Startseite Effect of calcium lactate in standard diet on selected markers of oxidative stress and inflammation in ovariectomized rats
Artikel Open Access

Effect of calcium lactate in standard diet on selected markers of oxidative stress and inflammation in ovariectomized rats

  • Natalia Wawrzyniak , Anna Gramza-Michałowska , Paweł Kołodziejski und Joanna Suliburska EMAIL logo
Veröffentlicht/Copyright: 28. November 2022
Artikel downloaden (PDF)
Open Chemistry
Aus der Zeitschrift Open Chemistry Band 20 Heft 1

Abstract

The effectiveness of calcium depends on its source, i.e., salt it is supplied with. This study aimed to determine the effects of calcium lactate in diet on inflammation and oxidative stress markers in ovariectomized rats. A total of 40 female Wistar rats were included in this study, which were divided into four groups. The control group was fed a standard diet, whereas the remaining three groups were ovariectomized and provided a standard diet containing calcium carbonate (OVX), a calcium-deficient diet (OVX_DEF), or a diet containing calcium lactate (OVX_CaL). The nutritional intervention lasted for 12 weeks, and then, the rats were sacrificed. Tissue and blood samples were taken and evaluated for cyclooxygenase 1 (COX-1), cyclooxygenase 2, and thiobarbituric acid reactive substance contents in the liver and serum, and total antioxidant status and lipoxygenase 1 contents only in the serum using enzyme-linked immunosorbent assay. Differences were observed in the effects of calcium carbonate and calcium lactate on the COX-1 content in the serum of ovariectomized rats: a lower COX-1 concentration was observed in the case of the calcium lactate diet. No significant differences were observed for the other parameters.

Keywords: calcium carbonate; calcium lactate; inflammation; oxidative stress; ovariectomy

1 Introduction

Menopause in women is characterized by decreased bone mineral density and increased fragility of the skeleton, due to estrogen deficiency and negative calcium balance [1]. Calcium supplementation during menopause may improve bone mineral density. Calcium can be supplemented via sources such as hydroxyapatite, bone meal, and calcium salts. The most commonly used calcium source is calcium carbonate – inorganic salt, which is commonly found in the earth’s crust, shellfish eggs, oysters, and dark green leafy vegetables. The bioavailability of calcium carbonate is comparable to that of calcium citrate, orange juice fortified with calcium citrate malate, or skim milk. Furthermore, calcium carbonate chelates oxalate and thus prevents the formation of kidney stones [2,3]. It is the most commonly used calcium salt in nutritional intervention research [4–6] and is the chief ingredient of preparations prescribed by orthopedic surgeons and pediatricians (followed by calcium citrate) [7,8]. However, in supplements for postmenopausal women, calcium lactate is commonly used due to its ability to reduce the bone degradation rate [9].

Osteoporosis not only results in the loss of bone mineral mass but also accompanies numerous processes such as oxidative stress and inflammation. It is an inflammatory disease, in which bone resorption is associated with the formation of proinflammatory cytokines and macrophages. Activated leukocytes induce the formation of osteoclasts, and some of the known proinflammatory cytokines involved in this process are TNF-α, IL-6, and IL-1 [10]. Cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) – enzymes that lead to the formation of prostaglandins – are also involved in the regulation of inflammation. COX-1 regulates normal body responses and is expressed in most of the cells, whereas COX-2 is only expressed pathologically and is induced by TNF-α, IL-1, and lipopolysaccharide [11]. Therefore, COX-2 plays a key role in response to inflammation. COX-2-stimulated prostaglandin E2 causes inflammation in bone tissue, thus increasing the number of osteoclasts [12]. Although estrogen deficiency is considered a major factor for postmenopausal osteoporosis, oxidative stress also leads to a significant loss of bone mineral mass. An increase in reactive oxygen species (ROS) is associated with aging and regulates bone cell survival [13]. Throughout life, oxidative defense is essential for maintaining bone health, and a reduction in the level of sex hormones weakens oxidative defense and thus increases bone resorption [14]. The imbalance between the antioxidant system and the ROS level is a serious threat to the proper balance between bone structure and bone resorption. Furthermore, monitoring oxidative stress biomarkers is helpful in the diagnosis of postmenopausal osteoporosis [15]. The degree of severity of oxidative stress can be determined by monitoring the concentration of the fat peroxidation products (lipoxygenase 1 [LOX-1] and thiobarbituric acid reactive substances [TBARS]) and total antioxidant status (TAS) [15–17].

Previous studies have reported that an appropriate supplementation of calcium leads to a reduction in inflammation and oxidative stress by lowering the expression of IL-6 and TNF-α and reducing the activity of lipogenesis, as well as restoring the physiological balance between the levels of ROS and antioxidants [18]. Calcium supplementation reduces oxidative stress by increasing the concentration of antioxidant enzymes in the serum [19] and the plasma total antioxidant capacity [20]. In addition, calcium supplementation with other minerals and vitamins lowers the level of malondialdehyde – an indicator of oxidative stress [21,22].

However, whether calcium supplementation improves inflammation and antioxidant status in women under menopausal conditions and whether various calcium salts have a similar effect on the body are not known. Therefore, this study aimed to compare the effects of calcium lactate and calcium carbonate (which was used in the standard diet) on selected markers of inflammation and oxidative stress in ovariectomized rats.

2 Methods

2.1 Materials and reagents

Ingredients of the animal diets and their manufacturers were as follows: choline, cysteine, and water-soluble vitamins were purchased from Sigma-Aldrich (Darmstadt, Germany); minerals were purchased from Alfa Chem (Poznań, Poland); fat-soluble vitamins were purchased from a local pharmacy; corn starch, dextrin, casein, and cellulose were purchased from Hortimex (Konin, Poland); and sucrose and oil rapeseed were purchased from a local grocery store. Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Sun Red (Shanghai, China).

2.2 Animals

Forty 12-week-old female Wistar rats were purchased from the Wielkopolska Center for Advanced Technologies (Adam Mickiewicz University, Poznań, Poland). There were no significant differences in the body weight of the rats. The experiment was carried out in accordance with the guidelines for the care and use of laboratory animals. The animals were kept under appropriate conditions: 12 h light/dark cycle, in individual cages. Before the experiment, the animals were acclimatized for 1 week and were provided ad libitum access to deionized water and feed.

2.3 Experimental protocols

Before the introduction of the modified diets, the animals were divided into four groups of ten rats each. Ovariectomy was performed in three groups (30 rats). Throughout the experiment as well as during the adaptation and weekly recovery after ovariectomy, the rats were fed the standard diet AIN-93M. Then, a 12-week nutritional intervention was introduced. The control group (C) was fed the standard diet (standard diet contains calcium carbonate as a source of calcium). The ovariectomized groups were fed as follows: group OVX was fed the standard diet (standard diet contains calcium carbonate as a source of calcium), OVX_DEF group was fed calcium-deficient diet (obtained by not adding calcium in the standard diet), OVX_CaL was fed the diet with calcium lactate. Calcium salt was added to ensure a constant level of calcium in all diets (except in the deficient one), i.e., about 5 g/kg, which is the same as in the standard AIN-93M diet. The schematic of the experiment is illustrated in Figure 1. Body weight of the rats was measured weekly, and their food consumption was recorded daily. In addition, the rats were subjected to a body composition analysis (Bruker LF90II) as a part of the experiment. After blood sampling, serum was obtained by centrifugation for 10 min at 4°C at 1,200×g. Then, the liver was isolated, weighed, and washed with saline. The tissues were then stored at −80°C until further analyses.

