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circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8

  • Wei Wei , Changyong Wang , Lele Wang and Jianfeng Zhang EMAIL logo
Published/Copyright: March 17, 2022
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Open Medicine
From the journal Open Medicine Volume 17 Issue 1

Abstract

High circ_0020123 expression is associated with poor prognosis in patients with non-small cell lung cancer (NSCLC) as previously reported. Whether circ_0020123 also plays an oncogenic role in lung adenocarcinoma (LUAD) is still unknown. Additionally, circ_0020123 is derived from part of exon (1312–3851) from its host gene PDZ domain-containing protein 8 (PDZD8). We hypothesized that circ_0020123 might affect malignant behaviors of LUAD cells by regulating PDZD8. Reverse transcription quantitative polymerase chain reaction revealed that PDZD8 was highly expressed in LUAD tissues and cells. PDZD8 knockdown suppressed LUAD cell proliferation and migration as shown by colony formation assays, Ethynyl deoxyuridine incorporation assays, Transwell assays, and wound healing assays. circ_0020123 was also found to be upregulated in LUAD tissues and cells. Moreover, circ_0020123 positively regulated PDZD8 expression in LUAD cells but exerted no significant effect on the transcriptional level of PDZD8. Mechanistically, circ_0020123 act as a competing endogenous RNA (ceRNA) to interact with miR-1283, thereby releasing the repression on PDZD8. Moreover, PDZD8 overexpression rescued the suppressive effect of circ_0020123 knockdown on LUAD cell proliferation and migration. In conclusion, circ_0020123 interacts with miR-1283 as a ceRNA to regulate PDZD8 expression, thus promoting the proliferation and migration of LUAD cells. The study might provide new biomarkers for future LUAD investigation.

Keywords: lung adenocarcinoma; PDZD8; circ_0020123; miR-1283; ceRNA

1 Introduction

Lung cancer is a common malignancy with high mortality rate worldwide [1]. There were approximately 1,761,007 lung cancer-related deaths in 2018 [2]. Lung adenocarcinoma (LUAD) is a common subtype of non-small cell lung cancer (NSCLC), accounting for more than 40% of all lung cancer cases [3]. Molecular target therapy and chemotherapy for NSCLC have achieved great progress in recent years, but the 5-year survival rate of NSCLC is no more than 15% [4,5,6]. Therefore, exploring more biomarkers for NSCLC and understanding the underlying mechanisms of NSCLC pathogenesis are important for improving prognosis of NSCLC patients.

We previously found that high circ_0020123 expression is associated with poor prognosis in patients with NSCLC [7]. Herein we aimed to further explore the underlying mechanism of circ_0020123 in LUAD. Since circ_0020123 is cyclized and derived from PDZ domain-containing protein 8 (PDZD8), the functions of circ_0020123 and PDZD8 as well as their relationship in LUAD cells were investigated in the current study.

Circular RNAs (circRNAs) are single stranded RNAs with closed loop structure that have higher cellular stability than long noncoding RNAs [2]. Circular RNAs participate in the development of various cancers by regulating gene expression and interfering biological behaviors of cancer cells [8,9]. Mechanistically, circRNAs can serve as competing endogenous RNAs (ceRNAs) to release downstream messenger RNAs (mRNAs) from the repression exerted by microRNAs. In the ceRNA pattern, circRNAs regulate mRNA expression by interacting with miRNAs [10]. In recent years, many circRNAs including circ_002178 [11], circ_0013958 [12], and circ_006427 [13] have been reported to participate in the development of LUAD by serving as ceRNAs. Circ_0020123 is highly expressed in NSCLC tissues and cells according to our previous study [7]. Moreover, circ_0020123 was reported to interact with miR-144 as a ceRNA to upregulate the expression of zinc finger E-box-binding homeobox 1 (ZEB1) and enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), thereby facilitating NSCLC progression [14]. In addition, circ_0020123 promotes NSCLC development by serving as a ceRNA of miR-488-3p to upregulate ADAM9 expression [15]. We hypothesized that circ_0020123 might function as a ceRNA to modulate its host gene PDZD8.

In the present study, we investigated the role of circ_0020123 and PDZD8 as well as their relationship in LUAD cells. This study may provide new insight into the role of circ_0020123 in LUAD diagnosis and progression.

2 Materials and methods

2.1 Bioinformatics analysis

According to circBase (http://circbase.org/), circ_0020123 is spliced from PDZD8. The genome length of circ_0020123 is 7,254 bp and the spliced length is 2,540 bp [16]. Circular RNA Interactome (CircInteractome) was employed to predict possible miRNAs binding with circ_0020123 [17]. Possible miRNAs interacting with PDZD8 were predicted with miRDB (http://mirdb.org/) [18].

2.2 Patients and tissue specimens

Sixteen fresh LUAD tissues and paired adjacent non-tumor lung tissues were collected from patients with LUAD at Jinling Hospital (Jiansu, China). Before operation, patients had not received any chemotherapy or radiotherapy, and the informed patient consent had been obtained. The pathological and histological diagnostics of LUAD patients were evaluated following the Revised International System for Staging Lung Cancer. The samples were preserved at −80°C before RNA extraction. The Ethics Committee in Jinling Hospital (Jiansu, China) approved the investigation.

