 was close to one thousand, indicating good goodness of PCA analysis model.
3.2 Identification and analysis of differential metabolites in different groups
The differential metabolites were identified using fragment ions from relevant databases, mainly including lipid substances such as phosphatidylcholine (PC), phosphatidylinositol (PI), lysophosphatidylcholine (LysoPC), lysophosphatidylphosphatidylcholine (LysoPE), ceramide, sphingosine, and sphingomyelin (SM).
Enrichment of metabolic pathways was carried out on three groups of differential metabolites. As shown in Figure 2a, the horizontal axis represented the important values of the metabolic pathway influence of differential metabolites, while the vertical axis represented the p-value size of the specialized metabolic pathway. The darker the color of the enriched metabolic pathway, the smaller p-value; the larger the circle, the more important the metabolic pathways affected. Under these conditions, the glycerol phospholipid metabolism pathway, linoleic acid metabolism pathway, purine pyrimidine metabolism pathway, and linolenic acid metabolism were all significantly regulated (Figure 2b).
Metabolite enrichment analysis of three sets of differential metabolites. (a) Bubble diagram of metabolic enrichment. The larger the circle, the more important the metabolic pathways affected. (b) Importance value of metabolic pathway enrichment. The horizontal axis represented the important values of the metabolic pathway influence of differential metabolites, while the vertical axis represented the p-value size of the specialized metabolic pathway.
4 Discussion
HF is a serious and complex syndrome with high incidence rate and mortality, and no reliable indicators related to LVEF have been identified. Metabolomics is an innovative tool that can be used to detect new biomarkers of diseases [4,5]. Metabolomics has shown diagnostic value in evaluating metabolic disorders related to HF, and the distribution of metabolites provides better prognostic value compared to conventional biomarkers [6–8]. Therefore, metabolomics is expected to become a favorable tool for detecting biomarkers related to LVEF.
The liquid phase high-resolution mass spectrometry method used in this study has the characteristics of high sensitivity, high resolution, and good resolution, and has been widely used in basic and clinical metabolomics research in recent years. By using this method to detect the blood samples of patients with HFpEF, HFmrEF, and HFrEF, we identified seven potential biomarkers and several metabolic pathways related to the differences among HFpEF, HFmrEF, and HFrEF.
In this study, fragment ions from relevant databases were used to identify the differential metabolites. The differential metabolites in the three groups of patients with HFrEF, HFmrEF, and HFpEF mainly included lipid substances, such as PC, PI, LysoPC, LysoPE, ceramide, sphingosine, and SM. In terms of metabolic pathways, the glycerol phospholipid metabolic pathway, linoleic acid metabolic pathway, purine pyrimidine metabolic pathway, and linolenic acid metabolism were significantly regulated in the three groups of patients with HFrEF, HFmrEF, and HFpEF, with the glycerol phospholipid metabolic pathway being particularly important.
Glycerophospholipid metabolism is important for myocardial cells. LysoPCs, lysophosphatidylethanolamine (LysoPEs), lysophosphatidylserine, lysophosphatidic acid (LysoPEs), lysophosphatidylinositol, and lysophosphatidylglycerol can be produced after the hydrolysis of the side chain of glycerol phospholipid [9]. The metabolic abnormalities of lysophospholipids in the body are closely related to many diseases. As an important component of myocardial energy metabolism, glycerol phospholipids accelerate the degradation of myocardial cell membrane phospholipids when myocardial injury occurs, and may even be accompanied by severe mitochondrial function loss [10]. Cardiolipin (CL) is also synthesized during the metabolism of glycerol phospholipids. CL is one of the main phospholipids in inner mitochondrial membrane, which is related to the assembly and activity of various protein complexes in mitochondria. The respiratory chain complexes I to V and the proteins of the solute carrier family have shown tight binding with CL. CL not only binds to the surface of these proteins, but also promotes their assembly into supercomplexes and stabilizes their structure [11]. In HF, the loss of CL and tetralinolenic CL promotes the production of excessive reactive oxygen species, which are byproducts of inefficient mitochondrial electron transport chain complexes I and III, leading to cytochrome c catalyzed CL oxidation and myocardial cell apoptosis. Therefore, protecting CL and mitochondrial function may be key to preventing the development of HF [12].
Previous studies have shown close relationship between glycerophospholipid metabolism and HF. Miao et al. identified a total of 210 metabolites with significant differences between HF rats and normal rats. These metabolites mainly participate in tryptophan metabolism, branched chain amino acid metabolism, fatty acid metabolism, β oxidation, and glycerol phospholipid metabolism [13]. Wen et al. found that therapeutic effect of HF with hydrogenated amine combined with 6-gingerol was mainly related to the regulation of acetylphosphate, 3-carboxyl-1-hydroxypropylthiamine diphosphate, coenzyme A, palmitic acid, and oleic acid. Metabolic pathway analysis showed that the pathways affecting energy metabolism included glycerol phospholipid metabolism, fatty acid metabolism, pantothenic acid, coenzyme A biosynthesis, citric acid cycle (TCA cycle), pyruvate metabolism, and arachidonic acid metabolism [14]. The Linggui Zhugan Decoction could delay the pathological process of HF by regulating the disordered metabolic pathway and related enzymes, and the most crucial pathway is the metabolism of glycerol phospholipid and arachidonic acid [15]. However, none of the above studies have explored the metabolomic differences among HFrEF, HFmrEF, and HFpEF patients. In this study, blood samples from HFpEF, HFmrEF, and HFrEF patients were analyzed by metabonomics, and we identified seven potential biomarker and four metabolic pathways, but the underlying mechanisms still need to be further explored to develop novel biomarkers for chronic HF [16].
