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Computational and experimental investigation of antibacterial and antifungal properties of Nicotiana tabacum extracts

  • Muhammad Hamayoon Khan , Abdur Rauf EMAIL logo , Muhammad Saeed , Taghrid S. Alomar EMAIL logo , Anees Ahmed Khalil , Najla AlMasoud , Rohit Sharma and Giovanni Ribaudo EMAIL logo
Published/Copyright: June 20, 2023
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Open Chemistry
From the journal Open Chemistry Volume 21 Issue 1

Abstract

The identification of novel anti-infective agents of synthetic and natural origin represents one of the main aims of contemporary drug discovery. In the current work, four different varieties of Nicotiana tabacum, namely, K399, SPG28, Swat No. 1, and Swat No. 2, were studied to assess the antibacterial and antifungal properties of their extracts. The extracts contain anthraquinones, alkaloids, saponins, terpenoids, tannins, resins, steroids, proteins, and carbohydrates, and the antibacterial and antifungal activities were evaluated toward four bacterial and four fungal strains. N. tabacum K399 showed the highest zone of inhibition against E. coli. Similarly, K399 showed the highest antifungal potential, as the highest zone of inhibition for the set was detected against C. albicans. Then, the underlying molecular mechanism was further investigated, and the extracts were tested for their inhibitory potential against urease, an enzyme which is conserved in bacteria and fungi. Additionally, computational tools were enrolled to assess the role of rutin and chlorogenic acid, which are among the main constituents of N. tabacum leaves, in interacting with urease through molecular docking. Combined together, the computational and experimental results support the antibacterial and antifungal potential of N. tabacum extracts, particularly, that obtained from K399 variety.

Keywords: Nicotiana tabacum ; rutin; chlorogenic acid; antibacterial; antifungal

1 Introduction

The term “tobacco” refers to the processed leaves of diverse plant species from the genus Nicotiana belonging to the family of Solanaceae, which are abundantly cultivated in Asia, America, Australia, and southwestern Africa [1–3]. Several varieties of Nicotiana species are not only consumed primarily for recreational purposes but also for medicinal purposes [4]. More specifically, even though nearly 60–75 different species have been identified, N. tabacum L. and N. rustical L. are predominantly grown for human use [5].

Several studies aiming at determining the phytochemical constituents of Nicotiana species and their associated bioactivities were reported [6,7] as N. tabacum, in particular, is found to be a rich source of various bioactive compounds with antimicrobial, anti-inflammatory, and antioxidant potential. In this connection, N. tabacum contains several active constituents besides the eponymous nicotine, including flavonoids, phenolic acids, alkaloids, terpenoids, etc., which are responsible for its different biological effects [6,8–10]. Besides, the phytochemical profile of N. tabacum leaves is widely affected by various factors like dehydration, treatment process, fermentation, and storage. Polyphenols account for nearly 7% of the dry weight of tobacco and their content varies depending upon variety, stage of harvesting, maturation stage, and other environmental parameters such as temperature [11]. More specifically, the polyphenolic composition of tobacco includes rutin, chlorogenic acid, flavone, and scopolamine. Interestingly, among these species, rutin and chlorogenic acid account for nearly 80% of the total polyphenol content of N. tabacum leaves [12–14].

In contemporary drug discovery, great attention and efforts are pointed towards the world of natural products for the identification of novel antibacterial [15] and antifungal agents [16,17]. More specifically, recent contributions in the literature shed light on the potential of N. tabacum extracts to contrast the proliferation of bacteria and fungi [18,19]. In this context, the aim of the current study is to evaluate the potential of extracts from K399, SPG38, Swat No. 1 (SN1) and Swat No. 2 (SN2) varieties of N. tabacum as antifungal and antibacterial agents. The extracts were tested in vitro, and urease inhibition was also evaluated. Additionally, computational studies were carried out to support the interpretation of experimental data.

2 Methods

2.1 Samples collection and extraction

Four different indigenously available varieties of N. tabacum, i.e., SN1, SN2, SPG28, and K399, were collected from local farmers of Swabi District, Khyber Pakhtunkhwa (KPK), Pakistan. These samples were brought to the Department of Chemistry, University of Swabi, KPK, Pakistan for further processing, and the sample voucher NO.UOS-BOT/120 was deposited in the Department of Botany, University of Swabi.

Leaves from the four different species of N. tabacum were separated from stems, cleaned, washed, and dried for 2 weeks in the shade. After drying, plant material (2 kg) was grinded, and the resulting powder was collected and used for further analysis [20]. Then, powdered leaves (100 g) from the four different varieties were placed in a 1 L volumetric flask with 800 mL of methanol for 7 days. Afterwards, the mixture was filtered using grade 1 filter paper from Whatman (Maidstone, UK), and the filtrate was evaporated under reduced pressure to provide the crude extracts, which were stored at −20°C. All used chemicals, reagents, and solvents were of analytical grade and were obtained from Honeywell-Fluka (Charlotte, NC, USA), Sigma-Aldrich (St. Louis, MO, USA), and Merck (Darmstadt, Germany).

2.2 Preliminary phytochemical analysis

The preliminary screening for the detection of different phytochemical compounds like saponins, proteins, steroids, flavonoids, alkaloids, tannins, anthraquinones, terpenoids, and carbohydrates was performed using standard protocols based on qualitative assays [21–23]. All experiments were carried out in triplicate.

2.3 In vitro antibacterial and antifungal activity

Well diffusion method was adopted to evaluate the antibacterial potential of extracts prepared from four varieties of N. tabacum against S. typhi, E. coli, B. subtilis, and S. aureus [24]. Ampicillin was used as reference compound in this study, and the zone of inhibition (mm) was measured to assess the antibacterial properties. All the tests were performed in triplicate and mean value ± SD was calculated.

The broth dilution method was employed for the determination of antifungal properties of extracts prepared from four varieties of N. tabacum against T. harzianum, A. brasiliensis, C. albicans, and A. niger [24]. Mycostatin was used as reference compound in this study. The inoculum was added to each tube and incubated for 7 days. Following incubation, the antifungal properties of tubes containing standard drug and extracts were evaluated. The zone of inhibition (mm) was then calculated to assess the antifungal properties. All the tests were performed in triplicate and mean value ± SD was calculated.

2.4 Urease inhibition

Jack bean urease inhibitory activity of the extracts was determined by adopting previously reported procedures [24,25]. Briefly, each extract (25 µL) and enzyme (25 µL of 0.25 mg/mL solution) were incubated for 15 min at 30°C. After the initial incubation, urea (55 µL) was added to the mixture, which was again incubated in the same conditions. Then, 45 µL of phenol reagent was introduced in each well (0.005% w/v sodium nitroprusside and 1% w/v phenol) together with 70 µL of alkali reagent (0.1% w/v NaClO and 0.5% w/v NaOH). The absorbance (A) of this mixture was measured at 630 nm. The final mixtures were incubated for additional 50 min at 30°C, and the absorbance at 630 nm was then measured. Thiourea was used as a positive control in this analysis. Percent inhibition of urease was calculated by using the following formula:

Percent inhibition = ( A blank − A sample ) A blank × 100

All the tests were performed in triplicate and the mean value ± SD was calculated.

2.5 Molecular modeling

The structure of the macromolecular target was retrieved from the RCSB Protein Data Bank (www.rcsb.org, PDB ID 3LA4) and the PDB file was selected in agreement with previous works [26,27]. 3D models of ligands were built using Avogadro 1.2.0, and geometry was optimized using the same software [28]. Target was prepared for the blind docking experiments, performed using AutoDock Vina, using default parameters [29]. Receptor search volume was set according to the grid parameters reported as follows: Grid center: x = −52.260, y = −13.702, z = 77.095, size: 75 × 95 × 80 Å. The number of docking poses was set to 10, with other Vina parameters set as default. Residue numbering used in the PDB file was adopted. Output data, such as calculated binding energies and interaction patterns, were analyzed and scored using UCSF Chimera molecular viewer [30]. This software was also used to produce the artworks. Calculated binding energy values are expressed in kcal/mol and refer to the most favored predicted pose.

3 Results

The aim of this study is to evaluate the potential of the extracts of different varieties of N. tabacum, namely, K399, SPG28, SN1, and SN2 (Figure 1) as antibacterial and antifungal agents. The first step consists of the extraction of bioactive components. In particular, after processing, the methanolic extracts obtained from dried leaves of the four samples were subjected to qualitative assays to preliminarily determine their content in terms of classes of phytochemical constituents.

Figure 1 Images of the plant samples collected and studied in the current work. K399, SPG28, SN1 and SN2 varieties are depicted clockwise starting from top left.
Figure 1

Images of the plant samples collected and studied in the current work. K399, SPG28, SN1 and SN2 varieties are depicted clockwise starting from top left.

The qualitative phytochemical analysis was performed to assess the presence or absence of specific chemical classes of bioactive secondary metabolites in the different varieties [23], and the results are reported in Table S1 in the Supporting information.