2.3 Cell culture

The normal human bronchial cell line BEAS-2B and LUAD cell lines (A549 and H1975) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were incubated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum, 100 IU/mL of 10% penicillin, and 100 µg/mL streptomycin (Invitrogen, Carlsbad, CA, USA). Cell culture was performed in a humidified atmosphere with 5% CO2 at 37°C.

2.4 Cell transfection

Short hairpin RNAs targeting PDZD8 (sh-PDZD8#1/2) or spliced form of hsa_circ_0020123 (sh-circ_0020123#1/2), miR-495 mimics, and their negative controls (sh-NC and NC mimics) were synthesized by Sangon Biotech (Shanghai, China). Empty pcDNA3.1 vectors and pcDNA3.1 vectors overexpressing PDZD8 or circ_0020123 were purchased from GenePharma (Shanghai, China). The above shRNAs (40 nM), mimics (50 nM), and vectors (10 nM) were transfected into A549 and H1975 cells using the Lipofectamine 2000 reagent (Invitrogen) according to the instructions. The transfection efficiency was examined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) after 48 h.

2.5 RT-qPCR

TRIzol reagent (Invitrogen) was utilized to extract RNAs from A549 and H1975 cells, and a PrimeScript First Strand cDNA Synthesis Kit (Aidlab, Beijing, China) was applied to synthesize cDNA. RT-qPCR was performed using SYBR Green qPCR Mix (Invitrogen) with an ABI system (Thermo Fisher Scientific, Waltham, USA). Gene expression was calculated using the 2−∆∆Ct method. GAPDH was an internal control for circ_0020123 and PDZD8, while U6 snRNA was a control set for miRNAs. Sequences of primers were presented as follows:

circ_0023123

Forward: 5′-CTTCTCCAGTTACTTGCTTGTGTAAG-3′

Reverse: 5′-GTATCTACTGTCAACCCGGCAG-3′

PDZD8

Forward: 5′-CTACCGAATTACAAGATCAGGT-3′

Reverse: 5′-ACGAAGTGTAAGTCCAACAC-3′

miR-1283

Forward: 5′-TCTACAAAGGAAAGCGCTTTCT-3′

Reverse: 5′-CTCTACAGCTATATTGCCAGCCA-3′

miR-548c-3p

Forward: 5′-CAAAAATCTCAATTACTTTTGC-3′

Reverse: 5′-CTCTACAGCTATATTGCCAGC-3′

GAPDH

Forward: 5′-TCATTTCCTGGTATGACAACGA-3′

Reverse: 5′-GGTCTTACTCCTTGGAGGC-3′

U6

Forward: 5′-GGATCAATACAGAGCAGATAAGC-3′

Reverse: 5′-CTTTCTGAATTTGCGTGCC-3′.

2.6 Western blot analysis

After transfection, A549 and H1975 cells were lysed with RIPA lysis buffer containing protease inhibitor. Protein concentration was determined by BCA method. Next extracted protein was subjected to 10% sodium dodecyl sulfate polyacrylamide electrophoresis gel, transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA), and then was blocked in nonfat milk at room temperature for 2 h. Afterwards, the membrane was incubated with primary antibodies of anti-PDZD8 (PA5-46771; 1:1,000; Thermo Fisher) and anti-GAPDH (ab125247; 1:2,000; Abcam, Cambridge, MA, USA) at 4°C overnight. Subsequently, the membrane was washed and incubated with secondary antibody (Abcam) at room temperature for 2 h. The protein bands were visualized by the enhanced chemiluminescence detection system (Thermo Fisher Scientific) and analyzed by ImageJ software (National Institutes of Health, Bethesda, MA, USA). GAPDH was set as a loading control.

2.7 Subcellular fractionation assay

The nuclear and cytoplasmic portion of LUAD cells were extracted using a PARIS Kit (Thermo Fisher Scientific) according to instructions. Briefly, cells were incubated with lysis solution for 10 min on ice and then was centrifuged at 12,000g for 3 min. Cytoplasmic RNA was extracted from the supernatant and nuclear RNA was collected from nuclear pellet. The expression levels of PDZD8, GAPDH, and U6 in the cytoplasm and nuclear components were examined by RT-qPCR. GAPDH served as a cytoplasmic control, while U6 acts as a nuclear control.

2.8 Colony formation assay

After transfection, A549 and H1975 cells were seeded into 6-well plates (500 cells/well) and cultured in DMEM for 2 weeks. The medium was changed every 2 days. After incubation, the colonies were fixed with methanol for 30 min and stained with 3% crystal violet solution (Dingguo Biotech, Shanghai, China) for 20 min. Finally, images were taken, and the number of viable colonies (more than 50 cells per colony) was counted.

2.9 5-Ethynyl-2′-deoxyuridine (EdU) assay

An EdU kit (Roche, Indianapolis, IN, USA) was used to conduct the EdU assay. After indicated transfection, A549 and H1975 cells (5 × 104) were incubated in DMEM with EdU solution (RiboBio) at 37°C for 4 h. Next the cells were fixed with 4% formaldehyde for 30 min and treated with glycine for 5 min. Subsequently, LUAD cells were subject to 0.5% Triton X-100 at 28°C for 10 min to make the cell membranes permeable. After being washed with phosphate buffer solution, each well was incubated with Apollo reaction cocktail for 30 min. Finally, DAPI was used to stain the nuclear DNA, and images were captured using a fluorescence microscopy (Olympus, Tokyo, Japan).

