Startseite Optimization of the biological synthesis of silver nanoparticles using Penicillium oxalicum GRS-1 and their antimicrobial effects against common food-borne pathogens
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Optimization of the biological synthesis of silver nanoparticles using Penicillium oxalicum GRS-1 and their antimicrobial effects against common food-borne pathogens

  • Gaurav Kumar Rose , Raman Soni , Praveen Rishi und Sanjeev Kumar Soni EMAIL logo
Veröffentlicht/Copyright: 31. August 2018
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Green Processing and Synthesis
Aus der Zeitschrift Green Processing and Synthesis Band 8 Heft 1

Abstract

Biologically synthesized nanoparticles are gaining importance as they offer several advantages, such as the ease with which they can be scaled up, the cost-effectiveness of the process and the green route of production. In this study, silver (Ag) nanoparticles were biosynthesized using the cellular extract of Penicillium oxalicum GRS-1 and then characterized by ultraviolet visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. The biosynthesis of nanoparticles was optimized by following the one factor at a time approach, wherein the temperature of 60°C, pH 7.0 and 1.5 mm silver nitrate (AgNO3) concentration were found to be most favorable factors for the production of Ag nanoparticles. Upon statistical optimization, the maximum production of Ag nanoparticles with a concentration of 136 ppm was achieved at pH 7.2, AgNO3 concentration 1.975 mm and 86 h using the crude cellular extract of P. oxalicum GRS-1 having nitrate reductase activity. TEM analysis showed that the Ag nanoparticles were spherical in shape with sizes ranging from 10 to 40 nm. The biosynthesized nanoparticles showed strong antimicrobial activity against the common food-borne, pathogens including Staphylococcus aureus, Escherichia coli and Salmonella typhimurium with respective minimum bactericidal concentrations of 32, 16 and 32 μg/ml.

Keywords: antimicrobial; optimization; Penicillium oxalicum; silver nanoparticles

1 Introduction

Nanotechnology involves the control of matter at the sub-atomic nanoscale level [1], wherein the properties of matter are altogether different from their plainly visible mass properties [2]. Nanoparticles are the fundamental building blocks for producing numerous nanostructured materials and gadgets. [3]. Nanoparticle production is a vital part of the quickly thriving research endeavors in the fields of nanoscience and nanoengineering [2]. There is a growing urgency of employing environment-friendly technology for nanoparticle production that does not produce toxic wastes. To achieve this, we are inclined to adopt economical production processes, which appear to be of biological origin [2]. Nanoparticles synthesized by a biological approach are more desirable, in copious ways, to those synthesized by chemical methods [4], [5] and has several advantages, including easy scaling up of the procedure, cost-effectiveness and its green nature.

Numerous biological agents, including bacteria, yeasts, fungi and plant extracts, play an essential role in remediation of toxic metals through metal ion reduction. These agents can act as eco-friendly nanofactories for the biosynthesis of cadmium, gold and silver nanoparticles [6], [7], [8], [9]. Fungi appear to be a good candidate for the biosynthesis of metal nanoparticles because of the presence of a range of bio-catalysts and reducing agents in their cells. Fungi secrete large amounts of proteins compared with bacteria, which results in the biosynthesis of large amounts of nanoparticles. Of the various nanoparticles, Ag nanoparticles have attracted the greatest attention because of their wide range of applications for the welfare of the society [10]. In all the life forms that can biosynthesize Ag nanoparticles, nitrate reductase may be an indispensable part. The reduction mediated by the presence of this enzyme in the organism has been observed to be responsible for the biosynthesis of nanoparticles [11], [12], [13], [14].

Ag nanoparticles have emerged as a principal product from the field of nanobiotechnology. Due to the expanding microbial resistance against antibiotics and the evolution of resistant strains, the biosynthesis and use of Ag nanoparticles is an important field of interest for many researchers [15], [16]. The high efficiency of Ag nanoparticles is mainly due to the scope of a larger surface area to volume ratio for interactions, which facilitate the attachment of Ag nanoparticles to the cell membrane and also penetration inside the bacteria [17], [18], [19]. Ag nanoparticles interact with the sulfur, which contains membrane proteins of the bacteria, as well as with the phosphorous, which contains compounds like DNA. The nanoparticles preferably attack the respiratory chain, cell division and finally lead to cell death. The Ag ions are released by the nanoparticles inside the bacterial cells, which intensify their bactericidal activity [20]. Aside from its major use as an antimicrobial agent, Ag nanoparticles are recognized to have the toxic effects in many different species. Therefore, substantial test trials are required to understand the toxicity of such nanoparticles. In the present study, the crude cellular extract from Penicillium oxalicum GRS-1 was successfully employed for the biosynthesis of Ag nanoparticles. Results indicated its inhibitory activity against common food-borne pathogens.

2 Materials and methods

2.1 Microorganism

The fungal strain of P. oxalicum GRS-1, used in the present study, was isolated from soil after an extensive screening of natural biodiversity of Chandigarh City and identified on the basis of microscopic and molecular analysis of 18S rDNA sequencing.

2.2 Production of nitrate reductase by P. oxalicum GRS-1 and the biosynthesis of Ag nanoparticles

Fungal biomass was produced by inoculation with spore suspension (1.7×107 spores/ml) from a 4-day old culture of P. oxalicum GRS-1 in 50 ml MGYP broth (malt extract, 0.3%; glucose 1%, yeast extract, 0.3%; peptone, 0.5%; pH 5.0) (Hi-Media, Mumbai, India) dispensed in 250 ml Erlenmeyer flasks and incubated on a rotary shaker (Bio-age, India) (150 rpm, 28°C) for 96 h. Fungal biomass was filtered and washed properly with distilled water and 10 g of the same was dispensed in 100 ml of distilled water taken in 250 ml Erlenmeyer flask, incubated on a rotary shaker (150 rpm, 28°C) for 48 h. The cell filtrate was obtained by the separation of fungal beads through the whatman filter paper no. 1 and analyzed for nitrate reductase activity. This was then mixed with AgNO3 solution at a final concentration of 1 mm incubated on a rotary shaker (150 rpm, 28°C) for 72 h and cell filtrate without AgNO3 was used as a control [21]. The transformations of the Ag ions into Ag nanoparticles were monitored by the UV-visible spectrophotometer and the nanoparticles were collected by ultracentrifugation (Hitachi Himac, CS 120GX, Japan) at 100,000 g for 30 min, purified by giving washings in alcohol and stored for further characterization.

2.2.1 Nitrate reductase assay in the cell-free supernatant

The nitrate reductase assay was performed by using the procedure as laid down by Gilliam et al. [22]. The enzyme activity was expressed in terms of International Units (IU) equivalent to the nanomoles KNO2 produced from KNO3 per hour under assay conditions (40°C, pH 7.5).

2.3 Characterization of the Ag nanoparticles

The nanoparticles were characterized by X-ray Diffraction (XRD) (Panalytical.s X.Pert Pro, Malvern Panalytical, Malvern, UK), field emission scanning electron microscopy (FESEM) (Model JSM6100, Jeol, Peabody, MA, USA), transmission electron microscopy (TEM) (Hitachi, H-7500, Japan) and Fourier transform infrared spectroscopy (FTIR) (Model RZX, Perkin Elmer, Billerica, MA, USA) [23] at the sophisticated analytical instrumentation facility, Panjab University, Chandigarh.