Figure 1 The scheme of the study. C, control group; OVX, ovariectomized group with standard diet; OVX_DEF, calcium-deficient ovariectomized group; OVX_CaL, ovariectomized group with calcium lactate.
Figure 1

The scheme of the study. C, control group; OVX, ovariectomized group with standard diet; OVX_DEF, calcium-deficient ovariectomized group; OVX_CaL, ovariectomized group with calcium lactate.

2.4 Diet analysis

Dietary components were determined as follows: lipids – Soxhlet method (PN-EN ISO 3947: 2001; Soxtec System, Foss Tecator); proteins – Kjeldahl method (AOAC, 1995; Foss Tecator); ash – after complete burning in a furnace muffle (AOAC, 2000); carbohydrates – based on the fat, protein, ash, and water contents; and fiber – using the enzymatic gravimetric method.

2.5 Ca analysis in diets

Before evaluating the calcium content in the diet samples, 1 g of each sample was ashed in a muffle furnace at 450°C, and the samples were dissolved in 1 mol/l nitric acid (Merck, Kenilworth, NJ, USA). The samples were then diluted with deionized water and LaCl3 (0.5%), and the calcium content was determined using flame atomic absorption spectrometry (AAS-3, Carl Zeiss, Jena, Germany). The apparatus was validated with an accuracy of 92% on a reference material (Brown Bread BCR191, Sigma-Aldrich, St. Louis, MO, USA). Each of the samples was analyzed in triplicate.

2.6 Serum parameters

Concentrations of COX-1, COX-2, LOX-1, TAS, TBARS, and estradiol in the serum and concentrations of COX-1, COX-2, and TBARS in the liver were determined using ELISA kits (SunRed, Shanghai, China). Liver tissues were previously homogenized in phosphate-buffered saline by centrifugation at 7,000×g for 20 min. An infinite F50 spectrometer was used for the analysis (Tecan Group Ltd, Mannedorf, Switzerland).

2.7 Statistical analysis

The Statistica program (StatSoft, Tulsa, OK, USA) was used for statistical analysis. Normality of the distribution of variables was determined using the Shapiro–Wilk test. Tukey’s post hoc test was used to determine the differences between the groups. A p-value of <0.05 was considered statistically significant.

3 Results

The composition of diets is presented in Table 1. The highest calorific value, as well as the highest carbohydrate content, was observed in the calcium-deficient group. This is probably due to adding corn starch as an alternative to calcium carbonate. As expected, the lowest calcium content was observed in the calcium-deficient group.

Table 1

Composition of diets (mean ± standard deviation)

Components Diets
Calcium carbonate (standard diet) (C/OVX) Calcium deficient (OVX_DEF) Calcium lactate (OVX_CaL)
Caloric value (kcal/100 g) 326.37 ± 4.48 336.01 ± 0.95 321.87 ± 3.37
Carbohydrates (g/100 g) 47.92 ± 0.60 50.23 ± 0.22 44.53 ± 0.90
Fiber (g/100 g) 23.04 ± 0.60 22.86 ± 1.50 25.05 ± 1.60
Fat (g/100 g) 3.76 ± 0.41 3.74 ± 0.31 4.33 ± 0.16
Protein (g/100 g) 13.70 ± 0.21 13.93 ± 0.45 13.67 ± 0.81
Ca (mg/g) 5.63 ± 0.37 0.64 ± 0.04 5.68 ± 0.24

C, control group; OVX, ovariectomized group with standard diet; OVX_DEF, calcium-deficient ovariectomized group; OVX_CaL, ovariectomized group with calcium lactate.

Higher body weight and fat content were observed in the ovariectomized rats, with no differences between the three groups (Table 2). In addition, a significantly lower relative weight of the liver was observed in the OVX_DEF and OVX_CaL groups compared to the control group.

Table 2

Daily intake and body composition in rats (mean ± standard deviation)

Parameter Group
C OVX OVX_DEF OVX_CaL
Daily intake of diet (g) 25.08 ± 0.63 25.11 ± 1.70 26.14 ± 1.87 25.90 ± 0.55
Daily intake of calcium (mg) 141.12 ± 3.56b 141.30 ± 9.57b 16.77 ± 1.20a 147.03 ± 3.11b
Body mass (g) 325.86 ± 25.97a 421.90 ± 55.10b 441.00 ± 70.97b 428.40 ± 51.10b
Fat tissue (%) 36.95 ± 8.63a 54.43 ± 10.26b 55.73 ± 11.11b 58.59 ± 5.89b
Relative weight of liver (%) 2.82 ± 0.24b 2.49 ± 0.68ab 2.21 ± 0.17a 2.22 ± 0.14a

C, control group; OVX, ovariectomized group with standard diet; OVX_DEF, calcium-deficient ovariectomized group; OVX_CaL, ovariectomized group with calcium lactate.

a,b Significant differences between groups (p < 0.05).

In this experiment, the concentrations of selected parameters related to oxidative stress and inflammation in the serum and liver of the ovariectomized rats were measured (Table 3). The calcium-deficient diet was not found to affect the serum concentration of COX-1 and COX-2, but some changes were observed in the rats fed with different types of calcium salts. In the OVX group, the concentration of COX-1 in the serum was similar to that in the control group, whereas the addition of calcium lactate to the diet decreased the concentration of COX-1 in the serum. Calcium lactate significantly decreased the concentration of COX-1 in the serum compared to the calcium carbonate in the diet: OVX and C groups. Calcium lactate also increased the concentration of COX-2 in the serum compared to the control group.

Table 3

Levels of parameters of oxidative stress and inflammation in rats (mean ± standard deviation)

Parameter Group
C OVX OVX_DEF OVX_CaL
Serum COX-1 (ng/mL) 5.16 ± 1.28b 4.86 ± 1.11b 4.22 ± 0.58ab 3.74 ± 0.35a
COX-2 (ng/mL) 17.04 ± 3.16a 20.41 ± 0.79ab 19.19 ± 3.26ab 21.64 ± 2.22b
LOX-1 (ng/mL) 8.57 ± 1.19 9.25 ± 1.3 8.32 ± 0.94 8.95 ± 1.45
TBARS (nmol/mL) 11.91 ± 2.51 10.69 ± 2.36 10.93 ± 2.59 13.14 ± 2.13
TAS (U/mL) 10.25 ± 2.83 8.7 ± 1.77 9.03 ± 1.69 8.1 ± 1.5
Liver COX-1 (ng/g) 25.14 ± 6.03 22.5 ± 7.59 19.48 ± 4.39 24.27 ± 6.77
COX-2 (ng/g) 53.54 ± 11.23 55.27 ± 17.54 48.56 ± 13.06 44.46 ± 5.18
TBARS (nmol/g) 39.39 ± 10.01 33.37 ± 4.05 46.4 ± 12.79 44.46 ± 6.64

C, control group; OVX, ovariectomized group with standard diet; OVX_DEF, calcium-deficient ovariectomized group; OVX_CaL, ovariectomized group with calcium lactate; COX-1, cyclooxygenase 1; COX-2, cyclooxygenase 2; LOX-1, lipoxygenase 1; TBARS, thiobarbituric acid reactive substances; TAS, total antioxidant status.

a,bStatistical differences between groups (p < 0.05).

