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Expression of DNM3 is associated with good outcome in colorectal cancer

  • Shao-ang Cheng , Xin Huang , Liang Jiang EMAIL logo , Qi-Lian Liang EMAIL logo , Xiao-Cui Hong , Hai-Xia Yang , Ke-Hui Hu , Xing-Bo Luo and Hui-Jie Zhang
Published/Copyright: January 24, 2022
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Open Medicine
From the journal Open Medicine Volume 17 Issue 1

Abstract

The aim of this study is to reveal the potential value of dynamin3 (DNM3) in colorectal cancer (CRC) evaluation of clinical diagnosis and prognosis. A total of 100 tissue samples were collected from 50 patients with stages I–IV, CRC tissues (n = 50) paired with non-cancerous adjacent colorectal tissues (n = 50). The expression levels of DNM3 were detected in 50 cases of CRC tissues and 50 cases of non-cancerous adjacent colorectal tissues by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical method (IHC) was conducted to semi-quantify the expression of DNM3 protein. Results showed that the relative expression of DNM3 mRNA in CRC tissues was 0.634-fold of that in non-cancerous adjacent colorectal tissues. The positive rate of DNM3 protein in CRC tissues (42.0%) was much lower than that in non-cancerous adjacent colorectal tissues (66.0%; P < 0.05). The expression level of DNM3 protein in CRC tissues was dependent on tumor size, degree of histological differentiation, and clinical stage (P < 0.05). The expression level of DNM3 mRNA in CRC tissues was significantly correlated with tumor size and pathology classification (P < 0.05). The research shows that detecting the expression of DNM3 helps in analyzing the tumor size, degree of histological differentiation, and clinical stage. Expression of DNM3 may be associated with good outcome in CRC.

Keywords: dynamin3; colorectal cancer; clinicopathological characteristics

1 Introduction

Globally, colorectal cancer (CRC) is the third most common malignancy and the second most common cause of death from malignancy. The WHO Cancer Research Center’s Globocan project estimated that there will be 1.8 million new cases of CRC and 880,000 deaths worldwide in 2018 [1]. The etiology of CRC is complex, and the exact molecular mechanism underlying its occurrence and development remains unclear. Tumor suppressor genes (TSGs) and their products have become the focus of research. An oncogene is a kind of gene that inhibits cell growth and has the potential to inhibit cancer. When inactivated, oncogenes may lead to malignant tumors. Studies showed that inactivation of multiple TSGs occurs during the development of CRC, and the inactivation mechanism includes loss of gene fragments, hypermethylation of gene promoters, and amplification of proto-oncogenes [2,3].

DNM3 is a TSG belonging to a highly conserved family of guanosine triphosphatase (GTP) molecules in biological evolution. It is involved in the formation of clathrin vesicles. Moreover, it is a signal transduction protein with GTP hydrolase activity. The molecule of DNM family proteins has five functional domains, namely, GTPase domain, intermediate domain, GTPase-effector domain, pleckstrin homology (PH) domain, and proline-rich domain (PRD) [4]. Among these domains, the PH domain can bind DNM to the cell membrane by binding to phosphatidylinositol lipids and interact with many different actin-associated proteins in the Src homologous-3 domain. Given the low affinity and weak specificity of the PH domain to negatively charged phosphatidylinositol lipids, many binding regions in the PRD domain can be connected to different functional regions of the cell membrane [5]. The structural features of DNM are widely involved in cellular functions, including foot process formation, plasma membrane and transmembrane vesicles, Golgi apparatus, network plate pseudopodia, phagocytosis, and cytokinesis [6,7,8,9]. Emerging evidence has shown that DNM3 is associated with tumor progression. Inokawa et al. have revealed that methylation of DNM3, which is downregulated by promoter methylation, predicts a poor prognosis for hepatocellular carcinoma (HCC) patients [10]. Numerous studies have reported that DNM3 inhibits the growth and metastasis of HCC by upregulating p53 expression or downregulating matrix metalloproteinase-2 MMP2 [10,11]. Similarly, DNM3 is found to play a tumor suppressive role in both colon and cervical cancers by regulating the activities of the MMP family [12,13]. However, our knowledge regarding the importance of DNM3 in tumor progression remains insufficient, and most knowledge has been obtained via observation. The precise molecular mechanism is not yet clearly understood.

In the present study, we analyzed the expression of DNM3 in CRC tissue samples and para-cancer tissue samples and its correlation with clinicopathological characteristics, and evaluated its potential clinical value in CRC based on previous studies, in the hope of providing new ideas for the diagnosis and/or treatment of CRC.

