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Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4

  • Luming Jin , Chaoyang Chen , Lipeng Huang , Qingyu Sun and Liang Bu EMAIL logo
Published/Copyright: December 16, 2021
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Open Medicine
From the journal Open Medicine Volume 17 Issue 1

Abstract

Long noncoding RNA (lncRNA), specifically the upregulation of lncRNA NR2F1 antisense RNA 1 (NR2F1-AS1), has been involved in the progression of non-small cell lung cancer (NSCLC), but the mechanisms that underlie this remain unclear. In this study, the expression of NR2F1-AS1, miR-363-3p, and SOX4 was assessed in NSCLC cells. A loss-of-function assay was used to measure the tumorigenicity of NSCLC cells. The glycolysis and glutamine metabolism of NSCLC cells was also measured via extracellular acidification rate, consumption of glucose and glutamine, and production of lactate and ATP. The relationships among NR2F1-AS1, miR-363-3p, and SOX4 were detected via dual-luciferase reporter assay. HK-2, GLS1, and SOX4 levels were also analyzed. We found that both NSCLC tissues and cells had higher levels of NR2F1-AS1. Silencing of NR2F1-AS1 inhibited the tumorigenicity of cells in vitro and reduced the glycolysis and glutamine metabolism of NSCLC cells. Regarding its mechanism, NR2F1-AS1 positively regulated the SOX4 level by sponging miR-363-3p. Furthermore, miR-363-3p inhibition or SOX4 overexpression reversed the repressing role of sh-NR2F1-AS1 in the tumorigenicity of NSCLC cells. In summary, NR2F1-AS1 promotes the tumorigenicity of NSCLC cells by regulating miR-363-3p/SOX4.

Keywords: non-small cell lung cancer; glycolysis; NR2F1-AS1; miR-363-3p; SOX4

1 Introduction

Lung cancer, one of the most serious cancers globally, is broadly classified into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). Clinically, most lung cancer patients are diagnosed as NSCLC [1,2]. Despite the current chemotherapeutic and radiotherapeutic treatments, many NSCLC patients still have a poor prognosis due to the lack of a reliable treatment strategy [3]. More recently, the development of targeted therapy and immunotherapy has prolonged the survival of NSCLC patients [4], but treatment outcomes remain unsatisfactory. Therefore, it is extremely vital to find novel therapeutic strategies for NSCLC patients. Emerging evidence has demonstrated that the activation of NSCLC cells is related to glucose consumption and metabolism [5,6]. Inhibiting glucose metabolism can also delay NSCLC progression, which can potentially be a new reliable strategy for NSCLC therapy [7]. Although more knowledge has emerged regarding the metabolic reprogramming of NSCLC, the mechanisms underlying NSCLC glycolysis remain obscure.

Long noncoding RNAs (lncRNAs) include many transcripts that lack protein-coding ability [8,9]. Until now, lncRNAs have now been closely implicated in several biological roles, such as transcriptional regulation [10], RNA processing [11], epigenetic modification [12], and posttranslational modification [13]. Aberrant lncRNA expression has vital functions during tumorigenesis and glycolysis [14,15]. In fact, various dysregulations of lncRNA have been found regarding the growth, metastasis, and development of NSCLC [16,17]. For example, the elevated expression of hox transcript antisense intergenic RNA in NSCLC tissues is associated with a shorter overall survival time [18,19]. By controlling TDG-mediated acetylation, LINC00467 increased cell growth and metastasis through the Akt pathway in NSCLC cells [20]. He et al. found that lncRNA NNT-AS1 acts as a sponge in regulating YAP1 levels through competition for miR-22-3p, thereby promoting the progression of NSCLC [21]. LINC00243 elevates NSCLC cell proliferation and glycolysis via overexpression of PDK4 by sponging miR-507 [22]. Therefore, tumor-specific lncRNAs are the potential targets for the development of novel strategies against NSCLC [23,24].

A few reports have linked lncRNA NR2F1 antisense RNA 1 (NR2F1-AS1) to tumorigenesis and tumor progression [25,26,27]. However, the extent of the pro-oncogenic effect of NR2F1-AS1 on NSCLC is limited [28]. In this study, we found that NR2F1-AS1 induces tumorigenicity and glycolysis in NSCLC cells. We also demonstrated that NR2F1-AS1 knockdown or miR-363-3p mimics can repress glycolysis in NSCLC cells. Moreover, we observed that NR2F1-AS1 exerts this pro-oncogenic role via miR-363-3p/SOX4. Our data may provide new evidence for targeting NR2F1-AS1 as a promising therapeutic approach for NSCLC.

2 Materials and methods

2.1 Clinical specimen collection

A total of 20 paired NSCLC tumors and normal samples were obtained from Xiang’an Hospital. All subjects gave written informed consent. This study was approved by the Ethics Committee of the Xiang’an Hospital. All samples were stored in liquid nitrogen for use.

2.2 Cell lines and transfection

Human normal bronchial epithelial cell line 16HBE, A549, and H522 cells were purchased from Shanghai Cellular Research Institute and cultured in RPMI-1640 medium (ScienCell, Beijing, China) with 10% fetal bovine serum (FBS) in a 37°C atmosphere with 5% CO2.

The shRNA of NR2F1-AS1 (sh-NR2F1-AS1#1-3), miR-363-3p mimic and inhibitor, pcDNA SOX4 overexpression plasmid, and the NC controls were obtained from Genepharma (Shanghai, China). The cells were transfected with lipofectamine 3000 reagent for 48 h.