4 Discussion

The results showed that there are differences in the effects of calcium lactate and standard diet with calcium carbonate on cyclooxygenases in ovariectomized rats, which is a novel finding of this study.

Calcium lactate significantly decreased the serum COX-1 concentration in ovariectomized rats, which may indicate less inflammation in this group. Moreover, the COX-2 concentration in the liver was the lowest (but not significantly) in the calcium lactate group, which may confirm the beneficial effect of calcium lactate. Similarly, in the study by Cha et al. in rats on a high-fat diet, supplementation with organic and inorganic calcium resulted in a reduction in inflammation, which was attributable to a change in the composition of the intestinal microbiota [23]. Increases in the serum COX-2 level in rats with calcium lactate observed in this study may be a result of ovariectomy and the diet because changes were observed between the control group (without ovariectomy) and the ovariectomized group with calcium lactate. These findings need to be further explained in future experimental and clinical studies. Several authors did not observe any influence of calcium on inflammation biomarkers and did not find any difference between the calcium salts used. Amini et al. reported no significant changes in the levels of inflammatory markers (IL-6, TNF-α) after nutritional intervention with calcium carbonate [24]. In patients with colorectal cancer, the possible anti-inflammatory effects of organic and inorganic calcium salts (carbonate and citrate) were compared, but no significant changes in the levels of inflammatory markers (CRP, proinflammatory interleukins) were observed [25]. However, in rats with nonalcoholic fatty liver disease (NAFLD), calcium carbonate inhibited the synthesis of fatty acids in the liver, which reduces inflammation [26]. As reported in previous studies, ovariectomy is related to inflammation [27–29], and COX-2 is activated by factors related to inflammation [30–32]. In this study, we did not observe significant influence of ovariectomy on inflammation markers in rats (results in C and OVX were comparable). It was observed that calcium-deprived diet slightly decreased COX-1 and COX-2 levels in serum and liver in ovariectomized rats. Disorders in extracellular calcium ion levels may be involved in these results because calcium ions are implicated in the pathophysiology of immune stress and regulate COX-2 expression [33].

In this study, the calcium salts did not affect the biomarkers of oxidative stress. However, minor associations were found. Lower serum TAS values were recorded in the groups with high levels of COX-2 in the serum. However, Rabbani et al. reported that the reduction in oxidative stress is positively correlated with the concentration markers of inflammation such as COX-2, IL-8, and CCL2 proteins [34]. The lowest concentration of TAS was observed in the ovariectomized group with calcium carbonate or calcium lactate, which may indicate an increase in oxidative stress in these groups, but it may be a consequence of ovariectomy [35,36]. Yang et al. reported that calcium supplementation does not reduce oxidative stress, which was reflected in the concentration of F2 isoprostanes in the plasma [25]. Another marker of oxidative stress is the concentration of TBARS – a fat degradation product whose concentration increases with the growth of ROS [37]. In the present study, the highest serum TBARS concentration was observed in the group fed with calcium lactate, which corresponds to a high concentration of COX-2 in the serum. MacDowell et al. reported a similar increase in the COX-2 concentration with a simultaneous decrease in antioxidant properties in patients with borderline personality disorder [38].

The results obtained in this study may be related to changes caused by ovariectomy in rats. Ovariectomy increased body mass as was expected but did not impact inflammation and oxidative stress parameters. The reduction in estrogen levels is the primary factor for the increase in body weight and the adipose tissue (AT) content in postmenopausal women. During the reproductive period, estrogens interact with AT genes, reduce the central mass, and increase the subcutaneous AT content in the femoral area [39]. Normally, menopause is associated with the development of metabolic diseases, cardiovascular diseases, and type 2 diabetes. In addition, an increase in the AT content and redistribution are observed; lipids from subcutaneous AT penetrate visceral AT and are stored there. Higher body fat is associated with increased adipocyte size and insulin resistance and a higher expression of inflammatory cytokines [40]. Therefore, it can be supposed that ovariectomized rats would have increased levels of COX-2 in both serum and liver, indicating an increase in inflammation, which was not confirmed in the present study. In this study, we observed that ovariectomy and a calcium-deprived diet did not significantly affect markers of inflammation and oxidative stress. However, the source of calcium in the diet may impact cyclooxygenases level and calcium lactate may be more beneficial for COX-1 level than calcium carbonate in menopause.

Due to several limitations of this study, some of the obtained results could not be highlighted here. In this study only selected parameters of oxidative stress and inflammation were analyzed. Some indicators of oxidative stress such as superoxide dismutase and malondialdehyde and other inflammation cytokines were not analyzed because only a limited volume of serum was obtained from rats. Moreover, only two calcium salts: calcium carbonate and calcium lactate were compared in this study.

5 Conclusions

In ovariectomized rats, calcium carbonate and calcium lactate affect the serum COX-1 concentration in different ways. Calcium lactate exhibits a serum-COX-1-lowering effect in ovariectomized rats.

Abbreviations

COX-1

cyclooxygenase 1

COX-2

cyclooxygenase 2

CRP

C reactive protein

ELISA

enzyme-linked immunosorbent assay

E2

estradiol

IL-1

interleukin-1

IL-6

interleukin-6

LOX-1

lipoxygenase 1

NAFLD

nonalcoholic fatty liver disease

TAS

total antioxidant status

TBARS

thiobarbituric acid reactive substances

TNF-α

tumor necrosis factor

  1. Funding information: This study was funded by the National Science Centre (grant number: 2018/29/B/NZ9/00461).

  2. Author contributions: Conceptualization: N.W., J.S.; methodology: J.S., N.W.; software: J.S., N.W.; validation: N.W., J.S.; formal analysis: J.S.; investigation: N.W., P.K., J.S.; resources: A.G.M.; data curation: J.S., N.W.; writing – original draft preparation: N.W., J.S.; writing – review and editing: all authors; visualization: N.W., J.S.; supervision: J.S.; project administration: A.G.M.; funding acquisition: A.G.M. All authors have read and agreed to the published version of the manuscript.

  3. Conflict of interest: The authors have declared that they have no conflict of interest.

  4. Ethical approval: All experimental procedures were performed in accordance with the EU Directive 2010/63/EU for animal experiments. Approval for the study was obtained from the Local Ethics Committee in Poznań (no. 34/2019). The reporting in the manuscript follows the recommendations in the ARRIVE guidelines.

