Startseite A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis
Artikel Open Access

A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis

  • Pinzhu Qin , Dawei Huang EMAIL logo , Zihao Xu , Ying Guan , Yongxin Bing EMAIL logo und Ang Yu
Veröffentlicht/Copyright: 31. Dezember 2019
Artikel downloaden (PDF)
Open Chemistry
Aus der Zeitschrift Open Chemistry Band 17 Heft 1

Abstract

An aptasensor for the detection of ochratoxin A (OTA) in environmental samples was developed. It displayed high sensitivity and good selectivity. Factors such as specific binding between a FAM (5-carboxyfluorescein)-labeled aptamer (f-RP) and OTA, and a magnetic property of a streptavidin magbeads-modified capture probe (bm-CP) resulted in aptasensor’s linear relationship between fluorescence intensity and the concentration of OTA. This characteristic is present at the OTA concentration ranges from 0.100 μM to 25.00 μM with a LOD (limit of detection) of 0.0690 μM. The bm-CP can be reused through melting, washing and magnetic separation, which contributes to cost reduction. In addition, the proposed method is simple and detection process is fast. The aptasensor can be used in real samples.

Keywords: ochratoxin A; aptamer; fluorescence; magnetic particles; reused

1 Introduction

Environmental pollution and food safety have always been the focus of attention around the world [1, 2]. Mycotoxins including ochratoxin A (OTA), aflatoxin B1, aflatoxin B2, fumonisin B1 and zearalenone are toxicity secondary metabolites produced by fungi or molds [3]. Among them, OTA is widely distributed in nature and has extremely high toxicity. Humans and animals are exposed to OTA in variety of ways, for example through cereals, spices, beans, soluble coffee, dried fruit, grape juice, milk, and honey. OTA poses a great threat to human health, because it can cause toxic kidney damage, hepatotoxicity, immunosuppression or lead to cancer, teratogenicity, and mutagenesis [4, 5]. Considering the results of these severe toxic effects, several countries regulate the maximum levels of OTA. In Switzerland, the permissible amount of OTA in compound feeds for pigs and poultry should not exceed 200 μg/kg and 1000 μg/kg [6].

Traditional methods for OTA analysis include thin layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC), liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-fluorescence detection (HPLC-FLD), capillary electrophoresis (CE), and immunoassay [7, 8, 9, 10, 11, 12]. Although chromatographic analysis is sensitive, it is limited by the complexity of the operation, the expensive equipment and the need for professionals to operate in practical condition. Therefore, it cannot meet the needs of an on-site rapid detection [13,14]. The methods used for on-site rapid detection are mainly immunoassay methods, including colloidal gold fast type (direct method), colloidal gold precision type (extracted reconstitution method), and Elisa kit. However, a method based on the immunological technique is costly and is easily affected by environmental factors [15, 16, 17]. Hence, a simple, low-cost and fast detection method is desirable. Fortunately, with the aptamer-specific, OTA was screened out and reported, and a number of sensors have been established using aptamers.

Aptamer is an oligonucleotide fragment which can be screened in vitro (systematic evolution of ligands by exponential enrichment, SELEX) and can bind to the corresponding ligands through various interactions such as Van der Waals forces, hydrogen bonding, electrostatic interactions, and shape matching [18, 19, 20, 21, 22]. More importantly, aptamer possess several advantages such as thermal stability, reusability, is easy to synthesize, with more sensitivity than antigen reaction [23]. Thus, aptamer-based strategies have been widely applied in food safety analysis, environmental pollution analysis and clinical diagnosis [24,25].

Fluorescence labeling is an attractive analytical method due to its stability and high detection sensitivity. Researchers developed different types of sensors such as immunolabeling molecule beacon fluorescent biosensor and biosensor fluorescence sensor with an aptamer structure switch [26, 27, 28]. Recently, the fluorescence method that involved magnetic nanoparticles has attracted the attention of researchers. Jiang et al. described the application and development of nanomaterial-enabled biosensors in OTA detection in the past years (2007-2018) [29]. Magnetic nanoparticles are widely used in the field of molecular biology because of their good biocompatibility, superparamagnetism, low toxicity and easy preparation [30], where the viruses are separated and the environment purified [31,32]. An oligonucleotide modified by magnetic nanoparticle can be prepared as capture probe, while a fluorescent labeling aptamer can be prepared as a recognition probe. After being hybridized and separated, the capture probe can be regenerated through thermal treatment for next experiment, which can reduce cost. As far as we know, fluorescence analysis methods based on magnetic nanoparticles have not been reported to detect OTA in the environment.

In this work, a fluorescent analysis method based on magnetic nanoparticles is developed to detect OTA. The OTA aptamer recognition probe was labeled with fluorescent dyes (f-RP), and the capture probe was labeled with biotin (b-CP) and then modified with magnetic nanoparticles (bm-CP). f-RP was added to the test sample to specifically bind with OTA, and bm-CP was added to hybridize with the unreacted f-RP. The fluorescence signal of the supernatant was measured after magnetic separation. Furthermore, the bm-CP was suggested to be reused after magnetic separation, cross-linking, washing, and re-magnetic separation.

2 Experimental

2.1 Reagents and chemicals

OTA was purchased from Sigma Corporation, aflatoxin B1 (AFB1) and SSC buffer powder were obtained from Yuanye Biological Technology Co., Ltd, streptavidin magbeads (10mg/mL, 100nm) were purchased from Aladdin Industrial Corporation. (Hydroxymethyl)-aminomethane (Tris), tween, sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4) and disodium phosphate (Na2HPO4) were obtained from Sinopharm Chemical Reagent Co., Ltd (Guangdong, China). Ultrapure water was used throughout the experiment (18.3 MΩ cm). The ssDNA sequences used in the experiment are as follows: aptamer (f-RP): 5’-FAM-GAT CGG GTG TGG GTG GCG TAA AGG GAG CAT CGG ACA-3’, capture probe (b-CP): 5’-biotin-TTT TTT CCG ATG CTC CCT-3’. The aptamer and capture probe were purchased from Sangon Biotech (Shanghai, China). The aptamer stock solution obtained by dissolving the oligonucleotides in 260 μL of PBS, the capture probe was dissolved in 580 μL of PBS, the two chains were stored at 4oC before usage.

2.2 Preparation of buffer solution and OTA solution

Tris binding buffer solution containing tween (TBBT): 50.0 mL of 0.10 M tris-hydroxymethane solution (0.6056 g of tris was added to 50.0 mL water) and 42.0 mL of 0.10 M hydrochloric acid (9.0 mL of concentrated hydrochloric acid was added to 1000 mL water) were diluted it to 1.0 L.

PBS buffer solution: 0.27g of KH2PO4, 1.42g of Na2HPO4, 8.00g of NaCl and 0.20g of KCl were added to 800 mL water, and then concentrated hydrochloric acid was added to adjust pH to 7.4, finally dilute it to 1.0 L.

OTA solution: 5.00 mg of OTA was added to 5.00 mL methanol and forming a 1.00 g/L standard solution. 1009 μL 1.00 g/L standard solution was shifted to 100.0 mL volumetric flask and then a 25.0 μM OTA reserve solution was obtained.

2.3 Preparation of bm-CP

48.0 µL of 10.0 mg/mL streptavidin magbeads and 8.0 μL of 25.0 μM b-CP were added to the reaction flask, the reaction was conducted at 37oC for 30 min, finally the bm-CP was obtained.

2.4 Apparatus

All fluorescence measurements were carried out on a F4600 fluorometer (Hitachi, Japan). Other instruments used during the experiment included high-speed (low-speed) centrifuges, constant-temperature digital water baths, oscillators, magnets, ultrasonic cleaners, sterilizers, and drying ovens.

2.5 The detection process for OTA and the recovery of bm-CP

After 80.0 μL of TBBT and 8.0 μL of 25.0 μM f-RP were added to the reaction flask, 8.0 μL of 25.0 μM OTA solution was added, the mixed solution was incubated at 45oC for 20 min. Then, 56.0 μL of bm-CP was added and hybridized with unreact f-RP at 30oC for 50 min; Next, the reaction flask was placed on a magnetic rack for magnetic separation, the supernatant was removed and then rinsed by TBBT. Finally, the fluorescence intensity of supernatant was measured (λex=488 nm, λem=518 nm). After the measurement, 300.0 μL of TBBT was added to the reaction flask containing magnetic nanoparticle complexes at the bottom, and the solution was shaken at a constant temperature shaker in 45oC for 30 min. After that, the supernatant was removed and the magnetic nanoparticles complex bm-CP were recovered from the bottom of the flask.