2.3 Quantitative real-time PCR (qRT-PCR)

The trizol reagent was utilized, and cDNA was synthesized using the cDNA synthesis kit (Roche, Basel, Switzerland). One Step SYBR PrimeScript RT-PCR kit was used for qRT-PCR. The primers were listed as follows: NR2F1-AS1 (sense: 5′-CATGCCGTGATGTAAGCTGC-3′; antisense: 5′-GCGACTGTTTCACCTCTCCA-3′), miR-363-3p (sense: 5′-GCCGAGAATTGCACGGTATC-3′; antisense: 5′-CTCAACTGGTGTCGTGGA-3′), U6 (sense: 5′-CTCGCTTCGGCAGCACA-3′; antisense: 5′-AACGCTTCACGAATTTGCGT-3′), and β-actin (sense: 5′-CTCGCCTTTGCCGATCC-3′; antisense: 5′-TCTCCATGTCGTCCCAGTTG-3′).

2.4 Cell counting kit 8 (CCK8) and clonogenic assay

NSCLC cells were planted into a 96-well culture. At the indicated time, the cells were treated with CCK8 (Beyotime, Shanghai, China). The OD value was determined at 450 nm using a microplate reader.

For clonogenic assay, NSCLC cells were planted in 2.5 cm dishes for 2 weeks at 37°C. The colonies were dyed with crystal violet (Beyotime, Shanghai, China) and counted via a microscope.

2.5 Transwell assay

The upper transwell unit precoated with matrigel (Corning, China) was used in the invasion assay and not in the migration assay. Cells were planted in the top unit coated with Dulbecco’s modified Eagle’s medium. The RPMI-1640 medium and 15% FBS were supplied into the lower unit. After 12 h, the cells in the lower surface were stained with DAPI and counted.

2.6 Measurement of glycolysis and extracellular acidification rate (ECAR)

Glucose consumption and lactate accumulation were measured using the Glucose Assay Kit and Lactate Assay Kit (Abcam, CA, USA) according to the manufacturer’s instructions.

Cells were consecutively treated with glucose, oligomycin, and 2-deoxyglucose. The ECAR of cells was detected using the Glycolysis Rate Determination Kit and evaluated using the Seahorse XFe Extracellular Flux Analyzer (Seahorse Bioscience Inc., Billerica, MA, USA).

2.7 Western blot analysis

Proteins were separated from the samples using the radioimmunoprecipitation assay buffer lysis buffer. The samples were resolved using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred to nitrocellulose membranes. The blots were incubated with the primary antibodies HK-2, 1, GLS1, and SOX4, followed by the secondary antibody. The blots were identified using enhanced chemiluminescence system.

2.8 Dual-luciferase reporter assay

The sequences of NR2F1-AS1 and SOX4 3'UTR were cloned into wild-type (WT) or mutate-type vectors and cotransfected with either miR-363-3p mimic or miR-NC into cells. The Dual-Luciferase Reporter Assay System was used to calculate the luciferase activity.

2.9 Statistical analysis

Measurements are presented as the mean ± standard deviation. The t-test was conducted for two groups, whereas one-way analysis of variance followed by a post hoc test were conducted for three or more groups using the SPSS 19.0 software (IBM, Armonk, NY, USA). Statistical significance was set at P < 0.05.

3 Results

3.1 Expression of NR2F1-AS1 was upregulated in NSCLC tissues and cell lines

To observe the expression of NR2F1-AS1 in NSCLC tissues and cell lines, the qRT-PCR assay was performed. Expression of NR2F1-AS1 was notably higher in NSCLC samples than that in normal tissues (Figure 1a). Particularly, NR2F1-AS1 levels were enhanced in A549 and H522 cells than that in 16HBE cells (Figure 1b). So, NR2F1-AS1 expression was upregulated in NSCLC tissues and cell lines.

Figure 1 The expression of NR2F1-AS1 in NSCLC tissues and cell lines: (a) the expression of NR2F1-AS1 in the paired NSCLC tumor samples and adjacent samples were measured via qRT-PCR and (b) the NR2F1-AS1 expression in 16HBE cells and NSCLC cell lines (A549 and H522) were measured via qRT-PCR. **P < 0.01.
Figure 1

The expression of NR2F1-AS1 in NSCLC tissues and cell lines: (a) the expression of NR2F1-AS1 in the paired NSCLC tumor samples and adjacent samples were measured via qRT-PCR and (b) the NR2F1-AS1 expression in 16HBE cells and NSCLC cell lines (A549 and H522) were measured via qRT-PCR. **P < 0.01.

3.2 NR2F1-AS1 had an oncogenic effect on the progression of A549 and H522 cells

To determine the biological role of NR2F1-AS1 in NSCLC cells, three shRNAs of NR2F1-AS1 were subjected to a loss-of-function test. Then, sh-NR2F1-AS1#2 were used. After treatment with sh-NR2F1-AS1, NR2F1-AS1 expression was effectively hindered in both A549 and H522 cells (Figure 2a and b). Both CCK8 assay and colony formation data showed that the downregulation of NR2F1-AS1 could reduce growth in NSCLC cells (Figure 2c and d). Moreover, transwell and invasion assays observed that the silencing of NR2F1-AS1 had an antimigrative effect on NSCLC cells (Figure 2e–h). Altogether, these findings confirm that NR2F1-AS1 has an oncogenic effect on the progression of NSCLC cells.

Figure 2 NR2F1-AS1 silencing blocked NSCLC progression in vitro. A549 and H522 cells were transfected with sh-NC or sh-NR2F1-AS1. (a and b) The level of NR2F1-AS1 was assessed to check the transfection efficiency of shRNA1-3. (c) The CCK8 assay, (d) colony formation assay, (e and f) transwell assay, and (g and h) invasion assay were operated to determine NSCLC progression in vitro. **P < 0.01.
Figure 2

NR2F1-AS1 silencing blocked NSCLC progression in vitro. A549 and H522 cells were transfected with sh-NC or sh-NR2F1-AS1. (a and b) The level of NR2F1-AS1 was assessed to check the transfection efficiency of shRNA1-3. (c) The CCK8 assay, (d) colony formation assay, (e and f) transwell assay, and (g and h) invasion assay were operated to determine NSCLC progression in vitro. **P < 0.01.

