Startseite Silencing of LINC00707 suppresses cell proliferation, migration, and invasion of osteosarcoma cells by modulating miR-338-3p/AHSA1 axis
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Silencing of LINC00707 suppresses cell proliferation, migration, and invasion of osteosarcoma cells by modulating miR-338-3p/AHSA1 axis

  • Xiao-rong Zhang , Jian-li Shao EMAIL logo , Heng Li und Liang Wang EMAIL logo
Veröffentlicht/Copyright: 16. Juli 2021
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Aus der Zeitschrift Open Life Sciences Band 16 Heft 1

Abstract

Osteosarcoma is the most common type of primary malignant tumor of the bone, with a high metastatic rate and poor prognosis. Therefore, it is important to further elucidate the molecular mechanisms involved in the development of osteosarcoma and explore new molecular therapeutic targets. Long intergenic nonprotein-coding RNA 707 (LINC00707) is an oncogenic gene in several cancers. In this study, we further clarified its role and regulatory mechanism in osteosarcoma. We found that LINC00707 levels are significantly higher in the osteosarcoma cell lines SW 1353, HOS, U-2 OS, MG-63, and Saos-2 compared to those in human fetal osteoblastic cell line hFOB1.19. LINC00707 silencing suppressed cell proliferation, migration, and invasion of MG-63 and Saos-2 cells. Moreover, LINC00707 can act as a competitive endogenous RNA of miR-338-3p, and miR-338-3p inhibitor and AHSA1 overexpression alleviated the effect of LINC00707 silencing. In conclusion, we demonstrated high expression of LINC00707 in osteosarcoma cell lines and that silencing LINC00707 suppresses cell proliferation, migration, and invasion by targeting the miR-338-3p/AHSA1 axis in MG-63 and Saos-2 cells. These findings suggest that LINC00707 may serve as a potential target for osteosarcoma treatment.

Keywords: LINC00707; osteosarcoma; ceRNA; miR-338-3p; AHSA1

1 Introduction

Osteosarcoma (OS) is a common malignant tumor that originates in the bone and mainly occurs in children and adolescents younger than 20 years [1]. Similar to other solid tumors, osteosarcoma has a high degree of malignancy and a poor prognosis [1]. Although surgical techniques and chemotherapy have made significant progress in the past few decades, the survival rate of OS patients has not improved much, and the 5-year survival rate is less than 70% [2]. Osteosarcoma is a disease with a complex genetic background and, so far, the pathogenesis- and tumorigenesis-driving genes have not been clarified. Currently, the treatment methods mainly include surgery, chemotherapy, and adjuvant chemotherapy [1]. Nonetheless, 30–40% of patients with osteosarcoma are at risk of lung metastasis and recurrence, and their prognosis is poor, with a 5-year survival rate less than 30% [3]. Therefore, it is crucial to explore new biomarker molecules for osteosarcoma and determine new, targeted treatment methods to improve the efficacy of osteosarcoma treatment.

Long noncoding RNAs (lncRNAs) are a type of noncoding RNAs with a length of more than 200 nucleotides [4]. They were first regarded as transcriptional “noise”; however, recent studies have shown that several lncRNAs can regulate gene expression in various biological processes. Abnormal expression of lncRNAs has been identified in various cancer tissues or cells [4,5]. Abnormally expressed lncRNAs are involved in tumorigenesis, progression, metastasis, and chemotherapy drug resistance [4,5]. It has been reported that long intergenic nonprotein-coding RNA 707 (LINC00707) plays an oncogenic role in lung adenocarcinoma, hepatocellular carcinoma, colorectal cancer, and breast cancer [6,7,8,9]. However, its role in osteosarcoma is unclear. In the present study, we focused on further analyzing the role of LINC00707 and regulatory mechanisms in osteosarcoma to provide new references for the diagnosis and treatment of osteosarcoma.

Recent studies have shown that lncRNAs are involved in several important regulatory processes such as chromosome silencing, genomic imprinting, chromatin modification, transcriptional activation, transcriptional interference, and nuclear transport [10,11,12]. Besides these, lncRNAs also affect the expression of their target genes by regulating microRNAs (miRNAs) expression [13]. miRNAs are noncoding RNAs containing about 22 nucleotides, which play a key role in posttranscriptional regulation of gene expression through binding to the 3′ untranslated region (UTR) of target mRNAs [14,15]. lncRNAs can be used as competitive endogenous RNA (ceRNA) to sponge miRNAs and thus regulate target gene expression. This regulatory mechanism is known as the ceRNA mechanism [13]. It has been reported that LINC00707 can act as a sponge of miR-206 and miR-485-5p [6,8]. However, we are far from fully understanding the mechanism of LINC00707 in cancer cells. In this study, we further investigated whether LINC00707 can act as ceRNA in osteosarcoma.

2 Materials and methods

2.1 Cell lines

The conditionally immortalized human fetal osteoblastic cell line hFOB1.19 and human osteosarcoma cell lines (SW 1353, HOS, U-2 OS, MG-63, and Saos-2) were purchased from the Institute of Cell Bank/Institutes for Biological Sciences (Shanghai, China). Cells were cultured according to our previous study [16].

2.2 Small interfering RNA transfection and cell groups

Small interfering RNA (siRNA) targeting LINC00707 (siRNA1: 5′-GGCUUUCCAUGACCCAUAAUU-3′, siRNA2: 5′-GGAAGCCACU CCUGCAUUUUU-3′, siRNA3: 5′-GCAGGAACAUCACCAUCUUUU-3′), negative control siRNA (si-NC), miR-338-3p mimic, negative control miRNA (miR-NC), miR-338-3p inhibitor, and miR-NC inhibitor were synthesized using GenePharma Co., Ltd (Shanghai, China). Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA) was used for cell transfection according to the manufacturer’s instructions. To analyze the effect of LINC00707 silencing, MG-63 or Saos-2 cells were transfected with siNC or siRNA1 and classified as the siNC group and the siRNA1 group. To analyze whether the miR-338-3p inhibitor weakens the effect of LINC00707 silencing, MG-63 or Saos-2 cells were transfected with siRNA1 plus miR-338-3p inhibitor group or siRNA1 plus miR-NC inhibitor and classified as the siRNA1 + miR-338-3p inhibitor group and the siRNA1 + miR-NC inhibitor group. After transfected at 24 h, LINC00707 expression was measured by quantitative reverse transcription PCR (qRT-PCR).

