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LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle

  • Jiangtao Song , Wenrong Song and Lei Zhang EMAIL logo
Published/Copyright: December 22, 2020
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Open Life Sciences
From the journal Open Life Sciences Volume 15 Issue 1

Abstract

A lncRNA RP1-85F18.6 was reported to affect cell growth by regulating the cell cycle. Here we tested whether it affects the proliferation of osteoblast cells by regulating the cell cycle. We determined the expression of RP1-85F18.6 in two osteoblast cell lines hFOB and HOB by qPCR. Then we knocked down or overexpressed RP1-85F18.6 in hFOB and tested the alteration of viability, cell cycle, and cell cycle regulatory proteins. Results showed that both hFOB and HOB expressed RP1-85F18.6. The knockdown of RP1-85F18.6 decreased the viability of hFOB, while the overexpression of it increased the viability. Higher expression of RP1-85F18.6 results in higher cell viability. The knockdown of RP1-85F18.6 caused an increase in the S phase cells and a decrease in the G2/M phase cells. The overexpression of RP1-85F18.6 caused a decrease in the S phase cells and an increase in the G2/M phase cells. The knockdown of RP1-85F18.6 decreased cyclin A, cdk1, E2F, cyclin B, p53, and p21, whereas the overexpression of RP1-85F18.6 increased cyclin A, cdk1, E2F, cyclin B, p53, and p21. This study demonstrated that RP1-85F18.6 is expressed in osteoblast cell lines hFOB and HOB. RP1-85F18.6 affects the proliferation of osteoblasts by regulating the cell cycle.

Keywords: lncRNA; RP1-85F18.6; cell cycle; osteoblast

1 Introduction

Osteoporosis, characterized by significant bone mineral density decrease, is a common metabolic bone disease, which results in fragility fractures or even a fatal outcome. Annually, about 9,000,000 fracture cases are caused by osteoporosis worldwide [1]. Approximately, 30% of women in the United States develop postmenopausal osteoporosis [2], which is the most common bone disease in elderly women [3]. So far, many studies have revealed the pathological mechanisms [4] and developed therapeutics [5,6] for this disease, but more effort is required to further understand osteoporosis.

Long non-coding RNAs (lncRNAs) are RNA transcripts with lengths over 200 nucleotides but are not translated into proteins [7]. Much as the exact functions of lncRNAs remain unclear, they are involved in transcriptional regulation of many genes [8]. The expression of certain non-coding RNAs was reported to impact the bone tissue [9]. Many lncRNAs were also shown to be associated with some human diseases [10,11,12]. Recently, the expression of lncRNA in bone was recognized as a potential osteoporosis risk factor for premenopausal women [13]. Certain lncRNAs were proved to affect bone cells, including osteoblasts [14,15,16]. As the key cells for bone formation, osteoblast cells can differentiate and further form mature bone tissue [17]. Studies revealed that the lncRNAs expressed in bone play a critical role in regulating bone formation [18]. LncRNA H19 was reported to promote differentiation of osteoblast cells [19,20,21]. However, the effect of lncRNAs on the growth of osteoblasts has not been fully elucidated.

A newly identified lncRNA, RP1-85F18.6, which is located on human chromosome 22 [22], was found to affect cell growth by regulating the cell cycle [23]. Here we hypothesized that RP1-85F18.6 affects the proliferation of osteoblasts by regulating the cell cycle. In this study, we tested the expression of RP1-85F18.6 in osteoblast cell lines and determined its effect on the cell cycle. Our study contributed to the development of RP1-85F18.6 as a novel target for the treatment and diagnosis of osteoporosis.

2 Materials and methods

2.1 Cell lines and cell culture

Human colon cancer cell line SW620 [SW-620] (ATCC® CCL-227) and human osteoblast cell line hFOB 1.19 (ATCC® CRL-11372™) were purchased from ATCC (Washington, USA). The primary human osteoblast (HOB) cell line was purchased from PromoCell (Heidelberg, Germany). SW620 was cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, and the other two cell lines were cultured in Osteoblast Growth Medium C-27001 (Heidelberg, Germany) in a humidified atmosphere of 5% CO2 at 37°C.

