Home Medicine Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human mesenchymal stem cells
Article Open Access

Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human mesenchymal stem cells

  • Yijie Chen , Wenqi Sha , Yifan Zhang , Wanyi Kou , Liu Yang , Ruixin Guo , Chenyang Li , Junjie Zhao and Zhenghui Wang EMAIL logo
Published/Copyright: November 15, 2024
Download Article (PDF)
Open Medicine
From the journal Open Medicine Volume 19 Issue 1

Abstract

Objective

Dysregulation of chondrogenic differentiation is associated with osteoarthritis (OA). The myokine irisin is beneficial in OA treatment; yet, the underlying mechanism is not fully understood. Long noncoding RNAs (lncRNAs) act as important regulators of chondrocyte differentiation. This study was conducted to address the role of lncRNAs in mediating irisin-induced chondrocyte differentiation.

Methods

We investigated the irisin-regulated lncRNA profile change in human mesenchymal stem cells (MSCs) using published whole transcriptome sequencing data. We predicted their potential targets and competitive endogenous RNA (ceRNA) prediction and analyzed their molecular functions using functional enrichment analysis.

Results

More differentially expressed lncRNAs (DElncRNAs) were observed in irisin-treated samples. The top irisin-induced lncRNAs were associated with OA or chondrogenic differentiation, including XIST, PAX8-AS1, CASC15, LINC01618, and DLX6-AS1. The DEGs co-expressed with DElncRNAs were enriched in skeletal system development, extracellular matrix (ECM) organization, cell adhesion, and inflammation associated pathways. Several lncRNAs likely acted as ceRNAs to regulate downstream mRNAs including ROR2 and SORBS1 in in OA or chondrogenic differentiation.

Conclusions

We demonstrate the global regulation of lncRNAs by irisin during chondrogenic differentiation of human MSCs. Further study is required to characterize the key irisin-regulated lncRNAs in chondrogenic differentiation

Keywords: irisin; chondrogenic differentiation; lncRNAs; co-expression; ceRNAs

1 Introduction

Cartilage injury is a common clinical problem. Due to the weak regeneration potential of chondrocytes, in vitro cartilage tissue engineering holds promise in the repair of damaged cartilage [1]. However, the dedifferentiation of chondrocytes is an obstacle to the generation of tissue-engineered cartilage [2]. During in vitro culture, chondrocytes can lose their original phenotypes, exhibiting remarkable molecular and morphological changes [3,4]. The dedifferentiation of chondrocytes is also involved in the pathogenesis of osteoarthritis (OA) [5]. The transcription factor SOX9 plays a crucial role in the suppression of chondrocyte dedifferentiation [6,7]. Lentiviral delivery of exogenous SOX9 has been shown to inhibit dedifferentiation and hypertrophy of chondrocytes [8]. A better understanding of the molecular mechanism governing chondrocyte differentiation is of importance in cartilage tissue engineering.

Increasing evidence indicates long noncoding RNAs (lncRNAs) as important regulators of chondrocyte differentiation [9]. lncRNAs are a class of regulatory RNAs implicated in various biological processes including chondrocyte differentiation [10,11,12]. lncRNA ROCR deficiency disrupts human mesenchymal stem cell (MSC) chondrogenesis by reducing SOX9 expression in a cis-manner [13]. lncRNA-MEG3 interacts with the methyltransferase EZH2 to epigenetically inhibit TRIB2 expression, consequently blocking the chondrogenic differentiation of synovium-derived MSCs (SMSCs) [14]. lncRNA-DANCR is upregulated by SOX4 to promote the proliferation and chondrogenesis of SMSCs through interaction with and stabilization of myc protein [15]. lncRNAs can also act as competitive endogenous RNAs (ceRNAs) to modulate chondrogenic differentiation, such as ADAMTS9-AS2 [16] and DNM3OS [17].

The myokine irisin shows the ability to enhance chondrocyte differentiation [18,19,20]. It has been documented that irisin stimulates the proliferation and anabolism of human osteoarthritic chondrocytes through inactivation of p38, Akt, JNK, and NF-κB signaling [18]. Irisin affects all stages of cartilage development and ameliorates OA progression by decreasing cartilage degradation and inflammation [19]. Irisin potentiates osteogenic differentiation of bone marrow MSCs through the Wnt/β-catenin signaling pathway [20]. The exposure to irisin leads to proteomic and transcriptomic changes in different cellular contexts [21,22,23]. However, the lncRNA profile change related to irisin-induced chondrogenic differentiation has not been explored.

In this study, we hypothesized that irisin could promote chondrogenic differentiation through the modulation of key lncRNA mediators. To address this, we analyzed the lncRNA profile in irisin-treated human MSCs based on RNA sequencing data reported by a previous study [24]. The dysregulated lncRNAs and their potential targets involved in irisin-induced chondrogenic differentiation were analyzed.

2 Materials and methods

2.1 Retrieval and process of public data

The publicly available dataset GSE201594 contains RNA sequencing data that reflect the transcriptomic changes in human MSCs after irisin treatment (100 ng/ml) [24]. The dataset was selected in this study to identify key irisin-regulated lncRNAs in chondrogenic differentiation. Three repeated samples were used for each group [25]. The human MSCs were obtained from healthy volunteer donors. The SRA run files of RNA-seq samples were converted to fastq format with NCBI SRA Tool fastq-dump (v.2.8.0). The raw reads were trimmed to remove ambiguous reads (with N bases) using a FASTX-Toolkit (v.0.0.13; http://hannonlab.cshl.edu/fastx_toolkit/) and then filtered by removing the ending low-quality bases (quality score <20) and low-quality reads (30% bases with quality score <20). The quality of filtered reads was finally evaluated using the FastQC software (http://www.bioinformatics.babraham.ac.uk/projects/fastqc).

2.2 Reads alignment and differentially expressed genes (DEGs) analysis

The quality-filtered reads were aligned onto the human GRCH38 genome by HISAT2 (v.2.2.1) with no more than four mismatches. Uniquely aligned reads were screened for further analysis. The expression levels of genes were evaluated by fragments per kilobase of exon per million fragments mapped (FPKM) [26]. The DESeq2 (v.1.30.1) software [27] was used to identify DEGs with fold change (FC) ≥2 or ≤0.5 and p-value ≤0.01. We used a false discovery rate (FDR) <0.1 as a threshold value and obtained similar results. RNA-binding proteins (RBPs) on the list of 2,141 RBPs retrieved from previous reports [28,29,30,31] were filtered out from the DEGs.

2.3 lncRNA prediction

We combined the aligned results of RNA-seq data to assemble and predict novel transcripts using StringTie2 (v2.1.6) [32]. We eliminated the low abundant transcripts with FPKM <1. To identify lncRNAs from the assembled transcripts, we used 4 tools to predict the coding potential of transcripts: CPC2 (v2.0) [33], LGC (v1.0) [34], CNCI (v2.0) [35], and CPAT (v3.4.0) [36]. The noncoding transcripts longer than 200 bp were picked up as lncRNAs. Differentially expressed lncRNAs (DElncRNAs) were analyzed with the DESeq2 (v.1.30.1) software.

2.4 Correlation analysis

To analyze the potential regulatory functions of lncRNAs, correlation analysis was performed between all DElncRNAs and all DEGs. Pearson’s correlation coefficients (PCCs) between DElncRNAs and DEGs were calculated, and lncRNA-target relationship pairs satisfying the absolute value of PCC ≥0.6 and p-value ≤0.01 were identified.

2.5 Predict the relationship between DElncRNAs and microRNAs (miRNAs)

DElncRNAs can play a role as a sponge of miRNAs [37]. Here, we used two methods to predict the target relationship between miRNAs and DElncRNAs. One method is Miranda (https://anaconda.org/bioconda/miranda, v3.3) that predicts the miRNA complementary pairing with DElncRNA with a Miranda score >150. Another method is rnahybrid (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid/) that assumes miRNA–DElncRNA pairing with p-value ≤0.05. We took the overlapped pairs of the two methods as the final miRNA–DElncRNA target relationship. Finally, we analyzed the miRwalk database (http://mirwalk.umm.uni-heidelberg.de/) to find the differential targeting genes with default parameters.

2.6 Functional enrichment analysis

To explore the functions of identified gene sets, the enriched Gene Ontology (GO) and KEGG pathways for a given gene set were identified using KOBAS 2.0 [38]. We downloaded the KOBAS 2.0 pipeline to our local server and used the latest GO and KEGG databases to perform this analysis. Hypergeometric test and Benjamini–Hochberg FDR controlling procedure were used to define the enriched p-value and FDR of each pathway.

2.7 Statistical analysis

Principal component analysis (PCA) was performed by the R package factoextra (https://cloud.r-project.org/package=factoextra) to demonstrate the clustering of samples with the top two principal components. Unpaired Student’s t-test was used to analyze the statistical significance of two samples.

3 Results

3.1 lncRNAs are globally regulated by irisin in human MSCs

We downloaded the transcriptome sequencing data generated using human MSCs treated with irisin, which plays essential roles in glucose homeostasis [39] and chondrocyte biology [40]. The GSE201594 dataset containing three control and three irisin-treated samples [24] was analyzed. The published study using this dataset mainly analyzed the differentially expressed mRNA genes [24]. Here, we focused on the differentially expressed lncRNAs. There was a high overlap among the four methods that were used to predict lncRNAs (Figure 1a). The predicted lncRNAs had fewer exon number than mRNAs (Figure A1a). Transcript length distribution also revealed a shorter length for novel lncRNAs (Figure A2b).

Figure 1 Transcriptional analysis of differential expression lncRNAs in irisin-treated and control samples. (a) The overlap of predicted lncRNAs by the CPC, CNCI, CPAT, and LGC tools. (b) Volcano plot showing all DElncRNA between irisin and control samples with DESeq2. (c) PCA based on FPKM value of all DElncRNA. The ellipse for each group is the confidence ellipse. (d) The heatmap showing the expression level of DElncRNA. (e) The bar graph showing the number of DElncRNA FPKM ≥1, the abscissa is the number of samples, and the ordinate FPKM ≥1. (f) The heatmap diagram showing that the FPKM of DELncRNA of at least 4 samples is ≥1.
Figure 1

Transcriptional analysis of differential expression lncRNAs in irisin-treated and control samples. (a) The overlap of predicted lncRNAs by the CPC, CNCI, CPAT, and LGC tools. (b) Volcano plot showing all DElncRNA between irisin and control samples with DESeq2. (c) PCA based on FPKM value of all DElncRNA. The ellipse for each group is the confidence ellipse. (d) The heatmap showing the expression level of DElncRNA. (e) The bar graph showing the number of DElncRNA FPKM ≥1, the abscissa is the number of samples, and the ordinate FPKM ≥1. (f) The heatmap diagram showing that the FPKM of DELncRNA of at least 4 samples is ≥1.