2 Materials and methods

2.1 Patients and tissue samples

Surgically removed specimens and para-tumorous colon tissues were gathered from 50 patients with CRC who underwent surgical treatment between January 2014 and January 2015 at the Affiliated Hospital of Guangdong Medical University, Guangdong, China. A total of 29 male and 21 female cases were included, with an age range of 32–81 years. The mean age was 58.4 ± 5.6 years. Among the cases, 37 were cases of CRC of the rectum and sigmoid colon, 11 cases were CRC of the right half colon, one case was CRC of descending colon and one case was CRC of the transverse colon. The eligibility criteria were as follows: (i) the patients had not suffered from a second primary cancer; (ii) the postoperative samples were confirmed by at least two pathologists; (iii) patients with CRC provided written informed consent; and (iv) the patients had not received any anticancer therapy prior to surgery. CRC tissues from the 50 patients and corresponding tissue adjacent to carcinoma specimens were collected through resection. 3–5 cm of the tissue adjacent to carcinoma was obtained from the intestine tissue. The pathology revealed no cancer lesions in colorectal tissue. Two samples were collected in vitro for 5 min. One sample was frozen with liquid nitrogen for 5 min and stored in a refrigerator at −80°C. The other specimen was fixed with 40 g/L buffer neutral formalin solution and embedded in conventional paraffin. The tumor size, number, presence of adhesion, lymph node metastasis, distant metastasis, and serum carcinoembryonic antigen (CEA) concentration were recorded.

2.2 Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay

Total RNA was extracted from snap-frozen paired carcinoma and para-tumorous colon tissues by TRIzol reagent (Invitrogen, Thermo Fisher Scientific, MA, USA), and cDNA solution was synthesized using M-MLV reagent kit (Promega Bio, WI, USA) according to the manufacturer’s protocol. RT-qPCR was performed with SYBR® Premix Ex Taq™ II (Takara Bio, Inc). The primers used in this study were as follows: β-actin forward, 5′-GGCGGCACCACCATGTACCCT-3′ and reverse, 5′-AGGGGCCGGACTCGTCATACT-3′; and DNM3 forward, 5′-AGTTCGCCTTGAGATTGAAGC-3′ and reverse, 5′-CGTGTGGGGAATAGACTCGTAAA-3′. The thermocycling conditions of qPCR were carried out at 95°C for 30 s, then a 2-step cycle procedure was used (at 95°C for 5 s and at 60°C for 30 s) for 40 cycles, with a final dissociation at 95°C for 15 s, 55°C for 30 s, and 95°C for 5 s. Data were quantified by the 2−ΔΔCq method.

2.3 Immunohistochemical assay (IHC)

Tissues were formalin-fixed paraffin-embedded and 4 µm-thick sections were prepared. These sections were de-paraffinized twice in xylene for 10 min and then rehydrated with a gradient of ethanol solution. The antigens were exposed to citric acid buffer in microwave for 10 min, and endogenous peroxidase activity was blocked with PBS at room temperature for 10 min. The sections were incubated with the corresponding primary antibodies (anti-DNM3, 1:150, Abcam, USA) at 4°C overnight, followed by a secondary antibody (HRP AffiniPure Goat Anti-Rabbit IgG [H + L]. No. a21020, 1:1,000, AmyJet Scientific Inc, Wuhan, China) at room temperature for 30 min. Afterwards, color was developed with diaminobenzidine reagent (Boster Biological Technology, Ltd, Wuhan, China) for 10 min and hematoxylin for 2 min both at room temperature.

The immunoreactivity of DNM3 was evaluated as follows: five fields were randomly observed under a microscopy at 400× magnification (Olympus, Tokyo, Japan) and the percentage and intensities of immune-stained cells were calculated. The score was based on the relative staining area. <10%, 10–30%, 31–60%, and >61% of staining area were specified as 0, 1, 2, and 3, respectively. According to the strength of the immune-staining cells, another four-grade score was: 0, absent; 1, weak; 2, moderate; and 3, strong. The final staining score was calculated (area score × intensity score) as negative (0–2) or positive (≥3).

2.4 Statistical analysis

All statistical analyses were performed using SPSS version 21.0 software. The values were presented as the mean value ± standard error of the mean. Student’s t-test and chi-squared test were used to evaluate the significance of differences in laterality between the two groups. P-values of <0.05 were considered statistically significant.

