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Impact of “3414” fertilization on the yield and quality of greenhouse tomatoes

  • Chunyan Wu , Xiaoyi Han , Yan Cheng , Xueke Wang and Wei Wang EMAIL logo
Published/Copyright: June 28, 2024
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Open Life Sciences
From the journal Open Life Sciences Volume 19 Issue 1

Abstract

This study aimed to explore the effects of different nitrogen, phosphorus, and potassium ratios on the yield and nutritional quality of greenhouse tomatoes under a water and fertilizer integration model. Greenhouse tomatoes were used as the research object, and the “3414” fertilizer trial design was employed to assess tomato growth, yield, quality, and soil indicators across various treatment combinations. The goal was to determine the optimal fertilization scheme and recommend appropriate fertilizer quantities for tomato cultivation and production. The results revealed that different fertilizer ratios significantly affected both the quality and yield of tomatoes. Overall, the tomato yield tended to increase with higher fertilization amounts, with potassium exhibiting the most pronounced effect on yield increase, followed by phosphorus and nitrogen. The comprehensive analysis of principal components indicated that the N2P2K1 treatment yielded the highest nutritional quality and yield. Therefore, the best fertilization combination identified in this study consisted of nitrogen fertilizer at 197.28 kg hm−2, phosphorus fertilizer at 88.75 kg hm−2, and potassium fertilizer at 229.80 kg hm−2. These findings provided the scientific basis for optimizing fertilization practices in greenhouse tomato cultivation and production in the Jilin Province.

Keywords: “3414” experimental design; quality; tomato; yield

1 Introduction

Tomato (Lycopersicon esculentum) is an annual or perennial herbaceous plant belonging to the Solanaceae family. It is extensively cultivated in both northern and southern regions of China. It ranks among the most commonly grown greenhouse vegetables globally [1]. Tomatoes are characterized by their vibrant colors, distinctive flavor, and high contents of nutrients, such as carotene, lycopene, and vitamins C and B. Besides their nutritional value, tomatoes possess high medicinal value, aiding digestion, detoxification, and preventing cardiovascular diseases [2,3]. Given their high water and nutrient requirements, greenhouse tomato cultivation has gained widespread adoption, ensuring a steady supply of fresh produce. Integrated water and fertilizer technologies represent advanced fertilizer management techniques that enhance water and nutrient utilization efficiency, ultimately maximizing economic returns [4,5]. Fertilization is a pivotal technique in tomato cultivation and production, playing a crucial role in improving soil fertility, facilitating tomato growth and development, and increasing yield and quality [6,7]. However, the indiscriminate use of fertilizers can have negative consequences [8,9]. Therefore, appropriate fertilization practices are essential for promoting agricultural productivity and achieving superior crop quality and yield.

Nitrogen, phosphorus, and potassium are essential elements for the normal growth and development of plants. Improper combinations of these three elements can lead to crop nutrient imbalances, increased fruit nitrate content, decreased quality, low yield, and imbalanced soil nutrient supply [10,11,12]. Studies on the impact of nitrogen, phosphorus, and potassium fertilizers on tomatoes exist [13,14]. However, previous studies have examined only a limited range of nitrogen, phosphorus, and potassium ratios, neglecting the interactive effects and influence of multiple levels. In addition, the variations in soil fertility, crop residues, and cultivated varieties can alter the optimal fertilization scheme for crops. The “3414” experimental design plays a significant role in national soil testing and formula fertilization efforts. It can determine the appropriate application amounts of nitrogen, phosphorus, and potassium based on soil fertility, crop fertilization requirements, and fertilizer response functions. This experimental plan is extensively used for studying fertilizer efficiency both domestically and internationally [15]. The high-quality and efficient cultivation of greenhouse tomatoes in the Jilin Province holds significant economic importance for greenhouse film industries. This study was based on the integrated water and fertilizer technology for tomatoes and aimed to strengthen the research on rational fertilization techniques for greenhouse tomatoes. Employing the “3414” experimental design, we investigated the combined effects of nitrogen, phosphorus, and potassium application on the growth and quality of tomatoes in greenhouse facilities. The goal was to determine the optimal fertilization ratio to improve the tomato yield and quality, providing a theoretical basis for scientifically sound fertilization practices in high-quality tomato production and cultivation.

2 Materials and methods

2.1 Study location and materials

The study was conducted at the teaching and research base of Jilin Agricultural University, situated at 125°41′ East longitude and 43°82′ North latitude. The area experiences a temperate continental semi-humid monsoon climate characterized by an average annual sunshine duration of 2544.8 h, an effective accumulated temperature of 2,850℃, a frost-free period of 145 days, an average annual temperature of 5.8℃, and an average annual precipitation of 558 mm. The soil used in the study was black soil, possessing the following basic physical and chemical properties: bulk density of 0.97 g cm−3, total porosity of 59.66%, soil pH of 6.7, organic matter content of 34.39 g kg−1, alkali nitrogen content of 161 mg kg−1, available phosphorus content of 50.91 mg kg−1, and available potassium content of 190.04 mg kg−1. The tomato variety selected for the experiment was “Shidun 197,” characterized by an indeterminate growth type with medium early maturity. The fertilizers used included urea (N: 46%), calcium superphosphate (P2O5: 12%), and potassium sulfate (K2O: 50%).