2.4 Optimization of the biological synthesis of the Ag nanoparticles

There are several factors that affect the biosynthesis, shape and size of metal nanoparticles. The effect of various factors like pH and temperature, substrate (AgNO3) concentration and biomass concentration on the biosynthesis of Ag nanoparticles was studied by using one variable at a time approach.

2.5 Statistical optimization of the Ag nanoparticle production by P. oxalicum GRS-1 using response surface methodology (RSM)

The effects of changes in various environmental parameters and their interactions were evaluated by employing a statistical modeling with RSM. RSM uses a statistical approach to assess the effect of changes in the conditions of different environmental factors on Ag nanoparticle production in order to find out the optimum conditions for their production. Three factors, including pH (X1), AgNO3 concentration (X2) and incubation time (X3), which affect nanoparticle production was chosen for further standardizing the Ag nanoparticle biosynthesis by Penicillium oxalicum GRS-1. A 23 factorial central composite trial configuration bringing about 20 trial runs were produced by Design Expert, Version 10.0 (Stat-Ease Inc. Minneapolis, MN, USA). The connection amongst coded and genuine values is depicted by Equation (1)

(1)Xi=(Xi−X0i)/δXi,I=1, 2, 3……..j

where Xi=coded (dimensionless) estimation of variable Xi, which is the actual estimation of the ith variable; X0=the value of Xi at the center point and δX=is the step change in value. The conduct of the framework was clarified by the following second order polynomial equation:

(2)Y=bo+Σbi xi+ΣΣbij xi xj+Σbii x2 i+e,

where Y=measured reaction; bo, bi, bij and bii are the constant and regression coefficients of the model; xi and xj are the levels (code values) of independent values and e is random error. The Design Expert, Version 10.0 (Stat-Ease Inc. Minneapolis, MN, USA) was utilized for the regression analysis of the information obtained and to measure the coefficients of the regression equation. 3D surface graphs were designed by using the Design expert software to analyze the relationship between the factors. The accuracy of the general ability of the polynomial model was assessed by the coefficient of determination (R2). The statistical significance of the model coefficient was evaluated by one-way analysis of variance (ANOVA). The response in all the 20 flasks was expressed in terms of the concentration (ppm) of the biosynthesized Ag nanoparticles.

2.6 In vitro assessment of the anti-bacterial activity of the Ag nanoparticles

The inhibitory impact of the Ag nanoparticles against the common food-borne pathogens, including Staphylococcus aureus, Escherichia coli and Salmonella typhimurium, was evaluated by agar well diffusion test [24]. The cultures were grown separately overnight in 50 ml nutrient broth (NB) dispensed in 150 ml Erlenmeyer flasks and incubated as shake cultures at 37°C. Next, 100 μl of each actively grown culture with cell count of approximately 106 cfu/ml was spread over the nutrient agar plates, in which three wells (7 mm) were punched for the addition of fungal filtrate and Ag nanoparticle solution. The Ag nanoparticle solutions (50 and 100 μg/ml) were placed in their respective wells and the plates were incubated for 24 h at 37°C, and the antibacterial effectiveness was assessed on the basis of the zones of inhibition around the wells.

2.7 Determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the biologically synthesized Ag nanoparticles against common food borne pathogens

The MIC of Ag nanoparticles was determined by using the broth micro dilution method against the common food-borne pathogens, including S. aureus, E. coli and S. typhimurium. About 2 ml of the geometric concentrations of nanoparticles (32, 64, 128, 256, 512, 1024, 2048 μg/ml) in Mueller Hinton broth (MHB) were used in test tubes (12×100 mm) and then inoculated separately with each of actively grown cultures with a cell count of approximately 106 cfu/ml. The positive control consisted of inoculated MHB broth and the negative control contained only uninoculated MHB medium. The incubation time and temperature were taken 24 h and 37°C. The MIC was determined as the lowest concentration of Ag nanoparticles, which repressed the noticeable development of bacteria after overnight incubation. The MBC was determined by plating the substance of the tubes containing MIC and a higher concentration of biosynthesized Ag nanoparticles on nutrient agar plate and incubated at 37°C for 24 h. MBC was defined as the lowest concentration of Ag nanoparticles resulting in the >99.9% reduction of the initial inoculums. All analyses were carried out in triplicates.

2.8 Data analysis

All the values are expressed as mean±standard deviation of three independent experiments with three replicates each. The results were statistically analyzed using ANOVA with the Holm-Šídák method using a sigma plot (Systat Software, San Jose, CA, USA).

3 Results

3.1 Morphological and molecular analysis of the strain GRS-1

The microscopic examination of the fungal strain isolated from the natural biodiversity showed smooth mycelium with branched conidiophores having ellipsoidal conidiospores and smooth walls in a long chain, which are the typical characteristics of the genus Penicillium [25]. On the basis of the nucleotide homology and phylogenetic analysis of the 18S rDNA sequence, the strain GRS-1 was found to be close to P. oxalicum BT (Gen Bank Accession Number: KT189509.1) and hence was named as P. oxalicum GRS-1.

3.2 Production of the nitrate reductase by P. oxalicum GRS-1 and the biosynthesis of Ag nanoparticles

The reduction of Ag+ to Ag0 was mediated by the bio-molecules present in the cell filtrate of P. oxalicum GRS-1, which revealed the nitrate reductase activity of 94.61 IU/ml. The biosynthesis of Ag nanoparticles was visually recognized by the appearance of brown color in the solution containing cell filtrate and AgNO3. The presence of Ag nanoparticles was confirmed by the ultraviolet (UV)-visible spectra of the cell filtrate with AgNO3, which demonstrated a strong wide peak between 410 and 450 nm (surface plasmon resonance band). The power of the SPR band continuously increased from 4 to 96 h as a function of time of response.

3.3 Characterization of the Ag nanoparticles

3.3.1 X-Ray diffraction analysis

XRD analysis demonstrated the development of Ag nanoparticles, which was affirmed by various diffraction crests in the XRD investigation, as indicated in Figure 1. Bragg’s reflection was clearly depicted and distinctly exhibited in the XRD pattern of the biosynthesized Ag nanoparticles, the peaks were recorded on the basis of face-centered cubic (FCC). The XRD graph exhibited sharp peaks at 9, 11, 21, 22, 27, 32, 38 and 46 positions relating to the diffractions from the planes of Ag with FCC cross sections. The patterns absolutely showed that Ag nanoparticles were synthesized by the reduction of metal ions by P. oxalicum GRS-1. The sharpening of peaks clearly demonstrated that the particles were in nanoregime. The average size of the nanocrystalites, as estimated from FWHM using the Scherrer’s formula, D=0.94 λ/β cosθ, was in the range of 19–36 nm.

Figure 1: The XRD analysis of the biologically synthesized Ag nanoparticles.
Figure 1:

The XRD analysis of the biologically synthesized Ag nanoparticles.