Ethical approval: The conducted research is not related to either human or animal use.

3 Results and Discussion

3.1 Experimental principle

The principle of the bioassay for OTA detection is illustrated in Figure 1. The establishment of this experiment was based on: a) the specific binding of aptamer and OTA, b) streptavidin magbeads attraction by magnets. The aptamer of OTA was labeled with FAM (f-RP), the capture probe (designed by the OTA aptamer) labeled with biotin and modified with streptavidin magbeads (bm-CP). After f-RP was combined with OTA, the bm-CP was added to hybridize with unreacted f-RP. Given enough time for hybridization, the bm-CP at the bottom of the reactor was retrieved by a magnet and the supernatant was then transferred to the cuvette and subjected to the process of magnetic separation. Therefore, the amount of OTA can be determined by measuring the fluorescence intensity of the supernatant. Three sample repeats were prepared in the experiments and all results were averaged from the three parallel experiments. In addition, the bm-CP could be recycled after melting, washing and re-magnetic separation, which saved cost.

Figure 1 The experimental principal of the bioassay for OTA detection.
Figure 1

The experimental principal of the bioassay for OTA detection.

3.2 Optimization of the aptasensor

In order to save the streptavidin magbeads and obtain ideal experiment results, the optimization of the ratio between b-CP and streptavidin magbeads was necessary. Four optimization molar ratios were tested (b-CP: streptavidin magbeads) = 1:2, 1:3, 1:4, and 1:5 with the same amounts of OTA and f-RP. As seen from Figure 2, when the ratio was 1:4, the fluorescence intensity was the weakest, indicating sufficient bm-CP and the best combination of b-CP and streptavidin magbeads. To facilitate the experiments, the ratio of 1:4 was chosen as the optimal ratio in the sensing system.

Figure 2 Effects of the ratio between b-CP and streptavidin magbeads.
Figure 2

Effects of the ratio between b-CP and streptavidin magbeads.

The different types of buffer solutions will affect the final result of the detection system [33]. Three different types of buffer solutions (tris, TBBT and SSC) were compared to determine the most suitable buffer in this bio-system. The results (Figure 3) show that the fluorescence intensity measured in the tris buffer solution was significantly higher than that measured in the SSC buffer solution, indicating that the tris buffer solution should be selected compared to SSC buffer solution. The effects of adding TBBT to tris buffer solution or not (tris) were investigated and their impact on the experiments were analyzed . The results show that TBBT can effectively enhance the fluorescence intensity. All the results indicate that TBBT is the best choice as the buffer solution.

Figure 3 Effects of different buffer solutions.
Figure 3

Effects of different buffer solutions.

Lower hybridization temperature will result in insufficient hybridization and higher temperature will cause dissolution of the oligonucleotide chain [34,35]. Temperatures such as 25, 30, 35, 40oC were selected to for testing. The results are shown in Figure 4. It was found that the highest fluorescent intensity was obtained at 30oC after 30 min of hybridization. Hybridization time was detected and it was found that the fluorescence intensity increased along with the time and reached the highest point at 50 min (the mean fluorescence intensity was found to be 565.22±19.80 (n=3), 635.57±9.19 (n=3), 716.55±12.73 (n=3), and 880.50±23.33 (n=3) for the hybridization time of 20 min, 30 min, 40 min, and 50 min, respectively). Therefore, 30oC was chosen as the optimization hybridized temperature and 50 min as the optimization hybridized time.

Figure 4 Effects of hybridized temperature.
Figure 4

Effects of hybridized temperature.

Furthermore, in order to realize the recovery of bm-CP, it is necessary to select the best temperature and time for derotation between f-RP and bm-CP. The results revealed that 45oC for 30 min was the optimum conditions (Figure 5 and Figure 6). After melting, the bm-CP was washed and magnetic particles separated. The results show that the recycled bm-CP have almost no effect on the experimental data due to its excessive amount in the proposed method.

Figure 5 Effects of derotation temperature.
Figure 5

Effects of derotation temperature.

Figure 6 Effects of derotation time.
Figure 6

Effects of derotation time.

3.3 The sensitivity of the aptasensor

Under optimized conditions, the sensitivity of the aptasensor is revealed in Figure 7. Different concentrations of OTA were selected to measure the change of fluorescent intensity. The various concentrations of OTA were 0, 0.0010, 0.010, 0.100, 1.00, 10.0, and 25.0 μM. The results indicated that higher OTA concentration results in greater fluorescence intensity (Figure 7a). Corresponding to the amplified curves in Figure 7b, a good linear relationship from 0.10 μM to 25.0 μM was obtained and the limit of detection found as 0.0690 μM (calculated by 3S+F¯−bK,where S is the standard deviation of black control, K is the slope of the regression equation, F¯is the average background fluorescence intensity, b is the intercept in the linear regression equation).

Figure 7 Sensitivity of this aptasensor: (a) Plot of fluorescence intensity dependent on OTA concentrations; (b) OTA and its fluorescence intensity linear curve and linear regression equation.
Figure 7

Sensitivity of this aptasensor: (a) Plot of fluorescence intensity dependent on OTA concentrations; (b) OTA and its fluorescence intensity linear curve and linear regression equation.

3.4 The selectivity of the aptasensor

The experimental principle is based on the specific binding of the aptamer and OTA and it is necessary to define if the OTB, whose structure is similar to OTA, will interfere with the aptasensor (OTB is a dechlorination derivative of OTA). As seen from Figure 8, it is clear that fluorescent signal of OTA is much stronger than that of OTB. These phenomena might be attributed to the strong specificity and high affinity between aptamer and target, their combined stable structures such as G-tetrad and fake, the hydrogen bonding and Van der Waals forces [36, 37, 38]. This result revealed that our aptasensor was very selective for OTA detection.

Figure 8 Selectivity of our aptasensor.
Figure 8

Selectivity of our aptasensor.

3.5 Preliminary determination of OTA in real samples

In real life, a lot of OTA can be produced after the mildew from corn, rice, and bread [39]. In order to determine whether the aptasensor can be effectively applied in actual samples, an applicability test was conducted. First, 1.0 mg of moldy corn, rice, and bread material was added to a mixed solution (5.0 mL of alcohol and 5.0 mL of water), then the established aptasensor was used to determine the amount of OTA in the three samples. As shown in Figure 9, the fluorescence intensity of the system is significantly higher than the blank sample, indicating that this method can be applied to the detection of OTA in actual samples.

Figure 9 The practical application of this aptasensor.
Figure 9

The practical application of this aptasensor.

4 Conclusions

The aptasensor takes the advantage of the magnetic nanoparticles properties and it can be moved in a magnetic field. The unreacted system can be separated from the reaction system rapidly by using a magnet after a completed hybridization. As a result, a sensitive and selective method was develped with a simple and fast detection process. The streptavidin magbeads-modified capture probe (bm-CP) can be recovered by thermal analysis, achieving the goal of reusability at low cost. In addition, the process of preparing bm-CP again was eliminated, making the detection quicker and simpler. The detection limit of this method can reach nM level. Furthermore, there were no large instruments applied in the reaction steps, making the aptasensor easier more practical.

Acknowledgements

This work was financially supported by the Natural Science Foundation of Guangdong Province, China (2016A030310022), the National Natural Science Foundation of China (21507034), the Natural Science Fund for Colleges and Universities in Jiangsu Province (16KJB610003), and the Opening Foundation of Jiangsu Province Key Laboratory of Environmental Engineering (KF2014008).

