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Corylus avellana leaf extract-mediated green synthesis of antifungal silver nanoparticles using microwave irradiation and assessment of their properties

  • Mahsa Eshghi , Asa Kamali-Shojaei , Hamideh Vaghari , Yahya Najian , Zahra Mohebian , Omid Ahmadi and Hoda Jafarizadeh-Malmiri EMAIL logo
Published/Copyright: October 11, 2021
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Green Processing and Synthesis
From the journal Green Processing and Synthesis Volume 10 Issue 1

Abstract

Due to high antimicrobial activity against numerous microorganisms, silver nanoparticles (AgNPs) are being utilized in various areas. Microwave-accelerated AgNPs synthesis using Corylus avellana leaf extract was evaluated. Based on randomly central composite design, 13 mixture solutions containing different amounts of the prepared extract (0.10–0.90 mL) and 1 mM silver nitrate solution (15–25 mL) were prepared and exposed to microwave irradiation for 180 s. Response surface methodology was utilized to evaluate the effects of the two independent variables on particle size and concentration of the synthesized AgNPs, as manifested in the place of broad emission peak (λ max) and its absorbance unit, respectively. Fourier transform infrared spectroscopy analysis indicated that the two hydroxyl and carboxylic acid functional groups with reducing activity existed in the prepared extract. Dynamic light scattering and transmission electron microscopy analyses revealed that the formed spherical AgNPs using optimum amounts of C. avellana leaf extract (0.9 mL) and 1 mM silver nitrate solution (25 mL) had minimum particle size (103.5 nm) and polydispersity index (PDI) (0.209), and maximum concentration (140 ppm) and zeta potential (−21.8 mV). Results indicated that the formed AgNPs had high fungicidal effects against the spoiled fungi of Colletotrichum coccodes and Penicillium digitatum.

Keywords: antifungal activity; Corylus avellana ; microwave irradiation; optimization; silver nanoparticles

1 Introduction

Among the various noble metal nanoparticles (NPs), silver NPs (AgNPs) have attracted distinct attention and application in numerous industries and fields due to their unique properties, especially, their strong antimicrobial activity against various microorganisms such as bacteria and fungi strains [1,2,3]. In fact, AgNPs are known as a new generation of antibiotics. Due to the high surface-to-volume ratio of the AgNPs, these NPs can get easily attached to the microorganism’s membrane and by releasing silver ions can change the membrane permeability and respiratory enzymes’ activities, and cause cell death [4,5]. Green synthesis methods in the fabrication of metal and metal oxide NPs have several advantages compared to the synthesis techniques based on physico-chemical approaches. In fact, green or biological synthesis methods are intensified and clean, and ions reduction, NPs’ formation, and their stabilization are completed in a one-step process using bioactive compounds. Furthermore, the NPs’ biosynthesis procedures are cost effective and environmentally friendly because they do not use chemical solvents and reagents in their synthesis procedures [6,7]. In green metal NPs synthesis approach, many natural biomolecules of plants and their derivatives (i.e., steam, root, leaf, and flower) such as proteins/enzymes, amino acids, polysaccharides, alkaloids, phenolic, alcoholic compounds, and vitamins can be involved in the bioreduction, formation, and stabilization of AgNPs [8].

Hazelnut (Corylus avellana L.) belonging to the Betulaceae family contains high amount of fat which increases its nutritional value. Mono- and polyunsaturated fatty acids are the predominant fatty acids in hazelnut [9]. Several studies have indicated that the aqueous extracts of hazelnut leaf contain 3-, 4-, and 5-caffeoylquinic acids, caffeoyltartaric acid, p-coumaroyltartaric acid, myricetin 3-rhamnoside, quercetin 3-rhamnoside, kaempferol 3-rhamnoside, p-coumaric acid, and myricetin and quercetin derivatives. Most of these bioactive compounds have antibacterial and antioxidant action [10,11]. Furthermore, these components have several hydroxyl and carboxylic groups in their structure which those functional groups can easily reduce metal ions and convert those into their elements, and finally form metal NPs [12]. Leaves of C. avellana, an agro-waste, are found in abundance in Iran, and as already mentioned the C. avellana leaf extract has reducing activity which can reduce silver ions and convert them into AgNPs, which have a strong antibiotic effect on a wide range of antibiotic resistant microorganisms. However, this process needs a long time at room temperature, due to the low concentration of bioreducing agents in the extract. Microwave heating can effectively increase the collision rate of the formed silver elements and seeds, and decrease the synthesis time [2,3]. Therefore, this study focused on (1) preparation of the aqueous extract of hazelnut leaf and determination of its main functional groups; (2) synthesis of AgNPs and optimization of the amounts of hazelnut leaf extract and silver salt solution to fabricate AgNPs with minimum particle size and maximum concentration; and (3) assessment of their antifungal action against Colletotrichum coccodes and Penicillium digitatum, which cause tomato and orange spoilage.

