Startseite Antifungal activity of selected volatile essential oils against Penicillium sp.
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Antifungal activity of selected volatile essential oils against Penicillium sp.

  • Soňa Felšöciová , Nenad Vukovic , Paweł Jeżowski und Miroslava Kačániová EMAIL logo
Veröffentlicht/Copyright: 19. Juli 2020
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Open Life Sciences
Aus der Zeitschrift Open Life Sciences Band 15 Heft 1

Abstract

Phytopathogenic fungi have been responsible for considerable economic losses in vineyards, and therefore, more attention should be paid to the development and implementation of preventative treatment that is environmentally friendly. The aim of this study was to evaluate the antifungal activity of ten essential oils (EOs) (viz. Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.). For the antifungal activity evaluation against Penicillium brevicompactum, P. citrinum, P. crustosum, P. expansum, P. funiculosum, P. glabrum, P. chrysogenum, P. oxalicum, P. polonicum and Talaromyces purpurogenus a disc diffusion method was used. The ten EOs exhibited different antifungal properties. Three tested EOs (Carum carvi L., Satureja hortensis L. and Pimpinella anisum L.) at concentrations of 0.75, 0.50, 0.25 and 0.125 µL/mL showed antifungal activity, inhibiting the mycelial growth. The Origanum vulgare L. EOs exhibited a lower level of inhibition. Overall, Lavandula angustifolia Mill., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Pimpinella anisum L. and Rosmarinus officinalis L. were effective as fungicidal agents but their efficiency varied between the strains of fungi. Carum carvi L. showed strong antifungal activity against all tested strains at both full strength and reduced concentrations. These EOs could be considered as potential sources of antifungal compounds for treating plant fungal diseases.

Keywords: antimicrobial activity; disc diffusion method; concentration of plant essential oils; fungi isolated from grape

1 Introduction

Many Penicillium species are soil fungi, while others find their habitat in decaying vegetables, seeds or fruits, which are ecological niches that play a role in the food rotting process. For example P. expansum causes decay of oranges in the citrus industry or rot in grapes [1]. Moreover, these species are known as the major producers of patulin and many other toxic metabolites such as citrinin, roquefortine C or chaetoglobosins among others [2]. Growth of Penicillium in food products is entirely undesirable, especially as many Penicillium species produce mycotoxins and volatile secondary metabolites that are regarded as health hazards and off-flavors [3].

Medicinal plants are sometimes used by different ethnic groups as a natural source of substances used as a cure for diseases of both humans and domestic animals [1]. Some of the plant natural products can have various biological activities such as anti-inflammatory, anticarcinogenic, anti-atherosclerotic, antibacterial, antifungal, antiviral, antimutagenic and antiallergic activities [4,5,6,7,8,9,10,11,12]. The antimicrobial activities of plant extracts have many applications, including raw and processed food preservation, as pharmaceuticals, alternative medicines and natural therapies [13,14].

Essential oils (EOs) are secondary metabolites produced by vascular plants, mostly different species of the labiate family Lamiaceae, Apiaceae and Asteraceae, and other families such as Rutaceae, Lauraceae and Myrtaceae [15].

EOs can be composed of more than 60 components. Phenolic compounds are responsible for the antimicrobial activity of EOs [16]. The effect of EOs on molds can be followed at the macromorphological level, as well as at the cellular level. Some of the macromorphological changes are the lack of sporulation or pigmentation, change in the number of conidia, increased branching of hyphae or change in their size. It has been proposed that some of the mentioned changes are related to the oil activities on enzymatic reactions of cell wall synthesis, which affect mold growth and morphogenesis, and also cause the pulling back of the cytoplasm in hyphae, whereby mycelium death occurs [17]. EOs can inhibit the synthesis of DNA, RNA, proteins and polysaccharides in fungi and bacterial cells, where they can cause changes, similar to the mechanism of antibiotic activity [18,19].

Search for alternative antifungal substances shows the possible use of EOs and extracts for food protection from mycotoxigenic molds and their toxic products [20]. An important role of EOs in nature is protection of plants by acting as antifungal agents. The hypothesis is how different volatile EOs in different concentrations influence different plant fungal strains of Penicillium sp. The aim of this study is to present the antifungal properties of ten EOs against ten Penicillium strains isolated from plants.

2 Materials and methods

2.1 Plant EOs

The EOs used in this study were commercial samples from Calendula a.s., Nová Ľubovňa, Slovakia (Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.). All samples were stored at 4°C in a dark glass flask until analysis. Pure EOs were dissolved in DMSO (dimethylsulfoxide; Penta, Czech Republic) at different concentrations. The 0.75 µL/mL (mass per volume) solutions thus prepared were diluted to 0.375, 0.1875 and 0.09375 µL/mL and immediately analyzed.

2.2 Fungal strains and media

The selected plant fungal strains P. brevicompactum, P. citrinum, P. crustosum, P. expansum, P. funiculosum, P. glabrum, P. chrysogenum, P. oxalicum, P. polonicum and Talaromyces purpurogenus (previously called Penicillium purpurogenum) were obtained from the fungal culture collection bank at the Department of Microbiology, Slovak University of Agriculture in Nitra. Fungal strains were maintained on Czapek yeast extract agar (CYA, HiMedia, Bombay, India), and the cultures were stored at −21°C. Genus Penicillium that was 7 days old was identified to the species level based on macroscopic and microscopic characteristics according to the manuals of Pitt [21], Samson and Frisvad [22] and Samson et al. [23]. After microscopic identification, strains of fungi were confirmed with a MALDI-TOF MS Biotyper (Bruker Daltonics, Bremen, Germany).

2.3 Disc diffusion method

Seven-day-old cultures grown on agar plates (CYA) were used for the preparation of the mold conidia suspensions. Conidia suspensions were prepared in a sterile saline solution. The turbidity of the suspension was adjusted with a spectrophotometer – densilameter II (Erba-Lachema, Brno, Czech Republic) at 530 nm to obtain a final concentration that matches that of a 0.5 McFarland standard. Briefly, 100 µL of spore suspension (0.5 McF) was spread thoroughly all over the surface of Sabouraud dextrose agar (SDA; Hi-Media Laboratory, India) plates. The plates were dried in an air-dry stiller at 60°C until evaporation of residual water. Sterile paper discs (6 mm in diameter; Oxoid, Cambridge, UK) were impregnated with 20 µL of EO containing the test compound at a desired concentration (0.75, 0.50, 0.25 and 0.125 µL/mL/disc) and deposited on the agar surface. The test for antifungal properties of EOs was repeated three times, for each microorganism and each concentration. The Petri dishes were incubated at 25 ± 1°C, for 24 h in a thermostat (Friocell, MMM Medcenter Einrichtungen GmbH, Germany). After 24 h of incubation period, the antifungal agent diffused into the agar and inhibited the germination and growth of the tested microorganism. The diameters of inhibition growth zones were measured as semidiameter (in millimeters). Pure DMSO was used as control for each tested fungus [24].