2.2 MTT

Cell viability was determined by an MTT assay, which was described previously [24]. The cells were cultured in 96-well plates. Then, 20 µL of 5 mg/mL MTT solution (Abcam, Cambridge, UK) was added to the cells. After 2 h, dimethyl sulfoxide (200 µL) was added to the wells. Absorbance was measured at 490 nm using a microplate reader. The experiment was repeated three times, and all the data were normalized and plotted.

2.3 RT-qPCR

The expression of RP1-85F18.6 was determined using a qPCR assay, according to the method that was described previously [25]. TRIzol reagent (Thermo Fisher Scientific, Inc.) was used to isolate total RNA from the cells. Then, the total RNA was reverse-transcribed into cDNA using a reverse transcription kit (Thermo, USA). Quantitative real time-PCR was performed with a PowerUp™ SYBR™ Green Master Mix (Thermo, USA) using a Roche LightCycler 480 Sequence Detection System. The protocol for PCR is as follows: 95°C for 3 min, 40 cycles of 95°C for 30 s, 58°C for 15 s, and 72°C for 30 s. Gene expression was quantified using the 2−ΔΔCT method.

The primers used for RT-qPCR were obtained from Sigma-Aldrich, Inc. (USA), and the sequences are as follows: GAPDH forward, 5′-GAAGGTGAAGGTCGGAGTC-3′; GAPDH reverse, 5′-GAAGATGGTGATGGGATTTC-3′. LncRNA RP1-85F18.6 forward, 5′-GGCTCTTTGCTCACATCG-3′; reverse, 5′-AAGGAAACCACAGGCTCA-3′.

2.4 Cell transfection

The knockdown and overexpression of RP1-85F18.6 were achieved by the transfection of the siRNA or expression vector. The cells were transfected using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), which was described previously [26].

RP1-85F18.6 knockdown: lncRNA RP1-85F18.6 small interfering (si)RNA and negative control (NC) siRNA were purchased from Guangzhou RiboBio Company (Guangzhou, China). The sequences were as follows: lncRNA RP1-85F18.6 siRNA, 5′-GACTCCGCCGTGAACCCTTCA-3′. A scramble siRNA (siN05815122147) was used as the NC siRNA. The transfection concentration of siRNA was 60 nM.

RP1-85F18.6 overexpression: the entire sequence of human lncRNA RP1-85F18.6 was amplified from hFOB cell lines using PCR and cloned into the pcDNA3.1 vector. The NC empty vector, which was purchased from Shanghai GeneChem Co., Ltd (Shanghai, China), and the lncRNA RP1-85F18.6 plasmid were transfected into hFOB using Lipofectamine® 2000. The transfection concentration of the plasmid was 2 µg/mL.

2.5 Cell cycle flow cytometry

The cell cycle was analyzed using flow cytometry with propidium iodide (PI) staining, which was described previously [27]. Briefly, cells were washed with PBS and resuspended at a concentration of 1  ×  106/mL. Then, the cells were fixed with 100% ethanol and incubated for 3 h at 4°C. Then, the suspended cells were washed with PBS twice and added with PI staining solution (0.1% Triton X-100 and 0.2 mg/mL DNAse-free RNAse A, 0.02 mg/mL in cold PBS). Then, the cells were incubated at 37°C for 15 min for staining. A BD FACSCalibur was used to acquire cell cycle data. FlowJo Version 10 was used for the cell cycle analysis.

2.6 Western blotting

Protein expression was determined by a western blotting assay, which was described previously [28]. Proteins were extracted from cells using RIPA buffer with a protease inhibitor (Sigma-Aldrich, USA). Total protein concentrations were determined using a BCA protein assay kit to control the loading amount in each well (30 μg). SDS gel electrophoresis was performed to separate proteins, and the proteins were transferred onto 0.2 μm polyvinylidene difluoride membranes, which were subsequently blocked with 5% skimmed milk in Tris-buffered saline with 0.5% Tween-20 (TBST). The membranes were then reacted with primary (1:1,000 dilution of BSA) and secondary antibodies (1:3,000 dilution of BSA) subsequently. The band intensities were photographed using a gel imaging system after reaction with ECL reagents. The images were quantified using Quantity One® analysis software version 4.3.0. (Bio-Rad, Hercules, CA, USA). The experiment was repeated more than three times.