3.3 Downregulation of NR2F1-AS1 prevented glycolysis in NSCLC cells

Inhibiting glycolysis is a new therapeutic strategy for NSCLC. As shown in Figure 3a and b, NR2F1-AS1 knockdown notably diminished the glucose uptake and lactate production of A549 and H522 cells, demonstrating that NR2F1-AS1 is positively correlated with the glycolysis of NSCLC cells. Similarly, reduction of NR2F1-AS1 inhibited glycolysis in NSCLC cells as determined using ECAR (Figure 3c and d). Moreover, the glutamine consumption and ATP levels of A549 and H522 cells were blocked after the reduction of NR2F1-AS1 (Figure 3e and f), implying that NR2F1-AS1 is positively correlated with the glutamine metabolism of NSCLC cells. Furthermore, the expression of glycolysis rate-limiting enzyme HK-2 and glutamine hydrolase GLS1 were clearly diminished by sh-NR2F1-AS1 (Figure 3g and h). Thus, NR2F1-AS1 inhibition prevented glycolysis in NSCLC cells.

Figure 3 NR2F1-AS1 silencing limited the NSCLC cells’ glycolysis and glutamine metabolism. A549 and H522 cells were transfected with sh-NC or sh-NR2F1-AS1. (a–d) The glucose consumption, lactate production, and ECAR were quantified to assess cell glycolysis. (e and f) Glutamine consumption and ATP release were determined by the corresponding assay. (g and h) Western blot analysis was presented to assess HK-2 and GLS1 expression. **P < 0.01.
Figure 3

NR2F1-AS1 silencing limited the NSCLC cells’ glycolysis and glutamine metabolism. A549 and H522 cells were transfected with sh-NC or sh-NR2F1-AS1. (a–d) The glucose consumption, lactate production, and ECAR were quantified to assess cell glycolysis. (e and f) Glutamine consumption and ATP release were determined by the corresponding assay. (g and h) Western blot analysis was presented to assess HK-2 and GLS1 expression. **P < 0.01.

3.4 NR2F1-AS1 could sponge miR-363-3p

The mechanism of NR2F1-AS1 was determined via software analysis, with four miRNAs – miR-493-5p, miR-320a, miR-363-3p, and miR-642a-3p – predicted as candidate miRNAs. After silencing of NR2F1-AS1, miRNA expression was tested, and we observed that the expression of miR-363-3p was the highest (Figure 4a and b). Thus, miR-363-3p was chosen for this study. Next, the dual-luciferase reporter data revealed that miR-363-3p mimics remarkably repressed luciferase activity of NR2F1-AS1 WT vector, but it did not affect the NR2F1-AS1 mutant vector (Figure 4c and d). Finally, a lower level of miR-363-3p was found in NSCLC samples (Figure 4e and f), which was the inverse of the expression pattern of NR2F1-AS1. Therefore, NR2F1-AS1 could sponge miR-363-3p expression.

Figure 4 NR2F1-AS1 served as miR-363-3p sponge in NSCLC cells. (a and b) By transfecting with sh-NC or sh-NR2F1-AS1, the expression of miR-493-5p, miR-320a, miR-363-3p, and miR-642a-3p was assessed in A549 and H522 cells by qRT-PCR. (c and d) The connections between miR-363-3p and NR2F1-AS1 were presented by dual-luciferase reporter assay. (e) The level of miR-363-3p in the paired NSCLC samples and adjacent samples was tested by qRT-PCR. (f) The expression of miR-363-3p in BEAS-2B cells and NSCLC cell lines (A549 and H522) was tested by qRT-PCR. **P < 0.01.
Figure 4

NR2F1-AS1 served as miR-363-3p sponge in NSCLC cells. (a and b) By transfecting with sh-NC or sh-NR2F1-AS1, the expression of miR-493-5p, miR-320a, miR-363-3p, and miR-642a-3p was assessed in A549 and H522 cells by qRT-PCR. (c and d) The connections between miR-363-3p and NR2F1-AS1 were presented by dual-luciferase reporter assay. (e) The level of miR-363-3p in the paired NSCLC samples and adjacent samples was tested by qRT-PCR. (f) The expression of miR-363-3p in BEAS-2B cells and NSCLC cell lines (A549 and H522) was tested by qRT-PCR. **P < 0.01.

3.5 NR2F1-AS1 promoted NSCLC progression by working as miR-363-3p sponging

To verify whether NR2F1-AS1 regulates NSCLC progression by modulating miR-363-3p expression, the sh-NR2F1-AS1 and miR-363-3p inhibitors were cotransfected into cells. The cell function analysis demonstrated that miR-363-3p inhibitor attenuated the inhibitory role of NR2F1-AS1 downregulation in the progression of A549 and H522 cells (Figure 5a–f). Furthermore, the repressing effect of sh-NR2F1-AS1 on ECAR, glucose consumption, lactate generation, glutamine consumption, and ATP production of NSCLC cells was abrogated via miR-363-3p inhibitor (Figure 5g–l). So, NR2F1-AS1 promoted NSCLC progression via miR-363-3p sponging.