2.2 Study design

The tomato seeds were sown in March 2023, using pot planting experiments, and later transplanted on May 6, with 5 plants per treatment and 3 repetitions, totaling 210 plants. During the growth period, single-stem pruning was conducted, with five fruit clusters retained per plant. The harvesting started on July 15, 2023. The study employed the “3414” fertilizer trial plan, with nitrogen, phosphorus, and potassium serving as the 3 elements, each at 0, 1, 2, and 3 levels, resulting in 14 treatments. The experimental factors and levels are detailed in Table 1, and the experimental treatments and corresponding fertilization amounts are provided in Table 2. Fertilization started 10 days after planting, facilitated by a fertigation system for integrated irrigation and fertilization management. The fertilizer application adhered to the critical growth periods of tomatoes, with 15% of the total supply administered during the planting-to-flowering phase and 85% during the fruiting period. Additionally, the water supply was adjusted following tomato growth and prevailing weather conditions.

Table 1

Nitrogen, phosphorus, and potassium fertilization levels

Fertilizer level code N (kg hm−2) P2O5 (kg hm−2) K2O (kg hm−2)
0 0.00 0.00 0.00
1 98.64 44.38 229.80
2 197.28 88.75 459.60
3 295.92 133.13 689.40
Table 2

Experimental treatments and fertilizer application amount

Treatment Fertilizer application amount (kg hm−2)
N P2O5 K2O
N0P0K0 0.00 0.00 0.00
N0P2K2 0.00 88.75 459.60
N1P2K2 98.64 88.75 459.60
N2P0K2 197.28 0.00 459.60
N2P1K2 197.28 44.38 459.60
N2P2K2 197.28 88.75 459.60
N2P3K2 197.28 133.13 459.60
N2P2K0 197.28 88.75 0.00
N2P2K1 197.28 88.75 229.80
N2P2K3 197.28 88.75 689.40
N3P2K2 295.92 88.75 459.60
N1P1K2 98.64 44.38 459.60
N1P2K1 98.64 88.75 229.80
N2P1K1 197.28 44.38 229.80

Note: The N0P0K0 treatment in the table serves as the control treatment (CK).

2.3 Measurement indicators and methods

The soil pH value was measured using the potentiometric method with a pHS-3E acidity meter (Shanghai Yoke Instrument Co., Ltd.). The electrical conductivity (EC) value was determined using the conductivity method with a DDS-11A electromagnetic conductivity meter (Shanghai Yoke Instrument Co., Ltd.). The alkali nitrogen content was determined using the NaOH alkali diffusion–absorption method. The available phosphorus content was determined using the NaHCO3 extraction–molybdenum antimony colorimetric method. The available potassium content was determined using the NH4OAC extraction-flame photometry method [16].

The plant height was measured using a tape measure from the first leaf to the top growth point. The stem thickness was measured using a Vernier caliper at the midpoint between the first and second leaves. The tomato leaf area was obtained using the weighing method. Starting from the tomato harvest period, the yield for each experimental treatment was determined, and the yield per plant was recorded.

The soluble sugar content of tomato fruit was measured using the anthrone method. The soluble protein content was determined using the Coomassie brilliant blue G-250 staining method. The soluble solid contents were measured using a refractometer. The organic acid contents were determined using acid–base titration. The vitamin C content was determined using the molybdenum blue colorimetric method. The nitrate and lycopene contents were determined using ultraviolet spectrophotometry [17,18].

2.4 Statistical analysis

The experimental data were organized using Microsoft Excel 2021. Significance testing and principal component analysis were conducted using SPSS version 26 statistical analysis software.

3 Results

3.1 Effects of different nitrogen, phosphorus, and potassium ratios on the physical and chemical properties of soil for tomato cultivation

The physical and chemical properties of the experimental soil under different fertilization treatments are presented in Table 3. The soil pH under the CK treatment was the highest (7.39), significantly surpassing that under other treatments. Conversely, the pH of the N3P2K2 treatment was the lowest (6.32), indicating that the high-nitrogen fertilizer treatment resulted in decreased pH levels. This decline in soil pH could be attributed to long-term excessive fertilization. The EC value was the highest under the N3P2K2 treatment (1145.33 µs cm−1), significantly exceeding that under other treatments. Conversely, the N0P2K2 treatment exhibited the lowest EC value (317.23 µs cm−1), indicating that the application of higher amounts of fertilizer led to increased soil EC values. This elevation implied a higher salt content in the soil, potentially impeding plant growth and development. The highest alkali nitrogen content was recorded under the N3P2K2 treatment (172.67 mg kg−1), followed by that under the N2P2K3 and N2P2K2 treatments (169.17 and 166.83 mg kg−1), respectively.

Table 3

Effects of different nitrogen, phosphorus, and potassium ratios on the physicochemical properties of soil for tomato cultivation

