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Helicobacter pylori causes gastric dysbacteriosis in chronic gastritis patients

  • Chao Cen , Qiuying Du , Bin Luo , Tonghua Wang , Jianwei Su , Xiaoshan Qin , Wenyan Zhang , Lijing Lu , Yang Liao , Yanqiang Huang and Yumei Liang EMAIL logo
Published/Copyright: March 28, 2024
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Open Life Sciences
From the journal Open Life Sciences Volume 19 Issue 1

Abstract

Gastric mucosal samples were procured and underwent the sequencing of 16S ribosomal RNA (16S rRNA) via Illumina high-throughput sequencing technology to explore the impact of Helicobacter pylori (H. pylori) infection on the composition of gastric flora in chronic gastritis (CG) patients. In the results, the operational taxonomic unit (OTU) analysis revealed an overlap of 5706 OTUs shared between the two groups. The top 5 abundance ranking (TOP5) phyla comprised Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Epsilonbacteraeota, while the TOP5 genus was Lachnospiraceae_NK4A136_group, Helicobacter, Bacteroides, Klebsiella, and Pseudomonas. In the metabolic pathways at the Kyoto Encyclopedia of Genes and Genomes (KEGG)_L3 level, conspicuous variations across seven functions were observed between the H. pylori-positive (HP_Pos) and H. pylori-negative (HP_Neg) groups. Subsequently, functional gene enrichment in KEGG pathways was further validated through animal experimentation. In contrast to the mice in the HP_Neg group, those infected with H. pylori manifested an infiltration of inflammatory cells, an augmentation in gastric acid secretion, and conspicuously elevated scores regarding gastric activity, along with heightened levels of malondialdehyde. In conclusion, CG patients infected with H. pylori displayed a disorder in gastric flora, furnishing a theoretical basis for the prophylaxis of H. pylori infection and its associated pathogenic ramifications.

Keywords: chronic gastritis; Helicobacter pylori ; 16S ribosomal RNA; operational taxonomic unit; Kyoto encyclopedia of genes and genomes

1 Introduction

Chronic gastritis (CG) manifests as a malady characterized by persistent inflammatory or atrophic lesions within the gastric mucosa, holding a preeminent position in terms of prevalence among various gastric disorders [1]. The principal prototypes of CG include chronic superficial gastritis (CSG), chronic erosive gastritis, and chronic atrophic gastritis (CAG). Amidst the phase of CAG, patients become notably susceptible to gastric cancer, a reality acknowledged by the World Health Organization’s classification of CAG as a precancerous state in the evolution of gastric cancer [2]. Nonetheless, the majority of these alterations can be reversed during the CSG phase, thereby emphasizing the critical importance of prompt and efficacious management of CG.

The onset of CG is initiated by diverse etiological factors, encompassing elements such as Helicobacter pylori (H. pylori) infection [3], autoimmunity [4], and pharmaceutical influences [5]. Of these, H. pylori infection constitutes approximately 90%, emerging as the predominant etiology of CG [6]. H. pylori, a spiral-shaped Gram-negative bacterium, was initially isolated from the gastric mucosa of patients with CG in the 1980s and cultivated in a microaerobic environment, indicating a robust association between H. pylori and CG [7]. Upon adhering to the gastric mucosa, H. pylori injects virulence factors into the gastric epithelial cells, inciting chronic inflammatory and immune responses, which gradually inflicts damage upon the gastric mucosa and further exacerbates gastric tissue damage [8]. Nevertheless, the precise pathogenic mechanism through which H. pylori infection gives rise to CG remains ambiguous, warranting further in-depth investigation.

In recent years, the cultivation and high-throughput sequencing of gastric juice and biopsies have unveiled the intricate bacterial microbiota inhabiting the human stomach [9]. The gastric microorganisms predominantly encompass Proteobacteria, Phylum Firmicutes, Bacteroidetes, and Actinobacteria, assuming crucial roles in digestion, absorption, metabolism, immunity, and the restraint of pathogen colonization [10]. They maintain the ecological equilibrium in the stomach, and any structural or functional imbalance among them has the potential to precipitate various maladies [11]. The diversity of microorganisms varies with the health status of the gastric mucosal epithelium, with an inverse correlation manifesting between H. pylori and the diversity of the gastric microbiota [12]. The colonization of H. pylori results in a reduction in bacterial diversity, profoundly disrupting the microecological environment in the stomach, thus leading to various gastric maladies and ultimately impacting the health of the host [13]. Nevertheless, the extent to which H. pylori, a pivotal pathogenic bacterium in CG, is associated with the alterations in the gastric microbiota of CG patients remains a domain that has not yet undergone comprehensive exploration.

In this investigation, specimens were procured from the gastric mucosa of H. pylori-positive (HP_Pos) and H. pylori-negative (HP_Neg) CG patients. The differences in bacterial communities were examined employing 16S ribosomal RNA (16S rRNA) sequencing technology to delve into the impact of H. pylori infection on the composition of the gastric flora. Additionally, this study unveiled the underlying pathogenic mechanism of H. pylori infection through the further animal experiments. Such revelations establish a theoretical basis for the subsequent endeavor of pursuing novel remedies targeting the restoration of the microecological balance of gastric mucosa, as well as for the prevention of H. pylori infection and its associated pathogenic ramifications.

2 Materials and methods

2.1 Patients and samples

This study investigated and screened both outpatients and inpatients exhibiting upper gastrointestinal symptoms who underwent gastroscopy at the Endoscope Center of the Affiliated Hospital of Youjiang Medical University for Nationalities between June 2020 and November 2020. This study completed registration in the Chinese clinical trial registry (registration website: https://www.chictr.org.cn/index.html). The research received approval from the Medical Ethics Review Committee of the Affiliated Hospital of Youjiang Medical University for Nationalities (approval number: YYFY-LL-2017-01), and the written informed consent was obtained from all participating patients.

Exclusion criteria were as follows: (1) individuals who had taken proton pump inhibitors, H2 receptor antagonists, or other antacids, probiotics, mucosal protective agents, or antibiotics within the preceding 4 weeks (as all these medications directly impact the gastric mucosa flora); (2) a history of gastric adenoma, gastric cancer, or mucosa-associated lymphoid tissue lymphoma (all manifesting as alterations in the physiological structure of the gastric mucosa); (3) individuals who had undergone gastrectomy (as most of these patients have a history of advanced gastric cancer, leading to changes in the normal physiological structure of the stomach); and (4) patients who had undergone H. pylori eradication but were again found to be H. pylori positive [14].

Patients with upper gastrointestinal symptoms, diagnosed through gastroscopy and pathology as CG, and tested for H. pylori were included in this study. CG patients participating in the study underwent the 13C urea breath test (UBT), rapid urease test (RUT), and pathological histology staining of the gastric mucosa. H. pylori infection is defined as a positive result for pathological histology of the gastric mucosa, coupled with a positive outcome in either of the following two tests: (1) a UBT positive result exhibiting delta over baseline greater than 4.0% or (2) a positive RUT result. Furthermore, according to prior research on the gastric microbiome, samples with less than 1% relative abundance of H. pylori were excluded from the analysis to ensure higher representativeness [15].

