Home LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
Article Open Access

LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis

  • Weiwei Qian , Linlin Yang , Yi Ni , Fei Yin , Lili Qin EMAIL logo and Yang Yang EMAIL logo
Published/Copyright: August 10, 2022
Download Article (PDF)
Open Medicine
From the journal Open Medicine Volume 17 Issue 1

Abstract

Long non-coding RNAs have been confirmed closely related to the metastasis and angiogenesis of breast cancer (BC). LINC01857 can promote the growth and metastasis of BC cells. The present work focused on exploring the role of LINC01857 in BC metastasis and angiogenesis and investigating the possible mechanisms. The results showed that LINC01857 and CENPQ were highly expressed in BC tissues and cells, while miR-2052 was contrarily expressed. In vitro study showed that low expression of linc01857 could inhibit the migration ability and vascularization of BC cells, and mir-2052 inhibitor partially restored the effect of si-LINC01857 on the migration ability and vascularization of BC cells. Likewise, inhibition of CENPQ can partially rescue the effects of miR-2052 inhibitor on the migration ability and vascularization of BC cells. In vivo studies showed that down-regulation of LINC01857 notably suppressed tumor growth and angiogenesis in nude mice. The miR-2052 inhibitor partially restored the effects of si-LINC01857. CENPQ suppression partially rescued the effects of the miR-2052 inhibitor. To conclude, LINC01857/miR-2052/CENPQ is the potential novel target for BC treatment.

Keywords: breast cancer; LncRNA LINC01857; MiR-2052; CENPQ; angiogenesis; metastasis

1 Introduction

Breast cancer (BC) is the most common female malignancy worldwide, with the second leading cause of cancer-related death [1,2]. According to the presence or absence of estrogen or progesterone receptors and molecular markers of human epidermal growth factor 2 (ERBB2), BC is classified into three main subtypes: ERBB2 negative (ERBB2−), ERBB2 positive (ERBB2+), and triple-negative (Triple-Neg) [3]. In recent years, the BC incidence rate has increased yearly, which is very harmful to women’s physical and mental health [4]. At present, the treatment methods of BC mainly include surgery, chemotherapy, molecular therapy, and so on [5,6]. Although the clinical research on BC treatment has made rapid progress, the postoperative recurrence rate of BC is still very high, and the prognosis of BC patients has not achieved the expected effect with less than 10% 5-year survival rate [7]. Moreover, the postoperative recurrence of BC is closely associated with angiogenesis of BC, and high-density tumor neovascularization suggests a poor prognosis [8]. Therefore, study on the regulatory mechanisms of BC angiogenesis plays an essential role in preventing and treating BC.

Tumor angiogenesis refers to the generation of new blood vessels from the existing blood vessels, which is one of the crucial characteristics of tumors [9]. Tumor cells, tumor environmental stromal cells, extracellular matrix, vascular endothelial cells, and the cellular transmitters synthesized and released by them participate in this process [10]. When tumors reach a particular size, tumor cells and stromal cells in the tumor microenvironment create stimulating factors that cause endothelial cell proliferation and migration, resulting in the formation of a new vessel for nourishment and blood supply [11]. Tumor angiogenesis not only promote tumor growth but also help tumor cells fall off, enter blood vessels, or spread to the adjacent matrix, creating conditions for tumor invasion and metastasis [12]. Researchers have found that the higher the microvessel density in tumor tissues, the greater the metastatic potential and the worse the prognosis [13]. Over the years, many outstanding achievements have been made in researching the mechanisms of tumor angiogenesis [14]. However, at present, the relevant regulatory agencies of BC angiogenesis process are not completely clear.

Long non-coding RNAs (lncRNAs) are non-coding RNAs with a length of more than 200 nt [15]. They can participate in genome regulation at pre-transcriptional level, transcription level, and post-transcriptional level through epigenetic silencing and splicing regulation. Interaction between lncRNAs and microRNAs (miRNAs), lncRNAs and mRNAs, or lncRNAs and proteins, widely participates in the regulation of individual growth and development, cell apoptosis, proliferation, and differentiation [16–18]. The imbalance of lncRNA levels is related to many cancers, including BC. For example, LINC02273 was up-regulated both within BC tissues and cells. LINC02273 knockdown inhibited BC metastasis in vitro and in vivo accomplished by modulating AGR2 [19]. HOTAIR down-regulation repressed propagation and metastasis and induced apoptosis through regulating the miR-20a-5p/HMGA2 axis [20]. MEG3 was decreased within BC tissues and cells. Up-regulation of MEG3 significantly inhibited angiogenesis in vitro and in vivo by negatively regulating AKT signaling [21]. LINC00968 was reduced in BC tissues. LINC00968 up-regulation inhibited BC cell proliferation, metastasis, and tube formation abilities by inhibiting miR-423-5p [22].

LINC01857, as a pro-cancer factor, has been confirmed related to the development of many cancers. For example, LINC01857 levels were increased within diffuse large B-cell lymphoma (DLBCL) tissues and cells. LINC01857 down-regulation inhibited the proliferation and cell cycle arrest but promoted the apoptosis of DLBCL cells [23]. LINC01857 was increased within glioma and negatively correlated with the survival rate in glioma patients. LINC01857 down-regulation repressed glioma proliferation and invasiveness in vitro and in vivo [24]. Moreover, LINC01857 levels were up-regulated within BC tissues and cells. LINC01857 knockdown inhibited the growth and metastasis of BC cells [25]. However, the detailed role and possible mechanisms of LINC01857 on BC metastasis and angiogenesis remain unclear.

The present work focused on elucidating the levels of LINC01857 in BC tissues and cells and exploring the possible mechanisms of LINC01857 in functional activities of BC cell metastasis and angiogenesis, which will imply the potential of LINC01857 to be a novel potential treatment target in treating BC.

2 Materials and methods

2.1 Collection of BC tissues

Thirty BC tissues (including nine cases of ERBB2+, six cases of ERBB2−, and 15 cases of Triple-Neg) and 30 corresponding tissues were collected from the Affiliated Hospital of Nantong University. Each participant provided written informed consent for participation.

2.2 Cell lines

MCF10A cells, BC cells (MCF-7, BT474, SKBr-3, ZR-75-30, and MDA-MB-231), and HUVEC were provided by ATCC (USA) and cultured within dulbecco's modified eagle medium (DMEM) (Thermo Fisher Scientific, USA) containing 10% FBS (Thermo Fisher Scientific, USA) at 37°C and 5% CO2 conditions.

2.3 Cell transfection

si-LINC01857 (5′-CAGUGUUCAUGAAAGCAAA-3′), si-CENPQ (5′-GGUAGAGACCACAGAGU UA-3′), negative empty vectors (si-NC) (5′-GCTTCGCGCCGTAGTCTTA-3′), NC inhibitor (5′-CAG UACUUUUGUGUAGUACAA-3′)/miR-2052 inhibitor (5′-AGUUCAAAGUUACCAGCUA-3′), and NC mimic (5′-UUUGUAC UACACAAAAGUACUG-3′)/miR-2052 mimic (5′-UGUUUUGAUAAC AGUAAUGU-3′) were obtained from Ambion (Austin, USA) and transfected into BC cells (5 × 105) maintained within the six-well plates using Lipofectamine 3000 (Invitrogen, USA). Transfection efficiency was examined by qRT-PCR after 48 h.