In conclusion, we analyzed blood metabonomics of 110 patients with congestive HF who were divided into three groups of HFpEF, HFmrEF, and HFrEF. The differential metabolites among them mainly include lipid substances, such as PC, PI, LysoPC, LysoPE, ceramide, sphingosine, and SM. The glycerol phospholipid metabolism pathway, linoleic acid metabolism pathway, purine pyrimidine metabolism pathway, and linolenic acid metabolism have all undergone significant regulation, among which the glycerol phospholipid metabolism pathway is particularly important. Changes in glycerol phospholipid metabolism pathway may help identify HFpEF, HFmrEF, and HFrEF.
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Funding information: This study was supported by the scientific research and cultivation fund of Capital Medical University (No. pyz2018104).
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Author contributions: Q.L., L.H., L.C., and M.Y. collected and analyzed the data; B.J. designed and supervised the study. All authors read and approved the manuscript.
-
Conflict of interest: The authors state no conflict of interest.
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Data availability statement: All data generated or analyzed during this study are availabel upon request.
References
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This work is licensed under the Creative Commons Attribution 4.0 International License.
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- Comparison and correlations between findings of hysteroscopy and vaginal color Doppler ultrasonography for detection of uterine abnormalities in patients with recurrent implantation failure
- The effects of different types of RAGT on balance function in stroke patients with low levels of independent walking in a convalescent rehabilitation hospital
- Causal relationship between asthma and ankylosing spondylitis: A bidirectional two-sample univariable and multivariable Mendelian randomization study
- Correlations of health literacy with individuals’ understanding and use of medications in Southern Taiwan
- Correlation of serum calprotectin with outcome of acute cerebral infarction
- Comparison of computed tomography and guided bronchoscopy in the diagnosis of pulmonary nodules: A systematic review and meta-analysis
- Curdione protects vascular endothelial cells and atherosclerosis via the regulation of DNMT1-mediated ERBB4 promoter methylation
- The identification of novel missense variant in ChAT gene in a patient with gestational diabetes denotes plausible genetic association
- Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance
- Exploring the role of succinyl carnitine in the association between CD39⁺ CD4⁺ T cell and ulcerative colitis: A Mendelian randomization study
- Dexmedetomidine suppresses microglial activation in postoperative cognitive dysfunction via the mmu-miRNA-125/TRAF6 signaling axis
- Analysis of serum metabolomics in patients with different types of chronic heart failure
- Diagnostic value of hematological parameters in the early diagnosis of acute cholecystitis
- Pachymaran alleviates fat accumulation, hepatocyte degeneration, and injury in mice with nonalcoholic fatty liver disease
- Decrease in CD4 and CD8 lymphocytes are predictors of severe clinical picture and unfavorable outcome of the disease in patients with COVID-19
- METTL3 blocked the progression of diabetic retinopathy through m6A-modified SOX2
- The predictive significance of anti-RO-52 antibody in patients with interstitial pneumonia after treatment of malignant tumors
- Exploring cerebrospinal fluid metabolites, cognitive function, and brain atrophy: Insights from Mendelian randomization
- Development and validation of potential molecular subtypes and signatures of ocular sarcoidosis based on autophagy-related gene analysis
- Widespread venous thrombosis: Unveiling a complex case of Behçet’s disease with a literature perspective
- Uterine fibroid embolization: An analysis of clinical outcomes and impact on patients’ quality of life
- Discovery of lipid metabolism-related diagnostic biomarkers and construction of diagnostic model in steroid-induced osteonecrosis of femoral head
- Serum-derived exomiR-188-3p is a promising novel biomarker for early-stage ovarian cancer
- Enhancing chronic back pain management: A comparative study of ultrasound–MRI fusion guidance for paravertebral nerve block
- Peptide CCAT1-70aa promotes hepatocellular carcinoma proliferation and invasion via the MAPK/ERK pathway
- Electroacupuncture-induced reduction of myocardial ischemia–reperfusion injury via FTO-dependent m6A methylation modulation
- Hemorrhoids and cardiovascular disease: A bidirectional Mendelian randomization study
- Cell-free adipose extract inhibits hypertrophic scar formation through collagen remodeling and antiangiogenesis
- HALP score in Demodex blepharitis: A case–control study
- Assessment of SOX2 performance as a marker for circulating cancer stem-like cells (CCSCs) identification in advanced breast cancer patients using CytoTrack system
- Risk and prognosis for brain metastasis in primary metastatic cervical cancer patients: A population-based study
- Comparison of the two intestinal anastomosis methods in pediatric patients
- Factors influencing hematological toxicity and adverse effects of perioperative hyperthermic intraperitoneal vs intraperitoneal chemotherapy in gastrointestinal cancer
- Endotoxin tolerance inhibits NLRP3 inflammasome activation in macrophages of septic mice by restoring autophagic flux through TRIM26
- Lateral transperitoneal laparoscopic adrenalectomy: A single-centre experience of 21 procedures
- Petunidin attenuates lipopolysaccharide-induced retinal microglia inflammatory response in diabetic retinopathy by targeting OGT/NF-κB/LCN2 axis
- Procalcitonin and C-reactive protein as biomarkers for diagnosing and assessing the severity of acute cholecystitis
- Factors determining the number of sessions in successful extracorporeal shock wave lithotripsy patients
- Development of a nomogram for predicting cancer-specific survival in patients with renal pelvic cancer following surgery
- Inhibition of ATG7 promotes orthodontic tooth movement by regulating the RANKL/OPG ratio under compression force
- A machine learning-based prognostic model integrating mRNA stemness index, hypoxia, and glycolysis‑related biomarkers for colorectal cancer
- Glutathione attenuates sepsis-associated encephalopathy via dual modulation of NF-κB and PKA/CREB pathways
- FAHD1 prevents neuronal ferroptosis by modulating R-loop and the cGAS–STING pathway
- Association of placenta weight and morphology with term low birth weight: A case–control study
- Investigation of the pathogenic variants induced Sjogren’s syndrome in Turkish population
- Nucleotide metabolic abnormalities in post-COVID-19 condition and type 2 diabetes mellitus patients and their association with endocrine dysfunction
- TGF-β–Smad2/3 signaling in high-altitude pulmonary hypertension in rats: Role and mechanisms via macrophage M2 polarization
- Ultrasound-guided unilateral versus bilateral erector spinae plane block for postoperative analgesia of patients undergoing laparoscopic cholecystectomy
- Profiling gut microbiome dynamics in subacute thyroiditis: Implications for pathogenesis, diagnosis, and treatment
- Delta neutrophil index, CRP/albumin ratio, procalcitonin, immature granulocytes, and HALP score in acute appendicitis: Best performing biomarker?