  1. Ethical approval: All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee at the Affiliated Hospital of Guangdong Medical University (PJ2014058KT) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The Ethics Committee at the Affiliated Hospital of Guangdong Medical University explicitly approved this study.

3 Results

The association between DNM3 expression and clinicopathological characteristics in CRC was determined. RT-PCR and IHC were used to detect the expression of DNM3 in CRC and non-cancerous adjacent colorectal tissues. The results showed that the relative expression of DNM3 mRNA in CRC was 0.634-fold of that in non-cancerous adjacent colorectal tissues (average mRNA expression: 0.634 ± 0.211 vs 1.000 ± 0.000; Table 1). The positive rate of DNM3 protein in CRC tissues (42.0%) was much lower than that in non-cancerous adjacent colorectal tissues (66.0%, P < 0.05; Figure 1 and Table 2).

Table 1

DNM3 expression in CRC and adjacent non-tumor colorectal tissues

Characteristic DNM3 mRNA 2−ΔΔCt t-value P-value
CRC tissues 0.634 ± 0.211 14.974 <0.001
Adjacent non-tumor tissues 1.000 ± 0.000

DNM3, dynamin3; CRC, colorectal cancer.

Figure 1 IHC staining of CRC and adjacent non-tumor tissues. (a) Positive expression of DNM3 in non-cancerous adjacent colorectal tissues; (b) positive expression of DNM3 in CRC tissues; (c) negative expression of DNM3 in non-cancerous adjacent colorectal tissues; (d) negative expression of DNM3 in CRC tissues. The positive area was stained with sappanwood purple.
Figure 1

IHC staining of CRC and adjacent non-tumor tissues. (a) Positive expression of DNM3 in non-cancerous adjacent colorectal tissues; (b) positive expression of DNM3 in CRC tissues; (c) negative expression of DNM3 in non-cancerous adjacent colorectal tissues; (d) negative expression of DNM3 in CRC tissues. The positive area was stained with sappanwood purple.

Table 2

Immunohistochemistry positive rates of DNM3

Rates CRC tissues Adjacent non-tumor tissues
Positive (n) 21 33
Negative (n) 29 17
Positive rate (%) 42.0 66.0
χ 2 5.722
P-value 0.015

DNM3, dynamin3; CRC, colorectal cancer.

The expression level of DNM3 mRNA in CRC tissues was independent of sex, age, clinical stage, portal vein tumor thrombus, and CEA concentration (P > 0.05) and significantly correlated with tumor size and pathology classification (P < 0.05; Table 3).

Table 3

Association between DNM3 expression and clinicopathological parameters in CRC

Variable Cases DNM3 mRNA 2−ΔΔCt t-value P-value
Age (years)
 <50 13 0.614 ± 0.148 0.489 0.627
 ≧50 37 0.621 ± 0.174
Sex
 Male 29 0.613 ± 0.094 0.755 0.454
 Female 21 0.594 ± 0.184
Tumor size (cm)
 ≦ 5 19 0.643 ± 0.117 2.342 0.024*
 > 5 31 0.590 ± 0.139
CEA (ng/mL)
 < 10 20 0.639 ± 0.088 0.075 0.941
 ≧ 10 30 0.615 ± 0.188
TNM stage
 I–II 17 0.649 ± 0.153 0.573 0.570
 III–IV 33 0.624 ± 0.172
Differentiated degree
 Well/moderately 29 0.641 ± 0.055 11.451 <0.001*
 Poorly 21 0.561 ± 0.091

*P < 0.05.

The expression level of DNM3 protein in CRC tissues was dependent on tumor size, degree of histological differentiation, and clinical stage (P < 0.05) but not on sex, age, portal vein tumor thrombus, and CEA concentration (P > 0.05; Table 4).

Table 4

Association between DNM3 expression and clinicopathological parameters in CRC (detected by immunohistochemistry)

Variable Cases + − +% χ 2 P-value
Age (years)
 <50 13 5 8 38.5 0.090 0.764
 ≧50 37 16 21 43.2
Sex
 Male 29 14 15 48.3 1.116 0.291
 Female 21 7 14 33.3
Tumor size (cm)
 ≤5 19 12 7 63.2 5.631 0.018*
 >5 31 9 22 29.0
CEA (ng/mL)
 <10 20 9 11 45.0 0.124 0.726
 ≧10 30 12 18 40.0
TNM stage
 I–II 17 12 5 70.6 8.642 0.003*
 III–IV 33 9 24 27.3
Differentiated degree
 Well/moderately 29 16 13 55.2 4.918 0.027*
 Poorly 21 5 16 23.8

*P < 0.05.