2.4 Chemical composition of EOs

The chemical composition of EOs (Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.) was determined by gas chromatography/mass spectrometry and is reported elsewhere [25].

2.5 Statistical analysis

For calculating the average values and standard deviation of the obtained data, MS Excel (Microsoft Office Professional Plus 2010, Microsoft, USA) was used. The reported values are the mean values from the tests repeated three times.

3 Results and discussion

The chemical analyses by CG/MS revealed that the main constituents of Lavandula angustifolia Mill. were linalool (39.31%) and linalyl acetate (37.68%), for Carum carvi L., carvone (69.54%) and limonene (21.12%); for Pinus mugo var. pumilio, α-pinene (21.26%); for Mentha piperita L., menthol (28.56%) and menthone (27.39%); for Foeniculum vulgare L., anethole (24.98%); for Pinus sylvestris L., α-pinene (26.15%), camphene (15.51%) and bornyl acetate (14.59%); for Satureja hortensis L., carvacrol (41.23%) and γ-terpinene (32.11%); for Origanum vulgare L., carvacrol (43.26%); for Pimpinella anisum L., anethole (63.25%); and for Rosmarinus officinalis L., 1,4-cineole (21.26%), α-pinene (15.65%) and p-cymene (13.28%) [24].

The antifungal properties of different EOs against the growth of Penicillium brevicompactum on SDA are presented in Table 1. The EOs of Lavandula angustifolia Mill. showed strong antifungal activity against Penicillium brevicompactum with a zone of inhibition ranging from 10.33 ± 3.67 to 19.67 ± 0.82 mm. The EOs of Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L. and Rosmarinus officinalis L. exhibited the least antifungal activity with an inhibition zone from 0.17 ± 0.41 to 3.17 ± 0.41 mm against P. brevicompactum. The EO concentration of 0.75 µL/mL showed the most effective inhibition of the growth of Penicillium brevicompactum, followed by 0.50, 0.25 and 0.125 µL/mL concentrations. According to Felšöciová et al. [24], the EOs of Pimpinella anisum L. exhibited the highest antifungal activity against P. brevicompactum at all observed concentrations (0.75, 0.375, 0.1875 and 0.09375 µL/mL) after incubation for 24 h compared to the control sample. However, the EOs of Pinus mugo var. pumilio exhibited the least antifungal activity in the concentration range from 0.25 ± 0.50 to 3.00 ± 2.16 mm against all ten tested oils. The reported results do not correspond with our observations. In our case, Pinus mugo var. pumilio exhibited strong antifungal properties and Pimpinella anisum L. showed a moderate activity limiting the growth of the mentioned fungus. According to D’Auria et al. [26], lavender oil showed both fungistatic and fungicidal activities against Candida albicans strains. Markovic et al. [27] studied the antifungal activity of thymol and carvacrol on Aspergillus spp. and Penicillium spp., and they found that both thymol and carvacrol have potential antifungal activity, but the susceptibility of Aspergillus spp. was more than that of Penicillium spp.

Table 1

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium brevicompactum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.19.67 ± 0.8215.67 ± 2.9413.50 ± 1.3810.33 ± 3.67
2. Carum carvi L.4.50 ± 1.224.17 ± 0.753.50 ± 0.843.00 ± 0.63
3. Pinus mugo var. pumilio14.33 ± 1.759.33 ± 3.503.50 ± 0.551.33 ± 0.52
4. Mentha piperita L.4.50 ± 0.554.00 ± 0.892.50 ± 0.551.67 ± 0.82
5. Foeniculum vulgare L.3.33 ± 1.032.50 ± 0.551.50 ± 0.551.00 ± 0.00
6. Pinus sylvestris L.3.17 ± 0.412.17 ± 0.751.00 ± 0.630.17 ± 0.41
7. Satureja hortensis L.2.67 ± 0.522.00 ± 0.001.33 ± 0.520.67 ± 0.52
8. Origanum vulgare L.2.83 ± 0.411.67 ± 0.520.83 ± 0.410.25 ± 0.42
9. Pimpinella anisum L.6.17 ± 0.415.50 ± 0.555.00 ± 0,632.67 ± 0.52
10. Rosmarinus officinalis L.2.17 ± 1.171.67 ± 0.821.67 ± 0.741.33 ± 0.52
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The antifungal activities of EOs against Penicillium citrinum are shown in Table 2. The best antifungal activity was found for the EO of Pinus sylvestris L. (from 1.17 ± 0.75 to 9.50 ± 1.41 mm) and the lowest was for Pimpinella anisum L., Rosmarinus officinalis L. and Origanum vulgare L. with a zone of inhibition from 1.00 ± 0.00 to 2.17 ± 0.98 mm. A non-inhibitory effect was observed for Mentha piperita L. and Foeniculum vulgare L. at all concentrations tested. Felšöciová et al. [24] reported that the EO of Pimpinella anisum L. was very active against Penicillium citrinum, but the inhibition zones were not measurable, and also the EO of Origanum vulgare L. had a strong antifungal activity with an inhibition zone ranging from 2.75 ± 0.96 to 12.0 ± 1.83 mm (at concentrations 0.75, 0.375, 0.1875 and 0.09375), which is in contrast to our observations. The EOs of Pinus sylvestris L., Mentha piperita L. and Rosmarinus officinalis L. had the lowest activities (from 0.75 ± 0.50 to 3.25 ± 1.26 mm). These findings are in agreement with previous results except for Pinus sylvestris L., for which the inhibition zone ranged from 1.17 ± 0.75 to 9.50 ± 1.41 mm. Scalas et al. [28] evaluated the antifungal activity of Origanum vulgare (oregano), Pinus sylvestris L. (pine) and Thymus vulgaris (thyme red) EOs against Cryptococcus neoformans clinical strains. All EOs displayed an antifungal activity against the C. neoformans isolate, and the order from the most to the least effective EO is as follows: oregano > pine > thyme EOs. Guynot et al. [29] reported that the volatile fraction of five tested EOs (cinnamon leaf, clove, bay, lemongrass and thyme) had potential antifungal activity against the more common fungi causing spoilage of bakery products (Eurotium amstelodami, E. repens, E. rubrum, Aspergillus flavus, A. niger and Penicillium corylophilum). The same effect was observed by Rodríguez et al. [30], that is, the clove EO totally inhibited all of the tested isolates including two Penicillium species (P. nalgiovense and P. roqueforti). Lis-Balchin and Deans [15] reported that strong antimicrobial activity could be correlated with EOs containing high percentages of monoterpenes, eugenol, cinnamic aldehyde and thymol.