Treatment pH EC (µs cm−1) Alkaline nitrogen (mg kg−1) Available phosphorus (mg kg−1) Available potassium (mg kg−1)
N0P0K0 7.39 ± 0.01a 436.00 ± 3.06f 114.33 ± 1.17g 35.82 ± 0.25f 160.11 ± 0.86fg
N0P2K2 7.20 ± 0.01b 317.23 ± 0.84h 121.33 ± 3.09f 40.36 ± 0.67def 208.85 ± 1.71c
N1P2K2 7.01 ± 0.01d 579.33 ± 41.70d 128.33 ± 2.33ef 44.34 ± 0.67cd 211.42 ± 0.86c
N2P0K2 6.93 ± 0.01e 342.80 ± 1.65h 135.33 ± 1.17de 38.54 ± 1.75ef 219.11 ± 2.26b
N2P1K2 7.10 ± 0.01c 732.00 ± 4.16c 151.67 ± 1.17b 39.80 ± 1.56def 195.17 ± 1.48d
N2P2K2 7.00 ± 0.01d 458.33 ± 8.88f 166.83 ± 4.67a 46.51 ± 0.71bc 183.20 ± 1.71e
N2P3K2 6.72 ± 0.03f 813.33 ± 6.74b 156.33 ± 2.33b 54.13 ± 1.68a 182.35 ± 2.96e
N2P2K0 7.12 ± 0.02c 451.67 ± 8.65f 138.83 ± 1.17cd 41.06 ± 1.65de 158.40 ± 0.86g
N2P2K1 6.66 ± 0.02f 378.17 ± 7.19g 138.83 ± 3.07cd 44.48 ± 0.92 cd 164.39 ± 2.57fg
N2P2K3 6.65 ± 0.01f 589.67 ± 2.03d 169.17 ± 1.17a 52.31 ± 1.78a 230.23 ± 0.86a
N3P2K2 6.32 ± 0.03g 1145.33 ± 3.93a 172.67 ± 1.17a 50.00 ± 2.58ab 190.90 ± 1.71d
N1P1K2 7.10 ± 0.01c 523.67 ± 2.19e 128.33 ± 1.17ef 49.38 ± 1.21ab 222.53 ± 3.08b
N1P2K1 6.92 ± 0.03e 452.33 ± 2.33f 128.33 ± 3.07ef 51.05 ± 2.47ab 165.24 ± 3.08f
N2P1K1 6.88 ± 0.06e 398.03 ± 4.01g 143.50 ± 2.02c 39.10 ± 0.53ef 195.17 ± 2.57d

Note: The data in the table represent mean ± standard deviation. Different letters following the numbers in the same column indicate significant differences at the 0.05 level.

The lowest alkali nitrogen content was recorded under the CK treatment (114.33 mg kg−1), indicating that applying nitrogen fertilizers increases the alkali nitrogen content in the soil. The available phosphorus content was the highest under the N2P2K3 treatment (54.13 mg kg−1), followed by that under the N2P2K3 treatment (52.31 mg kg−1), with no significant difference compared with that under N1P2K1, N3P2K2, and N1P1K2 treatments but with significant difference compared with that under other treatments. Conversely, the available phosphorus content was the highest under the CK treatment (35.82 mg kg−1), indicating that soil without phosphorus fertilizers had a lower available phosphorus content. Moreover, the available potassium content was the highest under the N2P2K3 treatment (230.23 mg kg−1), which was significantly higher than that under other treatments. Conversely, the available potassium content was the lowest under the N2P2K0 treatment (158.40 mg kg−1), indicating that applying potassium fertilizers significantly increases the available potassium content in the soil.

3.2 Effects of different nitrogen, phosphorus, and potassium ratios on the growth indicators of tomato plants

As depicted in Table 4, the tallest plant was observed under the N0P2K2 treatment (133.23 cm), followed by 132.10 cm under the CK treatment. Conversely, the plant heights under N2P2K2, N1P2K1, N1P2K2, and N1P1K2 treatments were significantly lower than those under N0P2K2 and CK treatments. The plants were the shortest under the N1P1K2 treatment (94.30 cm). The CK treatment, with no fertilizer, led to better plant growth, indicating that the soil fertilizers used during the experiment ensured the normal growth of tomatoes in the early stage. Additionally, using equivalent nitrogen fertilizer amounts, the combined application of phosphorus and potassium fertilizers positively impacted the plant growth.

Table 4

Effects of different nitrogen, phosphorus, and potassium ratios on tomato growth indexes

Treatment Plant height (cm) Stem diameter (mm) Leaf area (cm2) Dry matter accumulation (g/plant)
N0P0K0 132.10 ± 0.25a 11.51 ± 0.28abc 72.07 ± 1.65abcde 194.47 ± 2.02d
N0P2K2 133.23 ± 5.75a 11.96 ± 0.94ab 74.44 ± 1.40abcd 240.10 ± 0.63c
N1P2K2 95.13 ± 10.49b 10.06 ± 1.01bcd 63.53 ± 1.80e 244.83 ± 7.68c
N2P0K2 117.60 ± 3.40ab 11.15 ± 0.64abcd 79.73 ± 2.27a 237.51 ± 12.13c
N2P1K2 118.93 ± 12.79ab 11.38 ± 0.38abc 65.51 ± 4.84de 247.90 ± 7.32c
N2P2K2 95.97 ± 6.87b 9.34 ± 0.31d 70.22 ± 2.49abcde 281.12 ± 2.37b
N2P3K2 109.57 ± 6.18ab 9.24 ± 0.20d 71.24 ± 2.33abcde 198.83 ± 5.26d
N2P2K0 114.57 ± 11.31ab 10.76 ± 0.43abcd 64.92 ± 1.42de 208.10 ± 2.17d
N2P2K1 106.13 ± 12.37ab 11.79 ± 0.87ab 70.99 ± 6.48abcde 298.89 ± 3.12a
N2P2K3 101.87 ± 10.69ab 10.15 ± 0.23bcd 77.04 ± 3.06ab 232.50 ± 3.07c
N3P2K2 121.07 ± 9.76ab 11.30 ± 0.65abc 67.52 ± 1.81bcde 199.03 ± 6.10d
N1P1K2 94.30 ± 8.99b 10.87 ± 0.35abcd 67.16 ± 2.32cde 206.41 ± 5.88d
N1P2K1 95.60 ± 4.57b 9.63 ± 0.74 cd 66.12 ± 1.48de 195.62 ± 3.30d
N2P1K1 112.40 ± 15.20ab 12.13 ± 0.10a 76.59 ± 1.63abc 310.29 ± 5.94a

Note: The data in the table represent mean ± standard deviation. Different letters following the numbers in the same column indicate significant differences at the 0.05 level.

