Startseite Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
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Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death

  • Haiyan Shen , Fei Zhu , Jinsheng Li , Songjia Tang , Yale Zhang und Jufang Zhang EMAIL logo
Veröffentlicht/Copyright: 6. April 2020
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Open Life Sciences
Aus der Zeitschrift Open Life Sciences Band 15 Heft 1

Abstract

Background

Radiation-induced injuries (RII) mainly result from reactive oxygen species (ROS), which are harmful compounds that can damage DNA. Asiaticoside (AC), one of the main functional components extracted from Centella asiatica, has potent pharmacological effects such as anti-inflammatory and anti-oxidant activity. However, its role in RII remains unclear.

Purpose

The purpose of the current study is to investigate whether AC can mitigate RII in vitro and in vivo.

Material and Methods

Cell model of RII was successfully established by 5J/m2 radiation in vitro. For the in vivo RII model, mice were irradiated with 5 Gy to the thorax. The degree of damage to cells or mouse tissue was determined by measuring the numbers of DNA double-strand breaks (DSBs), oxidative stress, and mouse survival rates.

Results

In the in vitro assay, AC administration significantly reduced radiation-induced growth inhibition of Escherichia coli and fibroblasts, DSBs and apoptosis of fibroblasts; in the in vivo study, AC could decrease antioxidant capacity (T-AOC) of plasma and protect mice from RII, thereby improving the survival rates of mice after radiation.

Conclusions

These novel data indicate that AC is able to prevent radiation-initiated genotoxicity by mitigating DNA damage, and might serve as a safe and effective radio-protective agent.

Keywords: Asiaticoside (AC); Radiation-induced injuries (RII); Antioxidant; DNA damage; Radio-protective

1 Introduction

Radiotherapy, one of the main methods that eradicates cancer of thoracic malignant tumors such as lung cancer, esophageal cancer and lymphoma, plays an important role in controlling the pernicious development of malignant tumor cells. However, along with the injurious effect on tumor tissues, radiation therapy induces injuries in normal tissues by the production of large amount of ROS and H2O2, which finally leads to radiation pneumonia and pulmonary fibrosis [1]. Amifostine, the only radio-protective medicine approved by FDA, has effective radio-protective ability. However, it also brings some negative side effects for the patients such as vomiting, hypertension and nausea [2]. Thus, it is urgent to develop a safe and effective therapeutic radio-protective agent for clinical use.

Radiation, as a non-specific stimulation, leads to a massive increase in ROS which subsequently attacks the DNA, resulting in functional damage of cells and tissues [3, 4]. Therefore, it is necessary to mitigate ROS to protect against radiation-induced injury. AC, a triterpenoid saponin, is one of the main functional components of Centella. Previous studies have shown that AC plays a positive role in anti-inflammation, anti-oxidation and promoting wound healing [5, 6, 7]. However, understanding of AC’s protective mechanism in radiation-induced injuries has been lacking.

In our study, we found that AC had a protective function to mitigate radiation-induced injuries in Escherichia coli and fibroblasts and fibrosis in mice, which provided new ideas for the development of agents to protect against radiation-induced DNA damage, cell apoptosis, cell fibrosis and provide an experimental basis for the use of AC as a radiation protection agent.

2 Materials and methods

2.1 Cell culture and drug treatment

Cells were cultured in Dulbecco’s modified eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS). For drug treatment, sub-confluent cells were treated with 5 J/m2 radiation for 30 min followed by treatment with 400 μg/mL AC for indicated time. For the evaluation of survival rate, E.coli and fibroblasts were pretreated with 200 μg/mL AC before radiation.

2.2 MTT assay

6×104 fibroblasts were seeded into 96-well plates according to the experimental grouping. After incubation for 48 h, 20 μL MTT solutions (5 g/L) was added to the cells for 4 h. 200 μL dimethyl sulfoxide was added to the cells after removing the supernatant and were vibrated for 10 min at low speed to make the crystals dissolve. Absorbance at the wavelength of 570 nm was read by microplate reader.

2.3 Animals

C57BL/6J mice (8-10 weeks old, male), which have been shown to be radiation sensitive, were housed five animals per cage under controlled conditions and received standard laboratory diet through the course of the experiment.

Ethical approval: The research related to animals use has been complied with all the relevant national regulations and institutional policies for the care and use of animals. Experiments were performed under protocols approved by the Committee on Animal Resources.