We then performed DEG analysis of both mRNAs and lncRNAs between irisin and control. The down DEmRNAs (240) were similar to the up DEmRNAs (281) (Figure A1c). DElncRNAs analysis indicated 31 up DElncRNAs and 84 down DElncRNAs (Figure 1b and Table A1). PCA revealed the clear separation between irisin and control samples for DElncRNAs (Figure 1c). The hierarchical clustering heatmap also showed the consistently dysregulated expression level for DElncRNAs in irisin and control groups (Figure 1d). Most lncRNAs were lowly expressed in irisin-treated samples (Figure 1e). We then selected the top six down DElncRNAs and five up DElncRNAs for further analysis, including FLJ20021, LINC02574, LINC01618, RP11-160E2.6, AC112721.2, and RP11-138H8.8 (Figure 1f). The DEmRNAs were highly enriched in pathways associated with irisin functions (Figure A1d–e).

3.2 DElncRNAs are potential regulators for DEmRNAs in response to irisin

As regulatory RNAs, lncRNAs have ability to modulate gene expression in cis- or trans-manners. Co-expression analysis is a canonical method to explore the regulatory pairs between regulators and their targets [41]. We thus analyzed the significant correlation pairs between DElncRNAs and DEmRNAs (see details in materials and methods). The DElncRNAs detected were co-expressed with 496 DEmRNAs. In particular, the five lncRNAs were significantly correlated with more DEmRNAs, including XIST, PAX8-AS1, CASC15, LINC01618, and DLX6-AS1 (Figure 2a). We then explored the enriched functions of co-expressed DEmRNAs using GO and KEGG databases. The top 10 enriched GO biological processes contained skeletal system development, extracellular matrix (ECM) organization, cell adhesion, skeletal system morphogenesis, and response to nicotine (Figure 2b). Meanwhile, endochondral ossification and bone mineralization were also enriched (Figure 2b). The KEGG pathway analysis revealed several enriched diseases and pathways, including rheumatoid arthritis (Figure A2a). To better display the co-expression pairs between DElncRNAs and DEmRNAs, we performed network presentation for DElncRNAs, DEmRNAs, and their enriched GO BP pathways to demonstrate the co-expression network and the co-expressed strength (Figure 2c). These results indicate that DElncRNAs have the potential to broadly regulate the expression of DEmRNAs in response to irisin in human MSCs.

Figure 2 Analysis of interaction between DElncRNAs and DEmRNAs in irisin vs control samples. (a) Scatter plot showing DElncRNAs by irisin compared with control samples and its number of co-expressed DEmRNAs. Red points denote up-regulated lncRNAs involved in co-expression pairs and blue points denote down-regulated lncRNAs. Cutoffs of p value <0.01 and Pearson coefficient >0.6 were applied to identify the co-expression pairs. (b) Bar plot exhibited the most enriched GO biological process results of DEmRNAs co-expressed with DELncRNAs. (c) Network diagram showing top 10 GO biological process results of DEmRNAs regulated by DElncRNAs.
Figure 2

Analysis of interaction between DElncRNAs and DEmRNAs in irisin vs control samples. (a) Scatter plot showing DElncRNAs by irisin compared with control samples and its number of co-expressed DEmRNAs. Red points denote up-regulated lncRNAs involved in co-expression pairs and blue points denote down-regulated lncRNAs. Cutoffs of p value <0.01 and Pearson coefficient >0.6 were applied to identify the co-expression pairs. (b) Bar plot exhibited the most enriched GO biological process results of DEmRNAs co-expressed with DELncRNAs. (c) Network diagram showing top 10 GO biological process results of DEmRNAs regulated by DElncRNAs.

3.3 DElncRNAs act as ceRNAs to regulate expression of RBPs

One canonical functional manner for lncRNAs is to act as ceRNAs with miRNAs, thus indirectly modulating mRNA expression [42]. After predicting the targets of miRNAs using base-pair methods, we overlapped the miRNA targets and DEmRNAs, and identified 340 overlapped mRNAs, occupying 65% of all DEmRNAs (Figure 3a). Functional enrichment analysis of these overlapped DEmRNAs revealed similar enriched pathways with all DEmRNAs (Figure 3b and Figure A3a), suggesting that DEmRNAs could be broadly regulated by miRNAs. As one large family of proteins, RBPs play essential roles in transcriptional and post-transcriptional regulation [30]. We found that 13 out of 15 overlapped RBPs were miRNA targets (Figure 3a), indicating that RBPs were more likely to be regulated by miRNAs. Among the 13 RBPs, 8 were up-regulated and 5 down-regulated (Figure A3b). We then plotted the regulatory network for DElncRNAs–miRNAs–DERBPs. Several DERBPs, including RBM48, SORBS1, PCDHGA9, RPP25L, and ROR2, were highly regulated by more miRNAs than other DERBPs (Figure 3c). Eight DElncRNAs were involved in this regulatory network, two up-regulated and six down-regulated (Figure 3d).

Figure 3 Co-expression network of DElncRNAs–miRNAs–DERBPs between irisin vs control. (a) Venn diagram showing the overlap of microRNA-differential target genes and RBPs. (b) Bar plot showing the most enriched GO biological processes for differential miRNA target genes. (c) Network diagram showing the DElncRNA–microRNA–DERBPs. (d) Boxplot showing FPKM of eight DElncRNA. *p  <  0.05, **p  <  0.01, ***p  <  0.001.
Figure 3

Co-expression network of DElncRNAs–miRNAs–DERBPs between irisin vs control. (a) Venn diagram showing the overlap of microRNA-differential target genes and RBPs. (b) Bar plot showing the most enriched GO biological processes for differential miRNA target genes. (c) Network diagram showing the DElncRNA–microRNA–DERBPs. (d) Boxplot showing FPKM of eight DElncRNA. *p  <  0.05, **p  <  0.01, ***p  <  0.001.

4 Discussion

Dedifferentiation or degradation of cartilage leads to multiple diseases including OA [3]. Identification of the key factors that can prevent chondrocyte dedifferentiation is of significance in cartilage repair. Irisin plays an important role in a wide range of diseases such as OA, sarcopenia, metabolic diseases, and neurological diseases [43,44]. In vitro studies have indicated irisin as a positive regulator of chondrogenic differentiation [18,19,20]. In this study, we demonstrate the alteration of lncRNA expression profile by irisin in human MSCs and describe the potential lncRNAs and downstream targets involved in chondrogenic differentiation. Our work sheds light on the regulation of lncRNA mediators by irisin and reveals novel regulatory lncRNAs during chondrogenic differentiation.

Previous studies have demonstrated that irisin treatment elicits proteomic and transcriptomic changes in multiple types of cells [21,22,23]. For instance, Dehghan et al. [22] reported that irisin injection alters dozens of proteins in murine brain cells. Mathias et al. [23] demonstrated that irisin treatment modulates a lot of genes related to the purinergic signaling in differentiated human adipocytes. Consistently, our study indicates that irisin treatment causes a global alteration of lncRNAs in human MSCs. Among the irisin-regulated lncRNAs, several ones exhibit the capacity to modulate the progression of malignant diseases. For instance, the lncRNA CASC15 promotes the proliferation and invasion of lung cancer cells via the miR-766-5p/KLK12 axis [45]. Another lncRNA, PAX8-AS1, can inhibit the proliferation and induce apoptosis in papillary thyroid carcinoma cells [46]. However, few lncRNAs have been investigated in the course of chondrogenic differentiation. Intriguingly, we identified two irisin-regulated lncRNAs, i.e., XIST and DLX6-AS1, which can regulate osteogenic differentiation [47,48]. Liu et al. [47] reported that DLX6-AS1 is upregulated in dental pulp cells by BMP9 and promotes osteogenic differentiation. Zheng et al. [48] reported that knockdown of XIST blocks the osteogenic differentiation of human bone marrow MSCs. Moreover, XIST can modulate the proliferation and survival of chondrocytes and thus contribute to the progression of OA [49,50]. These studies, combined with our findings, suggest the possibility that irisin may promote chondrogenic differentiation through modulation of key lncRNAs including XIST and DLX6-AS1.

Functional enrichment analysis reveals that the irisin-regulated lncRNAs are associated with target mRNAs involved in ECM organization, cell adhesion, bone mineralization, response to hypoxia, and inflammation. Hypoxia-inducible factor 1α regulates chondrogenesis by transactivating the expression of SOX9 [51]. The lncRNA XIST can promote SOX9 expression through the IL-6/STAT3 signaling pathway [52]. In addition, chondrogenic differentiation is regulated by specific ECM components [53]. Notably, XIST shows the ability to promote ECM degradation [54]. These findings suggest that irisin-regulated lncRNAs may modulate key transcription factors such as SOX9 to affect ECM deposition and chondrogenic differentiation.

In addition to the direct modulation of target genes, lncRNAs act as ceRNAs to regulate gene expression via an indirect manner. RBPs have been reported to regulate anabolic and catabolic gene expression in chondrocytes [55]. In this study, we identified seven lncRNAs that can regulate RBPs by sponging miRNAs. The lncRNAs LOC100126784 and POM121L9P are the upstream ceRNAs of miR-503-5p and promote osteogenic differentiation of bone MSCs by regulating SORBS1 expression [56]. These studies provide another possible mechanism by which lncRNAs regulate chondrogenic differentiation induced by irisin.

Several limitations of this study should be noted. First, the functions of the irisin-regulated lncRNAs in chondrogenic differentiation have not been determined. The lncRNA XIST is a promising one mediating the activity of irisin in chondrogenic differentiation. However, there is a lack of direct evidence for this speculation. Second, the regulation of lncRNA expression profile by irisin needs to be validated in clinical samples.

In summary, irisin treatment leads to a global alteration of lncRNAs in human MSCs, which modulate a large number of target genes through direct or indirect mechanisms. Irisin-regulated lncRNAs may serve as potential therapeutic targets in the prevention and treatment of cartilage injury. Further work should be conducted to fully characterize the key irisin-regulated lncRNAs in chondrogenic differentiation.

Acknowledgments

We thanked the technical support of Xiaoyi Hu.

  1. Funding information: This study was supported by the National Natural Science Foundation of China (81670917); Funds for the Second Affiliated Hospital of Xi’an Jiaotong University (2020YJ-041); and Funds for Shaaxi Province Zhong Dian Yan Fa Project (2022SF-387).

  2. Author contributions: YJ Chen and ZH Wang designed the study. RX Guo, WY Kou, and L Yang performed the software analysis. WQ Sha and CY Li performed statistical analysis. YJ Chen, YF Zhang, and JJ Zhao prepared the manuscript.

  3. Conflict of interest: All authors have contributed significantly and are in agreement with the content of the manuscript.

  4. Data availability statement: The datasets generated and/or analyzed during the current study are available in the manuscript. All data generated or analyzed during this study can be easily acquired from the corresponding author upon reasonable request.