2 Materials and method

2.1 Materials

C. avellana leaves were picked from the hazelnut trees in Tabriz, Iran. AgNO3, a silver salt, was bought from Dr Mojallali (Dr Mojallali Chemical Complex Co., Tehran, Iran). Colloidal standard AgNPs solution, with particle size and concentration of 10 nm and 1,000 ppm, respectively, was obtained from Tecnan-Nanomat (Spain). C. coccodes and P. digitatum were isolated from spoiled tomato and orange. Potato dextrose agar (PDA) was purchased from Oxoid Ltd (Hampshire, United Kingdom). Deionized double distilled water was used to prepare all aqueous solutions.

2.2 Preparation of C. avellana leaf extract and synthesis of AgNPs

The C. avellana leaves were washed, shade dried, and powdered using domestic miller (MX-GX1521, Panasonic, Tokyo, Japan). Around 1 g of the prepared powder was added into 100 mL of boiling distilled water for 5 min and after that it was cooled to room temperature and filtered using Whatman Grade 1 filter paper. The provided C. avellana leaf extract was stored in the refrigerator (at 4°C) throughout the experiment.

According to the literature studies, 1 mM silver nitrate solution (colorless) was prepared by dissolving 0.017 g of AgNO3 in 100 mL deionized double distilled water [1,2,3]. Different amount of AgNO3 solution (15–25 mL) was mixed with different amount of C. avellana leaf extract (0.1–0.9 mL) and the mixture solutions were placed in the microwave oven (MG-2312W, LG Co., South Korea), at a constant power of 800 W and microwave heating time of 180 s.

2.3 Physico-chemical assay

The main functional groups related to the bioactive compounds of C. avellana leaf extract, which play the main role in the reduction and stabilization of the AgNPs, were determined using Fourier transform infrared (FT-IR) spectroscopy (Bruker Tensor 27 FT-IR spectrometer, Germany) with KBr pellets in the 4,000–400 cm−1 region. The formation of the AgNPs using C. avellana leaf extract was confirmed by a Jenway ultraviolet-visible (UV-Vis) spectrophotometer 6705 (United Kingdom). AgNPs due to their surface plasmon resonance (SPR) have broad emission peaks (λ max) in the wavelength ranging from 380 to 450 nm. This is responsible for the striking yellow–brown color of the synthesized AgNPs in various media [8]. UV-Vis spectroscopy measurements can also be used to evaluate the concentration of the AgNPs solution by establishing a standard curve using several serial dilute solutions of AgNPs (10–1,000 ppm). In fact, the absorbance unit at the place of broad emission peak (λ max) can be correlated to the concentration of the synthesized AgNPs in the colloidal solution. Mean particle size, polydispersity index (PDI), zeta potential values, and particle size distribution (PSD) of the fabricated AgNPs were measured using a dynamic light scattering (DLS) particle size analyzer (Nanotrac Wave, Microtrac, United States) at 25°C. For morphology analysis of the fabricated AgNPs, including size and shape, transmission electron microscopy (TEM) was used. For this reason, the aliquot of the formed AgNPs solution was put on a carbon-coated copper grid and the assay was completed using the instrument (TEM, CM120, Philips, Amsterdam, Netherlands) with an acceleration voltage of 120 kV.

2.4 Antifungal assay

The inhibition in the radial mycelia growth of C. coccodes and P. digitatum on the poured and dried plates with PDA incorporated with AgNPs was measured for 7 days at a temperature of 26 ± 2°C. In this method, an agar disk (5 mm in diameter) from a pure culture of the fungus was placed in the center of the PDA plates (90 mm in diameter) as control sample and those amended with AgNPs and leaf extract, and the plates were then incubated. Daily radial measurements of mycelia growth were taken until the fungus reached the edge of the control plates. Antifungal activity of the synthesized AgNPs was expressed as inhibition of the fungal hyphae growth (mm).