2.3 qRT-PCR

Total RNA isolation and qRT-PCR to detect LINC00707 and miR-338-3p expression as well as their primer sequences were performed as previously described [16]. To determine the LINC00707 and miR-338-3p levels, 1 μg total RNA was reverse transcribed using random primers and M-MLV reverse transcriptase (Promega, Madison, WI, USA). qPCR was performed using SYBR Green qPCR SuperMix (Invitrogen, Carlsbad, CA, USA) on ABI PRISM® 7500 Sequence Detection System (Foster City, CA, USA). The PCR reaction conditions have been described previously [16]. Gene expression was measured in triplicate and quantified using the 2−ΔΔCt method. GAPDH was used as an internal control. The primer sequences of LINC00707 were as follows: LINC00707 forward 5′-CCAACAGGGTATCAGAATTCTC-3′ and reverse 5′-TGCTGACAATAGCCATTAGG-3′.

2.4 Cell proliferation assay

Twenty-four hours after being transfected, MG-63 and Saos-2 cells were seeded at 1 × 103 cells per well in 96-well plates. The cell proliferation assay was performed on days 1–3 using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega). The proliferation rate was calculated using the following formula: Proliferation rate = (The absorbance of each time point ÷ The absorbance of 0 h − 1) × 100% (Same sample).

2.5 Transwell assay

The transwell assay was carried out as previously described [16]. Briefly, transfected cells (5 × 104 cells) in 200 µL of 0.1% fetal bovine serum (FBS) containing medium were placed in the upper chamber. The lower chamber was filled with 10% FBS medium (600 µL). For invasion, Matrigel (BD Biosciences, San Diego, CA, USA) was precoated on the bottom of the upper chamber. Twenty-four hours later, cells on the upper chamber were removed, and the cells that passed through the membrane filter were fixed and stained. Finally, five photographs at 200× magnification were randomly collected using LEICA Microscope (Tokyo, Japan) for each well. The number of migrated and invaded cells in the five fields were counted, and the average was calculated.

2.6 Western blotting analysis

Western blotting was carried out as previously described [16]. Briefly, equal amounts of total proteins (about 30 µg) were separated using 10% SDS polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). After blocking, membranes were incubated with anti-activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) antibody (at 1:1,000 dilution, Abcam, Cambridge, MA, USA) for 1 h at 37°C. After washing in TBS with 0.5% Tween 20 (TBST), the membrane was incubated with horseradish peroxidase-conjugated secondary antibody. Finally, chemiluminescent detection was performed using enhanced chemiluminescence (ECL), and signals were recorded on X-ray films.

2.7 Plasmid construction and luciferase reporter assay

The wild-type (WT) LINC00707 sequence was cloned into luciferase reporter vector psi-CHECK2 using the forward primer (5′-CCGCTCGAGAAGCAGGCAGAGGAACGTGGAAG-3′) and reverse primer (5′-ATAAGAATGCGGCCGCGGCTCAACAGTCAAAGACAGGTT-3′), and the recombinant plasmid was termed psiCHECK-LINC00707-WT. The binding site of miR-338-3p (ATGCTGG) on psiCHECK-LINC00707-WT was mutated into CGATGAA by a site-directed mutagenesis kit (Sangon Biotech, Shanghai, China), and the plasmid was termed psiCHECK-LINC00707-mut. For the luciferase reporter assay, 293 T cells were plated at 5 × 104 cells per well in 24 well plates. Next day, psiCHECK-LINC00707-WT plus miR-338-3p mimics, psiCHECK-LINC00707-WT plus miR-NC, psiCHECK-LINC00707-mut plus miR-338-3p mimics, and psiCHECK-LINC00707-mut plus miR-NC were transfected into cells using Lipofectamine 2000 (Invitrogen). Forty-eight hours after transfection, luciferase assays were performed using the dual luciferase reporter assay system (Promega).

2.8 Statistical analysis

All assays were performed in triplicate. Statistical analysis was performed using SPSS 19.0 software package (IBM Corp, Armonk, NY, USA). Significance analysis was performed using Student t-tests. P < 0.05 was considered to indicate statistically significant differences.

3 Results

3.1 LINC00707 expression is increased in osteosarcoma cells

To investigate whether LINC00707 is abnormally expressed in osteosarcoma, qRT-PCR was performed to determine LINC00707 levels in the osteoblastic cell line hFOB1.19 and human osteosarcoma cell lines. As shown in Figure 1, LINC00707 levels are significantly higher in osteosarcoma cell lines SW 1353, HOS, U-2 OS, MG-63, and Saos-2 compared to hFOB1.19. Moreover, the highest levels of LINC00707 were observed in MG-63 and Saos-2. Therefore, these two cell lines were selected for the following assays.

Figure 1 LINC00707 expression is increased in osteosarcoma cells. LINC00707 expression in the osteoblastic cell line hFOB1.19 and human osteosarcoma cell lines was detected by qRT-PCR. *P < 0.05.
Figure 1

LINC00707 expression is increased in osteosarcoma cells. LINC00707 expression in the osteoblastic cell line hFOB1.19 and human osteosarcoma cell lines was detected by qRT-PCR. *P < 0.05.

3.2 LINC00707 silencing suppress cell proliferation, migration, and invasion

To investigate the role of LINC00707 in osteosarcoma cells, three siRNA targeting LINC00707 (siRNA1, siRNA2, and siRNA3) were transfected into MG-63 and Saos-2 cells to knockdown LINC00707. As shown in Figure 2a, LINC00707 levels were significantly lower in MG-63 and Saos-2 cells transfected with siRNA1, siRNA2, or siRNA3 compared to cells transfected with si-NC. These results indicated that siRNAs targeting LINC00707 can successfully knockdown LINC00707. siRNA1, which had the highest interference efficiency, was selected for the subsequent experiments. Next, we investigated the effect of LINC00707 silencing on cell proliferation, migration, and invasion of MG-63 and Saos-2 cells. The results of the cell proliferation assay showed that the proliferation rate of the siRNA1 group of cells was lower than that of the si-NC group of cells (Figure 2b). The results of the transwell migration assay showed that the number of the migrated and invaded cells of the siRNA1 group was lower than that of the si-NC group (Figure 2c and d). These results revealed that LINC00707 silencing can suppress cell proliferation, migration, and invasion of MG-63 and Saos-2 cells.