3.3.2 FESEM analysis

The shape of the biosynthesized Ag nanoparticles as determined by FESEM micrographs was spherical and monodispersed (Figure 2A–E). The elemental profile of the biologically synthesized Ag nanoparticles, as analyzed by EDX graphs, showed the optical absorption peak at 3 KeV, which corresponds to metallic Ag and hence confirms the biosynthesis of the Ag nanoparticles (Figure 3).

Figure 2: The FESEM images of the biologically synthesized Ag nanoparticles using cellular extract of Penicillium oxalicum GRS-1. (A) Ag nanoparticles attached with fungal mycelium at 1.0 μm, (B) Ag nanoparticles in cluster at 500 nm, (C) Ag nanoparticles in cluster at 400 nm, (D) Separated Ag nanoparticles at 500 nm, (E) Ag nanoparticles at 1.0 μm and (F) Ag nanoparticles at 300 nm.
Figure 2:

The FESEM images of the biologically synthesized Ag nanoparticles using cellular extract of Penicillium oxalicum GRS-1. (A) Ag nanoparticles attached with fungal mycelium at 1.0 μm, (B) Ag nanoparticles in cluster at 500 nm, (C) Ag nanoparticles in cluster at 400 nm, (D) Separated Ag nanoparticles at 500 nm, (E) Ag nanoparticles at 1.0 μm and (F) Ag nanoparticles at 300 nm.

Figure 3: The EDX spectra of the biologically synthesized Ag nanoparticles using cellular extract of Penicillium oxalicum GRS-1.
Figure 3:

The EDX spectra of the biologically synthesized Ag nanoparticles using cellular extract of Penicillium oxalicum GRS-1.

3.3.3 TEM analysis

Nanoparticles of different sizes running from 10 to 100 nm (Figure 4) were observed as separated and aggregated in TEM analysis. The morphology of the nanoparticles was highly variable and they were not in coordinate contact even within the aggregates, thus demonstrating the stabilization of the nanoparticles by a capping agent.

Figure 4: The TEM images of the Ag nanoparticles synthesized using the cellular extract of Penicillium oxalicum GRS-1. (A) TEM image of Ag nanoparticles at a scale of 100 nm. (B) Selected area electron diffraction pattern (SAED) of Ag nanoparticles. (C) High Resolution TEM image of biologically synthesized Ag nanoparticles.
Figure 4:

The TEM images of the Ag nanoparticles synthesized using the cellular extract of Penicillium oxalicum GRS-1. (A) TEM image of Ag nanoparticles at a scale of 100 nm. (B) Selected area electron diffraction pattern (SAED) of Ag nanoparticles. (C) High Resolution TEM image of biologically synthesized Ag nanoparticles.

3.3.4 FTIR spectroscopy analysis

The FTIR analysis of the biologically synthesized Ag nanoparticles revealed peaks corresponding to the N-H stretch at 3638.59, 3699.59 and 3787.59 cm−1, alkyl C-H stretch at 2854.54 cm−1 and 2924.52 cm−1 as well as the C=O stretch of ester at 1742.54 cm−1. The vibrational bands at 1551.54 and 1464 cm−1 corresponded to the N-H stretch and C-N (amines) asymmetric stretch in the amide linkages of the proteins, respectively (Figure 5).

Figure 5: The FTIR spectrum of the Ag nanoparticles synthesized using the cell-free extracts of Penicillium oxalicum GRS-1.
Figure 5:

The FTIR spectrum of the Ag nanoparticles synthesized using the cell-free extracts of Penicillium oxalicum GRS-1.

3.4 Optimization of the biological synthesis of Ag nanoparticles

3.4.1 Effect of pH

pH is one of the major environmental factor that influences the production of Ag nanoparticles. The Ag nanoparticls production was very slow in an acidic range where smaller and broader peaks were observed. With the increase in pH of the solution, the absorbance peaks were found to be narrower and sharper, indicating the spherical and smaller sized production of Ag nanoparticles. At pH 7.0 and 8.0 the peak was found to be highly symmetrical, thus confirming the uniform distribution of nanoparticles (Figure 6A). The maximum peak intensity corresponding to 1.333 was observed at 450 nm at pH 7.0, indicating the higher concentration of Ag nanoparticles produced in the solution (Table 1).

Figure 6: The effects of pH (A), temperature (B), AgNO3 concentration (C) and fungal biomass concentration (D) on the biosynthesis of Ag nanoparticles using the cellular extract from Penicillium oxalicum GRS-1.
Figure 6:

The effects of pH (A), temperature (B), AgNO3 concentration (C) and fungal biomass concentration (D) on the biosynthesis of Ag nanoparticles using the cellular extract from Penicillium oxalicum GRS-1.

Table 1:

The effects of various parameters on the peak intensity of the biosynthesized Ag nanoparticles.

pHPeak intensityTemperature (°C)Peak intensityAgNO3 concentration (mm)Peak intensityBiomass concentration (g)Peak intensity
50.229±0.0501a,b,c200.275±0.0322b0.51.236±0.11850.12±0.0425
60.888±0.0872b,e,I,l,m300.52±0.0432b1.02.089±0.3b,d,f100.847±0.0843
71.333±0.578d,e,f,g401.847±0.171a1.52.149±0.26a,c,f151.102±0.108a
81.003±0.0887g,h,I,k502.146±0.132a2.01.84±0.097a,b,e201.288±0.198a
90.936±0.044a,f,j,k,l603.063±0.2222.51.922±0.095c,d,e251.979±0.0388
100.854±0.0832c,d,h,j,m

  1. a–mValues with the same letter in the same column do not differ significantly based on the Holm-Šídák test (p>0.001). All the other values differ significantly from each one another with p<0.001.

3.4.2 Effect of temperature

Temperature is one of the key controlling factor affecting the formation of Ag nanoparticles. With an increase in temperature from 20°C to 60°C, there was shifting of peak towards a lower wavelength, which revealed a reduction in particle size (Figure 6B). Broader peaks were observed at a low temperature, which indicated the existence of different sizes of Ag nanoparticles. In comparison, the narrower peaks were obtained at a higher temperature, which showed the presence of a narrow range of Ag nanoparticles in the solution. The highest and narrower peak was obtained at 60°C. The peak intensity was found to be maximum (3.063) at 418 nm, indicating the presence of a large number of Ag nanoparticles in the solution (Table 1). The peak was found to be most symmetrical at 40°C, which indicated that the biosynthesized Ag nanoparticles were monodispersed in nature.

3.4.3 Effect of the AgNo3 concentration

The influence of AgNo3 concentration on the production of Ag nanoparticles was evaluated by varying the concentration of AgNo3 (Figure 6C). There was a gradual increase in the production of Ag nanoparticles by up to 1.5 mm AgNO3 concentration. The maximum value of peak intensity corresponding to 2.149 in the presence of 1.5 mm AgNO3 was found to be at 414 nm, which indicated the higher production of Ag nanoparticles (Table 1). With an increase in the AgNO3 concentration, there was distortion in the peak symmetry and the peak became flattened, thus indicating non-uniformity in particle size.