Figure 5 NR2F1-AS1 promoted NSCLC progression via sponging miR-363-3p. A549 and H522 cells were transfected with sh-NC, sh-NR2F1-AS1, sh-NR2F1-AS1 + anti-miR-NC, or sh-NR2F1-AS1 + anti-miR-363-3p. The (a) viability, (b) colony number, (c and d) migration, and (e and f) invasion of cells were determined using CCK8 assay, colony formation assay, and transwell assay. (g and h) Cell glycolysis, (i) glucose consumption, (j) lactate production, (k) glutamine consumption, and (l) ATP production were evaluated by the corresponding assay, respectively. **P < 0.01 compared with the sh-NC group. ## P < 0.01 compared with sh-NR2F1-AS1 group.
Figure 5

NR2F1-AS1 promoted NSCLC progression via sponging miR-363-3p. A549 and H522 cells were transfected with sh-NC, sh-NR2F1-AS1, sh-NR2F1-AS1 + anti-miR-NC, or sh-NR2F1-AS1 + anti-miR-363-3p. The (a) viability, (b) colony number, (c and d) migration, and (e and f) invasion of cells were determined using CCK8 assay, colony formation assay, and transwell assay. (g and h) Cell glycolysis, (i) glucose consumption, (j) lactate production, (k) glutamine consumption, and (l) ATP production were evaluated by the corresponding assay, respectively. **P < 0.01 compared with the sh-NC group. ## P < 0.01 compared with sh-NR2F1-AS1 group.

3.6 Upregulation of SOX4 reversed the repressing effect of sh-NR2F1-AS1 on NSCLC progression

Further tests revealed that miR-363-3p mimics reduce the luciferase activity of SOX4 3′UTR WT vector (Figure 6a and b). Likewise, a remarkably higher SOX4 mRNA level was found in NSCLC samples (Figure 6c and d).

Figure 6 SOX4 was targeted by miR-363-3p. (a and b) The connections between SOX4 3'UTR and miR-363-3p were exhibited using dual-luciferase reporter assay. (c and d) The mRNA levels of SOX4 in the paired NSCLC samples and adjacent tissues, together with BEAS-2B cells and NSCLC cell lines (A549 and H522), were gaged by qRT-PCR. **P < 0.01.
Figure 6

SOX4 was targeted by miR-363-3p. (a and b) The connections between SOX4 3'UTR and miR-363-3p were exhibited using dual-luciferase reporter assay. (c and d) The mRNA levels of SOX4 in the paired NSCLC samples and adjacent tissues, together with BEAS-2B cells and NSCLC cell lines (A549 and H522), were gaged by qRT-PCR. **P < 0.01.

Afterward, both sh-NR2F1-AS1 and pcDNA-SOX4 plasmid were cotransfected into cells. The viability, colony number migration, and invasion analysis demonstrated that pcDNA-SOX4 attenuates the repression effect of NR2F1-AS1 downregulation in the progression of A549 and H522 cells (Figure 7a–f). Furthermore, the repressing effect of NR2F1-AS1 downregulation on ECAR, glucose consumption, lactate generation, glutamine consumption, and ATP production of NSCLC cells was abrogated by pcDNA-SOX4 (Figure 7g–l). These findings suggest that NR2F1-AS1 modulates the tumorigenic behavior of NSCLC cells via the miR-363-3p/SOX4 axis.

Figure 7 NR2F1-AS1 promoted NSCLC progression by SOX4 overexpression. A549 and H522 cells were transfected with sh-NC, sh-NR2F1-AS1, and sh-NR2F1-AS1 + pcDNA-SOX4. (a) The viability, (b) colony number, (c and d) migration, and (e and f) invasion of cells were determined using CCK8 assay, colony formation assay, and transwell assay. (g and h) Cell glycolysis, (i) glucose consumption, (j) lactate production, (k) glutamine consumption, and (l) ATP production were evaluated by the corresponding assay, respectively. **P < 0.01 compared with the sh-NC group. ## P < 0.01 compared with sh-NR2F1-AS1 group.
Figure 7

NR2F1-AS1 promoted NSCLC progression by SOX4 overexpression. A549 and H522 cells were transfected with sh-NC, sh-NR2F1-AS1, and sh-NR2F1-AS1 + pcDNA-SOX4. (a) The viability, (b) colony number, (c and d) migration, and (e and f) invasion of cells were determined using CCK8 assay, colony formation assay, and transwell assay. (g and h) Cell glycolysis, (i) glucose consumption, (j) lactate production, (k) glutamine consumption, and (l) ATP production were evaluated by the corresponding assay, respectively. **P < 0.01 compared with the sh-NC group. ## P < 0.01 compared with sh-NR2F1-AS1 group.

4 Discussion

Reprogramming of the glucose metabolism has a vital role in tumorigenesis. During the development of tumor, glycolysis is strongly promoted in tumor cells, thereby augmenting cell aggressiveness and metastatic ability. Targeting inhibition of glycolysis is regarded as an effective strategy for the treatment of NSCLC. In this study, we determined the expression profile of NR2F1-AS1 in NSCLC patients and cell line. Furthermore, we found that NR2F1-AS1 regulates NSCLC cell oncogenicity including glycolysis by miR-363-3p/SOX4 axis.

There is increasing evidence that NR2F1-AS1 operates as an oncogene involved in the progression of NSCLC. Moreover, the expression of NR2F1-AS1 is a potential prognostic biomarker in the development of NSCLC. In accordance with this, we found that NR2F1-AS1 was overexpressed in NSCLC samples, and that the silencing of NR2F1-AS1 inhibits the glycolysis in NSCLC cells. Some studies have revealed that the overexpression of NR2F1-AS1 increased glycolysis in hepatocellular carcinoma cells [29]. Similarly, we found that NR2F1-AS1 is connected to the metabolic changes in NSCLC cells. An important finding is that the silencing of NR2F1-AS1 blocked glycolysis and cell progression of NSCLC cells.

Further, we predicted the four targeted miRNAs of NR2F1-AS1. We found that miR-363-3p is inversely related with the expression pattern of NR2F1-AS1. Other studies found that miR-363-3p plays a key role in other cancers, including hepatocellular carcinoma [30,31,32]. Moreover, we found that levels of miR-363-3p were lower in NSCLC cells, and that this was regulated by NR2F1-AS1. Because of the association between glycolysis and levels of miR-363-3p [33], we checked how miR-363-3p regulated glycolysis in NSCLC cells. Unsurprisingly, the miR-363-3p mimic partially blocked glycolysis in NSCLC cells. This implies that miR-363-3p mediates glycolysis in NSCLC cells. We also found that the miR-363-3p inhibitor overturned the repressing role of NR2F1-AS1 downregulation in glycolysis in NSCLC samples. In summary, deficiency of NR2F1-AS1 blocks glycolysis and tumorigenicity in NSCLC cells via miR-363-3p.