4 Discussion

No previous studies have evaluated the role of clinical diagnosis of DNM3 on CRC. In this study, RT-qPCR and immunohistochemistry were performed, and DNM3 expression was found to be higher in adjacent non-tumor colorectal tissues than in CRC tissues. Numerous studies revealed that DNM3 can be served as an indicator to judge the severity and malignancy of the tumors [10,11,12,13]. For instance, in cervical invasive squamous cell carcinoma, Lee et al. found that inhibiting the expression of the DNM2 gene can promote the overexpression MMP2 (the main structural component of the basement membrane), leading to the easy passage of tumor cells through the basement membrane of epithelium, surrounding matrix, and into blood vessels or lymphatic vessels, as well as metastasis to other sites to form new tumor lesions [13]. Therefore, the expression of DNM2 can prevent tumor invasion and lymph node metastasis, and be used as a diagnosis indicator of 17 early cervical squamous cell carcinoma risk factors. Booken et al. found that TWIST1, a transcriptional regulatory factor that is highly expressed in peripheral blood nuclear cells of patients with Sezary syndrome, can upregulate DNM3 expression, indicating that DNM3 may play a role in the occurrence of T-cell lymphoma [14]. In addition, Shen et al. [15] first reported DNM3 expression in liver cancer tissue and found that DNM3 genes present in the tumor tissues of liver cancer is hypermethylated in the promoter region, whereas the adjacent normal tissues do not present methylation pattern. Inokawa et al. found a high level of methylated DNM3 gene promoter and low expression of DNM3 gene in 48 patients with liver cancer, as well as a negative correlation between DNM3 expression in liver cancer tissues and prognosis of the patients, suggesting that DNM3 behaves as a tumor suppressor gene [10]. The expression level in liver cancer tissues is negatively correlated with the prognosis of patients with liver cancer, possibly because methylated DNM3 can promote the expression of MMP2, facilitating the expansion of tumor cells through the basement membrane of the epithelium, invasion of surrounding stroma to enter blood vessels or lymphatic vessels, and metastasis to other sites to form new tumor lesions [10]. Zhang et al. found that DNM3 is poorly expressed in cancer tissues of patients with liver cancer with venous invasion and distant metastasis, whereas upregulated DNM3 expression can inhibit the proliferation and metastasis formation of liver cancer cells [11]. Jiang et al. found that DNM3 can regulate the expression of MMP-2 and MMP-9, weaken the malignant behavior of colon cancer, and promote colon cancer invasion and migration [12]. Review articles suggested that DNM3 might be a novel candidate gene for TSGs [16]. Therefore, DNM3 is expected to be a new target for the treatment of liver cancer. The possible mechanism is to arrest the cell cycle of liver cancer cells at the G0/G1 phase by upregulating the expression of p53 protein and promote the apoptosis of liver cancer cells to achieve the anti-tumor effect.

The present study detected DNM3 in CRC tissue samples and tissues adjacent to carcinoma specimens and examined its expression and clinicopathological characteristics. The results have shown that the positive rate of DNM3 protein expression was significantly correlated with tumor size, histological differentiation degree, and TNM stage (P < 0.05) but not with gender, age, and CEA concentration (P > 0.05). High tumor volume indicated a low positive rate of DNM3 protein expression. The positive rate of DNM3 protein expression was lower in patients with poorly differentiated degree than those with well or moderately differentiated degree, suggesting that level of DNM3 was reduced by the differentiation process. The positive rate for DNM3 protein expression in TNM staging III and IV was lower than that of staging I and II. The mRNA expression levels of DNM3 were negatively associated with tumor size and degree of histological differentiation (P < 0.05). No significant association was detected between DNM3 expression and other clinicopathological parameters, including sex, age, CEA, and TNM stage (P > 0.05). Therefore, DNM3 expression at both mRNA and protein levels in CRC tissues was lower than that of non-cancerous adjacent colorectal tissues.

In summary, our data revealed that DNM3 could be a promising clinical marker for CRC patients, monitoring the expression of DNM3 may be helpful in predicting the tumor size, TNM stage, and histological differentiation degree of CRC. Expression of DNM3 may be associated with good outcome in CRC.

These Authors contributed equally to this work.

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  1. Funding information: This work was supported by a grant from the Technology Planning Project of Guangdong Province, China (No. 2014A020212291).