More specifically, the K399 methanolic extract showed the presence of tannins, alkaloids, saponins, flavonoids, steroids, terpenoids, carbohydrates, polyphenols, and polypeptides, while anthraquinones, C-glycosides, and resins were not present. On the other hand, anthraquinones, alkaloids, saponins, terpenoids, flavonoids, steroids, carbohydrates, C-glycosides, and polyphenols were detected in the extract from the SPG38 variety, while tannins, resins, and polypeptides were not present. The SN1 methanolic extract showed the presence of tannins, alkaloids, steroids, saponins, flavonoids, resins, carbohydrates, and polypeptides, while C-glycosides and terpenoids were absent. Eventually, the SN2 methanolic extract contained anthraquinones, alkaloids, steroids, flavonoids, resins, carbohydrates, cardiac glycosides, polyphenols, and polypeptides, while tannins, saponins, steroids, terpenoids, and polypeptides were not present.

Overall, phytochemical screening of extracts from all four different varieties of N. tabacum revealed the presence of alkaloids, flavonoids, polyphenols, and carbohydrates in all the tested varieties. It must also be noted that emodins, free reducing sugars, anthocyanins, coumarins, and betacyanins were not detected in any of the N. tabacum tested varieties.

Although it is widely accepted that the phytochemical composition of N. tabacum may vary depending upon processing conditions, temperature, maturation stage, time of harvesting, and storage [31,32], these findings are in agreement with a previous work by Shekins et al., who reported the presence of glycosides, alkaloids, tannins, phenols, and reducing sugars in methanolic extracts of N. tabacum [33].

Following the previously reported promising results concerning the antibiotic potential of N. tabacum extracts [18], the antibacterial activity of N. tabacum methanolic extracts against B. subtilis, E. coli, S. aureus, and S. typhi was tested using the well diffusion method. In this study, ampicillin was used as positive control, and the results are reported in Figure 2 and Table S2 in the Supporting information. The highest antibacterial activity in terms of zone of inhibition (17.0 ± 0.3 mm) was observed in the case of K399 extract against E. coli, while SN2 extract was the least effective against the same strain (7.5 ± 0.6 mm). Nevertheless, overall, all the extracts from the four different varieties of N. tabacum possessed significant antibacterial activity against the experimented strains.

Figure 2 Antibacterial activity of N. tabacum SPG38, K399, SN1, and SN2 varieties against various bacterial strains.
Figure 2

Antibacterial activity of N. tabacum SPG38, K399, SN1, and SN2 varieties against various bacterial strains.

The potential of N. tabacum constituents as antifungal agents was discussed in recent reports [19]. In this context, the extracts from the four varieties of N. tabacum were used to test the inhibitory zone towards fungi such as T. harzianum, A. brasiliensis, C. albicans, and A. niger. According to the results, reported in Figure 3 and Table S3 in the Supporting information, K399 again showed the highest zone of inhibition (16.5 ± 0.2 mm) against C. albicans but it was less effective against A. niger (7.0 ± 0.2 mm). These findings are in agreement with the observations by Jabeen et al., who showed that alcoholic extracts of N. tabacum possess highest antifungal property against A. flavus as compared to A. niger [34].

Figure 3 Antifungal activity of N. tabacum SPG38, K399, SN1, and SN2 varieties against various fungal strains.
Figure 3

Antifungal activity of N. tabacum SPG38, K399, SN1, and SN2 varieties against various fungal strains.

Then, we aimed at investigating a potential mechanism of action by which the extracts may exert their anti-infective activity. In this context, urease is a metalloenzyme that contributes to the regulation of nitrogen metabolism, as it hydrolyzes urea into ammonia and carbamate. Most importantly, it is conserved in many organisms among plants, bacteria, and fungi [35]. Additionally, urease is a target investigated, also by means of computational tools, in the field of drug discovery applied to anti-infective agents [36].

The calculated IC50 values of the extracts as jack bean urease inhibitors for K399, SPG28, SN1, and SN2 were 37.09 ± 2.76, 22.87 ± 1.54, 41.98 ± 2.01, and 10.11 ± 0.98 μg/mL, respectively. For thiourea, which was used as a positive control, an IC50 value of 21.54 ± 1.00 μg/mL was calculated. On the other hand, the urease inhibitory potential of SPG28 extract was the highest at 200 µg/mL (highest concentration tested). The results, listed in Table 1, demonstrate that the extract inhibit the enzyme, and that the lowest IC50 value was observed for SN2 variety.

Table 1

Urease inhibitory activity of N. tabacum extracts

Extract/standard % Inhibition at 200 µg/mL IC50 (µg/mL)
K399 75.32 37.09 ± 2.76
SPG28 89.43 22.87 ± 1.54
SN1 69.32 41.98 ± 2.01
SN2 91.54 10.11 ± 0.98
Thiourea 98.45 21.54 ± 1.00

Eventually, we carried out a preliminary computational study to investigate the binding mode to the same macromolecular target examined in vitro of two highly representative components of the polyphenolic fraction of N. tabacum leaves, namely, rutin and chlorogenic acid [12–14]. These compounds have been widely investigated for their multitarget activity [37–40]. Indeed, computational tools are nowadays widely used in the drug discovery workflow [41,42]. Docking studies showed that rutin and chlorogenic acid may interact with the active site of urease, in close proximity to the metal ions within the protein (Figure 4a). More specifically, a more promising calculated binding energy values was calculated for the first compound (−8.2 kcal/mol) when compared to the latter (−7.1 kcal/mol). A detailed view of the predicted interaction patterns with the enzyme is reported in Figure 4b and c.

Figure 4 Computed binding mode for rutin (orange) and chlorogenic acid (green) with urease (PDB ID 3LA4) (a) A detailed view of the residues interacting with the natural compounds is depicted for rutin (b) and chlorogenic acid (c). The involved amino acids are highlighted in blue.
Figure 4

Computed binding mode for rutin (orange) and chlorogenic acid (green) with urease (PDB ID 3LA4) (a) A detailed view of the residues interacting with the natural compounds is depicted for rutin (b) and chlorogenic acid (c). The involved amino acids are highlighted in blue.

4 Conclusion

In this study, the presence of bioactive components in N. tabacum K399, SPG28, SN1, and SN2 extracts was highlighted. The four varieties contain alkaloids, flavonoids, polyphenols, and carbohydrates, plus some classes of compounds that specifically characterize the single varieties.

In vitro studies demonstrated that N. tabacum extracts, and K399 in particular, possess significant antibacterial and antifungal activities. Besides, enzymatic inhibition and computational studies support the hypothesis that urease inhibition is involved in the observed biological effect.

Taken together, the computational and experimental studies support the role of N. tabacum extracts as anti-infective agents, in a field of research in which the identification of novel remedies is an urgent need [15,43].

Although further studies are needed to clarify the involvement of single compounds at the molecular level, these findings pave the way for the identification of novel classes of nature-inspired anti-infective agents from N. tabacum leaves.

Acknowledgments

Authors are thankful to Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2023R47), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

  1. Funding information: G.R. acknowledges funding from University of Brescia. Authors are thankful to Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2023R47), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabi.

  2. Author contributions: Literature search: G.R.; experimental studies: M.H.K., M.S., A.A.K., N.A., R.S., and G.R.; data analysis: M.H.K., M.S., A.A.K., N.A., R.S., and G.R.; manuscript preparation and manuscript revision: A.R., T.A.S., and R.S.

  3. Conflict of interest: Prof. Abdur Rauf is a member of the Editorial Board of Open Chemistry. The other authors declare no conflict of interest.

  4. Ethical approval: The conducted research is not related to either human or animal use.

  5. Data availability statement: All data generated or analyzed during this study are included in this published article.

References

[1] Briški F, Horgas N, Vuković M, Gomzi Z. Aerobic composting of tobacco industry solid waste—simulation of the process. Clean Technol Environ Policy. 2003;5(3–4):295–301.10.1007/s10098-003-0218-7Search in Google Scholar

[2] Leal M, Moreno MA, Albornoz PL, Mercado MI, Zampini IC, Isla MI. Nicotiana tabacum leaf waste: Morphological characterization and chemical-functional analysis of extracts obtained from powder leaves by using green solvents. Molecules. 2023;28(3):1396.10.3390/molecules28031396Search in Google Scholar PubMed PubMed Central

[3] Fatica A, Di Lucia F, Marino S, Alvino A, Zuin M, De Feijter H, et al. Study on analytical characteristics of Nicotiana tabacum L., cv. Solaris biomass for potential uses in nutrition and biomethane production. Sci Rep. 2019;9(1):16828.10.1038/s41598-019-53237-8Search in Google Scholar PubMed PubMed Central

[4] Hossain AM, Salehuddin SM. Analytical determination of nicotine in tobacco leaves by gas chromatography–mass spectrometry. Arab J Chem. 2013;6(3):275–8.10.1016/j.arabjc.2010.10.006Search in Google Scholar

[5] Mehmood F, Abdullah, Ubaid Z, Shahzadi I, Ahmed I, Waheed MT, et al. Plastid genomics of Nicotiana (Solanaceae): insights into molecular evolution, positive selection and the origin of the maternal genome of Aztec tobacco ( Nicotiana rustica). PeerJ. 2020;8:e9552.10.7717/peerj.9552Search in Google Scholar PubMed PubMed Central