2.5 Optimization of the AgNPs synthesis conditions

As can be clearly observed in Table 1, experiments were randomly designed using central composite design (CCD), and response surface methodology (RSM) was used to evaluate the effects of the selected synthesized parameter on λ max (nm) and concentration (ppm) of the fabricated AgNPs. According to our previous studies, two AgNPs synthesis parameters, namely C. avellana leaf extract (0.10–0.90 mL, X 1) and 1 mM silver salt solution (15–25 mL, X 2), were selected to be optimized for the synthesis of AgNPs with minimum particle size, as manifested in place of λ max, and maximum concentration, based on the absorbance unit of λ max and standard equation [1,2,3]. As clearly observed in Table 1, 13 experimental treatments were assigned with five different levels for each synthesis parameter [13]. In order to model the λ max (nm) and concentration (ppm) of the synthesized AgNPs to the synthesis parameters, a second order polynomial equation was selected [14]. The suitability of the generated models was studied accounting for the coefficient of determination (R 2) [15,16]. The R 2 and the p-value of the lack of fit were used to assess the fitness and accuracy of the generated models according to the obtained experimental values. The one-way analysis of variance was also used to provide the significance determinations of the resulted models in term of p-value (<0.05) [17]. In order to obtain the optimum synthesis conditions with the desired response variables, numerical multiple response and graphical optimizations were used [17]. Three additional approval tests were performed at obtained optimum synthesis conditions to verify the validity of the statistical experimental method [18]. The Minitab software (v.16 statistical package, Minitab Inc., PA, United States) was used to design experiments, statistical analysis, and optimization.

Table 1

Experimental runs according to the CCD and response variables for AgNPs synthesis

Sample no. Amount of leaf extract (mL) Amount of silver salt (mL) λ max (nm) Concentration of AgNPs (ppm)
1 0.10 20.00 414 71
2 0.78 16.46 424 133
3 0.78 23.53 419 111
4 0.50 15.00 424 99
5 0.50 25.00 413 67
6 0.50 20.00 427 76
7 0.50 20.00 421 72
8 0.22 23.53 412 72
9 0.50 20.00 423 67
10 0.90 20.00 421 101
11 0.22 16.46 418 73
12 0.50 20.00 427 76
13 0.50 20.00 421 63

3 Results and discussion

3.1 Fabrication of AgNPs

Colloidal mixture solutions containing synthesized AgNPs indicated broad emission peaks (λ max) ranging from 380 to 450 nm due to their SPR [10]. As clearly observed in Table 1, the λ max of the synthesized AgNPs was changed from 412 to 427 nm which those values were in the mentioned λ max range for the AgNPs. This indicated that the C. avellana leaf extract successfully reduced silver ions and formed AgNPs.

Figure 1 shows the FT-IR spectrum of C. avellana leaf extract which contained four main peaks. The absorption band at 3452.90 cm−1 was related to the stretching vibrations of the hydroxyl group which is the main functional group in the chemical structure of alcohols and phenolic compounds and responsible for reducing silver ions into silver atoms [2]. The observed band at 2076.86 cm−1 was corresponded to the C═N and C═C stretching, and the band centered at 1637.92 cm−1 was related to O–H bending in carboxylic acid (COOH) groups. These functional groups were in the chemical structure of 4- and 5-caffeoylquinic acids, caffeoyltartaric acid, p-Coumaroyltartaric acid, and p-Coumaric acid, the main existed carboxylic acids, which those were found in the hazelnut leaf [10,11]. The peak centered at 675.59 cm−1 was related to the C–C ring deformation that was observed in the chemical structure of the main bioactive compounds present in the prepared hazelnut leaf extract [9]. FT-IR analysis indicated that the prepared extract had several reducing bioactive compounds with hydroxyl and carboxylic acid groups in their structures, which could effectively reduce silver ions and convert them into AgNPs [8,19].

Figure 1 The FT-IR spectrum of C. avellana leaf extract.
Figure 1

The FT-IR spectrum of C. avellana leaf extract.