2.4 Angiogenesis experiment

Transfected MDA-MB-231 cells (3 × 105/well) were placed in 24-well plates, and the tumor-conditioned medium (TCM) was collected after 24 h. Next, HUVEC was inoculated into 24-well plates pre-coated with Matrigel and incubated with the above-collected medium. Then cells were fixed with 4% paraformaldehyde and imaged via a light microscope (Nikon, Japan).

2.5 Wound healing assay

Transfected cells (5 × 105/well) were placed into six-well plates at the appropriate density. After cells reached 80% confluence, a wound was scratched. Cell images at 0 and 48 h were photoed with a light microscope (Nikon, Japan) (200×).

2.6 Transwell analysis

For invasion assays, Matrigel was solved with serum-free DMEM, and uniformly covered on the Transwell chamber for 1 h at 37°C. For migration and invasion assays, BC cells from the different groups were seeded into upper chambers at appropriate density. Medium containing 10% FBS was added into lower chambers. Following a 48-h incubation period at 37°C at room temperature, migrated and invaded cells were stained with crystal violet (0.1%) and photoed with a light microscope (Nikon, Japan) (200×).

2.7 qRT-PCR analysis

TRIzol reagents (Beyotime Biotechnology, Shanghai, China) were utilized to extract total RNA from gastric cancer (GC) tissues and cells. Later, cDNA was prepared from total RNA by TaqMan one-step reverse transcription (Applied Biosystems, USA) through reverse transcription. The ABI Prism 7500 system (Applied Biosystems, USA) was used for qRT-PCR following specific protocols. We measured the relative levels of LINC01857, miR-2052, CENPQ, U6, and β-actin by 2−ΔΔCt method. U6 and β-actin served as the endogenous references. Primers used in this work are shown in Table 1.

Table 1

Primer sequences

Gene name Primer sequences
LINC01857 F:5′-CAGGACTCCATTAAGGACTC-3′
R:5′-AACATCGATGTGTCCCAGGA-3′
miR-2052 F:5′-TCGGCAGGUGUUUUGAUAAC-3′
R:5′-CAGTGCGTGTCGTGGAGT-3′
CENPQ F:5′-AAGGGCACGAGACAAAGCTAA-3′
R:5′-ACCTCACTTGCCAGAATCTGA-3′
U6 F: 5′-CTCGCTTCGGCAGCACA-3′
R:5′-AACGCTTCACGAATTTGCGT-3′
β-actin F:5′-CATGTACGTTGCTATCCAGGC-3′
R:5′-CTCCTTAATGTCACGCACGAT-3′

2.8 Western blot

Protein was isolated from BC cells and measured through the BCA kit (Beyotime Biotechnology, China). Protein was extracted using 12% SDS-PAGE and then shifted into poly(vinylidene fluoride) membranes (Millipore, USA), which were incubated using 5% skimmed milk, followed by overnight incubation with primary antibodies under 4°C. After rinsing the membranes, they were incubated for 1 h using HRP-labeled secondary antibody (1:4,000, SA00004-10, Proteintech, China) under ambient temperature. Finally, the enhanced chemiluminescence kit (ECL, Millipore, Bedford, USA) was utilized to observe protein blots, whereas ImageJ software (NIH, version 4.3) was adopted for quantification. All primary antibodies used in this study included anti-VEGF (1:2,000, 19003-1-AP, Proteintech, China), anti-CD31 (1:2,000, 11265-1-AP, Proteintech, China), anti-MMP-2 (1:2,000, 10373-2-AP, Proteintech, China), anti-MMP-9 (1:2,000, 10375-2-AP, Proteintech, China), anti-CENPQ (1:2,000, ab105742, Abcam, USA), and anti-β-actin (1:2,000, 66009-1-Ig, Proteintech, China) with β-actin being the endogenous control.

2.9 Dual-luciferase reporter assay

This study sub-cloned LINC01857 or CENPQ1 WT/MUT to the pmirGLO dual-luciferase vectors (Promega, USA) for generating pmirGLO-LINC01857 or CENPQ WT/MUT to co-transfect into cells with NC mimic or miR-2052 mimic. After co-transfected for 48 h, the relative luciferase activity was measured (Promega, USA).

2.10 Xenograft model

Balb/c nude mice (6 weeks old, 18–22 g) were provided from Animal welfare and experimental procedures and were complied with national guidelines. Briefly, 100 µL of 2 × 106 MDA-MB-231 cells from different groups were injected into the axilla and routinely monitored and sacrificed on Day 28. At the end of time, the tumors were removed, and the length and width of the tumors were manually monitored using a Vernier caliper.

2.11 H&E staining assay

Tumor tissues were fixed for 24 h with 4% paraformaldehyde and then made into 4 μm slices. Afterward, the slices were differentiated for 50 s with 1% hydrochloric acid ethanol and re-stained for 2 min with 1% eosin. Finally, the pieces were photographed to observe pathological changes using a light microscope (Nikon, Japan) (200×).

2.12 Immunohistochemistry assay

The slices from different groups were sealed for 30 min with 3% bovine serum protein at 37°C. Then slides were incubated overnight with anti-VEGF (1:500, 19003-1-AP, Proteintech, China) and anti-CD31 (1:500, 11265-1-AP, Proteintech, China). Afterward, slices were incubated with a second antibody for 60 min at room temperature and then incubated for 15 min at room temperature with 4′,6-diamidino-2-phenylindole. Fields were photographed in each slice using a Leica TCS SP5 microscope (Leica Microsystems, Wetzlar, Germany).

2.13 Statistical analysis

Data were analyzed using GraphPad Prism 5.0 and presented in the form of mean ± SD. Differences between groups were compared by one-way ANOVA and Turkey’s poc host. P < 0.05 stood for statistical significance.

  1. Ethics approval and consent to participate: The experimental protocol was established, according to the ethical guidelines of the Helsinki Declaration and was approved by the Ethics Committee of Affiliated Hospital of Nantong University. Each participant provided written informed consent for participation.

3 Results

3.1 LINC01857 levels are up-regulated in BC tissues and cells

LINC01857 levels within BC patient tissue were detected via qRT-PCR analysis. LINC01857 was highly expressed in BC tissues and tissues of the three BC subtypes (ERBB2−, ERBB2+, and Triple-Neg) were compared with normal tissues (Figure 1a), and VEGF and CD31 (Figure 1b) highly expressed in BC tissues were compared with normal tissues. LINC01857 expressions in vitro were also investigated through qRT-PCR analysis. According to Figure 1c, LINC01857 levels were up-regulated in breast cells, especially in MDA-MB-231 cells. Additionally, Figure 1d indicates that VEGF and CD31 were both significantly positively correlated with LINC01857 expression.