  1. Conflict of interest: Authors state no conflict of interest.

References

[1] Li F., Zhang J.D., Liu W.C., Liu J.A., Huang J.H., Zeng G.M., An exploration of an integrated stochastic-fuzzy pollution assessment for heavy metals in urban topsoil based on metal enrichment and bioaccessibility. Science of The Total Environment, 2018, 644(10), 649-660.10.1016/j.scitotenv.2018.06.366Suche in Google Scholar PubMed

[2] Wang H., Li F, Dong Y.M., Li Z.J., Wang G.L., Ferricyanide stimulated cathodic photoelectrochemistry of flower-like bismuth oxyiodide under ambient air: A general strategy for robust bioanalysis, Sensors and Actuators B: Chemical, 2019, 288(1), 683-690.10.1016/j.snb.2019.03.066Suche in Google Scholar

[3] Huang X., Zhan S., Xu H., Meng X., Xiong Y., Ultrasensitive fluorescence immunoassay for detection of ochratoxin A using catalase-mediated fluorescence quenching of CdTe QDs, Nanoscale, 2016, 8(17), 9390-9397.10.1039/C6NR01136ESuche in Google Scholar

[4] Zhou W.L., Kong W., Dou X., Zhao M., Ouyang Z., An aptamer based lateral flow strip for on-site rapid detection of ochratoxin A in Astragalus membranaceus, Journal of Chromatography B, 2016, 1022, 102-108.10.1016/j.jchromb.2016.04.016Suche in Google Scholar PubMed

[5] Taghdisi S.M., Danesh N.M., Beheshti H.R., Ramezani M., Abnous K., A novel fluorescent aptasensor based on gold and silica nanoparticles for the ultrasensitive detection of ochratoxin A, Nanoscale, 2016, 8(6), 3439-3446.10.1039/C5NR08234JSuche in Google Scholar

[6] Yang C., Lates V., Prietosimón B., Marty J.L., Yang X., Aptamer-DNAzyme hairpins for biosensing of ochratoxin A, Biosensors and Bioelectronics, 2012, 32(1), 208-212.10.1016/j.bios.2011.12.011Suche in Google Scholar PubMed

[7] Mao J., Lei S., Yang X., Xiao D., Quantification of ochratoxin A in red wines by conventional HPLC-FLD using a column packed with coreeshell particles, Food Control, 2013, 32(2), 505-511.10.1016/j.foodcont.2013.01.016Suche in Google Scholar

[8] Cao J., Zhou S., Kong W., Yang M., Wan L., Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation, Food Control, 2013, 33(2): 337-343.10.1016/j.foodcont.2013.03.023Suche in Google Scholar

[9] V. Dohnal, V. Dvořák, F. Malíř, V. Ostrý, T. Roubal, A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic, Food and Chemical Toxicology, 2013, 62, 427-431.10.1016/j.fct.2013.09.010Suche in Google Scholar PubMed

[10] Yang C., Wang Y., Marty J.L., Yang X., Aptamer-based colorimetric biosensing of ochratoxin A using unmodified gold nanoparticles indicator, Biosensors and Bioelectronics, 2011, 26(5), 2724-2727.10.1016/j.bios.2010.09.032Suche in Google Scholar PubMed

[11] Tong P., Zhang L., Xu J.J., Simply amplified electrochemical aptasensor of ochratoxin A based on exonuclease-catalyzed target recycling, Biosensors and Bioelectronics, 2011, 29(1), 97-101.10.1016/j.bios.2011.07.075Suche in Google Scholar PubMed

[12] Kim S., Lim H.B., Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A, Talanta, 2015, 140(1), 183-188.10.1016/j.talanta.2015.03.044Suche in Google Scholar PubMed

[13] Wang C., Dong X., Liu Q., Wang K., Label-free colorimetric aptasensor for sensitive detection of ochratoxin A utilizing hybridization chain reaction, Analytica Chimica Acta, 2015, 860(20), 83-88.10.1016/j.aca.2014.12.031Suche in Google Scholar PubMed

[14] Hong C.Y., Chen Y.C., Selective enrichment of ochratoxin A using human serum albumin bound magnetic beads as the concentrating probes for capillary electrophoresis/electrospray ionization-mass spectrometric analysis, Journal of Chromatography A, 2007, 1159(1-2), 250-255.10.1016/j.chroma.2007.05.026Suche in Google Scholar PubMed

[15] Novo P., Moulas G., Prazeres D.M.F., Chu V., Conde J.P., Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes, Sensors and Actuators B: Chemical, 2013, 176, 232-240.10.1016/j.snb.2012.10.038Suche in Google Scholar

[16] Yang J., Gao P., Liu Y., Li R., Ma H., Label-free photoelectron chemical immunosensor for sensitive detection of ochratoxin A, Biosensors and Bioelectronics, 2015, 64(15), 13-18.10.1016/j.bios.2014.08.025Suche in Google Scholar PubMed

[17] Zamfir L.G., Geana I., Bourigua S., Rotariu L., Bala C., Highly sensitive label-free immunosensor for ochratoxin A based on functionalized magnetic nanoparticles and EIS/SPR detection, Sensors and Actuators B: Chemical, 2011, 159(1), 178-184.10.1016/j.snb.2011.06.069Suche in Google Scholar

[18] Li M.K., Hu L.Y., Niu C.G., Huang D.W., Zeng G.M., A magnetic separation fluorescent aptasensor for highly sensitivedetection of bisphenol A, Sensors and Actuators B: Chemical, 2018, 266(1), 805-811.10.1016/j.snb.2018.03.163Suche in Google Scholar

[19] Hu L.Y., Niu C.G., Wang X.Y., Huang D.W., Zhang L., Zeng G.M., Magnetic separate turn-on fluorescent biosensor for bisphenol A based on magnetic oxidation graphene, Talanta, 2017, 168(1) 196-202.10.1016/j.talanta.2017.03.055Suche in Google Scholar PubMed

[20] Lao Y.H., Phua K.K.L., Leong K.W., Aptamer nanomedicine for cancer therapeutics: barriers and potential for translation, ACS Nano, 2015, 9(3), 2235-2254.10.1021/nn507494pSuche in Google Scholar PubMed

[21] Zhao Q., Lv Q., Wang H.L., Identification of allosteric nucleotide sites of tetramethyl rhodamine-labeled aptamer for noncompetitive aptamer-based fluorescence anisotropy detection of a small molecule, ochratoxin A, Analytical chemistry, 2014, 86(2), 1238-1245.10.1021/ac4035532Suche in Google Scholar PubMed

[22] Seung S.O., Lee B.F., Leibfarth F.A., Eisenstein M., Robb M.J., Lynd N.A., Hawker C.J., Soh H.T., Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells, Journal of the American Chemical Society, 2014, 136(42),15010-15015.10.1021/ja5079464Suche in Google Scholar PubMed PubMed Central

[23] Li M.K., Hu L.Y., Niu C.G., Huang D.W., Zeng G.M., A fluorescent DNA based probe for Hg(II) based on thymine-Hg(II)-thymine interaction and enrichment via magnetized graphene oxide, Microchimica Acta, 2018, 185(3), 207.10.1007/s00604-018-2689-6Suche in Google Scholar PubMed

[24] Gao J.W., Chen Z.Y., Mao L.B., Zhang W., Wen W., Zhang X.H., Wang S.F., Electrochemiluminescent aptasensor based on resonance energy transfer system between CdTe quantum dots and cyanine dyes for the sensitive detection of Ochratoxin A, Talanta, 2019, 199, 178-183.10.1016/j.talanta.2019.02.044Suche in Google Scholar PubMed

[25] Peng B., Tang L., Zeng G.M, Fang S.Y., Ouyang X.L., Long B. Q., Zhou Y.Y., Deng Y.C., Liu Y.N., Wang J.J., Self-powered photoelectrochemical aptasensor based on phosphorus doped porous ultrathin g-C3N4 nanosheets enhanced by surface plasmon resonance effect, Biosensors and Bioelectronics, 2018, 121, 19-26.10.1016/j.bios.2018.08.042Suche in Google Scholar PubMed

[26] Lv X.X., Li Z.Z., Niu C.G., Ruan M., Huang D.W., Zeng G.M., Determination of ammonia-oxidizing bacteria based on time-resolved fluorescence and tandem probe, Water Science and Technology, 2012, 66(6), 1361-1368.10.2166/wst.2012.335Suche in Google Scholar PubMed

[27] Wu S., Duan N., Zhu C., Ma X., Wang M., Magnetic nanobead-based immunoassay for the simultaneous detection of aflatoxin B1 and ochratoxin A using upconversion nanoparticles as multicolor labels, Biosensors and Bioelectronics, 2011, 30(1), 35-42.10.1016/j.bios.2011.08.023Suche in Google Scholar PubMed

[28] Sun Y., Xu J., Li W., Cao B., Wang D.D., Simultaneous detection of Ochratoxin A and fumonisin B1 in cereal samples using an aptamer-photonic crystal encoded suspension array, Analytical chemistry, 2014, 86(23), 11797-11802.10.1021/ac503355nSuche in Google Scholar PubMed