[6] Zou X, Bk A, Abu-Izneid T, Aziz A, Devnath P, Rauf A, et al. Current advances of functional phytochemicals in Nicotiana plant and related potential value of tobacco processing waste: A review. Biomed Pharmacother. 2021;143:112191.10.1016/j.biopha.2021.112191Search in Google Scholar PubMed

[7] Laszlo C, Kaminski K, Guan H, Fatarova M, Wei J, Bergounioux A, et al. Fractionation and extraction optimization of potentially valuable compounds and their profiling in six varieties of two nicotiana species. Molecules. 2022;27(22):8105.10.3390/molecules27228105Search in Google Scholar PubMed PubMed Central

[8] Jassbi AR, Zare S, Asadollahi M, Schuman MC. Ecological roles and biological activities of specialized metabolites from the genus Nicotiana. Chem Rev. 2017;117(19):12227–80.10.1021/acs.chemrev.7b00001Search in Google Scholar PubMed

[9] Banožić M, Babić J, Jokić S. Recent advances in extraction of bioactive compounds from tobacco industrial waste - a review. Ind Crop Products. 2020;144:112009.10.1016/j.indcrop.2019.112009Search in Google Scholar

[10] Shen J, Shao X. Determination of tobacco alkaloids by gas chromatography–mass spectrometry using cloud point extraction as a preconcentration step. Anal Chim Acta. 2006;561(1–2):83–7.10.1016/j.aca.2006.01.002Search in Google Scholar

[11] Zou X, Bk A, Rauf A, Saeed M, Al-Awthan YS, A. Al-Duais M, et al. Screening of polyphenols in tobacco (Nicotiana tabacum) and determination of their antioxidant activity in different tobacco varieties. ACS Omega. 2021;6(39):25361–71.10.1021/acsomega.1c03275Search in Google Scholar PubMed PubMed Central

[12] Kurt D. Impacts of environmental variations on quality and chemical contents of oriental tobacco. Contrib Tob Nicotine Res. 2021;30(1):50–62.10.2478/cttr-2021-0006Search in Google Scholar

[13] Xie F, Yu A, Hou D, Liu H, Ding L, Zhang S. Rapid and sensitive analysis of eight polyphenols in tobacco by rapid resolution liquid chromatogarphy. AJAC. 2011;2(8):929–33.10.4236/ajac.2011.28107Search in Google Scholar

[14] Chittasupho C, Chaobankrang K, Sarawungkad A, Samee W, Singh S, Hemsuwimon K, et al. Antioxidant, anti-inflammatory and attenuating intracellular reactive oxygen species activities of Nicotiana tabacum var. Virginia leaf extract phytosomes and shape memory gel formulation. Gels. 2023;9(2):78.10.3390/gels9020078Search in Google Scholar PubMed PubMed Central

[15] Hobson C, Chan AN, Wright GD. The antibiotic resistome: A guide for the discovery of natural products as antimicrobial agents. Chem Rev. 2021;121(6):3464–94.10.1021/acs.chemrev.0c01214Search in Google Scholar PubMed

[16] Vengurlekar S, Sharma R, Trivedi P. Efficacy of some natural compounds as antifungal agents. Pharmacogn Rev. 2012;6(12):91.10.4103/0973-7847.99942Search in Google Scholar PubMed PubMed Central

[17] Hamaamin Hussen N, Hameed Hasan A, Jamalis J, Shakya S, Chander S, Kharkwal H, et al. Potential inhibitory activity of phytoconstituents against black fungus: In silico ADMET, molecular docking and MD simulation studies. Comput Toxicol. 2022;24:100247.10.1016/j.comtox.2022.100247Search in Google Scholar PubMed PubMed Central

[18] Ameya G, Manilal A, Merdekios B. In vitro antibacterial activity and phytochemical analysis of Nicotiana tabacum L. extracted in different organic solvents. Open Microbiol J. 2017;11(1):352–9.10.2174/1874285801711010352Search in Google Scholar PubMed PubMed Central

[19] Yang YL, Song BQ, Long JY, Fang DH, Hu DB, Song N, et al. Antifungal constituents from Nicotiana tabacum with the Wz locus infected by Phytophthora nicotianae. Results Chem. 2021;3:100196.10.1016/j.rechem.2021.100196Search in Google Scholar

[20] Flores JS, Ricalde RV. The secretions and exudates of plants used in Mayan traditional medicine. J Herbs Spices Med Plants. 1996;4(1):53–9.10.1300/J044v04n01_07Search in Google Scholar

[21] Bawazeer S, Rauf A, Nawaz T, Khalil AA, Javed MS, Muhammad N, et al. Punica granatum peel extracts mediated the green synthesis of gold nanoparticles and their detailed in vivo biological activities. Green Process Synth. 2021;10(1):882–92.10.1515/gps-2021-0080Search in Google Scholar

[22] Sharma R, Amin H, Galib, Prajapati PK. Validation of standard manufacturing procedure of Guḍūcī sattva (aqueous extract of Tinospora cordifolia (Willd.) Miers) and its tablets. Anc Sci Life. 2013;33(1):27–34.10.4103/0257-7941.134564Search in Google Scholar PubMed PubMed Central

[23] Prommaban A, Kheawfu K, Chittasupho C, Sirilun S, Hemsuwimon K, Chaiyana W. Phytochemical, antioxidant, antihyaluronidase, antityrosinase, and antimicrobial properties of Nicotiana tabacum L. leaf extracts. N Parajuli, curatore. Evid-Based Complement Altern Med. 2022;2022:1–11.10.1155/2022/5761764Search in Google Scholar PubMed PubMed Central

[24] Bawazeer S, Khan I, Rauf A, Aljohani ASM, Alhumaydhi FA, Khalil AA, et al. Black pepper (Piper nigrum) fruit-based gold nanoparticles (BP-AuNPs): Synthesis, characterization, biological activities, and catalytic applications – A green approach. Green Process Synth. 2022;11(1):11–28.10.1515/gps-2022-0002Search in Google Scholar

[25] Alhumaydhi FA, Khan I, Rauf A, Qureshi MN, Aljohani ASM, Khan SA, et al. Synthesis, characterization, biological activities, and catalytic applications of alcoholic extract of saffron (Crocus sativus) flower stigma-based gold nanoparticles. Green Process Synth. 2021;10(1):230–45.10.1515/gps-2021-0024Search in Google Scholar

[26] Saeed A, Mahmood SU, Rafiq M, Ashraf Z, Jabeen F, Seo SY. Iminothiazoline-sulfonamide hybrids as jack bean urease inhibitors; synthesis, kinetic mechanism and computational molecular modeling. Chem Biol Drug Des. 2016;87(3):434–43.10.1111/cbdd.12675Search in Google Scholar PubMed

[27] Imran M, Waqar S, Ogata K, Ahmed M, Noreen Z, Javed S, et al. Identification of novel bacterial urease inhibitors through molecular shape and structure based virtual screening approaches. RSC Adv. 2020;10(27):16061–70.10.1039/D0RA02363ASearch in Google Scholar PubMed PubMed Central

[28] Hanwell MD, Curtis DE, Lonie DC, Vandermeerschd T, Zurek E, Hutchison GR. Avogadro: An advanced semantic chemical editor, visualization, and analysis platform. J Cheminform. 2012;4:1–17.10.1186/1758-2946-4-17Search in Google Scholar PubMed PubMed Central

[29] Trott O, Olson AJ. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2010;31(2):455–61.10.1002/jcc.21334Search in Google Scholar PubMed PubMed Central

[30] Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, et al. UCSF chimera? A visualization system for exploratory research and analysis. J Comput Chem. 2004;25(13):1605–12.10.1002/jcc.20084Search in Google Scholar PubMed

[31] Fu B, Ji X, Zhao M, He F, Wang X, Wang Y, et al. The influence of light quality on the accumulation of flavonoids in tobacco ( Nicotiana tabacum L.) leaves. J Photochem Photobiol B Biol. 2016;162:544–9.10.1016/j.jphotobiol.2016.07.016Search in Google Scholar PubMed

[32] Popova V, Gochev V, Girova T, Iliev I, Ivanova T, Stoyanova A. Extraction products from tobacco – aroma and bioactive compounds and activities. CBC. 2015;11(1):31–7.10.2174/157340721101150804150016Search in Google Scholar

[33] Shekins O, Dorathy E, Labaran M, Joel P. Phytochemical screening of tobacco (Nicotiana tabacum) and its effects on some haematological parameters and histopathology of liver and brain in male rats. IJBCRR. 2016;14(4):1–9.10.9734/IJBCRR/2016/29645Search in Google Scholar

[34] Jabeen K, Naeem Z, Maimoona A, Khan B, Iqbal S. Evaluation of fungicidal potential of Ocimum sanctum L. and Nicotiana tabacum L. against Aspergillus flavus and Aspergillus niger. PAKJBOT [Internet]. 2020;52(6). [citato 4 aprile 2023]. http://pakbs.org/pjbot/paper_details.php?id=8580.10.30848/PJB2020-6(8)Search in Google Scholar