The primary antibodies used in the experiment are as follows: anti-cyclin A antibody (sc-271682), anti-cdk1 antibody (ab18), anti-E2F antibody (ab179445), anti-cyclin B antibody (ab72), anti-p53 antibody (ab26), anti-p21 antibody (ab109520), cyclin D1 antibody (#2922), anti-Cdk2 antibody (ab32147), anti-cyclin E1 antibody (ab33911), and anti-Cdk2 antibody (ab32147). The secondary antibodies were all purchased from Sigma-Aldrich (USA).

2.7 Statistical analysis

All the experiments were repeated three times. T-test or one-way ANOVA and Dunnett’s post hoc tests were used to compare the significant difference between the control and experimental groups (p  <  0.05). GraphPad Prism (version 6) was used to plot the figures and calculate statistics.

3 Results

3.1 LncRNA RP1-85F18.6 was expressed in hFOB and HOB

Here we tested the expression of lncRNA RP1-85F18.6 in two osteoblast cell lines hFOB and HOB by a PCR assay. Colon cancer cell line SW620, which was reported to have RP1-85F18.6 expression, was used as the positive control [29]. Results showed that lncRNA RP1-85F18.6 was expressed in both cell lines (Figure 1).

Figure 1 The expression of lncRNA RP1-85F18.6 in osteoblast cell lines (*p  <  0.01). Colon cancer cell line SW620 was used as a positive control.
Figure 1

The expression of lncRNA RP1-85F18.6 in osteoblast cell lines (*p  <  0.01). Colon cancer cell line SW620 was used as a positive control.

3.2 The knockdown and overexpression of RP1-85F18.6 in hFOB

To further explore the role of RP1-85F18.6 in osteoblasts, we selected one of the osteoblast cell lines hFOB, which has a higher expression of RP1-85F18.6, to conduct the subsequent study. We knocked down or overexpressed RP1-85F18.6 in hFOB and confirmed the expression by a PCR assay. Results showed that compared to blank control (control), the expressions of RP1-85F18.6 in NC for silencing RNA (NC siRNA) and NC for overexpression (NC overexpress) were not significantly different, while the expression in RP1-85F18.6 knockdown cells (si-RP1-85F18.6) was significantly decreased, and the expression in RP1-85F18.6 overexpressing cells (over-RP1-85F18.6) was significantly increased. This indicated that both the knockdown and overexpression were successful (Figure 2).

Figure 2 The knockdown and overexpression of RP1-85F18.6 (*p  <  0.01).
Figure 2

The knockdown and overexpression of RP1-85F18.6 (*p  <  0.01).

3.3 LncRNA RP1-85F18.6 affected cell viability of hFOB

To test whether RP1-85F18.6 affects cell proliferation, we compared the viability of knocked-down cells, overexpressing cells, and wild-type osteoblasts using an MTT assay. MTT assay has been widely used in cell proliferation study [37]. Results showed that, compared to the control, the expressions of RP1-85F18.6 in NC siRNA and NC overexpress were not significantly different, indicating that the transfection had no effect on cell viability. The knockdown of RP1-85F18.6 significantly decreased cell viability and the overexpression of RP1-85F18.6 significantly increased cell viability (Figure 3). These results indicated that the expression of RP1-85F18.6 was associated with cell viability.

Figure 3 Effect of the expression of RP1-85F18.6 on the viability of osteoblasts (*p  <  0.01).
Figure 3

Effect of the expression of RP1-85F18.6 on the viability of osteoblasts (*p  <  0.01).

3.4 LncRNA RP1-85F18.6 affected the cell cycle of hFOB

To further explore the effect of RP1-85F18.6 on cell growth of osteoblasts, we analyzed the cell cycle of hFOB cells using a flow cytometry assay with PI staining. Results showed that compared to the control, the cell cycles of NC siRNA and NC overexpress were not significantly different, indicating that the transfection had no effect on the cell cycle. The knockdown of RP1-85F18.6 had no effect on the G0/G1 phase cells but caused a significant increase in the S phase cells and a decrease in the G2/M phase cells. On the other hand, the overexpression of RP1-85F18.6 also had no significant effect on the G0/G1 phase cells but caused a decrease in the S phase cells and an increase in the G2/M phase cells. This indicated that the expression of RP1-85F18.6 affected osteoblast cells in the S and G2/M phase (Figure 4). Therefore, we suggested that RP1-85F18.6 promotes cell growth by regulating the cell cycle.