  1. Informed consent: Informed consent has been obtained from all individuals included in this study.

  2. Ethical approval: The research related to human use has been complied with all the relevant national regulations, institutional policies and in accordance with the tenets of the Helsinki Declaration, and has been approved by the Medical Ethics Review Committee of the Affiliated Hospital of Youjiang Medical University for Nationalities (approval number: YYFY-LL-2017-01).

2.2 Animals

Twenty-four male C57BL/6 mice (20–25 g) aged 6–8 weeks were purchased from Sipeifu Biotechnology Co., Ltd. (Beijing, China). These animals were kept in a specific pathogen-free condition with a 12-h light/dark cycle, a room temperature of 22 ± 2°C, humidity between 50 and 60%, and free access to both food and drinking water. Approval for all experimental procedures was obtained from the Medical Ethics Review Committee of the Affiliated Hospital of Youjiang Medical University for Nationalities (approval number: YYFY-LL-2017-01), adhering to the National Research Council’s Guide for the Care and Use of Laboratory Animals.

  1. Ethical approval: The research related to animal use has been complied with all the relevant national regulations and institutional policies for the care and use of animals and has been approved by the Medical Ethics Review Committee of Youjiang Medical University for Nationalities (approval number: YYFY-LL-2017-01).

2.3 Collection of sequencing sample

The gastric body stands as the prevalent site for autoimmune gastritis, whereas the gastric sinus represents the most frequent site of atrophic gastritis. In this investigation, a specimen was obtained from the gastric body, and the other specimen was acquired from the gastric antrum, and the two samples underwent combined sequencing. The specific procedural steps were as follows: one piece of gastric sinus mucosa and one piece of gastric body mucosa were extracted under gastroscopy utilizing disposable sterile biopsy forceps and then loaded into sterile sealed 5 ml lyophilized tubes containing 2 ml of sterile saline. Following labeling the tubes and recording the corresponding specimen information, the tubes were promptly placed in a thermos flask containing liquid nitrogen to arrest the intrinsic enzyme activity and oxidation. Subsequently, the tubes were transported to the laboratory of the Experimental Center of the Youjiang Medical University for Nationalities within 1 h for freezing and stored frozen at −80°C. These samples were later employed for high-throughput sequencing of 16S rRNA.

2.4 Sequencing process

Genomic DNA was extracted from 26 gastric mucosa samples utilizing a DNA extraction kit following the manufacturer’s protocols, and the purity and concentration of the DNA were assessed through 1.2% agarose gel electrophoresis. Employing the diluted genomic DNA as a template, PCR amplification of the target fragment (bacterial diversity identification region: 16S V3-V4 region) was conducted with specific primers designed based on the conservative regions in the sequence with high-fidelity enzymes. Post-electrophoretic detection, the PCR amplification products underwent purification with magnetic beads and served as templates for the second-round PCR. Subsequently, the second-round PCR amplification, electrophoresis, and purification were conducted. The final PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit and subjected to sequencing on the machine after passing the quality control. The PCR primers are detailed in Table 1.

Table 1

PCR primers

Sequencing Primer Primer sequence Region
Bacteria 343F 5′-TACGGRAGGCAGCAG-3′ V3–V4
16S rRNA 798R 5′-AGGGTATCTAATCCT-3′

2.5 Bioinformatics analysis

The Illumina Miseq sequencing platform has the ability to concurrently sequence 16S rRNA genes from multiple samples and has been extensively employed for the investigation of the evolution and diversity among microbial species. Following quality filtration, elimination of chimeric sequence and the final sequence were used for operational taxonomic unit (OTU) clustering. In this process, parameters with sequence similarity greater than or equal to 97% were grouped into one OTU unit, and all representative sequences were annotated against the database [16]. Various analyses were performed, including OTU clustering analysis, alpha diversity analysis, beta diversity analysis, community composition analysis, species difference analysis, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) functional prediction analysis.

2.6 Quantitative real-time PCR (qRT-PCR)

qPCRs were conducted using the FTC-3000TM Real-Time Quantitative Thermal Cycler (Funglyn, Shanghai, China). All qPCRs were performed with three replicates per DNA sample. Standard curves were established by serially diluting the plasmid of a pMD18-T vector containing the appropriate insert from 107 to 1012 target gene copies per μl for each primer set. The standard curve was generated using linear regression of threshold cycle numbers (Ct) versus log copy numbers of targets.

The qPCRs were executed in 25 μl of reaction mixtures composed of 12.5 μl SRBR Premix Ex Taq™ (2×) (Takara, Japan), 1 μl (10 μM) of each forward and reverse primers (357F and 806R), 5 μl of template DNA, and sterilized deionized water. The reaction conditions were as follows: pre-denaturation at 95°C for 30 s, denaturation at 95°C for 10 s, annealing at 55°C for 30 s, extension at 72°C for 30 s (40 cycles), and a final extension at 72°C.

2.7 Establishing the model of H. pylori infection in mice

Subsequent to a 1-week acclimatization period, the mice were arbitrarily apportioned into two cohorts, each comprising 12 mice. The establishment of the animal model followed the patent of Dr. Yanqiang Huang (Patent No. ZL201910245354.5) [17]. Mice in the HP_Pos group underwent gavage with a clinically isolated H. pylori strain, diluted to a suspension of 1 × 109 CFU/ml. This administration involved a volume of 0.5 ml per rodent, administered once every 2 days for a total of five instances. Fasting and water deprivation for 12 h before gavage, and fasting and water deprivation for 4 h after gavage. In the meantime, the mice in the HP_Neg group received an equivalent volume of physiological saline.

Extensive pre-experimental studies conducted by our team confirmed that the mouse H. pylori model, constructed as per the present invention, achieves a success rate of 90% within 3 months [17]. At the 6- and 12-week intervals following the final gavage, six mice from each group were randomly selected for sacrifice through cervical dislocation, respectively. Gastric tissues of the mice were expeditiously harvested for routine hematoxylin–eosin (H&E) staining and pathological scrutiny. Moreover, gastric juice was collected and underwent volumetric quantification and concentrations of acid were determined through titration.

2.8 H&E staining

The stomachs of the mice underwent immersion in a 10% neutral buffered formalin solution for fixation. Subsequent to this procedure, a strip extending from the gastric sinus to the gastric body was collected and embedded in paraffin. Then, the strips were sectioned into 4 μm pieces, followed by routine H&E staining. The images were captured utilizing a microscope.

2.9 Enzyme-linked immunosorbent assay (ELISA)

The determination of gastric malondialdehyde (MDA) concentration was carried out employing the thiobarbituric acid (TBA) reactive substances assay kit (Solarbio, Beijing). The underlying principle involves the reaction between one molecule of MDA and two molecules of TBA, yielding a red MDA-TBA complex under high-temperature (90–100°C) and acidic conditions, which can be quantitated using a spectrophotometer at 532 nm. The assay procedures were executed as described. The concentration of MDA was expressed in terms of nmol/mg protein.