Appendix

Figure A1 Transcriptional analysis of differential expression lncRNA in irisin and control. (a) Distribution of exon count of known lncRNA, novel lncRNA, and protein coding RNA. (b) Density of the length distribution of known lncRNA, novel lncRNA, and protein coding RNA. The length density distribution was generated by density function. (c) Volcano plot showing all DEmRNA between irisin and control samples with DESeq2. P value < 0.01 and FC (fold change) ≥ 2 or ≤ 0.5. (d) Bar plot exhibiting the most enriched GO biological process results of the up-regulated DEGs. (e) Bar plot exhibiting the most enriched GO biological process results of the down-regulated DEGs.
Figure A1

Transcriptional analysis of differential expression lncRNA in irisin and control. (a) Distribution of exon count of known lncRNA, novel lncRNA, and protein coding RNA. (b) Density of the length distribution of known lncRNA, novel lncRNA, and protein coding RNA. The length density distribution was generated by density function. (c) Volcano plot showing all DEmRNA between irisin and control samples with DESeq2. P value < 0.01 and FC (fold change) ≥ 2 or ≤ 0.5. (d) Bar plot exhibiting the most enriched GO biological process results of the up-regulated DEGs. (e) Bar plot exhibiting the most enriched GO biological process results of the down-regulated DEGs.

Figure A2 Analysis of interaction between DElncRNA and DEmRNA in irisin and control. (a) Bar plot showing the top 10 enriched KEGG pathways of DEmRNA co expressed by DELncRNA.
Figure A2

Analysis of interaction between DElncRNA and DEmRNA in irisin and control. (a) Bar plot showing the top 10 enriched KEGG pathways of DEmRNA co expressed by DELncRNA.

Figure A3 Co-expression network of DElncRNA-microRNA-DERBPs in irisin and control. (a) Bar plot showing the top 10 enriched KEGG pathways of differential miRNA target genes. (b) The heatmap diagram showing the expression profile of DERBPs in Figure 3A.
Figure A3

Co-expression network of DElncRNA-microRNA-DERBPs in irisin and control. (a) Bar plot showing the top 10 enriched KEGG pathways of differential miRNA target genes. (b) The heatmap diagram showing the expression profile of DERBPs in Figure 3A.

References

[1] Le H, Xu W, Zhuang X, Chang F, Wang Y, Ding J. Mesenchymal stem cells for cartilage regeneration. J Tissue Eng. 2020;11:2041731420943839.10.1177/2041731420943839Search in Google Scholar PubMed PubMed Central

[2] Kuo CK, Li WJ, Mauck RL, Tuan RS. Cartilage tissue engineering: its potential and uses. Curr Opin Rheumatol. 2006;18(1):64–73.10.1097/01.bor.0000198005.88568.dfSearch in Google Scholar PubMed

[3] Ma B, Leijten JC, Wu L, Kip M, van Blitterswijk CA, Post JN, et al. Gene expression profiling of dedifferentiated human articular chondrocytes in monolayer culture. Osteoarthr Cartilage. 2013;21(4):599–603.10.1016/j.joca.2013.01.014Search in Google Scholar PubMed

[4] Wang ZH, Yang ZQ, He XJ, Wang L, Li LX, Tu JB. Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytes in vitro. Acta Pharmacol Sin. 2008;29(10):1215–26.10.1111/j.1745-7254.2008.00856.xSearch in Google Scholar PubMed

[5] Charlier E, Deroyer C, Ciregia F, Malaise O, Neuville S, Plener Z, et al. Chondrocyte dedifferentiation and osteoarthritis (OA). Biochem Pharmacol. 2019;165:49–65.10.1016/j.bcp.2019.02.036Search in Google Scholar PubMed

[6] Haseeb A, Kc R, Angelozzi M, de Charleroy C, Rux D, Tower RJ, et al. SOX9 keeps growth plates and articular cartilage healthy by inhibiting chondrocyte dedifferentiation/osteoblastic redifferentiation. Proc Natl Acad Sci U S A. 2021;118(8):e2019152118.10.1073/pnas.2019152118Search in Google Scholar PubMed PubMed Central

[7] Md Nazir N, Zulkifly AH, Khalid KA, Zainol I, Zamli Z, Sha’ban M. Matrix production in chondrocytes transfected with sex determining region Y-Box 9 and telomerase reverse transcriptase genes: an in vitro evaluation from monolayer culture to three-dimensional culture. Tissue Eng Regener Med. 2019;16(3):285–99.10.1007/s13770-019-00191-1Search in Google Scholar PubMed PubMed Central

[8] Gurusinghe S, Young P, Michelsen J, Strappe P. Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors. Biotechnol Lett. 2015;37(7):1495–504.10.1007/s10529-015-1805-5Search in Google Scholar PubMed

[9] Wang L, Li Z, Li Z, Yu B, Wang Y. Long noncoding RNAs expression signatures in chondrogenic differentiation of human bone marrow mesenchymal stem cells. Biochem Biophys Res Commun. 2015;456(1):459–64.10.1016/j.bbrc.2014.11.106Search in Google Scholar PubMed

[10] Herman AB, Tsitsipatis D, Gorospe M. Integrated lncRNA function upon genomic and epigenomic regulation. Mol Cell. 2022;82(12):2252–66.10.1016/j.molcel.2022.05.027Search in Google Scholar PubMed PubMed Central

[11] de Goede OM, Nachun DC, Ferraro NM, Gloudemans MJ, Rao AS, Smail C, et al. Population-scale tissue transcriptomics maps long non-coding RNAs to complex disease. Cell. 2021;184(10):2633–48.10.1016/j.cell.2021.03.050Search in Google Scholar PubMed PubMed Central

[12] Sarropoulos I, Marin R, Cardoso-Moreira M, Kaessmann H. Developmental dynamics of lncRNAs across mammalian organs and species. Nature. 2019;571(7766):510–4.10.1038/s41586-019-1341-xSearch in Google Scholar PubMed PubMed Central

[13] Barter MJ, Gomez R, Hyatt S, Cheung K, Skelton AJ, Xu Y, et al. The long non-coding RNA ROCR contributes to SOX9 expression and chondrogenic differentiation of human mesenchymal stem cells. Development. 2017;144(24):4510–21.10.1242/dev.152504Search in Google Scholar PubMed PubMed Central

[14] You D, Yang C, Huang J, Gong H, Yan M, Ni J. Long non-coding RNA MEG3 inhibits chondrogenic differentiation of synovium-derived mesenchymal stem cells by epigenetically inhibiting TRIB2 via methyltransferase EZH2. Cell Signal. 2019;63:109379.10.1016/j.cellsig.2019.109379Search in Google Scholar PubMed

[15] Zhang L, Yang C, Chen S, Wang G, Shi B, Tao X, et al. Long noncoding RNA DANCR is a positive regulator of proliferation and chondrogenic differentiation in human synovium-derived stem cells. DNA Cell Biol. 2017;36(2):136–42.10.1089/dna.2016.3544Search in Google Scholar PubMed

[16] Huang MJ, Zhao JY, Xu JJ, Li J, Zhuang YF, Zhang XL. lncRNA ADAMTS9-AS2 controls human mesenchymal stem cell chondrogenic differentiation and functions as a ceRNA. Mol Ther Nucleic Acids. 2019;18:533–45.10.1016/j.omtn.2019.08.027Search in Google Scholar PubMed PubMed Central

[17] Zhou X, Xu W, Wang Y, Zhang H, Zhang L, Li C, et al. LncRNA DNM3OS regulates GREM2 via miR-127-5p to suppress early chondrogenic differentiation of rat mesenchymal stem cells under hypoxic conditions. Cell Mol Biol Lett. 2021;26(1):22.10.1186/s11658-021-00269-6Search in Google Scholar PubMed PubMed Central

[18] Vadala G, Di Giacomo G, Ambrosio L, Cannata F, Cicione C, Papalia R, et al. Irisin recovers osteoarthritic chondrocytes in vitro. Cells. 2020;9(6):1478.10.3390/cells9061478Search in Google Scholar PubMed PubMed Central

[19] Li X, Zhu X, Wu H, Van Dyke TE, Xu X, Morgan EF, et al. Roles and mechanisms of irisin in attenuating pathological features of osteoarthritis. Front Cell Dev Biol. 2021;9:703670.10.3389/fcell.2021.703670Search in Google Scholar PubMed PubMed Central

[20] Chen X, Sun K, Zhao S, Geng T, Fan X, Sun S, et al. Irisin promotes osteogenic differentiation of bone marrow mesenchymal stem cells by activating autophagy via the Wnt/beta-catenin signal pathway. Cytokine. 2020;136:155292.10.1016/j.cyto.2020.155292Search in Google Scholar PubMed

[21] Momenzadeh S, Zamani S, Pourteymourfard-Tabrizi Z, Barreiro C, Jami MS. Muscles proteome analysis; irisin administration mimics some molecular effects of exercise in quadriceps muscle. Biochimie. 2021;189:144–57.10.1016/j.biochi.2021.06.016Search in Google Scholar PubMed

[22] Dehghan F, Zamani S, Barreiro C, Jami MS. Irisin injection mimics exercise effects on the brain proteome. Eur J Neurosci. 2021;54(10):7422–41.10.1111/ejn.15493Search in Google Scholar PubMed

[23] Mathias LS, Herman-de-Sousa C, Cury SS, Nogueira CR, Correia-de-Sá P, de Oliveira M. RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes. Biochim Biophys Acta Mol Cell Biol Lipids. 2023;1868(4):159276.10.1016/j.bbalip.2022.159276Search in Google Scholar PubMed

[24] Chen T, Peng Y, Hu W, Shi H, Li P, Que Y, et al. Irisin enhances chondrogenic differentiation of human mesenchymal stem cells via Rap1/PI3K/AKT axis. Stem Cell Res Ther. 2022;13(1):392.10.1186/s13287-022-03092-8Search in Google Scholar PubMed PubMed Central

[25] Adam Z. Samples in many cell-based experiments are matched/paired but taking this into account does not always increase power of statistical tests for differences in means. Mol Biol Cell. 2024;35(1):br1. 10.1091/mbc. E23-05-0159.Search in Google Scholar

[26] Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28(5):511–5.10.1038/nbt.1621Search in Google Scholar PubMed PubMed Central

[27] Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15(12):550.10.1186/s13059-014-0550-8Search in Google Scholar PubMed PubMed Central

[28] Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, et al. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell. 2012;149(6):1393–406.10.1016/j.cell.2012.04.031Search in Google Scholar PubMed

[29] Castello A, Fischer B, Frese CK, Horos R, Alleaume AM, Foehr S, et al. Comprehensive identification of RNA-binding domains in human cells. Mol Cell. 2016;63(4):696–710.10.1016/j.molcel.2016.06.029Search in Google Scholar PubMed PubMed Central

[30] Gerstberger S, Hafner M, Tuschl T. A census of human RNA-binding proteins. Nat Rev Genet. 2014;15(12):829–45.10.1038/nrg3813Search in Google Scholar PubMed PubMed Central

[31] Hentze MW, Castello A, Schwarzl T, Preiss T. A brave new world of RNA-binding proteins. Nat Rev Mol Cell Biol. 2018;19(5):327–41.10.1038/nrm.2017.130Search in Google Scholar PubMed