2.10 Transwell assay

To assess the migratory capacity of LUAD cells, 24-well Transwell chambers (BD Biosciences, Franklin Lakes, NJ, USA) were utilized. The LUAD cells (5 × 104) suspended in serum-free DMEM were added to the upper chamber, and the lower chamber was supplemented with 600 µL of DMEM containing 20% fetal bovine serum. Next both the upper and lower chambers were incubated for 48 h with 5% CO2 and 95% humidity at 37°C. The cells on the surface of the upper chambers were carefully removed with a cotton swab. Then, the migrated cells were fixed with 10% formaldehyde and stained with 0.5% crystal violet (Beyotime) for 25 min at 24°C. The cells in 5 randomly selected views were counted under an inverted microscope (Olympus) at 200× magnification.

2.11 Wound healing assay

The indicated cells were cultured in 6-well plates. A sterile pipette tip was utilized to make wounds in each monolayer of cells when the cells were approximately 90% confluent. Next PBS was utilized to wash away cell debris and fresh medium was added. The distance between the two edges of the wound was measured. After 24 h, microscopic images were taken at the same field. Wound closure rate was calculated according to the formula: (the original wound areas – the actual wound areas at 24 h)/(the original wound areas).

2.12 Agarose gel electrophoresis

A DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) was utilized to extract genomic DNA (gDNA) from LUAD cells according to the instructions. TRIzol reagent (Invitrogen) was applied to extract total RNA from LUAD cells. RNA degradation and contamination were examined on 1% agarose gels.

2.13 RNase R treatment

The total RNA (5 µg) was incubated with or without 3 U/µg of RNase R (Epicenter Biotechnologies, WI, USA) at 37°C for 30 min. Afterwards, the RNA was purified using RNeasy MinElute Cleanup Kit (QIAGEN). At last, RNA was transcribed into cDNA, and the expression levels of PDZD8 and circ_0020123 were assessed by RT-qPCR analysis.

2.14 RNA immunoprecipitation (RIP) assay

An EZ-Magna RIP Kit (Millipore, Bedford, MA, USA) was utilized for the RIP assay. A549 and H1975 cells were lysed in a RIP lysis buffer. Next the cell lysate was incubated overnight at 4°C with protein G Sepharose beads precoated with Argonaute2 (Ago2) antibody. IgG antibody was set as the negative control. After the beads were washed, TRIzol reagent was utilized to extract the RNAs. At last, RT-qPCR was performed to evaluate the expression levels of the immunoprecipitated RNAs.

2.15 Luciferase reporter assay

The binding area between circ_0020123 and miR-1283 was predicted with CircInteractome, while that between miR-1283 and PDZD8 was predicted using miRDB. The wild-type (Wt) and mutated (Mut) fragments of miR-1283 were synthesized and, respectively, subcloned into pmirGLO vectors (Promega, Madison, WI, USA) to construct pmirGIO-miR-1283-Wt/Mut vectors. The Wt and Mut sequence of PDZD8 3′-untranslated region (UTR) containing predicted binding site with miR-1283 were subcloned into pmirGLO vectors to construct pmirGIO-PDZD8-Wt/Mut vectors. Next sh-circ_0020123#1 or sh-NC were cotransfected with miR-1283-Wt/Mut vectors into A549 and H1975 cells while PDZD8-Wt/Mut vectors were cotransfected with NC mimics, miR-1283 mimics, or miR-1283 + pcDNA3.1/circ_0020123 into LUAD cells using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s recommendations. The luciferase activities of miR-1283-Wt/Mut and PDZD8-Wt/Mut were assessed using a Dual Luciferase Reporter Assay Kit (Promega) after 48 h cotransfection.

2.16 RNA pulldown assay

Biotinylated miR-1283 WT/MUT and biotinylated negative control (bio-NC) were purchased from Sangon (Shanghai, China) and were transfected into LUAD cells. After 24 h transfection, LUAD cells were lysed as described above. Next streptavidin agarose beads (Invitrogen) were added to cell lysate for 10 min. The RNA bound to the beads were quantified by RT-qPCR.

2.17 Statistical analysis

Statistical analysis was performed using SPSS 19.0 software (Chicago, IL, USA). Each experiment was conducted at least three times, and data are shown as the mean value ± standard deviation. Differences between two groups were compared using Student’s t test, while that among more groups were analyzed by One-way analysis of variance followed by Tukey’s post hoc test. Spearman correlation coefficient was employed to identify gene expression correlation in LUAD tissues. The values of p < 0.05 were considered as statistically significant.

3 Results

3.1 PDZD8 is highly expressed in LUAD tissues and cells

We first explored the expression of PDZD8 in LUAD tissues using RT-qPCR analysis. Compared with its expression in normal tissues, PDZD8 expression was significantly increased in LUAD tissues (Figure 1a). Next RT-qPCR and western blot analyses were performed to examine PDZD8 mRNA expression and protein levels in LUAD cells (A549 and H1975) and normal human bronchial epithelial cells (BEAS-2B). Both mRNA and protein levels of PDZD8 were much higher in LUAD cells than in normal bronchial cells (Figure 1b). Afterwards, the localization of PDZD8 in LUAD cells was explored by subcellular fractionation assays. The results revealed that PDZD8 was mainly distributed in the cytoplasm (Figure 1c).