SOX4 is known to have oncogenic functions in several tumors, including glioblastoma and hepatocellular carcinoma. Similarly, higher level of SOX4 stimulated cell progression in NSCLC. Our report found that SOX4 was directly regulated by miR-363-3p in NSCLC cells, and that SOX4 was overexpressed in NSCLC samples.

It is well known that lncRNAs modulate the target mRNA expression by binding to miRNA. In this study, we determined whether NR2F1-AS1 affected the miR-615-3p/SOX4 signal in NSCLC cells. Interestingly, SOX4 level was positively associated with level of NR2F1-AS1, whereas it was negatively associated with level of miR-363-3p in NSCLC samples. Moreover, we found that NR2F1-AS1 induces cell progression in NSCLC samples, but these roles were overturned by miR-615-3p. Our findings imply that NR2F1-AS1 may regulate level of SOX4 via sponging miR-363-3p in NSCLC cells. Therefore, the NR2F1-AS1/miR-615-3p/SOX4 signal may be involved in the glycolysis in NSCLC cells.

In summary, this study showed that inhibition of NR2F1-AS1 suppressed glycolysis in NSCLC cells, and the regulation of miR-363-3p/SOX4 plays a role in this. These insights on NR2F1-AS1 could support its use as a potent treatment choice for NSCLC.

  1. Funding information: There is no funding to report.

  2. Authors contributions: LJ, CC, and LB are responsible for the literature review and writing the discussion and introduction of the article. LPH, QYS, and LB are responsible for the bioinformatics analysis, material and methods, and results sections of the manuscript.

  3. Conflict of interest: The authors declare that they have no conflicts of interest.

  4. Data availability statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

[1] Arbour KC, Riely GJ. Systemic therapy for locally advanced and metastatic non-small cell lung cancer: a review. JAMA. 2019;322(8):764–74.10.1001/jama.2019.11058Search in Google Scholar PubMed

[2] Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209–49.10.3322/caac.21660Search in Google Scholar PubMed

[3] Kim YJ, Oremus M, Chen HH, McFarlane T, Shah D, Horton S. Real-world effectiveness of nivolumab in patients with non-small-cell lung cancer: a systematic review and meta-analysis. Future Oncol. 2020;16(27):2045–58.10.2217/fon-2020-0248Search in Google Scholar PubMed

[4] Shi Y, Chen W, Li C, Zhang Y, Bo M, Qi S, et al. Efficacy and safety of first-line treatments with immune checkpoint inhibitors plus chemotherapy for non-squamous non-small cell lung cancer: a meta-analysis and indirect comparison. Ann Palliat Med. 2021;10(3):2766–75.10.21037/apm-20-1498Search in Google Scholar PubMed

[5] Luengo A, Abbott KL, Davidson SM, Hosios AM, Faubert B, Chan SH, et al. Reactive metabolite production is a targetable liability of glycolytic metabolism in lung cancer. Nat Commun. 2019;10(1):5604.10.1038/s41467-019-13419-4Search in Google Scholar PubMed PubMed Central

[6] Lee JS, Lee H, Lee S, Kang JH, Lee SH, Kim SG, et al. Loss of SLC25A11 causes suppression of NSCLC and melanoma tumor formation. EBioMedicine. 2019;40:184–97.10.1016/j.ebiom.2019.01.036Search in Google Scholar PubMed PubMed Central

[7] Wang K, Huang W, Chen R, Lin P, Zhang T, Ni YF, et al. Di-methylation of CD147-K234 promotes the progression of NSCLC by enhancing lactate export. Cell Metab. 2021;33(1):160–73e6.10.1016/j.cmet.2020.12.010Search in Google Scholar PubMed

[8] Zhou H, Feng B, Abudoureyimu M, Lai Y, Lin X, Tian C, et al. The functional role of long non-coding RNAs and their underlying mechanisms in drug resistance of non-small cell lung cancer. Life Sci. 2020;261:118362.10.1016/j.lfs.2020.118362Search in Google Scholar PubMed

[9] Wilusz JE, Sunwoo H, Spector DL. Long noncoding RNAs: functional surprises from the RNA world. Genes Dev. 2009;23(13):1494–504.10.1101/gad.1800909Search in Google Scholar PubMed PubMed Central

[10] Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, et al. The HIF-1alpha antisense long non-coding RNA drives a positive feedback loop of HIF-1alpha mediated transactivation and glycolysis. Nat Commun. 2021;12(1):1341.10.1038/s41467-021-21535-3Search in Google Scholar PubMed PubMed Central

[11] Shi L, Magee P, Fassan M, Sahoo S, Leong HS, Lee D, et al. A KRAS-responsive long non-coding RNA controls microRNA processing. Nat Commun. 2021;12(1):2038.10.1038/s41467-021-22337-3Search in Google Scholar PubMed PubMed Central

[12] Cao T, Jiang Y, Wang Z, Zhang N, Al-Hendy A, Mamillapalli R, et al. H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas. Oncogene. 2019;38(27):5356–66.10.1038/s41388-019-0808-4Search in Google Scholar PubMed PubMed Central

[13] Ye T, Yang X, Liu H, Lv P, Ye Z. Long non-coding RNA BLACAT1 in human cancers. Onco Targets Ther. 2020;13:8263–72.10.2147/OTT.S261461Search in Google Scholar PubMed PubMed Central