- Anticancer activity mechanism of novelly synthesized and characterized benzofuran ring-linked 3-nitrophenyl chalcone derivative on colon cancer cells
- H2valdien3 arrests the cell cycle and induces apoptosis of gastric cancer
- Prognostic relevance of PRSS2 and its immune correlates in papillary thyroid carcinoma
- Association of SGLT2 inhibition with psychiatric disorders: A Mendelian randomization study
- Motivational interviewing for alcohol use reduction in Thai patients
- Luteolin alleviates oxygen-glucose deprivation/reoxygenation-induced neuron injury by regulating NLRP3/IL-1β signaling
- Polyphyllin II inhibits thyroid cancer cell growth by simultaneously inhibiting glycolysis and oxidative phosphorylation
- Relationship between the expression of copper death promoting factor SLC31A1 in papillary thyroid carcinoma and clinicopathological indicators and prognosis
- CSF2 polarized neutrophils and invaded renal cancer cells in vitro influence
- Proton pump inhibitors-induced thrombocytopenia: A systematic literature analysis of case reports
- The current status and influence factors of research ability among community nurses: A sequential qualitative–quantitative study
- OKAIN: A comprehensive oncology knowledge base for the interpretation of clinically actionable alterations
- The relationship between serum CA50, CA242, and SAA levels and clinical pathological characteristics and prognosis in patients with pancreatic cancer
- Identification and external validation of a prognostic signature based on hypoxia–glycolysis-related genes for kidney renal clear cell carcinoma
- Engineered RBC-derived nanovesicles functionalized with tumor-targeting ligands: A comparative study on breast cancer targeting efficiency and biocompatibility
- Relationship of resting echocardiography combined with serum micronutrients to the severity of low-gradient severe aortic stenosis
- Effect of vibration on pain during subcutaneous heparin injection: A randomized, single-blind, placebo-controlled trial
- The diagnostic performance of machine learning-based FFRCT for coronary artery disease: A meta-analysis
- Comparing biofeedback device vs diaphragmatic breathing for bloating relief: A randomized controlled trial
- Serum uric acid to albumin ratio and C-reactive protein as predictive biomarkers for chronic total occlusion and coronary collateral circulation quality
- Multiple organ scoring systems for predicting in-hospital mortality of sepsis patients in the intensive care unit
- Single-cell RNA sequencing data analysis of the inner ear in gentamicin-treated mice via intraperitoneal injection
- Review Articles
- The effects of enhanced external counter-pulsation on post-acute sequelae of COVID-19: A narrative review
- Diabetes-related cognitive impairment: Mechanisms, symptoms, and treatments
- Microscopic changes and gross morphology of placenta in women affected by gestational diabetes mellitus in dietary treatment: A systematic review
- Review of mechanisms and frontier applications in IL-17A-induced hypertension
- Research progress on the correlation between islet amyloid peptides and type 2 diabetes mellitus
- The safety and efficacy of BCG combined with mitomycin C compared with BCG monotherapy in patients with non-muscle-invasive bladder cancer: A systematic review and meta-analysis
- The application of augmented reality in robotic general surgery: A mini-review
- The effect of Greek mountain tea extract and wheat germ extract on peripheral blood flow and eicosanoid metabolism in mammals
- Neurogasobiology of migraine: Carbon monoxide, hydrogen sulfide, and nitric oxide as emerging pathophysiological trinacrium relevant to nociception regulation
- Plant polyphenols, terpenes, and terpenoids in oral health
- Laboratory medicine between technological innovation, rights safeguarding, and patient safety: A bioethical perspective
- End-of-life in cancer patients: Medicolegal implications and ethical challenges in Europe
- The maternal factors during pregnancy for intrauterine growth retardation: An umbrella review
- Intra-abdominal hypertension/abdominal compartment syndrome of pediatric patients in critical care settings
- PI3K/Akt pathway and neuroinflammation in sepsis-associated encephalopathy
- Screening of Group B Streptococcus in pregnancy: A systematic review for the laboratory detection
- Giant borderline ovarian tumours – review of the literature
- Leveraging artificial intelligence for collaborative care planning: Innovations and impacts in shared decision-making – A systematic review
- Cholera epidemiology analysis through the experience of the 1973 Naples epidemic
- Risk factors of frailty/sarcopenia in community older adults: Meta-analysis
- Supplement strategies for infertility in overweight women: Evidence and legal insights
- Scurvy, a not obsolete disorder: Clinical report in eight young children and literature review
- A meta-analysis of the effects of DBS on cognitive function in patients with advanced PD
- Protective role of selenium in sepsis: Mechanisms and potential therapeutic strategies
- Strategies for hyperkalemia management in dialysis patients: A systematic review
- C-reactive protein-to-albumin ratio in peripheral artery disease
- Case Reports
- Delayed graft function after renal transplantation
- Semaglutide treatment for type 2 diabetes in a patient with chronic myeloid leukemia: A case report and review of the literature
- Diverse electrophysiological demyelinating features in a late-onset glycogen storage disease type IIIa case
- Giant right atrial hemangioma presenting with ascites: A case report
- Laser excision of a large granular cell tumor of the vocal cord with subglottic extension: A case report
- EsoFLIP-assisted dilation for dysphagia in systemic sclerosis: Highlighting the role of multimodal esophageal evaluation