Figure 4 The cell cycle of cells was determined by a flow cytometry assay with PI staining (significant differences from NC siRNA and NC overexpress are indicated by *p  <  0.05 and Δp  <  0.05, respectively).
Figure 4

The cell cycle of cells was determined by a flow cytometry assay with PI staining (significant differences from NC siRNA and NC overexpress are indicated by *p  <  0.05 and Δp  <  0.05, respectively).

3.5 LncRNA RP1-85F18.6 affected the expression of cell cycle-related proteins in hFOB

To identify the potential targets involved in the effect of RP1-85F18.6 on the cell cycle, we determined several cell cycle-related proteins by using western blotting. First, we determined the expression of cyclin A, cdk1, and E2F proteins. Cyclin A and cdk1 are responsible for S phase regulation [30]. Results showed that the transfection had no effect on the expression of all the proteins studied, whereas the knockdown of RP1-85F18.6 decreased the expression of cyclin A, cdk1, and E2F. The overexpression of RP1-85F18.6 did the opposite: it increased the expression of cyclin A, cdk1, and E2F (Figure 5). Then, we determined the expression of cyclin B, p53, and p21 proteins. The p53/21 pathway is responsible for the negative regulation of cyclin B and cdk1, regulating activities of the G2 phase [31]. Results showed that the transfection had no effect on the expression of all the proteins studied (all p > 0.05). The knockdown of RP1-85F18.6 decreased the expression of cyclin B, p53, and p21 (all p < 0.01), whereas the overexpression of RP1-85F18.6 increased the expression of cyclin B, p53, and p21 (all p < 0.01) (Figure 6). Moreover, we also determined the expression of cyclin D, cdk4, cyclin E, and cdk2 proteins. Cyclin D and cyclin E are the regulators for cell activities in the G1 phase [32]. Results showed that the expressions of cyclin D, cdk4, cyclin E, and cdk2 in control, NC siRNA, si-RP1-85F18.6, NC overexpress, and over-RP1-85F18.6 groups were not significantly different (Figure 7). Therefore, RP1-85F18.6 might promote cell growth by regulating cyclin proteins. We proposed a mechanism for the regulation of RP1-85F18.6, which is discussed in the Discussion section (Figure 8).

Figure 5 Effect of lncRNA RP1-85F18.6 on the expression of cyclin A related proteins (significant differences are indicated by *p  <  0.01).
Figure 5

Effect of lncRNA RP1-85F18.6 on the expression of cyclin A related proteins (significant differences are indicated by *p  <  0.01).

Figure 6 Effect of lncRNA RP1-85F18.6 on the expression of cyclin B related proteins (*p  <  0.01).
Figure 6

Effect of lncRNA RP1-85F18.6 on the expression of cyclin B related proteins (*p  <  0.01).

Figure 7 Effect of lncRNA RP1-85F18.6 on the expression of cyclin D and cyclin E related proteins (*p  <  0.05).
Figure 7

Effect of lncRNA RP1-85F18.6 on the expression of cyclin D and cyclin E related proteins (*p  <  0.05).

Figure 8 Mechanism of the effect of RP1-85F18.6 on the cell cycle.
Figure 8

Mechanism of the effect of RP1-85F18.6 on the cell cycle.

4 Discussion

This study tried to reveal the role of a newly discovered lncRNA RP1-85F18.6 in the proliferation of osteoblasts. We confirmed that RP1-85F18.6 was expressed in both adult (HOB) and fetus (hFOB) derived osteoblast cell lines. These two cell lines are different in their capability of proliferation and differentiation, but it is unknown whether this is associated with their difference in RP1-85F18.6 expression levels. In this study, we focused only on one of these cell lines, hFOB. The reason for selecting hFOB is that hFOB has higher expression of RP1-85F18.6, and it is a stable cell line for transfection [33]. Previously, RP1-85F18.6 was reported to be knocked down and overexpressed in cells successfully [34]. In our study, the expression of RP1-85F18.6 was closely related to the cell viability of hFOB, suggesting that RP1-85F18.6 might promote cell growth.