[32] Kovaka S, Zimin AV, Pertea GM, Razaghi R, Salzberg SL, Pertea M. Transcriptome assembly from long-read RNA-seq alignments with StringTie2. Genome Biol. 2019;20(1):278.10.1186/s13059-019-1910-1Search in Google Scholar PubMed PubMed Central

[33] Kong L, Zhang Y, Ye ZQ, Liu XQ, Gao G. CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic Acids Res. 2007;35:(Web Server issue) W345–9.10.1093/nar/gkm391Search in Google Scholar PubMed PubMed Central

[34] Wang G, Yin H, Li B, Yu C, Wang F, Xu X, et al. Characterization and identification of long non-coding RNAs based on feature relationship. Bioinformatics. 2019;35(17):2949–56.10.1093/bioinformatics/btz008Search in Google Scholar PubMed

[35] Sun L, Luo H, Bu D, Zhao G, Yu K, Zhang C, et al. Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts. Nucleic Acids Res. 2013;41(17):e166.10.1093/nar/gkt646Search in Google Scholar PubMed PubMed Central

[36] Wang L, Park HJ, Dasari S, Wang S, Kocher JP, Li W. CPAT: Coding-Potential Assessment Tool using an alignment-free logistic regression model. Nucleic Acids Res. 2013;41(6):e74.10.1093/nar/gkt006Search in Google Scholar PubMed PubMed Central

[37] Militello G, Weirick T, John D, Doring C, Dimmeler S, Uchida S. Screening and validation of lncRNAs and circRNAs as miRNA sponges. Briefings Bioinform. 2017;18(5):780–88.10.1093/bib/bbw053Search in Google Scholar PubMed

[38] Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S, et al. KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res. 2011;39(Web Server issue):W316–322.10.1093/nar/gkr483Search in Google Scholar PubMed PubMed Central

[39] Perakakis N, Triantafyllou GA, Fernandez-Real JM, Huh JY, Park KH, Seufert J, et al. Physiology and role of irisin in glucose homeostasis. Nat Rev Endocrinol. 2017;13(6):324–37.10.1038/nrendo.2016.221Search in Google Scholar PubMed PubMed Central

[40] Wang FS, Kuo CW, Ko JY, Chen YS, Wang SY, Ke HJ, et al. Irisin mitigates oxidative stress, chondrocyte dysfunction and osteoarthritis development through regulating mitochondrial integrity and autophagy. Antioxid (Basel). 2020;9(9):810.10.3390/antiox9090810Search in Google Scholar PubMed PubMed Central

[41] Stuart JM, Segal E, Koller D, Kim SK. A gene-coexpression network for global discovery of conserved genetic modules. Science. 2003;302(5643):249–55.10.1126/science.1087447Search in Google Scholar PubMed

[42] Tay Y, Rinn J, Pandolfi PP. The multilayered complexity of ceRNA crosstalk and competition. Nature. 2014;505(7483):344–52.10.1038/nature12986Search in Google Scholar PubMed PubMed Central

[43] Zhang Y, Zhang X, Lin S. Irisin: A bridge between exercise and neurological diseases. Heliyon. 2022;8(12):e12352.10.1016/j.heliyon.2022.e12352Search in Google Scholar PubMed PubMed Central

[44] Guo M, Yao J, Li J, Zhang J, Wang D, Zuo H, et al. Irisin ameliorates age-associated sarcopenia and metabolic dysfunction. J Cachexia Sarcopenia Muscle. 2023;14(1):391–405.10.1002/jcsm.13141Search in Google Scholar PubMed PubMed Central

[45] Bai Y, Zhang G, Cheng R, Yang R, Chu H. CASC15 contributes to proliferation and invasion through regulating miR-766-5p/KLK12 axis in lung cancer. Cell Cycle. 2019;18(18):2323–31.10.1080/15384101.2019.1646562Search in Google Scholar PubMed PubMed Central

[46] Zhou P, Xu T, Hu H, Hua F. Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis. Int J Endocrinol. 2021;2021:5499963.10.1155/2021/5499963Search in Google Scholar PubMed PubMed Central

[47] Liu L, Fang T, Miao C, Li X, Zeng Y, Wang T, et al. DLX6-AS1 regulates odonto/osteogenic differentiation in dental pulp cells under the control of BMP9 via the miR-128-3p/MAPK14 axis: A laboratory investigation. Int Endod J. 2024;57(11):1623–38. 10.1111/iej.14120 Search in Google Scholar PubMed

[48] Zheng C, Bai C, Sun Q, Zhang F, Yu Q, Zhao X, et al. Long noncoding RNA XIST regulates osteogenic differentiation of human bone marrow mesenchymal stem cells by targeting miR-9-5p. Mech Dev. 2020;162:103612.10.1016/j.mod.2020.103612Search in Google Scholar PubMed

[49] Liu Y, Liu K, Tang C, Shi Z, Jing K, Zheng J. Long non-coding RNA XIST contributes to osteoarthritis progression via miR-149-5p/DNMT3A axis. Biomed Pharmacother. 2020;128:110349.10.1016/j.biopha.2020.110349Search in Google Scholar PubMed

[50] Chen H, Yang S, Shao R. Long non-coding XIST raises methylation of TIMP-3 promoter to regulate collagen degradation in osteoarthritic chondrocytes after tibial plateau fracture. Arthritis Res Ther. 2019;21(1):271.10.1186/s13075-019-2033-5Search in Google Scholar PubMed PubMed Central

[51] Amarilio R, Viukov SV, Sharir A, Eshkar-Oren I, Johnson RS, Zelzer E. HIF1alpha regulation of Sox9 is necessary to maintain differentiation of hypoxic prechondrogenic cells during early skeletogenesis. Development. 2007;134(21):3917–28.10.1242/dev.008441Search in Google Scholar PubMed

[52] Ma Y, Zhu Y, Shang L, Qiu Y, Shen N, Wang J, et al. LncRNA XIST regulates breast cancer stem cells by activating proinflammatory IL-6/STAT3 signaling. Oncogene. 2023;42(18):1419–37.10.1038/s41388-023-02652-3Search in Google Scholar PubMed PubMed Central

[53] Maldonado M, Nam J. The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis. Biomed Res Int. 2013;2013:284873.10.1155/2013/284873Search in Google Scholar PubMed PubMed Central

[54] Wang T, Liu Y, Wang Y, Huang X, Zhao W, Zhao Z. Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis. Int J Mol Med. 2019;44(2):630–42.10.3892/ijmm.2019.4240Search in Google Scholar PubMed PubMed Central

[55] McDermott BT, Ellis S, Bou-Gharios G, Clegg PD, Tew SR. RNA binding proteins regulate anabolic and catabolic gene expression in chondrocytes. Osteoarthr Cartilage. 2016;24(7):1263–73.10.1016/j.joca.2016.01.988Search in Google Scholar PubMed PubMed Central

[56] Xu Y, Xin R, Sun H, Long D, Li Z, Liao H, et al. Long non-coding RNAs LOC100126784 and POM121L9P derived from bone marrow mesenchymal stem cells enhance osteogenic differentiation via the miR-503-5p/SORBS1 Axis. Front Cell Dev Biol. 2021;9:723759.10.3389/fcell.2021.723759Search in Google Scholar PubMed PubMed Central

Received: 2024-04-08
Revised: 2024-09-20
Accepted: 2024-10-01
Published Online: 2024-11-15

© 2024 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Articles in the same Issue