- Molecular hydrogen-rhodiola as an adjuvant therapy for ischemic stroke in internal carotid artery occlusion: A case report
- Coronary artery anomalies: A case of the “malignant” left coronary artery and its surgical management
- Rapid Communication
- Biological properties of valve materials using RGD and EC
-
A single oral administration of flavanols enhances short
-term memory in mice along with increased brain-derived neurotrophic factor - Letter to the Editor
- Role of enhanced external counterpulsation in long COVID
- Expression of Concern
- Expression of concern “A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma”
- Expression of concern “Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway”
- Expression of concern “circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8”
- Corrigendum
- Corrigendum to “Empagliflozin improves aortic injury in obese mice by regulating fatty acid metabolism”
- Corrigendum to “Comparing the therapeutic efficacy of endoscopic minimally invasive surgery and traditional surgery for early-stage breast cancer: A meta-analysis”
- Corrigendum to “The progress of autoimmune hepatitis research and future challenges”
- Retraction
- Retraction of “miR-654-5p promotes gastric cancer progression via the GPRIN1/NF-κB pathway”
- Retraction of: “LncRNA CASC15 inhibition relieves renal fibrosis in diabetic nephropathy through downregulating SP-A by sponging to miR-424”
- Retraction of: “SCARA5 inhibits oral squamous cell carcinoma via inactivating the STAT3 and PI3K/AKT signaling pathways”
- Special Issue Advancements in oncology: bridging clinical and experimental research - Part II
- Unveiling novel biomarkers for platinum chemoresistance in ovarian cancer
- Lathyrol affects the expression of AR and PSA and inhibits the malignant behavior of RCC cells
- The era of increasing cancer survivorship: Trends in fertility preservation, medico-legal implications, and ethical challenges
- Bone scintigraphy and positron emission tomography in the early diagnosis of MRONJ
- Meta-analysis of clinical efficacy and safety of immunotherapy combined with chemotherapy in non-small cell lung cancer
- Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part IV
- Exploration of mRNA-modifying METTL3 oncogene as momentous prognostic biomarker responsible for colorectal cancer development
- Special Issue The evolving saga of RNAs from bench to bedside - Part III
- Interaction and verification of ferroptosis-related RNAs Rela and Stat3 in promoting sepsis-associated acute kidney injury
- The mRNA MOXD1: Link to oxidative stress and prognostic significance in gastric cancer
- Special Issue Exploring the biological mechanism of human diseases based on MultiOmics Technology - Part II
- Dynamic changes in lactate-related genes in microglia and their role in immune cell interactions after ischemic stroke
- A prognostic model correlated with fatty acid metabolism in Ewing’s sarcoma based on bioinformatics analysis
- Red cell distribution width predicts early kidney injury: A NHANES cross-sectional study
- Special Issue Diabetes mellitus: pathophysiology, complications & treatment
- Nutritional risk assessment and nutritional support in children with congenital diabetes during surgery
- Correlation of the differential expressions of RANK, RANKL, and OPG with obesity in the elderly population in Xinjiang
- A discussion on the application of fluorescence micro-optical sectioning tomography in the research of cognitive dysfunction in diabetes
- A review of brain research on T2DM-related cognitive dysfunction
- Metformin and estrogen modulation in LABC with T2DM: A 36-month randomized trial
- Special Issue Innovative Biomarker Discovery and Precision Medicine in Cancer Diagnostics
- CircASH1L-mediated tumor progression in triple-negative breast cancer: PI3K/AKT pathway mechanisms
Artikel in diesem Heft
- Research Articles
- Network pharmacological analysis and in vitro testing of the rutin effects on triple-negative breast cancer
- Impact of diabetes on long-term survival in elderly liver cancer patients: A retrospective study
- Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway
- Risk factors for severe adverse drug reactions in hospitalized patients
- Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing
- Development and validation of headspace gas chromatography with a flame ionization detector method for the determination of ethanol in the vitreous humor
- CMSP exerts anti-tumor effects on small cell lung cancer cells by inducing mitochondrial dysfunction and ferroptosis
- Predictive value of plasma sB7-H3 and YKL-40 in pediatric refractory Mycoplasma pneumoniae pneumonia
- Antiangiogenic potential of Elaeagnus umbellata extracts and molecular docking study by targeting VEGFR-2 pathway
- Comparison of the effectiveness of nurse-led preoperative counseling and postoperative follow-up care vs standard care for patients with gastric cancer
- Comparing the therapeutic efficacy of endoscopic minimally invasive surgery and traditional surgery for early-stage breast cancer: A meta-analysis
- Adhered macrophages as an additional marker of cardiomyocyte injury in biopsies of patients with dilated cardiomyopathy
- Association between statin administration and outcome in patients with sepsis: A retrospective study
- Exploration of the association between estimated glucose disposal rate and osteoarthritis in middle-aged and older adults: An analysis of NHANES data from 2011 to 2018
- A comparative analysis of the binary and multiclass classified chest X-ray images of pneumonia and COVID-19 with ML and DL models
- Lysophosphatidic acid 2 alleviates deep vein thrombosis via protective endothelial barrier function
- Transcription factor A, mitochondrial promotes lymph node metastasis and lymphangiogenesis in epithelial ovarian carcinoma
- Serum PM20D1 levels are associated with nutritional status and inflammatory factors in gastric cancer patients undergoing early enteral nutrition