[35] Kappaun K, Piovesan AR, Carlini CR, Ligabue-Braun R. Ureases: Historical aspects, catalytic, and non-catalytic properties – A review. J Adv Res. 2018;13:3–17.10.1016/j.jare.2018.05.010Search in Google Scholar PubMed PubMed Central

[36] Aniceto N, Bonifácio VDB, Guedes RC, Martinho N. Exploring the chemical space of urease inhibitors to extract meaningful trends and drivers of activity. J Chem Inf Model. 2022;62(15):3535–50.10.1021/acs.jcim.2c00150Search in Google Scholar PubMed PubMed Central

[37] Ribaudo G, Memo M, Gianoncelli A. Multi-target natural and nature-inspired compounds against neurodegeneration: A focus on dual cholinesterase and phosphodiesterase inhibitors. Appl Sci. 2021;11(11):5044.10.3390/app11115044Search in Google Scholar

[38] Ongaro A, Zagotto G, Memo M, Gianoncelli A, Ribaudo G. Natural phosphodiesterase 5 (PDE5) inhibitors: A computational approach. Nat Product Res. 2021;35(10):1648–53.10.1080/14786419.2019.1619726Search in Google Scholar PubMed

[39] Ribaudo G, Oselladore E, Ongaro A, Zagotto G, Memo M, Gianoncelli A. Enhanced G-quadruplex selectivity of flavonoid glycoside rutin over quercetin. Nat Product Res. 2022;36(13):3469–73.Search in Google Scholar

[40] Islam MdR, Rahman MdM, Dhar PS, Nowrin FT, Sultana N, Akter M, et al. The role of natural and semi-synthetic compounds in ovarian cancer: Updates on mechanisms of action, current trends and perspectives. Molecules. 2023;28(5):2070.10.3390/molecules28052070Search in Google Scholar PubMed PubMed Central

[41] Valentin Y, Fail G, Pavel U. Shapley values to explain machine learning models of school student’s academic performance during COVID-19. 3C TIC. 2022;11(2):136–44.10.17993/3ctic.2022.112.136-144Search in Google Scholar

[42] Bawankar BU, Chinnaiah K. Implementation of ensemble method on DNA data using various cross validation techniques. 3C Tecnología. 2022;11(2):59–69.10.17993/3ctecno.2022.v11n2e42.59-69Search in Google Scholar

[43] Qu L, Chen Z. A mathematical model of plasmid-carried antibiotic resistance transmission in two types of cells. Appl Math Nonlinear Sci. 2022. https://doi.org/10.2478/amns.2021.2.00178.10.2478/amns.2021.2.00178Search in Google Scholar
Received: 2023-05-01
Revised: 2023-05-22
Accepted: 2023-05-23
Published Online: 2023-06-20