RP1-85F18.6 was reported to increase colon cancer cell growth [34], and we assumed that the osteoblast and colon cancer cells might share a common mechanism. Cell cycle analysis showed that RP1-85F18.6 expression affected cell viability through regulating the S and G2/M phases. The G0/G1 phase of the cell cycle is the period for differentiation of osteoblasts [35]. The S phase entrance is critical for cell proliferation, and the arrest of cells in the S phase can result in the suppression of proliferation [36]. To explore the effect of RP1-85F18.6 on the action of the cell cycle, we selected and determined the expression of several cell cycle regulatory proteins. The cell cycle is regulated by cyclins, cyclin-dependent kinases (CDKs), and some transcription factors. Cyclins/CDK complexes are the most critical regulators for cell cycle regulation [35]. In this study, we determined the expression of four pairs of cyclins/CDK complexes, which regulate physical processes of each phase in cell cycle, and also some of their upstream regulators.

E2F is a protein that can activate genes required for DNA synthesis and S phase progression. The cyclin A and cdk1, downstream targets of E2F, are responsible for S phase regulation [30]. In this study, the knockdown of RP1-85F18.6 decreased the expression of E2F and further resulted in the decrease of cyclin A and cdk1, whereas the overexpression of RP1-85F18.6 increased the expression of E2F, which accounts for the increase of cyclin A and cdk1.

Besides, the p53/21 pathway is responsible for the negative regulation of cyclin B and cdk1, regulating activities of the G2 phase in cancer cells [34]. The accumulation of p53 can increase the expression of its transcriptional target gene p21, which can potentially inactivate the cyclin B/cdk1 complex [31]. Our results showed that the knockdown or overexpression of RP1-85F18.6 affects the expression of p53, and p53 subsequently affected p21, which negatively regulated activities in the G2 phase, but the increase of cyclin B and cdk1 offset the effect of p21 suppression, and thus the knockdown of RP1-85F18.6 can arrest cells in the S phase, and overexpression of RP1-85F18.6 can promote cells entering the G2 phase. Nevertheless, this study did not demonstrate how they regulate the proliferation in detail, but it provides a potential mechanism that can be validated in the future.

Furthermore, cyclin D and cyclin E are the regulators for cell activities in the G1 phase [32]. Our results showed that the expression of cyclin D, cdk4, cyclin E, and cdk2 was not affected by RP1-85F18.6, which was consistent with the results that the number of cells in the G1/G0 phase was not changed. Besides, p21 can also potentially bind and inactivate the cyclin E/CDK2 complex. This might also account for the change in the number of cells entering the S phase from the G1 phase. However, it is still not clear why the increase of p21 failed to affect cell proliferation, and we supposed a feedback regulation was involved (Figure 8).

In summary, this study demonstrated that lncRNA RP1-85F18.6 plays a role in the proliferation of osteoblasts by affecting their cell cycle by regulating several cell cycle proteins. We suggested that RP1-85F18.6 is an upstream target of cell cycle-related proteins, but whether its regulation is direct or indirect is still unclear and requires more exploration. Additionally, lncRNA H19 was reported to promote osteoblast differentiation [19,20,21]. Further study is needed to explore its effect on the differentiation of osteoblasts. In addition, there might be some linkage between osteoporosis and the decrease of other lncRNAs, which requires more validation. Our study is conducive to the development of RP1-85F18.6 as a novel treatment target for osteoporosis.

Acknowledgments

This work was supported by grants from the Hanchuan People’s Hospital, China.

  1. Conflict of interest: The authors state no conflict of interest.

  2. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Received: 2020-04-16
Revised: 2020-07-01
Accepted: 2020-07-21
Published Online: 2020-12-22