2.4 Irradiation treatment

The mice were randomly divided into four groups: Control, AC treatment, Radiation treatment, and AC plus Radiation treatment groups. For the irradiation, animals were restrained in plastic jigs and placed so that the thoraxes were located in the center of the field defined by the slit lead collimator and irradiated with 5 Gy to the thorax only. Treatment dose is based on the well-established threshold dose for the induction of fibrosis in the C57BL/6J strain, as previously reported using an identical exposure method. Animals were assigned to each treatment group for each time point examined. For AC treatment, AC-irradiated (5 Gy) mice received similar handling followed by administration of 25 mg/kg AC for 2 weeks on day 10 after radiation. After that, lung tissue of all mice was harvested and were used to detect histologic changes and protein/ mRNA levels of TGF-β1.

2.5 RT-PCR

RNA was extracted from frozen tissues with Trizol Reagent (Invitrogen) according to the manufacturer’s instructions. Two micrograms of RNA were reverse transcribed according to the manufacturer’s specifications. Multiplex PCR was done according to the previous description. Complementary DNA was made from RNA with a PrimeScript™ RT reagent kit, followed by quantitative PCR analysis according to the SYBR® Premix Ex Taq™ manufacturer’s instructions. The reaction program was set as follows: 37°C for 15 min, 95°C for 30 s for the initial template denaturation and 40 cycles of denaturation at 95°C for 5 s, followed by annealing and extension at 60°C for 30 s.

2.6 Comet assay

The comet assay was performed as previously described [8]. Briefly, gels were lysed overnight at 4°C. After lysis, gels were transferred to an electrophoresis chamber filled with alkaline unwinding buffer for 40 min at 4°C. Electrophoresis was conducted with the same buffer at 4°C for 30 min 1 V/cm and 300 mA. Neutralization solution was used to neutralize ethylene-bromide for 1min and then rinsed with deionized water to remove the excess dye. The number of comet tail cells was observed under the fluorescence microscope.

2.7 T-AOC assay

Total antioxidant capacity (T-AOC) in the serum of the mice were detected by a biochemical method following the instructions provided with the reagent kits [9] (T-AOC, A015; malondialdehyde, A003) purchased from Nanjing Jiancheng Bioengineering Institute of China.

2.8 Statistical analysis

Two-tailed Student’s t-tests for statistical analyses were performed with the data from three independent biological replicates using GraphPad Prism 5 to compare parameters between groups. Statistical significance was determined as p<0.05.

3 Results

3.1 AC increases cell viability of radiated or H2O2-treated Escherichia coli (E.coli)

It is confirmed that Escherichia coli lipopolysaccharide (LPS) can induce lung injury and release inflammatory factor NO [10]. In our study, 400 μg/mL AC inhibited proliferation and diminished the viability of E.coli, but there was no effect on the proliferation of E.coli when the concentration of AC was lower than 200 μg/mL (Figure 1A). However, we found that pretreatment with 200 μg/mL AC before radiation significantly increased survival rates of E.coli as compared to that treated with radiation alone at 50 J/m2 doses (Figure 1B). Similarly, AC administration increased cell viability of H2O2-treated E.coli (Figure 1C). These data imply that the AC-mediated enhancement of E.coli cell viability might result in inhibition of LPS release, and subsequently the attenuation of the lung injury.

Figure 1 Effect of AC on cell viability of radiation or H2O2-treated E.coli. (A) The cytotoxicity of different concentrations of AC against E.coli.(B) The effect of AC on cell viability induced by 50 J/m2 doses radiation. (C) Variation of E.coli survival pretreated with 200 μg/mL AC with the treatment of H2O2. **, P<0.01.
Figure 1

Effect of AC on cell viability of radiation or H2O2-treated E.coli. (A) The cytotoxicity of different concentrations of AC against E.coli.(B) The effect of AC on cell viability induced by 50 J/m2 doses radiation. (C) Variation of E.coli survival pretreated with 200 μg/mL AC with the treatment of H2O2. **, P<0.01.

3.2 AC enhances cell viability of fibroblasts treated with radiation or H2O2

Fibroblasts, the pivotal cell type in lung tissue, have a key role in protecting against lung injury. Dysfunction of fibroblasts leads to lung injury [11]. As shown in Figure 2A, AC reduced proliferation and diminished the viability of fibroblasts when the concentration of AC was more than 400 μg/mL, whereas lower than 200 μg/ mL did not affect cell viability of fibroblasts. However, pretreatment of fibroblasts with 200 μg/mL AC before radiation significantly promoted survival rates compared to fibroblasts treated with radiation alone at 6 Gy doses (Figure 2B). Similarly, AC enhanced H2O2 treatment-mediated decline in fibroblast cell viability (Figure 2C). These data suggest that AC attenuates cell apoptosis in irradiated fibroblasts.