Figure 2 LINC00707 silencing suppressed cell proliferation, migration, and invasion. (a) LINC00707 levels in MG-63 and Saos-2 cells transfected with small interfering RNA (siRNA) targeting LINC00707 (siRNA1, siRNA2, and siRNA3) and negative control siRNA (si-NC) were measured by qRT-PCR after transfected at 24 h. (b) The effect of LINC00707 silencing by siRNA1 transfection on the proliferation of MG-63 and Saos-2 cells. (c and d) The effect of LINC00707 silencing by siRNA1 transfection on migration (c) and invasion (d) of MG-63 and Saos-2 cells. Migration as detected by the transwell assay and invasion was detected by the transwell-matrigel assay. (c and d) Left includes the photographs and right presents the quantification of the results of the migrated or invaded cell numbers per field. *P < 0.05.
Figure 2

LINC00707 silencing suppressed cell proliferation, migration, and invasion. (a) LINC00707 levels in MG-63 and Saos-2 cells transfected with small interfering RNA (siRNA) targeting LINC00707 (siRNA1, siRNA2, and siRNA3) and negative control siRNA (si-NC) were measured by qRT-PCR after transfected at 24 h. (b) The effect of LINC00707 silencing by siRNA1 transfection on the proliferation of MG-63 and Saos-2 cells. (c and d) The effect of LINC00707 silencing by siRNA1 transfection on migration (c) and invasion (d) of MG-63 and Saos-2 cells. Migration as detected by the transwell assay and invasion was detected by the transwell-matrigel assay. (c and d) Left includes the photographs and right presents the quantification of the results of the migrated or invaded cell numbers per field. *P < 0.05.

In addition, the pcDNA3.1-LINC00707 (ov-LINC00707) and pcDNA3.1 (ov-NC) were transfected into MG-63 and Saos-2 cells to overexpress LINC00707. LINC00707 levels were significantly higher in MG-63 and Saos-2 cells in the ov-LINC00707 group compared to the ov-NC group (Figure S1a). Next, the cell proliferation and the migrated and invaded cells in the ov-LINC00707 group were significantly higher than that of the ov-NC group (Figure S1b–d). These results revealed that LINC00707 overexpression can promote cell proliferation, migration, and invasion of MG-63 and Saos-2 cells.

3.3 LINC00707 acts as a ceRNA of miR-338-3p

To investigate the regulatory mechanism of LINC00707, we hypothesized that miRNAs may have binding sites on the LINC00707 sequence. As shown in Figure 3a, miR-338-3p, which inhibits proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) in osteosarcoma cells [16], has two binding sites on the LINC00707 sequence. The results of the luciferase reporter assay showed that co-transfection of miR-338-3p can decrease the relative luciferase activity of psiCHECK-LINC00707-WT transfected cells and had no effect on the relative luciferase activity of psiCHECK-LINC00707-mut transfected cells (Figure 3b), indicating that miR-338-3p can bind on LINC00707. Moreover, we found that miR-338-3p levels were not different between the si-NC and siRNA1 group of cells (Figure 3c). However, the expression of AHSA1, a target of miR-338-3p, was higher in MG-63 and Saos-2 cells than that in the hFOB1.19 cell (Figure 3d). AHSA1 expression was lower in the siRNA1 group of cells than that in the si-NC group of cells (Figure 3d). These results suggested that LINC00707 may act as a ceRNA of miR-338-3p to regulate AHSA1 expression.

Figure 3 LINC00707 acts as a ceRNA of miR-338-3p. (a) A schematic diagram of miR-338-3p binding sites on LINC00707 sequence. (b) miR-338-3p cotransfection suppressed the relative luciferase activity of cells transfected with the luciferase reporter vector psi-CHECK2 containing wild-type (WT) LINC00707 sequence (psiCHECK-LINC00707-WT) compared to negative control miRNA (miR-NC). (c) miR-338-3p levels in MG-63 and Saos-2 cells transfected with small interfering RNA (siRNA) targeting LINC00707 (siRNA1) or negative control siRNA (si-NC). (d) AHSA1 protein levels in hFOB1.19, MG-63 and Saos-2 cells transfected with siRNA1 or si-NC. *P < 0.05.
Figure 3

LINC00707 acts as a ceRNA of miR-338-3p. (a) A schematic diagram of miR-338-3p binding sites on LINC00707 sequence. (b) miR-338-3p cotransfection suppressed the relative luciferase activity of cells transfected with the luciferase reporter vector psi-CHECK2 containing wild-type (WT) LINC00707 sequence (psiCHECK-LINC00707-WT) compared to negative control miRNA (miR-NC). (c) miR-338-3p levels in MG-63 and Saos-2 cells transfected with small interfering RNA (siRNA) targeting LINC00707 (siRNA1) or negative control siRNA (si-NC). (d) AHSA1 protein levels in hFOB1.19, MG-63 and Saos-2 cells transfected with siRNA1 or si-NC. *P < 0.05.

3.4 miR-338-3p inhibitor alleviated the effect of LINC00707 silencing

To verify whether LINC00707 plays its role by acting as a ceRNA of miR-338-3p, miR-338-3p inhibitor was co-transfected with siRNA1 (miR-338-3p inhibitor + siRNA1 group) to block the effect of LINC00707 silencing on miR-338-3p/AHSA1 axis. Cotransfection of miR-NC inhibitor and siRNA1 (miR-NC inhibitor + siRNA1 group) was performed as a control. miR-338-3p expression was obviously decreased in miR-338-3p inhibitor + siRNA1 group compared with that in miR-NC inhibitor + siRNA1 group (Figure 4a). AHSA1 expression was obviously increased in cells transfected with miR-338-3p inhibitor + siRNA1 compared with that in miR-NC inhibitor + siRNA1 (Figure 4b). In addition, the results of the cell proliferation assay showed that the proliferation rate of miR-338-3p inhibitor + siRNA1 group of cells was higher than that of miR-NC inhibitor + siRNA1 group of cells (Figure 4c). The results of the transwell assay showed that the number of migrated and invaded cells transfected with miR-338-3p inhibitor + siRNA1 was higher than that of cells transfected with miR-NC inhibitor + siRNA1 (Figure 4d and e). These results revealed that miR-338-3p inhibitor alleviated the effect of LINC00707 silencing on cell proliferation, migration and invasion, and AHSA1 expression of MG-63 and Saos-2 cells.

Figure 4 miR-338-3p inhibitor alleviated the effect of LINC00707 silencing. MG-63 and Saos-2 cells were cotransfected with miR-NC inhibitor plus siRNA1 or miR-338-3p inhibitor plus siRNA1. The effect of cotransfection on cell proliferation, migration, and invasion was evaluated. (a) miR-338-3p expression was measured by qRT-PCR after transfection at 24 h. (b) AHSA1 protein level was measured by western blot assay after transfection at 48 h. (c) Cell proliferation was examined by the MTS assay. (d and e) Migration was assayed by the transwell assay and invasion by the transwell-matrigel assay. The upper panel includes the photographs, and the lower panel shows the quantification and statistical analysis of the migrated or invaded cell numbers per field. *P < 0.05.
Figure 4

miR-338-3p inhibitor alleviated the effect of LINC00707 silencing. MG-63 and Saos-2 cells were cotransfected with miR-NC inhibitor plus siRNA1 or miR-338-3p inhibitor plus siRNA1. The effect of cotransfection on cell proliferation, migration, and invasion was evaluated. (a) miR-338-3p expression was measured by qRT-PCR after transfection at 24 h. (b) AHSA1 protein level was measured by western blot assay after transfection at 48 h. (c) Cell proliferation was examined by the MTS assay. (d and e) Migration was assayed by the transwell assay and invasion by the transwell-matrigel assay. The upper panel includes the photographs, and the lower panel shows the quantification and statistical analysis of the migrated or invaded cell numbers per field. *P < 0.05.