- Hydromorphone reduced the incidence of emergence agitation after adenotonsillectomy in children with obstructive sleep apnea: A randomized, double-blind study
- Vitamin D replacement therapy may regulate sleep habits in patients with restless leg syndrome
- The first-line antihypertensive nitrendipine potentiated the therapeutic effect of oxaliplatin by downregulating CACNA1D in colorectal cancer
- Health literacy and health-related quality of life: The mediating role of irrational happiness
- Modulatory effects of Lycium barbarum polysaccharide on bone cell dynamics in osteoporosis
- Mechanism research on inhibition of gastric cancer in vitro by the extract of Pinellia ternata based on network pharmacology and cellular metabolomics
- Examination of the causal role of immune cells in non-alcoholic fatty liver disease by a bidirectional Mendelian randomization study
- Clinical analysis of ten cases of HIV infection combined with acute leukemia
- Investigating the cardioprotective potential of quercetin against tacrolimus-induced cardiotoxicity in Wistar rats: A mechanistic insights
- Clinical observation of probiotics combined with mesalazine and Yiyi Baitouweng Decoction retention enema in treating mild-to-moderate ulcerative colitis
- Diagnostic value of ratio of blood inflammation to coagulation markers in periprosthetic joint infection
- Sex-specific associations of sex hormone binding globulin and risk of bladder cancer
- Core muscle strength and stability-oriented breathing training reduces inter-recti distance in postpartum women
- The ERAS nursing care strategy for patients undergoing transsphenoidal endoscopic pituitary tumor resection: A randomized blinded controlled trial
- The serum IL-17A levels in patients with traumatic bowel rupture post-surgery and its predictive value for patient prognosis
- Impact of Kolb’s experiential learning theory-based nursing on caregiver burden and psychological state of caregivers of dementia patients
- Analysis of serum NLR combined with intraoperative margin condition to predict the prognosis of cervical HSIL patients undergoing LEEP surgery
- Commiphora gileadensis ameliorate infertility and erectile dysfunction in diabetic male mice
- The correlation between epithelial–mesenchymal transition classification and MMP2 expression of circulating tumor cells and prognosis of advanced or metastatic nasopharyngeal carcinoma
- Tetrahydropalmatine improves mitochondrial function in vascular smooth muscle cells of atherosclerosis in vitro by inhibiting Ras homolog gene family A/Rho-associated protein kinase-1 signaling pathway
- A cross-sectional study: Relationship between serum oxidative stress levels and arteriovenous fistula maturation in maintenance dialysis patients
- A comparative analysis of the impact of repeated administration of flavan 3-ol on brown, subcutaneous, and visceral adipose tissue
- Identifying early screening factors for depression in middle-aged and older adults: A cohort study
- Perform tumor-specific survival analysis for Merkel cell carcinoma patients undergoing surgical resection based on the SEER database by constructing a nomogram chart
- Unveiling the role of CXCL10 in pancreatic cancer progression: A novel prognostic indicator
- High-dose preoperative intraperitoneal erythropoietin and intravenous methylprednisolone in acute traumatic spinal cord injuries following decompression surgeries
- RAB39B: A novel biomarker for acute myeloid leukemia identified via multi-omics and functional validation
- Impact of peripheral conditioning on reperfusion injury following primary percutaneous coronary intervention in diabetic and non-diabetic STEMI patients
- Clinical efficacy of azacitidine in the treatment of middle- and high-risk myelodysplastic syndrome in middle-aged and elderly patients: A retrospective study
- The effect of ambulatory blood pressure load on mitral regurgitation in continuous ambulatory peritoneal dialysis patients
- Expression and clinical significance of ITGA3 in breast cancer
- Single-nucleus RNA sequencing reveals ARHGAP28 expression of podocytes as a biomarker in human diabetic nephropathy
- rSIG combined with NLR in the prognostic assessment of patients with multiple injuries
- Toxic metals and metalloids in collagen supplements of fish and jellyfish origin: Risk assessment for daily intake
- Exploring causal relationship between 41 inflammatory cytokines and marginal zone lymphoma: A bidirectional Mendelian randomization study
- Gender beliefs and legitimization of dating violence in adolescents
- Effect of serum IL-6, CRP, and MMP-9 levels on the efficacy of modified preperitoneal Kugel repair in patients with inguinal hernia
- Effect of smoking and smoking cessation on hematological parameters in polycythemic patients
- Pathogen surveillance and risk factors for pulmonary infection in patients with lung cancer: A retrospective single-center study
- Necroptosis of hippocampal neurons in paclitaxel chemotherapy-induced cognitive impairment mediates microglial activation via TLR4/MyD88 signaling pathway
- Celastrol suppresses neovascularization in rat aortic vascular endothelial cells stimulated by inflammatory tenocytes via modulating the NLRP3 pathway
- Cord-lamina angle and foraminal diameter as key predictors of C5 palsy after anterior cervical decompression and fusion surgery
- GATA1: A key biomarker for predicting the prognosis of patients with diffuse large B-cell lymphoma
- Influencing factors of false lumen thrombosis in type B aortic dissection: A single-center retrospective study
- MZB1 regulates the immune microenvironment and inhibits ovarian cancer cell migration
- Integrating experimental and network pharmacology to explore the pharmacological mechanisms of Dioscin against glioblastoma
- Trends in research on preterm birth in twin pregnancy based on bibliometrics