Table 2

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium citrinum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.4.17 ± 0.982.67 ± 0.822.00 ± 1.261.17 ± 0.75
2. Carum carvi L.3.67 ± 0.823.17 ± 0.751.83 ± 0.751.50 ± 0.55
3. Pinus mugo var. pumilio3.50 ± 1.383.67 ± 1.631.50 ± 1.640.50 ± 1.26
4. Mentha piperita L.NENENENE
5. Foeniculum vulgare L.NENENENE
6. Pinus sylvestris L.9.50 ± 1.415.00 ± 1.733.00 ± 1.411.17 ± 0.75
7. Satureja hortensis L.5.83 ± 1.035.33 ± 0.822.67 ± 0.821.33 ± 0.52
8. Origanum vulgare L.1.67 ± 0.521.17 ± 0.411.00 ± 0.001.00 ± 0.00
9. Pimpinella anisum L.2.17 ± 0.982.00 ± 1.102.00 ± 1.101.50 ± 0.98
10. Rosmarinus officinalis L.1.83 ± 0.982.17 ± 1.471.50 ± 0.551.00 ± 1.26
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

EOs which showed the strongest antifungal activity against Penicillium crustosum are Carum carvi L., Foeniculum vulgare L. and Satureja hortensis L. (from 6.17 ± 1.33 to 6.67 ± 3.14 mm) at a concentration of 0.75 µL/mL. Other EOs showed a moderate impact on the growth of the mentioned fungus (Table 3). In our previous study, the best antifungal activity against Penicillium crustosum was shown by Pimpinella anisum L., and a strong inhibition effect was also exhibited at a concentration of 0.75 µL/mL by Chamomilla recutita L. and Thymus vulgaris L. [24]. Origanum vulgare L. EOs showed an excellent antifungal activity against the tested fungus P. crustosum for which the zone of inhibition ranges from 3.00 ± 0.82 mm at a concentration of 0.09375 µL/mL to 12.50 ± 1.73 mm at the highest concentration (0.75 µL/mL). A moderate antifungal effect was shown by the oils of Carum carvi L. and Satureja hortensis L. Similar studies have shown the antifungal activity of some EOs including the study of Zyani et al. [31], who reported the important activity of Origanum compactum, Eugenia caryophyllata and Ocimum basilicum EOs against Penicillium commune, Penicillium chrysogenum and Penicillium expansum. Soidrou et al. [32] have found that Comorian EOs isolated from Piper capense, Piper borbonense and Vetiveria zizanoides have a strong fungicidal activity against fungi decaying wood. Several authors have attributed the antifungal activity of EOs to their major phenolic components [33]. Hassan et al. [34] have shown the important antifungal activity of carvacrol against P. expansum. The antifungal activity of the same component against A. niger, A. flavus, P. citrinum and P. chrysogenum was studied [35].

Table 3

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium crustosum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.4.83 ± 1.174.67 ± 1.372.58 ± 1.281.17 ± 0.41
2. Carum carvi L.6.67 ± 3.145.50 ± 2.433.67 ± 2.661.00 ± 0.00
3. Pinus mugo var. pumilio3.00 ± 0.631.83 ± 0.411.50 ± 0.551.17 ± 0.41
4. Mentha piperita L.3.50 ± 0.841.83 ± 0.981.83 ± 0.411.00 ± 0.00
5. Foeniculum vulgare L.6.17 ± 1.335.33 ± 1.511.33 ± 1.510.83 ± 0.98
6. Pinus sylvestris L.5.83 ± 0.752.17 ± 0.412.00 ± 1.261.00 ± 0.00
7. Satureja hortensis L.6.33 ± 3.143.50 ± 1.522.17 ± 0.411.67 ± 0.52
8. Origanum vulgare L.4.17 ± 1.603.17 ± 0.412.17 ± 0.412.00 ± 0.63
9. Pimpinella anisum L.4.83 ± 1.472.83 ± 0.981.17 ± 0.410.50 ± 0.55
10. Rosmarinus officinalis L.3.67 ± 0.822.67 ± 0.521.83 ± 0.980.83 ± 0.98
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The antifungal effects of the ten tested EOs against Penicillium expansum are presented in Table 4. Penicillium expansum was the most sensitive to the EO of Mentha piperita L. at a concentration of 0.75 µL/mL (9.83 ± 2.56 mm). The weakest inhibitory effect was observed for the EOs of Pinus sylvestris L. and Pinus mugo var. pumilio, for which at a concentration of 0.125 µL/mL there was no inhibitory effect. Plavčič et al. [16] presented that the mint EO, in the case of the disc diffusion method, exhibited antifungal activities against eight tested molds. The largest inhibition for the quantity of 0.5 µL was measured against P. expansum growth (14.33 ± 0.58 mm), which is similar to our measurements. A higher inhibition effect was noticed also against P. expansum growth (15.33 ± 0.58 mm). According to the results of Felšöciová et al. [24], a high antagonistic effect against P. expansum was found in Thymus vulgaris L. and Origanum vulgare L. with an inhibition zone from 3.50 ± 1.25 up to 12.00 ± 1.63 mm, but the best antifungal activity at all concentrations was shown by Pimpinella anisum L. and Chamomilla recutita L. The activities of EOs of Pinus sylvestris L. and Pinus mugo var. pumilio were measured at all concentrations, but with a low zone of inhibition from 0.25 ± 0.50 to 1.75 ± 0.50 mm, which is similar to our studies. The concentration of oregano EO required to inhibit the growth of P. expansum was found to be from 3 to 5%, and the difference in required concentrations might be attributed to the variations in the chemical composition of the oregano EOs used and also the use of different substrates and due to the resisting mode of the fungi against various substances present in EOs [36]. The obtained results in the study demonstrated that three compounds (β-ionone, carvone and 1,8-cineole) have real antifungal potential and they could be used as antifungal agents as well as to significantly reduce (or completely eliminate) the growth of Penicillium expansum during the storage of apples [37].