3.5 AHSA1 overexpression alleviated the effect of LINC00707 silencing

To verify the relationship between LINC00707 and AHSA1, AHSA1 expression was overexpressed in siRNA1 transfected MG-63 and Saos-2 cells (Figure 5b). The results of the cell proliferation assay showed that the proliferation rate of ov-AHSA1 + siRNA1 group of cells was higher than that of the ov-NC + siRNA1 group of cells (Figure 5c). The results of the transwell assay showed that the number of migrated and invaded cells transfected with ov-AHSA1 + siRNA1 was higher than that of cells transfected with ov-NC + siRNA1 (Figure 5d and e). These results revealed that AHSA1 overexpression alleviated the effect of LINC00707 silencing on cell proliferation, migration, and invasion of MG-63 and Saos-2 cells.

Figure 5 AHSA1 overexpression alleviated the effect of LINC00707 silenced. MG-63 and Saos-2 cells were cotransfected with ov-NC plus siRNA1 or ov-AHSA1 plus siRNA1. The effect of cotransfection on cell proliferation, migration, and invasion was evaluated. (a) AHSA1 expression was measured by western blot in MG-63 and Saos-2 cells cotransfected with miR-NC inhibitor plus siRNA1 or miR-338-3p inhibitor plus siRNA1. (b) AHSA1 expression was measured by western blot in MG-63 and Saos-2 cells cotransfected with ov-NC plus siRNA1 or ov-AHSA1 plus siRNA1. (c) Cell proliferation was examined by the MTS assay. (d and e) Migration was assayed by the transwell assay and invasion by the transwell-matrigel assay. The upper panel includes the photographs, and the lower shows the quantification and statistical analysis of the migrated or invaded cell numbers per field. *P < 0.05.
Figure 5

AHSA1 overexpression alleviated the effect of LINC00707 silenced. MG-63 and Saos-2 cells were cotransfected with ov-NC plus siRNA1 or ov-AHSA1 plus siRNA1. The effect of cotransfection on cell proliferation, migration, and invasion was evaluated. (a) AHSA1 expression was measured by western blot in MG-63 and Saos-2 cells cotransfected with miR-NC inhibitor plus siRNA1 or miR-338-3p inhibitor plus siRNA1. (b) AHSA1 expression was measured by western blot in MG-63 and Saos-2 cells cotransfected with ov-NC plus siRNA1 or ov-AHSA1 plus siRNA1. (c) Cell proliferation was examined by the MTS assay. (d and e) Migration was assayed by the transwell assay and invasion by the transwell-matrigel assay. The upper panel includes the photographs, and the lower shows the quantification and statistical analysis of the migrated or invaded cell numbers per field. *P < 0.05.

4 Discussion

Osteosarcoma is the most common type of primary malignant tumor of the bone, with a high metastasis rate and poor prognosis [1]. Therefore, it is important to further understand molecular mechanisms involved in the development of osteosarcoma and explore new molecular therapeutic targets. The abnormal expression of lncRNAs in human malignant tumors is being gradually recognized as a regulator of tumorigenesis and development [17,18]. Many lncRNAs, such as lncRNA OR3A4, lncRNA KCNQ1OT1, and LINC00514, have been demonstrated to be involved in osteosarcoma progression, drug resistance, and prognosis [19,20,21,22]. However, the roles of lncRNAs in osteosarcoma are still far from being fully understood. LINC00707 has been shown to be an oncogenic gene in several cancers [6,7,8]. However, its role and function in osteosarcoma are unclear. In the present study, we further clarified its role and regulatory mechanism in osteosarcoma. This study will provide the basis for gene-targeted therapy and may have important clinical significance.

We found that LINC00707 levels were higher in osteosarcoma cells than that in hFOB1.19 cells, suggesting that LINC00707 may be involved in osteosarcoma progression. Next, we examined the validity of this suggestion by silencing and overexpressing LINC00707 in osteosarcoma cell lines. Our results showed that LINC00707 knockdown suppressed cell proliferation, migration, and invasion of MG-63 and Saos-2 cells, whereas LINC00707 overexpression promoted cell proliferation, migration, and invasion of MG-63 and Saos-2 cells. These results indicated that LINC00707 is involved in osteosarcoma progression and is an oncogenic gene in osteosarcoma. Therefore, LINC00707 may be a therapeutic target for the osteosarcoma treatment. Our results are consistent with previous studies, which found that knockdown of LINC00707 significantly reduced cell proliferation and suppressed cell migration and invasion in lung adenocarcinoma and hepatocellular carcinoma [7,23]. All these studies suggested that abnormally expressed LINC00707 in human malignant tumors is a regulator of tumorigenesis and cancer development.

To elucidate the mechanism of LINC00707 in osteosarcoma, we examined whether it plays the function as a ceRNA. Among all the miRNAs that have binding sites on LINC00707 sequence, miR-338-3p attracted our attention. Our previous study has demonstrated that miR-338-3p can target-inhibit the expression of AHSA1 to inhibit proliferation, migration, invasion, and EMT in osteosarcoma cells [16]. Therefore, we predicted that LINC00707 is involved in osteosarcoma progression by sponging miR-338-3p. The results of the luciferase reporter assay indicated that LINC00707 can bind miR-338-3p. In addition, miR-338-3p inhibitor alleviated the effect of LINC00707 silencing in MG-63 and Saos-2 cells. These results suggest that silencing of LINC00707 suppresses cell proliferation, migration, and invasion by sponging miR-338-3p.

Next, we found that silencing LINC00707 can decrease the protein levels of AHSA1. miR-338-3p inhibitor enhanced AHSA1 expression in LINC00707-silenced MG-63 and Saos-2 cells. AHSA1 overexpression promoted cell growth, migration, and invasion in osteosarcoma cells by activating the Wnt/β-catenin signaling pathway [24]. In addition, AHSA1 overexpression alleviated the effect of LINC00707 silencing in MG-63 and Saos-2 cells. miR-338-3p inhibitor also enhanced AHSA1 expression in LINC00707-silenced MG-63 and Saos-2 cells. These results suggest that silencing of LINC00707 suppresses cell proliferation, migration, and invasion by inhibiting AHSA1 expression. Taken together, our results revealed that silencing LINC00707 suppresses cell proliferation, migration, and invasion by targeting the miR-338-3p/AHSA1 axis in MG-63 and Saos-2 cells.