  5. Data availability statement: The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

References

[1] Armas LAG, Recker RR. Pathophysiology of osteoporosis. New mechanistic insights. Endocrinol Metab Clin North Am. 2012;41:475–86. 10.1016/j.ecl.2012.04.006.Suche in Google Scholar PubMed

[2] Straub DA. Calcium supplementation in clinical practice: a review of forms, doses, and indications. Nutr Clin Pract Publ Am Soc Parenter Enter Nutr. 2007;22:286–96. 10.1177/0115426507022003286.Suche in Google Scholar PubMed

[3] Fritz K, Taylor K, Parmar M. Calcium carbonate. Treasure Island (FL): StatPearls Publishing; 2022.Suche in Google Scholar

[4] Downey D, Spencer SJ, Deghenghi R, Jaffe RB. Oral, water-soluble combined estrogen/calcium preparation for postmenopausal therapy. Maturitas. 2001;38:205–10. 10.1016/s0378-5122(00)00218-8.Suche in Google Scholar PubMed

[5] Moyer-Mileur LJ, Xie B, Ball SD, Pratt T. Bone mass and density response to a 12-month trial of calcium and vitamin D supplement in preadolescent girls. J Musculoskelet Neuronal Interact. 2003;3:63–70.Suche in Google Scholar

[6] Bonjour JP. Calcium and phosphate: a duet of ions playing for bone health. J Am Coll Nutr. 2011;30:438S–48S. 10.1080/07315724.2011.10719988.Suche in Google Scholar PubMed

[7] Sheth B, Akil Prabhakar S, Pawar P, Ganwir H, Panchal S, Jain A. Calcium prescription by Indian orthopaedic surgeons: a survey and a review of literature. J Clin Orthop Trauma. 2021;16:292–8. 10.1016/j.jcot.2021.02.015.Suche in Google Scholar PubMed PubMed Central

[8] Cheung MS. Drugs used in paediatric bone and calcium disorders. Endocr Dev. 2015;28:277–90. 10.1159/000381053.Suche in Google Scholar PubMed

[9] Wagner G, Kindrick S, Hertzler S, DiSilvestro RA. Effects of various forms of calcium on body weight and bone turnover markers in women participating in a weight loss program. J Am Coll Nutr. 2007;26:456–61. 10.1080/07315724.2007.10719636.Suche in Google Scholar PubMed

[10] Lacativa PGS, de Farias MLF. Osteoporosis and inflammation. Arq Bras Endocrinol Metab. 2010;54:123–32. 10.1590/s0004-27302010000200007.Suche in Google Scholar PubMed

[11] Eliopoulos AG, Dumitru CD, Wang CC, Cho J, Tsichlis PN. Induction of COX-2 by LPS in macrophages is regulated by Tpl2-dependent CREB activation signals. EMBO J. 2002;21:4831–40. 10.1093/emboj/cdf478.Suche in Google Scholar PubMed PubMed Central

[12] Zhu C, Ji Y, Liu S, Bian Z. Follicle-stimulating hormone enhances alveolar bone resorption via upregulation of cyclooxygenase-2. Am J Transl Res. 2016;8:3861–71.Suche in Google Scholar

[13] Kimball JS, Johnson JP, Carlson DA. Oxidative stress and osteoporosis. J Bone Jt Surg Am. 2021;103:1451–61. 10.2106/JBJS.20.00989.Suche in Google Scholar PubMed

[14] Manolagas SC. From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. Endocr Rev. 2010;31:266–300. 10.1210/er.2009-0024.Suche in Google Scholar PubMed PubMed Central

[15] Zhao F, Guo L, Wang X, Zhang Y. Correlation of oxidative stress-related biomarkers with postmenopausal osteoporosis: a systematic review and meta-analysis. Arch Osteoporos. 2021;16:4. 10.1007/s11657-020-00854-w.Suche in Google Scholar PubMed

[16] Altindag O, Erel O, Soran N, Celik H, Selek S. Total oxidative/anti-oxidative status and relation to bone mineral density in osteoporosis. Rheumatol Int. 2008;28:317–21. 10.1007/s00296-007-0452-0.Suche in Google Scholar PubMed

[17] Zhou Q, Zhu L, Zhang D, Li N, Li Q, Dai P, et al. Oxidative stress-related biomarkers in postmenopausal osteoporosis: a systematic review and meta-analyses. Dis Markers. 2016;2016:12. 10.1155/2016/7067984.Suche in Google Scholar PubMed PubMed Central

[18] Das S, Choudhuri D. Role of dietary calcium and its possible mechanism against metabolic disorders: a concise review. J Food Biochem. 2021;45:1–10. 10.1111/jfbc.13697.Suche in Google Scholar PubMed

[19] Gottipolu RR, Davuljigari CB. Perinatal exposure to lead: reduction in alterations of brain mitochondrial antioxidant system with calcium supplement. Biol Trace Elem Res. 2014;162:270–7. 10.1007/s12011-014-0112-7.Suche in Google Scholar PubMed

[20] Maktabi M, Jamilian M, Asemi Z. Magnesium-zinc-calcium-vitamin D Co-supplementation improves hormonal profiles, biomarkers of inflammation and oxidative stress in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Biol Trace Elem Res. 2018;182:21–8. 10.1007/s12011-017-1085-0.Suche in Google Scholar PubMed

[21] Jamilian M, Mirhosseini N, Eslahi M, Bahmani F, Shokrpour M, Chamani M, et al. The effects of magnesium–zinc–calcium–vitamin D co-supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes. BMC Pregnancy Childbirth. 2019;19:107. 10.1186/s12884-019-2258-y.Suche in Google Scholar PubMed PubMed Central

[22] Asemi Z, Karamali M, Esmaillzadeh A. Effects of calcium–vitamin D co-supplementation on glycaemic control, inflammation and oxidative stress in gestational diabetes: a randomised placebo-controlled trial. Diabetologia. 2014;57:1798–806. 10.1007/s00125-014-3293-x.Suche in Google Scholar PubMed

[23] Cha KH, Yang J-S, Kim K-A, Yoon K-Y, Song D-G, Erdene-Ochir E, et al. Improvement in host metabolic homeostasis and alteration in gut microbiota in mice on the high-fat diet: a comparison of calcium supplements. Food Res Int. 2020;136:109495. 10.1016/j.foodres.2020.109495.Suche in Google Scholar PubMed

[24] Amini S, Amani R, Jafarirad S, Cheraghian B, Sayyah M, Hemmati AA. The effect of vitamin D and calcium supplementation on inflammatory biomarkers, estradiol levels and severity of symptoms in women with postpartum depression: a randomized double-blind clinical trial. Nutr Neurosci. 2022;25:22–32. 10.1080/1028415X.2019.1707396.Suche in Google Scholar PubMed