Figure 1 PDZD8 is highly expressed in LUAD tissues and cells. (a) PDZD8 expression in LUAD tissues (n = 16) was elevated compared with that in normal tissues (n = 16) as shown by RT-qPCR. **p < 0.01. (b) The mRNA and protein levels of PDZD8 in LUAD cells were increased as suggested by RT-qPCR and western blot analyses. **p < 0.01, ***p < 0.001 vs BEAS-2B group. (c) Subcellular fractionation assays revealed that PDZD8 was mainly distributed in cytoplasm of LUAD cells. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.
Figure 1

PDZD8 is highly expressed in LUAD tissues and cells. (a) PDZD8 expression in LUAD tissues (n = 16) was elevated compared with that in normal tissues (n = 16) as shown by RT-qPCR. **p < 0.01. (b) The mRNA and protein levels of PDZD8 in LUAD cells were increased as suggested by RT-qPCR and western blot analyses. **p < 0.01, ***p < 0.001 vs BEAS-2B group. (c) Subcellular fractionation assays revealed that PDZD8 was mainly distributed in cytoplasm of LUAD cells. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.

3.2 PDZD8 knockdown inhibits the proliferation and migration of LUAD cells

A series of loss-of-function assays were performed to explore the impact of PDZD8 knockdown on LUAD cell proliferation and migration. The knockdown efficiency of sh-PDZD8#1/2 was examined using RT-qPCR and western blot, and the results showed that PDZD8 was markedly decreased by sh-PDZD8#1/2 in A549 and H1975 cells (Figure 2a). Colony formation assays and EdU assays revealed that PDZD8 knockdown decreased the number of cell colonies and EdU positive cells, which indicated that PDZD8 knockdown suppressed the proliferation of LUAD cells (Figure 2b and c). As suggested by Transwell assays, the number of migrated cells was greatly reduced by PDZD8 depletion (Figure 2d). Wound healing assays showed that the wound closure rate in sh-PDZD8#1/2 group was decreased compared with that in sh-NC group (Figure 2e). The results implied that PDZD8 knockdown decreased the migratory capacity of LUAD cells.

Figure 2 PDZD8 knockdown inhibits proliferation and migration of LUAD cells. (a) PDZD8 mRNA expression and protein levels were decreased by sh-PDZD8#1/2 in A549 and H1975 cells according to RT-qPCR and western blot. (b and c) Colony formation assays and EdU assays suggested the suppressive effect of PDZD8 knockdown on LUAD cell proliferation. (d and e) Transwell and wound healing assays revealed that PDZD8 silencing inhibited the migration of LUAD cells. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups. ***p < 0.001 vs sh-NC group.
Figure 2 PDZD8 knockdown inhibits proliferation and migration of LUAD cells. (a) PDZD8 mRNA expression and protein levels were decreased by sh-PDZD8#1/2 in A549 and H1975 cells according to RT-qPCR and western blot. (b and c) Colony formation assays and EdU assays suggested the suppressive effect of PDZD8 knockdown on LUAD cell proliferation. (d and e) Transwell and wound healing assays revealed that PDZD8 silencing inhibited the migration of LUAD cells. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups. ***p < 0.001 vs sh-NC group.
Figure 2

PDZD8 knockdown inhibits proliferation and migration of LUAD cells. (a) PDZD8 mRNA expression and protein levels were decreased by sh-PDZD8#1/2 in A549 and H1975 cells according to RT-qPCR and western blot. (b and c) Colony formation assays and EdU assays suggested the suppressive effect of PDZD8 knockdown on LUAD cell proliferation. (d and e) Transwell and wound healing assays revealed that PDZD8 silencing inhibited the migration of LUAD cells. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups. ***p < 0.001 vs sh-NC group.