Our present study has some limitations. First, we did not examine LINC00707 expression profiles in osteosarcoma tissues. Second, although we identified that LINC00707 functions by targeting miR-338-3p/AHSA1 axis, LINC00707 may have other potential targets. Further research is warranted to identify other potential targets of LINC00707. Third, we did not verify the role of LINC00707 in animals. Despite these drawbacks, our study highlights the potential role and the mechanism of LINC00707 in osteosarcoma.

In conclusion, we demonstrated that LINC00707 is highly expressed in osteosarcoma cell lines, and silencing of LINC00707 suppresses cell proliferation, migration, and invasion by targeting the miR-338-3p/AHSA1 axis in MG-63 and Saos-2 cells. These findings suggest that LINC00707 may serve as a potential target for osteosarcoma treatment.

Xiao-rong Zhang, Jian-li Shao, and Heng Li contributed equally to this paper.

  1. Funding information: The authors state no funding involved.

  2. Conflict of interest: The authors state no conflict of interest.

  3. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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[19] Yu D, Xu X, Li S, Zhang K. LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression. Aging (Albany NY). 2020;12(8):6793–807.10.18632/aging.103043Suche in Google Scholar PubMed PubMed Central

[20] Wang X, Chen K, Zhao Z. LncRNA OR3A4 regulated the growth of osteosarcoma cells by modulating the miR-1207-5p/G6PD signaling. Onco Targets Ther. 2020;13:3117–28.10.2147/OTT.S234514Suche in Google Scholar PubMed PubMed Central

[21] Shen Y, Xu J, Pan X, Zhang Y, Weng Y, Zhou D, et al. LncRNA KCNQ1OT1 sponges miR-34c-5p to promote osteosarcoma growth via ALDOA enhanced aerobic glycolysis. Cell Death Dis. 2020;11(4):278.10.1038/s41419-020-2485-1Suche in Google Scholar PubMed PubMed Central

[22] Xu S, Gong Y, Yin Y, Xing H, Zhang N. The multiple function of long noncoding RNAs in osteosarcoma progression, drug resistance and prognosis. Biomed Pharmacother. 2020;127:110141.10.1016/j.biopha.2020.110141Suche in Google Scholar PubMed

[23] Shao HJ, Li Q, Shi T, Zhang GZ, Shao F. LINC00707 promotes cell proliferation and invasion of colorectal cancer via miR-206/FMNL2 axis. Eur Rev Med Pharmacol Sci. 2019;23(9):3749–59.Suche in Google Scholar

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Received: 2020-05-18
Revised: 2021-04-23
Accepted: 2021-06-11
Published Online: 2021-07-16

© 2021 Xiao-rong Zhang et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Artikel in diesem Heft