  1. Research Articles
  2. EDNRB inhibits the growth and migration of prostate cancer cells by activating the cGMP-PKG pathway
  3. STK11 (LKB1) mutation suppresses ferroptosis in lung adenocarcinoma by facilitating monounsaturated fatty acid synthesis
  4. Association of SOX6 gene polymorphisms with Kashin-Beck disease risk in the Chinese Han population
  5. The pyroptosis-related signature predicts prognosis and influences the tumor immune microenvironment in dedifferentiated liposarcoma
  6. METTL3 attenuates ferroptosis sensitivity in lung cancer via modulating TFRC
  7. Identification and validation of molecular subtypes and prognostic signature for stage I and stage II gastric cancer based on neutrophil extracellular traps
  8. Novel lumbar plexus block versus femoral nerve block for analgesia and motor recovery after total knee arthroplasty
  9. Correlation between ABCB1 and OLIG2 polymorphisms and the severity and prognosis of patients with cerebral infarction
  10. Study on the radiotherapy effect and serum neutral granulocyte lymphocyte ratio and inflammatory factor expression of nasopharyngeal carcinoma
  11. Transcriptome analysis of effects of Tecrl deficiency on cardiometabolic and calcium regulation in cardiac tissue
  12. Aflatoxin B1 induces infertility, fetal deformities, and potential therapies
  13. Serum levels of HMW adiponectin and its receptors are associated with cytokine levels and clinical characteristics in chronic obstructive pulmonary disease
  14. METTL3-mediated methylation of CYP2C19 mRNA may aggravate clopidogrel resistance in ischemic stroke patients
  15. Understand how machine learning impact lung cancer research from 2010 to 2021: A bibliometric analysis
  16. Pressure ulcers in German hospitals: Analysis of reimbursement and length of stay
  17. Metformin plus L-carnitine enhances brown/beige adipose tissue activity via Nrf2/HO-1 signaling to reduce lipid accumulation and inflammation in murine obesity
  18. Downregulation of carbonic anhydrase IX expression in mouse xenograft nasopharyngeal carcinoma model via doxorubicin nanobubble combined with ultrasound
  19. Feasibility of 3-dimensional printed models in simulated training and teaching of transcatheter aortic valve replacement
  20. miR-335-3p improves type II diabetes mellitus by IGF-1 regulating macrophage polarization
  21. The analyses of human MCPH1 DNA repair machinery and genetic variations
  22. Activation of Piezo1 increases the sensitivity of breast cancer to hyperthermia therapy
  23. Comprehensive analysis based on the disulfidptosis-related genes identifies hub genes and immune infiltration for pancreatic adenocarcinoma
  24. Changes of serum CA125 and PGE2 before and after high-intensity focused ultrasound combined with GnRH-a in treatment of patients with adenomyosis
  25. The clinical value of the hepatic venous pressure gradient in patients undergoing hepatic resection for hepatocellular carcinoma with or without liver cirrhosis
  26. Development and validation of a novel model to predict pulmonary embolism in cardiology suspected patients: A 10-year retrospective analysis
  27. Downregulation of lncRNA XLOC_032768 in diabetic patients predicts the occurrence of diabetic nephropathy
  28. Circ_0051428 targeting miR-885-3p/MMP2 axis enhances the malignancy of cervical cancer
  29. Effectiveness of ginkgo diterpene lactone meglumine on cognitive function in patients with acute ischemic stroke
  30. The construction of a novel prognostic prediction model for glioma based on GWAS-identified prognostic-related risk loci
  31. Evaluating the impact of childhood BMI on the risk of coronavirus disease 2019: A Mendelian randomization study
  32. Lactate dehydrogenase to albumin ratio is associated with in-hospital mortality in patients with acute heart failure: Data from the MIMIC-III database
  33. CD36-mediated podocyte lipotoxicity promotes foot process effacement
  34. Efficacy of etonogestrel subcutaneous implants versus the levonorgestrel-releasing intrauterine system in the conservative treatment of adenomyosis
  35. FLRT2 mediates chondrogenesis of nasal septal cartilage and mandibular condyle cartilage
  36. Challenges in treating primary immune thrombocytopenia patients undergoing COVID-19 vaccination: A retrospective study
  37. Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway
  38. Phospholipid transfer protein ameliorates sepsis-induced cardiac dysfunction through NLRP3 inflammasome inhibition
  39. Postoperative cognitive dysfunction in elderly patients with colorectal cancer: A randomized controlled study comparing goal-directed and conventional fluid therapy
  40. Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction
  41. High SEC61A1 expression predicts poor outcome of acute myeloid leukemia
  42. Comparison of polymerase chain reaction and next-generation sequencing with conventional urine culture for the diagnosis of urinary tract infections: A meta-analysis
  43. Secreted frizzled-related protein 5 protects against renal fibrosis by inhibiting Wnt/β-catenin pathway
  44. Pan-cancer and single-cell analysis of actin cytoskeleton genes related to disulfidptosis
  45. Overexpression of miR-532-5p restrains oxidative stress response of chondrocytes in nontraumatic osteonecrosis of the femoral head by inhibiting ABL1
  46. Autologous liver transplantation for unresectable hepatobiliary malignancies in enhanced recovery after surgery model
  47. Clinical analysis of incomplete rupture of the uterus secondary to previous cesarean section
  48. Abnormal sleep duration is associated with sarcopenia in older Chinese people: A large retrospective cross-sectional study
  49. No genetic causality between obesity and benign paroxysmal vertigo: A two-sample Mendelian randomization study
  50. Identification and validation of autophagy-related genes in SSc
  51. Long non-coding RNA SRA1 suppresses radiotherapy resistance in esophageal squamous cell carcinoma by modulating glycolytic reprogramming
  52. Evaluation of quality of life in patients with schizophrenia: An inpatient social welfare institution-based cross-sectional study
  53. The possible role of oxidative stress marker glutathione in the assessment of cognitive impairment in multiple sclerosis
  54. Compilation of a self-management assessment scale for postoperative patients with aortic dissection
  55. Left atrial appendage closure in conjunction with radiofrequency ablation: Effects on left atrial functioning in patients with paroxysmal atrial fibrillation
  56. Effect of anterior femoral cortical notch grade on postoperative function and complications during TKA surgery: A multicenter, retrospective study
  57. Clinical characteristics and assessment of risk factors in patients with influenza A-induced severe pneumonia after the prevalence of SARS-CoV-2
  58. Analgesia nociception index is an indicator of laparoscopic trocar insertion-induced transient nociceptive stimuli
  59. High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression
  60. Factors influencing cardiovascular system-related post-COVID-19 sequelae: A single-center cohort study
  61. HOXD10 regulates intestinal permeability and inhibits inflammation of dextran sulfate sodium-induced ulcerative colitis through the inactivation of the Rho/ROCK/MMPs axis
  62. Mesenchymal stem cell-derived exosomal miR-26a induces ferroptosis, suppresses hepatic stellate cell activation, and ameliorates liver fibrosis by modulating SLC7A11
  63. Endovascular thrombectomy versus intravenous thrombolysis for primary distal, medium vessel occlusion in acute ischemic stroke
  64. ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis
  65. Prognostic value of EIF5A2 in solid tumors: A meta-analysis and bioinformatics analysis
  66. The role of enhanced expression of Cx43 in patients with ulcerative colitis
  67. Choosing a COVID-19 vaccination site might be driven by anxiety and body vigilance
  68. Role of ICAM-1 in triple-negative breast cancer
  69. Cost-effectiveness of ambroxol in the treatment of Gaucher disease type 2
  70. HLA-DRB5 promotes immune thrombocytopenia via activating CD8+ T cells
  71. Efficacy and factors of myofascial release therapy combined with electrical and magnetic stimulation in the treatment of chronic pelvic pain syndrome
  72. Efficacy of tacrolimus monotherapy in primary membranous nephropathy
  73. Mechanisms of Tripterygium wilfordii Hook F on treating rheumatoid arthritis explored by network pharmacology analysis and molecular docking
  74. FBXO45 levels regulated ferroptosis renal tubular epithelial cells in a model of diabetic nephropathy by PLK1
  75. Optimizing anesthesia strategies to NSCLC patients in VATS procedures: Insights from drug requirements and patient recovery patterns
  76. Alpha-lipoic acid upregulates the PPARγ/NRF2/GPX4 signal pathway to inhibit ferroptosis in the pathogenesis of unexplained recurrent pregnancy loss
  77. Correlation between fat-soluble vitamin levels and inflammatory factors in paediatric community-acquired pneumonia: A prospective study
  78. CD1d affects the proliferation, migration, and apoptosis of human papillary thyroid carcinoma TPC-1 cells via regulating MAPK/NF-κB signaling pathway
  79. miR-let-7a inhibits sympathetic nerve remodeling after myocardial infarction by downregulating the expression of nerve growth factor
  80. Immune response analysis of solid organ transplantation recipients inoculated with inactivated COVID-19 vaccine: A retrospective analysis
  81. The H2Valdien derivatives regulate the epithelial–mesenchymal transition of hepatoma carcinoma cells through the Hedgehog signaling pathway
  82. Clinical efficacy of dexamethasone combined with isoniazid in the treatment of tuberculous meningitis and its effect on peripheral blood T cell subsets
  83. Comparison of short-segment and long-segment fixation in treatment of degenerative scoliosis and analysis of factors associated with adjacent spondylolisthesis
  84. Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathway
  85. Methylation regulation for FUNDC1 stability in childhood leukemia was up-regulated and facilitates metastasis and reduces ferroptosis of leukemia through mitochondrial damage by FBXL2
  86. Correlation of single-fiber electromyography studies and functional status in patients with amyotrophic lateral sclerosis
  87. Risk factors of postoperative airway obstruction complications in children with oral floor mass
  88. Expression levels and clinical significance of serum miR-19a/CCL20 in patients with acute cerebral infarction
  89. Physical activity and mental health trends in Korean adolescents: Analyzing the impact of the COVID-19 pandemic from 2018 to 2022
  90. Evaluating anemia in HIV-infected patients using chest CT
  91. Ponticulus posticus and skeletal malocclusion: A pilot study in a Southern Italian pre-orthodontic court
  92. Causal association of circulating immune cells and lymphoma: A Mendelian randomization study
  93. Assessment of the renal function and fibrosis indexes of conventional western medicine with Chinese medicine for dredging collaterals on treating renal fibrosis: A systematic review and meta-analysis
  94. Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer
  95. New target-HMGCR inhibitors for the treatment of primary sclerosing cholangitis: A drug Mendelian randomization study
  96. Population pharmacokinetics of meropenem in critically ill patients
  97. Comparison of the ability of newly inflammatory markers to predict complicated appendicitis
  98. Comparative morphology of the cruciate ligaments: A radiological study
  99. Immune landscape of hepatocellular carcinoma: The central role of TP53-inducible glycolysis and apoptosis regulator
  100. Serum SIRT3 levels in epilepsy patients and its association with clinical outcomes and severity: A prospective observational study
  101. SHP-1 mediates cigarette smoke extract-induced epithelial–mesenchymal transformation and inflammation in 16HBE cells
  102. Acute hyper-hypoxia accelerates the development of depression in mice via the IL-6/PGC1α/MFN2 signaling pathway
  103. The GJB3 correlates with the prognosis, immune cell infiltration, and therapeutic responses in lung adenocarcinoma
  104. Physical fitness and blood parameters outcomes of breast cancer survivor in a low-intensity circuit resistance exercise program
  105. Exploring anesthetic-induced gene expression changes and immune cell dynamics in atrial tissue post-coronary artery bypass graft surgery
  106. Empagliflozin improves aortic injury in obese mice by regulating fatty acid metabolism
  107. Analysis of the risk factors of the radiation-induced encephalopathy in nasopharyngeal carcinoma: A retrospective cohort study
  108. Reproductive outcomes in women with BRCA 1/2 germline mutations: A retrospective observational study and literature review