[29] Jiang C. M., Lan L. Y., Yao Y., Zhao F. N., Ping J. F., Recent progress in application of nanomaterial-enabled biosensors for ochratoxin A detection, Trends in Analytical Chemistry, 2018, 102, 236-249.10.1016/j.trac.2018.02.007Suche in Google Scholar

[30] Dong H. F., Wang C., Xiong Y., Lu H.T., Ju H.X., Zhang X.J., Highly sensitive andselective chemiluminescent imaging for DNA detection by ligation-mediatedrolling circle amplified synthesis of DNAzyme, Biosensors and Bioelectronics, 2013, 41(1), 348-353.10.1016/j.bios.2012.08.050Suche in Google Scholar PubMed

[31] Zhang J., Zhang X., Yang G., Chen J., Wang S., A signal-on fluorescent aptasensor based onTb3þ and structure-switching aptamer for label-free detection of ochratoxin A in wheat, Biosensors and Bioelectronics, 2013, 41(1), 704-709.10.1016/j.bios.2012.09.053Suche in Google Scholar PubMed

[32] Bonel L., Vidal J.C., Duato P., Castillo J.R., An electrochemical competitive biosensor for ochratoxin A based on a DNA biotinylated aptamer, Biosensors and Bioelectronics, 2011, 26(7), 3254-3259.10.1016/j.bios.2010.12.036Suche in Google Scholar PubMed

[33] Xu Z., Li G., Ren Y.Y., Huang H., Wen X.P., Xu Q., Fan X.T., Huang Z., Huang J.H., Xu L., A selective fluorescent probe for the detection of Cd(2+) in different buffer solutions and water, Dalton Transactions, 2016, 45(30), 12087-12093.10.1039/C6DT01398HSuche in Google Scholar PubMed

[34] Huang D.W., Niu C.G., Ruan M., Wang X.Y., Zeng G.M., Deng C.H., Highly sensitive strategy for Hg2+ detection in environmental water samples using long lifetime fluorescence quantum dots and gold nanoparticles, Environmental Science & Technology, 2013, 47(9), 4392-4398.10.1021/es302967nSuche in Google Scholar PubMed

[35] Reiter E.V., Cichna-Markl M., Chung D.H., Shim W.B., Zentek J., Determination of ochratoxin A in grains by immunoultrafiltration and HPLC-fluorescence detection after postcolumn derivatisation in an electrochemical cell, Analytical and Bioanalytical Chemistry, 2011, 400(8), 2615-2622.10.1007/s00216-011-4942-2Suche in Google Scholar PubMed

[36] Xiang J., Pi X.M., Chen X.Q., Xiang L., Yang M.H., Ren H., Shen X.J., Qi N., Deng C. Y., Integrated signal probe based aptasensor for dual-analyte detection, Biosensors and Bioelectronics, 2017, 96, 268-274.10.1016/j.bios.2017.04.039Suche in Google Scholar PubMed

[37] Guo Z., Ren J., Wang J., Wang E., Single-walled carbon nanotubes based quenching of free FAM-aptamer for selective determination of ochratoxin A, Talanta, 2011, 85(5), 2517-2521.10.1016/j.talanta.2011.08.015Suche in Google Scholar PubMed

[38] Willner I., Zayats M., Electronic aptamer-based sensors, Angewandte Chemie International Edition, 2007, 46(34), 6408-6418.10.1002/anie.200604524Suche in Google Scholar PubMed

[39] Visconti A., Bottalico A., High levels of ochratoxins A and B in moldy bread responsible for mycotoxicosis in farm animals, Journal of agricultural and food chemistry, 1983, 31(5), 1122-1123.10.1021/jf00119a050Suche in Google Scholar PubMed

Received: 2019-05-06
Accepted: 2019-08-23
Published Online: 2019-12-31

© 2019 Pinzhu Qin et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 Public License.