  1. Biomedical Sciences
  2. Research progress on the mechanism of orexin in pain regulation in different brain regions
  3. Adriamycin-resistant cells are significantly less fit than adriamycin-sensitive cells in cervical cancer
  4. Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus
  5. Iris metastasis of diffuse large B-cell lymphoma misdiagnosed as primary angle-closure glaucoma: A case report and review of the literature
  6. LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
  7. Two new inflammatory markers related to the CURB-65 score for disease severity in patients with community-acquired pneumonia: The hypersensitive C-reactive protein to albumin ratio and fibrinogen to albumin ratio
  8. Circ_0091579 enhances the malignancy of hepatocellular carcinoma via miR-1287/PDK2 axis
  9. Silencing XIST mitigated lipopolysaccharide (LPS)-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and TLR4/NF-κB signaling pathway
  10. Protocatechuic acid attenuates cerebral aneurysm formation and progression by inhibiting TNF-alpha/Nrf-2/NF-kB-mediated inflammatory mechanisms in experimental rats
  11. ABCB1 polymorphism in clopidogrel-treated Montenegrin patients
  12. Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
  13. miR-338-3p inhibits cell growth, invasion, and EMT process in neuroblastoma through targeting MMP-2
  14. Verification of neuroprotective effects of alpha-lipoic acid on chronic neuropathic pain in a chronic constriction injury rat model
  15. Circ_WWC3 overexpression decelerates the progression of osteosarcoma by regulating miR-421/PDE7B axis
  16. Knockdown of TUG1 rescues cardiomyocyte hypertrophy through targeting the miR-497/MEF2C axis
  17. MiR-146b-3p protects against AR42J cell injury in cerulein-induced acute pancreatitis model through targeting Anxa2
  18. miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
  19. Diabetes and COVID-19
  20. Discovery of novel potential KIT inhibitors for the treatment of gastrointestinal stromal tumor
  21. TEAD4 is a novel independent predictor of prognosis in LGG patients with IDH mutation
  22. circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
  23. microRNA-9-5p protects liver sinusoidal endothelial cell against oxygen glucose deprivation/reperfusion injury
  24. Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis
  25. Duodenal adenocarcinoma with skin metastasis as initial manifestation: A case report
  26. Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
  27. Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
  28. Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
  29. Gene therapy in PIDs, hemoglobin, ocular, neurodegenerative, and hemophilia B disorders
  30. Downregulation of circ_0037655 impedes glioma formation and metastasis via the regulation of miR-1229-3p/ITGB8 axis
  31. Vitamin D deficiency and cardiovascular risk in type 2 diabetes population
  32. Circ_0013359 facilitates the tumorigenicity of melanoma by regulating miR-136-5p/RAB9A axis
  33. Mechanisms of circular RNA circ_0066147 on pancreatic cancer progression
  34. lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
  35. Identification of circRNA circ-CSPP1 as a potent driver of colorectal cancer by directly targeting the miR-431/LASP1 axis
  36. Hyperhomocysteinemia exacerbates ischemia-reperfusion injury-induced acute kidney injury by mediating oxidative stress, DNA damage, JNK pathway, and apoptosis
  37. Potential prognostic markers and significant lncRNA–mRNA co-expression pairs in laryngeal squamous cell carcinoma
  38. Gamma irradiation-mediated inactivation of enveloped viruses with conservation of genome integrity: Potential application for SARS-CoV-2 inactivated vaccine development
  39. ADHFE1 is a correlative factor of patient survival in cancer
  40. The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer
  41. Is there a relationship between the prevalence of autoimmune thyroid disease and diabetic kidney disease?
  42. Immunoregulatory function of Dictyophora echinovolvata spore polysaccharides in immunocompromised mice induced by cyclophosphamide
  43. T cell epitopes of SARS-CoV-2 spike protein and conserved surface protein of Plasmodium malariae share sequence homology
  44. Anti-obesity effect and mechanism of mesenchymal stem cells influence on obese mice
  45. Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis
  46. Glucocorticoids protect HEI-OC1 cells from tunicamycin-induced cell damage via inhibiting endoplasmic reticulum stress
  47. Prognostic value of the neutrophil-to-lymphocyte ratio in acute organophosphorus pesticide poisoning
  48. Gastroprotective effects of diosgenin against HCl/ethanol-induced gastric mucosal injury through suppression of NF-κβ and myeloperoxidase activities
  49. Silencing of LINC00707 suppresses cell proliferation, migration, and invasion of osteosarcoma cells by modulating miR-338-3p/AHSA1 axis
  50. Successful extracorporeal membrane oxygenation resuscitation of patient with cardiogenic shock induced by phaeochromocytoma crisis mimicking hyperthyroidism: A case report
  51. Effects of miR-185-5p on replication of hepatitis C virus
  52. Lidocaine has antitumor effect on hepatocellular carcinoma via the circ_DYNC1H1/miR-520a-3p/USP14 axis
  53. Primary localized cutaneous nodular amyloidosis presenting as lymphatic malformation: A case report
  54. Multimodal magnetic resonance imaging analysis in the characteristics of Wilson’s disease: A case report and literature review
  55. Therapeutic potential of anticoagulant therapy in association with cytokine storm inhibition in severe cases of COVID-19: A case report
  56. Neoadjuvant immunotherapy combined with chemotherapy for locally advanced squamous cell lung carcinoma: A case report and literature review
  57. Rufinamide (RUF) suppresses inflammation and maintains the integrity of the blood–brain barrier during kainic acid-induced brain damage
  58. Inhibition of ADAM10 ameliorates doxorubicin-induced cardiac remodeling by suppressing N-cadherin cleavage
  59. Invasive ductal carcinoma and small lymphocytic lymphoma/chronic lymphocytic leukemia manifesting as a collision breast tumor: A case report and literature review
  60. Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
  61. CTLA-4 promotes lymphoma progression through tumor stem cell enrichment and immunosuppression
  62. WDR74 promotes proliferation and metastasis in colorectal cancer cells through regulating the Wnt/β-catenin signaling pathway
  63. Down-regulation of IGHG1 enhances Protoporphyrin IX accumulation and inhibits hemin biosynthesis in colorectal cancer by suppressing the MEK-FECH axis
  64. Curcumin suppresses the progression of gastric cancer by regulating circ_0056618/miR-194-5p axis
  65. Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
  66. lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
  67. A two-microRNA signature predicts the progression of male thyroid cancer
  68. Isolation of microglia from retinas of chronic ocular hypertensive rats
  69. Changes of immune cells in patients with hepatocellular carcinoma treated by radiofrequency ablation and hepatectomy, a pilot study
  70. Calcineurin Aβ gene knockdown inhibits transient outward potassium current ion channel remodeling in hypertrophic ventricular myocyte
  71. Aberrant expression of PI3K/AKT signaling is involved in apoptosis resistance of hepatocellular carcinoma
  72. Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
  73. circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
  74. Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
  75. Ubiquitin-conjugating enzyme E2T promotes tumor stem cell characteristics and migration of cervical cancer cells by regulating the GRP78/FAK pathway
  76. Carriage of HLA-DRB1*11 and 1*12 alleles and risk factors in patients with breast cancer in Burkina Faso
  77. Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
  78. Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
  79. Endothelial cell-derived SSAO can increase MLC20 phosphorylation in VSMCs
  80. Downregulation of STOX1 is a novel prognostic biomarker for glioma patients
  81. miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
  82. The molecular mechanisms underlying arecoline-induced cardiac fibrosis in rats
  83. TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
  84. The influence of MTHFR genetic polymorphisms on methotrexate therapy in pediatric acute lymphoblastic leukemia
  85. Red blood cell distribution width-standard deviation but not red blood cell distribution width-coefficient of variation as a potential index for the diagnosis of iron-deficiency anemia in mid-pregnancy women
  86. Small cell neuroendocrine carcinoma expressing alpha fetoprotein in the endometrium
  87. Superoxide dismutase and the sigma1 receptor as key elements of the antioxidant system in human gastrointestinal tract cancers
  88. Molecular characterization and phylogenetic studies of Echinococcus granulosus and Taenia multiceps coenurus cysts in slaughtered sheep in Saudi Arabia