3.3 circ_0020123 positively regulates PDZD8 expression in LUAD tissues and cells

As suggested by RT-qPCR analysis, circ_0020123 showed higher expression in LUAD tissues than in non-tumor tissues (Figure 3a). In addition, high expression of circ_0020123 was also examined in A549 and H1975 cells compared with its expression in normal bronchial cell line BEAS-2B (Figure 3b). Subsequently, we probed the correlation between circ_0020123 and PDZD8. Based on Spearman correlation coefficient analysis, circ_0020123 expression was positively correlated with PDZD8 expression in LUAD tissues (Figure 3c). Next we knocked down circ_0020123 expression in A549 and H1975 cells via sh-circ_0020123#1/2 transfection to explore whether PDZD8 expression would be affected. The plasmid sh-circ_0020123#1/2 was designed to target the sequence around backsplice junction site. In other words, the sh-circ_0020123#1/2 targets the spliced circ_0020123 form and silences only circ_0020123. RT-qPCR revealed that circ_0020123 expression was successfully decreased with sh-circ_0020123#1/2 (Figure 3d). Western blot and RT-qPCR analyses revealed that both mRNA and protein levels of PDZD8 were downregulated by circ_0020123 knockdown in A549 and H1975 cells (Figure 3e). Next circ_0020123 expression was significantly upregulated in LUAD cells after transfection of pcDNA3.1/circ_0020123 (Figure 3f). Overexpressed circ_0020123 increased mRNA expression and protein levels of PDZD8 in LUAD cells (Figure 3g). However, silenced PDZD8 had no significant effect on circ_0020123 expression in A549 and H1975 cells (Figure 3h). Luciferase reporter assays were carried out to determine the impact of circ_0020123 on the transcription level of PDZD8. We found that the luciferase activity of PDZD8 promoter showed no response to circ_0020123 knockdown or overexpression (Figure 3i). RIP assays were conducted to further explore the relationship between circ_0020123 and PDZD8. The significant enrichment of circ_0020123 and PDZD8 in anti-Ago2 group was identified in A549 and H1975 cells, suggesting that circ_0020123 and PDZD8 were enriched in RNA-induced silencing complex (Figure 3j). Subsequently, we explored the existence of circ_0020123 in LUAD cells. After RNase R treatment, circ_0020123 was resistant to RNase R, while linear PDZD8 expression was markedly downregulated, which implied that circ_0020123 isoform was more stable than the linear PDZD8 (Figure 3k). Convergent and divergent primers were designed to amplify the linear and backsplicing forms of PDZD8 using cDNA and gDNA as the templates. The agarose gel electrophoresis showed that circ_0020123 was amplified by cDNA templates, while PDZD8 was amplified by both cDNA and gDNA templates (Figure 3l).

Figure 3 circ_0020123 positively regulates PDZD8 expression in LUAD tissues and cells. (a) circ_0020123 expression was upregulated in LUAD tissues as detected by RT-qPCR. **p < 0.01. (b) circ_0020123 showed high expression in LUAD cells as shown by RT-qPCR. ***p < 0.001 vs BEAS-2B group. (c) A positive expression correlation between circ_0020123 and PDZD8 in LUAD tissues was identified using Spearman correlation coefficient. R 2 = 0.405, *p < 0.05. (d) circ_0020123 expression was successfully knocked down in LUAD cells as shown by RT-qPCR. ***p < 0.001 vs sh-NC group. (e) circ_0020123 knockdown reduced PDZD8 expression at both the mRNA and protein levels. ***p < 0.001 vs sh-NC group. (f) RT-qPCR was performed to detect the efficiency of circ_0020123 overexpression. ***p < 0.001 vs pcDNA3.1 group. (g) The influence of overexpressed circ_0020123 on PDZD8 mRNA expression and protein levels in LUAD cells was quantified by RT-qPCR and western blot analyses. ***p < 0.001 vs pcDNA3.1 group. (h) The effect of PDZD8 knockdown on circ_0020123 expression in LUAD cells were examined by RT-qPCR. (i) Luciferase reporter assays were conducted to explore the effect of circ_0020123 on the transcription level of PDZD8. (j) RIP assays implied that circ_0020123 and PDZD8 were enriched in RNA-induced silencing complex. ***p < 0.001 vs Anti-IgG group. (k) The expression levels of circ_0020123 and linear PDZD8 in A549 and H1975 cells after RNase R treatment were determined by RT-qPCR analysis. ***p < 0.001 vs Mock group. (l) Agarose gel electrophoresis showed that circ_0020123 can be amplified by divergent primers using total cDNA, but not genomic DNA (gDNA). Pair triangles indicate the directionality of primers. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.
Figure 3

circ_0020123 positively regulates PDZD8 expression in LUAD tissues and cells. (a) circ_0020123 expression was upregulated in LUAD tissues as detected by RT-qPCR. **p < 0.01. (b) circ_0020123 showed high expression in LUAD cells as shown by RT-qPCR. ***p < 0.001 vs BEAS-2B group. (c) A positive expression correlation between circ_0020123 and PDZD8 in LUAD tissues was identified using Spearman correlation coefficient. R 2 = 0.405, *p < 0.05. (d) circ_0020123 expression was successfully knocked down in LUAD cells as shown by RT-qPCR. ***p < 0.001 vs sh-NC group. (e) circ_0020123 knockdown reduced PDZD8 expression at both the mRNA and protein levels. ***p < 0.001 vs sh-NC group. (f) RT-qPCR was performed to detect the efficiency of circ_0020123 overexpression. ***p < 0.001 vs pcDNA3.1 group. (g) The influence of overexpressed circ_0020123 on PDZD8 mRNA expression and protein levels in LUAD cells was quantified by RT-qPCR and western blot analyses. ***p < 0.001 vs pcDNA3.1 group. (h) The effect of PDZD8 knockdown on circ_0020123 expression in LUAD cells were examined by RT-qPCR. (i) Luciferase reporter assays were conducted to explore the effect of circ_0020123 on the transcription level of PDZD8. (j) RIP assays implied that circ_0020123 and PDZD8 were enriched in RNA-induced silencing complex. ***p < 0.001 vs Anti-IgG group. (k) The expression levels of circ_0020123 and linear PDZD8 in A549 and H1975 cells after RNase R treatment were determined by RT-qPCR analysis. ***p < 0.001 vs Mock group. (l) Agarose gel electrophoresis showed that circ_0020123 can be amplified by divergent primers using total cDNA, but not genomic DNA (gDNA). Pair triangles indicate the directionality of primers. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.