Figure 1 LINC01857 levels are increased in BC tissues and cells: (a) expression of LINC01857 in total tumor tissues, and the three isoforms (ERBB2−, ERBB2+, and Triple-Neg) were determined by qRT-PCR; (b) VEGF and CD31 levels in BC tissues and (c) cells were detected by qRT-PCR assay; (d) the correlation of VEGF or CD31 with LINC01857 was analyzed by correlation analysis. ** P < 0.01 and *** P < 0.001 vs normal tissues or MCF10A cells.
Figure 1

LINC01857 levels are increased in BC tissues and cells: (a) expression of LINC01857 in total tumor tissues, and the three isoforms (ERBB2−, ERBB2+, and Triple-Neg) were determined by qRT-PCR; (b) VEGF and CD31 levels in BC tissues and (c) cells were detected by qRT-PCR assay; (d) the correlation of VEGF or CD31 with LINC01857 was analyzed by correlation analysis. ** P < 0.01 and *** P < 0.001 vs normal tissues or MCF10A cells.

3.2 LINC01857 down-regulation represses metastasis of BC cells and angiogenesis of HUVEC

Si-NC and si-LINC01857 were transfected into MDA-MB-231. Transfection efficiency was evaluated by qRT-PCR as displayed in Figure 2a. It showed that LINC01857 expression was obviously decreased in MDA-MB-231 cells transfected with si-LINC01857. Then angiogenesis experiment was conducted for assessing the effects of si-LINC01857 on angiogenesis of HUVEC cells. According to Figure 2b, LINC01857 down-regulation inhibited the ability of angiogenesis of HUVEC cells, and the capillary-like structure number was significantly reduced. In addition, a western blot was conducted for determining angiogenesis-related protein levels affected by si-LINC01857. Figure 2c reveals that LINC01857 low-expression suppressed the VEGF and CD31 expressions in HUVEC cells.

Figure 2 LINC01857 down-regulation inhibits metastasis of BC cells and angiogenesis of HUVEC: (a) qRT-PCR analysis was performed to evaluate the LINC01857 expressions in MDA-MB-231 transfected with si-LINC01857; (b) angiogenesis experiment was used to evaluate the effects of si-LINC01857 on the tube formation of HUVEC cells; (c) levels of angiogenesis-related proteins, including VEGF and CD31 in TCM-treated HUVEC cells, were evaluated by western blotting assay. The effects of si-LINC01857 on MDA-MB-231 cells metastasis were detected by (d) wound healing and Transwell, (e) migration, and (f) invasion analysis. (g) The levels of metastasis-related proteins, including MMP-2 and MMP-9 in MDA-MB-231 cells transfected with si-LINC0185, were evaluated by western blotting assay. ** P < 0.01 and *** P < 0.001 vs si-NC group.
Figure 2

LINC01857 down-regulation inhibits metastasis of BC cells and angiogenesis of HUVEC: (a) qRT-PCR analysis was performed to evaluate the LINC01857 expressions in MDA-MB-231 transfected with si-LINC01857; (b) angiogenesis experiment was used to evaluate the effects of si-LINC01857 on the tube formation of HUVEC cells; (c) levels of angiogenesis-related proteins, including VEGF and CD31 in TCM-treated HUVEC cells, were evaluated by western blotting assay. The effects of si-LINC01857 on MDA-MB-231 cells metastasis were detected by (d) wound healing and Transwell, (e) migration, and (f) invasion analysis. (g) The levels of metastasis-related proteins, including MMP-2 and MMP-9 in MDA-MB-231 cells transfected with si-LINC0185, were evaluated by western blotting assay. ** P < 0.01 and *** P < 0.001 vs si-NC group.

Moreover, we carried out wound healing and Transwell analysis to assess si-LINC01857 on metastasis of MDA-MB-231 cells. The results of the wound healing assay showed that low expression of LINC01857 could reduce the rate of wound healing of MDA-MB-231 cells (Figure 2d). The results of Transwell assay showed that the number of migratory (Figure 2e) and invasive cells was significantly decreased in MDA-MB-231 cells with low expression of LINC01857 (Figure 2f). Furthermore, a western blot was carried out for determining the levels of metastasis-related proteins affected by si-LINC01857. Figure 2g displays that LINC01857 down-regulation decreased MMP-2 and MMP-9 expressions in MDA-MB-231 cells. These data indicated that LINC01857 down-regulation inhibited metastasis of BC cells and angiogenesis of HUVEC.

3.3 Regulatory relationship between LINC01857 and miR-2052 in BC

In order to ascertain the possible regulatory mechanisms of LINC01857, Bioinformatics (https://www.bioinformatics.com.cn) and RNAcentral (https://rnacentral.org/), two bioinformatics tools were utilized, and dual-luciferase reporter analysis further validated the association of LINC01857 and miR-2052 (Figure 3a and b). Additionally, miR-2052 levels were reduced within BC tissues and cells via qRT-PCR analysis (Figure 3c and d). Moreover, miR-2052 levels were up-regulated in BC cells transfected with si-LINC01857 (Figure 3e). These data indicated that LINC01857 negatively regulated miR-2052 in BC.

Figure 3 Regulatory relationship between LINC01857 and miR-2052 in BC: (a) the binding sites between LINC0185 and miR-2052; (b) the interaction between LINC0185 and miR-2052 was evaluated by a dual-luciferase reporter assay. *** P < 0.001 vs NC mimic group. MiR-2052 levels in BC (c) tissues and (d) cells were detected by the qRT-PCR assay. *** P < 0.001 vs normal tissues or MCF10A cells. (e) MiR-2052 levels in BC cells transfected with si-LINC01857 were determined by qRT-PCR assay. *** P < 0.001 vs si-NC group.
Figure 3

Regulatory relationship between LINC01857 and miR-2052 in BC: (a) the binding sites between LINC0185 and miR-2052; (b) the interaction between LINC0185 and miR-2052 was evaluated by a dual-luciferase reporter assay. *** P < 0.001 vs NC mimic group. MiR-2052 levels in BC (c) tissues and (d) cells were detected by the qRT-PCR assay. *** P < 0.001 vs normal tissues or MCF10A cells. (e) MiR-2052 levels in BC cells transfected with si-LINC01857 were determined by qRT-PCR assay. *** P < 0.001 vs si-NC group.

3.4 MiR-2052 targets CENPQ in BC

Moreover, Targetscan (https://www.targetscan.org/vert_72/) bioinformatics tools were used to explore the possible miR-2052 targets, and dual-luciferase reporter analysis verified the target relationship between miR-2052 and CENPQ (Figure 4a and b). Additionally, CENPQ levels were increased within BC tissues and cells via qRT-PCR and western blot (Figure 4c–e). Moreover, CENPQ levels were decreased in miR-2052 mimic-transfected BC cells and were increased in miR-2052 inhibitor-transfected BC cells via qRT-PCR (Figure 4f). These data indicated that CENPQ was the direct target of miR-2502 in BC.

Figure 4 MiR-2052 targets CENPQ in BC: (a) the binding sites between miR-2052 and CENPQ; (b) the interaction between miR-2052 and CENPQ was evaluated by a dual-luciferase reporter assay. *** P < 0.001 vs NC mimic group. (c) CENPQ levels in BC tissues were detected by the qRT-PCR assay. *** P < 0.001 vs normal tissues. CENPQ levels in BC cells were detected by (d) qRT-PCR and (e) western blot assays. *** P < 0.001 vs MCF10A cells. (f) CENPQ levels in BC cells transfected with miR-2052 mimic/inhibitor were determined by qRT-PCR assay. ** P < 0.01 vs NC mimic group and ### P < 0.001 vs or NC inhibitor group.
Figure 4

MiR-2052 targets CENPQ in BC: (a) the binding sites between miR-2052 and CENPQ; (b) the interaction between miR-2052 and CENPQ was evaluated by a dual-luciferase reporter assay. *** P < 0.001 vs NC mimic group. (c) CENPQ levels in BC tissues were detected by the qRT-PCR assay. *** P < 0.001 vs normal tissues. CENPQ levels in BC cells were detected by (d) qRT-PCR and (e) western blot assays. *** P < 0.001 vs MCF10A cells. (f) CENPQ levels in BC cells transfected with miR-2052 mimic/inhibitor were determined by qRT-PCR assay. ** P < 0.01 vs NC mimic group and ### P < 0.001 vs or NC inhibitor group.