Artikel in diesem Heft

  1. Regular Articles
  2. Research on correlation of compositions with oestrogenic activity of Cistanche based on LC/Q-TOF-MS/MS technology
  3. Efficacy of Pyrus elaeagnifolia subsp. elaeagnifolia in acetic acid–induced colitis model
  4. Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism
  5. High efficiency liposome fusion induced by reducing undesired membrane peptides interaction
  6. Prediction of the Blood-Brain Barrier Permeability Using RP-18 Thin Layer Chromatography
  7. Phytic Acid Extracted from Rice Bran as a Growth Promoter for Euglena gracilis
  8. Development of a validated spectrofluorimetric method for assay of sotalol hydrochloride in tablets and human plasma: application for stability-indicating studies
  9. Topological Indices of Hyaluronic Acid-Paclitaxel Conjugates’ Molecular Structure in Cancer Treatment
  10. Thermodynamic properties of the bubble growth process in a pool boiling of water-ethanol mixture two-component system
  11. Critical Roles of the PI3K-Akt-mTOR Signaling Pathway in Apoptosis and Autophagy of Astrocytes Induced by Methamphetamine
  12. Characteristics of Stable Hydrogen and Oxygen Isotopes of Soil Moisture under Different Land Use in Dry Hot Valley of Yuanmou
  13. Specific, highly sensitive and simple spectrofluorimetric method for quantification of daclatasvir in HCV human plasma patients and in tablets dosage form
  14. Chromium-modified cobalt molybdenum nitrides as catalysts for ammonia synthesis
  15. Langerhans cell-like dendritic cells treated with ginsenoside Rh2 regulate the differentiation of Th1 and Th2 cells in vivo
  16. Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR
  17. Computational Analysis of new Degree-based descriptors of oxide networks
  18. The Use Of Chemical Composition And Additives To Classify Petrol And Diesel Using Gas Chromatography–Mass Spectrometry And Chemometric Analysis: A Uk Study
  19. Minimal Energy Tree with 4 Branched Vertices
  20. Jatropha seed oil derived poly(esteramide-urethane)/ fumed silica nanocomposite coatings for corrosion protection
  21. Calculating topological indices of certain OTIS interconnection networks
  22. Energy storage analysis of R125 in UIO-66 and MOF-5 nanoparticles: A molecular simulation study
  23. Velvet Antler compounds targeting major cell signaling pathways in osteosarcoma - a new insight into mediating the process of invasion and metastasis in OS
  24. Effects of Azadirachta Indica Leaf Extract, Capping Agents, on the Synthesis of Pure And Cu Doped ZnO-Nanoparticles: A Green Approach and Microbial Activity
  25. Aqueous Micro-hydration of Na+(H2O)n=1-7 Clusters: DFT Study
  26. A proposed image-based detection of methamidophos pesticide using peroxyoxalate chemiluminescence system
  27. Phytochemical screening and estrogenic activity of total glycosides of Cistanche deserticola
  28. Biological evaluation of a series of benzothiazole derivatives as mosquitocidal agents
  29. Chemical pretreatments of Trapa bispinosa's peel (TBP) biosorbent to enhance adsorption capacity for Pb(ll)
  30. Dynamic Changes in MMP1 and TIMP1 in the Antifibrotic Process of Dahuang Zhechong Pill in Rats with Liver Fibrosis
  31. The Optimization and Production of Ginkgolide B Lipid Microemulsion
  32. Photodynamic Therapy Enhanced the Antitumor Effects of Berberine on HeLa Cells
  33. Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol
  34. Correlation of Water Fluoride with Body Fluids, Dental Fluorosis and FT4, FT3 –TSH Disruption among Children in an Endemic Fluorosis area in Pakistan
  35. A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
  36. Free Radical Scavenging Activity of Essential Oil of Eugenia caryophylata from Amboina Island and Derivatives of Eugenol
  37. Effects of Blue and Red Light On Growth And Nitrate Metabolism In Pakchoi
  38. miRNA-199a-5p functions as a tumor suppressor in prolactinomas
  39. Solar photodegradation of carbamazepine from aqueous solutions using a compound parabolic concentrator equipped with a sun tracking system
  40. Influence of sub-inhibitory concentration of selected plant essential oils on the physical and biochemical properties of Pseudomonas orientalis
  41. Preparation and spectroscopic studies of Fe(II), Ru(II), Pd(II) and Zn(II) complexes of Schiff base containing terephthalaldehyde and their transfer hydrogenation and Suzuki-Miyaura coupling reaction
  42. Complex formation in a liquid-liquid extraction-chromogenic system for vanadium(IV)
  43. Synthesis, characterization (IR, 1H, 13C & 31P NMR), fungicidal, herbicidal and molecular docking evaluation of steroid phosphorus compounds
  44. Analysis and Biological Evaluation of Arisaema Amuremse Maxim Essential Oil
  45. A preliminary assessment of potential ecological risk and soil contamination by heavy metals around a cement factory, western Saudi Arabia
  46. Anti- inflammatory effect of Prunus tomentosa Thunb total flavones in LPS-induced RAW264.7 cells
  47. Collaborative Influence of Elevated CO2 Concentration and High Temperature on Potato Biomass Accumulation and Characteristics
  48. Methods of extraction, physicochemical properties of alginates and their applications in biomedical field – a review
  49. Characteristics of liposomes derived from egg yolk
  50. Preparation of ternary ZnO/Ag/cellulose and its enhanced photocatalytic degradation property on phenol and benzene in VOCs
  51. Influence of Human Serum Albumin Glycation on the Binding Affinities for Natural Flavonoids
  52. Synthesis and antioxidant activity of 2-methylthio-pyrido[3,2-e][1,2,4] triazolo[1,5-a]pyrimidines
  53. Comparative study on the antioxidant activities of ten common flower teas from China
  54. Molecular Properties of Symmetrical Networks Using Topological Polynomials
  55. Synthesis of Co3O4 Nano Aggregates by Co-precipitation Method and its Catalytic and Fuel Additive Applications
  56. Phytochemical analysis, Antioxidant and Antiprotoscolices potential of ethanol extracts of selected plants species against Echinococcus granulosus: In-vitro study
  57. Silver nanoparticles enhanced fluorescence for sensitive determination of fluoroquinolones in water solutions
  58. Simultaneous Quantification of the New Psychoactive Substances 3-FMC, 3-FPM, 4-CEC, and 4-BMC in Human Blood using GC-MS
  59. Biodiesel Production by Lipids From Indonesian strain of Microalgae Chlorella vulgaris
  60. Miscibility studies of polystyrene/polyvinyl chloride blend in presence of organoclay
  61. Antibacterial Activities of Transition Metal complexes of Mesocyclic Amidine 1,4-diazacycloheptane (DACH)
  62. Novel 1,8-Naphthyridine Derivatives: Design, Synthesis and in vitro screening of their cytotoxic activity against MCF7 cell line
  63. Investigation of Stress Corrosion Cracking Behaviour of Mg-Al-Zn Alloys in Different pH Environments by SSRT Method
  64. Various Combinations of Flame Retardants for Poly (vinyl chloride)
  65. Phenolic compounds and biological activities of rye (Secale cereale L.) grains
  66. Oxidative degradation of gentamicin present in water by an electro-Fenton process and biodegradability improvement
  67. Optimizing Suitable Conditions for the Removal of Ammonium Nitrogen by a Microbe Isolated from Chicken Manure
  68. Anti-inflammatory, antipyretic, analgesic, and antioxidant activities of Haloxylon salicornicum aqueous fraction
  69. The anti-corrosion behaviour of Satureja montana L. extract on iron in NaCl solution
  70. Interleukin-4, hemopexin, and lipoprotein-associated phospholipase A2 are significantly increased in patients with unstable carotid plaque
  71. A comparative study of the crystal structures of 2-(4-(2-(4-(3-chlorophenyl)pipera -zinyl)ethyl) benzyl)isoindoline-1,3-dione by synchrotron radiation X-ray powder diffraction and single-crystal X-ray diffraction
  72. Conceptual DFT as a Novel Chemoinformatics Tool for Studying the Chemical Reactivity Properties of the Amatoxin Family of Fungal Peptides
  73. Occurrence of Aflatoxin M1 in Milk-based Mithae samples from Pakistan
  74. Kinetics of Iron Removal From Ti-Extraction Blast Furnace Slag by Chlorination Calcination
  75. Increasing the activity of DNAzyme based on the telomeric sequence: 2’-OMe-RNA and LNA modifications
  76. Exploring the optoelectronic properties of a chromene-appended pyrimidone derivative for photovoltaic applications
  77. Effect of He Qi San on DNA Methylation in Type 2 Diabetes Mellitus Patients with Phlegm-blood Stasis Syndrome
  78. Cyclodextrin potentiometric sensors based on selective recognition sites for procainamide: Comparative and theoretical study
  79. Greener synthesis of dimethyl carbonate from carbon dioxide and methanol using a tunable ionic liquid catalyst
  80. Nonisothermal Cold Crystallization Kinetics of Poly(lactic acid)/Bacterial Poly(hydroxyoctanoate) (PHO)/Talc
  81. Enhanced adsorption of sulfonamide antibiotics in water by modified biochar derived from bagasse
  82. Study on the Mechanism of Shugan Xiaozhi Fang on Cells with Non-alcoholic Fatty Liver Disease
  83. Comparative Effects of Salt and Alkali Stress on Antioxidant System in Cotton (Gossypium Hirsutum L.) Leaves