Table 4

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium expansum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.6.50 ± 1.223.83 ± 0.752. 38 ± 1.170.67 ± 0.52
2. Carum carvi L.4.50 ± 2.435.00 ± 2.283.00 ± 0.631.67 ± 0.82
3. Pinus mugo var. pumilio1.67 ± 0.821.17 ± 0.680.33 ± 0.52NE
4. Mentha piperita L.9.83 ± 2.567.17 ± 2.143.67 ± 1.370.83 ± 0.40
5. Foeniculum vulgare L.3.33 ± 1.513.67 ± 2.252.00 ± 1.260.50 ± 0.55
6. Pinus sylvestris L.1.67 ± 0.822.50 ± 1.381.67 ± 0.820.67 ± 0.52
7. Satureja hortensis L.4.00 ± 1.102.50 ± 1.521.00 ± 0.000.67 ± 0.52
8. Origanum vulgare L.3.83 ± 1.722.83 ± 1.722.50 ± 1.760.50 ± 0.55
9. Pimpinella anisum L.3.50 ± 1.384.50 ± 2.172.67 ± 0.820.33 ± 0.52
10. Rosmarinus officinalis L.3.00 ± 1.262.17 ± 1.171.50 ± 0.841.00 ± 0.89
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The highest antifungal activity against Penicillium funiculosum was observed for the extracts of Lavandula angustifolia Mill. with an inhibition zone from 1.67 ± 0.52 to 4.00 ± 0.63 mm (Table 5). The lowest antifungal activity was measured for the EOs of Pimpinella anisum L. and Origanum vulgare L., for which at a concentration 0.125 µL/mL there was no inhibitory effect. Its oil (LEO) has antimicrobial, antifungal, antioxidant, anti-inflammatory, antidepressant, sedative, hypnotic, analgesic and anticancer activity [38,39]. Its impact on reducing the amount of Candida albicans fungus has been shown in in vitro [40] and clinical studies [41]. Motiejūnaite and Peciulyte [42] determined the fungistatic activity of the volatile fraction of pine oil against fungus species: a strong inhibition effect on the growth of Penicillium funiculosum and Trichoderma viride was reported. The antifungal activity of 15 chemically defined EOs, alone and in mixture, was checked by a microdilution test against isolates of Penicillium funiculosum. Origanum vulgare yielded the lowest minimal inhibition concentration (MIC) values, followed by Salvia sclarea, Ocimum basilicum and Cymbopogon citratus, while Citrus paradisi and Citrus limon were not active. All mixtures showed antifungal activity at lower concentration with respect to MIC values of each EO component, when not in combination [43].

Table 5

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium funiculosum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.4.00 ± 0.633.67 ± 0.522.17 ± 0.751.67 ± 0.52
2. Carum carvi L.3.50 ± 0.553.00 ± 1.101.67 ± 0.821.00 ± 0.00
3. Pinus mugo var. pumilio2.83 ± 0.752.17 ± 0.411.33 ± 0.521.00 ± 0.00
4. Mentha piperita L.1.17 ± 0.412.00 ± 0.002.18 ± 0.400.17 ± 0.41
5. Foeniculum vulgare L.3.67 ± 0.522.50 ± 0.551.83 ± 0.411.00 ± 0.00
6. Pinus sylvestris L.2.33 ± 0.822.00 ± 0.891.83 ± 0.980.83 ± 0.41
7. Satureja hortensis L.3.33 ± 0.523.17 ± 0.411.50 ± 0.551.50 ± 0.55
8. Origanum vulgare L.1.00 ± 0.000.83 ± 0.410.83 ± 0.41NE
9. Pimpinella anisum L.1.17 ± 0.411.00 ± 0.000.50 ± 0.550.50 ± 0.55
10. Rosmarinus officinalis L.3.00 ± 1.103.17 ± 1.331.83 ± 0.981.33 ± 0.52
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The screening results of the ten EOs for their activity against the growth of Penicillium glabrum are shown in Table 6. The EO of Lavandula angustifolia Mill. was very active and the inhibition zone was 15.50 ± 1.38 mm at 0.75 µL/mL concentration. On the other hand, low activity was observed for EOs from Rosmarinus officinalis L., Foeniculum vulgare L., Pinus mugo var. pumilio, Mentha piperita L., Pinus sylvestris L. and Origanum vulgare L., which at 0.125 µL/mL concentration showed no zone of inhibition. However, a number of studies report on a strong antifungal activity of basil EO. Dube et al. [44], using the agar plate method, showed that basil oil at a concentration of 1.5 mL/L inhibited completely the growth of 22 species of molds. The study performed by Lis-Balchin et al. [45] points to a strong antifungal effect of an oil that contained estragole as the main component on the growth of Aspergillus niger, A. ochraceus and Fusarium culmorum.

Table 6

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium glabrum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.15.50 ± 1.3811.83 ± 1.724.17 ± 3.600.17 ± 0.41
2. Carum carvi L.7.50 ± 0.554.50 ± 0.552.83 ± 0.413.00 ± 0.63
3. Pinus mugo var. pumilio0.67 ± 0.820.50 ± 0.55NENE
4. Mentha piperita L.0.83 ± 0.980.50 ± 0.550.17 ± 0.26NE
5. Foeniculum vulgare L.0.50 ± 0.550.50 ± 0.55NENE
6. Pinus sylvestris L.1.00 ± 0.000.33 ± 0.520.25 ± 0.27NE
7. Satureja hortensis L.2.17 ± 1.331.00 ± 1.100.50 ± 0.550.50 ± 0.55
8. Origanum vulgare L.1.33 ± 0.520.67 ± 0.32NENE
9. Pimpinella anisum L.2.50 ± 0.551.67 ± 0.521.20 ± 0.500.50 ± 0.55
10. Rosmarinus officinalis L.0.33 ± 0.41NENENE
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