The stem thickness was the highest under the N2P1K1 treatment (12.13 mm), significantly differing from that under N2P2K3, N1P2K2, N1P2K1, N2P2K2, and N2P3K2 treatments. This represented a 5.39% increase compared with the CK treatment. Conversely, the stem thickness was the lowest under the N2P3K2 treatment (9.24 mm), significantly differing from that under N3P2K2, N2P1K2, CK, N2P2K1, N0P2K2, and N2P1K1 treatments. These findings suggested that the application of appropriate phosphorus and potassium amounts could enhance tomato stem thickening within a certain range.

The leaf area was the largest under the N2P1K1 treatment (79.72 cm²), followed by 77.04 and 76.59 cm² under the N2P2K3 and N2P1K1 treatments, respectively. These represented increases of 10.61, 6.70, and 6.27%, respectively, compared with the CK treatment. Conversely, the leaf area was the smallest under the N1P2K2 treatment (63.53 cm). However, the leaf area under the N2P2K3 and N2P2K0 treatments did not significantly differ, with the former showing an 18.67% increase, indicating that potassium fertilizer application at the same nitrogen and phosphorus levels was beneficial for increasing tomato leaf area.

Dry matter accumulation in tomato plants was the highest under the N2P1K1 treatment (310.29 g/plant), followed by 298.89 g/plant under the N2P2K1 treatment. Both values significantly exceeded those observed under other treatments, with increases of 59.56 and 53.68%, respectively, compared with that under the CK treatment, which had the lowest accumulation at 194.47 g/plant. Additionally, dry matter accumulation in tomatoes exhibited a trend of initially increasing and then decreasing with an increase in the amounts of nitrogen, phosphorus, and potassium fertilizers.

3.3 Effects of different nitrogen, phosphorus, and potassium ratios on the tomato yield

As depicted in Table 5, the average weight of a single tomato fruit was the highest under the N2P1K1 treatment (167.42 g), followed by 161.23 g under the N2P2K1 treatment. It was significantly higher than that under the CK, N2P2K0, and N1P2K1 treatments, representing increases by 26.15 and 21.49%, respectively, compared with that under the CK treatment.

Table 5

Effects of different nitrogen, phosphorus, and potassium ratios on the tomato yield

Treatment Weight of a single fruit (g) Yield per plant (kg/plant) Plot yield (kg) Total yield (kg hm−2)
N0P0K0 132.71 ± 4.39e 2.03 ± 0.03c 30.48 ± 0.42c 76204.00 ± 1050.74c
N0P2K2 150.27 ± 3.15bcd 2.45 ± 0.03abc 36.79 ± 0.52abc 91980.75 ± 1294.39abc
N1P2K2 149.01 ± 2.32bcd 2.63 ± 0.16ab 39.40 ± 2.43ab 98491.56 ± 6084.07ab
N2P0K2 147.83 ± 4.98bcd 2.37 ± 0.14abc 35.52 ± 2.04abc 88788.25 ± 5105.23abc
N2P1K2 153.28 ± 4.15bc 2.65 ± 0.22ab 39.70 ± 3.37ab 99241.25 ± 8432.10ab
N2P2K2 154.28 ± 6.50abc 2.78 ± 0.12a 41.66 ± 1.76a 104139.00 ± 4387.71a
N2P3K2 147.79 ± 3.30bcd 2.31 ± 0.02abc 34.70 ± 0.33abc 86.753.25 ± 818.68abc
N2P2K0 136.92 ± 4.10de 2.10 ± 0.11bc 31.53 ± 1.59bc 78.817.25 ± 3962.77bc
N2P2K1 161.23 ± 4.08ab 2.74 ± 0.16a 41.17 ± 2.37a 10.2934.88 ± 5916.02a
N2P2K3 146.67 ± 5.57bcd 2.53 ± 0.05abc 37.99 ± 0.76abc 94.978.75 ± 1907.91abc
N3P2K2 149.54 ± 6.55bcd 2.29 ± 0.05abc 34.33 ± 0.82abc 85.825.88 ± 2052.83abc
N1P1K2 156.65 ± 3.76abc 2.55 ± 0.14abc 38.23 ± 2.16abc 95.581.00 ± 5401.13abc
N1P2K1 142.12 ± 2.74cde 2.18 ± 0.15bc 32.72 ± 2.25bc 81.795.38 ± 5635.16bc
N2P1K1 167.42 ± 1.60a 2.85 ± 0.43a 42.72 ± 6.46a 106.803.63 ± 16,147.20a

Note: The data in the table represent mean ± standard deviation. Different letters following the numbers in the same column indicate significant differences at the 0.05 level.