  84. Optimization of chromatographic systems for analysis of selected psychotropic drugs and their metabolites in serum and saliva by HPLC in order to monitor therapeutic drugs
  85. Electrocatalytic Properties of Ni-Doped BaFe12O19 for Oxygen Evolution in Alkaline Solution
  86. Study on the removal of high contents of ammonium from piggery wastewater by clinoptilolite and the corresponding mechanisms
  87. Phytochemistry and toxicological assessment of Bryonia dioica roots used in north-African alternative medicine
  88. The essential oil composition of selected Hemerocallis cultivars and their biological activity
  89. Mechanical Properties of Carbon Fiber Reinforced Nanocrystalline Nickel Composite Electroforming Deposit
  90. Anti-c-myc efficacy block EGFL7 induced prolactinoma tumorigenesis
  91. Topical Issue on Applications of Mathematics in Chemistry
  92. Zagreb Connection Number Index of Nanotubes and Regular Hexagonal Lattice
  93. The Sanskruti index of trees and unicyclic graphs
  94. Valency-based molecular descriptors of Bakelite network BNmn
  95. Computing Topological Indices for Para-Line Graphs of Anthracene
  96. Zagreb Polynomials and redefined Zagreb indices of Dendrimers and Polyomino Chains
  97. Topological Descriptor of 2-Dimensional Silicon Carbons and Their Applications
  98. Topological invariants for the line graphs of some classes of graphs
  99. Words for maximal Subgroups of Fi24‘
  100. Generators of Maximal Subgroups of Harada-Norton and some Linear Groups
  101. Special Issue on POKOCHA 2018
  102. Influence of Production Parameters on the Content of Polyphenolic Compounds in Extruded Porridge Enriched with Chokeberry Fruit (Aronia melanocarpa (Michx.) Elliott)
  103. Effects of Supercritical Carbon Dioxide Extraction (SC-CO2) on the content of tiliroside in the extracts from Tilia L. flowers
  104. Impact of xanthan gum addition on phenolic acids composition and selected properties of new gluten-free maize-field bean pasta
  105. Impact of storage temperature and time on Moldavian dragonhead oil – spectroscopic and chemometric analysis
  106. The effect of selected substances on the stability of standard solutions in voltammetric analysis of ascorbic acid in fruit juices
  107. Determination of the content of Pb, Cd, Cu, Zn in dairy products from various regions of Poland
  108. Special Issue on IC3PE 2018 Conference
  109. The Photocatalytic Activity of Zns-TiO2 on a Carbon Fiber Prepared by Chemical Bath Deposition
  110. N-octyl chitosan derivatives as amphiphilic carrier agents for herbicide formulations
  111. Kinetics and Mechanistic Study of Hydrolysis of Adenosine Monophosphate Disodium Salt (AMPNa2) in Acidic and Alkaline Media
  112. Antimalarial Activity of Andrographis Paniculata Ness‘s N-hexane Extract and Its Major Compounds
  113. Special Issue on ABB2018 Conference
  114. Special Issue on ICCESEN 2017
  115. Theoretical Diagnostics of Second and Third-order Hyperpolarizabilities of Several Acid Derivatives
  116. Determination of Gamma Rays Efficiency Against Rhizoctonia solani in Potatoes
  117. Studies On Compatibilization Of Recycled Polyethylene/Thermoplastic Starch Blends By Using Different Compatibilizer
  118. Liquid−Liquid Extraction of Linalool from Methyl Eugenol with 1-Ethyl-3-methylimidazolium Hydrogen Sulfate [EMIM][HSO4] Ionic Liquid
  119. Synthesis of Graphene Oxide Through Ultrasonic Assisted Electrochemical Exfoliation
  120. Special Issue on ISCMP 2018
  121. Synthesis and antiproliferative evaluation of some 1,4-naphthoquinone derivatives against human cervical cancer cells
  122. The influence of the grafted aryl groups on the solvation properties of the graphyne and graphdiyne - a MD study
  123. Electrochemical modification of platinum and glassy carbon surfaces with pyridine layers and their use as complexing agents for copper (II) ions
  124. Effect of Electrospinning Process on Total Antioxidant Activity of Electrospun Nanofibers Containing Grape Seed Extract
  125. Effect Of Thermal Treatment Of Trepel At Temperature Range 800-1200˚C
  126. Topical Issue on Agriculture
  127. The effect of Cladophora glomerata exudates on the amino acid composition of Cladophora fracta and Rhizoclonium sp.
  128. Influence of the Static Magnetic Field and Algal Extract on the Germination of Soybean Seeds
  129. The use of UV-induced fluorescence for the assessment of homogeneity of granular mixtures
  130. The use of microorganisms as bio-fertilizers in the cultivation of white lupine
  131. Lyophilized apples on flax oil and ethyl esters of flax oil - stability and antioxidant evaluation
  132. Production of phosphorus biofertilizer based on the renewable materials in large laboratory scale
  133. Human health risk assessment of potential toxic elements in paddy soil and rice (Oryza sativa) from Ugbawka fields, Enugu, Nigeria
  134. Recovery of phosphates(V) from wastewaters of different chemical composition
  135. Special Issue on the 4th Green Chemistry 2018
  136. Dead zone for hydrogenation of propylene reaction carried out on commercial catalyst pellets
  137. Improved thermally stable oligoetherols from 6-aminouracil, ethylene carbonate and boric acid
  138. The role of a chemical loop in removal of hazardous contaminants from coke oven wastewater during its treatment
  139. Combating paraben pollution in surface waters with a variety of photocatalyzed systems: Looking for the most efficient technology
  140. Special Issue on Chemistry Today for Tomorrow 2019
  141. Applying Discriminant and Cluster Analyses to Separate Allergenic from Non-allergenic Proteins
  142. Chemometric Expertise Of Clinical Monitoring Data Of Prolactinoma Patients
  143. Chemomertic Risk Assessment of Soil Pollution
  144. New composite sorbent for speciation analysis of soluble chromium in textiles
  145. Photocatalytic activity of NiFe2O4 and Zn0.5Ni0.5Fe2O4 modified by Eu(III) and Tb(III) for decomposition of Malachite Green
  146. Photophysical and antibacterial activity of light-activated quaternary eosin Y
  147. Spectral properties and biological activity of La(III) and Nd(III) Monensinates
  148. Special Issue on Monitoring, Risk Assessment and Sustainable Management for the Exposure to Environmental Toxins
  149. Soil organic carbon mineralization in relation to microbial dynamics in subtropical red soils dominated by differently sized aggregates
  150. A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis
  151. Special Issue on 13th JCC 2018
  152. Fluorescence study of 5-nitroisatin Schiff base immobilized on SBA-15 for sensing Fe3+
  153. Thermal and Morphology Properties of Cellulose Nanofiber from TEMPO-oxidized Lower part of Empty Fruit Bunches (LEFB)
  154. Encapsulation of Vitamin C in Sesame Liposomes: Computational and Experimental Studies
  155. A comparative study of the utilization of synthetic foaming agent and aluminum powder as pore-forming agents in lightweight geopolymer synthesis
  156. Synthesis of high surface area mesoporous silica SBA-15 by adjusting hydrothermal treatment time and the amount of polyvinyl alcohol
  157. Review of large-pore mesostructured cellular foam (MCF) silica and its applications
  158. Ion Exchange of Benzoate in Ni-Al-Benzoate Layered Double Hydroxide by Amoxicillin
  159. Synthesis And Characterization Of CoMo/Mordenite Catalyst For Hydrotreatment Of Lignin Compound Models
  160. Production of Biodiesel from Nyamplung (Calophyllum inophyllum L.) using Microwave with CaO Catalyst from Eggshell Waste: Optimization of Transesterification Process Parameters
  161. The Study of the Optical Properties of C60 Fullerene in Different Organic Solvents
  162. Composite Material Consisting of HKUST-1 and Indonesian Activated Natural Zeolite and its Application in CO2 Capture
  163. Topical Issue on Environmental Chemistry
  164. Ionic liquids modified cobalt/ZSM-5 as a highly efficient catalyst for enhancing the selectivity towards KA oil in the aerobic oxidation of cyclohexane
  165. Application of Thermal Resistant Gemini Surfactants in Highly Thixotropic Water-in-oil Drilling Fluid System
  166. Screening Study on Rheological Behavior and Phase Transition Point of Polymer-containing Fluids produced under the Oil Freezing Point Temperature
  167. The Chemical Softening Effect and Mechanism of Low Rank Coal Soaked in Alkaline Solution
  168. The Influence Of NO/O2 On The NOx Storage Properties Over A Pt-Ba-Ce/γ-Al2O3 Catalyst
  169. Special Issue on the International conference CosCI 2018
  170. Design of SiO2/TiO2 that Synergistically Increases The Hydrophobicity of Methyltrimethoxysilane Coated Glass
  171. Antidiabetes and Antioxidant agents from Clausena excavata root as medicinal plant of Myanmar
  172. Development of a Gold Immunochromatographic Assay Method Using Candida Biofilm Antigen as a Bioreceptor for Candidiasis in Rats
  173. Special Issue on Applied Biochemistry and Biotechnology 2019
  174. Adsorption of copper ions on Magnolia officinalis residues after solid-phase fermentation with Phanerochaete chrysosporium
  175. Erratum
  176. Erratum to: Sand Dune Characterization For Preparing Metallurgical Grade Silicon
https://doi.org/10.1515/chem-2019-0140
Schlagwörter für diesen Artikel
ochratoxin A; aptamer; fluorescence; magnetic particles; reused
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Regular Articles