3.4.4 Effect of the fungal biomass concentration

With an increase in biomass concentration from 5 to 25 g, more symmetrical, sharp and narrower absorbance peaks were obtained, indicating the small sized and uniform distribution of Ag nanoparticles (Figure 6D). The maximum value of peak intensity corresponding to 1.979 was found to be at 414 nm with 25 g biomass (Table 1), thus indicating the highest production of Ag nanoparticles.

3.5 Statistical optimization of Ag nanoparticle production by response surface methodology (RSM)

The experimental designs resulting in 20 experimental runs with the examined responses for Ag nanoparticles generation are presented in Table 2. The regression equation was created to investigate the interacting factors by recognizing the critical factors adding to the regression model and determining the optimal values of the most noteworthy independent factors. To choose the right sufficiency of model for Ag nanoparticles production, two different tests viz., sequential model sum of squares and model summary statistics were carried out. Different models, including linear, 2F1, quadratic and cubic, were employed to try to improve the Ag nanoparticles biosynthesis on the premise of their F-value, p-value, determination coefficient (R2), standard deviation and PRESS values. Of the considerable number of models examined, the quadratic regression model was observed to be noteworthy. The ANOVA analysis indicated a linear relationship between the significant effects of pH, AgNo3 concentration and incubation time; the interactions between pH and AgNo3 concentration, between AgNo3 and incubation time and between pH and incubation time; the quadratic relationship with pH; AgNo3 concentration and incubation time. By applying multiple regression analysis on the trial data, the following second-order polynomial equation was found to clarify the Ag nanoparticle biosynthesis by just considering the noteworthy terms and is shown in Equation (3).

Table 2:

The central composite design matrix with the experimental values of the Ag nanoparticles produced with Penicillium oxalicum GRS-1.

RunspHAgNO3 concentration (mm)Incubation time (h)Ag nanoparticles (ppm)
17.001.5072.00136
27.001.5072.00139
38.002.0096.00131
46.001.0048.0069
58.001.0048.0074
67.001.50120.00119
77.001.5072.00138
87.000.5072.0055
97.001.5072.00135
108.002.0048.00125
116.002.0048.0078
129.001.5072.0079
136.002.0096.00107
148.001.0096.0090
156.001.0096.00111
167.001.5072.00138
177.002.5072.0090
187.001.5024.0065
197.001.5072.00137
205.001.5072.0057
(3)Ag nanoparticle yield=138.20+6.19×A+10.44×B+ 12.56×C+10.88×A×B−6.12×A×C−2.87×B×C− 16.77×A2−15.65×B2−10.77×C2,

where A, B and C are pH, AgNo3 concentration and incubation time, respectively. The ANOVA for the Ag nanoparticle biosynthesis, as depicted in Table 3, demonstrates the model F-value of 89.30, showing that the model is significant. There is just a 0.01% possibility that a “Model F-Value” this extensive could happen due to noise. The p-values less than 0.0500 showed the significance of the model terms. The estimation of correlation coefficient (R2=0.9877) demonstrated that 98.7% inconstancy can be revealed by the model. The value signal to noise proportion (S/N), which is a measure of the satisfactory accuracy is 25.090. A value greater than 4 is desirable in support of the fitness of the model. The coefficient of variation (CV) indicates the degree of precision with which the treatments are compared. Usually, the larger the value of CV, the lower the dependability of the experiment is. In this experiment, a lower value of CV corresponding to 4.54 indicated a greater reliability of the analysis performed. The thus investigation demonstrated that the form of the model chosen to reveal the relationship between the factors and the response was correct. In this case A, B, C, AB, AC, A2, B2 and C2 are significant model terms. However, some regression coefficients (i.e. BC) were found to be unnecessary, having p-values>0.05, thus suggesting their insignificance. However, this was still included in order to improve the model.

Table 3:

The ANOVA results for the response surface quadratic model employed for the biosynthesis of the Ag nanoparticles.

SourceSum of squaresCoefficient estimatet-testF valuep-Value
Model17789.21138.204573.5189.30<0.0001Significant
A-pH612.566.18755.2427.680.0004
B-AgNO31743.0610.43758.84578.75<0.0001
C-incubation time2525.0612.562510.646114.08<0.0001
AB946.1310.8756.5542.75<0.0001
AC300.13−6.125−3.68913.560.0042
BC66.13−2.875−1.732.990.1146
A27073.30−16.7727−17.84319.57<0.0001
B26156.26−15.6477−16.64278.14<0.0001
C22917.87−10.7727−11.46131.83<0.0001
Residual221.34138.2045

3.5.1 Interactions among the factors

Student’s t-test was used to analyze the knowledge of the error mean square that is crucial in examining the significance of the predicted coefficient of the regression equation. The greater the magnitude of the t value and the smaller the p value, the more significant is the corresponding coefficient. The coefficient estimates and t values in the quadratic model, as depicted in Table 3 revealed that factors A, B and C had direct effects on Ag nanoparticle production with incubation time, revealing the maximal effect. The inter communication between the factors AC and BC revealed the opposing effects on nanoparticle production, whereas the interactions between AB had positive effects on Ag nanoparticle production. The 3D surface graphs (Figure 7) revealed the interconnection between the two factors for the optimization of conditions for the Ag nanoparticle production. From the plots, we can easily see the interactions between two factors and locate the optimum levels. Each curve exhibits an immense number of combinations of two test variables with the other variables sustained at the constant level. The surface graph, which was accomplished as a function of pH versus AgNo3 concentration, showed that Ag nanoparticle production was enhanced with the increase of both pH and AgNo3; however, at high concentration, the nanoparticle production decreased. The maximum production of Ag nanoparticles corresponding to 141.563 ppm was obtained in the optimized medium, where the pH and AgNo3 concentration were 7.33 and 1.72 mm, respectively (Figure 7A) while incubation time was held at the 0 code level. The surface graph obtained as a function of pH of reaction mixture versus incubation time showed that the nanoparticle production increased with the increase in pH and incubation time. The maximum production of Ag nanoparticles corresponding to 141.974 ppm occurred at a pH 7.09 and 85.50 h of incubation time while the AgNo3 concentration was held at the 0 code level (Figure 7B). Figure 7C shows the effect of the AgNo3 concentration and incubation time on the Ag nanoparticle production. As can be seen, an increase in the concentration of AgNo3 and duration of incubation time promoted the Ag nanoparticle production. However, the Ag nanoparticle production decreased at a high concentration of AgNo3 (1.64 mm) and incubation time duration of 85.04 h. The maximum production of the Ag nanoparticles corresponding to 143.108 ppm was obtained at 1.64 mm of AgNo3 and the incubation time of 85.04 h while the pH value was held at the 0 code level.

Figure 7: The 3D surface graphs representing the Ag nanoparticle yields from the crude cellular extracts of Penicillium oxalicum GRS-1 as affected by pH and AgNO3 concentration (A), pH and incubation time (B), incubation time and AgNO3 concentration (C) keeping the other variables at 0 coded values.
Figure 7:

The 3D surface graphs representing the Ag nanoparticle yields from the crude cellular extracts of Penicillium oxalicum GRS-1 as affected by pH and AgNO3 concentration (A), pH and incubation time (B), incubation time and AgNO3 concentration (C) keeping the other variables at 0 coded values.