© 2023 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  4. Pomegranate attenuates kidney injury in cyclosporine-induced nephrotoxicity in rats by suppressing oxidative stress
  5. Ab initio study of fundamental properties of XInO3 (X = K, Rb, Cs) perovskites
  6. Responses of feldspathic sandstone and sand-reconstituted soil C and N to freeze–thaw cycles
  7. Robust fractional control based on high gain observers design (RNFC) for a Spirulina maxima culture interfaced with an advanced oxidation process
  8. Study on arsenic speciation and redistribution mechanism in Lonicera japonica plants via synchrotron techniques
  9. Optimization of machining Nilo 36 superalloy parameters in turning operation
  10. Vacuum impregnation pre-treatment: A novel method for incorporating mono- and divalent cations into potato strips to reduce the acrylamide formation in French fries
  11. Characterization of effective constituents in Acanthopanax senticosus fruit for blood deficiency syndrome based on the chinmedomics strategy
  12. Comparative analysis of the metabolites in Pinellia ternata from two producing regions using ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry
  13. The assessment of environmental parameter along the desalination plants in the Kingdom of Saudi Arabia
  14. Effects of harpin and carbendazim on antioxidant accumulation in young jujube leaves
  15. The effects of in ovo injected with sodium borate on hatching performance and small intestine morphology in broiler chicks
  16. Optimization of cutting forces and surface roughness via ANOVA and grey relational analysis in machining of In718
  17. Essential oils of Origanum compactum Benth: Chemical characterization, in vitro, in silico, antioxidant, and antibacterial activities
  18. Translocation of tungsten(vi) oxide/gadolinium(iii) fluoride in tellurite glasses towards improvement of gamma-ray attenuation features in high-density glass shields
  19. Mechanical properties, elastic moduli, and gamma ray attenuation competencies of some TeO2–WO3–GdF3 glasses: Tailoring WO3–GdF3 substitution toward optimum behavioral state range
  20. Comparison between the CIDR or sponge with hormone injection to induce estrus synchronization for twining and sex preselection in Naimi sheep
  21. Exergetic performance analyses of three different cogeneration plants
  22. Psoralea corylifolia (babchi) seeds enhance proliferation of normal human cultured melanocytes: GC–MS profiling and biological investigation
  23. A novel electrochemical micro-titration method for quantitative evaluation of the DPPH free radical scavenging capacity of caffeic acid
  24. Comparative study between supported bimetallic catalysts for nitrate remediation in water
  25. Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells
  26. Determination of nicotine content in locally produced smokeless tobacco (Shammah) samples from Jazan region of Saudi Arabia using a convenient HPLC-MS/MS method
  27. Changes in oxidative stress markers in pediatric burn injury over a 1-week period
  28. Integrated geophysical techniques applied for petroleum basins structural characterization in the central part of the Western Desert, Egypt
  29. The impact of chemical modifications on gamma-ray attenuation properties of some WO3-reinforced tellurite glasses
  30. Microwave and Cs+-assisted chemo selective reaction protocol for synthesizing 2-styryl quinoline biorelevant molecules
  31. Structural, physical, and radiation absorption properties of a significant nuclear power plant component: A comparison between REX-734 and 316L SS austenitic stainless steels
  32. Effect of Moringa oleifera on serum YKL-40 level: In vivo rat periodontitis model
  33. Investigating the impact of CO2 emissions on the COVID-19 pandemic by generalized linear mixed model approach with inverse Gaussian and gamma distributions
  34. Influence of WO3 content on gamma rays attenuation characteristics of phosphate glasses at low energy range
  35. Study on CO2 absorption performance of ternary DES formed based on DEA as promoting factor
  36. Performance analyses of detonation engine cogeneration cycles
  37. Sterols from Centaurea pumilio L. with cell proliferative activity: In vitro and in silico studies
  38. Untargeted metabolomics revealing changes in aroma substances in flue-cured tobacco
  39. Effect of pumpkin enriched with calcium lactate on iron status in an animal model of postmenopausal osteoporosis
  40. Energy consumption, mechanical and metallographic properties of cryogenically treated tool steels
  41. Optimization of ultra-high pressure-assisted extraction of total phenols from Eucommia ulmoides leaves by response surface methodology
  42. Harpin enhances antioxidant nutrient accumulation and decreases enzymatic browning in stored soybean sprouts
  43. Physicochemical and biological properties of carvacrol
  44. Radix puerariae in the treatment of diabetic nephropathy: A network pharmacology analysis and experimental validation
  45. Anti-Alzheimer, antioxidants, glucose-6-phosphate dehydrogenase effects of Taverniera glabra mediated ZnO and Fe2O3 nanoparticles in alloxan-induced diabetic rats
  46. Experimental study on photocatalytic CO2 reduction performance of ZnS/CdS-TiO2 nanotube array thin films
  47. Epoxy-reinforced heavy metal oxides for gamma ray shielding purposes
  48. Black mulberry (Morus nigra L.) fruits: As a medicinal plant rich in human health-promoting compounds
  49. Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations
  50. Chloramine-T-induced oxidation of Rizatriptan Benzoate: An integral chemical and spectroscopic study of products, mechanisms and kinetics
  51. Study on antioxidant and antimicrobial potential of chemically profiled essential oils extracted from Juniperus phoenicea (L.) by use of in vitro and in silico approaches
  52. Screening and characterization of fungal taxol-producing endophytic fungi for evaluation of antimicrobial and anticancer activities
  53. Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts
  54. In vitro antiproliferative efficacy of Annona muricata seed and fruit extracts on several cancer cell lines
  55. An experimental study for chemical characterization of artificial anterior cruciate ligament with coated chitosan as biomaterial
  56. Prevalence of residual risks of the transfusion-transmitted infections in Riyadh hospitals: A two-year retrospective study
  57. Computational and experimental investigation of antibacterial and antifungal properties of Nicotiana tabacum extracts
  58. Reinforcement of cementitious mortars with hemp fibers and shives
  59. X-ray shielding properties of bismuth-borate glass doped with rare earth ions
  60. Green supported silver nanoparticles over modified reduced graphene oxide: Investigation of its antioxidant and anti-ovarian cancer effects
  61. Orthogonal synthesis of a versatile building block for dual functionalization of targeting vectors
  62. Thymbra spicata leaf extract driven biogenic synthesis of Au/Fe3O4 nanocomposite and its bio-application in the treatment of different types of leukemia
  63. The role of Ag2O incorporation in nuclear radiation shielding behaviors of the Li2O–Pb3O4–SiO2 glass system: A multi-step characterization study
  64. A stimuli-responsive in situ spray hydrogel co-loaded with naringenin and gentamicin for chronic wounds
  65. Assessment of the impact of γ-irradiation on the piperine content and microbial quality of black pepper
  66. Antioxidant, sensory, and functional properties of low-alcoholic IPA beer with Pinus sylvestris L. shoots addition fermented using unconventional yeast
  67. Screening and optimization of extracellular pectinase produced by Bacillus thuringiensis SH7
  68. Determination of polyphenols in Chinese jujube using ultra-performance liquid chromatography–mass spectrometry
  69. Synergistic effects of harpin and NaCl in determining soybean sprout quality under non-sterile conditions
  70. Field evaluation of different eco-friendly alternative control methods against Panonychus citri [Acari: Tetranychidae] spider mite and its predators in citrus orchards
  71. Exploring the antimicrobial potential of biologically synthesized zero valent iron nanoparticles
  72. NaCl regulates goldfish growth and survival at three food supply levels under hypoxia
  73. An exploration of the physical, optical, mechanical, and radiation shielding properties of PbO–MgO–ZnO–B2O3 glasses
  74. A novel statistical modeling of air pollution and the COVID-19 pandemic mortality data by Poisson, geometric, and negative binomial regression models with fixed and random effects
  75. Treatment activity of the injectable hydrogels loaded with dexamethasone In(iii) complex on glioma by inhibiting the VEGF signaling pathway
  76. An alternative approach for the excess lifetime cancer risk and prediction of radiological parameters
  77. Panax ginseng leaf aqueous extract mediated green synthesis of AgNPs under ultrasound condition and investigation of its anti-lung adenocarcinoma effects
  78. Study of hydrolysis and production of instant ginger (Zingiber officinale) tea
  79. Novel green synthesis of zinc oxide nanoparticles using Salvia rosmarinus extract for treatment of human lung cancer
  80. Evaluation of second trimester plasma lipoxin A4, VEGFR-1, IL-6, and TNF-α levels in pregnant women with gestational diabetes mellitus
  81. Antidiabetic, antioxidant and cytotoxicity activities of ortho- and para-substituted Schiff bases derived from metformin hydrochloride: Validation by molecular docking and in silico ADME studies
  82. Antioxidant, antidiabetic, antiglaucoma, and anticholinergic effects of Tayfi grape (Vitis vinifera): A phytochemical screening by LC-MS/MS analysis
  83. Identification of genetic polymorphisms in the stearoyl CoA desaturase gene and its association with milk quality traits in Najdi sheep
  84. Cold-acclimation effect on cadmium absorption and biosynthesis of polyphenolics, and free proline and photosynthetic pigments in Spirogyra aequinoctialis
  85. Analysis of secondary metabolites in Xinjiang Morus nigra leaves using different extraction methods with UPLC-Q/TOF-MS/MS technology
  86. Nanoarchitectonics and performance evaluation of a Fe3O4-stabilized Pickering emulsion-type differential pressure plugging agent
  87. Investigating pyrolysis characteristics of Shengdong coal through Py-GC/MS
  88. Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract
  89. Introducing a novel and natural antibiotic for the treatment of oral pathogens: Abelmoschus esculentus green-formulated silver nanoparticles
  90. Optimization of gallic acid-enriched ultrasonic-assisted extraction from mango peels
  91. Effect of gamma rays irradiation in the structure, optical, and electrical properties of samarium doped bismuth titanate ceramics
  92. Combinatory in silico investigation for potential inhibitors from Curcuma sahuynhensis Škorničk. & N.S. Lý volatile phytoconstituents against influenza A hemagglutinin, SARS-CoV-2 main protease, and Omicron-variant spike protein
  93. Physical, mechanical, and gamma ray shielding properties of the Bi2O3–BaO–B2O3–ZnO–As2O3–MgO–Na2O glass system
  94. Twofold interpenetrated 3D Cd(ii) complex: Crystal structure and luminescent property
  95. Study on the microstructure and soil quality variation of composite soil with soft rock and sand
  96. Ancient spring waters still emerging and accessible in the Roman Forum area: Chemical–physical and microbiological characterization