  2. Research on correlation of compositions with oestrogenic activity of Cistanche based on LC/Q-TOF-MS/MS technology
  3. Efficacy of Pyrus elaeagnifolia subsp. elaeagnifolia in acetic acid–induced colitis model
  4. Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism
  5. High efficiency liposome fusion induced by reducing undesired membrane peptides interaction
  6. Prediction of the Blood-Brain Barrier Permeability Using RP-18 Thin Layer Chromatography
  7. Phytic Acid Extracted from Rice Bran as a Growth Promoter for Euglena gracilis
  8. Development of a validated spectrofluorimetric method for assay of sotalol hydrochloride in tablets and human plasma: application for stability-indicating studies
  9. Topological Indices of Hyaluronic Acid-Paclitaxel Conjugates’ Molecular Structure in Cancer Treatment
  10. Thermodynamic properties of the bubble growth process in a pool boiling of water-ethanol mixture two-component system
  11. Critical Roles of the PI3K-Akt-mTOR Signaling Pathway in Apoptosis and Autophagy of Astrocytes Induced by Methamphetamine
  12. Characteristics of Stable Hydrogen and Oxygen Isotopes of Soil Moisture under Different Land Use in Dry Hot Valley of Yuanmou
  13. Specific, highly sensitive and simple spectrofluorimetric method for quantification of daclatasvir in HCV human plasma patients and in tablets dosage form
  14. Chromium-modified cobalt molybdenum nitrides as catalysts for ammonia synthesis
  15. Langerhans cell-like dendritic cells treated with ginsenoside Rh2 regulate the differentiation of Th1 and Th2 cells in vivo
  16. Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR
  17. Computational Analysis of new Degree-based descriptors of oxide networks
  18. The Use Of Chemical Composition And Additives To Classify Petrol And Diesel Using Gas Chromatography–Mass Spectrometry And Chemometric Analysis: A Uk Study
  19. Minimal Energy Tree with 4 Branched Vertices
  20. Jatropha seed oil derived poly(esteramide-urethane)/ fumed silica nanocomposite coatings for corrosion protection
  21. Calculating topological indices of certain OTIS interconnection networks
  22. Energy storage analysis of R125 in UIO-66 and MOF-5 nanoparticles: A molecular simulation study
  23. Velvet Antler compounds targeting major cell signaling pathways in osteosarcoma - a new insight into mediating the process of invasion and metastasis in OS
  24. Effects of Azadirachta Indica Leaf Extract, Capping Agents, on the Synthesis of Pure And Cu Doped ZnO-Nanoparticles: A Green Approach and Microbial Activity
  25. Aqueous Micro-hydration of Na+(H2O)n=1-7 Clusters: DFT Study
  26. A proposed image-based detection of methamidophos pesticide using peroxyoxalate chemiluminescence system
  27. Phytochemical screening and estrogenic activity of total glycosides of Cistanche deserticola
  28. Biological evaluation of a series of benzothiazole derivatives as mosquitocidal agents
  29. Chemical pretreatments of Trapa bispinosa's peel (TBP) biosorbent to enhance adsorption capacity for Pb(ll)
  30. Dynamic Changes in MMP1 and TIMP1 in the Antifibrotic Process of Dahuang Zhechong Pill in Rats with Liver Fibrosis
  31. The Optimization and Production of Ginkgolide B Lipid Microemulsion
  32. Photodynamic Therapy Enhanced the Antitumor Effects of Berberine on HeLa Cells
  33. Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol
  34. Correlation of Water Fluoride with Body Fluids, Dental Fluorosis and FT4, FT3 –TSH Disruption among Children in an Endemic Fluorosis area in Pakistan
  35. A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
  36. Free Radical Scavenging Activity of Essential Oil of Eugenia caryophylata from Amboina Island and Derivatives of Eugenol
  37. Effects of Blue and Red Light On Growth And Nitrate Metabolism In Pakchoi
  38. miRNA-199a-5p functions as a tumor suppressor in prolactinomas
  39. Solar photodegradation of carbamazepine from aqueous solutions using a compound parabolic concentrator equipped with a sun tracking system
  40. Influence of sub-inhibitory concentration of selected plant essential oils on the physical and biochemical properties of Pseudomonas orientalis
  41. Preparation and spectroscopic studies of Fe(II), Ru(II), Pd(II) and Zn(II) complexes of Schiff base containing terephthalaldehyde and their transfer hydrogenation and Suzuki-Miyaura coupling reaction
  42. Complex formation in a liquid-liquid extraction-chromogenic system for vanadium(IV)
  43. Synthesis, characterization (IR, 1H, 13C & 31P NMR), fungicidal, herbicidal and molecular docking evaluation of steroid phosphorus compounds
  44. Analysis and Biological Evaluation of Arisaema Amuremse Maxim Essential Oil
  45. A preliminary assessment of potential ecological risk and soil contamination by heavy metals around a cement factory, western Saudi Arabia
  46. Anti- inflammatory effect of Prunus tomentosa Thunb total flavones in LPS-induced RAW264.7 cells
  47. Collaborative Influence of Elevated CO2 Concentration and High Temperature on Potato Biomass Accumulation and Characteristics
  48. Methods of extraction, physicochemical properties of alginates and their applications in biomedical field – a review
  49. Characteristics of liposomes derived from egg yolk
  50. Preparation of ternary ZnO/Ag/cellulose and its enhanced photocatalytic degradation property on phenol and benzene in VOCs
  51. Influence of Human Serum Albumin Glycation on the Binding Affinities for Natural Flavonoids
  52. Synthesis and antioxidant activity of 2-methylthio-pyrido[3,2-e][1,2,4] triazolo[1,5-a]pyrimidines
  53. Comparative study on the antioxidant activities of ten common flower teas from China
  54. Molecular Properties of Symmetrical Networks Using Topological Polynomials
  55. Synthesis of Co3O4 Nano Aggregates by Co-precipitation Method and its Catalytic and Fuel Additive Applications
  56. Phytochemical analysis, Antioxidant and Antiprotoscolices potential of ethanol extracts of selected plants species against Echinococcus granulosus: In-vitro study
  57. Silver nanoparticles enhanced fluorescence for sensitive determination of fluoroquinolones in water solutions
  58. Simultaneous Quantification of the New Psychoactive Substances 3-FMC, 3-FPM, 4-CEC, and 4-BMC in Human Blood using GC-MS
  59. Biodiesel Production by Lipids From Indonesian strain of Microalgae Chlorella vulgaris
  60. Miscibility studies of polystyrene/polyvinyl chloride blend in presence of organoclay
  61. Antibacterial Activities of Transition Metal complexes of Mesocyclic Amidine 1,4-diazacycloheptane (DACH)
  62. Novel 1,8-Naphthyridine Derivatives: Design, Synthesis and in vitro screening of their cytotoxic activity against MCF7 cell line
  63. Investigation of Stress Corrosion Cracking Behaviour of Mg-Al-Zn Alloys in Different pH Environments by SSRT Method
  64. Various Combinations of Flame Retardants for Poly (vinyl chloride)
  65. Phenolic compounds and biological activities of rye (Secale cereale L.) grains
  66. Oxidative degradation of gentamicin present in water by an electro-Fenton process and biodegradability improvement
  67. Optimizing Suitable Conditions for the Removal of Ammonium Nitrogen by a Microbe Isolated from Chicken Manure
  68. Anti-inflammatory, antipyretic, analgesic, and antioxidant activities of Haloxylon salicornicum aqueous fraction
  69. The anti-corrosion behaviour of Satureja montana L. extract on iron in NaCl solution
  70. Interleukin-4, hemopexin, and lipoprotein-associated phospholipase A2 are significantly increased in patients with unstable carotid plaque
  71. A comparative study of the crystal structures of 2-(4-(2-(4-(3-chlorophenyl)pipera -zinyl)ethyl) benzyl)isoindoline-1,3-dione by synchrotron radiation X-ray powder diffraction and single-crystal X-ray diffraction
  72. Conceptual DFT as a Novel Chemoinformatics Tool for Studying the Chemical Reactivity Properties of the Amatoxin Family of Fungal Peptides
  73. Occurrence of Aflatoxin M1 in Milk-based Mithae samples from Pakistan
  74. Kinetics of Iron Removal From Ti-Extraction Blast Furnace Slag by Chlorination Calcination
  75. Increasing the activity of DNAzyme based on the telomeric sequence: 2’-OMe-RNA and LNA modifications
  76. Exploring the optoelectronic properties of a chromene-appended pyrimidone derivative for photovoltaic applications
  77. Effect of He Qi San on DNA Methylation in Type 2 Diabetes Mellitus Patients with Phlegm-blood Stasis Syndrome
  78. Cyclodextrin potentiometric sensors based on selective recognition sites for procainamide: Comparative and theoretical study
  79. Greener synthesis of dimethyl carbonate from carbon dioxide and methanol using a tunable ionic liquid catalyst
  80. Nonisothermal Cold Crystallization Kinetics of Poly(lactic acid)/Bacterial Poly(hydroxyoctanoate) (PHO)/Talc
  81. Enhanced adsorption of sulfonamide antibiotics in water by modified biochar derived from bagasse
  82. Study on the Mechanism of Shugan Xiaozhi Fang on Cells with Non-alcoholic Fatty Liver Disease
  83. Comparative Effects of Salt and Alkali Stress on Antioxidant System in Cotton (Gossypium Hirsutum L.) Leaves
  84. Optimization of chromatographic systems for analysis of selected psychotropic drugs and their metabolites in serum and saliva by HPLC in order to monitor therapeutic drugs