  109. Evaluation of upper airway ultrasonographic measurements in predicting difficult intubation: A cross-section of the Turkish population
  110. Prognostic and diagnostic value of circulating IGFBP2 in pancreatic cancer
  111. Postural stability after operative reconstruction of the AFTL in chronic ankle instability comparing three different surgical techniques
  112. Research trends related to emergence agitation in the post-anaesthesia care unit from 2001 to 2023: A bibliometric analysis
  113. Frequency and clinicopathological correlation of gastrointestinal polyps: A six-year single center experience
  114. ACSL4 mediates inflammatory bowel disease and contributes to LPS-induced intestinal epithelial cell dysfunction by activating ferroptosis and inflammation
  115. Affibody-based molecular probe 99mTc-(HE)3ZHER2:V2 for non-invasive HER2 detection in ovarian and breast cancer xenografts
  116. Effectiveness of nutritional support for clinical outcomes in gastric cancer patients: A meta-analysis of randomized controlled trials
  117. The relationship between IFN-γ, IL-10, IL-6 cytokines, and severity of the condition with serum zinc and Fe in children infected with Mycoplasma pneumoniae
  118. Paraquat disrupts the blood–brain barrier by increasing IL-6 expression and oxidative stress through the activation of PI3K/AKT signaling pathway
  119. Sleep quality associate with the increased prevalence of cognitive impairment in coronary artery disease patients: A retrospective case–control study
  120. Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway
  121. Association of polymorphisms in FBN1, MYH11, and TGF-β signaling-related genes with susceptibility of sporadic thoracic aortic aneurysm and dissection in the Zhejiang Han population
  122. Application value of multi-parameter magnetic resonance image-transrectal ultrasound cognitive fusion in prostate biopsy
  123. Laboratory variables‐based artificial neural network models for predicting fatty liver disease: A retrospective study
  124. Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p
  125. Sepsis induces the cardiomyocyte apoptosis and cardiac dysfunction through activation of YAP1/Serpine1/caspase-3 pathway
  126. Assessment of iron metabolism and iron deficiency in incident patients on incident continuous ambulatory peritoneal dialysis
  127. Tibial periosteum flap combined with autologous bone grafting in the treatment of Gustilo-IIIB/IIIC open tibial fractures
  128. The application of intravenous general anesthesia under nasopharyngeal airway assisted ventilation undergoing ureteroscopic holmium laser lithotripsy: A prospective, single-center, controlled trial
  129. Long intergenic noncoding RNA for IGF2BP2 stability suppresses gastric cancer cell apoptosis by inhibiting the maturation of microRNA-34a
  130. Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis
  131. Parental control attitudes over their pre-school children’s diet
  132. The role of auto-HSCT in extranodal natural killer/T cell lymphoma
  133. Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy
  134. Echinacoside inhibits PASMCs calcium overload to prevent hypoxic pulmonary artery remodeling by regulating TRPC1/4/6 and calmodulin
  135. ADAR1 plays a protective role in proximal tubular cells under high glucose conditions by attenuating the PI3K/AKT/mTOR signaling pathway
  136. The risk of cancer among insulin glargine users in Lithuania: A retrospective population-based study
  137. The unusual location of primary hydatid cyst: A case series study
  138. Intraoperative changes in electrophysiological monitoring can be used to predict clinical outcomes in patients with spinal cavernous malformation
  139. Obesity and risk of placenta accreta spectrum: A meta-analysis
  140. Shikonin alleviates asthma phenotypes in mice via an airway epithelial STAT3-dependent mechanism
  141. NSUN6 and HTR7 disturbed the stability of carotid atherosclerotic plaques by regulating the immune responses of macrophages
  142. The effect of COVID-19 lockdown on admission rates in Maternity Hospital
  143. Temporal muscle thickness is not a prognostic predictor in patients with high-grade glioma, an experience at two centers in China
  144. Luteolin alleviates cerebral ischemia/reperfusion injury by regulating cell pyroptosis
  145. Therapeutic role of respiratory exercise in patients with tuberculous pleurisy
  146. Effects of CFTR-ENaC on spinal cord edema after spinal cord injury
  147. Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human mesenchymal stem cells
  148. DMD mutations in pediatric patients with phenotypes of Duchenne/Becker muscular dystrophy
  149. Combination of C-reactive protein and fibrinogen-to-albumin ratio as a novel predictor of all-cause mortality in heart failure patients
  150. Significant role and the underly mechanism of cullin-1 in chronic obstructive pulmonary disease
  151. Ferroptosis-related prognostic model of mantle cell lymphoma
  152. Observation of choking reaction and other related indexes in elderly painless fiberoptic bronchoscopy with transnasal high-flow humidification oxygen therapy
  153. A bibliometric analysis of Prader-Willi syndrome from 2002 to 2022
  154. The causal effects of childhood sunburn occasions on melanoma: A univariable and multivariable Mendelian randomization study
  155. Oxidative stress regulates glycogen synthase kinase-3 in lymphocytes of diabetes mellitus patients complicated with cerebral infarction
  156. Role of COX6C and NDUFB3 in septic shock and stroke
  157. Trends in disease burden of type 2 diabetes, stroke, and hypertensive heart disease attributable to high BMI in China: 1990–2019
  158. Purinergic P2X7 receptor mediates hyperoxia-induced injury in pulmonary microvascular endothelial cells via NLRP3-mediated pyroptotic pathway
  159. Investigating the role of oviductal mucosa–endometrial co-culture in modulating factors relevant to embryo implantation
  160. Analgesic effect of external oblique intercostal block in laparoscopic cholecystectomy: A retrospective study
  161. Elevated serum miR-142-5p correlates with ischemic lesions and both NSE and S100β in ischemic stroke patients
  162. Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
  163. Risk factors for progressive kyphosis after percutaneous kyphoplasty in osteoporotic vertebral compression fracture
  164. Predictive role of neuron-specific enolase and S100-β in early neurological deterioration and unfavorable prognosis in patients with ischemic stroke
  165. The potential risk factors of postoperative cognitive dysfunction for endovascular therapy in acute ischemic stroke with general anesthesia
  166. Fluoxetine inhibited RANKL-induced osteoclastic differentiation in vitro
  167. Detection of serum FOXM1 and IGF2 in patients with ARDS and their correlation with disease and prognosis
  168. Rhein promotes skin wound healing by activating the PI3K/AKT signaling pathway
  169. Differences in mortality risk by levels of physical activity among persons with disabilities in South Korea
  170. Review Articles
  171. Cutaneous signs of selected cardiovascular disorders: A narrative review
  172. XRCC1 and hOGG1 polymorphisms and endometrial carcinoma: A meta-analysis
  173. A narrative review on adverse drug reactions of COVID-19 treatments on the kidney
  174. Emerging role and function of SPDL1 in human health and diseases
  175. Adverse reactions of piperacillin: A literature review of case reports
  176. Molecular mechanism and intervention measures of microvascular complications in diabetes
  177. Regulation of mesenchymal stem cell differentiation by autophagy
  178. Molecular landscape of borderline ovarian tumours: A systematic review
  179. Advances in synthetic lethality modalities for glioblastoma multiforme
  180. Investigating hormesis, aging, and neurodegeneration: From bench to clinics
  181. Frankincense: A neuronutrient to approach Parkinson’s disease treatment
  182. Sox9: A potential regulator of cancer stem cells in osteosarcoma
  183. Early detection of cardiovascular risk markers through non-invasive ultrasound methodologies in periodontitis patients
  184. Advanced neuroimaging and criminal interrogation in lie detection
  185. Maternal factors for neural tube defects in offspring: An umbrella review
  186. The chemoprotective hormetic effects of rosmarinic acid
  187. CBD’s potential impact on Parkinson’s disease: An updated overview
  188. Progress in cytokine research for ARDS: A comprehensive review
  189. Utilizing reactive oxygen species-scavenging nanoparticles for targeting oxidative stress in the treatment of ischemic stroke: A review
  190. NRXN1-related disorders, attempt to better define clinical assessment
  191. Lidocaine infusion for the treatment of complex regional pain syndrome: Case series and literature review
  192. Trends and future directions of autophagy in osteosarcoma: A bibliometric analysis
  193. Iron in ventricular remodeling and aneurysms post-myocardial infarction
  194. Case Reports
  195. Sirolimus potentiated angioedema: A case report and review of the literature
  196. Identification of mixed anaerobic infections after inguinal hernia repair based on metagenomic next-generation sequencing: A case report
  197. Successful treatment with bortezomib in combination with dexamethasone in a middle-aged male with idiopathic multicentric Castleman’s disease: A case report
  198. Complete heart block associated with hepatitis A infection in a female child with fatal outcome
  199. Elevation of D-dimer in eosinophilic gastrointestinal diseases in the absence of venous thrombosis: A case series and literature review
  200. Four years of natural progressive course: A rare case report of juvenile Xp11.2 translocations renal cell carcinoma with TFE3 gene fusion
  201. Advancing prenatal diagnosis: Echocardiographic detection of Scimitar syndrome in China – A case series
  202. Outcomes and complications of hemodialysis in patients with renal cancer following bilateral nephrectomy
  203. Anti-HMGCR myopathy mimicking facioscapulohumeral muscular dystrophy
  204. Recurrent opportunistic infections in a HIV-negative patient with combined C6 and NFKB1 mutations: A case report, pedigree analysis, and literature review
  205. Letter to the Editor
  206. Letter to the Editor: Total parenteral nutrition-induced Wernicke’s encephalopathy after oncologic gastrointestinal surgery
  207. Erratum
  208. Erratum to “Bladder-embedded ectopic intrauterine device with calculus”
  209. Retraction
  210. Retraction of “XRCC1 and hOGG1 polymorphisms and endometrial carcinoma: A meta-analysis”
  211. Corrigendum
  212. Corrigendum to “Investigating hormesis, aging, and neurodegeneration: From bench to clinics”
  213. Corrigendum to “Frankincense: A neuronutrient to approach Parkinson’s disease treatment”
  214. Special Issue The evolving saga of RNAs from bench to bedside - Part II
  215. Machine-learning-based prediction of a diagnostic model using autophagy-related genes based on RNA sequencing for patients with papillary thyroid carcinoma
  216. Unlocking the future of hepatocellular carcinoma treatment: A comprehensive analysis of disulfidptosis-related lncRNAs for prognosis and drug screening
  217. Elevated mRNA level indicates FSIP1 promotes EMT and gastric cancer progression by regulating fibroblasts in tumor microenvironment
  218. Special Issue Advancements in oncology: bridging clinical and experimental research - Part I
  219. Ultrasound-guided transperineal vs transrectal prostate biopsy: A meta-analysis of diagnostic accuracy and complication rates
  220. Assessment of diagnostic value of unilateral systematic biopsy combined with targeted biopsy in detecting clinically significant prostate cancer
  221. SENP7 inhibits glioblastoma metastasis and invasion by dissociating SUMO2/3 binding to specific target proteins
  222. MARK1 suppress malignant progression of hepatocellular carcinoma and improves sorafenib resistance through negatively regulating POTEE
  223. Analysis of postoperative complications in bladder cancer patients
  224. Carboplatin combined with arsenic trioxide versus carboplatin combined with docetaxel treatment for LACC: A randomized, open-label, phase II clinical study
  225. Special Issue Exploring the biological mechanism of human diseases based on MultiOmics Technology - Part I
  226. Comprehensive pan-cancer investigation of carnosine dipeptidase 1 and its prospective prognostic significance in hepatocellular carcinoma
  227. Identification of signatures associated with microsatellite instability and immune characteristics to predict the prognostic risk of colon cancer
  228. Single-cell analysis identified key macrophage subpopulations associated with atherosclerosis
https://doi.org/10.1515/med-2024-1073
Keywords for this article
irisin; chondrogenic differentiation; lncRNAs; co-expression; ceRNAs
Creative Commons
BY 4.0