[25] Yang B, Gross MD, Fedirko V, McCullough ML, Bostick RM. Effects of calcium supplementation on biomarkers of inflammation and oxidative stress in colorectal adenoma patients: a randomized controlled trial. Cancer Prev Res. 2015;8:1069–75. 10.1158/1940-6207.CAPR-15-0168.Suche in Google Scholar PubMed

[26] Sandeep D, Dipayan C. Calcium supplementation shows a hepatoprotective effect against high-fat diet by regulating oxidative-induced inflammatory response and lipogenesis activity in male rats. J Tradit Complement Med. 2020;10:511–9. 10.1016/j.jtcme.2019.06.002.Suche in Google Scholar PubMed PubMed Central

[27] Cline-Smith A, Axelbaum A, Shashkova E, Chakraborty M, Sanford J, Panesar P, et al. Ovariectomy activates chronic low-grade inflammation mediated by memory T cells, which promotes osteoporosis in mice. J Bone Min Res J Am Soc Bone Min Res. 2020;35:1174–87. 10.1002/jbmr.3966.Suche in Google Scholar PubMed PubMed Central

[28] Georgieva A, Eftimov M, Todorova M, Kuzmanova V, Kuzmanov A, Kuzmanov K, et al. Effects of ovariectomy-induced estrogen deficit on rat behaviour, lipid metabolism, inflammation, bone mineral density, and turnover. Folia Med (Plovdiv). 2021;63:385–91. 10.3897/folmed.63.e55267.Suche in Google Scholar PubMed

[29] Zhang Q, Song X, Chen X, Jiang R, Peng K, Tang X, et al. Antiosteoporotic effect of hesperidin against ovariectomy-induced osteoporosis in rats via reduction of oxidative stress and inflammation. J Biochem Mol Toxicol. 2021;35:e22832. 10.1002/jbt.22832.Suche in Google Scholar PubMed

[30] Yu T, Lao X, Zheng H. Influencing COX-2 activity by COX related pathways in inflammation and cancer. Mini Rev Med Chem. 2016;16:1230–43. 10.2174/1389557516666160505115743.Suche in Google Scholar PubMed

[31] Cui J, Jia J. Natural COX-2 inhibitors as promising anti-inflammatory agents: an update. Curr Med Chem. 2021;28:3622–46. 10.2174/0929867327999200917150939.Suche in Google Scholar PubMed

[32] Golden J, Illingworth L, Kavarian P, Escobar O, Delaplain P, Isani M, et al. EP2 receptor blockade attenuates COX-2 upregulation during intestinal inflammation. Shock. 2020;54:394–401. 10.1097/SHK.0000000000001444.Suche in Google Scholar PubMed PubMed Central

[33] Ogata S, Kubota Y, Satoh S, Ito S, Takeuchi H, Ashizuka M, et al. Ca2+ stimulates COX-2 expression through calcium-sensing receptor in fibroblasts. Biochem Biophys Res Commun. 2006;351:808–14. 10.1016/j.bbrc.2006.10.098.Suche in Google Scholar PubMed

[34] Rabbani N, Xue M, Weickert MO, Thornalley PJ. Reversal of insulin resistance in overweight and obese subjects by trans-resveratrol and hesperetin combination-link to dysglycemia, blood pressure, dyslipidemia, and low-grade inflammation. Nutrients. 2021;13:2374. 10.3390/nu13072374.Suche in Google Scholar PubMed PubMed Central

[35] Lee JY, Kim MC. Comparison of oxidative stress status in dogs undergoing laparoscopic and open ovariectomy. J Vet Med Sci. 2014;76:273–6. 10.1292/jvms.13-0062.Suche in Google Scholar PubMed PubMed Central

[36] Agacayak E, Basaranoglu S, Tunc SY, Icen MS, Findik FM, Kaplan I, et al. Oxidant/antioxidant status, paraoxonase activity, and lipid profile in plasma of ovariectomized rats under the influence of estrogen, estrogen combined with progesterone, and genistein. Drug Des Devel Ther. 2015;9:2975–82. 10.2147/DDDT.S82263.Suche in Google Scholar PubMed PubMed Central

[37] Czerska M, Mikołajewska K, Zieliński M, Gromadzińska J, Wąsowicz W. Today’s oxidative stress markers. Med Pr. 2015;66:393–405. 10.13075/mp.5893.00137.Suche in Google Scholar PubMed

[38] MacDowell KS, Marsá MD, Buenache E, Villatoro JML, Moreno B, Leza JC, et al. Inflammatory and antioxidant pathway dysfunction in borderline personality disorder. Psychiatry Res. 2020;284:112782. 10.1016/j.psychres.2020.112782.Suche in Google Scholar PubMed

[39] Leeners B, Geary N, Tobler PN, Asarian L. Ovarian hormones and obesity. Hum Reprod Update. 2017;23:300–21. 10.1093/humupd/dmw045.Suche in Google Scholar PubMed PubMed Central

[40] Abildgaard J, Ploug T, Al-Saoudi E, Wagner T, Thomsen C, Ewertsen C, et al. Changes in abdominal subcutaneous adipose tissue phenotype following menopause is associated with increased visceral fat mass. Sci Rep. 2021;11:14750. 10.1038/s41598-021-94189-2.Suche in Google Scholar PubMed PubMed Central

Received: 2022-09-19
Revised: 2022-10-24
Accepted: 2022-10-25
Published Online: 2022-11-28

© 2022 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Artikel in diesem Heft