3.5 MiR-2052/CENPQ mediates LINC01857 functions on angiogenesis and metastasis of BC cells

To further investigate whether LINC01857 exhibited its functional effects on BC cells by regulating the miR-2052/CENPQ axis, a series of rescue assays were performed. According to the angiogenesis experiment, miR-2052 inhibitor partially restored the effects of si-LINC01857, while si-CENPQ partially rescued the effects of the miR-2052 inhibitor on the angiogenesis abilities of BC cells (Figure 5a). Similarly, miR-2052 inhibitor increased the levels of VEGF and CD31 induced with si-LINC01857, and CENPQ down-regulation exhibited the opposite effects (Figure 5b). Based on wound healing and Transwell analysis, down-regulation of miR-2052 showed the opposite role of si-LINC01857 on BC migration and invasion, while si-CENPQ partially rescued the effects of miR-2052 inhibitor (Figure 5c–e). As expected, MMP-2 and MMP-9 protein levels were up-regulated in the miR-2052 inhibitor-transfected group relative to the si-LINC01857 group. At the same time, they were down-regulated in a si-CENPQ group compared with a miR-2052 group (Figure 5f). These data indicated that miR-2052/CENPQ mediated LINC01857 functions on angiogenesis and metastasis of BC cells.

Figure 5 MiR-2052/CENPQ mediates LINC01857 functions on angiogenesis and metastasis of BC cells: (a) angiogenesis experiment was used to evaluate the angiogenesis of BC cells after transfection; (b) western blot was used to evaluate the angiogenesis-related proteins. The metastasis of BC cells after transfection was determined by (c) wound healing and Transwell, (d) migration, and (e) invasion analysis. (f) Western blot was used to evaluate the metastasis-related proteins. ** P < 0.01, *** P < 0.001 vs si-NC group, ## P < 0.01, ### P < 0.001 vs si-LINC01857 group, $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 vs si-LINC01857 + miR-2052 inhibitor group.
Figure 5

MiR-2052/CENPQ mediates LINC01857 functions on angiogenesis and metastasis of BC cells: (a) angiogenesis experiment was used to evaluate the angiogenesis of BC cells after transfection; (b) western blot was used to evaluate the angiogenesis-related proteins. The metastasis of BC cells after transfection was determined by (c) wound healing and Transwell, (d) migration, and (e) invasion analysis. (f) Western blot was used to evaluate the metastasis-related proteins. ** P < 0.01, *** P < 0.001 vs si-NC group, ## P < 0.01, ### P < 0.001 vs si-LINC01857 group, $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 vs si-LINC01857 + miR-2052 inhibitor group.

3.6 LINC01857 down-regulation inhibits BC tumor growth in vivo

To further verify the influence of LINC01857 in BC tumor growth in vivo, transfected MDA-MB-231 cells were injected into nude mice. According to Figure 6a–c, LINC01857 down-regulation inhibited the tumor volume and weight (Figure 6d). Moreover, HE staining data suggested that the tumor cells in the si-LINC01857 group were lightly stained with less mitosis, and more tumor cells were swollen, degenerated, and necrotic (Figure 6b). Furthermore, compared to the si-NC group, LINC01857 down-regulation inhibited the VEGF and CD31 expressions (Figure 6e). Interestingly, miR-2052 inhibitor partially restored the effects of si-LINC01857, and si-CENPQ exhibited the opposite effects compared with miR-2052 inhibitor. These data indicated that LINC01857 down-regulation inhibited BC tumor growth in vivo by regulating the miR-2052/CENPQ axis.

Figure 6 LINC01857 down-regulation inhibits BC tumor growth in vivo: (a) the representative pictures of mice and tumor tissues are shown (n = 6); (b) histological changes of tumors from each group were determined by HE staining; (c) the volumes and (d) weight of tumors from each group were determined; (e) the protein expressions of VEGF and CD31 in tumors from each group were detected by immunohistochemical analysis. *** P < 0.001 vs si-NC group, ### P < 0.001 vs si-LINC01857 group, and $$$ P < 0.001 vs si-LINC01857 + miR-2052 inhibitor group.
Figure 6

LINC01857 down-regulation inhibits BC tumor growth in vivo: (a) the representative pictures of mice and tumor tissues are shown (n = 6); (b) histological changes of tumors from each group were determined by HE staining; (c) the volumes and (d) weight of tumors from each group were determined; (e) the protein expressions of VEGF and CD31 in tumors from each group were detected by immunohistochemical analysis. *** P < 0.001 vs si-NC group, ### P < 0.001 vs si-LINC01857 group, and $$$ P < 0.001 vs si-LINC01857 + miR-2052 inhibitor group.

4 Discussion

Many lncRNAs exerted a crucial role in metastasis and angiogenesis of BC. RAB11B-AS1 over-expression enhanced angiogenic factor expressions (VEGFA and ANGPTL4) [26]. Besides, PCDHB17P knockdown suppressed metastasis [27]. AC073352.1 down-regulation inhibited the metastasis and angiogenesis of BC cells [28]. These findings indicate that lncRNAs exhibit an essential impact on BC metastasis and angiogenesis. In our study, LINC01857 levels were increased within BC tissues and cells. LINC01857 down-regulation repressed the migration, invasion, and angiogenesis of BC cells. Moreover, LINC01857 down-regulation inhibited the growth of BC in vivo and suppressed the VEGF and CD31 expressions. These data were consistent with previous studies [25], suggesting that LINC01857 might act as an oncogene in BC metastasis and angiogenesis.

LncRNAs have been commonly acknowledged serving as ceRNAs through regulating miRNA expressions in cancer metastasis and angiogenesis. LINC01410 down-regulation inhibited GC metastasis and angiogenesis by negatively regulating miR-532-5p [29]. H19 silence suppressed the proliferation, migration, and angiogenesis of glioma cells by modulating miR-138 [30]. Moreover, knockdown of DANCR impaired ovarian cancer tumor growth through inhibition of tumor angiogenesis via up-regulating miR-145 [31]. In our study, we conducted online bioinformatics to retrieve miRNAs interacting with LINC01857, and miR-2052 was selected, which was further confirmed by dual-luciferase reporter analysis. It has been reported that miR-2052 levels were reduced in hepatocellular carcinoma (HCC) tissues and cells. MiR-2052 over-expression suppressed the proliferation and metastasis of HCC cells in vitro and suppressed the xenograft tumor growth in vivo [32]. Moreover, miR-2052 levels were down-regulated in oral cancer tissues [33]. Our data showed that miR-2052 levels were decreased within BC tissues and cells and negatively associated with LINC01857. Functionally, miR-2052 inhibitor partially restored the impacts of si-LINC01857 on metastasis and angiogenesis of BC both in vitro and in vivo. Based on database analysis, LINC01857 expression was positively associated with CENPQ. 3′-UTR region of CENPQ contained complementary sites to miR-2052, which suggested the potential of CENPQ as the miR-2052 target, as validated through dual-luciferase reporter analysis. Recent studies indicated that CENPQ levels were increased in HCC, and closely associated with cell division cycle-associated gene alterations [34]. Thus, rescue experiments were performed to explore the role of CENPQ on miR-2052 inhibitor on metastasis and angiogenesis of BC mediated with si-LINC01857. As expected, CENPQ suppression partially restored miR-2052 inhibitor functions in metastasis and angiogenesis of BC mediated with si-LINC01857 both in vivo and in vitro.