Figure 2 Effect of AC on cell viability of radiation or H2O2-treated fibroblasts. (A) The cytotoxicity of different concentrations of AC against fibroblasts. (B) The effect of AC on cell viability induced by 50 J/m2 doses radiation. (C) Variation of fibroblasts survival pretreated with 200 μg/mL AC with the treatment of H2O2. **, P<0.01.
Figure 2

Effect of AC on cell viability of radiation or H2O2-treated fibroblasts. (A) The cytotoxicity of different concentrations of AC against fibroblasts. (B) The effect of AC on cell viability induced by 50 J/m2 doses radiation. (C) Variation of fibroblasts survival pretreated with 200 μg/mL AC with the treatment of H2O2. **, P<0.01.

3.3 AC attenuates DNA damage in radiated fibroblasts

Next, we determined the effect of AC on DNA damage in fibroblasts. In our study, we discovered that irradiated fibroblasts resulted in an increase in the number of all comet tail moment, whereas pretreatment with AC before radiation clearly inhibited the increase of comet tail moment, indicating that AC had a protective effect on radiation-induced DNA damage (Figure 3A and 3B). In addition, AC attenuated the apoptosis of irradiated fibroblasts with a decrease in cleaved PARP compared to radiation treatment alone (Figure 3C).

Figure 3 Effect of AC on radiation-induced DNA damage in fibroblasts. (A) Representative micrographs of comet assay. (B) Relative comet tail rate (%). (C) Protein expression of full-length PARP, Cleaved PARP and Actin by WB. **, P<0.01.
Figure 3

Effect of AC on radiation-induced DNA damage in fibroblasts. (A) Representative micrographs of comet assay. (B) Relative comet tail rate (%). (C) Protein expression of full-length PARP, Cleaved PARP and Actin by WB. **, P<0.01.

3.4 AC improves survival rates of irradiated mice

We also evaluated the role of AC on radiation-evoked injuries in vivo. As shown in Figure 4A, lung tissue was severely damaged accompanied by increased fibrosis after radiation treatment. Once treated with AC, radiation-induced lung injury was significantly improved and the fibrosis level was obviously decreased (Figure 4A). Consistent with the previous study, we also observed that all irradiated mice died on day 10 after irradiation alone, while 50% of the mice survived day 10 after radiation when treated with 25 mg/kg AC (Figure 4B). These data show that AC improves the overall survival rates of irradiated mice.

Figure 4 Effect of AC on radiation-induced lung fibrosis and mice death. (A) Histologic results of lung tissue in different group measured by HE staining. Scale bar: 200 μm. (B) Survival rates of radiated mice with or without AC treatment for 15 days.
Figure 4

Effect of AC on radiation-induced lung fibrosis and mice death. (A) Histologic results of lung tissue in different group measured by HE staining. Scale bar: 200 μm. (B) Survival rates of radiated mice with or without AC treatment for 15 days.

3.5 AC increases the levels of plasma T-AOC and inhibits the expression of TGF-β1

Radiation can induce lung injury and even pulmonary fibrosis. Elevated levels of TGF-β1 indicate the onset of pulmonary fibrosis [12]. Interestingly, we found that TGF-β1 mRNA was significantly increased with the passage of time under the condition of irradiation compared to the control, showing peak effect at 8 weeks which suggested that our in vitro model was successfully established (Figure 5A). To explore the role of AC on radiation-mediated elevation of TGF-β1, we also chose 200 μg/mL as the treatment concentration. Like previous results, protein and mRNA levels of TGF-β1 in mice was robustly boosted after irradiation, whereas it was significantly reduced followed by the treatment of 25 mg/kg AC (Figure 5B and 5C). Additionally, plasma T-AOC concentrations in the AC-treated group were significantly higher than those in the group treated with irradiation alone. Changes in the levels of T-AOC indicate the antioxidant potential of AC (Figure 5D).

Figure 5 Levels of Plasma T-AOC (a marker of oxidative stress) and the expression of TGF-β1. (A and B) The level of TGF-β1 mRNA. (C) Observation of the expression of TGF-β1 by WB. (D) Levels of plasma T-AOC. **, P<0.01.
Figure 5

Levels of Plasma T-AOC (a marker of oxidative stress) and the expression of TGF-β1. (A and B) The level of TGF-β1 mRNA. (C) Observation of the expression of TGF-β1 by WB. (D) Levels of plasma T-AOC. **, P<0.01.

In summary, AC administration improves irradiation-mediated DNA damage and growth inhibition of fibroblasts. At the same time, AC enhances the level of T-AOC and inhibits the level of TGF-β1 in mice after irradiation, thereby prolonging survival. It appears that AC could serve as an effective and safe radio-protective agent.