3.2 Model generation

According to the obtained experimental data for the response variables (Table 1), final models were generated for λ max (Y 1) and concentration (Y 2) of the synthesized AgNPs (Eqs. 1 and 2):

(1) Y 1 = 290 – 39.5 X 1 – 20.7 X 2 + 238.3 X 1 2 + 0.5 X 2 2 – 5.4 X 1 X 2 , ( R 2 = 96.03 % )

(2) Y 2 = 347.5 + 43.7 X 1 + 7.2 X 2 – 38.5 X 1 2 – 0.2 X 2 2 + 0.2 X 1 X 2 , ( R 2 = 86.19 % )

where X i , X i 2 , and X i X j correspond to the linear, quadratic, and interaction effects of the amount of leaf extract (X 1) and amount of silver salt (X 2). Statistical analysis indicated that the linear effect of the amount of silver salt and the quadratic effects of both the selected synthesis parameters had significant (p < 0.05) effects on λ max and concentration of the synthesized AgNPs. The high values for R 2 of λ max and concentration of the synthesized AgNPs confirmed the suitability of the resulting models. Barabadi et al. also synthesized AgNPs induced by Penicillium citrinum and reported that the obtained R 2 value of 0.889 for the fitted model indicated a good correlation between the observed and predicted response values [20].

3.3 Optimization of the AgNPs synthesis parameters

The aim of the optimization for AgNPs synthesis conditions is fabrication of AgNPs with minimum particle size, which could be manifested in the small values for λ max and maximum concentration. In order to indicate the optimum area for AgNPs synthesized parameters, graphical optimization using an overlaid contour plot was plotted (Figure 2). The white colored area in Figure 2 indicates the desired amounts of C. avellana leaf extract and AgNO3 solution to synthesize AgNPs with small particle size and high concentration. A numerical multiple optimization also indicated that 0.9 mL of C. avellana leaf extract and 25 mL of AgNO3 would give the most desirable NPs with the λ max of 412 nm and concentration of 140 ppm.

Figure 2 Overlaid contour plot of AgNPs λ max and concentration with acceptable levels as a function of the amount of C. avellana leaf extract and amount of AgNO3 solution.
Figure 2

Overlaid contour plot of AgNPs λ max and concentration with acceptable levels as a function of the amount of C. avellana leaf extract and amount of AgNO3 solution.

According to the provided standard curved using several serial dilute solutions of the prepared standard AgNPs colloidal solution, the standard Eq. 3 was obtained as

(3) C = 26.75 X − 0.54

where C and X are the concentration and the absorbance units at λ max, respectively. As can be clearly observed in Figure 3, the absorbance unit of the diluted solution (one time) containing synthesized AgNPs, using obtained optimum conditions, was 2.62. The obtained results revealed that using the obtained optimum conditions, AgNPs with a concentration of 140 ppm were synthesized. Ahmadi et al. also studied green synthesized AgNPs using Aloe vera leaf extract and reported that the fabricated AgNPs using obtained optimum amount of 1 mM silver salt solution (9 mL) and microwave heating time (360 s) had λ max and concentration of 410 and 64 ppm, respectively [2].

Figure 3 UV-Vis spectra (a) and PSD (b) of the mixture solution containing synthesized AgNPs at optimum synthesis conditions.
Figure 3

UV-Vis spectra (a) and PSD (b) of the mixture solution containing synthesized AgNPs at optimum synthesis conditions.

3.4 Characteristics of the synthesized AgNPs at obtained optimum conditions

The fabrication of AgNPs was confirmed by the observation of a broad emission peak in the UV-Vis spectra of the colloidal solution containing synthesized AgNPs using obtained optimum conditions (Figure 3a). Furthermore, DLS analysis demonstrated that these NPs had mean particle size, PDI, and zeta potential values of 103.5 nm, 0.209, and −21.8 mV, respectively. The PSDs of the produced AgNPs are shown in Figure 3b. DLS analysis of the synthesized AgNPs using A. vera leaf extract and microwave heating by Ahmadi et al. indicated that the fabricated NPs had particle size and zeta potential values of 46 nm and +15.5 mV, respectively [2]. The small particle size of the AgNPs synthesized by Ahmadi et al., compared to the particle size of the fabricated AgNPs in our study, could be explained by the fact that, by increasing the microwave heating time, the collision rate of the formed NPs increased which reduced the particle size of the AgNPs [3]. The morphology of the fabricated AgNPs at optimum synthesis conditions is presented in Figure 4. As can be observed in the TEM image, the spherical shaped AgNPs were well dispersed in the colloidal solution. The spherical shape could be related to their minimum surface energy and their wide dispersion could be correlated to their high thermodynamic stability, because of the high value of the zeta potential [1,2,3,21].