  89. ITGB5 mutation discovered in a Chinese family with blepharophimosis-ptosis-epicanthus inversus syndrome
  90. ACTB and GAPDH appear at multiple SDS-PAGE positions, thus not suitable as reference genes for determining protein loading in techniques like Western blotting
  91. Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density
  92. 3,4-Dihydroxyphenylethanol ameliorates lipopolysaccharide-induced septic cardiac injury in a murine model
  93. Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis
  94. Expression of CDK9 in endometrial cancer tissues and its effect on the proliferation of HEC-1B
  95. Novel predictor of the occurrence of DKA in T1DM patients without infection: A combination of neutrophil/lymphocyte ratio and white blood cells
  96. Investigation of molecular regulation mechanism under the pathophysiology of subarachnoid hemorrhage
  97. miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3
  98. Bioengineering and Biotechnology
  99. Green fabrication of Co and Co3O4 nanoparticles and their biomedical applications: A review
  100. Agriculture
  101. Effects of inorganic and organic selenium sources on the growth performance of broilers in China: A meta-analysis
  102. Crop-livestock integration practices, knowledge, and attitudes among smallholder farmers: Hedging against climate change-induced shocks in semi-arid Zimbabwe
  103. Food Science and Nutrition
  104. Effect of food processing on the antioxidant activity of flavones from Polygonatum odoratum (Mill.) Druce
  105. Vitamin D and iodine status was associated with the risk and complication of type 2 diabetes mellitus in China
  106. Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”
  107. Comparison between voltammetric detection methods for abalone-flavoring liquid
  108. Composition of low-molecular-weight glutenin subunits in common wheat (Triticum aestivum L.) and their effects on the rheological properties of dough
  109. Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
  110. Investigating microplastics and potentially toxic elements contamination in canned Tuna, Salmon, and Sardine fishes from Taif markets, KSA
  111. From bench to bar side: Evaluating the red wine storage lesion
  112. Establishment of an iodine model for prevention of iodine-excess-induced thyroid dysfunction in pregnant women
  113. Plant Sciences
  114. Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
  115. Comparative analysis of the SPL gene family in five Rosaceae species: Fragaria vesca, Malus domestica, Prunus persica, Rubus occidentalis, and Pyrus pyrifolia
  116. Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
  117. Investigation of bioactivities of Taxus chinensis, Taxus cuspidata, and Taxus × media by gas chromatography-mass spectrometry
  118. Morphological structures and histochemistry of roots and shoots in Myricaria laxiflora (Tamaricaceae)
  119. Transcriptome analysis of resistance mechanism to potato wart disease
  120. In silico analysis of glycosyltransferase 2 family genes in duckweed (Spirodela polyrhiza) and its role in salt stress tolerance
  121. Comparative study on growth traits and ions regulation of zoysiagrasses under varied salinity treatments
  122. Role of MS1 homolog Ntms1 gene of tobacco infertility
  123. Biological characteristics and fungicide sensitivity of Pyricularia variabilis
  124. In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
  125. Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
  126. How photoautotrophy, photomixotrophy, and ventilation affect the stomata and fluorescence emission of pistachios rootstock?
  127. Apoplastic histochemical features of plant root walls that may facilitate ion uptake and retention
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  129. The impact of sewage sludge on the fungal communities in the rhizosphere and roots of barley and on barley yield
  130. Domestication of wild animals may provide a springboard for rapid variation of coronavirus
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  132. Molecular record for the first authentication of Isaria cicadae from Vietnam
  133. Twig biomass allocation of Betula platyphylla in different habitats in Wudalianchi Volcano, northeast China
  134. Animal Sciences
  135. Supplementation of probiotics in water beneficial growth performance, carcass traits, immune function, and antioxidant capacity in broiler chickens
  136. Predators of the giant pine scale, Marchalina hellenica (Gennadius 1883; Hemiptera: Marchalinidae), out of its natural range in Turkey
  137. Honey in wound healing: An updated review
  138. NONMMUT140591.1 may serve as a ceRNA to regulate Gata5 in UT-B knockout-induced cardiac conduction block
  139. Radiotherapy for the treatment of pulmonary hydatidosis in sheep
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  141. Retraction of “Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating microRNA-34a-5p/NOTCH1 signaling pathway”
  142. Special Issue on Reuse of Agro-Industrial By-Products
  143. An effect of positional isomerism of benzoic acid derivatives on antibacterial activity against Escherichia coli
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https://doi.org/10.1515/biol-2021-0070
Schlagwörter für diesen Artikel
LINC00707; osteosarcoma; ceRNA; miR-338-3p; AHSA1
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Biomedical Sciences
  2. Research progress on the mechanism of orexin in pain regulation in different brain regions
  3. Adriamycin-resistant cells are significantly less fit than adriamycin-sensitive cells in cervical cancer
  4. Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus
  5. Iris metastasis of diffuse large B-cell lymphoma misdiagnosed as primary angle-closure glaucoma: A case report and review of the literature
  6. LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
  7. Two new inflammatory markers related to the CURB-65 score for disease severity in patients with community-acquired pneumonia: The hypersensitive C-reactive protein to albumin ratio and fibrinogen to albumin ratio
  8. Circ_0091579 enhances the malignancy of hepatocellular carcinoma via miR-1287/PDK2 axis
  9. Silencing XIST mitigated lipopolysaccharide (LPS)-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and TLR4/NF-κB signaling pathway
  10. Protocatechuic acid attenuates cerebral aneurysm formation and progression by inhibiting TNF-alpha/Nrf-2/NF-kB-mediated inflammatory mechanisms in experimental rats
  11. ABCB1 polymorphism in clopidogrel-treated Montenegrin patients
  12. Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
  13. miR-338-3p inhibits cell growth, invasion, and EMT process in neuroblastoma through targeting MMP-2
  14. Verification of neuroprotective effects of alpha-lipoic acid on chronic neuropathic pain in a chronic constriction injury rat model
  15. Circ_WWC3 overexpression decelerates the progression of osteosarcoma by regulating miR-421/PDE7B axis
  16. Knockdown of TUG1 rescues cardiomyocyte hypertrophy through targeting the miR-497/MEF2C axis
  17. MiR-146b-3p protects against AR42J cell injury in cerulein-induced acute pancreatitis model through targeting Anxa2
  18. miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
  19. Diabetes and COVID-19
  20. Discovery of novel potential KIT inhibitors for the treatment of gastrointestinal stromal tumor
  21. TEAD4 is a novel independent predictor of prognosis in LGG patients with IDH mutation
  22. circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
  23. microRNA-9-5p protects liver sinusoidal endothelial cell against oxygen glucose deprivation/reperfusion injury
  24. Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis
  25. Duodenal adenocarcinoma with skin metastasis as initial manifestation: A case report
  26. Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
  27. Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
  28. Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
  29. Gene therapy in PIDs, hemoglobin, ocular, neurodegenerative, and hemophilia B disorders
  30. Downregulation of circ_0037655 impedes glioma formation and metastasis via the regulation of miR-1229-3p/ITGB8 axis
  31. Vitamin D deficiency and cardiovascular risk in type 2 diabetes population
  32. Circ_0013359 facilitates the tumorigenicity of melanoma by regulating miR-136-5p/RAB9A axis
  33. Mechanisms of circular RNA circ_0066147 on pancreatic cancer progression
  34. lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
  35. Identification of circRNA circ-CSPP1 as a potent driver of colorectal cancer by directly targeting the miR-431/LASP1 axis
  36. Hyperhomocysteinemia exacerbates ischemia-reperfusion injury-induced acute kidney injury by mediating oxidative stress, DNA damage, JNK pathway, and apoptosis
  37. Potential prognostic markers and significant lncRNA–mRNA co-expression pairs in laryngeal squamous cell carcinoma
  38. Gamma irradiation-mediated inactivation of enveloped viruses with conservation of genome integrity: Potential application for SARS-CoV-2 inactivated vaccine development