Taken together, LINC01857 and CENPQ levels were increased, while miR-2052 levels were decreased in BC tissues and cells. Moreover, in vitro, si-LINC01857 inhibited both metastasis and angiogenesis of BC cells, and in vivo, we found that si-linc01857 inhibited tumor growth and vascularization, possibly through regulation of miR-2052/CENPQ, providing an experimental basis that LINC01857 potentially served as the novel marker for BC treatment.

However, this experiment still has some limitations, for example, the cells studied were too single and the corresponding study of cell migration was not performed at the in vivo level. To better deeply investigate the role of LINC01857 in BC, studies from more levels are still needed.

# These authors contributed equally to this work.

tel: +86-0513-85052222

  1. Funding information: This work was supported by Science and Technology Project of Nantong Municipal Health Commission (MA2020023 and MA2021027).

  2. Author contributions: Y.Y. and L.L.Q. conceived and designed the study. W.W.Q., L.L.Y., and Y.N. performed the literature search and data extraction. W.W.Q., L.L.Y., and F.Y. drafted the manuscript. All authors read and approved the final manuscript.

  3. Conflict of interest: The authors declare that they have no conflicts of interest.

  4. Data availability statement: The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

References

[1] Voith von Voithenberg L, Crocetti E, Martos C, Dimitrova N, Giusti F, Randi G, et al. Cancer registries – guardians of breast cancer biomarker information: a systematic review. Int J Biol Markers. 2019;34(2):194–9.10.1177/1724600819836097Search in Google Scholar PubMed

[2] Azam S, Eriksson M, Sjölander A, Hellgren R, Gabrielson M, Czene K, et al. Mammographic density change and risk of breast cancer. J Natl Cancer Inst. 2020;112(4):391–9.10.1093/jnci/djz149Search in Google Scholar PubMed PubMed Central

[3] Waks AG, Winer EP. Breast cancer treatment: a review. JAMA. 2019 Jan 22;321(3):288–300.10.1001/jama.2018.19323Search in Google Scholar PubMed

[4] Fahad Ullah M. Breast cancer: current perspectives on the disease status. Adv Exp Med Biol. 2019;20301–64.10.1007/978-3-030-20301-6_4Search in Google Scholar PubMed

[5] Fisusi FA, Akala EO. Drug combinations in breast cancer therapy. Pharm Nanotechnol. 2019;7(1):3–23.10.2174/2211738507666190122111224Search in Google Scholar PubMed PubMed Central

[6] Pondé NF, Zardavas D, Piccart M. Progress in adjuvant systemic therapy for breast cancer. Nat Rev Clin Oncol. 2019;16(1):27–44.10.1038/s41571-018-0089-9Search in Google Scholar PubMed

[7] Maajani K, Jalali A, Alipour S, Khodadost M, Tohidinik HR, Yazdani K. The global and regional survival rate of women with breast cancer: a systematic review and meta-analysis. Clin Breast Cancer. 2019;19(3):165–77.10.1016/j.clbc.2019.01.006Search in Google Scholar PubMed

[8] de Heer EC, Jalving M, Harris AL. HIFs, angiogenesis, and metabolism: elusive enemies in breast cancer. J Clin Invest. 2020;130(10):5074–87.10.1172/JCI137552Search in Google Scholar PubMed PubMed Central

[9] Fei L, Huimei H, Dongmin C. Pivalopril improves anti-cancer efficiency of cDDP in breast cancer through inhibiting proliferation, angiogenesis and metastasis. Biochem Biophys Res Commun. 2020;533(4):853–60.10.1016/j.bbrc.2020.07.059Search in Google Scholar PubMed

[10] Mao Y, Keller ET, Garfield DH, Shen K, Wang J. Stromal cells in tumor microenvironment and breast cancer. Cancer Metastasis Rev. 2013;32(1–2):303–15.10.1007/s10555-012-9415-3Search in Google Scholar PubMed PubMed Central

[11] Ma X, Ning S. Cyanidin-3-glucoside attenuates the angiogenesis of breast cancer via inhibiting STAT3/VEGF pathway. Phytother Res. 2019;33(1):81–9.10.1002/ptr.6201Search in Google Scholar PubMed

[12] Niu J, Ma J, Guan X, Zhao X, Li P, Zhang M. Correlation between doppler ultrasound blood flow parameters and angiogenesis and proliferation activity in breast cancer. Med Sci Monit. 2019;25:7035–41.10.12659/MSM.914395Search in Google Scholar PubMed PubMed Central

[13] Krishnapriya S, Malipatil B, Surekha S, Sundersingh S, Sridevi V, Ananthi B, et al. Microvessel density (MVD) in locally advanced breast cancer. Asian Pac J Cancer Prev. 2019;20(5):1537–45.10.31557/APJCP.2019.20.5.1537Search in Google Scholar PubMed PubMed Central

[14] Karimzadeh P, Faghih Z, Rahmani N, Eghbali F, Razmkhah M. Quantification of angiogenic factors and their clinicopathological associations in breast cancer. Eur Cytokine Netw. 2020;31(2):68–75.10.1684/ecn.2020.0447Search in Google Scholar PubMed

[15] Shen Y, Peng X, Shen C. Identification and validation of immune-related lncRNA prognostic signature for breast cancer. Genomics. 2020;112(3):2640–6.10.1016/j.ygeno.2020.02.015Search in Google Scholar PubMed

[16] Soudyab M, Iranpour M, Ghafouri-Fard S. The role of long non-coding RNAs in breast cancer. Arch Iran Med. 2016;19(7):508–17.Search in Google Scholar

[17] Fan CN, Ma L, Liu N. Systematic analysis of lncRNA-miRNA-mRNA competing endogenous RNA network identifies four-lncRNA signature as a prognostic biomarker for breast cancer. J Transl Med. 2018;16(1):1–2.10.1186/s12967-018-1640-2Search in Google Scholar PubMed PubMed Central

[18] Li X, Li Y, Yu X, Jin F. Identification and validation of stemness-related lncRNA prognostic signature for breast cancer. J Transl Med. 2020;18(1):1.10.1186/s12967-020-02497-4Search in Google Scholar PubMed PubMed Central

[19] Xiu B, Chi Y, Liu L, Chi W, Zhang Q, Chen J, et al. LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription. Mol Cancer. 2019;18(1):1–20.10.1186/s12943-019-1115-ySearch in Google Scholar PubMed PubMed Central