  97. Extraction and characterization of type I collagen from scales of Mexican Biajaiba fish
  98. Finding small molecular compounds to decrease trimethylamine oxide levels in atherosclerosis by virtual screening
  99. Prefatory in silico studies and in vitro insecticidal effect of Nigella sativa (L.) essential oil and its active compound (carvacrol) against the Callosobruchus maculatus adults (Fab), a major pest of chickpea
  100. Polymerized methyl imidazole silver bromide (CH3C6H5AgBr)6: Synthesis, crystal structures, and catalytic activity
  101. Using calcined waste fish bones as a green solid catalyst for biodiesel production from date seed oil
  102. Influence of the addition of WO3 on TeO2–Na2O glass systems in view of the feature of mechanical, optical, and photon attenuation
  103. Naringin ameliorates 5-fluorouracil elicited neurotoxicity by curtailing oxidative stress and iNOS/NF-ĸB/caspase-3 pathway
  104. GC-MS profile of extracts of an endophytic fungus Alternaria and evaluation of its anticancer and antibacterial potentialities
  105. Green synthesis, chemical characterization, and antioxidant and anti-colorectal cancer effects of vanadium nanoparticles
  106. Determination of caffeine content in coffee drinks prepared in some coffee shops in the local market in Jeddah City, Saudi Arabia
  107. A new 3D supramolecular Cu(ii) framework: Crystal structure and photocatalytic characteristics
  108. Bordeaux mixture accelerates ripening, delays senescence, and promotes metabolite accumulation in jujube fruit
  109. Important application value of injectable hydrogels loaded with omeprazole Schiff base complex in the treatment of pancreatitis
  110. Color tunable benzothiadiazole-based small molecules for lightening applications
  111. Investigation of structural, dielectric, impedance, and mechanical properties of hydroxyapatite-modified barium titanate composites for biomedical applications
  112. Metal gel particles loaded with epidermal cell growth factor promote skin wound repair mechanism by regulating miRNA
  113. In vitro exploration of Hypsizygus ulmarius (Bull.) mushroom fruiting bodies: Potential antidiabetic and anti-inflammatory agent
  114. Alteration in the molecular structure of the adenine base exposed to gamma irradiation: An ESR study
  115. Comprehensive study of optical, thermal, and gamma-ray shielding properties of Bi2O3–ZnO–PbO–B2O3 glasses
  116. Lewis acids as co-catalysts in Pd-based catalyzed systems of the octene-1 hydroethoxycarbonylation reaction
  117. Synthesis, Hirshfeld surface analysis, thermal, and selective α-glucosidase inhibitory studies of Schiff base transition metal complexes
  118. Protective properties of AgNPs green-synthesized by Abelmoschus esculentus on retinal damage on the virtue of its anti-inflammatory and antioxidant effects in diabetic rat
  119. Effects of green decorated AgNPs on lignin-modified magnetic nanoparticles mediated by Cydonia on cecal ligation and puncture-induced sepsis
  120. Treatment of gastric cancer by green mediated silver nanoparticles using Pistacia atlantica bark aqueous extract
  121. Preparation of newly developed porcelain ceramics containing WO3 nanoparticles for radiation shielding applications
  122. Utilization of computational methods for the identification of new natural inhibitors of human neutrophil elastase in inflammation therapy
  123. Some anticancer agents as effective glutathione S-transferase (GST) inhibitors
  124. Clay-based bricks’ rich illite mineral for gamma-ray shielding applications: An experimental evaluation of the effect of pressure rates on gamma-ray attenuation parameters
  125. Stability kinetics of orevactaene pigments produced by Epicoccum nigrum in solid-state fermentation
  126. Treatment of denture stomatitis using iron nanoparticles green-synthesized by Silybum marianum extract
  127. Characterization and antioxidant potential of white mustard (Brassica hirta) leaf extract and stabilization of sunflower oil
  128. Characteristics of Langmuir monomolecular monolayers formed by the novel oil blends
  129. Strategies for optimizing the single GdSrFeO4 phase synthesis
  130. Oleic acid and linoleic acid nanosomes boost immunity and provoke cell death via the upregulation of beta-defensin-4 at genetic and epigenetic levels
  131. Unraveling the therapeutic potential of Bombax ceiba roots: A comprehensive study of chemical composition, heavy metal content, antibacterial activity, and in silico analysis
  132. Green synthesis of AgNPs using plant extract and investigation of its anti-human colorectal cancer application
  133. The adsorption of naproxen on adsorbents obtained from pepper stalk extract by green synthesis
  134. Treatment of gastric cancer by silver nanoparticles encapsulated by chitosan polymers mediated by Pistacia atlantica extract under ultrasound condition
  135. In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile
  136. Wear and corrosion behavior of TiC and WC coatings deposited on high-speed steels by electro-spark deposition
  137. Therapeutic effects of green-formulated gold nanoparticles by Origanum majorana on spinal cord injury in rats
  138. Melanin antibacterial activity of two new strains, SN1 and SN2, of Exophiala phaeomuriformis against five human pathogens
  139. Evaluation of the analgesic and anesthetic properties of silver nanoparticles supported over biodegradable acacia gum-modified magnetic nanoparticles
  140. Review Articles
  141. Role and mechanism of fruit waste polyphenols in diabetes management
  142. A comprehensive review of non-alkaloidal metabolites from the subfamily Amaryllidoideae (Amaryllidaceae)
  143. Discovery of the chemical constituents, structural characteristics, and pharmacological functions of Chinese caterpillar fungus
  144. Eco-friendly green approach of nickel oxide nanoparticles for biomedical applications
  145. Advances in the pharmaceutical research of curcumin for oral administration
  146. Rapid Communication
  147. Determination of the contents of bioactive compounds in St. John’s wort (Hypericum perforatum): Comparison of commercial and wild samples
  148. Retraction
  149. Retraction of “Two mixed-ligand coordination polymers based on 2,5-thiophenedicarboxylic acid and flexible N-donor ligands: The protective effect on periodontitis via reducing the release of IL-1β and TNF-α”
  150. Topical Issue on Phytochemicals, biological and toxicological analysis of aromatic medicinal plants
  151. Anti-plasmodial potential of selected medicinal plants and a compound Atropine isolated from Eucalyptus obliqua
  152. Anthocyanin extract from black rice attenuates chronic inflammation in DSS-induced colitis mouse model by modulating the gut microbiota
  153. Evaluation of antibiofilm and cytotoxicity effect of Rumex vesicarius methanol extract
  154. Chemical compositions of Litsea umbellata and inhibition activities
  155. Green synthesis, characterization of silver nanoparticles using Rhynchosia capitata leaf extract and their biological activities
  156. GC-MS analysis and antibacterial activities of some plants belonging to the genus Euphorbia on selected bacterial isolates
  157. The abrogative effect of propolis on acrylamide-induced toxicity in male albino rats: Histological study
  158. A phytoconstituent 6-aminoflavone ameliorates lipopolysaccharide-induced oxidative stress mediated synapse and memory dysfunction via p-Akt/NF-kB pathway in albino mice
  159. Anti-diabetic potentials of Sorbaria tomentosa Lindl. Rehder: Phytochemistry (GC-MS analysis), α-amylase, α-glucosidase inhibitory, in vivo hypoglycemic, and biochemical analysis
  160. Assessment of cytotoxic and apoptotic activities of the Cassia angustifolia aqueous extract against SW480 colon cancer
  161. Biochemical analysis, antioxidant, and antibacterial efficacy of the bee propolis extract (Hymenoptera: Apis mellifera) against Staphylococcus aureus-induced infection in BALB/c mice: In vitro and in vivo study
  162. Assessment of essential elements and heavy metals in Saudi Arabian rice samples underwent various processing methods
  163. Two new compounds from leaves of Capparis dongvanensis (Sy, B. H. Quang & D. V. Hai) and inhibition activities
  164. Hydroxyquinoline sulfanilamide ameliorates STZ-induced hyperglycemia-mediated amyleoid beta burden and memory impairment in adult mice
  165. An automated reading of semi-quantitative hemagglutination results in microplates: Micro-assay for plant lectins
  166. Inductively coupled plasma mass spectrometry assessment of essential and toxic trace elements in traditional spices consumed by the population of the Middle Eastern region in their recipes
  167. Phytochemical analysis and anticancer activity of the Pithecellobium dulce seed extract in colorectal cancer cells
  168. Impact of climatic disturbances on the chemical compositions and metabolites of Salvia officinalis
  169. Physicochemical characterization, antioxidant and antifungal activities of essential oils of Urginea maritima and Allium sativum
  170. Phytochemical analysis and antifungal efficiency of Origanum majorana extracts against some phytopathogenic fungi causing tomato damping-off diseases
  171. Special Issue on 4th IC3PE
  172. Graphene quantum dots: A comprehensive overview
  173. Studies on the intercalation of calcium–aluminium layered double hydroxide-MCPA and its controlled release mechanism as a potential green herbicide
  174. Synergetic effect of adsorption and photocatalysis by zinc ferrite-anchored graphitic carbon nitride nanosheet for the removal of ciprofloxacin under visible light irradiation
  175. Exploring anticancer activity of the Indonesian guava leaf (Psidium guajava L.) fraction on various human cancer cell lines in an in vitro cell-based approach
  176. The comparison of gold extraction methods from the rock using thiourea and thiosulfate
  177. Special Issue on Marine environmental sciences and significance of the multidisciplinary approaches
  178. Sorption of alkylphenols and estrogens on microplastics in marine conditions
  179. Cytotoxic ketosteroids from the Red Sea soft coral Dendronephthya sp.
  180. Antibacterial and biofilm prevention metabolites from Acanthophora spicifera
  181. Characteristics, source, and health risk assessment of aerosol polyaromatic hydrocarbons in the rural and urban regions of western Saudi Arabia
  182. Special Issue on Advanced Nanomaterials for Energy, Environmental and Biological Applications - Part II
  183. Green synthesis, characterization, and evaluation of antibacterial activities of cobalt nanoparticles produced by marine fungal species Periconia prolifica
  184. Combustion-mediated sol–gel preparation of cobalt-doped ZnO nanohybrids for the degradation of acid red and antibacterial performance
  185. Perinatal supplementation with selenium nanoparticles modified with ascorbic acid improves hepatotoxicity in rat gestational diabetes
  186. Evaluation and chemical characterization of bioactive secondary metabolites from endophytic fungi associated with the ethnomedicinal plant Bergenia ciliata
  187. Enhancing photovoltaic efficiency with SQI-Br and SQI-I sensitizers: A comparative analysis
  188. Nanostructured p-PbS/p-CuO sulfide/oxide bilayer heterojunction as a promising photoelectrode for hydrogen gas generation
https://doi.org/10.1515/chem-2022-0343
Keywords for this article
Nicotiana tabacum; rutin; chlorogenic acid; antibacterial; antifungal
Creative Commons
BY 4.0