[14] Zeng SHG, Xie JH, Zeng QY, Dai SHH, Wang Y, Wan XM, et al. lncRNA PVT1 promotes metastasis of non-small cell lung cancer through EZH2-mediated activation of Hippo/NOTCH1 signaling pathways. Cell J. 2021;23(1):21–31.Search in Google Scholar

[15] Chen D, Li Y, Wang Y, Xu J. LncRNA HOTAIRM1 knockdown inhibits cell glycolysis metabolism and tumor progression by miR-498/ABCE1 axis in non-small cell lung cancer. Genes Genomics. 2021;43(2):183–94.10.1007/s13258-021-01052-9Search in Google Scholar PubMed

[16] Li Y, Shen R, Wang A, Zhao J, Zhou J, Zhang W, et al. Construction of a prognostic immune-related LncRNA risk model for lung adenocarcinoma. Front Cell Dev Biol. 2021;9:648806.10.3389/fcell.2021.648806Search in Google Scholar PubMed PubMed Central

[17] Jiang W, Kai J, Li D, Wei Z, Wang Y, Wang W. lncRNA HOXB-AS3 exacerbates proliferation, migration, and invasion of lung cancer via activating the PI3K-AKT pathway. J Cell Physiol. 2020;235(10):7194–203.10.1002/jcp.29618Search in Google Scholar PubMed

[18] Yuan S, Xiang Y, Guo X, Zhang Y, Li C, Xie W, et al. Circulating long noncoding RNAs act as diagnostic biomarkers in non-small cell lung cancer. Front Oncol. 2020;10:537120.10.3389/fonc.2020.537120Search in Google Scholar PubMed PubMed Central

[19] Ren MM, Xu S, Wei YB, Yang JJ, Yang YN, Sun SS, et al. Roles of HOTAIR in lung cancer susceptibility and prognosis. Mol Genet Genomic Med. 2020;8(7):e1299.10.1002/mgg3.1299Search in Google Scholar PubMed PubMed Central

[20] Zhu Y, Li J, Bo H, He D, Xiao M, Xiang L, et al. LINC00467 is up-regulated by TDG-mediated acetylation in non-small cell lung cancer and promotes tumor progression. Oncogene. 2020;39(38):6071–84.10.1038/s41388-020-01421-wSearch in Google Scholar PubMed

[21] He W, Zhang Y, Xia S. LncRNA NNT-AS1 promotes non-small cell lung cancer progression through regulating miR-22-3p/YAP1 axis. Thorac Cancer. 2020;11(3):549–60.10.1111/1759-7714.13280Search in Google Scholar PubMed PubMed Central

[22] Feng X, Yang S. Long non-coding RNA LINC00243 promotes proliferation and glycolysis in non-small cell lung cancer cells by positively regulating PDK4 through sponging miR-507. Mol Cell Biochem. 2020;463(1–2):127–36.10.1007/s11010-019-03635-3Search in Google Scholar PubMed

[23] Li S, Lin L. Long noncoding RNA MCF2L-AS1 promotes the cancer stem cell-like traits in non-small cell lung cancer cells through regulating miR-873-5p level. Env Toxicol. 2021;36(7):1457–65.10.1002/tox.23142Search in Google Scholar PubMed

[24] Wang X, Yu X, Wei W, Liu Y. Long noncoding RNA MACC1-AS1 promotes the stemness of nonsmall cell lung cancer cells through promoting UPF1-mediated destabilization of LATS1/2. Env Toxicol. 2020;35(9):998–1006.10.1002/tox.22936Search in Google Scholar PubMed

[25] Bai M, Wu ZZ, Huang YL, Ke J, Xu Q, Wang X. STAT3 activates the transcription of lncRNA NR2F1-AS1 to promote the progression of melanoma via regulating miR-493-5p/GOLM1 axis. J Gene Med. 2021;23(7):e3338.10.1002/jgm.3338Search in Google Scholar PubMed

[26] Zhang Q, Li T, Wang Z, Kuang X, Shao N, Lin Y. lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway. J Cell Mol Med. 2020;24(14):8236–47.10.1111/jcmm.15499Search in Google Scholar PubMed PubMed Central

[27] Guo F, Fu Q, Wang Y, Sui G. Long non-coding RNA NR2F1-AS1 promoted proliferation and migration yet suppressed apoptosis of thyroid cancer cells through regulating miRNA-338-3p/CCND1 axis. J Cell Mol Med. 2019;23(9):5907–19.10.1111/jcmm.14386Search in Google Scholar PubMed PubMed Central

[28] Zhang C, Wu S, Song R, Liu C. Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression. Aging (Albany NY). 2020;13(5):7660–75.10.18632/aging.103564Search in Google Scholar PubMed PubMed Central

[29] Li X, Li Y, Bai S, Zhang J, Liu Z, Yang J. NR2F1-AS1/miR-140/HK2 axis regulates hypoxia-induced glycolysis and migration in hepatocellular carcinoma. Cancer Manag Res. 2021;13:427–37.10.2147/CMAR.S266797Search in Google Scholar PubMed PubMed Central

[30] Geng Q, Li Z, Li X, Wu Y, Chen N. LncRNA NORAD, sponging miR-363-3p, promotes invasion and EMT by upregulating PEAK1 and activating the ERK signaling pathway in NSCLC cells. J Bioenerg Biomembr. 2021;53(3):321–32.10.1007/s10863-021-09892-6Search in Google Scholar PubMed

[31] Chang J, Gao F, Chu H, Lou L, Wang H, Chen Y. miR-363-3p inhibits migration, invasion, and epithelial-mesenchymal transition by targeting NEDD9 and SOX4 in non-small-cell lung cancer. J Cell Physiol. 2020;235(2):1808–20.10.1002/jcp.29099Search in Google Scholar PubMed

[32] Lou W, Ding B, Zhong G, Du C, Fan W, Fu P. Dysregulation of pseudogene/lncRNA-hsa-miR-363-3p-SPOCK2 pathway fuels stage progression of ovarian cancer. Aging (Albany NY). 2019;11(23):11416–39.10.18632/aging.102538Search in Google Scholar PubMed PubMed Central