- Four-week IgE/baseline IgE ratio combined with tryptase predicts clinical outcome in omalizumab-treated children with moderate-to-severe asthma
- Single-cell transcriptomic analysis identifies a stress response Schwann cell subtype
- Acute pancreatitis risk in the diagnosis and management of inflammatory bowel disease: A critical focus
- Effect of subclinical esketamine on NLRP3 and cognitive dysfunction in elderly ischemic stroke patients
- Interleukin-37 mediates the anti-oral tumor activity in oral cancer through STAT3
- CA199 and CEA expression levels, and minimally invasive postoperative prognosis analysis in esophageal squamous carcinoma patients
- Efficacy of a novel drainage catheter in the treatment of CSF leak after posterior spine surgery: A retrospective cohort study
- Comprehensive biomedicine assessment of Apteranthes tuberculata extracts: Phytochemical analysis and multifaceted pharmacological evaluation in animal models
- Relation of time in range to severity of coronary artery disease in patients with type 2 diabetes: A cross-sectional study
- Dopamine attenuates ethanol-induced neuronal apoptosis by stimulating electrical activity in the developing rat retina
- Correlation between albumin levels during the third trimester and the risk of postpartum levator ani muscle rupture
- Factors associated with maternal attention and distraction during breastfeeding and childcare: A cross-sectional study in the west of Iran
- Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments
- The law on oncological oblivion in the Italian and European context: How to best uphold the cancer patients’ rights to privacy and self-determination?
- The prognostic value of the neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and prognostic nutritional index for survival in patients with colorectal cancer
- Factors affecting the measurements of peripheral oxygen saturation values in healthy young adults
- Comparison and correlations between findings of hysteroscopy and vaginal color Doppler ultrasonography for detection of uterine abnormalities in patients with recurrent implantation failure
- The effects of different types of RAGT on balance function in stroke patients with low levels of independent walking in a convalescent rehabilitation hospital
- Causal relationship between asthma and ankylosing spondylitis: A bidirectional two-sample univariable and multivariable Mendelian randomization study
- Correlations of health literacy with individuals’ understanding and use of medications in Southern Taiwan
- Correlation of serum calprotectin with outcome of acute cerebral infarction
- Comparison of computed tomography and guided bronchoscopy in the diagnosis of pulmonary nodules: A systematic review and meta-analysis
- Curdione protects vascular endothelial cells and atherosclerosis via the regulation of DNMT1-mediated ERBB4 promoter methylation
- The identification of novel missense variant in ChAT gene in a patient with gestational diabetes denotes plausible genetic association
- Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance
- Exploring the role of succinyl carnitine in the association between CD39⁺ CD4⁺ T cell and ulcerative colitis: A Mendelian randomization study
- Dexmedetomidine suppresses microglial activation in postoperative cognitive dysfunction via the mmu-miRNA-125/TRAF6 signaling axis
- Analysis of serum metabolomics in patients with different types of chronic heart failure
- Diagnostic value of hematological parameters in the early diagnosis of acute cholecystitis
- Pachymaran alleviates fat accumulation, hepatocyte degeneration, and injury in mice with nonalcoholic fatty liver disease
- Decrease in CD4 and CD8 lymphocytes are predictors of severe clinical picture and unfavorable outcome of the disease in patients with COVID-19
- METTL3 blocked the progression of diabetic retinopathy through m6A-modified SOX2
- The predictive significance of anti-RO-52 antibody in patients with interstitial pneumonia after treatment of malignant tumors
- Exploring cerebrospinal fluid metabolites, cognitive function, and brain atrophy: Insights from Mendelian randomization
- Development and validation of potential molecular subtypes and signatures of ocular sarcoidosis based on autophagy-related gene analysis
- Widespread venous thrombosis: Unveiling a complex case of Behçet’s disease with a literature perspective
- Uterine fibroid embolization: An analysis of clinical outcomes and impact on patients’ quality of life
- Discovery of lipid metabolism-related diagnostic biomarkers and construction of diagnostic model in steroid-induced osteonecrosis of femoral head
- Serum-derived exomiR-188-3p is a promising novel biomarker for early-stage ovarian cancer
- Enhancing chronic back pain management: A comparative study of ultrasound–MRI fusion guidance for paravertebral nerve block
- Peptide CCAT1-70aa promotes hepatocellular carcinoma proliferation and invasion via the MAPK/ERK pathway
- Electroacupuncture-induced reduction of myocardial ischemia–reperfusion injury via FTO-dependent m6A methylation modulation
- Hemorrhoids and cardiovascular disease: A bidirectional Mendelian randomization study
- Cell-free adipose extract inhibits hypertrophic scar formation through collagen remodeling and antiangiogenesis
- HALP score in Demodex blepharitis: A case–control study
- Assessment of SOX2 performance as a marker for circulating cancer stem-like cells (CCSCs) identification in advanced breast cancer patients using CytoTrack system
- Risk and prognosis for brain metastasis in primary metastatic cervical cancer patients: A population-based study
- Comparison of the two intestinal anastomosis methods in pediatric patients
- Factors influencing hematological toxicity and adverse effects of perioperative hyperthermic intraperitoneal vs intraperitoneal chemotherapy in gastrointestinal cancer