2.10 Statistical analysis

Alpha diversity analysis was conducted utilizing the Mann–Whitney U-test. For the assessment of beta diversity, two- and three-dimensional principal co-ordinate analysis (PCoA) plots were generated based on the branch length of the phylogenetic tree shared between the groups. The Wilcoxon rank-sum test was employed to carry out the analysis of distinctions between the two groups of species. Independent sample t-tests were employed for the inter-group comparison of measurement data, and χ2 tests were applied for inter-group comparisons of counting data using SPSS for Windows, version 20. All P-values were bilateral, and a P-value of < 0.05 was considered statistically significant.

3 Results

3.1 Characteristics of the patients

Twelve H. pylori-positive CG patients and 14 H. pylori-negative CG patients were included in this study. The age of the enrolled patients ranged from 37 to 84 years old, and there was no noteworthy distinction in terms of age and gender between the two groups (Table 2).

Table 2

Comparison of baseline characteristics (mean  ±  SD)

Characteristics HP_Pos group (n = 12) HP_Neg group (n = 14) t/χ2 value P value
Age (years) 49.33 ± 16.44 53.00 ± 11.27 0.671 0.508
Gender ( n , %)
Male 5 (41.67) 7 (50) 0.154 0.695
Female 7 (58.33) 7 (50)

HP_Pos: H. pylori positive; HP_Neg: H. pylori negative.

3.2 OTU clustering analysis

A total of 131,981 valid sequences were acquired from 26 samples utilizing Illumina high-throughput sequencing technology. Tags were annotated at each taxonomic level, that is, the aggregate count of tags corresponding to OTUs at each taxonomic level of the kingdom, phylum, class, order, family, genus, and species were summarized to generate an OTU level barplot illustrating the annotation proportion of each sample (Figure 1a).

Figure 1 OTU clustering analysis of gastric mucosa microflora. (a) OTU level barplot; (b) Venn diagram demonstrates the shared and unique OTUs in both groups; and (c) the flower petal diagram of OTUs in both groups.
Figure 1

OTU clustering analysis of gastric mucosa microflora. (a) OTU level barplot; (b) Venn diagram demonstrates the shared and unique OTUs in both groups; and (c) the flower petal diagram of OTUs in both groups.

OTU clustering, executed with a 97% similarity threshold, yielded a total of 11,731 OTUs. The Venn diagram generated by R software revealed that the HP_Pos group possessed 8,412 OTUs, while the HP_Neg group obtained 9,025 OTUs. The un-overlapped portion denoted the number of OTUs unique to each group, with the HP_Neg group exhibiting 613 more unique OTUs than the HP_Pos group. Notably, 5,706 OTUs were shared by the two groups, signifying a considerable degree of similarity in the bacterial structure between the two groups (Figure 1b).

Based on the results of the OTU clustering analysis, sample optimization of each group was conducted, and the overlap between each sample was displayed in the form of petals to elucidate the quantity of OTUs per sample and the cumulative OTUs count across all samples. The flower petal diagram conveyed that the overall number of OTUs in all samples was 151 (Figure 1c).

3.3 Alpha diversity analysis

Alpha diversity analysis was employed to assess the abundance and diversity of microorganisms in the samples. Various indices, including Observed_species (Figure 2a), Chao1 (Figure 2b), Shannon (Figure 2c), Simpson (Figure 2d), PD_whole_tree (Figure 2e), and goods_coverage (Figure 2f), were employed to construct the corresponding exponential sparse curves at the OTU level for all samples. These curves served to evaluate the adequacy and rationality of the sequencing amount of samples and reflect the species richness of the samples. The dilution curve demonstrated a tendency to plateau, indicating that the sequencing volume saturation of the samples in both groups was sufficient, and the bacterial species richness, species coverage, and sample uniformity were reasonable. The specaccum species accumulation curve (Figure S1a), generated by R software, displayed a diminishing increase rate, leveling off with the expansion of the sequencing sample size. This observation suggested that the sample size covered most bacterial communities and could reflect the species richness. The gentle ascent of the curve implied that species in this environment would not substantially increase with the increase in sample size, indicating that the sampling was sufficient. The rank abundance curve (Figure S1b) further revealed that the majority of samples in the two groups displayed wide and flat horizontal curves with a gentle broken line, indicating that the species richness and evenness of most samples were reasonable, and the sequencing quantity could cover the majority of bacterial species, which was sufficient for subsequent analyses.

Figure 2 Alpha diversity analysis of gastric mucosa microflora. (a) Observed species curve; (b) Chao1 curve; (c) Shannon curve; (d) Simpson curve; (e) PD dilution curve; and (f) good coverage curve.
Figure 2

Alpha diversity analysis of gastric mucosa microflora. (a) Observed species curve; (b) Chao1 curve; (c) Shannon curve; (d) Simpson curve; (e) PD dilution curve; and (f) good coverage curve.

Based on the calculation of the diversity indices across different samples at unified depth, the violin plots of the diversity indices for different groups were generated to depict the distribution of the above six diversity indices in the two groups and to visually assess the degree of sample dispersion and the high and low differences of the indices between the two groups (Figure S2). According to statistical analysis, although there were differences in the alpha diversity index between the groups, the differences were not statistically significant, reflecting that there was no substantial distinction in the community richness and diversity between the samples of the HP_Pos group and the HP_Neg group (Table 3).

Table 3

Comparative analysis of α diversity index between the HP_Pos group and the HP_Neg group

α diversity index HP_Pos group HP_Neg group P value
Observed_species 1555.87 ± 181.16 1674.04 ± 200.41 0.23
Chao1 1793.42 ± 274.637 1951.36 ± 312.69 0.43
Shannon 8.913 ± 0.43 8.93 ± 0.72 0.27
Simpson 0.99 ± 0.01 0.99 ± 0.01 0.40
PD_whole_tree 72.39 ± 5.38 75.66 ± 5.12 0.16
Goods_coverage 0.98 ± 0.01 0.978 ± 0.01 0.40

HP_Pos: H. pylori positive; HP_Neg: H. pylori negative.

3.4 Beta diversity analysis

Beta diversity serves as an indicator of the diversity disparity among different samples and gauges the similarity in microbial composition between individuals. Consequently, the PCoA 2D (Figure 3a and b) and PCoA 3D (Figure 3c and d) were employed to investigate the distinctions in β diversity between the samples of the two groups. The outcomes indicated that neither unweighted uniFrac nor weighted uniFrac PCoA could effectively distinguish the gastric mucosal bacterial community composition between the HP_Pos group and the HP_Neg group; there was clustering and overlap, with no statistical difference in β diversity (P > 0.05).