  85. Electrocatalytic Properties of Ni-Doped BaFe12O19 for Oxygen Evolution in Alkaline Solution
  86. Study on the removal of high contents of ammonium from piggery wastewater by clinoptilolite and the corresponding mechanisms
  87. Phytochemistry and toxicological assessment of Bryonia dioica roots used in north-African alternative medicine
  88. The essential oil composition of selected Hemerocallis cultivars and their biological activity
  89. Mechanical Properties of Carbon Fiber Reinforced Nanocrystalline Nickel Composite Electroforming Deposit
  90. Anti-c-myc efficacy block EGFL7 induced prolactinoma tumorigenesis
  91. Topical Issue on Applications of Mathematics in Chemistry
  92. Zagreb Connection Number Index of Nanotubes and Regular Hexagonal Lattice
  93. The Sanskruti index of trees and unicyclic graphs
  94. Valency-based molecular descriptors of Bakelite network BNmn
  95. Computing Topological Indices for Para-Line Graphs of Anthracene
  96. Zagreb Polynomials and redefined Zagreb indices of Dendrimers and Polyomino Chains
  97. Topological Descriptor of 2-Dimensional Silicon Carbons and Their Applications
  98. Topological invariants for the line graphs of some classes of graphs
  99. Words for maximal Subgroups of Fi24‘
  100. Generators of Maximal Subgroups of Harada-Norton and some Linear Groups
  101. Special Issue on POKOCHA 2018
  102. Influence of Production Parameters on the Content of Polyphenolic Compounds in Extruded Porridge Enriched with Chokeberry Fruit (Aronia melanocarpa (Michx.) Elliott)
  103. Effects of Supercritical Carbon Dioxide Extraction (SC-CO2) on the content of tiliroside in the extracts from Tilia L. flowers
  104. Impact of xanthan gum addition on phenolic acids composition and selected properties of new gluten-free maize-field bean pasta
  105. Impact of storage temperature and time on Moldavian dragonhead oil – spectroscopic and chemometric analysis
  106. The effect of selected substances on the stability of standard solutions in voltammetric analysis of ascorbic acid in fruit juices
  107. Determination of the content of Pb, Cd, Cu, Zn in dairy products from various regions of Poland
  108. Special Issue on IC3PE 2018 Conference
  109. The Photocatalytic Activity of Zns-TiO2 on a Carbon Fiber Prepared by Chemical Bath Deposition
  110. N-octyl chitosan derivatives as amphiphilic carrier agents for herbicide formulations
  111. Kinetics and Mechanistic Study of Hydrolysis of Adenosine Monophosphate Disodium Salt (AMPNa2) in Acidic and Alkaline Media
  112. Antimalarial Activity of Andrographis Paniculata Ness‘s N-hexane Extract and Its Major Compounds
  113. Special Issue on ABB2018 Conference
  114. Special Issue on ICCESEN 2017
  115. Theoretical Diagnostics of Second and Third-order Hyperpolarizabilities of Several Acid Derivatives
  116. Determination of Gamma Rays Efficiency Against Rhizoctonia solani in Potatoes
  117. Studies On Compatibilization Of Recycled Polyethylene/Thermoplastic Starch Blends By Using Different Compatibilizer
  118. Liquid−Liquid Extraction of Linalool from Methyl Eugenol with 1-Ethyl-3-methylimidazolium Hydrogen Sulfate [EMIM][HSO4] Ionic Liquid
  119. Synthesis of Graphene Oxide Through Ultrasonic Assisted Electrochemical Exfoliation
  120. Special Issue on ISCMP 2018
  121. Synthesis and antiproliferative evaluation of some 1,4-naphthoquinone derivatives against human cervical cancer cells
  122. The influence of the grafted aryl groups on the solvation properties of the graphyne and graphdiyne - a MD study
  123. Electrochemical modification of platinum and glassy carbon surfaces with pyridine layers and their use as complexing agents for copper (II) ions
  124. Effect of Electrospinning Process on Total Antioxidant Activity of Electrospun Nanofibers Containing Grape Seed Extract
  125. Effect Of Thermal Treatment Of Trepel At Temperature Range 800-1200˚C
  126. Topical Issue on Agriculture
  127. The effect of Cladophora glomerata exudates on the amino acid composition of Cladophora fracta and Rhizoclonium sp.
  128. Influence of the Static Magnetic Field and Algal Extract on the Germination of Soybean Seeds
  129. The use of UV-induced fluorescence for the assessment of homogeneity of granular mixtures
  130. The use of microorganisms as bio-fertilizers in the cultivation of white lupine
  131. Lyophilized apples on flax oil and ethyl esters of flax oil - stability and antioxidant evaluation
  132. Production of phosphorus biofertilizer based on the renewable materials in large laboratory scale
  133. Human health risk assessment of potential toxic elements in paddy soil and rice (Oryza sativa) from Ugbawka fields, Enugu, Nigeria
  134. Recovery of phosphates(V) from wastewaters of different chemical composition
  135. Special Issue on the 4th Green Chemistry 2018
  136. Dead zone for hydrogenation of propylene reaction carried out on commercial catalyst pellets
  137. Improved thermally stable oligoetherols from 6-aminouracil, ethylene carbonate and boric acid
  138. The role of a chemical loop in removal of hazardous contaminants from coke oven wastewater during its treatment
  139. Combating paraben pollution in surface waters with a variety of photocatalyzed systems: Looking for the most efficient technology
  140. Special Issue on Chemistry Today for Tomorrow 2019
  141. Applying Discriminant and Cluster Analyses to Separate Allergenic from Non-allergenic Proteins
  142. Chemometric Expertise Of Clinical Monitoring Data Of Prolactinoma Patients
  143. Chemomertic Risk Assessment of Soil Pollution
  144. New composite sorbent for speciation analysis of soluble chromium in textiles
  145. Photocatalytic activity of NiFe2O4 and Zn0.5Ni0.5Fe2O4 modified by Eu(III) and Tb(III) for decomposition of Malachite Green
  146. Photophysical and antibacterial activity of light-activated quaternary eosin Y
  147. Spectral properties and biological activity of La(III) and Nd(III) Monensinates
  148. Special Issue on Monitoring, Risk Assessment and Sustainable Management for the Exposure to Environmental Toxins
  149. Soil organic carbon mineralization in relation to microbial dynamics in subtropical red soils dominated by differently sized aggregates
  150. A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis
  151. Special Issue on 13th JCC 2018
  152. Fluorescence study of 5-nitroisatin Schiff base immobilized on SBA-15 for sensing Fe3+
  153. Thermal and Morphology Properties of Cellulose Nanofiber from TEMPO-oxidized Lower part of Empty Fruit Bunches (LEFB)
  154. Encapsulation of Vitamin C in Sesame Liposomes: Computational and Experimental Studies
  155. A comparative study of the utilization of synthetic foaming agent and aluminum powder as pore-forming agents in lightweight geopolymer synthesis
  156. Synthesis of high surface area mesoporous silica SBA-15 by adjusting hydrothermal treatment time and the amount of polyvinyl alcohol
  157. Review of large-pore mesostructured cellular foam (MCF) silica and its applications
  158. Ion Exchange of Benzoate in Ni-Al-Benzoate Layered Double Hydroxide by Amoxicillin
  159. Synthesis And Characterization Of CoMo/Mordenite Catalyst For Hydrotreatment Of Lignin Compound Models
  160. Production of Biodiesel from Nyamplung (Calophyllum inophyllum L.) using Microwave with CaO Catalyst from Eggshell Waste: Optimization of Transesterification Process Parameters
  161. The Study of the Optical Properties of C60 Fullerene in Different Organic Solvents
  162. Composite Material Consisting of HKUST-1 and Indonesian Activated Natural Zeolite and its Application in CO2 Capture
  163. Topical Issue on Environmental Chemistry
  164. Ionic liquids modified cobalt/ZSM-5 as a highly efficient catalyst for enhancing the selectivity towards KA oil in the aerobic oxidation of cyclohexane
  165. Application of Thermal Resistant Gemini Surfactants in Highly Thixotropic Water-in-oil Drilling Fluid System
  166. Screening Study on Rheological Behavior and Phase Transition Point of Polymer-containing Fluids produced under the Oil Freezing Point Temperature
  167. The Chemical Softening Effect and Mechanism of Low Rank Coal Soaked in Alkaline Solution
  168. The Influence Of NO/O2 On The NOx Storage Properties Over A Pt-Ba-Ce/γ-Al2O3 Catalyst
  169. Special Issue on the International conference CosCI 2018
  170. Design of SiO2/TiO2 that Synergistically Increases The Hydrophobicity of Methyltrimethoxysilane Coated Glass
  171. Antidiabetes and Antioxidant agents from Clausena excavata root as medicinal plant of Myanmar
  172. Development of a Gold Immunochromatographic Assay Method Using Candida Biofilm Antigen as a Bioreceptor for Candidiasis in Rats
  173. Special Issue on Applied Biochemistry and Biotechnology 2019
  174. Adsorption of copper ions on Magnolia officinalis residues after solid-phase fermentation with Phanerochaete chrysosporium
  175. Erratum
  176. Erratum to: Sand Dune Characterization For Preparing Metallurgical Grade Silicon
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2025 De Gruyter Brill
Heruntergeladen am 17.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/chem-2019-0140/html
Button zum nach oben scrollen