3.5.2 Model validation

In order to determine the precision of the statistical experimental model of RSM, efforts were made to develop the process parameters of reaction mixture for augmenting the Ag nanoparticle production using different variables. The numerical optimization for Ag nanoparticle production was attempted with Design expert using A (pH, 7.2), B (AgNo3 concentration, 1.975 mm) and C (incubation time, 85.775 h), incubated at 40°C in shaking condition at 150 rpm. This procedure predicted the yield corresponding to 139.069 ppm. To substantiate the optimum concentrations, an experiment with the above particularized conditions was carried out and the result corresponded to 136 ppm, which was found to be very close to the anticipated value. This high degree of precision affirmed the reliability of the model with only an insignificant error due to the negligible deflection in experimental conditions. The statistical interpretation of environmental conditions thus intensifies the production of Ag nanoparticles to a considerable amount.

3.6 In vitro assessment of the anti-bacterial activity of the Ag nanoparticles

The biologically synthesized Ag nanoparticles at a concentration of 100 μg/ml showed very strong inhibitory effect against common food-borne pathogens, including S. aureus, E. coli and S. typhimurium revealing 14, 15 and 14 mm zones of inhibition, respectively (Table 4).

Table 4:

The inhibitory effect of the Ag nanoparticles against various pathogens achieved though the well diffusion test method.

PathogenZone of inhibition (fungal cell filtrate)Zone of inhibition (50 μg/ml of AgNPs)Zone of inhibition (100 μg/ml of AgNPs)
Staphylococcus aureus–10 mm14 mm
Salmonella typhimurium–10 mm14 mm
Escherichia coli–12 mm15 mm

3.6.1 Determination of the MIC and MBC of the biologically synthesized Ag nanoparticles

After 24 h of incubation at 37°C, turbidity was seen in the test tubes containing 8 and 16 μg/ml Ag nanoparticles, indicating the growth of S. aureus and S. typhimurium, while in the case of E. coli, turbidity was observed in the test tube containing 8 μg/ml of Ag nanoparticles only. Further, no turbidity was observed in the test tubes containing 32–256 μg/ml of Ag nanoparticles in the cases of S. aureus and S. typhimurium and 16–256 μg/ml of Ag nanoparticles in the case of E. coli, thus indicating the inhibition of bacterial growth.

To confirm the growth, the suspension from all the test tubes, including negative and positive controls, was inoculated in nutrient agar plates and incubated for 24 h. Bacterial growth was observed in the area marked positive control, 8 and 16 μg/ml of Ag nanoparticles in case of S. aureus and S. typhimurium, whereas in the case of E. coli, bacterial growth was observed in the area marked positive control and 8 μg/ml of Ag nanoparticles. Further, no growth was observed in the area marked 32–256 μg/ml of Ag nanoparticles in the cases of S. aureus and S. typhimurium and 16–256 μg/ml of Ag nanoparticles in the case of E. coli. The MBC of Ag nanoparticles against S. aureus, and S. typhimurium was found to be 32 μg/ml and that for E. coli was 16 μg/ml, indicating that Ag nanoparticles have both bacteriostatic and bactericidal activities (Table 5).

Table 5:

The minimum bactericidal concentration of the Ag nanoparticles after 24 h on agar plates.

PathogenNegative controlPositive controlAgNOPs (μg/ml)
08163264128256
Salmonella typhimurium–+++––––
Staphylococcus aureus–+++––––
Escherichia coli–++–––––

4 Discussion

Nanoparticles play a significant role in various fields, including environment, health care, energy, agriculture, and so on. Large numbers of microbes have been found to be capable of biosynthesizing nanoparticles as their cellular extracts act as both reducing as well as capping agents. The nitrate reductase-mediated biosynthesis of Ag nanoparticles is the most acknowledged process, and during this catalysis, nitrate is reduced to nitrite and an electron is transported to the approaching Ag ions. The direct evidence was reported by Anil Kumar et al. [14], who straightforwardly utilized the purified nitrate reductase from the Fusarium oxysporum for the production of the Ag nanoparticles. In the present report, a natural isolate of P. oxalicum GRS-1 was utilized for the production of Ag nanoparticles owing to the presence of nitrate reductase in its cellular extracts. The reddish brown shade of the solution was due to the SPR of the Ag nanoparticles formed [26]. These results are similar to the findings of Wiley et al. [27], who revealed that the aggregate oscillation of conduction electrons inside metal particles facilitated the diffusion and assimilation of light at a specific frequency, giving them shading on account of silver and gold. The UV-Vis spectra of the cell filtrate with AgNO3 demonstrated a solid wide crest between 420 and 460 nm (SPR band), which were particular for Ag nanoparticles. These results are consistent with the reports of Verma et al. [28], who revealed that the Ag nanoparticles can be biosynthesized extracellularly using Aspergillus clavatus as the fungal system.

The characterization of the Ag nanoparticles was done by using different techniques like XRD analysis, FTIR, SEM and TEM. The development and nature of the biosynthesized nanoparticles were checked by XRD patterns [29]. The XRD graphs of the biosynthesized Ag nanoparticles exhibited sharp peaks at the 9, 11, 21, 22, 27, 32, 38 and 46 positions corresponding to the diffraction from the planes of Ag with the FCC lattice. The patterns indicated that the Ag nanoparticles were formed through the reduction of metal ions by cellular extract obtained from P. oxalicum GRS-1. The sharpening of peaks clearly indicate that the particles are in nanorange.

FESEM analysis revealed that the biosynthesized Ag nanoparticles are even shaped and spherical in nature. These results strongly confirm the idea that the cellular extract obtained from P. oxalicum GRS-1 might act as a reducing and capping agents in the biosynthesis of Ag nanoparticles [30]. The reduction of Ag ions into Ag nanoparticles is additionally confirmed by EDX analysis, which revealed an optical absorption peak at 3 Kev corresponding to metallic Ag [31].

Meanwhile, TEM results revealed further details about the morphology and size of the biologically synthesized Ag nanoparticles and the same were found to be in the size range of 10–40 nm. Further, the TEM analysis of the biosynthesized Ag nanoparticles confirmed the presence of capping proteins around the Ag nanoparticles [32]. The absorption peak obtained at 280 nm confirmed the presence of aromatic amino acids (tyrosine and tryptophan) residues of proteins, which act as capping agent around the Ag nanoparticles. The Ag nanoparticles were crystalline in nature, as can be seen from the chosen region diffraction design recorded from one of the nanoparticles in the cluster [31].

FTIR was employed for the detection of possible functional groups within the capping proteins associated with the nanoparticles, which may be responsible for the reduction and stabilization of the Ag nanoparticles. The appearance of peaks at 1551.54 and 1464 cm−1 represented the amide 1 and amide 3 bands in the FTIR spectra which revealed the presence of protein around the Ag nanoparticles. The vibrational bands at 1551.54 and 1464 cm−1 corresponding to the N-H stretch and C-N (amines) asymmetric stretch, respectively, were also found in the amide linkages of the proteins (Figure 5). These functional groups play important roles in maintaining stability and capping of Ag nanoparticles, as reported in many studies [30], [33]. Amide 1 and amide 3 spectral bands have been found to be most sensitive to the variation in the secondary structure folding. Overall, the results affirm that the capping protein stabilizes the metallic nanoparticle and inhibits the agglomeration in the medium. This investigation confirms that the production and stabilization of the Ag nanoparticles in the aqueous medium can be achieved by utilizing biological molecules [31].