3.4 circ_0020123 acts as a ceRNA against miR-1283 to regulate PDZD8

To search possible miRNAs that interact with both circ_0020123 and PDZD8, CircInteractome was employed to predict miRNAs binding to circ_0020123, while miRDB was utilized to predict miRNAs interacting with PDZD8. As shown in the Venn diagram, four candidate miRNAs (miR-513a-3p, miR-590-5p, miR-548c-3p, and miR-1283) were identified (Figure 4a). In previous studies, functions of miR-513a-3p and miR-590-5p in LUAD have been investigated [19,20]. Thus, we examined miR-548c-3p and miR-1283 expression levels in A549 and H1975 cells with transfection of sh-circ_0020123#1/2. RT-qPCR suggested that miR-1283 expression was significantly increased by silencing circ_0020123, while miR-548c-3p expression was not significantly affected by circ_0020123 depletion in LUAD cells (Figure 4b). Next we detected miR-1283 expression in LUAD tissues using RT-qPCR, which suggested that miR-1283 was downregulated in LUAD tissues compared with that in normal tissues (Figure 4c). According to Spearman correlation coefficient, miR-1283 expression was negatively correlated with PDZD8 expression (or circ_0020123 expression) in LUAD tissues (Figure 4d). Next miR-1283 was overexpressed with miR-1283 mimics, and the overexpression efficiency was examined by RT-qPCR (Figure 4e). In western blot and RT-qPCR analyses, PDZD8 expression was markedly decreased at both mRNA and protein levels due to miR-1283 overexpression in LUAD cells (Figure 4f). To explore the relationship among circ_0020123, miR-1283, and PDZD8, luciferase reporter assays, RNA pulldown assays, and RIP assays were carried out. The possible binding site between circ_0020123 and miR-1283 was predicted from CircInteractome, while the binding site between miR-1283 and PDZD8 was predicted from miRDB (Figure 4g). The luciferase activity of miR-1283-Wt was significantly reduced by circ_0020123 overexpression, while no significant changes were detected in the miR-1283-Mut group in LUAD cells (Figure 4h). The luciferase activity of PDZD8-Wt was markedly downregulated due to miR-1283 overexpression and the decrease was rescued by overexpression of circ_0020123, but no significant changes happened in the activity of PDZD8-Mut (Figure 4i). The results demonstrated that circ_0020123 interacted with miR-1283 as a ceRNA to upregulate PDZD8. RNA pulldown assays were carried out to further probe the interaction between circ_0020123 and miR-1283. circ_0020123 was abundantly enriched in biotinylated miR-1283 WT group compared with its enrichment in the control Mut group (Figure 4j). The results further confirmed the direct interaction between circ_0020123 and PDZD8. Moreover, circ_0020123, miR-1283, and PDZD8 expression levels were all significantly upregulated in anti-Ago2 groups, indicating that circ_0020123, miR-1283, and PDZD8 were enriched in the RNA-induced silencing complex (Figure 4k).

Figure 4 circ_0020123 acts as a ceRNA against miR-1283 to regulate PDZD8. (a) CircInteractome and miRDB databases were utilized to predict miRNAs binding with circ_0020123 and PDZD8, respectively. (b) circ_0020123 knockdown upregulated miR-1283 expression and exert no significant effect on miR-548c-3p level in LUAD cells. **p < 0.01 vs sh-NC group. (c) MiR-1283 expression in LUAD tissues was relatively low. ***p < 0.001. (d) Based on Spearman correlation coefficient, miR-1283 expression was negatively correlated with circ_0020123 expression (R 2 = 0.269, *p < 0.05) and PDZD8 expression (R 2 = 0.433, *p < 0.05) in LUAD tissues. (e) miR-1283 expression was successfully overexpressed by miR-1283 mimics in LUAD cells. ***p < 0.001 vs NC mimics group. (f) miR-1283 overexpression decreased mRNA expression and protein levels of PDZD8 in A549 and H1975 cells. ***p < 0.001 vs NC mimics group. (g) The possible binding site between miR-1283 and circ_0020123 (or PDZD8) was predicted using CircInteractome and miRDB, respectively. (h) Luciferase reporter assays indicated the binding capacities between miR-1283 and circ_0020123. ***p < 0.001 vs sh-NC group. (i) The relationship of circ_0020123, miR-1283, and PDZD8 in LUAD cells has been verified by luciferase reporter assays. ***p < 0.001 vs NC mimics group or miR-1283 mimics group. (j) The direct interaction between circ_0020123 and miR-1283 was further confirmed by RNA pulldown assays. ***p < 0.001 vs bio-NC group. (k) The enrichment of circ_0020123, miR-1283, and PDZD8 in Ago2 and IgG antibodies was probed by RIP assays. ***p < 0.001 vs Anti-IgG group. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.
Figure 4