© 2020 Jiangtao Song et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  1. Plant Sciences
  2. Dependence of the heterosis effect on genetic distance, determined using various molecular markers
  3. Plant Growth Promoting Rhizobacteria (PGPR) Regulated Phyto and Microbial Beneficial Protein Interactions
  4. Role of strigolactones: Signalling and crosstalk with other phytohormones
  5. An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
  6. Functional divergence and adaptive selection of KNOX gene family in plants
  7. In silico identification of Capsicum type III polyketide synthase genes and expression patterns in Capsicum annuum
  8. In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)
  9. CRISPR/Cas9 or prime editing? – It depends on…
  10. Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach
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  12. The complete mitogenome sequence of the coral lily (Lilium pumilum) and the Lanzhou lily (Lilium davidii) in China
  13. Ecology and Environmental Sciences
  14. Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
  15. Analysis of ethanol dehydration using membrane separation processes
  16. Activity of Vip3Aa1 against Periplaneta americana
  17. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation
  18. Spatiotemporal dynamics of terrestrial invertebrate assemblages in the riparian zone of the Wewe river, Ashanti region, Ghana
  19. Antifungal activity of selected volatile essential oils against Penicillium sp.
  20. Toxic effect of three imidazole ionic liquids on two terrestrial plants
  21. Biosurfactant production by a Bacillus megaterium strain
  22. Distribution and density of Lutraria rhynchaena Jonas, 1844 relate to sediment while reproduction shows multiple peaks per year in Cat Ba-Ha Long Bay, Vietnam
  23. Biomedical Sciences
  24. Treatment of Epilepsy Associated with Common Chromosomal Developmental Diseases
  25. A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
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  28. Weight loss may be unrelated to dietary intake in the imiquimod-induced plaque psoriasis mice model
  29. Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
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  45. Progress in research on the role of fibrinogen in lung cancer
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  49. Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  50. Effect of FibroScan test in antiviral therapy for HBV-infected patients with ALT <2 upper limit of normal
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  57. BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells
  58. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process
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  61. Long non-coding RNA LINC00704 promotes cell proliferation, migration, and invasion in papillary thyroid carcinoma via miR-204-5p/HMGB1 axis
  62. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain
  63. Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages
  64. Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro
  65. MiR-4328 inhibits proliferation, metastasis and induces apoptosis in keloid fibroblasts by targeting BCL2 expression
  66. Aberrant expression of microRNA-132-3p and microRNA-146a-5p in Parkinson’s disease patients
  67. Long non-coding RNA SNHG3 accelerates progression in glioma by modulating miR-384/HDGF axis
  68. Long non-coding RNA NEAT1 mediates MPTP/MPP+-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson’s disease
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  70. CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway
  71. Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
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  74. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis
  75. CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis
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  77. The prognostic and predictive value of platelet parameters in diabetic and nondiabetic patients with sudden sensorineural hearing loss
  78. LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis
  79. miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells
  80. LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle
  81. MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression
  82. MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells
  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
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  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
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  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
https://doi.org/10.1515/biol-2020-0090
Keywords for this article
lncRNA; RP1-85F18.6; cell cycle; osteoblast
Creative Commons
BY 4.0