Figure 3 Beta diversity analysis of gastric mucosa microflora. PCoA plot (2D features) with (a) unweighted uniFrac and (b) weighted uniFrac distance metrics; PCoA plot (3D features) with (c) unweighted uniFrac and (d) weighted uniFrac distance metrics.
Figure 3

Beta diversity analysis of gastric mucosa microflora. PCoA plot (2D features) with (a) unweighted uniFrac and (b) weighted uniFrac distance metrics; PCoA plot (3D features) with (c) unweighted uniFrac and (d) weighted uniFrac distance metrics.

3.5 Distribution of community structure

According to the taxonomic level of phylum, class, order, family, genus, and species, the top 15 abundance ranking (TOP15) species were plotted as histograms. The x-axis represented the sample, presenting the proportion of each species in each sample. Additionally, heatmaps were generated based on the abundance of species in the TOP15 species of each sample at various taxonomic levels by clustering taxonomic information and inter-sample variation to visually compare the species abundance at different taxonomic levels in each sample.

The community composition of all samples and samples between the groups were analyzed at the phylum level (Figure 4). The results revealed that the gastric mucosal flora in both two groups was primarily distributed in nearly 15 phyla. Among them, Bacteroidetes (37.92%) exhibited the highest bacterial abundance in the stomach of H. pylori-positive CG patients, followed by Proteobacteria (23.78%), Firmicutes (23.46%), Actinobacteria (6.20%), and Epsilonbacteraeota (4.95%). Similarly, the TOP5 dominant phyla in the intragastric flora of H. pylori-negative CG patients were consistent with those of H. pylori-positive CG patients, albeit with varying proportions, which were Bacteroidetes (39.08%), Proteobacteria (25.96%), Firmicutes (24.35%), Actinobacteria (4.55%), and Epsilonbacteraeota (2.83%), respectively. Regarding inter-group comparisons, the proportions of Bacteroidetes (P = 0.95), Proteobacteria (P = 0.80), and Firmicutes (P = 0.99) in the HP_Neg group were higher than those in the HP_Pos group, with no significant difference; while the proportions of Actinobacteria (P = 0.24) and Epsilonbacteraeota (P < 0.05) in the HP_Neg group were lower than those in the HP_Pos group.

Figure 4 Species distribution at the phylum level. (a) Histogram of the relative abundance of bacteria in gastric mucosa of all samples; (b) histogram of the relative abundance of bacteria in gastric mucosa of two groups; and (c) the TOP15 phylum level abundance cluster diagram of all samples.
Figure 4

Species distribution at the phylum level. (a) Histogram of the relative abundance of bacteria in gastric mucosa of all samples; (b) histogram of the relative abundance of bacteria in gastric mucosa of two groups; and (c) the TOP15 phylum level abundance cluster diagram of all samples.

At the genus level, there was no significant difference in the composition map of gastric flora between the two groups (Figure 5). In the HP_Pos group, Lachnospiraceae_NK4A136_group exhibited the highest abundance, accounting for about 17.63%, followed by Helicobacter (15.24%), Bacteroides (11.22%), Klebsiella (8.27%), and Pseudomonas (6.12%), respectively. Moreover, there were 10 genera constituted more than 1%, including Alistipes and Escherichia-shigella. The results of community distribution in the HP_Neg group revealed that Lachnospiraceae_NK4A136_group was also the most abundant genus, accounting for a high percentage of 18.73%, followed by Pseudomonas (14.30%), Klebsiella (9.97%), Bacteroides (9.17%), and Helicobacter (8.13%). The proportion of dominant bacteria exhibited variance between the two groups. In the stomach of H. pylori-positive CG patients, Helicobacter emerged as the second dominant bacteria (P < 0.05), and the proportion of Pseudomonas was remarkably reduced compared to H. pylori-negative CG patients (P < 0.05). The content of the representative strains, namely L. multiparus, H. pylori, B. fragilis, K. peneumoniae, and P. aeruginosa, belonging to the TOP5 genus in the samples of both groups was further verified through qRT-PCR, and the obtained results were in agreement with the aforementioned findings (Figure 6).

Figure 5 Species distribution at the genus level. (a) Histogram of the relative abundance of bacteria in gastric mucosa of all samples; (b) histogram of the relative abundance of bacteria in gastric mucosa of two groups; and (c) the TOP15 genus level abundance cluster diagram of all samples.
Figure 5

Species distribution at the genus level. (a) Histogram of the relative abundance of bacteria in gastric mucosa of all samples; (b) histogram of the relative abundance of bacteria in gastric mucosa of two groups; and (c) the TOP15 genus level abundance cluster diagram of all samples.

Figure 6 The expression of the TOP5 genera in the samples of both two groups detected via PCR analysis. (a) L. multiparus; (b) H. pylori; (c) B. fragilis; (d) K. peneumoniae; and (e) P. aeruginosa.
Figure 6

The expression of the TOP5 genera in the samples of both two groups detected via PCR analysis. (a) L. multiparus; (b) H. pylori; (c) B. fragilis; (d) K. peneumoniae; and (e) P. aeruginosa.

The relative abundance of other taxonomic levels (class, order, family, and species) was depicted in histograms and heatmap in Figure S3.

3.6 Differences in microbiome compositions between the groups

Multivariate statistical analysis of microbial variables was employed to identify species with significant differences between the two groups. As depicted in Table 4, at the T-test level, there were 108 differential OTUs, 1 differential phylum, and 23 differential genera between the two groups. At the Wilcoxon rank-sum test level, the number of differential OTUs, differential phylum, and differential genera was 322, 2, and 46, respectively. Furthermore, the linear discriminant analysis effect size analysis was utilized to identify the potential differences in the abundance of the various bacterial taxa between the two groups (all had LDA scores >3.0 and P <  0.05, Figure S4).

Table 4

The number of species differences between the HP_Pos group and the HP_Neg group at different taxonomic levels

Method Out Phylum Class Order Family Genus Species
T_test 108 1 4 4 8 23 15
Wilcoxon 322 2 5 11 14 46 31

Significant differences in the genera of the TOP10 species with diverse abundance were observed between the groups, as analyzed by the Wilcoxon rank-sum test (P < 0.05, Table 5, Figure 7a). Specifically, the abundances of Helicobacter, Christensenellaceae_R-7_group, Desulfovibrio, Cetobacterium, Prevotella, Brevibacillus, Promicromonospor, Pseudoxanthomonas, Dialister, and Micromonospora differed significantly between the HP_Pos group and the HP_Neg group. Furthermore, the results of the heatmap analysis based on the relative abundance of species differing at the genus level revealed a discriminatory abundance at the genus level between the two groups (Figure 7b). The HP_Pos group exhibited higher abundances in Geobacter, Esulfitobacterium, Desulfovibrio, Curvibacter, Anaerocella, Uncultured delta proteobacteriurn, Sva0081_sedirment_group, Thauera, Ralstonia, Brevibacteriurn, Thermomonospora, Uncultured_Acidobacteriales_bacterium, Pannonibacter, Mucilaginibacter, Acidipila, Treponema, Cetobacterium, Salininicrobium, Helicobacter, Virgisporangium, Plot4-2H12, Ruminococcaceae_ucG-a11, Mle1-7, Leucobacter, Petrimonas, Sodimentibacter, Christensenellaceae_R-7_group, Agromyces, Ensifer, and Filobacterium. In contrast, genera such as Mathylovarus, Actinophyrtocola, Rhodoplanes, Pseudoxanthononas, Pranicromonospora, Brevibecillus, Uncultured_bacterium_0319_6E22, Photabacterium, Dialister, Anaerovorax, and Micromonospora displayed higher abundances in the HP_Neg group.