The total yield of tomatoes under different fertilization treatments indicated that the yield was higher under fertilizer treatments than under non-fertilizer treatments. Among these, the highest yield was observed under the N2P1K1 treatment (106803.63 kg hm−2), followed by 104139.00 and 102934.88 kg hm−2 under the N2P2K2 and N2P2K1 treatments, respectively. These represented increases of 40.15, 36.66, and 35.08% compared with the non-fertilizer treatment. The interaction effect of nitrogen, phosphorus, and potassium was the strongest, followed by the interaction effects of phosphorus and potassium, and those of nitrogen and potassium, with yield increases of 20.70 and 16.51%, respectively (Table 6). The interaction effect of nitrogen and phosphorus was the weakest, with a yield increase of only 3.43%. The relative yield in the nitrogen-deficient areas was 88.32%, with a nitrogen dependence of 11.68%. The relative yield in phosphorus-deficient areas was 85.26%, with a phosphorus dependence of 14.74%. The relative yield in potassium-deficient areas was 75.68%, with a potassium dependence of 24.32%. Therefore, the order of dependence was as follows: nitrogen dependence < phosphorus dependence < potassium dependence, indicating that potassium fertilizer had a significant impact in terms of increasing yield compared with phosphorus fertilizer, whereas the effect of nitrogen fertilizer was smaller. Additionally, applying nitrogen, phosphorus, and potassium together was more conducive to increasing tomato yield.

Table 6

Effects of nitrogen, phosphorus, and potassium interaction on the tomato yield

Code Treatment Yield (kg hm−2) Increased production (%) Relative yield (%)
CK N0P0K0 76.204 73.18%
PK N0P2K2 91.980.75 20.70% 88.32%
NK N2P0K2 88.788.25 16.51% 85.26%
NP N2P2K0 78.817.25 3.43% 75.68%
NPK N2P2K2 104,139 36.66%

3.4 Effects of different nitrogen, phosphorus, and potassium ratios on the quality of tomato fruits

As depicted in Table 7, different ratios of nitrogen, phosphorus, and potassium fertilizer treatments significantly impacted the quality of tomato fruits. Among these, the N2P1K1 treatment exhibited the highest soluble protein content (3.86 mg g−1), followed by 3.54 mg g−1 under the N2P2K1 treatment. The soluble protein content under the N2P1K1 and N2P2K1 treatments was significantly higher than that under the no-fertilizer treatment, increasing by 69.30 and 55.26%, respectively.

Table 7

Effects of different nitrogen, phosphorus, and potassium ratios on tomato fruit quality

Treatment Soluble protein (mg g−1) Soluble sugar (%) Soluble solid (%) Vitamin C (mg 100 g−1) Organic acid (%) Nitrate (mg kg−1) Lycopene (mg kg−1)
N0P0K0 2.28 ± 0.10cd 3.05 ± 0.09c 4.17 ± 0.19cde 25.82 ± 1.31f 0.23 ± 0.05b 124.21 ± 0.89bcd 12.35 ± 0.42def
N0P2K2 2.32 ± 0.09cd 2.66 ± 0.04de 4.20 ± 0.06cde 31.03 ± 1.04ab 0.37 ± 0.12ab 110.09 ± 3.89d 10.98 ± 0.81f
N1P2K2 2.09 ± 0.32d 2.53 ± 0.15e 4.30 ± 0.10abcde 29.25 ± 0.49bcd 0.47 ± 0.12a 165.88 ± 33.20a 14.90 ± 1.18cde
N2P0K2 3.17 ± 0.04abcd 2.99 ± 0.22c 4.23 ± 0.09bcde 32.26 ± 0.63a 0.28 ± 0.08ab 119.96 ± 0.05bcd 11.44 ± 0.09ef
N2P1K2 2.41 ± 0.16cd 1.79 ± 0.06g 3.73 ± 0.17f 28.97 ± 0.41bcde 0.37 ± 0.05ab 171.78 ± 5.79a 12.87 ± 0.06def
N2P2K2 2.87 ± 0.58abcd 3.74 ± 0.05b 4.50 ± 0.06abc 31.30 ± 0.14ab 0.33 ± 0.02ab 118.64 ± 5.74bcd 15.56 ± 0.02bcd
N2P3K2 2.56 ± 0.49bcd 2.15 ± 0.03f 4.13 ± 0.03de 30.07 ± 0.96abc 0.26 ± 0.08ab 157.34 ± 1.68a 17.08 ± 1.11bc
N2P2K0 3.25 ± 0.32abc 3.62 ± 0.07b 4.43 ± 0.07abcd 28.15 ± 0.24cdef 0.33 ± 0.05ab 125.20 ± 1.14bcd 13.83 ± 1.06cdef
N2P2K1 3.54 ± 0.43ab 4.36 ± 0.11a 4.60 ± 0.15a 29.52 ± 0.58bcd 0.28 ± 0.08ab 112.41 ± 1.42cd 22.44 ± 2.04a
N2P2K3 2.38 ± 0.01cd 2.57 ± 0.19de 3.97 ± 0.09ef 30.07 ± 0.76abc 0.28 ± 0.00ab 162.63 ± 3.94a 15.16 ± 1.87cde
N3P2K2 2.54 ± 0.39bcd 2.45 ± 0.07ef 4.20 ± 0.06cde 27.19 ± 0.99def 0.37 ± 0.05ab 148.65 ± 0.86ab 14.33 ± 1.93cdef
N1P1K2 2.84 ± 0.22abcd 2.60 ± 0.03de 4.57 ± 0.07ab 27.60 ± 0.36cdef 0.33 ± 0.05ab 142.24 ± 1.60abc 18.98 ± 1.53b
N1P2K1 2.95 ± 0.54abcd 2.90 ± 0.03cd 4.20 ± 0.15cde 26.50 ± 1.19ef 0.37 ± 0.05ab 146.28 ± 1.02ab 12.32 ± 0.15def
N2P1K1 3.86 ± 0.41a 3.16 ± 0.12c 4.40 ± 0.06abcd 26.23 ± 0.99f 0.28 ± 0.00ab 166.87 ± 1.41a 16.68 ± 0.08bc

Note: The data in the table represent mean ± standard deviation. Different letters following the numbers in the same column indicate significant differences at the 0.05 level.