  2. Author contributions: Conceptualization, Shao-Ang Cheng and Xin Huang; data analysis, Xiao-Cui Hong and Hai-Xia Yang; investigation, Ke-Hui Hu and Xing-Bo Luo; methodology providing, Liang Jiang; resources, Hui-Jie Zhang; writing – original draft, Shao-Ang Cheng; writing – review and editing, Qi-Lian Liang.

  3. Conflict of interest: The authors declare that they have no conflict of interest.

  4. Data availability statement: All the data used to support the findings of this research are available from the corresponding author.

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Received: 2021-07-16
Revised: 2021-11-10
Accepted: 2021-12-09
Published Online: 2022-01-24

© 2022 Shao-ang Cheng et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  95. Protective effects of glaucocalyxin A on the airway of asthmatic mice
  96. Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8
  97. Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation
  98. Effectiveness and clinical benefits of new anti-diabetic drugs: A real life experience
  99. Identification of osteoporosis based on gene biomarkers using support vector machine
  100. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis
  101. miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3
  102. Association of ST-T changes with all-cause mortality among patients with peripheral T-cell lymphomas
  103. LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma
  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
  189. Intrauterine Bakri balloon tamponade plus cervical cerclage for the prevention and treatment of postpartum haemorrhage in late pregnancy complicated with acute aortic dissection: Case series
  190. A case of successful pembrolizumab monotherapy in a patient with advanced lung adenocarcinoma: Use of multiple biomarkers in combination for clinical practice
  191. Unusual neurological manifestations of bilateral medial medullary infarction: A case report
  192. Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene
  193. A case report of dermatomyositis with the missed diagnosis of non-small cell lung cancer and concurrence of pulmonary tuberculosis
  194. A rare case of endometrial polyp complicated with uterine inversion: A case report and clinical management
  195. Spontaneous rupturing of splenic artery aneurysm: Another reason for fatal syncope and shock (Case report and literature review)
  196. Fungal infection mimicking COVID-19 infection – A case report
  197. Concurrent aspergillosis and cystic pulmonary metastases in a patient with tongue squamous cell carcinoma
  198. Paraganglioma-induced inverted takotsubo-like cardiomyopathy leading to cardiogenic shock successfully treated with extracorporeal membrane oxygenation
  199. Lineage switch from lymphoma to myeloid neoplasms: First case series from a single institution
  200. Trismus during tracheal extubation as a complication of general anaesthesia – A case report
  201. Simultaneous treatment of a pubovesical fistula and lymph node metastasis secondary to multimodal treatment for prostate cancer: Case report and review of the literature
  202. Two case reports of skin vasculitis following the COVID-19 immunization
  203. Ureteroiliac fistula after oncological surgery: Case report and review of the literature
  204. Synchronous triple primary malignant tumours in the bladder, prostate, and lung harbouring TP53 and MEK1 mutations accompanied with severe cardiovascular diseases: A case report
  205. Huge mucinous cystic neoplasms with adhesion to the left colon: A case report and literature review
  206. Commentary
  207. Commentary on “Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma”
  208. Rapid Communication
  209. COVID-19 fear, post-traumatic stress, growth, and the role of resilience
  210. Erratum
  211. Erratum to “Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway”
  212. Erratum to “Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study”
  213. Erratum to “lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2”
  214. Retraction
  215. Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
  216. Retraction to “miR-519d downregulates LEP expression to inhibit preeclampsia development”
  217. Special Issue Computational Intelligence Methodologies Meets Recurrent Cancers - Part II
  218. Usefulness of close surveillance for rectal cancer patients after neoadjuvant chemoradiotherapy
https://doi.org/10.1515/med-2022-0420
Keywords for this article
dynamin3; colorectal cancer; clinicopathological characteristics
Creative Commons
BY 4.0