Table 5

Wilcoxon statistical difference species

Taxon HP_Pos group HP_Neg group P value
Helicobacter 0.04961 0.02754 0.02
Christensenellaceae_R-7_group 0.00305 0.00183 0.03
Desulfovibrio 0.00216 0.00123 0.03
Cetobacterium 0.00113 0.00031 0.01
Prevotella 0.00030 0.00060 0.04
Brevibacillus 0.00024 0.00065 0.02
Promicromonospora 0.00017 0.00045 0.01
Pseudoxanthomonas 0.00018 0.00043 0.02
Dialister 0.00019 0.00036 0.04
Micromonospora 0.00013 0.00031 0.03
Figure 7 Multivariate statistical analysis of microbial variables. (a) TOP10 boxplot and (b) TOP10 heatmap of different species abundance (at genus level) in gastric mucosa of two groups. (c) Important species at the genus level were analyzed by random Forest analysis.
Figure 7

Multivariate statistical analysis of microbial variables. (a) TOP10 boxplot and (b) TOP10 heatmap of different species abundance (at genus level) in gastric mucosa of two groups. (c) Important species at the genus level were analyzed by random Forest analysis.

Utilizing random forest analysis to identify the key components (OTUs or species) capable of distinguishing differences between two groups of samples is an effective approach for classification and prediction. In this study, an analysis of the TOP30 genera in terms of relative abundance, coupled with the plotting of species importance points using a random forest model, revealed Helicobacter as the representative genus capable of stably distinguishing between the two groups of samples, indicating that it was a crucial measure (Figure 7c).

3.7 PICRUSt functional prediction analysis

Based on PICRUSt for predicting the metabolic pathways, the functional gene enrichment in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways at three different levels (KEGG_L1, KEGG_L2, and KEGG_L3) was obtained. The observed high expression of H. pylori at these three levels in the two groups of samples suggested that H. pylori might exert different weights on different functions (Figures S5–S7). According to the Wilcoxon test, there was no significant difference found between the two groups in the main pathway at the KEGG_L1 level; in the metabolic pathways at the KEGG_L2 level, only the circulatory system exhibited a significant difference between the two groups (P = 0.03); meanwhile, in the metabolic pathways at the KEGG_L3 level, the HP_Pos group and the HP_Neg group exhibited significant differences in seven functions, namely endocrine and other factor-regulated calcium reabsorption (P = 0.004), salivary secretion (P = 0.004), gastric acid secretion (P = 0.004), aldosterone-regulated sodium reabsorption (P = 0.014), amoebiasis (P = 0.027), Parkinson’s disease (P = 0.021), and cardiac muscle contraction (P = 0.027). According to the functional difference results of the Wilcoxon test, a heatmap diagram was drawn as depicted in Figure 8. The results revealed that five samples in the HP_Pos group exhibited higher expression in the functions of aldosterone-regulated sodium reabsorption, gastric acid secretion, endocrine and other factor-regulated calcium reabsorption, and salivary secretion. Additionally, the remaining samples in the HP_Pos group also demonstrated pronounced expressed in amoebiasis, Parkinson’s disease, and cardiac muscle contraction compared to those in the HP_Neg group.

Figure 8 Heatmap graph of clustering of KEGG difference results.
Figure 8

Heatmap graph of clustering of KEGG difference results.

3.8 The pathogenic mechanisms of H. pylori infection in vivo model

To gain a deeper comprehension of the impact of H. pylori infection on gastric acid secretion and the underlying pathogenic mechanisms of this bacterium, a mice model of H. pylori infection was established. The experimental cohort was designated as the HP_Pos group, wherein mice were subjected to H. pylori infection, while the HP_Neg group, serving as the control, received gastric lavage with an equivalent volume of physiological saline. Gastric acid secretion displayed a remarkable increase over the course of prolonged H. pylori infection (P < 0.01). Notably, in comparison to the HP_Neg group, the mice in the HP_Pos group consistently demonstrated significantly elevated levels of gastric acid secretion, observed distinctly at both 6 and 12 weeks (P < 0.001, Figure 9a). In addition, the recorded gastritis scores for activity were significantly increased in the HP_Pos group mice in comparison to those in the HP_Neg group at both the 6-week (P < 0.01) and 12-week (P < 0.001) intervals following inoculation (Figure 9b). The findings from H&E staining revealed pronounced infiltration of neutrophils and lymphocytes in mice infected with H. pylori, noticeable at both the 6- and 12-week intervals following inoculation. In contrast, minimal or absent occurrences of neutrophil and lymphocyte infiltrations were discernible in the HP_Neg groups (Figure 9c). Moreover, the determination of the MDA concentration through ELISA analysis revealed a congruence in trends with that of gastric acid secretion (Figure 9d).

Figure 9 In vivo validation experiments for KEGG pathway. Alterations in (a) gastric acid secretion and (b) gastritis scores for activity observed at 6 and 12 weeks after inoculation in mice. (c) Histopathological changes in the gastric mucosa of mice detected by H&E staining. (d) Quantification of gastric MDA concentration determined via ELISA.
Figure 9

In vivo validation experiments for KEGG pathway. Alterations in (a) gastric acid secretion and (b) gastritis scores for activity observed at 6 and 12 weeks after inoculation in mice. (c) Histopathological changes in the gastric mucosa of mice detected by H&E staining. (d) Quantification of gastric MDA concentration determined via ELISA.

4 Discussion

With the continuous advancement of high-throughput sequencing and other detection technologies, numerous investigations both domestically and internationally have illuminated the existence of a sophisticated microbial architecture and a distinctive microecological milieu in the human stomach. Nonetheless, the structure and functionality of the gastric flora, as well as its intricate relationship with human health and diseases, remain yet to be definitively elucidated. Consequently, in this study, the gastric mucosal tissues from H. pylori-positive and H. pylori-negative CG patients were collected, and the structural characteristics and differences in the gastric mucosal microbiota of these two patient cohorts were analyzed employing 16S rRNA high-throughput sequencing technology and PCR analysis. In addition, valuable insights into the impact of H. pylori infection on gastric acid secretion, along with its underlying pathogenic mechanisms, were gleaned through animal experiments. Our study has laid a robust foundation for examining the association between the composition of gastric microflora and the onset and progression of CG.