As presented in Table 7, the EOs have strong to moderate antimicrobial activities against the Penicillium chrysogenum tested. In the present study, Pimpinella anisum L., Satureja hortensis L. and lastly Mentha piperita L. exhibit remarkable antifungal activity against Penicillium chrysogenum. The EO of mint (Mentha piperita L.) was used for the purpose of antifungal activity testing against eight different fungi by Plavsic et al. [16]. The inhibition zone was not observed only when the smallest quantity of EO was applied (0.5 µL) against P. expansum (14.33 ± 0.58 mm). The quantity of 1 µL showed inhibitory activity against all tested molds. When the highest quantity of EO was applied (10 µL), the complete inhibition of A. alternata and A. versicolor growth occurred. The inhibition zone of other species was in the range from 13.67 mm (P. chrysogenum) to 44.67 mm (P. aurantiogriseum). Plavsic et al. [16] concluded that the mint EO had the strongest impact on Eurotium herbariorum, and the weakest on P. chrysogenum. According to Motiejūnaite and Peciulyte [42], P. chrysogenum was the least susceptible to pine oil. Slight antifungal activity of pine oil was shown against Aspergillus niger, A. versicolor and Stachybotrys chartarum. The EO from the plant Satureja hortensis L. showed different antimicrobial activities against Aspergillus niger and Candida albicans [46]. Candida albicans showed moderate sensitivity to the oil’s activity, and Aspergillus niger manifested a strong resistance to this oil. Other studies revealed a new biological activity for S. hortensis L., which is the strong inhibition of aflatoxin production by Aspergillus parasiticus. Carvacrol and thymol, and the effective constituents of S. hortensis L., may be useful in controlling aflatoxin contamination of susceptible crops in the field [47]. Kambiz et al. [48] clearly demonstrate that the alcoholic extract of S. hortensis contains compounds possessing antifungal properties. The alcoholic extract of S. hortensis showed antifungal activity against phytopathogenic fungi [49] and against food spoilage fungi [50]. Therefore, on the basis of the results in previous studies, S. hortensis can be added as a protective agent to various food products [47].

Table 7

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium chrysogenum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.2.50 ± 0.551.33 ± 0.521.17 ± 0.411.00 ± 0.00
2. Carum carvi L.3.83 ± 0.752.83 ± 0.751.67 ± 0.520.83 ± 0.98
3. Pinus mugo var. pumilio3.50 ± 0.552.83 ± 0.751.83 ± 0.750.83 ± 0.41
4. Mentha piperita L.5.50 ± 1.524.17 ± 1.473.17 ± 0.750.50 ± 0.55
5. Foeniculum vulgare L.4.00 ± 0.895.50 ± 2.172.00 ± 1.101.33 ± 1.63
6. Pinus sylvestris L.4.00 ± 0.633.33 ± 0.822.83 ± 0.982.17 ± 0.98
7. Satureja hortensis L.6.50 ± 2.072.00 ± 0.001.67 ± 0.520.17 ± 0.41
8. Origanum vulgare L.3.83 ± 0.412.33 ± 0.821.67 ± 0.520.83 ± 0.98
9. Pimpinella anisum L.6.50 ± 5.215.83 ± 4.624.50 ± 2.051.17 ± 0.41
10. Rosmarinus officinalis L.3.50 ± 0.844.17 ± 1.721.83 ± 0.451.00 ± 0.00
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The obtained results from Tables 8 and 9 demonstrate that the highest antifungal activities of Carum carvi L. and Rosmarinus officinalis L. do not differ against Penicillium oxalicum and P. polonicum. At a concentration of 0.125 µL/mL, Pinus mugo var. pumilio indicated no zone of inhibition compared to the control sample for both tested species, which is similar to Lavandula angustifolia Mill. against P. oxalicum and Pinus sylvestris L. against P. polonicum. The EO of Origanum vulgare L. showed no activity at all against the growth of P. polonicum for any of the used concentrations. The EO of caraway (Carum carvi L.) has a wide application in pharmaceutical and food industries as it possesses antitumor, antiproliferative, antihyperglycemic and antimicrobial activity [51]. The EO of caraway, in the case of the disc diffusion method at concentrations of 0.5, 1, 5 and 10 µL, exhibited antifungal activity against eight tested molds. Inhibitory activity using the smallest quantity (0.5 µL) was recorded against all isolates, and the highest inhibition zone was observed against P. chrysogenum (33.67 mm). By using higher concentrations of caraway EO (1 and 5 µL), a greater antifungal effect was observed on all of the tested molds. Total inhibition was noticed against Eurotium herbariorum when using the highest quantity of oil (10 µL), while the highest inhibition zone was observed against A. versicolor (52 mm), and the lowest against A. niger (28 mm). Helal et al. [52] reported that application of 50 µL of the caraway EO, in the agar diffusion method, did not show inhibition zones against A. flavus, while in the case of A. niger, Penicillium digitatum and P. puberulum, the inhibition zones were 22, 18 and 27 mm, respectively. Baghlou et al. [53] detected in vitro the antifungal effect of Rosmarinus officinalis L. (rosemary) EO against 16 fungal strains of A. niger contaminating various food products and responsible for invasive fungal infection. The colonies of the 16 tested strains of A. niger showed a very weak growth at 0.25% concentration of the EO. From a concentration of 0.50%, they noted complete absence of growth of the 16 tested strains. The R. officinalis L. EO also displayed powerful inhibitory and fungicidal activity against specific Candida strains [54]. In the disc diffusion assay, Hendel et al. [55] tested the effect of rosemary extracts on the growth of the green mold of citrus, Penicillium digitatum, under in vitro conditions. The effect was very strong on spore germination, and the diameter of the inhibition zone was estimated to be 14, 20 and 32.5 mm at the concentrations of 15, 20 and 25 µL/mL, respectively, with the lack of sporulation and sparse mycelium compared to the control. These results support the studies on rosemary as a promising source of preservatives. The antifungal activities of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested by Centeno et al. [56] against strains of Aspergillus flavus and A. ochraceus, since these two species are responsible for accumulating mycotoxins that are common contaminants of cereals and grains. These extracts used at low concentrations could have significant potential for the biological control of fungi in food products.