[20] Zhao W, Geng D, Li S, Chen Z, Sun M. LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer. Cancer Med. 2018;7(3):842–55.10.1002/cam4.1353Search in Google Scholar PubMed PubMed Central

[21] Zhang CY, Yu MS, Li X, Zhang Z, Han CR, Yan B. Overexpression of long non-coding RNA MEG3 suppresses breast cancer cell proliferation, invasion, and angiogenesis through AKT pathway. Tumour Biol. 2017;39(6):1010428317701311.10.1177/1010428317701311Search in Google Scholar PubMed

[22] Sun X, Huang T, Zhang C, Zhang S, Wang Y, Zhang Q, et al. Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p. Cell Cycle. 2019;18(16):1908–24.10.1080/15384101.2019.1632641Search in Google Scholar PubMed PubMed Central

[23] Li Q, Li B, Lu CL, Wang JY, Gao M, Gao W. LncRNA LINC01857 promotes cell growth and diminishes apoptosis via PI3K/mTOR pathway and EMT process by regulating miR-141-3p/MAP4K4 axis in diffuse large B-cell lymphoma. Cancer Gene Ther. 2020;12(10):020–00267.10.1038/s41417-020-00267-4Search in Google Scholar PubMed

[24] Hu G, Liu N, Wang H, Wang Y, Guo Z. LncRNA LINC01857 promotes growth, migration, and invasion of glioma by modulating miR-1281/TRIM65 axis. J Cell Physiol. 2019;234(12):22009–16.10.1002/jcp.28763Search in Google Scholar PubMed

[25] Xiong Y, Gu Y, Wang F, Li L, Zhu M, Wang N, et al. LINC01857 as an oncogene regulates CREB1 activation by interacting with CREBBP in breast cancer. J Cell Physiol. 2019;234(8):14031–9.10.1002/jcp.28090Search in Google Scholar PubMed

[26] Niu Y, Bao L, Chen Y, Wang C, Luo M, Zhang B, et al. HIF2-induced long noncoding RNA RAB11B-AS1 promotes hypoxia-mediated angiogenesis and breast cancer metastasis. Cancer Res. 2020;80(5):964–75.10.1158/0008-5472.CAN-19-1532Search in Google Scholar PubMed PubMed Central

[27] Zhu L, Zhang YJ, Wang B, Yang L, Zheng YQ, Sun LD, et al. PCDHB17P/miR-145-3p/MELK/NF-κB feedback loop promotes metastasis and angiogenesis of breast cancer. Front Oncol. 2021;11:660307.10.3389/fonc.2021.660307Search in Google Scholar PubMed PubMed Central

[28] Kong X, Li J, Li Y, Duan W, Qi Q, Wang T, et al. A novel long non-coding RNA AC073352.1 promotes metastasis and angiogenesis via interacting with YBX1 in breast cancer. Cell Death Dis. 2021;12(7):021–03943.10.1038/s41419-021-03943-xSearch in Google Scholar PubMed PubMed Central

[29] Zhang JX, Chen ZH, Chen DL, Tian XP, Wang CY, Zhou ZW, et al. LINC01410-miR-532-NCF2-NF-kB feedback loop promotes gastric cancer angiogenesis and metastasis. Oncogene. 2018;37(20):2660–75.10.1038/s41388-018-0162-ySearch in Google Scholar PubMed PubMed Central

[30] Liu ZZ, Tian YF, Wu H, Ouyang SY, Kuang WL. LncRNA H19 promotes glioma angiogenesis through miR-138/HIF-1α/VEGF axis. Neoplasma. 2020;67(1):111–8.10.4149/neo_2019_190121N61Search in Google Scholar PubMed

[31] Lin X, Yang F, Qi X, Li Q, Wang D, Yi T, et al. LncRNA DANCR promotes tumor growth and angiogenesis in ovarian cancer through direct targeting of miR-145. Mol Carcinog. 2019;58(12):2286–96.10.1002/mc.23117Search in Google Scholar PubMed

[32] Zhang H, Liao Z, Liu F, Su C, Zhu H, Li Y, et al. Long noncoding RNA HULC promotes hepatocellular carcinoma progression. Aging. 2019;11(20):9111–27.10.18632/aging.102378Search in Google Scholar PubMed PubMed Central

[33] Abbas SZ, Qadir MI, Muhammad SA. Systems-level differential gene expression analysis reveals new genetic variants of oral cancer. Sci Rep. 2020;10(1):1–9.10.1038/s41598-020-71346-7Search in Google Scholar PubMed PubMed Central

[34] Tao Q, Chen S, Liu J, Zhao P, Jiang L, Tu X, et al. The roles of the cell division cycle-associated gene family in hepatocellular carcinoma. J Gastrointest Oncol. 2021;12(2):781–94.10.21037/jgo-21-110Search in Google Scholar PubMed PubMed Central

Received: 2022-01-16
Revised: 2022-05-29
Accepted: 2022-07-18
Published Online: 2022-08-10

© 2022 Weiwei Qian et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Articles in the same Issue