Articles in the same Issue

  1. Research Articles
  2. AMBRA1 attenuates the proliferation of uveal melanoma cells
  3. A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
  4. Differences in complications between hepatitis B-related cirrhosis and alcohol-related cirrhosis
  5. Effect of gestational diabetes mellitus on lipid profile: A systematic review and meta-analysis
  6. Long noncoding RNA NR2F1-AS1 stimulates the tumorigenic behavior of non-small cell lung cancer cells by sponging miR-363-3p to increase SOX4
  7. Promising novel biomarkers and candidate small-molecule drugs for lung adenocarcinoma: Evidence from bioinformatics analysis of high-throughput data
  8. Plasmapheresis: Is it a potential alternative treatment for chronic urticaria?
  9. The biomarkers of key miRNAs and gene targets associated with extranodal NK/T-cell lymphoma
  10. Gene signature to predict prognostic survival of hepatocellular carcinoma
  11. Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
  12. Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?
  13. Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?
  14. Leptin and PCSK9 concentrations are associated with vascular endothelial cytokines in patients with stable coronary heart disease
  15. Pericentric inversion of chromosome 6 and male fertility problems
  16. Staple line reinforcement with nebulized cyanoacrylate glue in laparoscopic sleeve gastrectomy: A propensity score-matched study
  17. Retrospective analysis of crescent score in clinical prognosis of IgA nephropathy
  18. Expression of DNM3 is associated with good outcome in colorectal cancer
  19. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation
  20. CRRT influences PICCO measurements in febrile critically ill patients
  21. SLCO4A1-AS1 mediates pancreatic cancer development via miR-4673/KIF21B axis
  22. lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells
  23. circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer
  24. Prognostic value of nicotinamide N-methyltransferase in human cancers: Evidence from a meta-analysis and database validation
  25. GPC2 deficiency inhibits cell growth and metastasis in colon adenocarcinoma
  26. A pan-cancer analysis of the oncogenic role of Holliday junction recognition protein in human tumors
  27. Radiation increases COL1A1, COL3A1, and COL1A2 expression in breast cancer
  28. Association between preventable risk factors and metabolic syndrome
  29. miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
  30. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway
  31. Quercitrin protects human bronchial epithelial cells from oxidative damage
  32. Smurf2 suppresses the metastasis of hepatocellular carcinoma via ubiquitin degradation of Smad2
  33. circRNA_0001679/miR-338-3p/DUSP16 axis aggravates acute lung injury
  34. Sonoclot’s usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study
  35. Four drug metabolism-related subgroups of pancreatic adenocarcinoma in prognosis, immune infiltration, and gene mutation
  36. Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma
  37. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway
  38. Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network
  39. Evaluation of menstrual irregularities after COVID-19 vaccination: Results of the MECOVAC survey
  40. Histopathologic findings on removed stomach after sleeve gastrectomy. Do they influence the outcome?
  41. Analysis of the expression and prognostic value of MT1-MMP, β1-integrin and YAP1 in glioma
  42. Optimal diagnosis of the skin cancer using a hybrid deep neural network and grasshopper optimization algorithm
  43. miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells
  44. Clinical value of SIRT1 as a prognostic biomarker in esophageal squamous cell carcinoma, a systematic meta-analysis
  45. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
  46. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2
  47. hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling
  48. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network
  49. TCD hemodynamics findings in the subacute phase of anterior circulation stroke patients treated with mechanical thrombectomy
  50. Development of a risk-stratification scoring system for predicting risk of breast cancer based on non-alcoholic fatty liver disease, non-alcoholic fatty pancreas disease, and uric acid
  51. Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway
  52. circ_0062491 alleviates periodontitis via the miR-142-5p/IGF1 axis
  53. Human amniotic fluid as a source of stem cells
  54. lncRNA NONRATT013819.2 promotes transforming growth factor-β1-induced myofibroblastic transition of hepatic stellate cells by miR24-3p/lox
  55. NORAD modulates miR-30c-5p-LDHA to protect lung endothelial cells damage
  56. Idiopathic pulmonary fibrosis telemedicine management during COVID-19 outbreak
  57. Risk factors for adverse drug reactions associated with clopidogrel therapy
  58. Serum zinc associated with immunity and inflammatory markers in Covid-19
  59. The relationship between night shift work and breast cancer incidence: A systematic review and meta-analysis of observational studies
  60. LncRNA expression in idiopathic achalasia: New insight and preliminary exploration into pathogenesis
  61. Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway
  62. Moscatilin suppresses the inflammation from macrophages and T cells
  63. Zoledronate promotes ECM degradation and apoptosis via Wnt/β-catenin
  64. Epithelial-mesenchymal transition-related genes in coronary artery disease
  65. The effect evaluation of traditional vaginal surgery and transvaginal mesh surgery for severe pelvic organ prolapse: 5 years follow-up