Table 8

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium oxalicum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.1.00 ± 0.000.92 ± 0.20NENE
2. Carum carvi L.6.00 ± 3.105.33 ± 3.782.17 ± 1.471.33 ± 0.52
3. Pinus mugo var. pumilio1.00 ± 0.000.42 ± 0.49NENE
4. Mentha piperita L.2.83 ± 0.412.33 ± 0.521.17 ± 0.410.50 ± 0.55
5. Foeniculum vulgare L.3.83 ± 1.721.83 ± 0.981.00 ± 1.100.83 ± 0.98
6. Pinus sylvestris L.3.67 ± 1.212.50 ± 1.521.17 ± 0.410.50 ± 0.55
7. Satureja hortensis L.2.58 ± 0.491.67 ± 0.411.17 ± 0.410.75 ± 0.27
8. Origanum vulgare L.0.50 ± 0.550.50 ± 0.550.50 ± 0.550.50 ± 0.55
9. Pimpinella anisum L.2.33 ± 2.581.83 ± 2.041.17 ± 1.330.33 ± 0.52
10. Rosmarinus officinalis L.4.67 ± 1.513.17 ± 1.332.00 ± 1.100.50 ± 0.55
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

Table 9

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Penicillium polonicum

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.2.67 ± 1.031.67 ± 0.820.92 ± 0.580.33 ± 0.52
2. Carum carvi L.5.00 ± 3.743.83 ± 3.133.33 ± 3.131.50 ± 1.76
3. Pinus mugo var. pumilio2.67 ± 0.521.67 ± 0.521.17 ± 0.41NE
4. Mentha piperita L.4.67 ± 0.523.17 ± 0.412.50 ± 0.551.00 ± 0.00
5. Foeniculum vulgare L.2.83 ± 0.410.92 ± 0.580.50 ± 0.550.33 ± 0.52
6. Pinus sylvestris L.1.67 ± 0.821.00 ± 0.550.33 ± 0.52NE
7. Satureja hortensis L.2.50 ± 0.551.67 ± 0.821.17 ± 0.750.67 ± 0.52
8. Origanum vulgare L.NENENENE
9. Pimpinella anisum L.3.67 ± 0.523.17 ± 0.413.00 ± 0.000.50 ± 0.55
10. Rosmarinus officinalis L.5.67 ± 1.033.00 ± 0.632.33 ± 1.211.67 ± 1.00
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

The antifungal activities of various EOs against the growth of Talaromyces purpurogenus (previously Penicillium purpurogenum) are presented in Table 10. The EOs of Satureja hortensis L. and Pinus sylvestris L. are the most effective against this fungus with a zone of inhibition ranging from 1.00 ± 0.00 to 7.67 ± 4.50 mm. The antagonistic effect was not found at 0.125 µL/mL for the oil of Origanum vulgare L. against the tested fungus. The turpentine oil extracted from Pinus sylvestris L. showed a significant antifungal effect on fungal plant pathogens Sclerotinia sclerotiorum, Fusarium oxysporum, Botrytis cinerea, Phytophthora capsici, Alternaria solani and Pythium sp., respectively, but it was more active against bacteria and yeast than fungi, and the antimicrobial activity of the oil increased with an increase of oil concentration in the medium [57].

Table 10

Measured sizes of inhibition zones (in mm) for various EOs at different concentrations (mean ± SD) against Talaromyces purpurogenus

EOConcentration of EO (µL/mL)
0.750.500.250.125
1. Lavandula angustifolia Mill.2.83 ± 1.172.50 ± 0.842.00 ± 0.001.50 ± 0.55
2. Carum carvi L.3.67 ± 1.032.67 ± 0.821.67 ± 0.521.50 ± 0.55
3. Pinus mugo var. pumilio2.33 ± 0.521.33 ± 0.521.33 ± 0.521.17 ± 0.41
4. Mentha piperita L.2.17 ± 1.331.67 ± 0.821.00 ± 0.000.67 ± 0.52
5. Foeniculum vulgare L.3.00 ± 0.892.50 ± 0.551.67 ± 0.821.00 ± 0.00
6. Pinus sylvestris L.4.17 ± 1.834.67 ± 1.372.00 ± 0.891.50 ± 0.55
7. Satureja hortensis L.7.67 ± 4.502.67 ± 0.822.00 ± 0.631.00 ± 0.00
8. Origanum vulgare L.3.00 ± 0.002.50 ± 0.550.83 ± 0.41NE
9. Pimpinella anisum L.2.17 ± 0.751.50 ± 0.551.17 ± 0.411.00 ± 0.00
10. Rosmarinus officinalis L.2.83 ± 0.752.50 ± 0.552.50 ± 0.842.17 ± 0.82
DMSO (negative control)NENENENE

NE – non-inhibitory effect.

4 Conclusion

The presented EOs obtained from the selected plants showed different antifungal activities against Penicillium species, depending on the concentration of the EO used, as well as the type of microorganism. The highest antifungal activity was observed for the Lavandula angustifolia Mill. EO against Penicillium brevicompactum. The zone of inhibition varied between 19.67 ± 0.82 and 10.33 ± 3.67 mm depending on the concentration of the EO. The plant extract of Origanum vulgare L. did not possess any strong antifungal activity. At high doses, all tested oils were active against the tested strains, except Mentha piperita L. and Foeniculum vulgare L. against Penicillium citrinum and Origanum vulgare L. against P. polonicum. Diluted oils proved to be less effective and some of them were even inactive: Lavandula angustifolia Mill., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Origanum vulgare L. and Rosmarinus officinalis L.

tel: +421-376-414-715

Acknowledgments

This work was supported by the grant APVV SK-BY-RD-19-0014 “The formulation of novel compositions and study of the properties of the polysaccharide based edible films and coatings with antimicrobial and antioxidant plant additives”.

  1. Conflict of interest: The authors state no conflict of interest.

  2. Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Received: 2020-03-03
Revised: 2020-05-05
Accepted: 2020-05-06
Published Online: 2020-07-19

© 2020 Soňa Felšöciová et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Artikel in diesem Heft