Figure 4 The TEM image of the synthesized AgNPs at obtained optimum synthesis conditions using the C. avellana leaf extract.
Figure 4

The TEM image of the synthesized AgNPs at obtained optimum synthesis conditions using the C. avellana leaf extract.

3.5 Antifungal activity of the synthesized AgNPs at obtained optimum conditions

The antifungal activity of the synthesized AgNPs using C. avellana leaf extract as manifested in the inhibition of mycelia growth of C. coccodes and P. digitatum is shown in Figure 5. As can be seen in these figures, the synthesized AgNPs using C. avellana leaf extract showed high fungicidal activity against both the selected fungi strains. As can be observed in Figure 5a, after 7 days of incubation, the diameters of the mycelia growth of C. coccodes in the control plate and the one containing PDA and the synthesized AgNPs were 42 and 33 nm, respectively. On the other hand, the diameters of the mycelia growth of P. digitatum in the control plate and the one containing PDA and the synthesized AgNPs were 39 and 16 nm, respectively (Figure 5b). The obtained results revealed that the fabricated AgNPs could effectively inhibit the mycelia growth of P. digitatum compared to that of C. coccodes. In fact, the surface of AgNPs can easily form a layer of water and thus many silver ions can be released from AgNPs into the water. On the other hand, the main composition of the fungus cell membrane is phospholipid bilayers and protein molecules having negative electricity which make the whole cell membrane negatively charged. Therefore, the silver ions with positive electricity have the ability to attach to the cell membrane quickly, which alters or damages their structures [22,23,24]. The obtained results were in line with the finding of Mohammadlou et al. [1]. They found that the synthesized AgNPs using Pelargonium leaf extract, effectively inhibited the growth of Aspergillus flavus and Aspergillus terreus. Jafarizadeh-Malmiri et al. successfully used edible coating based on carboxymethyl cellulose and AgNPs to inhibit the growth of Colletotrichum musae on banana fruit and increased its shelf life [23].

Figure 5 Effect of the synthesized AgNPs on inhibiting the mycelia growth of C. coccodes (a) and P. digitatum (b). Values are mean of the three replicates.
Figure 5

Effect of the synthesized AgNPs on inhibiting the mycelia growth of C. coccodes (a) and P. digitatum (b). Values are mean of the three replicates.

4 Conclusion

The hazelnut leaf extract indicated high reduction potential to reduce silver ions and convert them into AgNPs. Microwave heating could also accelerate the fabrication rate of AgNPs to minimize energy consumption. The obtained results indicated the usefulness of RSM for studying the effects of the synthesis conditions on response variables and their optimization to synthesize spherical AgNPs with more desirable physico-chemical characteristics and high fungicidal activity. The developed intensified synthesis process to fabricate AgNPs, based on using hazelnut leaf extract, microwave heating, and obtained optimum conditions, can be used to synthesize other novel metal and metal oxide NPs. Furthermore, the provided colloidal solutions containing synthesized AgNPs and hazelnut leaf extract can be utilized in various areas that need antimicrobial agents in their products.

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Acknowledgments

This study was undertaken with material support from the Najian Herbal Group (Tabriz, Iran). The authors appreciate this support. This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

  1. Funding information: The authors appreciate the Sahand University of Technology and Najian Herbal Group (Tabriz, Iran) for their materials, analyses, and financial support.

  2. Author contributions: Mahsa Eshghi: methodology, validation, investigation; Asa Kamali-Shojaei: resources, data curation; Hamideh Vaghari: writing – original draft, visualization; Yahya Najian: data analysis, design of experiments; Zahra Mohebian: statistical analysis; Omid Ahmadi: formal analysis; Hoda Jafarizadeh-Malmiri: writing final manuscript, review and editing, supervision, project administration.