The biosynthesis of the Ag nanoparticles by cellular extracts obtained from the mycelia of P. oxalicum GRS-1 was optimized by standardizing the effects of pH, temperature, AgNO3 concentration and fungal biomass concentration. In case of the effect of pH study, there was no nanoparticle formation in the acidic range but there was gradual increase in nanoparticle production with an increase in pH. The production of nanoparticles in the alkaline region shows that the enzyme nitrate reductase was active under the alkaline environment [34]. At pH 8.0, the peak was found to be most symmetrical, indicating the uniform distribution of nanoparticles. At pH 7.0 and 8.0, the nanoparticle production was most symmetrical. Furthermore, nanoparticle production peak became increasingly symmetrical with an increase in pH, which can be explained by the fact that the OH− groups plays a crucial role in the stabilization of Ag nanoparticles and that more nucleation regions are formed due to the availability of OH−, which can prevent the aggregation and assist in maintaining the symmetry with small-sized Ag nanoparticles [35], [36].

With an increase in temperature of the nanoparticle biosynthesis, there was a shifting of peak towards the lower wavelength, which means that the increase in temperature resulted in reduced particle size [37]. This can be probably explained by the fact that, with the increase in temperature, there was also an increase in nucleation, which occurred at various places. The increase in temperature also affects the rate at which the nanoparticles were produced. In this report, the value of peak intensity (3.115) was found to be maximum at 418 nm, thus demonstrating that the yield of the Ag nanoparticles in the current study is higher than that in previous reports. The presence of a band at above 260 nm was attributed to the electronic excitation in tryptophan and the tyrosine residues in protein, which indicated the presence of protein molecules in colloidal solution [38].

In terms of the influence of the AgNo3 concentration, the maximum biosynthesis of Ag nanoparticles was achieved at the AgNO3 concentration of 1.5 mm. At lower concentrations of AgNO3, the availability of substrate for the enzyme was insufficient, which showed a decrease in the production of Ag nanoparticles. At higher concentrations of AgNO3, the peak symmetry was disturbed, and the peak becomes flattened, indicating that the particles of non-uniform sizes were formed. Such results are in accordance with that of Anil Kumar et al. [14], who revealed the effect of AgNO3 concentration on the biosynthesis of the Ag nanoparticles in E. coli.

One of the important factors responsible for the biosynthesis of the Ag nanoparticles is organism’s enzymes. In the present study, we used the crude cellular extracts obtained from fungal biomass for the biosynthesis of Ag nanoparticles due to the ease in downstream processing of nanoparticles. With increase in biomass concentration (5–25 g), more symmetrical, sharper and narrower absorbance peaks were obtained, indicating the presence of the spherical, small-sized and uniform distribution of the Ag nanoparticles [39], [40]. Further, the highest peak intensity corresponding to 1.979 was found to be at 414 nm with 25 g biomass, indicating the highest production of Ag nanoparticles. The pattern of increased Ag nanoparticle production with rise in biomass concentration may be attributed to the release of more nitrate reductase from mycelium in the supernatant in the latter (data not shown).

The process of P. oxalicum GRS-1 mediating the biological synthesis of the Ag nanoparticles was influenced by various environmental factors, as observed in the study, which employed the one variable at a time approach. The effects of changes in various environmental conditions, including pH, temperature and incubation time, were also evaluated by employing statistical modeling by RSM, which is an empirical modeling technique that is used to evaluate the relationship between a set of controllable experimental factors and observed results [41]. A second-order model like central composite design (CCD) is widely used in RSM because it can take on a wide variety of functional forms, and such flexibility allows it to predict the true response surface more closely. This approach has already been successfully employed to maximize the productivities of various enzymes [42], [43], [44], [45], [46], [47], [48]. The major environmental factors that affect the production of Ag nanoparticles are pH and temperature. The main goal of RSM is to optimize the various environmental conditions to bring about the increased production of the metal nanoparticles. According to our report, the maximum Ag nanoparticle production amounting to 136 ppm was achieved when the crude cellular extracts of P. oxalicum GRS-1 were incubated at pH 7.2, AgNo3 concentration of 1.975 mm and incubation time of 85.775 h. This report demonstrated a higher production of Ag nanoparticles than the previously reported results [11], [13], [23], [30].

The antimicrobial efficacy of the biosynthesized Ag nanoparticles in terms of MIC and MBC has been reported in many studies [49], [50], [51], [52], [53]. The nanoparticles preferably attack the respiratory chain, cell division and finally lead to cell death. The Ag nanoparticles interact with the sulfur containing membrane proteins of the bacteria as well as with the phosphorous containing compounds like DNA. The antimicrobial action of the Ag nanoparticles on gram negative bacteria was based on the concentration of Ag nanoparticles and was closely associated with the arrangement of pits in the cell wall of bacteria [54]. Ag has a solid antimicrobial potential, which has been used since the ancient times. However, with the advancement in the field of antibiotics, the therapeutic applications of Ag as antimicrobial were reduced [55], [56]. The antimicrobial impact of Ag can be expanded by controlling their size at the nano level. Due to the rapid progress in the optimization process, Ag nanoparticles have emerged as potential antimicrobial agents due to their high surface area to volume ratio and unique physiochemical properties [20]. Ag nanoparticles with sizes ranging from 10 to 100 nm have shown very effective antimicrobial behaviors against both gram-positive and gram-negative bacteria [57], [58]. Ag nanoparticles biologically synthesized from P. oxalicum GRS-1 have also been found to be highly effective against common food-borne pathogens, including S. aureus, E. coli and S. typhimurium with MBC values of 32, 16 and 32 μg/ml, respectively.

5 Conclusion

Green synthesis provides an eco-friendly, cost-effective and efficient approach for the production of Ag nanoparticles, which could act as excellent antimicrobial agents against pathogenic microbes. The present study has isolated a strain of P. oxalicum GRS-1 from soil, which showed capability of reducing Ag ions into Ag nanoparticles extracellularly with sizes ranging from 10 to 40 nm. The yield of Ag nanoparticles, as evident from the peak intensity, was found to be higher than the previous reports, thus imparting novelty in the study. The biosynthesized Ag nanoparticles were found to be effective against common food-borne pathogens, including S. aureus, E. coli and S. typhimurium with MBC values of 32, 16 and 32 μg/ml, respectively. Thus, the strain is a potential candidate for developing a green technology for the commercial biosynthesis of Ag nanoparticles. The utilization of such biosynthesized nanoparticles in bactericidal applications makes this approach possibly impressive for the large-scale biosynthesis of nanomaterials.

Acknowledgments

The financial assistance provided by the Indian Council of Medical Research (ICMR), India in the form of a Research Fellowship to Mr. Gaurav Kumar is highly acknowledged.

  1. Conflict of interest statement: The authors declare that they have no conflict of interest.

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Received: 2018-02-06
Accepted: 2018-07-10
Published Online: 2018-08-31
Published in Print: 2019-01-28

©2019 Walter de Gruyter GmbH, Berlin/Boston

This work is licensed under the Creative Commons Attribution 4.0 Public License.