Significant differences were found in the soluble sugar content of tomato fruits under different treatments. At the same nitrogen and phosphorus levels, the soluble sugar content increased first and then decreased with increasing potassium fertilizer application. Among these, the N2P2K1 treatment exhibited the highest soluble sugar content (4.36%), followed by the N2P2K2 and N2P2K0 treatments, which were significantly higher than that under the CK treatment, with increases of 42.95, 22.62, and 18.69%, respectively. Conversely, the soluble sugar content was the lowest under the N2P1K2 treatment (1.79%).

The N2P2K1 treatment yielded the highest soluble solid content in tomato fruits, exhibiting significant differences compared with the N1P1K2 and CK treatments, with increases of 10.31 and 9.59%, respectively. Conversely, the lowest soluble solid content was recorded under the N2P1K2 treatment (3.73%), representing a decrease of 10.55% compared with that under the CK treatment. In addition, no significant differences were observed in the contents under the CK and other treatments.

The highest vitamin C content in tomato fruits was recorded under the N2P0K2 treatment (32.26 mg 100 g−1), compared with that under the CK treatment, with a 24.90% increase. Vitamin C content under the N2P2K0, N2P2K1, N2P2K2, and N2P2K3 treatments was 28.15, 29.52, 31.30, and 30.07 mg 100 g−1, respectively. The vitamin C content in tomatoes increased first and then decreased with the increase in potassium fertilizer application at the same nitrogen and phosphorus fertilizer levels. This suggested that, although increasing the potassium fertilizer amount increased the vitamin C content in fruits, excessive application should be avoided.

The organic acid content of tomatoes treated with N1P2K2 was the highest (0.47%), whereas the content of tomatoes treated with CK was the lowest (0.23%). A significant difference in content was observed between these two treatments. In addition, no significant difference was observed in the content between the N1P2K2 treatment and other treatments.

The nitrate content in tomato fruits was the highest under the N2P1K2 treatment (171.78 mg kg−1) compared with that under the CK treatment, with an increase of 38.30%. Conversely, the content was the lowest under the N0P2K2 treatment (110.09 mg kg−1), marking a decrease of 11.37% compared with that under the CK treatment. Under N1P2K2, N2P2K2, and N3P2K2 treatments, the nitrate content was 165.88, 118.64, and 148.65 mg kg−1, respectively, demonstrating increases of 50.68, 7.77, and 35.03%, respectively, compared with that under the N0P2K2 treatment, implying that additional nitrogen fertilizer application increased the nitrate content.

The combined application of nitrogen, phosphorus, and potassium fertilizers increased the lycopene content in fruits. The highest content was observed under the N2P2K1 treatment (22.44 mg kg−1), significantly surpassing that under other treatments. This represented an 81.70% increase compared with that under the non-fertilizer treatment. Moreover, at the same nitrogen and potassium fertilizer levels, the lycopene content increased with the increase in phosphorus fertilizer amount, peaking at 17.08 mg kg−1 under the N2P3K2 treatment, marking a 38.30% increase compared with that under the non-fertilizer treatment.

3.5 Fertilizer model and comprehensive evaluation analysis

3.5.1 Comprehensive analysis of the synergistic effects of nitrogen, phosphorus, and potassium fertilizers

Based on the “3414” field fertilizer experimental design, regression analysis was conducted on the interaction effects between nitrogen, phosphorus, and potassium using the fertilization and yield data from the 14 treatment groups. Then, a ternary fertilizer effect function equation was fitted.

Y = – 0.543 N ² – 2.843 P ² – 0.094 K ² + 385.613 N – 270.732 P + 40.725 K – 0.214 N P – 0.460 N K + 1.526 P K + 75411.899 .

According to the ternary fertilizer effect equation, the maximum application amount of nitrogen, phosphorus, and potassium was 140.49, 73.33, and 472.26 kg hm−2, respectively. The calculated theoretical maximum yield was 102118.84 kg hm−2. When the levels of nitrogen, phosphorus, and potassium were all 0, the constant term (theoretical blank area yield) was 75411.899 kg hm−2, which closely matched the actual yield of 76204.00 kg hm−2. This indicated that the fitting of the fertilizer effect function equation was relatively consistent with production reality, providing an objective reflection of the impact of combined fertilizer application on yield and fertilizer effects.

3.5.2 Comprehensive evaluation and analysis of tomato quality and yield

A relatively optimal fertilizer ratio was selected using principal component analysis for comprehensive analysis. The soluble sugar content, soluble protein content, soluble solid content, organic acid content, sugar acid ratio, vitamin C content, nitrate content, tomato lycopene content, and total yield of 14 tomato treatment groups were comprehensively evaluated to select a fertilizer ratio that would produce high-quality and high-yield tomatoes. First, the original data were standardized, and then the standardized values were analyzed to obtain the eigenvalues and contribution rates of the correlation matrix (Table 8). According to the principle that eigenvalues greater than 1 can be extracted, four principal components were identified with eigenvalues of 3.54, 1.85, 1.33, and 1.15 and variance contribution rates of 39.33, 20.53, 14.76, and 12.81%, respectively.