  3. Conflict of interest: Authors state no conflict of interest.

  4. Data availability statement: All data generated or analyzed during this study are included in this published article.

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Received: 2021-06-23
Revised: 2021-09-01
Accepted: 2021-09-21
Published Online: 2021-10-11

© 2021 Mahsa Eshghi et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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  28. Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus
  29. Mixed gel electrolytes: Synthesis, characterization, and gas release on PbSb electrode
  30. Supported on mesoporous silica nanospheres, molecularly imprinted polymer for selective adsorption of dichlorophen
  31. Synthesis of zeolite from fly ash and its adsorption of phosphorus in wastewater
  32. Development of a continuous PET depolymerization process as a basis for a back-to-monomer recycling method
  33. Green synthesis of ZnS nanoparticles and fabrication of ZnS–chitosan nanocomposites for the removal of Cr(vi) ion from wastewater
  34. Synthesis, surface modification, and characterization of Fe3O4@SiO2 core@shell nanostructure
  35. Antioxidant potential of bulk and nanoparticles of naringenin against cadmium-induced oxidative stress in Nile tilapia, Oreochromis niloticus
  36. Variability and improvement of optical and antimicrobial performances for CQDs/mesoporous SiO2/Ag NPs composites via in situ synthesis
  37. Green synthesis of silver nanoparticles: Characterization and its potential biomedical applications
  38. Green synthesis, characterization, and antimicrobial activity of silver nanoparticles prepared using Trigonella foenum-graecum L. leaves grown in Saudi Arabia
  39. Intensification process in thyme essential oil nanoemulsion preparation based on subcritical water as green solvent and six different emulsifiers
  40. Synthesis and biological activities of alcohol extract of black cumin seeds (Bunium persicum)-based gold nanoparticles and their catalytic applications
  41. Digera muricata (L.) Mart. mediated synthesis of antimicrobial and enzymatic inhibitory zinc oxide bionanoparticles
  42. Aqueous synthesis of Nb-modified SnO2 quantum dots for efficient photocatalytic degradation of polyethylene for in situ agricultural waste treatment
  43. Study on the effect of microwave roasting pretreatment on nickel extraction from nickel-containing residue using sulfuric acid
  44. Green nanotechnology synthesized silver nanoparticles: Characterization and testing its antibacterial activity
  45. Phyto-fabrication of selenium nanorods using extract of pomegranate rind wastes and their potentialities for inhibiting fish-borne pathogens
  46. Hydrophilic modification of PVDF membranes by in situ synthesis of nano-Ag with nano-ZrO2
  47. Paracrine study of adipose tissue-derived mesenchymal stem cells (ADMSCs) in a self-assembling nano-polypeptide hydrogel environment
  48. Study of the corrosion-inhibiting activity of the green materials of the Posidonia oceanica leaves’ ethanolic extract based on PVP in corrosive media (1 M of HCl)
  49. Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
  50. Ionic liquids as capping agents of silver nanoparticles. Part II: Antimicrobial and cytotoxic study
  51. CO2 hydrogenation to dimethyl ether over In2O3 catalysts supported on aluminosilicate halloysite nanotubes
  52. Corylus avellana leaf extract-mediated green synthesis of antifungal silver nanoparticles using microwave irradiation and assessment of their properties
  53. Novel design and combination strategy of minocycline and OECs-loaded CeO2 nanoparticles with SF for the treatment of spinal cord injury: In vitro and in vivo evaluations