  39. ADHFE1 is a correlative factor of patient survival in cancer
  40. The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer
  41. Is there a relationship between the prevalence of autoimmune thyroid disease and diabetic kidney disease?
  42. Immunoregulatory function of Dictyophora echinovolvata spore polysaccharides in immunocompromised mice induced by cyclophosphamide
  43. T cell epitopes of SARS-CoV-2 spike protein and conserved surface protein of Plasmodium malariae share sequence homology
  44. Anti-obesity effect and mechanism of mesenchymal stem cells influence on obese mice
  45. Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis
  46. Glucocorticoids protect HEI-OC1 cells from tunicamycin-induced cell damage via inhibiting endoplasmic reticulum stress
  47. Prognostic value of the neutrophil-to-lymphocyte ratio in acute organophosphorus pesticide poisoning
  48. Gastroprotective effects of diosgenin against HCl/ethanol-induced gastric mucosal injury through suppression of NF-κβ and myeloperoxidase activities
  49. Silencing of LINC00707 suppresses cell proliferation, migration, and invasion of osteosarcoma cells by modulating miR-338-3p/AHSA1 axis
  50. Successful extracorporeal membrane oxygenation resuscitation of patient with cardiogenic shock induced by phaeochromocytoma crisis mimicking hyperthyroidism: A case report
  51. Effects of miR-185-5p on replication of hepatitis C virus
  52. Lidocaine has antitumor effect on hepatocellular carcinoma via the circ_DYNC1H1/miR-520a-3p/USP14 axis
  53. Primary localized cutaneous nodular amyloidosis presenting as lymphatic malformation: A case report
  54. Multimodal magnetic resonance imaging analysis in the characteristics of Wilson’s disease: A case report and literature review
  55. Therapeutic potential of anticoagulant therapy in association with cytokine storm inhibition in severe cases of COVID-19: A case report
  56. Neoadjuvant immunotherapy combined with chemotherapy for locally advanced squamous cell lung carcinoma: A case report and literature review
  57. Rufinamide (RUF) suppresses inflammation and maintains the integrity of the blood–brain barrier during kainic acid-induced brain damage
  58. Inhibition of ADAM10 ameliorates doxorubicin-induced cardiac remodeling by suppressing N-cadherin cleavage
  59. Invasive ductal carcinoma and small lymphocytic lymphoma/chronic lymphocytic leukemia manifesting as a collision breast tumor: A case report and literature review
  60. Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
  61. CTLA-4 promotes lymphoma progression through tumor stem cell enrichment and immunosuppression
  62. WDR74 promotes proliferation and metastasis in colorectal cancer cells through regulating the Wnt/β-catenin signaling pathway
  63. Down-regulation of IGHG1 enhances Protoporphyrin IX accumulation and inhibits hemin biosynthesis in colorectal cancer by suppressing the MEK-FECH axis
  64. Curcumin suppresses the progression of gastric cancer by regulating circ_0056618/miR-194-5p axis
  65. Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
  66. lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
  67. A two-microRNA signature predicts the progression of male thyroid cancer
  68. Isolation of microglia from retinas of chronic ocular hypertensive rats
  69. Changes of immune cells in patients with hepatocellular carcinoma treated by radiofrequency ablation and hepatectomy, a pilot study
  70. Calcineurin Aβ gene knockdown inhibits transient outward potassium current ion channel remodeling in hypertrophic ventricular myocyte
  71. Aberrant expression of PI3K/AKT signaling is involved in apoptosis resistance of hepatocellular carcinoma
  72. Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
  73. circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
  74. Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
  75. Ubiquitin-conjugating enzyme E2T promotes tumor stem cell characteristics and migration of cervical cancer cells by regulating the GRP78/FAK pathway
  76. Carriage of HLA-DRB1*11 and 1*12 alleles and risk factors in patients with breast cancer in Burkina Faso
  77. Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
  78. Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
  79. Endothelial cell-derived SSAO can increase MLC20 phosphorylation in VSMCs
  80. Downregulation of STOX1 is a novel prognostic biomarker for glioma patients
  81. miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
  82. The molecular mechanisms underlying arecoline-induced cardiac fibrosis in rats
  83. TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
  84. The influence of MTHFR genetic polymorphisms on methotrexate therapy in pediatric acute lymphoblastic leukemia
  85. Red blood cell distribution width-standard deviation but not red blood cell distribution width-coefficient of variation as a potential index for the diagnosis of iron-deficiency anemia in mid-pregnancy women
  86. Small cell neuroendocrine carcinoma expressing alpha fetoprotein in the endometrium
  87. Superoxide dismutase and the sigma1 receptor as key elements of the antioxidant system in human gastrointestinal tract cancers
  88. Molecular characterization and phylogenetic studies of Echinococcus granulosus and Taenia multiceps coenurus cysts in slaughtered sheep in Saudi Arabia
  89. ITGB5 mutation discovered in a Chinese family with blepharophimosis-ptosis-epicanthus inversus syndrome
  90. ACTB and GAPDH appear at multiple SDS-PAGE positions, thus not suitable as reference genes for determining protein loading in techniques like Western blotting
  91. Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density
  92. 3,4-Dihydroxyphenylethanol ameliorates lipopolysaccharide-induced septic cardiac injury in a murine model
  93. Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis
  94. Expression of CDK9 in endometrial cancer tissues and its effect on the proliferation of HEC-1B
  95. Novel predictor of the occurrence of DKA in T1DM patients without infection: A combination of neutrophil/lymphocyte ratio and white blood cells
  96. Investigation of molecular regulation mechanism under the pathophysiology of subarachnoid hemorrhage
  97. miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3
  98. Bioengineering and Biotechnology
  99. Green fabrication of Co and Co3O4 nanoparticles and their biomedical applications: A review
  100. Agriculture
  101. Effects of inorganic and organic selenium sources on the growth performance of broilers in China: A meta-analysis
  102. Crop-livestock integration practices, knowledge, and attitudes among smallholder farmers: Hedging against climate change-induced shocks in semi-arid Zimbabwe
  103. Food Science and Nutrition
  104. Effect of food processing on the antioxidant activity of flavones from Polygonatum odoratum (Mill.) Druce
  105. Vitamin D and iodine status was associated with the risk and complication of type 2 diabetes mellitus in China
  106. Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”
  107. Comparison between voltammetric detection methods for abalone-flavoring liquid
  108. Composition of low-molecular-weight glutenin subunits in common wheat (Triticum aestivum L.) and their effects on the rheological properties of dough
  109. Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
  110. Investigating microplastics and potentially toxic elements contamination in canned Tuna, Salmon, and Sardine fishes from Taif markets, KSA
  111. From bench to bar side: Evaluating the red wine storage lesion
  112. Establishment of an iodine model for prevention of iodine-excess-induced thyroid dysfunction in pregnant women
  113. Plant Sciences
  114. Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
  115. Comparative analysis of the SPL gene family in five Rosaceae species: Fragaria vesca, Malus domestica, Prunus persica, Rubus occidentalis, and Pyrus pyrifolia
  116. Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
  117. Investigation of bioactivities of Taxus chinensis, Taxus cuspidata, and Taxus × media by gas chromatography-mass spectrometry
  118. Morphological structures and histochemistry of roots and shoots in Myricaria laxiflora (Tamaricaceae)
  119. Transcriptome analysis of resistance mechanism to potato wart disease
  120. In silico analysis of glycosyltransferase 2 family genes in duckweed (Spirodela polyrhiza) and its role in salt stress tolerance
  121. Comparative study on growth traits and ions regulation of zoysiagrasses under varied salinity treatments
  122. Role of MS1 homolog Ntms1 gene of tobacco infertility
  123. Biological characteristics and fungicide sensitivity of Pyricularia variabilis
  124. In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
  125. Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
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Heruntergeladen am 9.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/biol-2021-0070/html
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