Table 8

Eigenvalues and variance contribution rates of principal component analysis

Component Total Initial eigenvalues Total Sum of squared loads
Percent variance Accumulation (%) Percent variance Accumulation (%)
1 3.54 39.33 39.33 3.54 39.33 39.33
2 1.85 20.53 59.85 1.85 20.53 59.85
3 1.33 14.76 74.61 1.33 14.76 74.61
4 1.15 12.81 87.42 1.15 12.81 87.42
5 0.57 6.28 93.70
6 0.23 2.54 96.24
7 0.17 1.88 98.12
8 0.09 0.99 99.11
9 0.08 0.89 100.00

F 1–F 4 represent the scores of the four principal components, and the score coefficient equations for the four principal components are as follows:

F 1 = 0.4661 X 1 + 0.3922 X 2 + 0.4235 X 3 − 0.2976 X 4 + 0.3661 X 5 + 0.0409 X 6 − 0.3353 X 7 + 0.3189 X 8 + 0.1058 X 9 ,

F 2 = 0.0375 X 1 + 0.1905 X 2 + 0.217 X 3 + 0.3788 X 4 − 0.3964 X 5 − 0.0427 X 6 + 0.3376 X 7 + 0.3994 X 8 + 0.5825 X 9 ,

F 3 = 0.1336 X 1 − 0.2134 X 2 + 0.0833 X 3 + 0.3522 X 4 − 0.105 X 5 + 0.7244 X 6 − 0.4399 X 7 − 0.2091 X 8 + 0.1805 X 9 ,

F 4 = − 0.2785 X 1 − 0.0699 X 2 − 0.3502 X 3 − 0.4201 X 4 + 0.353 X 5 + 0.4546 X 6 + 0.2599 X 7 + 0.2431 X 8 + 0.4006 X 9 .

The comprehensive score for each treatment was calculated based on the values of F 1, F 2, F 3, and F 4, as well as the weights of the contributions of each principal component.

F = 0.4498 F 1 + 0.2348 F 2 + 0.1689 F 3 + 0.1465 F 4 .

The nutritional quality and yield of 14 tomato treatments were comprehensively evaluated based on the F value. As shown in Table 9, the treatment with the highest comprehensive score was N2P2K1, followed by N2P2K2.

Table 9

Comprehensive score table of tomato nutrition quality and yield with different NPK ratios

Treatment F 1 F 2 F 3 F 4 F Ranking
N0P0K0 0.32 –3.03 –1.43 –0.47 –0.88 12
N0P2K2 –0.69 –1.12 2.09 –0.18 –0.25 8
N1P2K2 –2.28 1.98 0.98 –0.64 –0.49 10
N2P0K2 0.90 –1.65 1.36 0.74 0.35 5
N2P1K2 –3.46 0.79 –0.17 1.06 –1.24 14
N2P2K2 1.58 0.90 1.86 -0.04 1.23 2
N2P3K2 –0.20 –1.05 –0.78 1.93 –0.18 7
N2P2K0 1.03 –0.70 –0.05 –1.81 0.03 6
N2P2K1 4.43 1.01 0.21 0.46 2.33 1
N2P2K3 –1.23 –0.12 –0.20 1.56 –0.39 9
N3P2K2 –1.52 0.19 –0.51 –0.98 –0.87 11
N1P1K2 0.74 1.18 –0.64 –0.26 0.47 4
N1P2K1 –1.09 –0.19 –0.74 –1.74 –0.92 13
N2P1K1 1.47 1.81 –2.00 0.38 0.80 3

4 Discussion

Soil serves as the essential substrate for crop survival, with nitrogen, phosphorus, and potassium elements providing the nutrients necessary for plant growth and development. The physical and chemical properties of soil serve as crucial indicators for evaluating soil quality, directly reflecting its vitality and the status of nutrient supply. Excessive fertilization can decrease soil pH and increase EC values under experimental conditions, leading to soil acidification and increased soil salinity. This situation is not conducive to plant growth and development. The contents of soil alkali-hydrolyzable nitrogen, available phosphorus, and available potassium increase with the application of increased amounts of nitrogen, phosphorus, and potassium fertilizers. This may be attributed to the incomplete absorption of fertilizers by plants. This not only hampers the tomato fruit yield and quality but also contributes to soil salinization and fertilizer wastage, consistent with the findings of previous studies [19].

Nitrogen, phosphorus, and potassium are essential for plant growth. Plant height and stem thickness serve as crucial indicators of tomato nutrition and growth. In this study, plants treated with N0P2K2 exhibited the largest plant height, whereas those treated with N2P0K2 had the largest leaf area. This suggested that the coordination between phosphorus and potassium led to taller plants without nitrogen fertilizer, and the coordination between nitrogen and potassium resulted in larger leaf areas without phosphorus fertilizer. This could be due to sufficient nitrogen in the soil, supporting the early growth of tomato plants. Plants treated with N2P1K1 displayed the largest stem thickness, whereas those treated with N2P3K2 had the smallest stem thickness. This indicated that excessive phosphorus fertilizer might not be conducive to the increase in stem thickness of tomatoes. Within a certain range, the application of nitrogen, phosphorus, and potassium fertilizers could promote plant growth. However, excessive amounts of any nutrient led to nutrient imbalance, resulting in no growth-promoting effect or even growth inhibition [20]. Studies showed that an appropriate combination of nitrogen, phosphorus, and potassium fertilizers enhanced dry matter accumulation in potato tubers [21]. Similarly, this study revealed that plants treated with N2P1K1 exhibited the highest dry matter accumulation, and the interaction between nitrogen, phosphorus, and potassium significantly affected dry matter accumulation in tomatoes.