Articles in the same Issue

  1. Plant Sciences
  2. Dependence of the heterosis effect on genetic distance, determined using various molecular markers
  3. Plant Growth Promoting Rhizobacteria (PGPR) Regulated Phyto and Microbial Beneficial Protein Interactions
  4. Role of strigolactones: Signalling and crosstalk with other phytohormones
  5. An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
  6. Functional divergence and adaptive selection of KNOX gene family in plants
  7. In silico identification of Capsicum type III polyketide synthase genes and expression patterns in Capsicum annuum
  8. In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)
  9. CRISPR/Cas9 or prime editing? – It depends on…
  10. Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach
  11. Natural variation in stress response induced by low CO2 in Arabidopsis thaliana
  12. The complete mitogenome sequence of the coral lily (Lilium pumilum) and the Lanzhou lily (Lilium davidii) in China
  13. Ecology and Environmental Sciences
  14. Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
  15. Analysis of ethanol dehydration using membrane separation processes
  16. Activity of Vip3Aa1 against Periplaneta americana
  17. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation
  18. Spatiotemporal dynamics of terrestrial invertebrate assemblages in the riparian zone of the Wewe river, Ashanti region, Ghana
  19. Antifungal activity of selected volatile essential oils against Penicillium sp.
  20. Toxic effect of three imidazole ionic liquids on two terrestrial plants
  21. Biosurfactant production by a Bacillus megaterium strain
  22. Distribution and density of Lutraria rhynchaena Jonas, 1844 relate to sediment while reproduction shows multiple peaks per year in Cat Ba-Ha Long Bay, Vietnam
  23. Biomedical Sciences
  24. Treatment of Epilepsy Associated with Common Chromosomal Developmental Diseases
  25. A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
  26. Morphine modulates hippocampal neurogenesis and contextual memory extinction via miR-34c/Notch1 pathway in male ICR mice
  27. Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil
  28. Weight loss may be unrelated to dietary intake in the imiquimod-induced plaque psoriasis mice model
  29. Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
  30. Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2
  31. Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
  32. Prevalence of dyslipidemia in Tibetan monks from Gansu Province, Northwest China
  33. Sevoflurane inhibits proliferation, invasion, but enhances apoptosis of lung cancer cells by Wnt/β-catenin signaling via regulating lncRNA PCAT6/ miR-326 axis
  34. MiR-542-3p suppresses neuroblastoma cell proliferation and invasion by downregulation of KDM1A and ZNF346
  35. Calcium Phosphate Cement Causes Nucleus Pulposus Cell Degeneration Through the ERK Signaling Pathway
  36. Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential
  37. MiR-489-3p inhibits cell proliferation, migration, and invasion, and induces apoptosis, by targeting the BDNF-mediated PI3K/AKT pathway in glioblastoma
  38. Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating the microRNA-34a-5p/NOTCH1 signaling pathway
  39. Large Brunner’s gland adenoma of the duodenum for almost 10 years
  40. Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing
  41. Hepatoprotective effects of chamazulene against alcohol-induced liver damage by alleviation of oxidative stress in rat models
  42. FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis
  43. Risk factors for elevated serum colorectal cancer markers in patients with type 2 diabetes mellitus
  44. Effect of hepatic sympathetic nerve removal on energy metabolism in an animal model of cognitive impairment and its relationship to Glut2 expression
  45. Progress in research on the role of fibrinogen in lung cancer
  46. Advanced glycation end product levels were correlated with inflammation and carotid atherosclerosis in type 2 diabetes patients
  47. MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB
  48. Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia
  49. Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  50. Effect of FibroScan test in antiviral therapy for HBV-infected patients with ALT <2 upper limit of normal
  51. LncRNA SNHG15 regulates osteosarcoma progression in vitro and in vivo via sponging miR-346 and regulating TRAF4 expression
  52. LINC00202 promotes retinoblastoma progression by regulating cell proliferation, apoptosis, and aerobic glycolysis through miR-204-5p/HMGCR axis
  53. Coexisting flavonoids and administration route effect on pharmacokinetics of Puerarin in MCAO rats
  54. GeneXpert Technology for the diagnosis of HIV-associated tuberculosis: Is scale-up worth it?
  55. Circ_001569 regulates FLOT2 expression to promote the proliferation, migration, invasion and EMT of osteosarcoma cells through sponging miR-185-5p
  56. Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma
  57. BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells
  58. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process
  59. Inhibition of lncRNA LINC00461/miR-216a/aquaporin 4 pathway suppresses cell proliferation, migration, invasion, and chemoresistance in glioma
  60. Upregulation of miR-150-5p alleviates LPS-induced inflammatory response and apoptosis of RAW264.7 macrophages by targeting Notch1
  61. Long non-coding RNA LINC00704 promotes cell proliferation, migration, and invasion in papillary thyroid carcinoma via miR-204-5p/HMGB1 axis
  62. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain
  63. Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages
  64. Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro
  65. MiR-4328 inhibits proliferation, metastasis and induces apoptosis in keloid fibroblasts by targeting BCL2 expression
  66. Aberrant expression of microRNA-132-3p and microRNA-146a-5p in Parkinson’s disease patients
  67. Long non-coding RNA SNHG3 accelerates progression in glioma by modulating miR-384/HDGF axis
  68. Long non-coding RNA NEAT1 mediates MPTP/MPP+-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson’s disease
  69. PCR-detectable Candida DNA exists a short period in the blood of systemic candidiasis murine model
  70. CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway
  71. Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
  72. Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease
  73. The effects of yam gruel on lowering fasted blood glucose in T2DM rats
  74. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis
  75. CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis
  76. Relationship of FTO gene variations with NAFLD risk in Chinese men
  77. The prognostic and predictive value of platelet parameters in diabetic and nondiabetic patients with sudden sensorineural hearing loss
  78. LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis
  79. miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells
  80. LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle
  81. MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression
  82. MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells
  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
  89. Testosterone aggravates cerebral vascular injury by reducing plasma HDL levels
  90. Bioengineering and Biotechnology
  91. PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect
  92. The thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
  93. Silver nanoparticles: synthesis, characterisation and biomedical applications
  94. Food Science
  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
  98. Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
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