- Endotoxin tolerance inhibits NLRP3 inflammasome activation in macrophages of septic mice by restoring autophagic flux through TRIM26
- Lateral transperitoneal laparoscopic adrenalectomy: A single-centre experience of 21 procedures
- Petunidin attenuates lipopolysaccharide-induced retinal microglia inflammatory response in diabetic retinopathy by targeting OGT/NF-κB/LCN2 axis
- Procalcitonin and C-reactive protein as biomarkers for diagnosing and assessing the severity of acute cholecystitis
- Factors determining the number of sessions in successful extracorporeal shock wave lithotripsy patients
- Development of a nomogram for predicting cancer-specific survival in patients with renal pelvic cancer following surgery
- Inhibition of ATG7 promotes orthodontic tooth movement by regulating the RANKL/OPG ratio under compression force
- A machine learning-based prognostic model integrating mRNA stemness index, hypoxia, and glycolysis‑related biomarkers for colorectal cancer
- Glutathione attenuates sepsis-associated encephalopathy via dual modulation of NF-κB and PKA/CREB pathways
- FAHD1 prevents neuronal ferroptosis by modulating R-loop and the cGAS–STING pathway
- Association of placenta weight and morphology with term low birth weight: A case–control study
- Investigation of the pathogenic variants induced Sjogren’s syndrome in Turkish population
- Nucleotide metabolic abnormalities in post-COVID-19 condition and type 2 diabetes mellitus patients and their association with endocrine dysfunction
- TGF-β–Smad2/3 signaling in high-altitude pulmonary hypertension in rats: Role and mechanisms via macrophage M2 polarization
- Ultrasound-guided unilateral versus bilateral erector spinae plane block for postoperative analgesia of patients undergoing laparoscopic cholecystectomy
- Profiling gut microbiome dynamics in subacute thyroiditis: Implications for pathogenesis, diagnosis, and treatment
- Delta neutrophil index, CRP/albumin ratio, procalcitonin, immature granulocytes, and HALP score in acute appendicitis: Best performing biomarker?
- Anticancer activity mechanism of novelly synthesized and characterized benzofuran ring-linked 3-nitrophenyl chalcone derivative on colon cancer cells
- H2valdien3 arrests the cell cycle and induces apoptosis of gastric cancer
- Prognostic relevance of PRSS2 and its immune correlates in papillary thyroid carcinoma
- Association of SGLT2 inhibition with psychiatric disorders: A Mendelian randomization study
- Motivational interviewing for alcohol use reduction in Thai patients
- Luteolin alleviates oxygen-glucose deprivation/reoxygenation-induced neuron injury by regulating NLRP3/IL-1β signaling
- Polyphyllin II inhibits thyroid cancer cell growth by simultaneously inhibiting glycolysis and oxidative phosphorylation
- Relationship between the expression of copper death promoting factor SLC31A1 in papillary thyroid carcinoma and clinicopathological indicators and prognosis
- CSF2 polarized neutrophils and invaded renal cancer cells in vitro influence
- Proton pump inhibitors-induced thrombocytopenia: A systematic literature analysis of case reports
- The current status and influence factors of research ability among community nurses: A sequential qualitative–quantitative study
- OKAIN: A comprehensive oncology knowledge base for the interpretation of clinically actionable alterations
- The relationship between serum CA50, CA242, and SAA levels and clinical pathological characteristics and prognosis in patients with pancreatic cancer
- Identification and external validation of a prognostic signature based on hypoxia–glycolysis-related genes for kidney renal clear cell carcinoma
- Engineered RBC-derived nanovesicles functionalized with tumor-targeting ligands: A comparative study on breast cancer targeting efficiency and biocompatibility
- Relationship of resting echocardiography combined with serum micronutrients to the severity of low-gradient severe aortic stenosis
- Effect of vibration on pain during subcutaneous heparin injection: A randomized, single-blind, placebo-controlled trial
- The diagnostic performance of machine learning-based FFRCT for coronary artery disease: A meta-analysis
- Comparing biofeedback device vs diaphragmatic breathing for bloating relief: A randomized controlled trial
- Serum uric acid to albumin ratio and C-reactive protein as predictive biomarkers for chronic total occlusion and coronary collateral circulation quality
- Multiple organ scoring systems for predicting in-hospital mortality of sepsis patients in the intensive care unit
- Single-cell RNA sequencing data analysis of the inner ear in gentamicin-treated mice via intraperitoneal injection
- Review Articles
- The effects of enhanced external counter-pulsation on post-acute sequelae of COVID-19: A narrative review
- Diabetes-related cognitive impairment: Mechanisms, symptoms, and treatments
- Microscopic changes and gross morphology of placenta in women affected by gestational diabetes mellitus in dietary treatment: A systematic review
- Review of mechanisms and frontier applications in IL-17A-induced hypertension
- Research progress on the correlation between islet amyloid peptides and type 2 diabetes mellitus
- The safety and efficacy of BCG combined with mitomycin C compared with BCG monotherapy in patients with non-muscle-invasive bladder cancer: A systematic review and meta-analysis
- The application of augmented reality in robotic general surgery: A mini-review
- The effect of Greek mountain tea extract and wheat germ extract on peripheral blood flow and eicosanoid metabolism in mammals
- Neurogasobiology of migraine: Carbon monoxide, hydrogen sulfide, and nitric oxide as emerging pathophysiological trinacrium relevant to nociception regulation
- Plant polyphenols, terpenes, and terpenoids in oral health