In the 16S gene variable regions, V3–V4 manifest the most extensive taxonomic coverage, diversity, reproducibility, and PCR amplification efficiency [18], rendering them the optimal choice for Illumina high-throughput sequencing. As per the outcomes emerging from the α-diversity analysis of OTUs, no notable dissimilarity was observed in the abundance and variety of gastric mucosal microbiota between H. pylori-positive and H. pylori-negative CG patients, indicating that the sampling in this study was adequate, with the sequencing volume achieving saturation sufficient to encompass the vast majority of species, the richness and homogeneity of the flora were high, and the samples obtained were ample for the subsequent analysis. In addition, the outcomes of the β-diversity analysis indicated a resemblance in the bacterial community structure of the gastric mucosa between the two aforementioned groups. Animal experimentation has demonstrated that the abundance and the community composition of the gastric mucosal flora in C57BL/6 mice, whether with acute or chronic H. pylori infection, experienced no noteworthy alterations [19]. These observations align with findings from human investigations. Both Khosravi et al. [20] and Coker et al. [21] discerned no noteworthy correlation between the composition of the human gastric flora and H. pylori infection, thereby lending support to the results obtained in our study.

In this investigation, it was discerned that the dominant bacteria in the stomach of CG patients, both with and without H. pylori infection, encompassed Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Epsilonbacteraeota, but the proportion of these bacteria in the two groups was different. A study conducted by Cho et al. [22] similarly illustrated that the stomach microbes of both H. pylori-negative and H. pylori-positive individuals contained the same phylum, albeit in varying percentages of abundance. Although no significant disparity was observed in the α and β diversity of the gastric mucosal microflora between the two groups, the Wilcoxon statistical analysis unveiled noteworthy distinctions in the bacterial colonies at the levels of phylum, class, order, family, genus, and species, concurrently signifying that H. pylori infection may instigate disruption and disarray in the gastric microflora. Upon the colonization of H. pylori, an attenuation in the protective efficacy of the gastric mucosa ensued, giving rise to a sequence of pathological metamorphoses. This cascade culminated in the induction of disorder and dysbiosis in the gastric flora, accompanied by alterations in the gastric microenvironment [23]. Furthermore, the HP_Pos group exhibited markedly elevated proportions of Epsilonbacteraeota and Fusobacteria, distinctly surpassing their counterparts in the HP_Neg group. Epsilonbacteraeota, identified as one of the most prevalent phylum in patients with H. pylori infection, and the uniqueness of the flagellum of Epsilonbacteraeota may be one of the potential reasons for its significant divergence from other flora, which may be related to the mechanism of gastric mucosa inflammation in CG patients and could entail distinct metabolic functional pathways from the pathogenic H. pylori [24]. Fusobacteria, a gram-negative bacterium commonly found in the gastrointestinal tract, possesses invasive, adhesive, and inflammatory effects, playing a pivotal role in the development of colorectal cancer and its precancerous lesions by secreting substances that activate oncogenes and participate in the pro-carcinogenic pathway [25]. Hence, it is speculated that H. pylori infection might augment the invasion and metabolic capacity of Epsilonbacteraeota and Fusobacteria, which alter the inflammatory state of the gastric mucosa, and perhaps even accelerate the risk of chronic inflammation to tumorigenic transformation, the interrelationship among these three necessitate further comprehensive investigation.

Based on PICRUSt for predicting the metabolic pathways, the functional gene enrichment in KEGG pathways at three different levels was obtained. Upon scrutinizing the differential metabolic pathways and functional variations observed at the KEGG_L3 level heatmap between the two groups of samples, it is plausible to infer that H. pylori infection may exert a certain influence on endocrine function. Recent studies have compellingly suggested a modulator role for H. pylori in the endocrine system [26]. This participates in the secretion or dysregulation of specific functions, indicating a potential correlation with disorders associated with secretion dysregulation. Of particular interest is the elevated expression observed in the gastric acid secretion functionality in the HP_Pos group, evoking our keen interest in exploring this phenomenon further. Subsequently, we proceeded to establish a murine model of H. pylori infection, which yielded findings congruent with the sequencing results. Notably, a positive correlation surfaced between gastric acid secretion and the gastritis scores for activity; in other words, as the severity of gastritis increased, there was a corresponding augmentation in gastric acid secretion. In addition, histological analysis unveiled pronounced infiltration of neutrophils and lymphocytes in the mice infected with H. pylori. Prior investigations have elucidated that the pathogenic impact of H. pylori encompasses gastrointestinal tract colonization and substantial generation of reactive oxygen species (ROS) by the neutrophils congregating at the infection site [27,28]. Consequently, we conducted ELISA experiments to measure gastric MDA concentration, revealing a conspicuous elevation. This underscores the tendency of H. pylori to incite robust ROS production through the perpetual activation of neutrophils, thus accentuating gastric inflammation and amplifying gastric acid secretion, aligning with the existing literature reports.

In conclusion, this study revealed that while no significant differences were detected in the abundance, diversity, and structure of the bacterial flora in the gastric mucosa samples of CG patients between the two groups, there was a notable disparity in bacterial flora between the two groups at various classification levels, suggesting that H. pylori infection would instigate the disturbance of the gastric flora and imbalance of gastric microecological stability, leading to gastric dysfunction and a cascade of pathological processes through the related influence of other bacterial flora. Furthermore, H. pylori infection activates neutrophils continually attracted to the infected gastric mucosa, triggering substantial production of ROS. As a result, the inflammatory damage to the gastric mucosa becomes challenging to repair, and the infection remains chronic and inflammatory over an extended period. The limitations of this study primarily lie in the insufficient classification and grading of CG caused by H. pylori infection during endoscopy, and the number of cases included in this study remains relatively small. To comprehensively elucidate the interactions between H. pylori and other microbial communities within the stomach, it is imperative to augment the sample size in future investigations. In addition, the sequencing samples were exclusively obtained from the gastric body and the gastric sinus in this investigation. Nonetheless, as the gastric body and gastric sinus serve as primary sites of gastritis, they possess a certain capacity to reflect the overall microbial landscape within the stomach. Lastly, for the pathogenic flora that is co-involved in pathogenesis with H. pylori, future therapeutic approaches could focus on inhibiting its metabolic function to block its pathogenic pathway and diminish its value and pathogenic function.

tel: +86 15240758591

  1. Funding information: This study was supported by the National Natural Science Foundation of China (31460023) and City-school Cooperation Project of Baise Science Research and Technology Development Plan (2017081).

  2. Author contributions: C Cen: project development, data analysis, and manuscript writing; QY Du: project development, data analysis and collection, and manuscript writing; B Luo: project development, data analysis, and manuscript writing; TH Wang: project development, data analysis and collection, and manuscript editing; JW Su: project development, data analysis, and manuscript writing; XS Qin: project development, data analysis and collection, and manuscript writing; WY Zhang: project development, data analysis, and manuscript writing; LJ Lu: project development, data analysis and collection, and manuscript editing; Y Liao: project development, data analysis, and manuscript writing; YQ Huang: Animal experiment operation; YM Liang: project development, data analysis, and manuscript writing/editing.

  3. Conflict of interest: Authors state no conflict of interest.

  4. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request. Data analyzed are available in the Sequence Read Archive repository, https://www.ncbi.nlm.nih.gov/sra/PRJNA929676.