  1. Regular Articles
  2. Photocatalytic degradation of Rhodamine B in aqueous phase by bimetallic metal-organic framework M/Fe-MOF (M = Co, Cu, and Mg)
  3. Assessment of using electronic portal imaging device for analysing bolus material utilised in radiation therapy
  4. A detailed investigation on highly dense CuZr bulk metallic glasses for shielding purposes
  5. Simulation of gamma-ray shielding properties for materials of medical interest
  6. Environmental impact assesment regulation applications and their analysis in Turkey
  7. Sample age effect on parameters of dynamic nuclear polarization in certain difluorobenzen isomers/MC800 asphaltene suspensions
  8. Passenger demand forecasting for railway systems
  9. Design of a Robust sliding mode controller for bioreactor cultures in overflow metabolism via an interdisciplinary approach
  10. Gamma, neutron, and heavy charged ion shielding properties of Er3+-doped and Sm3+-doped zinc borate glasses
  11. Bridging chiral de-tert-butylcalix[4]arenes: Optical resolution based on column chromatography and structural characterization
  12. Petrology and geochemistry of multiphase post-granitic dikes: A case study from the Gabal Serbal area, Southwestern Sinai, Egypt
  13. Comparison of the yield and purity of plasma exosomes extracted by ultracentrifugation, precipitation, and membrane-based approaches
  14. Bioactive triterpenoids from Indonesian medicinal plant Syzygium aqueum
  15. Investigation of the effects of machining parameters on surface integrity in micromachining
  16. The mesoporous aluminosilicate application as support for bifunctional catalysts for n-hexadecane hydroconversion
  17. Gamma-ray shielding properties of Nd2O3-added iron–boron–phosphate-based composites
  18. Numerical investigation on perforated sheet metals under tension loading
  19. Statistical analysis on the radiological assessment and geochemical studies of granite rocks in the north of Um Taghir area, Eastern Desert, Egypt
  20. Two new polypodane-type bicyclic triterpenoids from mastic
  21. Structural, physical, and mechanical properties of the TiO2 added hydroxyapatite composites
  22. Tribological properties and characterization of borided Co–Mg alloys
  23. Studies on Anemone nemorosa L. extracts; polyphenols profile, antioxidant activity, and effects on Caco-2 cells by in vitro and in silico studies
  24. Mechanical properties, elastic moduli, transmission factors, and gamma-ray-shielding performances of Bi2O3–P2O5–B2O3–V2O5 quaternary glass system
  25. Cyclic connectivity index of bipolar fuzzy incidence graph
  26. The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos
  27. Mechanical property evaluation of tellurite–germanate glasses and comparison of their radiation-shielding characteristics using EPICS2017 to other glass systems
  28. Molecular screening of ionic liquids for CO2 absorption and molecular dynamic simulation
  29. Microwave-assisted preparation of Ag/Fe magnetic biochar from clivia leaves for adsorbing daptomycin antibiotics
  30. Iminodisuccinic acid enhances antioxidant and mineral element accumulation in young leaves of Ziziphus jujuba
  31. Cytotoxic activity of guaiane-type sesquiterpene lactone (deoxycynaropicrin) isolated from the leaves of Centaurothamnus maximus
  32. Effects of welding parameters on the angular distortion of welded steel plates
  33. Simulation of a reactor considering the Stamicarbon, Snamprogetti, and Toyo patents for obtaining urea
  34. Effect of different ramie (Boehmeria nivea L. Gaud) cultivars on the adsorption of heavy metal ions cadmium and lead in the remediation of contaminated farmland soils
  35. Impact of a live bacterial-based direct-fed microbial (DFM) postpartum and weaning system on performance, mortality, and health of Najdi lambs
  36. Anti-tumor effect of liposomes containing extracted Murrayafoline A against liver cancer cells in 2D and 3D cultured models
  37. Physicochemical properties and some mineral concentration of milk samples from different animals and altitudes
  38. Copper(ii) complexes supported by modified azo-based ligands: Nucleic acid binding and molecular docking studies
  39. Diagnostic and therapeutic radioisotopes in nuclear medicine: Determination of gamma-ray transmission factors and safety competencies of high-dense and transparent glassy shields
  40. Calculation of NaI(Tl) detector efficiency using 226Ra, 232Th, and 40K radioisotopes: Three-phase Monte Carlo simulation study
  41. Isolation and identification of unstable components from Caesalpinia sappan by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography
  42. Quantification of biomarkers and evaluation of antioxidant, anti-inflammatory, and cytotoxicity properties of Dodonaea viscosa grown in Saudi Arabia using HPTLC technique
  43. Characterization of the elastic modulus of ceramic–metal composites with physical and mechanical properties by ultrasonic technique
  44. GC-MS analysis of Vespa velutina auraria Smith and its anti-inflammatory and antioxidant activities in vitro
  45. Texturing of nanocoatings for surface acoustic wave-based sensors for volatile organic compounds
  46. Insights into the molecular basis of some chalcone analogues as potential inhibitors of Leishmania donovani: An integrated in silico and in vitro study
  47. (1R,2S,5R)-5-Methyl-2-(propan-2-yl)cyclohexyl 4-amino-3-phenylbutanoate hydrochloride: Synthesis and anticonvulsant activity
  48. On the relative extraction rates of colour compounds and caffeine during brewing, an investigation of tea over time and temperature
  49. Characterization of egg shell powder-doped ceramic–metal composites
  50. Rapeseed oil-based hippurate amide nanocomposite coating material for anticorrosive and antibacterial applications
  51. Chemically modified Teucrium polium (Lamiaceae) plant act as an effective adsorbent tool for potassium permanganate (KMnO4) in wastewater remediation
  52. Efficiency analysis of photovoltaic systems installed in different geographical locations
  53. Risk prioritization model driven by success factor in the light of multicriteria decision making
  54. Theoretical investigations on the excited-state intramolecular proton transfer in the solvated 2-hydroxy-1-naphthaldehyde carbohydrazone
  55. Mechanical and gamma-ray shielding examinations of Bi2O3–PbO–CdO–B2O3 glass system
  56. Machine learning-based forecasting of potability of drinking water through adaptive boosting model
  57. The potential effect of the Rumex vesicarius water seeds extract treatment on mice before and during pregnancy on the serum enzymes and the histology of kidney and liver
  58. Impact of benzimidazole functional groups on the n-doping properties of benzimidazole derivatives
  59. Extraction of red pigment from Chinese jujube peel and the antioxidant activity of the pigment extracts
  60. Flexural strength and thermal properties of carbon black nanoparticle reinforced epoxy composites obtained from waste tires
  61. A focusing study on radioprotective and antioxidant effects of Annona muricata leaf extract in the circulation and liver tissue: Clinical and experimental studies
  62. Clinical comprehensive and experimental assessment of the radioprotective effect of Annona muricata leaf extract to prevent cellular damage in the ileum tissue
  63. Effect of WC content on ultrasonic properties, thermal and electrical conductivity of WC–Co–Ni–Cr composites
  64. Influence of various class cleaning agents for prosthesis on Co–Cr alloy surface
  65. The synthesis of nanocellulose-based nanocomposites for the effective removal of hexavalent chromium ions from aqueous solution
  66. Study on the influence of physical interlayers on the remaining oil production under different development modes
  67. Optimized linear regression control of DC motor under various disturbances
  68. Influence of different sample preparation strategies on hypothesis-driven shotgun proteomic analysis of human saliva
  69. Determination of flow distance of the fluid metal due to fluidity in ductile iron casting by artificial neural networks approach
  70. Investigation of mechanical activation effect on high-volume natural pozzolanic cements
  71. In vitro: Anti-coccidia activity of Calotropis procera leaf extract on Eimeria papillata oocysts sporulation and sporozoite
  72. Determination of oil composition of cowpea (Vigna unguiculata L.) seeds under influence of organic fertilizer forms
  73. Activated partial thromboplastin time maybe associated with the prognosis of papillary thyroid carcinoma
  74. Treatment of rat brain ischemia model by NSCs-polymer scaffold transplantation
  75. Lead and cadmium removal with native yeast from coastal wetlands
  76. Characterization of electroless Ni-coated Fe–Co composite using powder metallurgy
  77. Ferrate synthesis using NaOCl and its application for dye removal
  78. Antioxidant, antidiabetic, and anticholinesterase potential of Chenopodium murale L. extracts using in vitro and in vivo approaches
  79. Study on essential oil, antioxidant activity, anti-human prostate cancer effects, and induction of apoptosis by Equisetum arvense
  80. Experimental study on turning machine with permanent magnetic cutting tool
  81. Numerical simulation and mathematical modeling of the casting process for pearlitic spheroidal graphite cast iron
  82. Design, synthesis, and cytotoxicity evaluation of novel thiophene, pyrimidine, pyridazine, and pyridine: Griseofulvin heterocyclic extension derivatives
  83. Isolation and identification of promising antibiotic-producing bacteria
  84. Ultrasonic-induced reversible blood–brain barrier opening: Safety evaluation into the cellular level
  85. Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan
  86. Effect of calcium lactate in standard diet on selected markers of oxidative stress and inflammation in ovariectomized rats
  87. Identification of crucial salivary proteins/genes and pathways involved in pathogenesis of temporomandibular disorders
  88. Zirconium-modified attapulgite was used for removing of Cr(vi) in aqueous solution
  89. The stress distribution of different types of restorative materials in primary molar
  90. Reducing surface heat loss in steam boilers
  91. Deformation behavior and formability of friction stir processed DP600 steel
  92. Synthesis and characterization of bismuth oxide/commercial activated carbon composite for battery anode
  93. Phytochemical analysis of Ziziphus jujube leaf at different foliar ages based on widely targeted metabolomics
  94. Effects of in ovo injection of black cumin (Nigella sativa) extract on hatching performance of broiler eggs
  95. Separation and evaluation of potential antioxidant, analgesic, and anti-inflammatory activities of limonene-rich essential oils from Citrus sinensis (L.)
  96. Bioactivity of a polyhydroxy gorgostane steroid from Xenia umbellata
  97. BiCAM-based automated scoring system for digital logic circuit diagrams
  98. Analysis of standard systems with solar monitoring systems
  99. Structural and spectroscopic properties of voriconazole and fluconazole – Experimental and theoretical studies
  100. New plant resistance inducers based on polyamines
  101. Experimental investigation of single-lap bolted and bolted/bonded (hybrid) joints of polymeric plates
  102. Investigation of inlet air pressure and evaporative cooling of four different cogeneration cycles
  103. Review Articles
  104. Comprehensive review on synthesis, physicochemical properties, and application of activated carbon from the Arecaceae plants for enhanced wastewater treatment
  105. Research progress on speciation analysis of arsenic in traditional Chinese medicine
  106. Recent modified air-assisted liquid–liquid microextraction applications for medicines and organic compounds in various samples: A review
  107. An insight on Vietnamese bio-waste materials as activated carbon precursors for multiple applications in environmental protection
  108. Antimicrobial activities of the extracts and secondary metabolites from Clausena genus – A review
  109. Bioremediation of organic/heavy metal contaminants by mixed cultures of microorganisms: A review
  110. Sonodynamic therapy for breast cancer: A literature review
  111. Recent progress of amino acid transporters as a novel antitumor target
  112. Aconitum coreanum Rapaics: Botany, traditional uses, phytochemistry, pharmacology, and toxicology
  113. Corrigendum
  114. Corrigendum to “Petrology and geochemistry of multiphase post-granitic dikes: A case study from the Gabal Serbal area, Southwestern Sinai, Egypt”
  115. Corrigendum to “Design of a Robust sliding mode controller for bioreactor cultures in overflow metabolism via an interdisciplinary approach”
  116. Corrigendum to “Statistical analysis on the radiological assessment and geochemical studies of granite rocks in the north of Um Taghir area, Eastern Desert, Egypt”
  117. Corrigendum to “Aroma components of tobacco powder from different producing areas based on gas chromatography ion mobility spectrometry”
  118. Corrigendum to “Mechanical properties, elastic moduli, transmission factors, and gamma-ray-shielding performances of Bi2O3–P2O5–B2O3–V2O5 quaternary glass system”
  119. Erratum
  120. Erratum to “Copper(ii) complexes supported by modified azo-based ligands: Nucleic acid binding and molecular docking studies”
  121. Special Issue on Applied Biochemistry and Biotechnology (ABB 2021)
  122. Study of solidification and stabilization of heavy metals by passivators in heavy metal-contaminated soil
  123. Human health risk assessment and distribution of VOCs in a chemical site, Weinan, China
  124. Preparation and characterization of Sparassis latifolia β-glucan microcapsules
  125. Special Issue on the Conference of Energy, Fuels, Environment 2020
  126. Improving the thermal performance of existing buildings in light of the requirements of the EU directive 2010/31/EU in Poland
  127. Special Issue on Ethnobotanical, Phytochemical and Biological Investigation of Medicinal Plants
  128. Study of plant resources with ethnomedicinal relevance from district Bagh, Azad Jammu and Kashmir, Pakistan
  129. Studies on the chemical composition of plants used in traditional medicine in Congo
  130. Special Issue on Applied Chemistry in Agriculture and Food Science
  131. Strip spraying technology for precise herbicide application in carrot fields
  132. Special Issue on Pharmacology and Metabolomics of Ethnobotanical and Herbal Medicine
  133. Phytochemical profiling, antibacterial and antioxidant properties of Crocus sativus flower: A comparison between tepals and stigmas
  134. Antioxidant and antimicrobial properties of polyphenolics from Withania adpressa (Coss.) Batt. against selected drug-resistant bacterial strains
  135. Integrating network pharmacology and molecular docking to explore the potential mechanism of Xinguan No. 3 in the treatment of COVID-19
  136. Chemical composition and in vitro and in vivo biological assortment of fixed oil extracted from Ficus benghalensis L.
  137. A review of the pharmacological activities and protective effects of Inonotus obliquus triterpenoids in kidney diseases
  138. Ethnopharmacological study of medicinal plants in Kastamonu province (Türkiye)
  139. Protective effects of asperuloside against cyclophosphamide-induced urotoxicity and hematotoxicity in rats
  140. Special Issue on Essential Oil, Extraction, Phytochemistry, Advances, and Application
  141. Identification of volatile compounds and antioxidant, antibacterial, and antifungal properties against drug-resistant microbes of essential oils from the leaves of Mentha rotundifolia var. apodysa Briq. (Lamiaceae)
  142. Phenolic contents, anticancer, antioxidant, and antimicrobial capacities of MeOH extract from the aerial parts of Trema orientalis plant
  143. Chemical composition and antimicrobial activity of essential oils from Mentha pulegium and Rosmarinus officinalis against multidrug-resistant microbes and their acute toxicity study
  144. Special Issue on Marine Environmental Sciences and Significance of the Multidisciplinary Approaches
  145. An insightful overview of the distribution pattern of polycyclic aromatic hydrocarbon in the marine sediments of the Red Sea
  146. Antifungal–antiproliferative norcycloartane-type triterpenes from the Red Sea green alga Tydemania expeditionis
  147. Solvent effect, dipole moment, and DFT studies of multi donor–acceptor type pyridine derivative
  148. An extensive assessment on the distribution pattern of organic contaminants in the aerosols samples in the Middle East
  149. Special Issue on 4th IC3PE
  150. Energetics of carboxylic acid–pyridine heterosynthon revisited: A computational study of intermolecular hydrogen bond domination on phenylacetic acid–nicotinamide cocrystals
  151. A review: Silver–zinc oxide nanoparticles – organoclay-reinforced chitosan bionanocomposites for food packaging
  152. Green synthesis of magnetic activated carbon from peanut shells functionalized with TiO2 photocatalyst for Batik liquid waste treatment
  153. Coagulation activity of liquid extraction of Leucaena leucocephala and Sesbania grandiflora on the removal of turbidity
  154. Hydrocracking optimization of palm oil over NiMoO4/activated carbon catalyst to produce biogasoline and kerosine
  155. Special Issue on Pharmacology and metabolomics of ethnobotanical and herbal medicine
  156. Cynarin inhibits PDGF-BB-induced proliferation and activation in hepatic stellate cells through PPARγ
  157. Special Issue on The 1st Malaysia International Conference on Nanotechnology & Catalysis (MICNC2021)
  158. Surfactant evaluation for enhanced oil recovery: Phase behavior and interfacial tension
  159. Topical Issue on phytochemicals, biological and toxicological analysis of aromatic medicinal plants
  160. Phytochemical analysis of leaves and stems of Physalis alkekengi L. (Solanaceae)
  161. Phytochemical and pharmacological profiling of Trewia nudiflora Linn. leaf extract deciphers therapeutic potentials against thrombosis, arthritis, helminths, and insects
  162. Pergularia tomentosa coupled with selenium nanoparticles salvaged lead acetate-induced redox imbalance, inflammation, apoptosis, and disruption of neurotransmission in rats’ brain
  163. Protective effect of Allium atroviolaceum-synthesized SeNPs on aluminum-induced brain damage in mice
  164. Mechanism study of Cordyceps sinensis alleviates renal ischemia–reperfusion injury
  165. Plant-derived bisbenzylisoquinoline alkaloid tetrandrine prevents human podocyte injury by regulating the miR-150-5p/NPHS1 axis
  166. Network pharmacology combined with molecular docking to explore the anti-osteoporosis mechanisms of β-ecdysone derived from medicinal plants
  167. Chinese medicinal plant Polygonum cuspidatum ameliorates silicosis via suppressing the Wnt/β-catenin pathway
  168. Special Issue on Advanced Nanomaterials for Energy, Environmental and Biological Applications - Part I
  169. Investigation of improved optical and conductivity properties of poly(methyl methacrylate)–MXenes (PMMA–MXenes) nanocomposite thin films for optoelectronic applications
  170. Special Issue on Applied Biochemistry and Biotechnology (ABB 2022)
  171. Model predictive control for precision irrigation of a Quinoa crop
https://doi.org/10.1515/chem-2022-0236
Schlagwörter für diesen Artikel
calcium carbonate; calcium lactate; inflammation; oxidative stress; ovariectomy
Creative Commons
BY 4.0