4 Discussion

A side effect of radiation therapy is the generation of ROS that can result in oxidative deterioration of proteins and of DNA [13]. Elevated levels of ROS cause DNA damage, lung injuries and even pulmonary fibrosis [14]. It is known that antioxidants have an effective role in ameliorating radiation-induced oxidative injuries [15]. AC has been known to have anti-oxidant activity, however, the effect of AC on the progression of radiation-induced injury has not been studied [16]. In our study, we showed that AC increased the survival rates of irradiated or H2O2-treated cells, consistent with the previous study. AC attenuates radiation-induced injury, which may result from their radical-scavenging effect.

Previously, it has shown that acute exposure to radiation induces tissues injuries and mouse death [17]. Radiation also causes an increase in the expression of TGF-β1 to induce pulmonary fibrosis, resulting in cell apoptosis [18]. Similarly, the level of TGF-β1 in radiation-treated mice is significantly increased, and AC gavage significantly reduced the expression of TGF-β1 in mice. Continuous monitoring of radiation-treated mice for 15 days revealed that radiation caused a severe decline in survival rates of mice, and irradiated mice treated with AC significantly prolonged mouse survival, which indicates AC could be a potential radio-protective agent.

It is known that radiation induces DNA damage such as DNA double-strand breaks (DSBs) which are major cytotoxic lesions and pose a significant threat to genome stability if not properly repaired [19, 20]. Radiation induces large amounts of ROS targeting membrane lipids and DNA [21, 22]. DNA damage is one factor causing apoptosis of the cells [6, 23]. In our study, we found that AC significantly restored the level of serum T-AOC caused by radiation, and mitigated radiation-induced DNA damage, indicating that AC might act as a radio-protectant for the treatment of side effects produced by radiation therapy. Additionally, pretreatment of AC prior to radiation appeared to have a protective role against cell apoptosis and mouse death. Thus, the beneficial role of AC in radiation-induced damage is involvement with the cellular process of DNA damage and cell apoptosis.

In conclusion, AC has a radio-protective effect in vitro and in vivo possibly through its anti-oxidant power. However, the exact mechanism remains to be revealed in future.

Acknowledgments

This study was supported by Science and Technology Project of Zhejiang Translational Chinese Medicine (2017ZA100).

  1. Conflict of interest: Authors state no conflict of interest.

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Received: 2019-01-21
Accepted: 2019-08-21
Published Online: 2020-04-06