The soluble protein, soluble sugar, soluble solids, organic acids, vitamin C, and lycopene in tomato fruits are vital quality indicators that directly influence the nutritional value, taste, and commercial value of tomatoes [22,23]. The quality of tomato fruits is influenced by various ratios of nitrogen, phosphorus, and potassium, and an optimal ratio can significantly improve it [24,25,26]. This study showed that an appropriate combination of nitrogen, phosphorus, and potassium effectively improved the nutritional quality of tomato fruits. Additionally, potassium fertilizer application increased the contents of soluble sugar, soluble solids, soluble protein, and lycopene in tomato fruits to varying degrees. These contents first increased and then decreased with the increase in potassium application, which was consistent with previous research findings [27]. Additionally, excessive use of nitrogen fertilizer and prolonged application significantly elevated the nitrate content in tomato fruits, exceeding the standard limit and posing health risks to consumers.

Fertilization plays a crucial role in boosting the tomato yield. Proper fertilization practices are essential for vegetable crops to achieve high yields and maintain quality standards [28]. In the experimental setup, the N2P1K1 treatment led to the highest yield, with the amount of phosphorus and potassium fertilizers being below the optimal level. The impact of nitrogen, phosphorus, and potassium fertilizers on the tomato yield follows the following order: nitrogen < phosphorus < potassium, suggesting that increasing potassium fertilizer application could effectively increase the tomato yield.

Some studies showed that the pumpkin yield increased first and then decreased with the increase in the amount of nitrogen, phosphorus, and potassium fertilizers, with recommended dosages of 390.5 kg for nitrogen fertilizer, 213.8 kg for phosphorus fertilizer, and 371.3 kg for potassium fertilizer per hectare for “Jianbao” pumpkin [29]. Similarly, studies on Angelica sinensis suggested the optimal fertilizer application amounts of nitrogen, phosphorus, and potassium fertilizers to be 81.15, 80.67, and 29.78 kg hm−2, respectively, resulting in a maximum seedling transplanting yield of 1932.52 kg hm−2 [15]. The ternary quadratic fertilizer function equation fitted in this “3414” fertilizer experiment expressed the relationship between the total tomato yield and the application of nitrogen, phosphorus, and potassium fertilizers. Based on this equation, the calculated application rate was 140.49 kg hm−2 for nitrogen fertilizer, 73.33 kg hm−2 for phosphorus fertilizer, and 472.26 kg hm−2 for potassium fertilizer, with a fertilizer ratio of 1:0.50:3.05. The highest expected yield was projected to be 102118.84 kg hm−2.

5 Conclusions

Fertilization significantly impacts both the quality and yield of tomatoes. Applying nitrogen, phosphorus, and potassium fertilizers in various proportions can enhance soil fertility, stimulate tomato plant growth, and augment both the quality and yield of tomatoes. A comprehensive analysis revealed that the most suitable fertilization treatment under the experimental environment and planting conditions was N2P2K1. Therefore, N2P2K1 was recommended as the optimal fertilization formula for producing high-quality and high-yield tomatoes in the experimental area, with the amount of nitrogen fertilizer being 197.28 kg hm−2, phosphorus fertilizer being 88.75 kg hm−2, and potassium fertilizer being 229.80 kg hm−2. This approach was conducive to achieving efficient and high-quality tomato production. However, the yield and quality of tomatoes were affected by various factors besides fertilization, necessitating further studies on other aspects.

Acknowledgements

This study was supported by the Jilin Province Science and Technology Development Plan Project (20210202123NC).

  1. Funding information: This study was supported by the Jilin Province Science and Technology Development Plan Project (20210202123NC).

  2. Author contributions: C.W.: experimental design, execution, and writing first draft. X.H.: experimental design, execution, and data analysis. Y.C.: experimental design, data analysis, and manuscript writing and revision. X.W.: experimental design and data analysis. W.W.: experimental design and manuscript writing and revision.

  3. Conflict of interest: Authors state no conflict of interest.

  4. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Received: 2024-03-19
Revised: 2024-05-10
Accepted: 2024-05-17
Published Online: 2024-06-28

© 2024 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  187. Exploring the coupling mode of water and fertilizer for improving growth, fruit quality, and yield of the pear in the arid region
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  205. Erratum
  206. Erratum to “Investigating the association between dietary patterns and glycemic control among children and adolescents with T1DM”
  207. Erratum to “Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation”
  208. Retraction
  209. Retraction to “MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB”
  210. Retraction to “A data mining technique for detecting malignant mesothelioma cancer using multiple regression analysis”
  211. Special Issue on Advances in Neurodegenerative Disease Research and Treatment
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  213. Special Issue on Multi-omics
  214. Inflammasome complex genes with clinical relevance suggest potential as therapeutic targets for anti-tumor drugs in clear cell renal cell carcinoma
  215. Gastroesophageal varices in primary biliary cholangitis with anti-centromere antibody positivity: Early onset?
https://doi.org/10.1515/biol-2022-0893
Keywords for this article
“3414” experimental design; quality; tomato; yield
Creative Commons
BY 4.0

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  207. Erratum to “Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation”
  208. Retraction
  209. Retraction to “MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB”
  210. Retraction to “A data mining technique for detecting malignant mesothelioma cancer using multiple regression analysis”
  211. Special Issue on Advances in Neurodegenerative Disease Research and Treatment
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