Articles in the same Issue

  1. Research Articles
  2. EDNRB inhibits the growth and migration of prostate cancer cells by activating the cGMP-PKG pathway
  3. STK11 (LKB1) mutation suppresses ferroptosis in lung adenocarcinoma by facilitating monounsaturated fatty acid synthesis
  4. Association of SOX6 gene polymorphisms with Kashin-Beck disease risk in the Chinese Han population
  5. The pyroptosis-related signature predicts prognosis and influences the tumor immune microenvironment in dedifferentiated liposarcoma
  6. METTL3 attenuates ferroptosis sensitivity in lung cancer via modulating TFRC
  7. Identification and validation of molecular subtypes and prognostic signature for stage I and stage II gastric cancer based on neutrophil extracellular traps
  8. Novel lumbar plexus block versus femoral nerve block for analgesia and motor recovery after total knee arthroplasty
  9. Correlation between ABCB1 and OLIG2 polymorphisms and the severity and prognosis of patients with cerebral infarction
  10. Study on the radiotherapy effect and serum neutral granulocyte lymphocyte ratio and inflammatory factor expression of nasopharyngeal carcinoma
  11. Transcriptome analysis of effects of Tecrl deficiency on cardiometabolic and calcium regulation in cardiac tissue
  12. Aflatoxin B1 induces infertility, fetal deformities, and potential therapies
  13. Serum levels of HMW adiponectin and its receptors are associated with cytokine levels and clinical characteristics in chronic obstructive pulmonary disease
  14. METTL3-mediated methylation of CYP2C19 mRNA may aggravate clopidogrel resistance in ischemic stroke patients
  15. Understand how machine learning impact lung cancer research from 2010 to 2021: A bibliometric analysis
  16. Pressure ulcers in German hospitals: Analysis of reimbursement and length of stay
  17. Metformin plus L-carnitine enhances brown/beige adipose tissue activity via Nrf2/HO-1 signaling to reduce lipid accumulation and inflammation in murine obesity
  18. Downregulation of carbonic anhydrase IX expression in mouse xenograft nasopharyngeal carcinoma model via doxorubicin nanobubble combined with ultrasound
  19. Feasibility of 3-dimensional printed models in simulated training and teaching of transcatheter aortic valve replacement
  20. miR-335-3p improves type II diabetes mellitus by IGF-1 regulating macrophage polarization
  21. The analyses of human MCPH1 DNA repair machinery and genetic variations
  22. Activation of Piezo1 increases the sensitivity of breast cancer to hyperthermia therapy
  23. Comprehensive analysis based on the disulfidptosis-related genes identifies hub genes and immune infiltration for pancreatic adenocarcinoma
  24. Changes of serum CA125 and PGE2 before and after high-intensity focused ultrasound combined with GnRH-a in treatment of patients with adenomyosis
  25. The clinical value of the hepatic venous pressure gradient in patients undergoing hepatic resection for hepatocellular carcinoma with or without liver cirrhosis
  26. Development and validation of a novel model to predict pulmonary embolism in cardiology suspected patients: A 10-year retrospective analysis
  27. Downregulation of lncRNA XLOC_032768 in diabetic patients predicts the occurrence of diabetic nephropathy
  28. Circ_0051428 targeting miR-885-3p/MMP2 axis enhances the malignancy of cervical cancer
  29. Effectiveness of ginkgo diterpene lactone meglumine on cognitive function in patients with acute ischemic stroke
  30. The construction of a novel prognostic prediction model for glioma based on GWAS-identified prognostic-related risk loci
  31. Evaluating the impact of childhood BMI on the risk of coronavirus disease 2019: A Mendelian randomization study
  32. Lactate dehydrogenase to albumin ratio is associated with in-hospital mortality in patients with acute heart failure: Data from the MIMIC-III database
  33. CD36-mediated podocyte lipotoxicity promotes foot process effacement
  34. Efficacy of etonogestrel subcutaneous implants versus the levonorgestrel-releasing intrauterine system in the conservative treatment of adenomyosis
  35. FLRT2 mediates chondrogenesis of nasal septal cartilage and mandibular condyle cartilage
  36. Challenges in treating primary immune thrombocytopenia patients undergoing COVID-19 vaccination: A retrospective study
  37. Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway
  38. Phospholipid transfer protein ameliorates sepsis-induced cardiac dysfunction through NLRP3 inflammasome inhibition
  39. Postoperative cognitive dysfunction in elderly patients with colorectal cancer: A randomized controlled study comparing goal-directed and conventional fluid therapy
  40. Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction
  41. High SEC61A1 expression predicts poor outcome of acute myeloid leukemia
  42. Comparison of polymerase chain reaction and next-generation sequencing with conventional urine culture for the diagnosis of urinary tract infections: A meta-analysis
  43. Secreted frizzled-related protein 5 protects against renal fibrosis by inhibiting Wnt/β-catenin pathway
  44. Pan-cancer and single-cell analysis of actin cytoskeleton genes related to disulfidptosis
  45. Overexpression of miR-532-5p restrains oxidative stress response of chondrocytes in nontraumatic osteonecrosis of the femoral head by inhibiting ABL1
  46. Autologous liver transplantation for unresectable hepatobiliary malignancies in enhanced recovery after surgery model
  47. Clinical analysis of incomplete rupture of the uterus secondary to previous cesarean section
  48. Abnormal sleep duration is associated with sarcopenia in older Chinese people: A large retrospective cross-sectional study
  49. No genetic causality between obesity and benign paroxysmal vertigo: A two-sample Mendelian randomization study
  50. Identification and validation of autophagy-related genes in SSc
  51. Long non-coding RNA SRA1 suppresses radiotherapy resistance in esophageal squamous cell carcinoma by modulating glycolytic reprogramming
  52. Evaluation of quality of life in patients with schizophrenia: An inpatient social welfare institution-based cross-sectional study
  53. The possible role of oxidative stress marker glutathione in the assessment of cognitive impairment in multiple sclerosis
  54. Compilation of a self-management assessment scale for postoperative patients with aortic dissection
  55. Left atrial appendage closure in conjunction with radiofrequency ablation: Effects on left atrial functioning in patients with paroxysmal atrial fibrillation
  56. Effect of anterior femoral cortical notch grade on postoperative function and complications during TKA surgery: A multicenter, retrospective study
  57. Clinical characteristics and assessment of risk factors in patients with influenza A-induced severe pneumonia after the prevalence of SARS-CoV-2
  58. Analgesia nociception index is an indicator of laparoscopic trocar insertion-induced transient nociceptive stimuli
  59. High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression
  60. Factors influencing cardiovascular system-related post-COVID-19 sequelae: A single-center cohort study
  61. HOXD10 regulates intestinal permeability and inhibits inflammation of dextran sulfate sodium-induced ulcerative colitis through the inactivation of the Rho/ROCK/MMPs axis
  62. Mesenchymal stem cell-derived exosomal miR-26a induces ferroptosis, suppresses hepatic stellate cell activation, and ameliorates liver fibrosis by modulating SLC7A11
  63. Endovascular thrombectomy versus intravenous thrombolysis for primary distal, medium vessel occlusion in acute ischemic stroke
  64. ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis
  65. Prognostic value of EIF5A2 in solid tumors: A meta-analysis and bioinformatics analysis
  66. The role of enhanced expression of Cx43 in patients with ulcerative colitis
  67. Choosing a COVID-19 vaccination site might be driven by anxiety and body vigilance
  68. Role of ICAM-1 in triple-negative breast cancer
  69. Cost-effectiveness of ambroxol in the treatment of Gaucher disease type 2
  70. HLA-DRB5 promotes immune thrombocytopenia via activating CD8+ T cells
  71. Efficacy and factors of myofascial release therapy combined with electrical and magnetic stimulation in the treatment of chronic pelvic pain syndrome
  72. Efficacy of tacrolimus monotherapy in primary membranous nephropathy
  73. Mechanisms of Tripterygium wilfordii Hook F on treating rheumatoid arthritis explored by network pharmacology analysis and molecular docking
  74. FBXO45 levels regulated ferroptosis renal tubular epithelial cells in a model of diabetic nephropathy by PLK1
  75. Optimizing anesthesia strategies to NSCLC patients in VATS procedures: Insights from drug requirements and patient recovery patterns
  76. Alpha-lipoic acid upregulates the PPARγ/NRF2/GPX4 signal pathway to inhibit ferroptosis in the pathogenesis of unexplained recurrent pregnancy loss
  77. Correlation between fat-soluble vitamin levels and inflammatory factors in paediatric community-acquired pneumonia: A prospective study
  78. CD1d affects the proliferation, migration, and apoptosis of human papillary thyroid carcinoma TPC-1 cells via regulating MAPK/NF-κB signaling pathway
  79. miR-let-7a inhibits sympathetic nerve remodeling after myocardial infarction by downregulating the expression of nerve growth factor
  80. Immune response analysis of solid organ transplantation recipients inoculated with inactivated COVID-19 vaccine: A retrospective analysis
  81. The H2Valdien derivatives regulate the epithelial–mesenchymal transition of hepatoma carcinoma cells through the Hedgehog signaling pathway
  82. Clinical efficacy of dexamethasone combined with isoniazid in the treatment of tuberculous meningitis and its effect on peripheral blood T cell subsets
  83. Comparison of short-segment and long-segment fixation in treatment of degenerative scoliosis and analysis of factors associated with adjacent spondylolisthesis
  84. Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathway
  85. Methylation regulation for FUNDC1 stability in childhood leukemia was up-regulated and facilitates metastasis and reduces ferroptosis of leukemia through mitochondrial damage by FBXL2
  86. Correlation of single-fiber electromyography studies and functional status in patients with amyotrophic lateral sclerosis
  87. Risk factors of postoperative airway obstruction complications in children with oral floor mass
  88. Expression levels and clinical significance of serum miR-19a/CCL20 in patients with acute cerebral infarction
  89. Physical activity and mental health trends in Korean adolescents: Analyzing the impact of the COVID-19 pandemic from 2018 to 2022
  90. Evaluating anemia in HIV-infected patients using chest CT
  91. Ponticulus posticus and skeletal malocclusion: A pilot study in a Southern Italian pre-orthodontic court
  92. Causal association of circulating immune cells and lymphoma: A Mendelian randomization study
  93. Assessment of the renal function and fibrosis indexes of conventional western medicine with Chinese medicine for dredging collaterals on treating renal fibrosis: A systematic review and meta-analysis
  94. Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer
  95. New target-HMGCR inhibitors for the treatment of primary sclerosing cholangitis: A drug Mendelian randomization study
  96. Population pharmacokinetics of meropenem in critically ill patients
  97. Comparison of the ability of newly inflammatory markers to predict complicated appendicitis
  98. Comparative morphology of the cruciate ligaments: A radiological study
  99. Immune landscape of hepatocellular carcinoma: The central role of TP53-inducible glycolysis and apoptosis regulator
  100. Serum SIRT3 levels in epilepsy patients and its association with clinical outcomes and severity: A prospective observational study
  101. SHP-1 mediates cigarette smoke extract-induced epithelial–mesenchymal transformation and inflammation in 16HBE cells
  102. Acute hyper-hypoxia accelerates the development of depression in mice via the IL-6/PGC1α/MFN2 signaling pathway
  103. The GJB3 correlates with the prognosis, immune cell infiltration, and therapeutic responses in lung adenocarcinoma
  104. Physical fitness and blood parameters outcomes of breast cancer survivor in a low-intensity circuit resistance exercise program
  105. Exploring anesthetic-induced gene expression changes and immune cell dynamics in atrial tissue post-coronary artery bypass graft surgery
  106. Empagliflozin improves aortic injury in obese mice by regulating fatty acid metabolism
  107. Analysis of the risk factors of the radiation-induced encephalopathy in nasopharyngeal carcinoma: A retrospective cohort study
  108. Reproductive outcomes in women with BRCA 1/2 germline mutations: A retrospective observational study and literature review