  54. Fe3+ and Ce3+ modified nano-TiO2 for degradation of exhaust gas in tunnels
  55. Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
  56. Synthesis of greener silver nanoparticle-based chitosan nanocomposites and their potential antimicrobial activity against oral pathogens
  57. Baeyer–Villiger co-oxidation of cyclohexanone with Fe–Sn–O catalysts in an O2/benzaldehyde system
  58. Increased flexibility to improve the catalytic performance of carbon-based solid acid catalysts
  59. Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
  60. Green-synthesized silver nanoparticles with aqueous extract of green algae Chaetomorpha ligustica and its anticancer potential
  61. Curcumin-removed turmeric oleoresin nano-emulsion as a novel botanical fungicide to control anthracnose (Colletotrichum gloeosporioides) in litchi
  62. Antibacterial greener silver nanoparticles synthesized using Marsilea quadrifolia extract and their eco-friendly evaluation against Zika virus vector, Aedes aegypti
  63. Optimization for simultaneous removal of NH3-N and COD from coking wastewater via a three-dimensional electrode system with coal-based electrode materials by RSM method
  64. Effect of Cu doping on the optical property of green synthesised l-cystein-capped CdSe quantum dots
  65. Anticandidal potentiality of biosynthesized and decorated nanometals with fucoidan
  66. Biosynthesis of silver nanoparticles using leaves of Mentha pulegium, their characterization, and antifungal properties
  67. A study on the coordination of cyclohexanocucurbit[6]uril with copper, zinc, and magnesium ions
  68. Ultrasound-assisted l-cysteine whole-cell bioconversion by recombinant Escherichia coli with tryptophan synthase
  69. Green synthesis of silver nanoparticles using aqueous extract of Citrus sinensis peels and evaluation of their antibacterial efficacy
  70. Preparation and characterization of sodium alginate/acrylic acid composite hydrogels conjugated to silver nanoparticles as an antibiotic delivery system
  71. Synthesis of tert-amylbenzene for side-chain alkylation of cumene catalyzed by a solid superbase
  72. Punica granatum peel extracts mediated the green synthesis of gold nanoparticles and their detailed in vivo biological activities
  73. Simulation and improvement of the separation process of synthesizing vinyl acetate by acetylene gas-phase method
  74. Review Articles
  75. Carbon dots: Discovery, structure, fluorescent properties, and applications
  76. Potential applications of biogenic selenium nanoparticles in alleviating biotic and abiotic stresses in plants: A comprehensive insight on the mechanistic approach and future perspectives
  77. Review on functionalized magnetic nanoparticles for the pretreatment of organophosphorus pesticides
  78. Extraction and modification of hemicellulose from lignocellulosic biomass: A review
  79. Topical Issue: Recent advances in deep eutectic solvents: Fundamentals and applications (Guest Editors: Santiago Aparicio and Mert Atilhan)
  80. Delignification of unbleached pulp by ternary deep eutectic solvents
  81. Removal of thiophene from model oil by polyethylene glycol via forming deep eutectic solvents
  82. Valorization of birch bark using a low transition temperature mixture composed of choline chloride and lactic acid
  83. Topical Issue: Flow chemistry and microreaction technologies for circular processes (Guest Editor: Gianvito Vilé)
  84. Stille, Heck, and Sonogashira coupling and hydrogenation catalyzed by porous-silica-gel-supported palladium in batch and flow
  85. In-flow enantioselective homogeneous organic synthesis
https://doi.org/10.1515/gps-2021-0062
Keywords for this article
antifungal activity; Corylus avellana; microwave irradiation; optimization; silver nanoparticles
Creative Commons
BY 4.0