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Received: 2023-09-19
Revised: 2024-01-25
Accepted: 2024-02-06
Published Online: 2024-03-28

© 2024 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  136. Differential expression of phosphoinositide 3-kinase/protein kinase B and Toll-like receptor/nuclear factor kappa B signaling pathways in experimental obesity Wistar rat model
  137. The therapeutic potential of targeting Oncostatin M and the interleukin-6 family in retinal diseases: A comprehensive review
  138. BA inhibits LPS-stimulated inflammatory response and apoptosis in human middle ear epithelial cells by regulating the Nf-Kb/Iκbα axis
  139. Role of circRMRP and circRPL27 in chronic obstructive pulmonary disease
  140. Investigating the role of hyperexpressed HCN1 in inducing myocardial infarction through activation of the NF-κB signaling pathway
  141. Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies
  142. Quantitative immunohistochemistry analysis of breast Ki67 based on artificial intelligence
  143. Ecology and Environmental Science
  144. Screening of different growth conditions of Bacillus subtilis isolated from membrane-less microbial fuel cell toward antimicrobial activity profiling
  145. Degradation of a mixture of 13 polycyclic aromatic hydrocarbons by commercial effective microorganisms
  146. Evaluation of the impact of two citrus plants on the variation of Panonychus citri (Acari: Tetranychidae) and beneficial phytoseiid mites
  147. Prediction of present and future distribution areas of Juniperus drupacea Labill and determination of ethnobotany properties in Antalya Province, Türkiye
  148. Population genetics of Todarodes pacificus (Cephalopoda: Ommastrephidae) in the northwest Pacific Ocean via GBS sequencing
  149. A comparative analysis of dendrometric, macromorphological, and micromorphological characteristics of Pistacia atlantica subsp. atlantica and Pistacia terebinthus in the middle Atlas region of Morocco
  150. Macrofungal sporocarp community in the lichen Scots pine forests
  151. Assessing the proximate compositions of indigenous forage species in Yemen’s pastoral rangelands
  152. Food Science
  153. Gut microbiota changes associated with low-carbohydrate diet intervention for obesity
  154. Reexamination of Aspergillus cristatus phylogeny in dark tea: Characteristics of the mitochondrial genome
  155. Differences in the flavonoid composition of the leaves, fruits, and branches of mulberry are distinguished based on a plant metabolomics approach
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  157. Non-linear associations between cardiovascular metabolic indices and metabolic-associated fatty liver disease: A cross-sectional study in the US population (2017–2020)
  158. Knockdown of USP7 alleviates atherosclerosis in ApoE-deficient mice by regulating EZH2 expression
  159. Utility of dairy microbiome as a tool for authentication and traceability
  160. Agriculture
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  162. Impact of novel herbicide based on synthetic auxins and ALS inhibitor on weed control
  163. Perspectives of pteridophytes microbiome for bioremediation in agricultural applications
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  165. Improving the productivity and profitability of maize (Zea mays L.) using optimum blended inorganic fertilization
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  169. Development of a detection chip for major pathogenic drug-resistant genes and drug targets in bovine respiratory system diseases
  170. Exploration of the genetic influence of MYOT and MB genes on the plumage coloration of Muscovy ducks
  171. Transcriptome analysis of adipose tissue in grazing cattle: Identifying key regulators of fat metabolism
  172. Comparison of nutritional value of the wild and cultivated spiny loaches at three growth stages
  173. Transcriptomic analysis of liver immune response in Chinese spiny frog (Quasipaa spinosa) infected with Proteus mirabilis
  174. Disruption of BCAA degradation is a critical characteristic of diabetic cardiomyopathy revealed by integrated transcriptome and metabolome analysis
  175. Plant Sciences
  176. Effect of long-term in-row branch covering on soil microorganisms in pear orchards
  177. Photosynthetic physiological characteristics, growth performance, and element concentrations reveal the calcicole–calcifuge behaviors of three Camellia species
  178. Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation
  179. Bioinformatics, expression analysis, and functional verification of allene oxide synthase gene HvnAOS1 and HvnAOS2 in qingke
  180. Water, nitrogen, and phosphorus coupling improves gray jujube fruit quality and yield
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  182. Role of Embinin in the reabsorption of nucleus pulposus in lumbar disc herniation: Promotion of nucleus pulposus neovascularization and apoptosis of nucleus pulposus cells
  183. Revealing the effects of amino acid, organic acid, and phytohormones on the germination of tomato seeds under salinity stress
  184. Combined effects of nitrogen fertilizer and biochar on the growth, yield, and quality of pepper
  185. Comprehensive phytochemical and toxicological analysis of Chenopodium ambrosioides (L.) fractions
  186. Impact of “3414” fertilization on the yield and quality of greenhouse tomatoes
  187. Exploring the coupling mode of water and fertilizer for improving growth, fruit quality, and yield of the pear in the arid region
  188. Metagenomic analysis of endophytic bacteria in seed potato (Solanum tuberosum)
  189. Antibacterial, antifungal, and phytochemical properties of Salsola kali ethanolic extract
  190. Exploring the hepatoprotective properties of citronellol: In vitro and in silico studies on ethanol-induced damage in HepG2 cells
  191. Enhanced osmotic dehydration of watermelon rind using honey–sucrose solutions: A study on pre-treatment efficacy and mass transfer kinetics
  192. Effects of exogenous 2,4-epibrassinolide on photosynthetic traits of 53 cowpea varieties under NaCl stress
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  194. An optimization method for measuring the stomata in cassava (Manihot esculenta Crantz) under multiple abiotic stresses
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  196. Antioxidant and antimicrobial activities of Salsola imbricata methanolic extract and its phytochemical characterization
  197. Bioengineering and Biotechnology
  198. Absorbable calcium and phosphorus bioactive membranes promote bone marrow mesenchymal stem cells osteogenic differentiation for bone regeneration
  199. New advances in protein engineering for industrial applications: Key takeaways
  200. An overview of the production and use of Bacillus thuringiensis toxin
  201. Research progress of nanoparticles in diagnosis and treatment of hepatocellular carcinoma
  202. Bioelectrochemical biosensors for water quality assessment and wastewater monitoring
  203. PEI/MMNs@LNA-542 nanoparticles alleviate ICU-acquired weakness through targeted autophagy inhibition and mitochondrial protection
  204. Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells
  205. Erratum
  206. Erratum to “Investigating the association between dietary patterns and glycemic control among children and adolescents with T1DM”
  207. Erratum to “Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation”
  208. Retraction
  209. Retraction to “MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB”
  210. Retraction to “A data mining technique for detecting malignant mesothelioma cancer using multiple regression analysis”
  211. Special Issue on Advances in Neurodegenerative Disease Research and Treatment
  212. Transplantation of human neural stem cell prevents symptomatic motor behavior disability in a rat model of Parkinson’s disease
  213. Special Issue on Multi-omics
  214. Inflammasome complex genes with clinical relevance suggest potential as therapeutic targets for anti-tumor drugs in clear cell renal cell carcinoma
  215. Gastroesophageal varices in primary biliary cholangitis with anti-centromere antibody positivity: Early onset?
https://doi.org/10.1515/biol-2022-0839
Keywords for this article
chronic gastritis; Helicobacter pylori; 16S ribosomal RNA; operational taxonomic unit; Kyoto encyclopedia of genes and genomes
Creative Commons
BY 4.0