  66. Repeated partial splenic artery embolization for hypersplenism improves platelet count
  67. Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR
  68. Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis
  69. miR-455-5p enhances 5-fluorouracil sensitivity in colorectal cancer cells by targeting PIK3R1 and DEPDC1
  70. The effect of tranexamic acid on the reduction of intraoperative and postoperative blood loss and thromboembolic risk in patients with hip fracture
  71. Isocitrate dehydrogenase 1 mutation in cholangiocarcinoma impairs tumor progression by sensitizing cells to ferroptosis
  72. Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway
  73. A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer
  74. Lidocaine ameliorates chronic constriction injury-induced neuropathic pain through regulating M1/M2 microglia polarization
  75. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model
  76. miR-1273h-5p suppresses CXCL12 expression and inhibits gastric cancer cell invasion and metastasis
  77. Clinical characteristics of pneumonia patients of long course of illness infected with SARS-CoV-2
  78. circRNF20 aggravates the malignancy of retinoblastoma depending on the regulation of miR-132-3p/PAX6 axis
  79. Linezolid for resistant Gram-positive bacterial infections in children under 12 years: A meta-analysis
  80. Rack1 regulates pro-inflammatory cytokines by NF-κB in diabetic nephropathy
  81. Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients
  82. Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China
  83. Let-7i-3p inhibits the cell cycle, proliferation, invasion, and migration of colorectal cancer cells via downregulating CCND1
  84. β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
  85. Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia
  86. In vitro antimicrobial activity of ozonated oil in liposome eyedrop against multidrug-resistant bacteria
  87. Potential biomarkers for inflammatory response in acute lung injury
  88. A low serum uric acid concentration predicts a poor prognosis in adult patients with candidemia
  89. Antitumor activity of recombinant oncolytic vaccinia virus with human IL2
  90. ALKBH5 inhibits TNF-α-induced apoptosis of HUVECs through Bcl-2 pathway
  91. Risk prediction of cardiovascular disease using machine learning classifiers
  92. Value of ultrasonography parameters in diagnosing polycystic ovary syndrome
  93. Bioinformatics analysis reveals three key genes and four survival genes associated with youth-onset NSCLC
  94. Identification of autophagy-related biomarkers in patients with pulmonary arterial hypertension based on bioinformatics analysis
  95. Protective effects of glaucocalyxin A on the airway of asthmatic mice
  96. Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8
  97. Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation
  98. Effectiveness and clinical benefits of new anti-diabetic drugs: A real life experience
  99. Identification of osteoporosis based on gene biomarkers using support vector machine
  100. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis
  101. miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3
  102. Association of ST-T changes with all-cause mortality among patients with peripheral T-cell lymphomas
  103. LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma
  104. The perinatal factors that influence the excretion of fecal calprotectin in premature-born children
  105. Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study
  106. Does the use of 3D-printed cones give a chance to postpone the use of megaprostheses in patients with large bone defects in the knee joint?
  107. lncRNA HAGLR modulates myocardial ischemia–reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis
  108. Protective effect of ghrelin on intestinal I/R injury in rats
  109. In vivo knee kinematics of an innovative prosthesis design
  110. Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis
  111. lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis
  112. Correlation of cardiac troponin T and APACHE III score with all-cause in-hospital mortality in critically ill patients with acute pulmonary embolism
  113. LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis
  114. Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer
  115. SELENBP1 inhibits progression of colorectal cancer by suppressing epithelial–mesenchymal transition
  116. Visfatin is negatively associated with coronary artery lesions in subjects with impaired fasting glucose
  117. Treatment and outcomes of mechanical complications of acute myocardial infarction during the Covid-19 era: A comparison with the pre-Covid-19 period. A systematic review and meta-analysis
  118. Neonatal stroke surveillance study protocol in the United Kingdom and Republic of Ireland
  119. Oncogenic role of TWF2 in human tumors: A pan-cancer analysis
  120. Mean corpuscular hemoglobin predicts the length of hospital stay independent of severity classification in patients with acute pancreatitis
  121. Association of gallstone and polymorphisms of UGT1A1*27 and UGT1A1*28 in patients with hepatitis B virus-related liver failure
  122. TGF-β1 upregulates Sar1a expression and induces procollagen-I secretion in hypertrophic scarring fibroblasts
  123. Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
  124. NK-cell dysfunction of acute myeloid leukemia in relation to the renin–angiotensin system and neurotransmitter genes
  125. The effect of dilution with glucose and prolonged injection time on dexamethasone-induced perineal irritation – A randomized controlled trial
  126. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
  127. Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
  128. lncRNA NORAD promotes lung cancer progression by competitively binding to miR-28-3p with E2F2