Articles in the same Issue

  1. Characteristics, source, and health risk assessment of aerosol polyaromatic hydrocarbons in the rural and urban regions of western Saudi Arabia
  2. Regular Articles
  3. A network-based correlation research between element electronegativity and node importance
  4. Pomegranate attenuates kidney injury in cyclosporine-induced nephrotoxicity in rats by suppressing oxidative stress
  5. Ab initio study of fundamental properties of XInO3 (X = K, Rb, Cs) perovskites
  6. Responses of feldspathic sandstone and sand-reconstituted soil C and N to freeze–thaw cycles
  7. Robust fractional control based on high gain observers design (RNFC) for a Spirulina maxima culture interfaced with an advanced oxidation process
  8. Study on arsenic speciation and redistribution mechanism in Lonicera japonica plants via synchrotron techniques
  9. Optimization of machining Nilo 36 superalloy parameters in turning operation
  10. Vacuum impregnation pre-treatment: A novel method for incorporating mono- and divalent cations into potato strips to reduce the acrylamide formation in French fries
  11. Characterization of effective constituents in Acanthopanax senticosus fruit for blood deficiency syndrome based on the chinmedomics strategy
  12. Comparative analysis of the metabolites in Pinellia ternata from two producing regions using ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry
  13. The assessment of environmental parameter along the desalination plants in the Kingdom of Saudi Arabia
  14. Effects of harpin and carbendazim on antioxidant accumulation in young jujube leaves
  15. The effects of in ovo injected with sodium borate on hatching performance and small intestine morphology in broiler chicks
  16. Optimization of cutting forces and surface roughness via ANOVA and grey relational analysis in machining of In718
  17. Essential oils of Origanum compactum Benth: Chemical characterization, in vitro, in silico, antioxidant, and antibacterial activities
  18. Translocation of tungsten(vi) oxide/gadolinium(iii) fluoride in tellurite glasses towards improvement of gamma-ray attenuation features in high-density glass shields
  19. Mechanical properties, elastic moduli, and gamma ray attenuation competencies of some TeO2–WO3–GdF3 glasses: Tailoring WO3–GdF3 substitution toward optimum behavioral state range
  20. Comparison between the CIDR or sponge with hormone injection to induce estrus synchronization for twining and sex preselection in Naimi sheep
  21. Exergetic performance analyses of three different cogeneration plants
  22. Psoralea corylifolia (babchi) seeds enhance proliferation of normal human cultured melanocytes: GC–MS profiling and biological investigation
  23. A novel electrochemical micro-titration method for quantitative evaluation of the DPPH free radical scavenging capacity of caffeic acid
  24. Comparative study between supported bimetallic catalysts for nitrate remediation in water
  25. Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells
  26. Determination of nicotine content in locally produced smokeless tobacco (Shammah) samples from Jazan region of Saudi Arabia using a convenient HPLC-MS/MS method
  27. Changes in oxidative stress markers in pediatric burn injury over a 1-week period
  28. Integrated geophysical techniques applied for petroleum basins structural characterization in the central part of the Western Desert, Egypt
  29. The impact of chemical modifications on gamma-ray attenuation properties of some WO3-reinforced tellurite glasses
  30. Microwave and Cs+-assisted chemo selective reaction protocol for synthesizing 2-styryl quinoline biorelevant molecules
  31. Structural, physical, and radiation absorption properties of a significant nuclear power plant component: A comparison between REX-734 and 316L SS austenitic stainless steels
  32. Effect of Moringa oleifera on serum YKL-40 level: In vivo rat periodontitis model
  33. Investigating the impact of CO2 emissions on the COVID-19 pandemic by generalized linear mixed model approach with inverse Gaussian and gamma distributions
  34. Influence of WO3 content on gamma rays attenuation characteristics of phosphate glasses at low energy range
  35. Study on CO2 absorption performance of ternary DES formed based on DEA as promoting factor
  36. Performance analyses of detonation engine cogeneration cycles
  37. Sterols from Centaurea pumilio L. with cell proliferative activity: In vitro and in silico studies
  38. Untargeted metabolomics revealing changes in aroma substances in flue-cured tobacco
  39. Effect of pumpkin enriched with calcium lactate on iron status in an animal model of postmenopausal osteoporosis
  40. Energy consumption, mechanical and metallographic properties of cryogenically treated tool steels
  41. Optimization of ultra-high pressure-assisted extraction of total phenols from Eucommia ulmoides leaves by response surface methodology
  42. Harpin enhances antioxidant nutrient accumulation and decreases enzymatic browning in stored soybean sprouts
  43. Physicochemical and biological properties of carvacrol
  44. Radix puerariae in the treatment of diabetic nephropathy: A network pharmacology analysis and experimental validation
  45. Anti-Alzheimer, antioxidants, glucose-6-phosphate dehydrogenase effects of Taverniera glabra mediated ZnO and Fe2O3 nanoparticles in alloxan-induced diabetic rats
  46. Experimental study on photocatalytic CO2 reduction performance of ZnS/CdS-TiO2 nanotube array thin films
  47. Epoxy-reinforced heavy metal oxides for gamma ray shielding purposes
  48. Black mulberry (Morus nigra L.) fruits: As a medicinal plant rich in human health-promoting compounds
  49. Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations
  50. Chloramine-T-induced oxidation of Rizatriptan Benzoate: An integral chemical and spectroscopic study of products, mechanisms and kinetics
  51. Study on antioxidant and antimicrobial potential of chemically profiled essential oils extracted from Juniperus phoenicea (L.) by use of in vitro and in silico approaches
  52. Screening and characterization of fungal taxol-producing endophytic fungi for evaluation of antimicrobial and anticancer activities
  53. Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts
  54. In vitro antiproliferative efficacy of Annona muricata seed and fruit extracts on several cancer cell lines
  55. An experimental study for chemical characterization of artificial anterior cruciate ligament with coated chitosan as biomaterial
  56. Prevalence of residual risks of the transfusion-transmitted infections in Riyadh hospitals: A two-year retrospective study
  57. Computational and experimental investigation of antibacterial and antifungal properties of Nicotiana tabacum extracts
  58. Reinforcement of cementitious mortars with hemp fibers and shives
  59. X-ray shielding properties of bismuth-borate glass doped with rare earth ions
  60. Green supported silver nanoparticles over modified reduced graphene oxide: Investigation of its antioxidant and anti-ovarian cancer effects
  61. Orthogonal synthesis of a versatile building block for dual functionalization of targeting vectors
  62. Thymbra spicata leaf extract driven biogenic synthesis of Au/Fe3O4 nanocomposite and its bio-application in the treatment of different types of leukemia
  63. The role of Ag2O incorporation in nuclear radiation shielding behaviors of the Li2O–Pb3O4–SiO2 glass system: A multi-step characterization study
  64. A stimuli-responsive in situ spray hydrogel co-loaded with naringenin and gentamicin for chronic wounds
  65. Assessment of the impact of γ-irradiation on the piperine content and microbial quality of black pepper
  66. Antioxidant, sensory, and functional properties of low-alcoholic IPA beer with Pinus sylvestris L. shoots addition fermented using unconventional yeast
  67. Screening and optimization of extracellular pectinase produced by Bacillus thuringiensis SH7
  68. Determination of polyphenols in Chinese jujube using ultra-performance liquid chromatography–mass spectrometry
  69. Synergistic effects of harpin and NaCl in determining soybean sprout quality under non-sterile conditions
  70. Field evaluation of different eco-friendly alternative control methods against Panonychus citri [Acari: Tetranychidae] spider mite and its predators in citrus orchards
  71. Exploring the antimicrobial potential of biologically synthesized zero valent iron nanoparticles
  72. NaCl regulates goldfish growth and survival at three food supply levels under hypoxia
  73. An exploration of the physical, optical, mechanical, and radiation shielding properties of PbO–MgO–ZnO–B2O3 glasses
  74. A novel statistical modeling of air pollution and the COVID-19 pandemic mortality data by Poisson, geometric, and negative binomial regression models with fixed and random effects
  75. Treatment activity of the injectable hydrogels loaded with dexamethasone In(iii) complex on glioma by inhibiting the VEGF signaling pathway
  76. An alternative approach for the excess lifetime cancer risk and prediction of radiological parameters
  77. Panax ginseng leaf aqueous extract mediated green synthesis of AgNPs under ultrasound condition and investigation of its anti-lung adenocarcinoma effects
  78. Study of hydrolysis and production of instant ginger (Zingiber officinale) tea
  79. Novel green synthesis of zinc oxide nanoparticles using Salvia rosmarinus extract for treatment of human lung cancer
  80. Evaluation of second trimester plasma lipoxin A4, VEGFR-1, IL-6, and TNF-α levels in pregnant women with gestational diabetes mellitus
  81. Antidiabetic, antioxidant and cytotoxicity activities of ortho- and para-substituted Schiff bases derived from metformin hydrochloride: Validation by molecular docking and in silico ADME studies
  82. Antioxidant, antidiabetic, antiglaucoma, and anticholinergic effects of Tayfi grape (Vitis vinifera): A phytochemical screening by LC-MS/MS analysis
  83. Identification of genetic polymorphisms in the stearoyl CoA desaturase gene and its association with milk quality traits in Najdi sheep
  84. Cold-acclimation effect on cadmium absorption and biosynthesis of polyphenolics, and free proline and photosynthetic pigments in Spirogyra aequinoctialis
  85. Analysis of secondary metabolites in Xinjiang Morus nigra leaves using different extraction methods with UPLC-Q/TOF-MS/MS technology
  86. Nanoarchitectonics and performance evaluation of a Fe3O4-stabilized Pickering emulsion-type differential pressure plugging agent
  87. Investigating pyrolysis characteristics of Shengdong coal through Py-GC/MS
  88. Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract
  89. Introducing a novel and natural antibiotic for the treatment of oral pathogens: Abelmoschus esculentus green-formulated silver nanoparticles
  90. Optimization of gallic acid-enriched ultrasonic-assisted extraction from mango peels
  91. Effect of gamma rays irradiation in the structure, optical, and electrical properties of samarium doped bismuth titanate ceramics
  92. Combinatory in silico investigation for potential inhibitors from Curcuma sahuynhensis Škorničk. & N.S. Lý volatile phytoconstituents against influenza A hemagglutinin, SARS-CoV-2 main protease, and Omicron-variant spike protein
  93. Physical, mechanical, and gamma ray shielding properties of the Bi2O3–BaO–B2O3–ZnO–As2O3–MgO–Na2O glass system
  94. Twofold interpenetrated 3D Cd(ii) complex: Crystal structure and luminescent property
  95. Study on the microstructure and soil quality variation of composite soil with soft rock and sand