© 2020 Haiyan Shen et al. published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
  89. Testosterone aggravates cerebral vascular injury by reducing plasma HDL levels
  90. Bioengineering and Biotechnology
  91. PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect
  92. The thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
  93. Silver nanoparticles: synthesis, characterisation and biomedical applications
  94. Food Science
  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
  98. Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
https://doi.org/10.1515/biol-2020-0015
Schlagwörter für diesen Artikel
Asiaticoside (AC); Radiation-induced injuries (RII); Antioxidant; DNA damage; Radio-protective
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Plant Sciences
  2. Dependence of the heterosis effect on genetic distance, determined using various molecular markers
  3. Plant Growth Promoting Rhizobacteria (PGPR) Regulated Phyto and Microbial Beneficial Protein Interactions
  4. Role of strigolactones: Signalling and crosstalk with other phytohormones
  5. An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
  6. Functional divergence and adaptive selection of KNOX gene family in plants
  7. In silico identification of Capsicum type III polyketide synthase genes and expression patterns in Capsicum annuum
  8. In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)
  9. CRISPR/Cas9 or prime editing? – It depends on…
  10. Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach
  11. Natural variation in stress response induced by low CO2 in Arabidopsis thaliana
  12. The complete mitogenome sequence of the coral lily (Lilium pumilum) and the Lanzhou lily (Lilium davidii) in China
  13. Ecology and Environmental Sciences
  14. Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
  15. Analysis of ethanol dehydration using membrane separation processes
  16. Activity of Vip3Aa1 against Periplaneta americana
  17. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation
  18. Spatiotemporal dynamics of terrestrial invertebrate assemblages in the riparian zone of the Wewe river, Ashanti region, Ghana
  19. Antifungal activity of selected volatile essential oils against Penicillium sp.
  20. Toxic effect of three imidazole ionic liquids on two terrestrial plants
  21. Biosurfactant production by a Bacillus megaterium strain
  22. Distribution and density of Lutraria rhynchaena Jonas, 1844 relate to sediment while reproduction shows multiple peaks per year in Cat Ba-Ha Long Bay, Vietnam
  23. Biomedical Sciences
  24. Treatment of Epilepsy Associated with Common Chromosomal Developmental Diseases
  25. A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
  26. Morphine modulates hippocampal neurogenesis and contextual memory extinction via miR-34c/Notch1 pathway in male ICR mice
  27. Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil
  28. Weight loss may be unrelated to dietary intake in the imiquimod-induced plaque psoriasis mice model
  29. Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
  30. Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2
  31. Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
  32. Prevalence of dyslipidemia in Tibetan monks from Gansu Province, Northwest China
  33. Sevoflurane inhibits proliferation, invasion, but enhances apoptosis of lung cancer cells by Wnt/β-catenin signaling via regulating lncRNA PCAT6/ miR-326 axis
  34. MiR-542-3p suppresses neuroblastoma cell proliferation and invasion by downregulation of KDM1A and ZNF346
  35. Calcium Phosphate Cement Causes Nucleus Pulposus Cell Degeneration Through the ERK Signaling Pathway
  36. Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential
  37. MiR-489-3p inhibits cell proliferation, migration, and invasion, and induces apoptosis, by targeting the BDNF-mediated PI3K/AKT pathway in glioblastoma
  38. Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating the microRNA-34a-5p/NOTCH1 signaling pathway
  39. Large Brunner’s gland adenoma of the duodenum for almost 10 years
  40. Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing
  41. Hepatoprotective effects of chamazulene against alcohol-induced liver damage by alleviation of oxidative stress in rat models
  42. FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis
  43. Risk factors for elevated serum colorectal cancer markers in patients with type 2 diabetes mellitus
  44. Effect of hepatic sympathetic nerve removal on energy metabolism in an animal model of cognitive impairment and its relationship to Glut2 expression
  45. Progress in research on the role of fibrinogen in lung cancer
  46. Advanced glycation end product levels were correlated with inflammation and carotid atherosclerosis in type 2 diabetes patients
  47. MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB
  48. Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia
  49. Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  50. Effect of FibroScan test in antiviral therapy for HBV-infected patients with ALT <2 upper limit of normal
  51. LncRNA SNHG15 regulates osteosarcoma progression in vitro and in vivo via sponging miR-346 and regulating TRAF4 expression
  52. LINC00202 promotes retinoblastoma progression by regulating cell proliferation, apoptosis, and aerobic glycolysis through miR-204-5p/HMGCR axis
  53. Coexisting flavonoids and administration route effect on pharmacokinetics of Puerarin in MCAO rats
  54. GeneXpert Technology for the diagnosis of HIV-associated tuberculosis: Is scale-up worth it?
  55. Circ_001569 regulates FLOT2 expression to promote the proliferation, migration, invasion and EMT of osteosarcoma cells through sponging miR-185-5p
  56. Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma
  57. BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells
  58. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process
  59. Inhibition of lncRNA LINC00461/miR-216a/aquaporin 4 pathway suppresses cell proliferation, migration, invasion, and chemoresistance in glioma
  60. Upregulation of miR-150-5p alleviates LPS-induced inflammatory response and apoptosis of RAW264.7 macrophages by targeting Notch1
  61. Long non-coding RNA LINC00704 promotes cell proliferation, migration, and invasion in papillary thyroid carcinoma via miR-204-5p/HMGB1 axis
  62. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain
  63. Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages
  64. Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro
  65. MiR-4328 inhibits proliferation, metastasis and induces apoptosis in keloid fibroblasts by targeting BCL2 expression
  66. Aberrant expression of microRNA-132-3p and microRNA-146a-5p in Parkinson’s disease patients
  67. Long non-coding RNA SNHG3 accelerates progression in glioma by modulating miR-384/HDGF axis
  68. Long non-coding RNA NEAT1 mediates MPTP/MPP+-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson’s disease
  69. PCR-detectable Candida DNA exists a short period in the blood of systemic candidiasis murine model
  70. CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway
  71. Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
  72. Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease
  73. The effects of yam gruel on lowering fasted blood glucose in T2DM rats
  74. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis
  75. CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis
  76. Relationship of FTO gene variations with NAFLD risk in Chinese men
  77. The prognostic and predictive value of platelet parameters in diabetic and nondiabetic patients with sudden sensorineural hearing loss
  78. LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis
  79. miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells
  80. LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle
  81. MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression
  82. MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells
  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
  89. Testosterone aggravates cerebral vascular injury by reducing plasma HDL levels
  90. Bioengineering and Biotechnology
  91. PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect
  92. The thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
  93. Silver nanoparticles: synthesis, characterisation and biomedical applications
  94. Food Science
  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
  98. Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
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Heruntergeladen am 26.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/biol-2020-0015/html
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