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  27. A green method for the preparation of phoxim based on high-boiling nitrite
  28. Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus
  29. Mixed gel electrolytes: Synthesis, characterization, and gas release on PbSb electrode
  30. Supported on mesoporous silica nanospheres, molecularly imprinted polymer for selective adsorption of dichlorophen
  31. Synthesis of zeolite from fly ash and its adsorption of phosphorus in wastewater
  32. Development of a continuous PET depolymerization process as a basis for a back-to-monomer recycling method
  33. Green synthesis of ZnS nanoparticles and fabrication of ZnS–chitosan nanocomposites for the removal of Cr(vi) ion from wastewater
  34. Synthesis, surface modification, and characterization of Fe3O4@SiO2 core@shell nanostructure
  35. Antioxidant potential of bulk and nanoparticles of naringenin against cadmium-induced oxidative stress in Nile tilapia, Oreochromis niloticus
  36. Variability and improvement of optical and antimicrobial performances for CQDs/mesoporous SiO2/Ag NPs composites via in situ synthesis
  37. Green synthesis of silver nanoparticles: Characterization and its potential biomedical applications
  38. Green synthesis, characterization, and antimicrobial activity of silver nanoparticles prepared using Trigonella foenum-graecum L. leaves grown in Saudi Arabia
  39. Intensification process in thyme essential oil nanoemulsion preparation based on subcritical water as green solvent and six different emulsifiers
  40. Synthesis and biological activities of alcohol extract of black cumin seeds (Bunium persicum)-based gold nanoparticles and their catalytic applications
  41. Digera muricata (L.) Mart. mediated synthesis of antimicrobial and enzymatic inhibitory zinc oxide bionanoparticles
  42. Aqueous synthesis of Nb-modified SnO2 quantum dots for efficient photocatalytic degradation of polyethylene for in situ agricultural waste treatment
  43. Study on the effect of microwave roasting pretreatment on nickel extraction from nickel-containing residue using sulfuric acid
  44. Green nanotechnology synthesized silver nanoparticles: Characterization and testing its antibacterial activity
  45. Phyto-fabrication of selenium nanorods using extract of pomegranate rind wastes and their potentialities for inhibiting fish-borne pathogens
  46. Hydrophilic modification of PVDF membranes by in situ synthesis of nano-Ag with nano-ZrO2
  47. Paracrine study of adipose tissue-derived mesenchymal stem cells (ADMSCs) in a self-assembling nano-polypeptide hydrogel environment
  48. Study of the corrosion-inhibiting activity of the green materials of the Posidonia oceanica leaves’ ethanolic extract based on PVP in corrosive media (1 M of HCl)
  49. Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
  50. Ionic liquids as capping agents of silver nanoparticles. Part II: Antimicrobial and cytotoxic study
  51. CO2 hydrogenation to dimethyl ether over In2O3 catalysts supported on aluminosilicate halloysite nanotubes
  52. Corylus avellana leaf extract-mediated green synthesis of antifungal silver nanoparticles using microwave irradiation and assessment of their properties
  53. Novel design and combination strategy of minocycline and OECs-loaded CeO2 nanoparticles with SF for the treatment of spinal cord injury: In vitro and in vivo evaluations
  54. Fe3+ and Ce3+ modified nano-TiO2 for degradation of exhaust gas in tunnels
  55. Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
  56. Synthesis of greener silver nanoparticle-based chitosan nanocomposites and their potential antimicrobial activity against oral pathogens
  57. Baeyer–Villiger co-oxidation of cyclohexanone with Fe–Sn–O catalysts in an O2/benzaldehyde system
  58. Increased flexibility to improve the catalytic performance of carbon-based solid acid catalysts
  59. Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
  60. Green-synthesized silver nanoparticles with aqueous extract of green algae Chaetomorpha ligustica and its anticancer potential
  61. Curcumin-removed turmeric oleoresin nano-emulsion as a novel botanical fungicide to control anthracnose (Colletotrichum gloeosporioides) in litchi
  62. Antibacterial greener silver nanoparticles synthesized using Marsilea quadrifolia extract and their eco-friendly evaluation against Zika virus vector, Aedes aegypti
  63. Optimization for simultaneous removal of NH3-N and COD from coking wastewater via a three-dimensional electrode system with coal-based electrode materials by RSM method
  64. Effect of Cu doping on the optical property of green synthesised l-cystein-capped CdSe quantum dots
  65. Anticandidal potentiality of biosynthesized and decorated nanometals with fucoidan
  66. Biosynthesis of silver nanoparticles using leaves of Mentha pulegium, their characterization, and antifungal properties
  67. A study on the coordination of cyclohexanocucurbit[6]uril with copper, zinc, and magnesium ions
  68. Ultrasound-assisted l-cysteine whole-cell bioconversion by recombinant Escherichia coli with tryptophan synthase
  69. Green synthesis of silver nanoparticles using aqueous extract of Citrus sinensis peels and evaluation of their antibacterial efficacy
  70. Preparation and characterization of sodium alginate/acrylic acid composite hydrogels conjugated to silver nanoparticles as an antibiotic delivery system
  71. Synthesis of tert-amylbenzene for side-chain alkylation of cumene catalyzed by a solid superbase
  72. Punica granatum peel extracts mediated the green synthesis of gold nanoparticles and their detailed in vivo biological activities
  73. Simulation and improvement of the separation process of synthesizing vinyl acetate by acetylene gas-phase method
  74. Review Articles
  75. Carbon dots: Discovery, structure, fluorescent properties, and applications
  76. Potential applications of biogenic selenium nanoparticles in alleviating biotic and abiotic stresses in plants: A comprehensive insight on the mechanistic approach and future perspectives
  77. Review on functionalized magnetic nanoparticles for the pretreatment of organophosphorus pesticides
  78. Extraction and modification of hemicellulose from lignocellulosic biomass: A review
  79. Topical Issue: Recent advances in deep eutectic solvents: Fundamentals and applications (Guest Editors: Santiago Aparicio and Mert Atilhan)
  80. Delignification of unbleached pulp by ternary deep eutectic solvents
  81. Removal of thiophene from model oil by polyethylene glycol via forming deep eutectic solvents
  82. Valorization of birch bark using a low transition temperature mixture composed of choline chloride and lactic acid
  83. Topical Issue: Flow chemistry and microreaction technologies for circular processes (Guest Editor: Gianvito Vilé)
  84. Stille, Heck, and Sonogashira coupling and hydrogenation catalyzed by porous-silica-gel-supported palladium in batch and flow
  85. In-flow enantioselective homogeneous organic synthesis
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