  129. Noninvasive diagnosis of AIH/PBC overlap syndrome based on prediction models
  130. lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation
  131. circ-LIMK1 regulates cisplatin resistance in lung adenocarcinoma by targeting miR-512-5p/HMGA1 axis
  132. LncRNA SNHG3 promoted cell proliferation, migration, and metastasis of esophageal squamous cell carcinoma via regulating miR-151a-3p/PFN2 axis
  133. Risk perception and affective state on work exhaustion in obstetrics during the COVID-19 pandemic
  134. lncRNA-AC130710/miR-129-5p/mGluR1 axis promote migration and invasion by activating PKCα-MAPK signal pathway in melanoma
  135. SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53
  136. Xylooligosaccharides and aerobic training regulate metabolism and behavior in rats with streptozotocin-induced type 1 diabetes
  137. Serpin family A member 1 is an oncogene in glioma and its translation is enhanced by NAD(P)H quinone dehydrogenase 1 through RNA-binding activity
  138. Silencing of CPSF7 inhibits the proliferation, migration, and invasion of lung adenocarcinoma cells by blocking the AKT/mTOR signaling pathway
  139. Ultrasound-guided lumbar plexus block versus transversus abdominis plane block for analgesia in children with hip dislocation: A double-blind, randomized trial
  140. Relationship of plasma MBP and 8-oxo-dG with brain damage in preterm
  141. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma
  142. Delayed femoral vein ligation reduces operative time and blood loss during hip disarticulation in patients with extremity tumors
  143. The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
  144. Longitudinal analysis of factors related to Helicobacter pylori infection in Chinese adults
  145. HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway
  146. Meta-analysis of early-life antibiotic use and allergic rhinitis
  147. Marital status and its correlation with age, race, and gender in prognosis of tonsil squamous cell carcinomas
  148. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells
  149. Amino acid profiles in the tissue and serum of patients with liver cancer
  150. Pain in critically ill COVID-19 patients: An Italian retrospective study
  151. Immunohistochemical distribution of Bcl-2 and p53 apoptotic markers in acetamiprid-induced nephrotoxicity
  152. Estradiol pretreatment in GnRH antagonist protocol for IVF/ICSI treatment
  153. Long non-coding RNAs LINC00689 inhibits the apoptosis of human nucleus pulposus cells via miR-3127-5p/ATG7 axis-mediated autophagy
  154. The relationship between oxygen therapy, drug therapy, and COVID-19 mortality
  155. Monitoring hypertensive disorders in pregnancy to prevent preeclampsia in pregnant women of advanced maternal age: Trial mimicking with retrospective data
  156. SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway
  157. The role of Shunaoxin pills in the treatment of chronic cerebral hypoperfusion and its main pharmacodynamic components
  158. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway
  159. Associations between morphokinetic parameters of temporary-arrest embryos and the clinical prognosis in FET cycles
  160. Long noncoding RNA WT1-AS regulates trophoblast proliferation, migration, and invasion via the microRNA-186-5p/CADM2 axis
  161. The incidence of bronchiectasis in chronic obstructive pulmonary disease
  162. Integrated bioinformatics analysis shows integrin alpha 3 is a prognostic biomarker for pancreatic cancer
  163. Inhibition of miR-21 improves pulmonary vascular responses in bronchopulmonary dysplasia by targeting the DDAH1/ADMA/NO pathway
  164. Comparison of hospitalized patients with severe pneumonia caused by COVID-19 and influenza A (H7N9 and H1N1): A retrospective study from a designated hospital
  165. lncRNA ZFAS1 promotes intervertebral disc degeneration by upregulating AAK1
  166. Pathological characteristics of liver injury induced by N,N-dimethylformamide: From humans to animal models
  167. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB
  168. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis
  169. Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer
  170. MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway
  171. Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
  172. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  173. EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways
  174. Pacemakers and methylprednisolone pulse therapy in immune-related myocarditis concomitant with complete heart block
  175. miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition
  176. Review Articles
  177. Current management of cancer pain in Italy: Expert opinion paper
  178. Hearing loss and brain disorders: A review of multiple pathologies
  179. The rationale for using low-molecular weight heparin in the therapy of symptomatic COVID-19 patients
  180. Amyotrophic lateral sclerosis and delayed onset muscle soreness in light of the impaired blink and stretch reflexes – watch out for Piezo2
  181. Interleukin-35 in autoimmune dermatoses: Current concepts
  182. Recent discoveries in microbiota dysbiosis, cholangiocytic factors, and models for studying the pathogenesis of primary sclerosing cholangitis
  183. Advantages of ketamine in pediatric anesthesia
  184. Congenital adrenal hyperplasia. Role of dentist in early diagnosis
  185. Migraine management: Non-pharmacological points for patients and health care professionals
  186. Atherogenic index of plasma and coronary artery disease: A systematic review
  187. Physiological and modulatory role of thioredoxins in the cellular function
  188. Case Reports
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