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  148. Population genetics of Todarodes pacificus (Cephalopoda: Ommastrephidae) in the northwest Pacific Ocean via GBS sequencing
  149. A comparative analysis of dendrometric, macromorphological, and micromorphological characteristics of Pistacia atlantica subsp. atlantica and Pistacia terebinthus in the middle Atlas region of Morocco
  150. Macrofungal sporocarp community in the lichen Scots pine forests
  151. Assessing the proximate compositions of indigenous forage species in Yemen’s pastoral rangelands
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  153. Gut microbiota changes associated with low-carbohydrate diet intervention for obesity
  154. Reexamination of Aspergillus cristatus phylogeny in dark tea: Characteristics of the mitochondrial genome
  155. Differences in the flavonoid composition of the leaves, fruits, and branches of mulberry are distinguished based on a plant metabolomics approach
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  157. Non-linear associations between cardiovascular metabolic indices and metabolic-associated fatty liver disease: A cross-sectional study in the US population (2017–2020)
  158. Knockdown of USP7 alleviates atherosclerosis in ApoE-deficient mice by regulating EZH2 expression
  159. Utility of dairy microbiome as a tool for authentication and traceability
  160. Agriculture
  161. Enhancing faba bean (Vicia faba L.) productivity through establishing the area-specific fertilizer rate recommendation in southwest Ethiopia
  162. Impact of novel herbicide based on synthetic auxins and ALS inhibitor on weed control
  163. Perspectives of pteridophytes microbiome for bioremediation in agricultural applications
  164. Fertilizer application parameters for drip-irrigated peanut based on the fertilizer effect function established from a “3414” field trial
  165. Improving the productivity and profitability of maize (Zea mays L.) using optimum blended inorganic fertilization
  166. Application of leaf multispectral analyzer in comparison to hyperspectral device to assess the diversity of spectral reflectance indices in wheat genotypes
  167. Animal Sciences
  168. Knockdown of ANP32E inhibits colorectal cancer cell growth and glycolysis by regulating the AKT/mTOR pathway
  169. Development of a detection chip for major pathogenic drug-resistant genes and drug targets in bovine respiratory system diseases
  170. Exploration of the genetic influence of MYOT and MB genes on the plumage coloration of Muscovy ducks
  171. Transcriptome analysis of adipose tissue in grazing cattle: Identifying key regulators of fat metabolism
  172. Comparison of nutritional value of the wild and cultivated spiny loaches at three growth stages
  173. Transcriptomic analysis of liver immune response in Chinese spiny frog (Quasipaa spinosa) infected with Proteus mirabilis
  174. Disruption of BCAA degradation is a critical characteristic of diabetic cardiomyopathy revealed by integrated transcriptome and metabolome analysis
  175. Plant Sciences
  176. Effect of long-term in-row branch covering on soil microorganisms in pear orchards
  177. Photosynthetic physiological characteristics, growth performance, and element concentrations reveal the calcicole–calcifuge behaviors of three Camellia species
  178. Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation
  179. Bioinformatics, expression analysis, and functional verification of allene oxide synthase gene HvnAOS1 and HvnAOS2 in qingke
  180. Water, nitrogen, and phosphorus coupling improves gray jujube fruit quality and yield
  181. Improving grape fruit quality through soil conditioner: Insights from RNA-seq analysis of Cabernet Sauvignon roots
  182. Role of Embinin in the reabsorption of nucleus pulposus in lumbar disc herniation: Promotion of nucleus pulposus neovascularization and apoptosis of nucleus pulposus cells
  183. Revealing the effects of amino acid, organic acid, and phytohormones on the germination of tomato seeds under salinity stress
  184. Combined effects of nitrogen fertilizer and biochar on the growth, yield, and quality of pepper
  185. Comprehensive phytochemical and toxicological analysis of Chenopodium ambrosioides (L.) fractions
  186. Impact of “3414” fertilization on the yield and quality of greenhouse tomatoes
  187. Exploring the coupling mode of water and fertilizer for improving growth, fruit quality, and yield of the pear in the arid region
  188. Metagenomic analysis of endophytic bacteria in seed potato (Solanum tuberosum)
  189. Antibacterial, antifungal, and phytochemical properties of Salsola kali ethanolic extract
  190. Exploring the hepatoprotective properties of citronellol: In vitro and in silico studies on ethanol-induced damage in HepG2 cells
  191. Enhanced osmotic dehydration of watermelon rind using honey–sucrose solutions: A study on pre-treatment efficacy and mass transfer kinetics
  192. Effects of exogenous 2,4-epibrassinolide on photosynthetic traits of 53 cowpea varieties under NaCl stress
  193. Comparative transcriptome analysis of maize (Zea mays L.) seedlings in response to copper stress
  194. An optimization method for measuring the stomata in cassava (Manihot esculenta Crantz) under multiple abiotic stresses
  195. Fosinopril inhibits Ang II-induced VSMC proliferation, phenotype transformation, migration, and oxidative stress through the TGF-β1/Smad signaling pathway
  196. Antioxidant and antimicrobial activities of Salsola imbricata methanolic extract and its phytochemical characterization
  197. Bioengineering and Biotechnology
  198. Absorbable calcium and phosphorus bioactive membranes promote bone marrow mesenchymal stem cells osteogenic differentiation for bone regeneration
  199. New advances in protein engineering for industrial applications: Key takeaways
  200. An overview of the production and use of Bacillus thuringiensis toxin
  201. Research progress of nanoparticles in diagnosis and treatment of hepatocellular carcinoma
  202. Bioelectrochemical biosensors for water quality assessment and wastewater monitoring
  203. PEI/MMNs@LNA-542 nanoparticles alleviate ICU-acquired weakness through targeted autophagy inhibition and mitochondrial protection
  204. Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells
  205. Erratum
  206. Erratum to “Investigating the association between dietary patterns and glycemic control among children and adolescents with T1DM”
  207. Erratum to “Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation”
  208. Retraction
  209. Retraction to “MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB”
  210. Retraction to “A data mining technique for detecting malignant mesothelioma cancer using multiple regression analysis”
  211. Special Issue on Advances in Neurodegenerative Disease Research and Treatment
  212. Transplantation of human neural stem cell prevents symptomatic motor behavior disability in a rat model of Parkinson’s disease
  213. Special Issue on Multi-omics
  214. Inflammasome complex genes with clinical relevance suggest potential as therapeutic targets for anti-tumor drugs in clear cell renal cell carcinoma
  215. Gastroesophageal varices in primary biliary cholangitis with anti-centromere antibody positivity: Early onset?
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