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  79. Improved removal of methylene blue on modified hierarchical zeolite Y: Achieved by a “destructive-constructive” method
  80. Two different facile and efficient approaches for the synthesis of various N-arylacetamides via N-acetylation of arylamines and straightforward one-pot reductive acetylation of nitroarenes promoted by recyclable CuFe2O4 nanoparticles in water
  81. Optimization of acid catalyzed esterification and mixed metal oxide catalyzed transesterification for biodiesel production from Moringa oleifera oil
  82. Kinetics and the fluidity of the stearic acid esters with different carbon backbones
  83. Aiming for a standardized protocol for preparing a process green synthesis report and for ranking multiple synthesis plans to a common target product
  84. Microstructure and luminescence of VO2 (B) nanoparticle synthesis by hydrothermal method
  85. Optimization of uranium removal from uranium plant wastewater by response surface methodology (RSM)
  86. Microwave drying of nickel-containing residue: dielectric properties, kinetics, and energy aspects
  87. Simple and convenient two step synthesis of 5-bromo-2,3-dimethoxy-6-methyl-1,4-benzoquinone
  88. Biodiesel production from waste cooking oil
  89. The effect of activation temperature on structure and properties of blue coke-based activated carbon by CO2 activation
  90. Optimization of reaction parameters for the green synthesis of zero valent iron nanoparticles using pine tree needles
  91. Microwave-assisted protocol for squalene isolation and conversion from oil-deodoriser distillates
  92. Denitrification performance of rare earth tailings-based catalysts
  93. Facile synthesis of silver nanoparticles using Averrhoa bilimbi L and Plum extracts and investigation on the synergistic bioactivity using in vitro models
  94. Green production of AgNPs and their phytostimulatory impact
  95. Photocatalytic activity of Ag/Ni bi-metallic nanoparticles on textile dye removal
  96. Topical Issue: Green Process Engineering / Guest Editors: Martine Poux, Patrick Cognet
  97. Modelling and optimisation of oxidative desulphurisation of tyre-derived oil via central composite design approach
  98. CO2 sequestration by carbonation of olivine: a new process for optimal separation of the solids produced
  99. Organic carbonates synthesis improved by pervaporation for CO2 utilisation
  100. Production of starch nanoparticles through solvent-antisolvent precipitation in a spinning disc reactor
  101. A kinetic study of Zn halide/TBAB-catalysed fixation of CO2 with styrene oxide in propylene carbonate
  102. Topical on Green Process Engineering
https://doi.org/10.1515/gps-2018-0042
Schlagwörter für diesen Artikel
antimicrobial; optimization; Penicillium oxalicum; silver nanoparticles
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BY 4.0