- Laboratory medicine between technological innovation, rights safeguarding, and patient safety: A bioethical perspective
- End-of-life in cancer patients: Medicolegal implications and ethical challenges in Europe
- The maternal factors during pregnancy for intrauterine growth retardation: An umbrella review
- Intra-abdominal hypertension/abdominal compartment syndrome of pediatric patients in critical care settings
- PI3K/Akt pathway and neuroinflammation in sepsis-associated encephalopathy
- Screening of Group B Streptococcus in pregnancy: A systematic review for the laboratory detection
- Giant borderline ovarian tumours – review of the literature
- Leveraging artificial intelligence for collaborative care planning: Innovations and impacts in shared decision-making – A systematic review
- Cholera epidemiology analysis through the experience of the 1973 Naples epidemic
- Risk factors of frailty/sarcopenia in community older adults: Meta-analysis
- Supplement strategies for infertility in overweight women: Evidence and legal insights
- Scurvy, a not obsolete disorder: Clinical report in eight young children and literature review
- A meta-analysis of the effects of DBS on cognitive function in patients with advanced PD
- Protective role of selenium in sepsis: Mechanisms and potential therapeutic strategies
- Strategies for hyperkalemia management in dialysis patients: A systematic review
- C-reactive protein-to-albumin ratio in peripheral artery disease
- Case Reports
- Delayed graft function after renal transplantation
- Semaglutide treatment for type 2 diabetes in a patient with chronic myeloid leukemia: A case report and review of the literature
- Diverse electrophysiological demyelinating features in a late-onset glycogen storage disease type IIIa case
- Giant right atrial hemangioma presenting with ascites: A case report
- Laser excision of a large granular cell tumor of the vocal cord with subglottic extension: A case report
- EsoFLIP-assisted dilation for dysphagia in systemic sclerosis: Highlighting the role of multimodal esophageal evaluation
- Molecular hydrogen-rhodiola as an adjuvant therapy for ischemic stroke in internal carotid artery occlusion: A case report
- Coronary artery anomalies: A case of the “malignant” left coronary artery and its surgical management
- Rapid Communication
- Biological properties of valve materials using RGD and EC
-
A single oral administration of flavanols enhances short
-term memory in mice along with increased brain-derived neurotrophic factor - Letter to the Editor
- Role of enhanced external counterpulsation in long COVID
- Expression of Concern
- Expression of concern “A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma”
- Expression of concern “Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway”
- Expression of concern “circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8”
- Corrigendum
- Corrigendum to “Empagliflozin improves aortic injury in obese mice by regulating fatty acid metabolism”
- Corrigendum to “Comparing the therapeutic efficacy of endoscopic minimally invasive surgery and traditional surgery for early-stage breast cancer: A meta-analysis”
- Corrigendum to “The progress of autoimmune hepatitis research and future challenges”
- Retraction
- Retraction of “miR-654-5p promotes gastric cancer progression via the GPRIN1/NF-κB pathway”
- Retraction of: “LncRNA CASC15 inhibition relieves renal fibrosis in diabetic nephropathy through downregulating SP-A by sponging to miR-424”
- Retraction of: “SCARA5 inhibits oral squamous cell carcinoma via inactivating the STAT3 and PI3K/AKT signaling pathways”
- Special Issue Advancements in oncology: bridging clinical and experimental research - Part II
- Unveiling novel biomarkers for platinum chemoresistance in ovarian cancer
- Lathyrol affects the expression of AR and PSA and inhibits the malignant behavior of RCC cells
- The era of increasing cancer survivorship: Trends in fertility preservation, medico-legal implications, and ethical challenges
- Bone scintigraphy and positron emission tomography in the early diagnosis of MRONJ
- Meta-analysis of clinical efficacy and safety of immunotherapy combined with chemotherapy in non-small cell lung cancer
- Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part IV
- Exploration of mRNA-modifying METTL3 oncogene as momentous prognostic biomarker responsible for colorectal cancer development
- Special Issue The evolving saga of RNAs from bench to bedside - Part III
- Interaction and verification of ferroptosis-related RNAs Rela and Stat3 in promoting sepsis-associated acute kidney injury
- The mRNA MOXD1: Link to oxidative stress and prognostic significance in gastric cancer
- Special Issue Exploring the biological mechanism of human diseases based on MultiOmics Technology - Part II
- Dynamic changes in lactate-related genes in microglia and their role in immune cell interactions after ischemic stroke
- A prognostic model correlated with fatty acid metabolism in Ewing’s sarcoma based on bioinformatics analysis
- Red cell distribution width predicts early kidney injury: A NHANES cross-sectional study
- Special Issue Diabetes mellitus: pathophysiology, complications & treatment
- Nutritional risk assessment and nutritional support in children with congenital diabetes during surgery
- Correlation of the differential expressions of RANK, RANKL, and OPG with obesity in the elderly population in Xinjiang
- A discussion on the application of fluorescence micro-optical sectioning tomography in the research of cognitive dysfunction in diabetes
- A review of brain research on T2DM-related cognitive dysfunction
- Metformin and estrogen modulation in LABC with T2DM: A 36-month randomized trial
- Special Issue Innovative Biomarker Discovery and Precision Medicine in Cancer Diagnostics
- CircASH1L-mediated tumor progression in triple-negative breast cancer: PI3K/AKT pathway mechanisms