  1. Research Articles
  2. AMBRA1 attenuates the proliferation of uveal melanoma cells
  3. A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
  4. Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
  5. Effect of gestational diabetes mellitus on lipid profile: A systematic review and meta-analysis
  6. Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4
  7. Promising novel biomarkers and candidate small-molecule drugs for lung adenocarcinoma: Evidence from bioinformatics analysis of high-throughput data
  8. Plasmapheresis: Is it a potential alternative treatment for chronic urticaria?
  9. The biomarkers of key miRNAs and gene targets associated with extranodal NK/T-cell lymphoma
  10. Gene signature to predict prognostic survival of hepatocellular carcinoma
  11. Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
  12. Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?
  13. Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?
  14. Leptin and PCSK9 concentrations are associated with vascular endothelial cytokines in patients with stable coronary heart disease
  15. Pericentric inversion of chromosome 6 and male fertility problems
  16. Staple line reinforcement with nebulized cyanoacrylate glue in laparoscopic sleeve gastrectomy: A propensity score-matched study
  17. Retrospective analysis of crescent score in clinical prognosis of IgA nephropathy
  18. Expression of DNM3 is associated with good outcome in colorectal cancer
  19. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation
  20. CRRT influences PICCO measurements in febrile critically ill patients
  21. SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis
  22. lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells
  23. circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer
  24. Prognostic value of nicotinamide N-methyltransferase in human cancers: Evidence from a meta-analysis and database validation
  25. GPC2 deficiency inhibits cell growth and metastasis in colon adenocarcinoma
  26. A pan-cancer analysis of the oncogenic role of Holliday junction recognition protein in human tumors
  27. Radiation increases COL1A1, COL3A1, and COL1A2 expression in breast cancer
  28. Association between preventable risk factors and metabolic syndrome
  29. miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
  30. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway
  31. Quercitrin protects human bronchial epithelial cells from oxidative damage
  32. Smurf2 suppresses the metastasis of hepatocellular carcinoma via ubiquitin degradation of Smad2
  33. circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury
  34. Sonoclot’s usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study
  35. Four drug metabolism-related subgroups of pancreatic adenocarcinoma in prognosis, immune infiltration, and gene mutation
  36. Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma
  37. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway
  38. Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network
  39. Evaluation of menstrual irregularities after COVID-19 vaccination: Results of the MECOVAC survey
  40. Histopathologic findings on removed stomach after sleeve gastrectomy. Do they influence the outcome?
  41. Analysis of the expression and prognostic value of MT1-MMP, β1-integrin and YAP1 in glioma
  42. Optimal diagnosis of the skin cancer using a hybrid deep neural network and grasshopper optimization algorithm
  43. miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells
  44. Clinical value of SIRT1 as a prognostic biomarker in esophageal squamous cell carcinoma, a systematic meta-analysis
  45. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
  47. hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling
  48. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network
  49. TCD hemodynamics findings in the subacute phase of anterior circulation stroke patients treated with mechanical thrombectomy
  50. Development of a risk-stratification scoring system for predicting risk of breast cancer based on non-alcoholic fatty liver disease, non-alcoholic fatty pancreas disease, and uric acid
  51. Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway
  52. circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis
  53. Human amniotic fluid as a source of stem cells
  54. lncRNA NONRATT013819.2 promotes transforming growth factor-β1-induced myofibroblastic transition of hepatic stellate cells by miR24-3p/lox
  55. NORAD modulates miR-30c-5p-LDHA to protect lung endothelial cells damage
  56. Idiopathic pulmonary fibrosis telemedicine management during COVID-19 outbreak
  57. Risk factors for adverse drug reactions associated with clopidogrel therapy
  58. Serum zinc associated with immunity and inflammatory markers in Covid-19
  59. The relationship between night shift work and breast cancer incidence: A systematic review and meta-analysis of observational studies
  60. LncRNA expression in idiopathic achalasia: New insight and preliminary exploration into pathogenesis
  61. Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway
  62. Moscatilin suppresses the inflammation from macrophages and T cells
  63. Zoledronate promotes ECM degradation and apoptosis via Wnt/β-catenin
  64. Epithelial-mesenchymal transition-related genes in coronary artery disease
  65. The effect evaluation of traditional vaginal surgery and transvaginal mesh surgery for severe pelvic organ prolapse: 5 years follow-up
  66. Repeated partial splenic artery embolization for hypersplenism improves platelet count
  67. Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR
  68. Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis
  69. miR-455-5p enhances 5-fluorouracil sensitivity in colorectal cancer cells by targeting PIK3R1 and DEPDC1
  70. The effect of tranexamic acid on the reduction of intraoperative and postoperative blood loss and thromboembolic risk in patients with hip fracture
  71. Isocitrate dehydrogenase 1 mutation in cholangiocarcinoma impairs tumor progression by sensitizing cells to ferroptosis
  72. Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway
  73. A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer
  74. Lidocaine ameliorates chronic constriction injury-induced neuropathic pain through regulating M1/M2 microglia polarization
  75. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model
  76. miR-1273h-5p suppresses CXCL12 expression and inhibits gastric cancer cell invasion and metastasis
  77. Clinical characteristics of pneumonia patients of long course of illness infected with SARS-CoV-2
  78. circRNF20 aggravates the malignancy of retinoblastoma depending on the regulation of miR-132-3p/PAX6 axis
  79. Linezolid for resistant Gram-positive bacterial infections in children under 12 years: A meta-analysis
  80. Rack1 regulates pro-inflammatory cytokines by NF-κB in diabetic nephropathy
  81. Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients
  82. Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China
  83. Let-7i-3p inhibits the cell cycle, proliferation, invasion, and migration of colorectal cancer cells via downregulating CCND1
  84. β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
  85. Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia
  86. In vitro antimicrobial activity of ozonated oil in liposome eyedrop against multidrug-resistant bacteria
  87. Potential biomarkers for inflammatory response in acute lung injury
  88. A low serum uric acid concentration predicts a poor prognosis in adult patients with candidemia
  89. Antitumor activity of recombinant oncolytic vaccinia virus with human IL2
  90. ALKBH5 inhibits TNF-α-induced apoptosis of HUVECs through Bcl-2 pathway
  91. Risk prediction of cardiovascular disease using machine learning classifiers
  92. Value of ultrasonography parameters in diagnosing polycystic ovary syndrome
  93. Bioinformatics analysis reveals three key genes and four survival genes associated with youth-onset NSCLC
  94. Identification of autophagy-related biomarkers in patients with pulmonary arterial hypertension based on bioinformatics analysis
  95. Protective effects of glaucocalyxin A on the airway of asthmatic mice
  96. Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8
  97. Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation
  98. Effectiveness and clinical benefits of new anti-diabetic drugs: A real life experience
  99. Identification of osteoporosis based on gene biomarkers using support vector machine
  100. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis
  101. miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3
  102. Association of ST-T changes with all-cause mortality among patients with peripheral T-cell lymphomas
  103. LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma
  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
  194. A rare case of endometrial polyp complicated with uterine inversion: A case report and clinical management
  195. Spontaneous rupturing of splenic artery aneurysm: Another reason for fatal syncope and shock (Case report and literature review)
  196. Fungal infection mimicking COVID-19 infection – A case report
  197. Concurrent aspergillosis and cystic pulmonary metastases in a patient with tongue squamous cell carcinoma
  198. Paraganglioma-induced inverted takotsubo-like cardiomyopathy leading to cardiogenic shock successfully treated with extracorporeal membrane oxygenation
  199. Lineage switch from lymphoma to myeloid neoplasms: First case series from a single institution
  200. Trismus during tracheal extubation as a complication of general anaesthesia – A case report
  201. Simultaneous treatment of a pubovesical fistula and lymph node metastasis secondary to multimodal treatment for prostate cancer: Case report and review of the literature
  202. Two case reports of skin vasculitis following the COVID-19 immunization
  203. Ureteroiliac fistula after oncological surgery: Case report and review of the literature
  204. Synchronous triple primary malignant tumours in the bladder, prostate, and lung harbouring TP53 and MEK1 mutations accompanied with severe cardiovascular diseases: A case report
  205. Huge mucinous cystic neoplasms with adhesion to the left colon: A case report and literature review
  206. Commentary
  207. Commentary on “Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma”
  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
  215. Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
https://doi.org/10.1515/med-2022-0525
Keywords for this article
breast cancer; LncRNA LINC01857; MiR-2052; CENPQ; angiogenesis; metastasis
Creative Commons
BY 4.0