[33] Khuu C, Sehic A, Eide L, Osmundsen H. Anti-proliferative properties of miR-20b and miR-363 from the miR-106a-363 cluster on human carcinoma cells. Microrna. 2016;5(1):19–35.10.2174/2211536605666160322151813Search in Google Scholar PubMed

Received: 2021-07-13
Revised: 2021-11-10
Accepted: 2021-11-12
Published Online: 2021-12-16

© 2022 Luming Jin et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Articles in the same Issue

  1. Research Articles
  2. AMBRA1 attenuates the proliferation of uveal melanoma cells
  3. A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
  4. Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
  5. Effect of gestational diabetes mellitus on lipid profile: A systematic review and meta-analysis
  6. Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4
  7. Promising novel biomarkers and candidate small-molecule drugs for lung adenocarcinoma: Evidence from bioinformatics analysis of high-throughput data
  8. Plasmapheresis: Is it a potential alternative treatment for chronic urticaria?
  9. The biomarkers of key miRNAs and gene targets associated with extranodal NK/T-cell lymphoma
  10. Gene signature to predict prognostic survival of hepatocellular carcinoma
  11. Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
  12. Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?
  13. Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?
  14. Leptin and PCSK9 concentrations are associated with vascular endothelial cytokines in patients with stable coronary heart disease
  15. Pericentric inversion of chromosome 6 and male fertility problems
  16. Staple line reinforcement with nebulized cyanoacrylate glue in laparoscopic sleeve gastrectomy: A propensity score-matched study
  17. Retrospective analysis of crescent score in clinical prognosis of IgA nephropathy
  18. Expression of DNM3 is associated with good outcome in colorectal cancer
  19. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation
  20. CRRT influences PICCO measurements in febrile critically ill patients
  21. SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis
  22. lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells
  23. circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer
  24. Prognostic value of nicotinamide N-methyltransferase in human cancers: Evidence from a meta-analysis and database validation
  25. GPC2 deficiency inhibits cell growth and metastasis in colon adenocarcinoma
  26. A pan-cancer analysis of the oncogenic role of Holliday junction recognition protein in human tumors
  27. Radiation increases COL1A1, COL3A1, and COL1A2 expression in breast cancer
  28. Association between preventable risk factors and metabolic syndrome
  29. miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
  30. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway
  31. Quercitrin protects human bronchial epithelial cells from oxidative damage
  32. Smurf2 suppresses the metastasis of hepatocellular carcinoma via ubiquitin degradation of Smad2
  33. circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury
  34. Sonoclot’s usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study
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  36. Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma
  37. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway
  38. Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network
  39. Evaluation of menstrual irregularities after COVID-19 vaccination: Results of the MECOVAC survey
  40. Histopathologic findings on removed stomach after sleeve gastrectomy. Do they influence the outcome?
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  45. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
  47. hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling
  48. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network
  49. TCD hemodynamics findings in the subacute phase of anterior circulation stroke patients treated with mechanical thrombectomy
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  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
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  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
https://doi.org/10.1515/med-2021-0403
Keywords for this article
non-small cell lung cancer; glycolysis; NR2F1-AS1; miR-363-3p; SOX4
Creative Commons
BY 4.0