circ_0020123 acts as a ceRNA against miR-1283 to regulate PDZD8. (a) CircInteractome and miRDB databases were utilized to predict miRNAs binding with circ_0020123 and PDZD8, respectively. (b) circ_0020123 knockdown upregulated miR-1283 expression and exert no significant effect on miR-548c-3p level in LUAD cells. **p < 0.01 vs sh-NC group. (c) MiR-1283 expression in LUAD tissues was relatively low. ***p < 0.001. (d) Based on Spearman correlation coefficient, miR-1283 expression was negatively correlated with circ_0020123 expression (R 2 = 0.269, *p < 0.05) and PDZD8 expression (R 2 = 0.433, *p < 0.05) in LUAD tissues. (e) miR-1283 expression was successfully overexpressed by miR-1283 mimics in LUAD cells. ***p < 0.001 vs NC mimics group. (f) miR-1283 overexpression decreased mRNA expression and protein levels of PDZD8 in A549 and H1975 cells. ***p < 0.001 vs NC mimics group. (g) The possible binding site between miR-1283 and circ_0020123 (or PDZD8) was predicted using CircInteractome and miRDB, respectively. (h) Luciferase reporter assays indicated the binding capacities between miR-1283 and circ_0020123. ***p < 0.001 vs sh-NC group. (i) The relationship of circ_0020123, miR-1283, and PDZD8 in LUAD cells has been verified by luciferase reporter assays. ***p < 0.001 vs NC mimics group or miR-1283 mimics group. (j) The direct interaction between circ_0020123 and miR-1283 was further confirmed by RNA pulldown assays. ***p < 0.001 vs bio-NC group. (k) The enrichment of circ_0020123, miR-1283, and PDZD8 in Ago2 and IgG antibodies was probed by RIP assays. ***p < 0.001 vs Anti-IgG group. The data are presented as the mean value ± standard deviation. Student’s t test was used to compare differences between LUAD tissues and normal tissues. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.

3.5 PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration

Rescue assays were performed to determine whether circ_0020123 promotes cellular behaviors by regulating its host gene PDZD8. As shown by RT-qPCR and western blot, PDZD8 mRNA and protein levels were significantly increased after transfection of pcDNA3.1/PDZD8 (Figure 5a). Colony formation and EdU assays showed that silencing circ_0020123 inhibited LUAD cell proliferation and PDZD8 overexpression rescued the suppressive effect on cell proliferation induced by circ_0020123 knockdown (Figure 5b and c). According to Transwell and wound healing assays, circ_0020123 depletion suppressed the migration of LUAD cells and PDZD8 overexpression reversed the decrease in migratory ability induced by circ_0020123 silencing (Figure 5d and e). As shown by Figure 5f, circ_0020123 is spliced from PDZD8 and is highly expressed in LUAD cells. circ_0020123 directly interacts with miR-1283 to indirectly upregulate PDZD8. MiR-1283 is downregulated in LUAD cells and directly bind to PDZD8. In summary, circ_0020123 competes with PDZD8 for the chance of binding with miR-1283, thereby releasing PDZD8 from degradation and thus promoting cell proliferation and migration.

Figure 5 PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration. (a) The mRNA expression and protein levels of PDZD8 were increased after transfection of pcDNA3.1/PDZD8 into LUAD cells. **p < 0.01, ***p < 0.001 vs pcDNA3.1 group. (b and c) Colony formation assays and EdU assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (d) Transwell assays showed that the suppressive effect of circ_0020123 depletions on LUAD cell migration was reversed by PDZD8 overexpression. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (e) Wound healing assays were performed to detect the migration of LUAD cells transfected with sh-NC, sh-circ_0020123#1, or sh-circ_0020123#1 + PDZD8. ***p < 0.001 vs sh-NC group or sh-circ_0020123#1 group. (f) The relationship of circ_0020123, miR-1283, and PDZD8 as well as their roles in LUAD cells has been illustrated by a schematic diagram. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.
Figure 5 PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration. (a) The mRNA expression and protein levels of PDZD8 were increased after transfection of pcDNA3.1/PDZD8 into LUAD cells. **p < 0.01, ***p < 0.001 vs pcDNA3.1 group. (b and c) Colony formation assays and EdU assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (d) Transwell assays showed that the suppressive effect of circ_0020123 depletions on LUAD cell migration was reversed by PDZD8 overexpression. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (e) Wound healing assays were performed to detect the migration of LUAD cells transfected with sh-NC, sh-circ_0020123#1, or sh-circ_0020123#1 + PDZD8. ***p < 0.001 vs sh-NC group or sh-circ_0020123#1 group. (f) The relationship of circ_0020123, miR-1283, and PDZD8 as well as their roles in LUAD cells has been illustrated by a schematic diagram. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.
Figure 5

PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration. (a) The mRNA expression and protein levels of PDZD8 were increased after transfection of pcDNA3.1/PDZD8 into LUAD cells. **p < 0.01, ***p < 0.001 vs pcDNA3.1 group. (b and c) Colony formation assays and EdU assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (d) Transwell assays showed that the suppressive effect of circ_0020123 depletions on LUAD cell migration was reversed by PDZD8 overexpression. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (e) Wound healing assays were performed to detect the migration of LUAD cells transfected with sh-NC, sh-circ_0020123#1, or sh-circ_0020123#1 + PDZD8. ***p < 0.001 vs sh-NC group or sh-circ_0020123#1 group. (f) The relationship of circ_0020123, miR-1283, and PDZD8 as well as their roles in LUAD cells has been illustrated by a schematic diagram. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.