Articles in the same Issue

  1. Research Articles
  2. AMBRA1 attenuates the proliferation of uveal melanoma cells
  3. A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
  4. Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
  5. Effect of gestational diabetes mellitus on lipid profile: A systematic review and meta-analysis
  6. Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4
  7. Promising novel biomarkers and candidate small-molecule drugs for lung adenocarcinoma: Evidence from bioinformatics analysis of high-throughput data
  8. Plasmapheresis: Is it a potential alternative treatment for chronic urticaria?
  9. The biomarkers of key miRNAs and gene targets associated with extranodal NK/T-cell lymphoma
  10. Gene signature to predict prognostic survival of hepatocellular carcinoma
  11. Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
  12. Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?
  13. Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?
  14. Leptin and PCSK9 concentrations are associated with vascular endothelial cytokines in patients with stable coronary heart disease
  15. Pericentric inversion of chromosome 6 and male fertility problems
  16. Staple line reinforcement with nebulized cyanoacrylate glue in laparoscopic sleeve gastrectomy: A propensity score-matched study
  17. Retrospective analysis of crescent score in clinical prognosis of IgA nephropathy
  18. Expression of DNM3 is associated with good outcome in colorectal cancer
  19. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation
  20. CRRT influences PICCO measurements in febrile critically ill patients
  21. SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis
  22. lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells
  23. circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer
  24. Prognostic value of nicotinamide N-methyltransferase in human cancers: Evidence from a meta-analysis and database validation
  25. GPC2 deficiency inhibits cell growth and metastasis in colon adenocarcinoma
  26. A pan-cancer analysis of the oncogenic role of Holliday junction recognition protein in human tumors
  27. Radiation increases COL1A1, COL3A1, and COL1A2 expression in breast cancer
  28. Association between preventable risk factors and metabolic syndrome
  29. miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
  30. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway
  31. Quercitrin protects human bronchial epithelial cells from oxidative damage
  32. Smurf2 suppresses the metastasis of hepatocellular carcinoma via ubiquitin degradation of Smad2
  33. circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury
  34. Sonoclot’s usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study
  35. Four drug metabolism-related subgroups of pancreatic adenocarcinoma in prognosis, immune infiltration, and gene mutation
  36. Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma
  37. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway
  38. Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network
  39. Evaluation of menstrual irregularities after COVID-19 vaccination: Results of the MECOVAC survey
  40. Histopathologic findings on removed stomach after sleeve gastrectomy. Do they influence the outcome?
  41. Analysis of the expression and prognostic value of MT1-MMP, β1-integrin and YAP1 in glioma
  42. Optimal diagnosis of the skin cancer using a hybrid deep neural network and grasshopper optimization algorithm
  43. miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells
  44. Clinical value of SIRT1 as a prognostic biomarker in esophageal squamous cell carcinoma, a systematic meta-analysis
  45. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
  47. hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling
  48. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network
  49. TCD hemodynamics findings in the subacute phase of anterior circulation stroke patients treated with mechanical thrombectomy
  50. Development of a risk-stratification scoring system for predicting risk of breast cancer based on non-alcoholic fatty liver disease, non-alcoholic fatty pancreas disease, and uric acid
  51. Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway
  52. circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis
  53. Human amniotic fluid as a source of stem cells
  54. lncRNA NONRATT013819.2 promotes transforming growth factor-β1-induced myofibroblastic transition of hepatic stellate cells by miR24-3p/lox
  55. NORAD modulates miR-30c-5p-LDHA to protect lung endothelial cells damage
  56. Idiopathic pulmonary fibrosis telemedicine management during COVID-19 outbreak
  57. Risk factors for adverse drug reactions associated with clopidogrel therapy
  58. Serum zinc associated with immunity and inflammatory markers in Covid-19
  59. The relationship between night shift work and breast cancer incidence: A systematic review and meta-analysis of observational studies
  60. LncRNA expression in idiopathic achalasia: New insight and preliminary exploration into pathogenesis
  61. Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway
  62. Moscatilin suppresses the inflammation from macrophages and T cells
  63. Zoledronate promotes ECM degradation and apoptosis via Wnt/β-catenin
  64. Epithelial-mesenchymal transition-related genes in coronary artery disease
  65. The effect evaluation of traditional vaginal surgery and transvaginal mesh surgery for severe pelvic organ prolapse: 5 years follow-up
  66. Repeated partial splenic artery embolization for hypersplenism improves platelet count
  67. Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR
  68. Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis
  69. miR-455-5p enhances 5-fluorouracil sensitivity in colorectal cancer cells by targeting PIK3R1 and DEPDC1
  70. The effect of tranexamic acid on the reduction of intraoperative and postoperative blood loss and thromboembolic risk in patients with hip fracture
  71. Isocitrate dehydrogenase 1 mutation in cholangiocarcinoma impairs tumor progression by sensitizing cells to ferroptosis
  72. Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway
  73. A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer
  74. Lidocaine ameliorates chronic constriction injury-induced neuropathic pain through regulating M1/M2 microglia polarization
  75. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model
  76. miR-1273h-5p suppresses CXCL12 expression and inhibits gastric cancer cell invasion and metastasis
  77. Clinical characteristics of pneumonia patients of long course of illness infected with SARS-CoV-2
  78. circRNF20 aggravates the malignancy of retinoblastoma depending on the regulation of miR-132-3p/PAX6 axis
  79. Linezolid for resistant Gram-positive bacterial infections in children under 12 years: A meta-analysis
  80. Rack1 regulates pro-inflammatory cytokines by NF-κB in diabetic nephropathy
  81. Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients
  82. Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China
  83. Let-7i-3p inhibits the cell cycle, proliferation, invasion, and migration of colorectal cancer cells via downregulating CCND1
  84. β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
  85. Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia
  86. In vitro antimicrobial activity of ozonated oil in liposome eyedrop against multidrug-resistant bacteria
  87. Potential biomarkers for inflammatory response in acute lung injury
  88. A low serum uric acid concentration predicts a poor prognosis in adult patients with candidemia
  89. Antitumor activity of recombinant oncolytic vaccinia virus with human IL2
  90. ALKBH5 inhibits TNF-α-induced apoptosis of HUVECs through Bcl-2 pathway
  91. Risk prediction of cardiovascular disease using machine learning classifiers
  92. Value of ultrasonography parameters in diagnosing polycystic ovary syndrome
  93. Bioinformatics analysis reveals three key genes and four survival genes associated with youth-onset NSCLC
  94. Identification of autophagy-related biomarkers in patients with pulmonary arterial hypertension based on bioinformatics analysis
  95. Protective effects of glaucocalyxin A on the airway of asthmatic mice
  96. Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8
  97. Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation
  98. Effectiveness and clinical benefits of new anti-diabetic drugs: A real life experience
  99. Identification of osteoporosis based on gene biomarkers using support vector machine
  100. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis
  101. miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3
  102. Association of ST-T changes with all-cause mortality among patients with peripheral T-cell lymphomas
  103. LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma
  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
  194. A rare case of endometrial polyp complicated with uterine inversion: A case report and clinical management
  195. Spontaneous rupturing of splenic artery aneurysm: Another reason for fatal syncope and shock (Case report and literature review)
  196. Fungal infection mimicking COVID-19 infection – A case report
  197. Concurrent aspergillosis and cystic pulmonary metastases in a patient with tongue squamous cell carcinoma
  198. Paraganglioma-induced inverted takotsubo-like cardiomyopathy leading to cardiogenic shock successfully treated with extracorporeal membrane oxygenation
  199. Lineage switch from lymphoma to myeloid neoplasms: First case series from a single institution
  200. Trismus during tracheal extubation as a complication of general anaesthesia – A case report
  201. Simultaneous treatment of a pubovesical fistula and lymph node metastasis secondary to multimodal treatment for prostate cancer: Case report and review of the literature
  202. Two case reports of skin vasculitis following the COVID-19 immunization
  203. Ureteroiliac fistula after oncological surgery: Case report and review of the literature
  204. Synchronous triple primary malignant tumours in the bladder, prostate, and lung harbouring TP53 and MEK1 mutations accompanied with severe cardiovascular diseases: A case report
  205. Huge mucinous cystic neoplasms with adhesion to the left colon: A case report and literature review
  206. Commentary
  207. Commentary on “Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma”
  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
  215. Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 10.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/med-2022-0525/html
Scroll to top button