  96. Ancient spring waters still emerging and accessible in the Roman Forum area: Chemical–physical and microbiological characterization
  97. Extraction and characterization of type I collagen from scales of Mexican Biajaiba fish
  98. Finding small molecular compounds to decrease trimethylamine oxide levels in atherosclerosis by virtual screening
  99. Prefatory in silico studies and in vitro insecticidal effect of Nigella sativa (L.) essential oil and its active compound (carvacrol) against the Callosobruchus maculatus adults (Fab), a major pest of chickpea
  100. Polymerized methyl imidazole silver bromide (CH3C6H5AgBr)6: Synthesis, crystal structures, and catalytic activity
  101. Using calcined waste fish bones as a green solid catalyst for biodiesel production from date seed oil
  102. Influence of the addition of WO3 on TeO2–Na2O glass systems in view of the feature of mechanical, optical, and photon attenuation
  103. Naringin ameliorates 5-fluorouracil elicited neurotoxicity by curtailing oxidative stress and iNOS/NF-ĸB/caspase-3 pathway
  104. GC-MS profile of extracts of an endophytic fungus Alternaria and evaluation of its anticancer and antibacterial potentialities
  105. Green synthesis, chemical characterization, and antioxidant and anti-colorectal cancer effects of vanadium nanoparticles
  106. Determination of caffeine content in coffee drinks prepared in some coffee shops in the local market in Jeddah City, Saudi Arabia
  107. A new 3D supramolecular Cu(ii) framework: Crystal structure and photocatalytic characteristics
  108. Bordeaux mixture accelerates ripening, delays senescence, and promotes metabolite accumulation in jujube fruit
  109. Important application value of injectable hydrogels loaded with omeprazole Schiff base complex in the treatment of pancreatitis
  110. Color tunable benzothiadiazole-based small molecules for lightening applications
  111. Investigation of structural, dielectric, impedance, and mechanical properties of hydroxyapatite-modified barium titanate composites for biomedical applications
  112. Metal gel particles loaded with epidermal cell growth factor promote skin wound repair mechanism by regulating miRNA
  113. In vitro exploration of Hypsizygus ulmarius (Bull.) mushroom fruiting bodies: Potential antidiabetic and anti-inflammatory agent
  114. Alteration in the molecular structure of the adenine base exposed to gamma irradiation: An ESR study
  115. Comprehensive study of optical, thermal, and gamma-ray shielding properties of Bi2O3–ZnO–PbO–B2O3 glasses
  116. Lewis acids as co-catalysts in Pd-based catalyzed systems of the octene-1 hydroethoxycarbonylation reaction
  117. Synthesis, Hirshfeld surface analysis, thermal, and selective α-glucosidase inhibitory studies of Schiff base transition metal complexes
  118. Protective properties of AgNPs green-synthesized by Abelmoschus esculentus on retinal damage on the virtue of its anti-inflammatory and antioxidant effects in diabetic rat
  119. Effects of green decorated AgNPs on lignin-modified magnetic nanoparticles mediated by Cydonia on cecal ligation and puncture-induced sepsis
  120. Treatment of gastric cancer by green mediated silver nanoparticles using Pistacia atlantica bark aqueous extract
  121. Preparation of newly developed porcelain ceramics containing WO3 nanoparticles for radiation shielding applications
  122. Utilization of computational methods for the identification of new natural inhibitors of human neutrophil elastase in inflammation therapy
  123. Some anticancer agents as effective glutathione S-transferase (GST) inhibitors
  124. Clay-based bricks’ rich illite mineral for gamma-ray shielding applications: An experimental evaluation of the effect of pressure rates on gamma-ray attenuation parameters
  125. Stability kinetics of orevactaene pigments produced by Epicoccum nigrum in solid-state fermentation
  126. Treatment of denture stomatitis using iron nanoparticles green-synthesized by Silybum marianum extract
  127. Characterization and antioxidant potential of white mustard (Brassica hirta) leaf extract and stabilization of sunflower oil
  128. Characteristics of Langmuir monomolecular monolayers formed by the novel oil blends
  129. Strategies for optimizing the single GdSrFeO4 phase synthesis
  130. Oleic acid and linoleic acid nanosomes boost immunity and provoke cell death via the upregulation of beta-defensin-4 at genetic and epigenetic levels
  131. Unraveling the therapeutic potential of Bombax ceiba roots: A comprehensive study of chemical composition, heavy metal content, antibacterial activity, and in silico analysis
  132. Green synthesis of AgNPs using plant extract and investigation of its anti-human colorectal cancer application
  133. The adsorption of naproxen on adsorbents obtained from pepper stalk extract by green synthesis
  134. Treatment of gastric cancer by silver nanoparticles encapsulated by chitosan polymers mediated by Pistacia atlantica extract under ultrasound condition
  135. In vitro protective and anti-inflammatory effects of Capparis spinosa and its flavonoids profile
  136. Wear and corrosion behavior of TiC and WC coatings deposited on high-speed steels by electro-spark deposition
  137. Therapeutic effects of green-formulated gold nanoparticles by Origanum majorana on spinal cord injury in rats
  138. Melanin antibacterial activity of two new strains, SN1 and SN2, of Exophiala phaeomuriformis against five human pathogens
  139. Evaluation of the analgesic and anesthetic properties of silver nanoparticles supported over biodegradable acacia gum-modified magnetic nanoparticles
  140. Review Articles
  141. Role and mechanism of fruit waste polyphenols in diabetes management
  142. A comprehensive review of non-alkaloidal metabolites from the subfamily Amaryllidoideae (Amaryllidaceae)
  143. Discovery of the chemical constituents, structural characteristics, and pharmacological functions of Chinese caterpillar fungus
  144. Eco-friendly green approach of nickel oxide nanoparticles for biomedical applications
  145. Advances in the pharmaceutical research of curcumin for oral administration
  146. Rapid Communication
  147. Determination of the contents of bioactive compounds in St. John’s wort (Hypericum perforatum): Comparison of commercial and wild samples
  148. Retraction
  149. Retraction of “Two mixed-ligand coordination polymers based on 2,5-thiophenedicarboxylic acid and flexible N-donor ligands: The protective effect on periodontitis via reducing the release of IL-1β and TNF-α”
  150. Topical Issue on Phytochemicals, biological and toxicological analysis of aromatic medicinal plants
  151. Anti-plasmodial potential of selected medicinal plants and a compound Atropine isolated from Eucalyptus obliqua
  152. Anthocyanin extract from black rice attenuates chronic inflammation in DSS-induced colitis mouse model by modulating the gut microbiota
  153. Evaluation of antibiofilm and cytotoxicity effect of Rumex vesicarius methanol extract
  154. Chemical compositions of Litsea umbellata and inhibition activities
  155. Green synthesis, characterization of silver nanoparticles using Rhynchosia capitata leaf extract and their biological activities
  156. GC-MS analysis and antibacterial activities of some plants belonging to the genus Euphorbia on selected bacterial isolates
  157. The abrogative effect of propolis on acrylamide-induced toxicity in male albino rats: Histological study
  158. A phytoconstituent 6-aminoflavone ameliorates lipopolysaccharide-induced oxidative stress mediated synapse and memory dysfunction via p-Akt/NF-kB pathway in albino mice
  159. Anti-diabetic potentials of Sorbaria tomentosa Lindl. Rehder: Phytochemistry (GC-MS analysis), α-amylase, α-glucosidase inhibitory, in vivo hypoglycemic, and biochemical analysis
  160. Assessment of cytotoxic and apoptotic activities of the Cassia angustifolia aqueous extract against SW480 colon cancer
  161. Biochemical analysis, antioxidant, and antibacterial efficacy of the bee propolis extract (Hymenoptera: Apis mellifera) against Staphylococcus aureus-induced infection in BALB/c mice: In vitro and in vivo study
  162. Assessment of essential elements and heavy metals in Saudi Arabian rice samples underwent various processing methods
  163. Two new compounds from leaves of Capparis dongvanensis (Sy, B. H. Quang & D. V. Hai) and inhibition activities
  164. Hydroxyquinoline sulfanilamide ameliorates STZ-induced hyperglycemia-mediated amyleoid beta burden and memory impairment in adult mice
  165. An automated reading of semi-quantitative hemagglutination results in microplates: Micro-assay for plant lectins
  166. Inductively coupled plasma mass spectrometry assessment of essential and toxic trace elements in traditional spices consumed by the population of the Middle Eastern region in their recipes
  167. Phytochemical analysis and anticancer activity of the Pithecellobium dulce seed extract in colorectal cancer cells
  168. Impact of climatic disturbances on the chemical compositions and metabolites of Salvia officinalis
  169. Physicochemical characterization, antioxidant and antifungal activities of essential oils of Urginea maritima and Allium sativum
  170. Phytochemical analysis and antifungal efficiency of Origanum majorana extracts against some phytopathogenic fungi causing tomato damping-off diseases
  171. Special Issue on 4th IC3PE
  172. Graphene quantum dots: A comprehensive overview
  173. Studies on the intercalation of calcium–aluminium layered double hydroxide-MCPA and its controlled release mechanism as a potential green herbicide
  174. Synergetic effect of adsorption and photocatalysis by zinc ferrite-anchored graphitic carbon nitride nanosheet for the removal of ciprofloxacin under visible light irradiation
  175. Exploring anticancer activity of the Indonesian guava leaf (Psidium guajava L.) fraction on various human cancer cell lines in an in vitro cell-based approach
  176. The comparison of gold extraction methods from the rock using thiourea and thiosulfate
  177. Special Issue on Marine environmental sciences and significance of the multidisciplinary approaches
  178. Sorption of alkylphenols and estrogens on microplastics in marine conditions
  179. Cytotoxic ketosteroids from the Red Sea soft coral Dendronephthya sp.
  180. Antibacterial and biofilm prevention metabolites from Acanthophora spicifera
  181. Characteristics, source, and health risk assessment of aerosol polyaromatic hydrocarbons in the rural and urban regions of western Saudi Arabia
  182. Special Issue on Advanced Nanomaterials for Energy, Environmental and Biological Applications - Part II
  183. Green synthesis, characterization, and evaluation of antibacterial activities of cobalt nanoparticles produced by marine fungal species Periconia prolifica
  184. Combustion-mediated sol–gel preparation of cobalt-doped ZnO nanohybrids for the degradation of acid red and antibacterial performance
  185. Perinatal supplementation with selenium nanoparticles modified with ascorbic acid improves hepatotoxicity in rat gestational diabetes
  186. Evaluation and chemical characterization of bioactive secondary metabolites from endophytic fungi associated with the ethnomedicinal plant Bergenia ciliata
  187. Enhancing photovoltaic efficiency with SQI-Br and SQI-I sensitizers: A comparative analysis
  188. Nanostructured p-PbS/p-CuO sulfide/oxide bilayer heterojunction as a promising photoelectrode for hydrogen gas generation
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