4 Discussion

LUAD is a common malignancy worldwide and its therapeutic effect still needs to be improved due to the lack of effective diagnostic and therapeutic methods [11]. CircRNA is a kind of noncoding RNA with neither a free 3′ nor 5′ end [11,21]. CircRNAs play a vital role in the progression of various cancers, including LUAD [22,23]. The current study investigated the role of circ_0020123 and its host gene PDZD8 in LUAD. PDZD8 showed high expression levels in LUAD tissues and cells and was mainly distributed in cytoplasm. PDZD8 knockdown suppressed the proliferation and migration of LUAD cells. In addition, PDZD8 expression was found to be positively correlated with circ_0020123 expression in LUAD tissues and cells. Silencing circ_0020123 downregulated both mRNA and protein levels of PDZD8 expression, while overexpressing circ_0020123 exerted the opposite effect on PDZD8 expression. Importantly, knockdown or overexpression of circ_0020123 had no effect on the transcriptional level of PDZD8. The results indicated that circ_0020123 might function as a ceRNA to regulate PDZD8.

Subsequently, we explored the shared miRNA that circ_0020123 and PDZD8 compete for in LUAD. MiRNAs are small non-protein coding RNAs that play a critical role in cancer development [24,25]. Specifically, miRNAs can promote gene degradation by binding with 3′-UTR of mRNAs at the post transcriptional level. In the ceRNA pattern, miRNAs are sponged by circRNAs, and then the suppressive effect of miRNAs on mRNAs were reversed due to circRNA-miRNA interaction [26]. Based on bioinformatics analysis, four candidate miRNAs (miR-513a-3p, miR-590-5p, miR-548c-3p, and miR-1283) that have binding site with circ_0020123 and PDZD8 were selected for this study. Among these candidate miRNAs, miR-513a-3p was reported to sensitize LUAD cells to chemotherapy by targeting GSTP1 [19], and miR-590-5p was reported to inhibit the proliferation and invasion of NSCLS cells by downregulating GAB1 expression [27]. We identified that miR-1283 was downregulated in LUAD tissues and cells. miR-1283 expression was negatively correlated with circ_0020123 expression (or PDZD8 expression) in LUAD tissues. circ_0020123 silencing upregulated miR-1283 expression in LUAD cells. MiR-1283 overexpression downregulated PDZD8 expression at both mRNA and protein levels. Moreover, circ_0020123 served as a ceRNA to bind with miR-1283, thus promoting the expression levels of PDZD8. Similar to our study, circTTBK2 promotes the development of glioma by interacting with miR-1283 and thus increases chromodomain helicase DNA binding protein 1 (CHD1) expression [28]. In addition, circGprc5a contributes to hepatocellular carcinoma progression by binding with miR-1283 to activate the YAP1/TEAD1 signaling pathway [29]. Rescue assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation and migration. The results suggested that circ_0020123 promotes cellular behaviors in LUAD by upregulating PDZD8. In conclusion, circ_0020123 promotes the proliferation and migration of LUAD cells by serving as a ceRNA of miR-1283 to upregulate PDZD8 expression. The study reveals the promising role of circ_0020123/miR-1283/PDZD8 axis in LUAD, which may provide potential biomarkers and therapeutic approach for LUAD.

There are some limitations in the study. First, only experiments at cellular level were conducted and in vivo experiments were not designed. Xenograft tumor model in nude mice will be established in future studies to explore the influence of circ_0020123/miR-1283/PDZD8 on tumor growth and metastasis in vivo. Second, potential downstream signaling mediated by PDZD8 was not investigated. More genes involved in the circ_0020123/miR-1283/PDZD8 axis will be further explored.

Acknowledgements

Not applicable.

  1. Funding information: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

  2. Conflict of interest: Authors state no conflict of interest.

  3. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Received: 2021-08-24
Revised: 2021-12-27
Accepted: 2022-01-05
Published Online: 2022-03-17

© 2022 Wei Wei et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
  194. A rare case of endometrial polyp complicated with uterine inversion: A case report and clinical management
  195. Spontaneous rupturing of splenic artery aneurysm: Another reason for fatal syncope and shock (Case report and literature review)
  196. Fungal infection mimicking COVID-19 infection – A case report
  197. Concurrent aspergillosis and cystic pulmonary metastases in a patient with tongue squamous cell carcinoma
  198. Paraganglioma-induced inverted takotsubo-like cardiomyopathy leading to cardiogenic shock successfully treated with extracorporeal membrane oxygenation
  199. Lineage switch from lymphoma to myeloid neoplasms: First case series from a single institution
  200. Trismus during tracheal extubation as a complication of general anaesthesia – A case report
  201. Simultaneous treatment of a pubovesical fistula and lymph node metastasis secondary to multimodal treatment for prostate cancer: Case report and review of the literature
  202. Two case reports of skin vasculitis following the COVID-19 immunization
  203. Ureteroiliac fistula after oncological surgery: Case report and review of the literature
  204. Synchronous triple primary malignant tumours in the bladder, prostate, and lung harbouring TP53 and MEK1 mutations accompanied with severe cardiovascular diseases: A case report
  205. Huge mucinous cystic neoplasms with adhesion to the left colon: A case report and literature review
  206. Commentary
  207. Commentary on “Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma”
  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
  215. Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
https://doi.org/10.1515/med-2022-0434
Keywords for this article
lung adenocarcinoma; PDZD8; circ_0020123; miR-1283; ceRNA
Creative Commons
BY 4.0

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  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
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  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
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  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
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  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
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  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
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