  1. Plant Sciences
  2. Dependence of the heterosis effect on genetic distance, determined using various molecular markers
  3. Plant Growth Promoting Rhizobacteria (PGPR) Regulated Phyto and Microbial Beneficial Protein Interactions
  4. Role of strigolactones: Signalling and crosstalk with other phytohormones
  5. An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
  6. Functional divergence and adaptive selection of KNOX gene family in plants
  7. In silico identification of Capsicum type III polyketide synthase genes and expression patterns in Capsicum annuum
  8. In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)
  9. CRISPR/Cas9 or prime editing? – It depends on…
  10. Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach
  11. Natural variation in stress response induced by low CO2 in Arabidopsis thaliana
  12. The complete mitogenome sequence of the coral lily (Lilium pumilum) and the Lanzhou lily (Lilium davidii) in China
  13. Ecology and Environmental Sciences
  14. Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
  15. Analysis of ethanol dehydration using membrane separation processes
  16. Activity of Vip3Aa1 against Periplaneta americana
  17. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation
  18. Spatiotemporal dynamics of terrestrial invertebrate assemblages in the riparian zone of the Wewe river, Ashanti region, Ghana
  19. Antifungal activity of selected volatile essential oils against Penicillium sp.
  20. Toxic effect of three imidazole ionic liquids on two terrestrial plants
  21. Biosurfactant production by a Bacillus megaterium strain
  22. Distribution and density of Lutraria rhynchaena Jonas, 1844 relate to sediment while reproduction shows multiple peaks per year in Cat Ba-Ha Long Bay, Vietnam
  23. Biomedical Sciences
  24. Treatment of Epilepsy Associated with Common Chromosomal Developmental Diseases
  25. A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
  26. Morphine modulates hippocampal neurogenesis and contextual memory extinction via miR-34c/Notch1 pathway in male ICR mice
  27. Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil
  28. Weight loss may be unrelated to dietary intake in the imiquimod-induced plaque psoriasis mice model
  29. Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
  30. Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2
  31. Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
  32. Prevalence of dyslipidemia in Tibetan monks from Gansu Province, Northwest China
  33. Sevoflurane inhibits proliferation, invasion, but enhances apoptosis of lung cancer cells by Wnt/β-catenin signaling via regulating lncRNA PCAT6/ miR-326 axis
  34. MiR-542-3p suppresses neuroblastoma cell proliferation and invasion by downregulation of KDM1A and ZNF346
  35. Calcium Phosphate Cement Causes Nucleus Pulposus Cell Degeneration Through the ERK Signaling Pathway
  36. Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential
  37. MiR-489-3p inhibits cell proliferation, migration, and invasion, and induces apoptosis, by targeting the BDNF-mediated PI3K/AKT pathway in glioblastoma
  38. Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating the microRNA-34a-5p/NOTCH1 signaling pathway
  39. Large Brunner’s gland adenoma of the duodenum for almost 10 years
  40. Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing
  41. Hepatoprotective effects of chamazulene against alcohol-induced liver damage by alleviation of oxidative stress in rat models
  42. FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis
  43. Risk factors for elevated serum colorectal cancer markers in patients with type 2 diabetes mellitus
  44. Effect of hepatic sympathetic nerve removal on energy metabolism in an animal model of cognitive impairment and its relationship to Glut2 expression
  45. Progress in research on the role of fibrinogen in lung cancer
  46. Advanced glycation end product levels were correlated with inflammation and carotid atherosclerosis in type 2 diabetes patients
  47. MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB
  48. Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia
  49. Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  50. Effect of FibroScan test in antiviral therapy for HBV-infected patients with ALT <2 upper limit of normal
  51. LncRNA SNHG15 regulates osteosarcoma progression in vitro and in vivo via sponging miR-346 and regulating TRAF4 expression
  52. LINC00202 promotes retinoblastoma progression by regulating cell proliferation, apoptosis, and aerobic glycolysis through miR-204-5p/HMGCR axis
  53. Coexisting flavonoids and administration route effect on pharmacokinetics of Puerarin in MCAO rats
  54. GeneXpert Technology for the diagnosis of HIV-associated tuberculosis: Is scale-up worth it?
  55. Circ_001569 regulates FLOT2 expression to promote the proliferation, migration, invasion and EMT of osteosarcoma cells through sponging miR-185-5p
  56. Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma
  57. BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells
  58. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process
  59. Inhibition of lncRNA LINC00461/miR-216a/aquaporin 4 pathway suppresses cell proliferation, migration, invasion, and chemoresistance in glioma
  60. Upregulation of miR-150-5p alleviates LPS-induced inflammatory response and apoptosis of RAW264.7 macrophages by targeting Notch1
  61. Long non-coding RNA LINC00704 promotes cell proliferation, migration, and invasion in papillary thyroid carcinoma via miR-204-5p/HMGB1 axis
  62. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain
  63. Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages
  64. Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro
  65. MiR-4328 inhibits proliferation, metastasis and induces apoptosis in keloid fibroblasts by targeting BCL2 expression
  66. Aberrant expression of microRNA-132-3p and microRNA-146a-5p in Parkinson’s disease patients
  67. Long non-coding RNA SNHG3 accelerates progression in glioma by modulating miR-384/HDGF axis
  68. Long non-coding RNA NEAT1 mediates MPTP/MPP+-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson’s disease
  69. PCR-detectable Candida DNA exists a short period in the blood of systemic candidiasis murine model
  70. CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway
  71. Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
  72. Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease
  73. The effects of yam gruel on lowering fasted blood glucose in T2DM rats
  74. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis
  75. CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis
  76. Relationship of FTO gene variations with NAFLD risk in Chinese men
  77. The prognostic and predictive value of platelet parameters in diabetic and nondiabetic patients with sudden sensorineural hearing loss
  78. LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis
  79. miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells
  80. LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle
  81. MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression
  82. MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells
  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
  89. Testosterone aggravates cerebral vascular injury by reducing plasma HDL levels
  90. Bioengineering and Biotechnology
  91. PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect
  92. The thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
  93. Silver nanoparticles: synthesis, characterisation and biomedical applications
  94. Food Science
  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
  98. Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
https://doi.org/10.1515/biol-2020-0045
Schlagwörter für diesen Artikel
antimicrobial activity; disc diffusion method; concentration of plant essential oils; fungi isolated from grape
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Plant Sciences
  2. Dependence of the heterosis effect on genetic distance, determined using various molecular markers
  3. Plant Growth Promoting Rhizobacteria (PGPR) Regulated Phyto and Microbial Beneficial Protein Interactions
  4. Role of strigolactones: Signalling and crosstalk with other phytohormones
  5. An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
  6. Functional divergence and adaptive selection of KNOX gene family in plants
  7. In silico identification of Capsicum type III polyketide synthase genes and expression patterns in Capsicum annuum
  8. In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)
  9. CRISPR/Cas9 or prime editing? – It depends on…
  10. Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach
  11. Natural variation in stress response induced by low CO2 in Arabidopsis thaliana
  12. The complete mitogenome sequence of the coral lily (Lilium pumilum) and the Lanzhou lily (Lilium davidii) in China
  13. Ecology and Environmental Sciences