Artikel in diesem Heft

  1. Regular Articles
  2. Studies on the preparation and properties of biodegradable polyester from soybean oil
  3. Flow-mode biodiesel production from palm oil using a pressurized microwave reactor
  4. Reduction of free fatty acids in waste oil for biodiesel production by glycerolysis: investigation and optimization of process parameters
  5. Saccharin: a cheap and mild acidic agent for the synthesis of azo dyes via telescoped dediazotization
  6. Optimization of lipase-catalyzed synthesis of polyethylene glycol stearate in a solvent-free system
  7. Green synthesis of iron oxide nanoparticles using Platanus orientalis leaf extract for antifungal activity
  8. Ultrasound assisted chemical activation of peanut husk for copper removal
  9. Room temperature silanization of Fe3O4 for the preparation of phenyl functionalized magnetic adsorbent for dispersive solid phase extraction for the extraction of phthalates in water
  10. Evaluation of the saponin green extraction from Ziziphus spina-christi leaves using hydrothermal, microwave and Bain-Marie water bath heating methods
  11. Oxidation of dibenzothiophene using the heterogeneous catalyst of tungsten-based carbon nanotubes
  12. Calcined sodium silicate as an efficient and benign heterogeneous catalyst for the transesterification of natural lecithin to L-α-glycerophosphocholine
  13. Synergistic effect between CO2 and H2O2 on ethylbenzene oxidation catalyzed by carbon supported heteropolyanion catalysts
  14. Hydrocyanation of 2-arylmethyleneindan-1,3-diones using potassium hexacyanoferrate(II) as a nontoxic cyanating agent
  15. Green synthesis of hydratropic aldehyde from α-methylstyrene catalyzed by Al2O3-supported metal phthalocyanines
  16. Environmentally benign chemical recycling of polycarbonate wastes: comparison of micro- and nano-TiO2 solid support efficiencies
  17. Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange
  18. Production of value-added chemicals from esterification of waste glycerol over MCM-41 supported catalysts
  19. Green synthesis of zerovalent copper nanoparticles for efficient reduction of toxic azo dyes congo red and methyl orange
  20. Optimization of the biological synthesis of silver nanoparticles using Penicillium oxalicum GRS-1 and their antimicrobial effects against common food-borne pathogens
  21. Optimization of submerged fermentation conditions to overproduce bioethanol using two industrial and traditional Saccharomyces cerevisiae strains
  22. Extraction of In3+ and Fe3+ from sulfate solutions by using a 3D-printed “Y”-shaped microreactor
  23. Foliar-mediated Ag:ZnO nanophotocatalysts: green synthesis, characterization, pollutants degradation, and in vitro biocidal activity
  24. Green cyclic acetals production by glycerol etherification reaction with benzaldehyde using cationic acidic resin
  25. Biosynthesis, characterization and antimicrobial activities assessment of fabricated selenium nanoparticles using Pelargonium zonale leaf extract
  26. Synthesis of high surface area magnesia by using walnut shell as a template
  27. Controllable biosynthesis of silver nanoparticles using actinobacterial strains
  28. Green vegetation: a promising source of color dyes
  29. Mechano-chemical synthesis of ammonia and acetic acid from inorganic materials in water
  30. Green synthesis and structural characterization of novel N1-substituted 3,4-dihydropyrimidin-2(1H)-ones
  31. Biodiesel production from cotton oil using heterogeneous CaO catalysts from eggshells prepared at different calcination temperatures
  32. Regeneration of spent mercury catalyst for the treatment of dye wastewater by the microwave and ultrasonic spray-assisted method
  33. Green synthesis of the innovative super paramagnetic nanoparticles from the leaves extract of Fraxinus chinensis Roxb and their application for the decolourisation of toxic dyes
  34. Biogenic ZnO nanoparticles: a study of blueshift of optical band gap and photocatalytic degradation of reactive yellow 186 dye under direct sunlight
  35. Leached compounds from the extracts of pomegranate peel, green coconut shell, and karuvelam wood for the removal of hexavalent chromium
  36. Enhancement of molecular weight reduction of natural rubber in triphasic CO2/toluene/H2O systems with hydrogen peroxide for preparation of biobased polyurethanes
  37. An efficient green synthesis of novel 1H-imidazo[1,2-a]imidazole-3-amine and imidazo[2,1-c][1,2,4]triazole-5-amine derivatives via Strecker reaction under controlled microwave heating
  38. Evaluation of three different green fabrication methods for the synthesis of crystalline ZnO nanoparticles using Pelargonium zonale leaf extract
  39. A highly efficient and multifunctional biomass supporting Ag, Ni, and Cu nanoparticles through wetness impregnation for environmental remediation
  40. Simple one-pot green method for large-scale production of mesalamine, an anti-inflammatory agent
  41. Relationships between step and cumulative PMI and E-factors: implications on estimating material efficiency with respect to charting synthesis optimization strategies
  42. A comparative sorption study of Cr3+ and Cr6+ using mango peels: kinetic, equilibrium and thermodynamic
  43. Effects of acid hydrolysis waste liquid recycle on preparation of microcrystalline cellulose
  44. Use of deep eutectic solvents as catalyst: A mini-review
  45. Microwave-assisted synthesis of pyrrolidinone derivatives using 1,1’-butylenebis(3-sulfo-3H-imidazol-1-ium) chloride in ethylene glycol
  46. Green and eco-friendly synthesis of Co3O4 and Ag-Co3O4: Characterization and photo-catalytic activity
  47. Adsorption optimized of the coal-based material and application for cyanide wastewater treatment
  48. Aloe vera leaf extract mediated green synthesis of selenium nanoparticles and assessment of their In vitro antimicrobial activity against spoilage fungi and pathogenic bacteria strains
  49. Waste phenolic resin derived activated carbon by microwave-assisted KOH activation and application to dye wastewater treatment
  50. Direct ethanol production from cellulose by consortium of Trichoderma reesei and Candida molischiana
  51. Agricultural waste biomass-assisted nanostructures: Synthesis and application
  52. Biodiesel production from rubber seed oil using calcium oxide derived from eggshell as catalyst – optimization and modeling studies
  53. Study of fabrication of fully aqueous solution processed SnS quantum dot-sensitized solar cell
  54. Assessment of aqueous extract of Gypsophila aretioides for inhibitory effects on calcium carbonate formation
  55. An environmentally friendly acylation reaction of 2-methylnaphthalene in solvent-free condition in a micro-channel reactor
  56. Aegle marmelos phytochemical stabilized synthesis and characterization of ZnO nanoparticles and their role against agriculture and food pathogen
  57. A reactive coupling process for co-production of solketal and biodiesel
  58. Optimization of the asymmetric synthesis of (S)-1-phenylethanol using Ispir bean as whole-cell biocatalyst
  59. Synthesis of pyrazolopyridine and pyrazoloquinoline derivatives by one-pot, three-component reactions of arylglyoxals, 3-methyl-1-aryl-1H-pyrazol-5-amines and cyclic 1,3-dicarbonyl compounds in the presence of tetrapropylammonium bromide
  60. Preconcentration of morphine in urine sample using a green and solvent-free microextraction method
  61. Extraction of glycyrrhizic acid by aqueous two-phase system formed by PEG and two environmentally friendly organic acid salts - sodium citrate and sodium tartrate
  62. Green synthesis of copper oxide nanoparticles using Juglans regia leaf extract and assessment of their physico-chemical and biological properties
  63. Deep eutectic solvents (DESs) as powerful and recyclable catalysts and solvents for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones/thiones
  64. Biosynthesis, characterization and anti-microbial activity of silver nanoparticle based gel hand wash
  65. Efficient and selective microwave-assisted O-methylation of phenolic compounds using tetramethylammonium hydroxide (TMAH)
  66. Anticoagulant, thrombolytic and antibacterial activities of Euphorbia acruensis latex-mediated bioengineered silver nanoparticles
  67. Volcanic ash as reusable catalyst in the green synthesis of 3H-1,5-benzodiazepines
  68. Green synthesis, anionic polymerization of 1,4-bis(methacryloyl)piperazine using Algerian clay as catalyst
  69. Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production
  70. Biosynthetic potential assessment of four food pathogenic bacteria in hydrothermally silver nanoparticles fabrication
  71. Investigating the effectiveness of classical and eco-friendly approaches for synthesis of dialdehydes from organic dihalides
  72. Pyrolysis of palm oil using zeolite catalyst and characterization of the boil-oil
  73. Azadirachta indica leaves extract assisted green synthesis of Ag-TiO2 for degradation of Methylene blue and Rhodamine B dyes in aqueous medium
  74. Synthesis of vitamin E succinate catalyzed by nano-SiO2 immobilized DMAP derivative in mixed solvent system
  75. Extraction of phytosterols from melon (Cucumis melo) seeds by supercritical CO2 as a clean technology
  76. Production of uronic acids by hydrothermolysis of pectin as a model substance for plant biomass waste
  77. Biofabrication of highly pure copper oxide nanoparticles using wheat seed extract and their catalytic activity: A mechanistic approach
  78. Intelligent modeling and optimization of emulsion aggregation method for producing green printing ink
  79. Improved removal of methylene blue on modified hierarchical zeolite Y: Achieved by a “destructive-constructive” method
  80. Two different facile and efficient approaches for the synthesis of various N-arylacetamides via N-acetylation of arylamines and straightforward one-pot reductive acetylation of nitroarenes promoted by recyclable CuFe2O4 nanoparticles in water
  81. Optimization of acid catalyzed esterification and mixed metal oxide catalyzed transesterification for biodiesel production from Moringa oleifera oil
  82. Kinetics and the fluidity of the stearic acid esters with different carbon backbones
  83. Aiming for a standardized protocol for preparing a process green synthesis report and for ranking multiple synthesis plans to a common target product
  84. Microstructure and luminescence of VO2 (B) nanoparticle synthesis by hydrothermal method
  85. Optimization of uranium removal from uranium plant wastewater by response surface methodology (RSM)
  86. Microwave drying of nickel-containing residue: dielectric properties, kinetics, and energy aspects
  87. Simple and convenient two step synthesis of 5-bromo-2,3-dimethoxy-6-methyl-1,4-benzoquinone
  88. Biodiesel production from waste cooking oil
  89. The effect of activation temperature on structure and properties of blue coke-based activated carbon by CO2 activation
  90. Optimization of reaction parameters for the green synthesis of zero valent iron nanoparticles using pine tree needles
  91. Microwave-assisted protocol for squalene isolation and conversion from oil-deodoriser distillates
  92. Denitrification performance of rare earth tailings-based catalysts
  93. Facile synthesis of silver nanoparticles using Averrhoa bilimbi L and Plum extracts and investigation on the synergistic bioactivity using in vitro models
  94. Green production of AgNPs and their phytostimulatory impact
  95. Photocatalytic activity of Ag/Ni bi-metallic nanoparticles on textile dye removal
  96. Topical Issue: Green Process Engineering / Guest Editors: Martine Poux, Patrick Cognet
  97. Modelling and optimisation of oxidative desulphurisation of tyre-derived oil via central composite design approach
  98. CO2 sequestration by carbonation of olivine: a new process for optimal separation of the solids produced
  99. Organic carbonates synthesis improved by pervaporation for CO2 utilisation
  100. Production of starch nanoparticles through solvent-antisolvent precipitation in a spinning disc reactor
  101. A kinetic study of Zn halide/TBAB-catalysed fixation of CO2 with styrene oxide in propylene carbonate
  102. Topical on Green Process Engineering
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