  109. Evaluation of upper airway ultrasonographic measurements in predicting difficult intubation: A cross-section of the Turkish population
  110. Prognostic and diagnostic value of circulating IGFBP2 in pancreatic cancer
  111. Postural stability after operative reconstruction of the AFTL in chronic ankle instability comparing three different surgical techniques
  112. Research trends related to emergence agitation in the post-anaesthesia care unit from 2001 to 2023: A bibliometric analysis
  113. Frequency and clinicopathological correlation of gastrointestinal polyps: A six-year single center experience
  114. ACSL4 mediates inflammatory bowel disease and contributes to LPS-induced intestinal epithelial cell dysfunction by activating ferroptosis and inflammation
  115. Affibody-based molecular probe 99mTc-(HE)3ZHER2:V2 for non-invasive HER2 detection in ovarian and breast cancer xenografts
  116. Effectiveness of nutritional support for clinical outcomes in gastric cancer patients: A meta-analysis of randomized controlled trials
  117. The relationship between IFN-γ, IL-10, IL-6 cytokines, and severity of the condition with serum zinc and Fe in children infected with Mycoplasma pneumoniae
  118. Paraquat disrupts the blood–brain barrier by increasing IL-6 expression and oxidative stress through the activation of PI3K/AKT signaling pathway
  119. Sleep quality associate with the increased prevalence of cognitive impairment in coronary artery disease patients: A retrospective case–control study
  120. Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway
  121. Association of polymorphisms in FBN1, MYH11, and TGF-β signaling-related genes with susceptibility of sporadic thoracic aortic aneurysm and dissection in the Zhejiang Han population
  122. Application value of multi-parameter magnetic resonance image-transrectal ultrasound cognitive fusion in prostate biopsy
  123. Laboratory variables‐based artificial neural network models for predicting fatty liver disease: A retrospective study
  124. Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p
  125. Sepsis induces the cardiomyocyte apoptosis and cardiac dysfunction through activation of YAP1/Serpine1/caspase-3 pathway
  126. Assessment of iron metabolism and iron deficiency in incident patients on incident continuous ambulatory peritoneal dialysis
  127. Tibial periosteum flap combined with autologous bone grafting in the treatment of Gustilo-IIIB/IIIC open tibial fractures
  128. The application of intravenous general anesthesia under nasopharyngeal airway assisted ventilation undergoing ureteroscopic holmium laser lithotripsy: A prospective, single-center, controlled trial
  129. Long intergenic noncoding RNA for IGF2BP2 stability suppresses gastric cancer cell apoptosis by inhibiting the maturation of microRNA-34a
  130. Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis
  131. Parental control attitudes over their pre-school children’s diet
  132. The role of auto-HSCT in extranodal natural killer/T cell lymphoma
  133. Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy
  134. Echinacoside inhibits PASMCs calcium overload to prevent hypoxic pulmonary artery remodeling by regulating TRPC1/4/6 and calmodulin
  135. ADAR1 plays a protective role in proximal tubular cells under high glucose conditions by attenuating the PI3K/AKT/mTOR signaling pathway
  136. The risk of cancer among insulin glargine users in Lithuania: A retrospective population-based study
  137. The unusual location of primary hydatid cyst: A case series study
  138. Intraoperative changes in electrophysiological monitoring can be used to predict clinical outcomes in patients with spinal cavernous malformation
  139. Obesity and risk of placenta accreta spectrum: A meta-analysis
  140. Shikonin alleviates asthma phenotypes in mice via an airway epithelial STAT3-dependent mechanism
  141. NSUN6 and HTR7 disturbed the stability of carotid atherosclerotic plaques by regulating the immune responses of macrophages
  142. The effect of COVID-19 lockdown on admission rates in Maternity Hospital
  143. Temporal muscle thickness is not a prognostic predictor in patients with high-grade glioma, an experience at two centers in China
  144. Luteolin alleviates cerebral ischemia/reperfusion injury by regulating cell pyroptosis
  145. Therapeutic role of respiratory exercise in patients with tuberculous pleurisy
  146. Effects of CFTR-ENaC on spinal cord edema after spinal cord injury
  147. Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human mesenchymal stem cells
  148. DMD mutations in pediatric patients with phenotypes of Duchenne/Becker muscular dystrophy
  149. Combination of C-reactive protein and fibrinogen-to-albumin ratio as a novel predictor of all-cause mortality in heart failure patients
  150. Significant role and the underly mechanism of cullin-1 in chronic obstructive pulmonary disease
  151. Ferroptosis-related prognostic model of mantle cell lymphoma
  152. Observation of choking reaction and other related indexes in elderly painless fiberoptic bronchoscopy with transnasal high-flow humidification oxygen therapy
  153. A bibliometric analysis of Prader-Willi syndrome from 2002 to 2022
  154. The causal effects of childhood sunburn occasions on melanoma: A univariable and multivariable Mendelian randomization study
  155. Oxidative stress regulates glycogen synthase kinase-3 in lymphocytes of diabetes mellitus patients complicated with cerebral infarction
  156. Role of COX6C and NDUFB3 in septic shock and stroke
  157. Trends in disease burden of type 2 diabetes, stroke, and hypertensive heart disease attributable to high BMI in China: 1990–2019
  158. Purinergic P2X7 receptor mediates hyperoxia-induced injury in pulmonary microvascular endothelial cells via NLRP3-mediated pyroptotic pathway
  159. Investigating the role of oviductal mucosa–endometrial co-culture in modulating factors relevant to embryo implantation
  160. Analgesic effect of external oblique intercostal block in laparoscopic cholecystectomy: A retrospective study
  161. Elevated serum miR-142-5p correlates with ischemic lesions and both NSE and S100β in ischemic stroke patients
  162. Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
  163. Risk factors for progressive kyphosis after percutaneous kyphoplasty in osteoporotic vertebral compression fracture
  164. Predictive role of neuron-specific enolase and S100-β in early neurological deterioration and unfavorable prognosis in patients with ischemic stroke
  165. The potential risk factors of postoperative cognitive dysfunction for endovascular therapy in acute ischemic stroke with general anesthesia
  166. Fluoxetine inhibited RANKL-induced osteoclastic differentiation in vitro
  167. Detection of serum FOXM1 and IGF2 in patients with ARDS and their correlation with disease and prognosis
  168. Rhein promotes skin wound healing by activating the PI3K/AKT signaling pathway
  169. Differences in mortality risk by levels of physical activity among persons with disabilities in South Korea
  170. Review Articles
  171. Cutaneous signs of selected cardiovascular disorders: A narrative review
  172. XRCC1 and hOGG1 polymorphisms and endometrial carcinoma: A meta-analysis
  173. A narrative review on adverse drug reactions of COVID-19 treatments on the kidney
  174. Emerging role and function of SPDL1 in human health and diseases
  175. Adverse reactions of piperacillin: A literature review of case reports
  176. Molecular mechanism and intervention measures of microvascular complications in diabetes
  177. Regulation of mesenchymal stem cell differentiation by autophagy
  178. Molecular landscape of borderline ovarian tumours: A systematic review
  179. Advances in synthetic lethality modalities for glioblastoma multiforme
  180. Investigating hormesis, aging, and neurodegeneration: From bench to clinics
  181. Frankincense: A neuronutrient to approach Parkinson’s disease treatment
  182. Sox9: A potential regulator of cancer stem cells in osteosarcoma
  183. Early detection of cardiovascular risk markers through non-invasive ultrasound methodologies in periodontitis patients
  184. Advanced neuroimaging and criminal interrogation in lie detection
  185. Maternal factors for neural tube defects in offspring: An umbrella review
  186. The chemoprotective hormetic effects of rosmarinic acid
  187. CBD’s potential impact on Parkinson’s disease: An updated overview
  188. Progress in cytokine research for ARDS: A comprehensive review
  189. Utilizing reactive oxygen species-scavenging nanoparticles for targeting oxidative stress in the treatment of ischemic stroke: A review
  190. NRXN1-related disorders, attempt to better define clinical assessment
  191. Lidocaine infusion for the treatment of complex regional pain syndrome: Case series and literature review
  192. Trends and future directions of autophagy in osteosarcoma: A bibliometric analysis
  193. Iron in ventricular remodeling and aneurysms post-myocardial infarction
  194. Case Reports
  195. Sirolimus potentiated angioedema: A case report and review of the literature
  196. Identification of mixed anaerobic infections after inguinal hernia repair based on metagenomic next-generation sequencing: A case report
  197. Successful treatment with bortezomib in combination with dexamethasone in a middle-aged male with idiopathic multicentric Castleman’s disease: A case report
  198. Complete heart block associated with hepatitis A infection in a female child with fatal outcome
  199. Elevation of D-dimer in eosinophilic gastrointestinal diseases in the absence of venous thrombosis: A case series and literature review
  200. Four years of natural progressive course: A rare case report of juvenile Xp11.2 translocations renal cell carcinoma with TFE3 gene fusion
  201. Advancing prenatal diagnosis: Echocardiographic detection of Scimitar syndrome in China – A case series
  202. Outcomes and complications of hemodialysis in patients with renal cancer following bilateral nephrectomy
  203. Anti-HMGCR myopathy mimicking facioscapulohumeral muscular dystrophy
  204. Recurrent opportunistic infections in a HIV-negative patient with combined C6 and NFKB1 mutations: A case report, pedigree analysis, and literature review
  205. Letter to the Editor
  206. Letter to the Editor: Total parenteral nutrition-induced Wernicke’s encephalopathy after oncologic gastrointestinal surgery
  207. Erratum
  208. Erratum to “Bladder-embedded ectopic intrauterine device with calculus”
  209. Retraction
  210. Retraction of “XRCC1 and hOGG1 polymorphisms and endometrial carcinoma: A meta-analysis”
  211. Corrigendum
  212. Corrigendum to “Investigating hormesis, aging, and neurodegeneration: From bench to clinics”
  213. Corrigendum to “Frankincense: A neuronutrient to approach Parkinson’s disease treatment”
  214. Special Issue The evolving saga of RNAs from bench to bedside - Part II
  215. Machine-learning-based prediction of a diagnostic model using autophagy-related genes based on RNA sequencing for patients with papillary thyroid carcinoma
  216. Unlocking the future of hepatocellular carcinoma treatment: A comprehensive analysis of disulfidptosis-related lncRNAs for prognosis and drug screening
  217. Elevated mRNA level indicates FSIP1 promotes EMT and gastric cancer progression by regulating fibroblasts in tumor microenvironment
  218. Special Issue Advancements in oncology: bridging clinical and experimental research - Part I
  219. Ultrasound-guided transperineal vs transrectal prostate biopsy: A meta-analysis of diagnostic accuracy and complication rates
  220. Assessment of diagnostic value of unilateral systematic biopsy combined with targeted biopsy in detecting clinically significant prostate cancer
  221. SENP7 inhibits glioblastoma metastasis and invasion by dissociating SUMO2/3 binding to specific target proteins
  222. MARK1 suppress malignant progression of hepatocellular carcinoma and improves sorafenib resistance through negatively regulating POTEE
  223. Analysis of postoperative complications in bladder cancer patients
  224. Carboplatin combined with arsenic trioxide versus carboplatin combined with docetaxel treatment for LACC: A randomized, open-label, phase II clinical study
  225. Special Issue Exploring the biological mechanism of human diseases based on MultiOmics Technology - Part I
  226. Comprehensive pan-cancer investigation of carnosine dipeptidase 1 and its prospective prognostic significance in hepatocellular carcinoma
  227. Identification of signatures associated with microsatellite instability and immune characteristics to predict the prognostic risk of colon cancer
  228. Single-cell analysis identified key macrophage subpopulations associated with atherosclerosis
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 25.12.2025 from https://www.degruyterbrill.com/document/doi/10.1515/med-2024-1073/html
Scroll to top button