Articles in the same Issue

  1. Research Articles
  2. AMBRA1 attenuates the proliferation of uveal melanoma cells
  3. A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
  4. Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
  5. Effect of gestational diabetes mellitus on lipid profile: A systematic review and meta-analysis
  6. Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4
  7. Promising novel biomarkers and candidate small-molecule drugs for lung adenocarcinoma: Evidence from bioinformatics analysis of high-throughput data
  8. Plasmapheresis: Is it a potential alternative treatment for chronic urticaria?
  9. The biomarkers of key miRNAs and gene targets associated with extranodal NK/T-cell lymphoma
  10. Gene signature to predict prognostic survival of hepatocellular carcinoma
  11. Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
  12. Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?
  13. Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?
  14. Leptin and PCSK9 concentrations are associated with vascular endothelial cytokines in patients with stable coronary heart disease
  15. Pericentric inversion of chromosome 6 and male fertility problems
  16. Staple line reinforcement with nebulized cyanoacrylate glue in laparoscopic sleeve gastrectomy: A propensity score-matched study
  17. Retrospective analysis of crescent score in clinical prognosis of IgA nephropathy
  18. Expression of DNM3 is associated with good outcome in colorectal cancer
  19. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation
  20. CRRT influences PICCO measurements in febrile critically ill patients
  21. SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis
  22. lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells
  23. circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer
  24. Prognostic value of nicotinamide N-methyltransferase in human cancers: Evidence from a meta-analysis and database validation
  25. GPC2 deficiency inhibits cell growth and metastasis in colon adenocarcinoma
  26. A pan-cancer analysis of the oncogenic role of Holliday junction recognition protein in human tumors
  27. Radiation increases COL1A1, COL3A1, and COL1A2 expression in breast cancer
  28. Association between preventable risk factors and metabolic syndrome
  29. miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
  30. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway
  31. Quercitrin protects human bronchial epithelial cells from oxidative damage
  32. Smurf2 suppresses the metastasis of hepatocellular carcinoma via ubiquitin degradation of Smad2
  33. circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury
  34. Sonoclot’s usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study
  35. Four drug metabolism-related subgroups of pancreatic adenocarcinoma in prognosis, immune infiltration, and gene mutation
  36. Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma
  37. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway
  38. Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network
  39. Evaluation of menstrual irregularities after COVID-19 vaccination: Results of the MECOVAC survey
  40. Histopathologic findings on removed stomach after sleeve gastrectomy. Do they influence the outcome?
  41. Analysis of the expression and prognostic value of MT1-MMP, β1-integrin and YAP1 in glioma
  42. Optimal diagnosis of the skin cancer using a hybrid deep neural network and grasshopper optimization algorithm
  43. miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells
  44. Clinical value of SIRT1 as a prognostic biomarker in esophageal squamous cell carcinoma, a systematic meta-analysis
  45. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
  47. hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling
  48. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network
  49. TCD hemodynamics findings in the subacute phase of anterior circulation stroke patients treated with mechanical thrombectomy
  50. Development of a risk-stratification scoring system for predicting risk of breast cancer based on non-alcoholic fatty liver disease, non-alcoholic fatty pancreas disease, and uric acid
  51. Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway
  52. circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis
  53. Human amniotic fluid as a source of stem cells
  54. lncRNA NONRATT013819.2 promotes transforming growth factor-β1-induced myofibroblastic transition of hepatic stellate cells by miR24-3p/lox
  55. NORAD modulates miR-30c-5p-LDHA to protect lung endothelial cells damage
  56. Idiopathic pulmonary fibrosis telemedicine management during COVID-19 outbreak
  57. Risk factors for adverse drug reactions associated with clopidogrel therapy
  58. Serum zinc associated with immunity and inflammatory markers in Covid-19
  59. The relationship between night shift work and breast cancer incidence: A systematic review and meta-analysis of observational studies
  60. LncRNA expression in idiopathic achalasia: New insight and preliminary exploration into pathogenesis
  61. Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway
  62. Moscatilin suppresses the inflammation from macrophages and T cells
  63. Zoledronate promotes ECM degradation and apoptosis via Wnt/β-catenin
  64. Epithelial-mesenchymal transition-related genes in coronary artery disease
  65. The effect evaluation of traditional vaginal surgery and transvaginal mesh surgery for severe pelvic organ prolapse: 5 years follow-up
  66. Repeated partial splenic artery embolization for hypersplenism improves platelet count
  67. Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR
  68. Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis
  69. miR-455-5p enhances 5-fluorouracil sensitivity in colorectal cancer cells by targeting PIK3R1 and DEPDC1
  70. The effect of tranexamic acid on the reduction of intraoperative and postoperative blood loss and thromboembolic risk in patients with hip fracture
  71. Isocitrate dehydrogenase 1 mutation in cholangiocarcinoma impairs tumor progression by sensitizing cells to ferroptosis
  72. Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway
  73. A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer
  74. Lidocaine ameliorates chronic constriction injury-induced neuropathic pain through regulating M1/M2 microglia polarization
  75. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model
  76. miR-1273h-5p suppresses CXCL12 expression and inhibits gastric cancer cell invasion and metastasis
  77. Clinical characteristics of pneumonia patients of long course of illness infected with SARS-CoV-2
  78. circRNF20 aggravates the malignancy of retinoblastoma depending on the regulation of miR-132-3p/PAX6 axis
  79. Linezolid for resistant Gram-positive bacterial infections in children under 12 years: A meta-analysis
  80. Rack1 regulates pro-inflammatory cytokines by NF-κB in diabetic nephropathy
  81. Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients
  82. Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China
  83. Let-7i-3p inhibits the cell cycle, proliferation, invasion, and migration of colorectal cancer cells via downregulating CCND1
  84. β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
  85. Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia
  86. In vitro antimicrobial activity of ozonated oil in liposome eyedrop against multidrug-resistant bacteria
  87. Potential biomarkers for inflammatory response in acute lung injury
  88. A low serum uric acid concentration predicts a poor prognosis in adult patients with candidemia
  89. Antitumor activity of recombinant oncolytic vaccinia virus with human IL2
  90. ALKBH5 inhibits TNF-α-induced apoptosis of HUVECs through Bcl-2 pathway
  91. Risk prediction of cardiovascular disease using machine learning classifiers
  92. Value of ultrasonography parameters in diagnosing polycystic ovary syndrome
  93. Bioinformatics analysis reveals three key genes and four survival genes associated with youth-onset NSCLC
  94. Identification of autophagy-related biomarkers in patients with pulmonary arterial hypertension based on bioinformatics analysis
  95. Protective effects of glaucocalyxin A on the airway of asthmatic mice
  96. Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8
  97. Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation
  98. Effectiveness and clinical benefits of new anti-diabetic drugs: A real life experience
  99. Identification of osteoporosis based on gene biomarkers using support vector machine
  100. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis
  101. miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3
  102. Association of ST-T changes with all-cause mortality among patients with peripheral T-cell lymphomas
  103. LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma
  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
  194. A rare case of endometrial polyp complicated with uterine inversion: A case report and clinical management
  195. Spontaneous rupturing of splenic artery aneurysm: Another reason for fatal syncope and shock (Case report and literature review)
  196. Fungal infection mimicking COVID-19 infection – A case report
  197. Concurrent aspergillosis and cystic pulmonary metastases in a patient with tongue squamous cell carcinoma
  198. Paraganglioma-induced inverted takotsubo-like cardiomyopathy leading to cardiogenic shock successfully treated with extracorporeal membrane oxygenation
  199. Lineage switch from lymphoma to myeloid neoplasms: First case series from a single institution
  200. Trismus during tracheal extubation as a complication of general anaesthesia – A case report
  201. Simultaneous treatment of a pubovesical fistula and lymph node metastasis secondary to multimodal treatment for prostate cancer: Case report and review of the literature
  202. Two case reports of skin vasculitis following the COVID-19 immunization
  203. Ureteroiliac fistula after oncological surgery: Case report and review of the literature
  204. Synchronous triple primary malignant tumours in the bladder, prostate, and lung harbouring TP53 and MEK1 mutations accompanied with severe cardiovascular diseases: A case report
  205. Huge mucinous cystic neoplasms with adhesion to the left colon: A case report and literature review
  206. Commentary
  207. Commentary on “Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma”
  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
  215. Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
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