  14. Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
  15. Analysis of ethanol dehydration using membrane separation processes
  16. Activity of Vip3Aa1 against Periplaneta americana
  17. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation
  18. Spatiotemporal dynamics of terrestrial invertebrate assemblages in the riparian zone of the Wewe river, Ashanti region, Ghana
  19. Antifungal activity of selected volatile essential oils against Penicillium sp.
  20. Toxic effect of three imidazole ionic liquids on two terrestrial plants
  21. Biosurfactant production by a Bacillus megaterium strain
  22. Distribution and density of Lutraria rhynchaena Jonas, 1844 relate to sediment while reproduction shows multiple peaks per year in Cat Ba-Ha Long Bay, Vietnam
  23. Biomedical Sciences
  24. Treatment of Epilepsy Associated with Common Chromosomal Developmental Diseases
  25. A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
  26. Morphine modulates hippocampal neurogenesis and contextual memory extinction via miR-34c/Notch1 pathway in male ICR mice
  27. Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil
  28. Weight loss may be unrelated to dietary intake in the imiquimod-induced plaque psoriasis mice model
  29. Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
  30. Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2
  31. Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
  32. Prevalence of dyslipidemia in Tibetan monks from Gansu Province, Northwest China
  33. Sevoflurane inhibits proliferation, invasion, but enhances apoptosis of lung cancer cells by Wnt/β-catenin signaling via regulating lncRNA PCAT6/ miR-326 axis
  34. MiR-542-3p suppresses neuroblastoma cell proliferation and invasion by downregulation of KDM1A and ZNF346
  35. Calcium Phosphate Cement Causes Nucleus Pulposus Cell Degeneration Through the ERK Signaling Pathway
  36. Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential
  37. MiR-489-3p inhibits cell proliferation, migration, and invasion, and induces apoptosis, by targeting the BDNF-mediated PI3K/AKT pathway in glioblastoma
  38. Long non-coding RNA TUG1 knockdown hinders the tumorigenesis of multiple myeloma by regulating the microRNA-34a-5p/NOTCH1 signaling pathway
  39. Large Brunner’s gland adenoma of the duodenum for almost 10 years
  40. Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing
  41. Hepatoprotective effects of chamazulene against alcohol-induced liver damage by alleviation of oxidative stress in rat models
  42. FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis
  43. Risk factors for elevated serum colorectal cancer markers in patients with type 2 diabetes mellitus
  44. Effect of hepatic sympathetic nerve removal on energy metabolism in an animal model of cognitive impairment and its relationship to Glut2 expression
  45. Progress in research on the role of fibrinogen in lung cancer
  46. Advanced glycation end product levels were correlated with inflammation and carotid atherosclerosis in type 2 diabetes patients
  47. MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB
  48. Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia
  49. Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
  50. Effect of FibroScan test in antiviral therapy for HBV-infected patients with ALT <2 upper limit of normal
  51. LncRNA SNHG15 regulates osteosarcoma progression in vitro and in vivo via sponging miR-346 and regulating TRAF4 expression
  52. LINC00202 promotes retinoblastoma progression by regulating cell proliferation, apoptosis, and aerobic glycolysis through miR-204-5p/HMGCR axis
  53. Coexisting flavonoids and administration route effect on pharmacokinetics of Puerarin in MCAO rats
  54. GeneXpert Technology for the diagnosis of HIV-associated tuberculosis: Is scale-up worth it?
  55. Circ_001569 regulates FLOT2 expression to promote the proliferation, migration, invasion and EMT of osteosarcoma cells through sponging miR-185-5p
  56. Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma
  57. BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells
  58. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process
  59. Inhibition of lncRNA LINC00461/miR-216a/aquaporin 4 pathway suppresses cell proliferation, migration, invasion, and chemoresistance in glioma
  60. Upregulation of miR-150-5p alleviates LPS-induced inflammatory response and apoptosis of RAW264.7 macrophages by targeting Notch1
  61. Long non-coding RNA LINC00704 promotes cell proliferation, migration, and invasion in papillary thyroid carcinoma via miR-204-5p/HMGB1 axis
  62. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain
  63. Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages
  64. Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro
  65. MiR-4328 inhibits proliferation, metastasis and induces apoptosis in keloid fibroblasts by targeting BCL2 expression
  66. Aberrant expression of microRNA-132-3p and microRNA-146a-5p in Parkinson’s disease patients
  67. Long non-coding RNA SNHG3 accelerates progression in glioma by modulating miR-384/HDGF axis
  68. Long non-coding RNA NEAT1 mediates MPTP/MPP+-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson’s disease
  69. PCR-detectable Candida DNA exists a short period in the blood of systemic candidiasis murine model
  70. CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway
  71. Reversine and herbal Xiang–Sha–Liu–Jun–Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway
  72. Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease
  73. The effects of yam gruel on lowering fasted blood glucose in T2DM rats
  74. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis
  75. CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis
  76. Relationship of FTO gene variations with NAFLD risk in Chinese men
  77. The prognostic and predictive value of platelet parameters in diabetic and nondiabetic patients with sudden sensorineural hearing loss
  78. LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis
  79. miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells
  80. LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle
  81. MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression
  82. MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells
  83. CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis
  84. Relationship between hemodynamic parameters and portal venous pressure in cirrhosis patients with portal hypertension
  85. Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis
  86. Ropivacaine inhibits proliferation, migration, and invasion while inducing apoptosis of glioma cells by regulating the SNHG16/miR-424-5p axis
  87. CD11b is involved in coxsackievirus B3-induced viral myocarditis in mice by inducing Th17 cells
  88. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis
  89. Testosterone aggravates cerebral vascular injury by reducing plasma HDL levels
  90. Bioengineering and Biotechnology
  91. PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect
  92. The thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
  93. Silver nanoparticles: synthesis, characterisation and biomedical applications
  94. Food Science
  95. Bread making potential of Triticum aestivum and Triticum spelta species
  96. Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations
  97. Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies
  98. Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
  99. Animal Sciences
  100. Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment
  101. Agriculture
  102. Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability
  103. Application of extrusion-cooking technology in hatchery waste management
  104. In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids
  105. Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase
  106. Rapid visual detection of FecB gene expression in sheep
  107. Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves
  108. Effects of center pivot sprinkler fertigation on the yield of continuously cropped soybean
  109. Special Issue On New Approach To Obtain Bioactive Compounds And New Metabolites From Agro-Industrial By-Products
  110. Technological and antioxidant properties of proteins obtained from waste potato juice
  111. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry
  112. Special Issue on Computing and Artificial Techniques for Life Science Applications - Part I
  113. Automatic detection and segmentation of adenomatous colorectal polyps during colonoscopy using Mask R-CNN
  114. The impedance analysis of small intestine fusion by pulse source
  115. Errata
  116. Erratum to “Diagnostic performance of serum CK-MB, TNF-α and hs-CRP in children with viral myocarditis”
  117. Erratum to “MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process”
  118. Erratum to “Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation”
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