Artikel in diesem Heft

  1. Regular Articles
  2. Photocatalytic degradation of Rhodamine B in aqueous phase by bimetallic metal-organic framework M/Fe-MOF (M = Co, Cu, and Mg)
  3. Assessment of using electronic portal imaging device for analysing bolus material utilised in radiation therapy
  4. A detailed investigation on highly dense CuZr bulk metallic glasses for shielding purposes
  5. Simulation of gamma-ray shielding properties for materials of medical interest
  6. Environmental impact assesment regulation applications and their analysis in Turkey
  7. Sample age effect on parameters of dynamic nuclear polarization in certain difluorobenzen isomers/MC800 asphaltene suspensions
  8. Passenger demand forecasting for railway systems
  9. Design of a Robust sliding mode controller for bioreactor cultures in overflow metabolism via an interdisciplinary approach
  10. Gamma, neutron, and heavy charged ion shielding properties of Er3+-doped and Sm3+-doped zinc borate glasses
  11. Bridging chiral de-tert-butylcalix[4]arenes: Optical resolution based on column chromatography and structural characterization
  12. Petrology and geochemistry of multiphase post-granitic dikes: A case study from the Gabal Serbal area, Southwestern Sinai, Egypt
  13. Comparison of the yield and purity of plasma exosomes extracted by ultracentrifugation, precipitation, and membrane-based approaches
  14. Bioactive triterpenoids from Indonesian medicinal plant Syzygium aqueum
  15. Investigation of the effects of machining parameters on surface integrity in micromachining
  16. The mesoporous aluminosilicate application as support for bifunctional catalysts for n-hexadecane hydroconversion
  17. Gamma-ray shielding properties of Nd2O3-added iron–boron–phosphate-based composites
  18. Numerical investigation on perforated sheet metals under tension loading
  19. Statistical analysis on the radiological assessment and geochemical studies of granite rocks in the north of Um Taghir area, Eastern Desert, Egypt
  20. Two new polypodane-type bicyclic triterpenoids from mastic
  21. Structural, physical, and mechanical properties of the TiO2 added hydroxyapatite composites
  22. Tribological properties and characterization of borided Co–Mg alloys
  23. Studies on Anemone nemorosa L. extracts; polyphenols profile, antioxidant activity, and effects on Caco-2 cells by in vitro and in silico studies
  24. Mechanical properties, elastic moduli, transmission factors, and gamma-ray-shielding performances of Bi2O3–P2O5–B2O3–V2O5 quaternary glass system
  25. Cyclic connectivity index of bipolar fuzzy incidence graph
  26. The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos
  27. Mechanical property evaluation of tellurite–germanate glasses and comparison of their radiation-shielding characteristics using EPICS2017 to other glass systems
  28. Molecular screening of ionic liquids for CO2 absorption and molecular dynamic simulation
  29. Microwave-assisted preparation of Ag/Fe magnetic biochar from clivia leaves for adsorbing daptomycin antibiotics
  30. Iminodisuccinic acid enhances antioxidant and mineral element accumulation in young leaves of Ziziphus jujuba
  31. Cytotoxic activity of guaiane-type sesquiterpene lactone (deoxycynaropicrin) isolated from the leaves of Centaurothamnus maximus
  32. Effects of welding parameters on the angular distortion of welded steel plates
  33. Simulation of a reactor considering the Stamicarbon, Snamprogetti, and Toyo patents for obtaining urea
  34. Effect of different ramie (Boehmeria nivea L. Gaud) cultivars on the adsorption of heavy metal ions cadmium and lead in the remediation of contaminated farmland soils
  35. Impact of a live bacterial-based direct-fed microbial (DFM) postpartum and weaning system on performance, mortality, and health of Najdi lambs
  36. Anti-tumor effect of liposomes containing extracted Murrayafoline A against liver cancer cells in 2D and 3D cultured models
  37. Physicochemical properties and some mineral concentration of milk samples from different animals and altitudes
  38. Copper(ii) complexes supported by modified azo-based ligands: Nucleic acid binding and molecular docking studies
  39. Diagnostic and therapeutic radioisotopes in nuclear medicine: Determination of gamma-ray transmission factors and safety competencies of high-dense and transparent glassy shields
  40. Calculation of NaI(Tl) detector efficiency using 226Ra, 232Th, and 40K radioisotopes: Three-phase Monte Carlo simulation study
  41. Isolation and identification of unstable components from Caesalpinia sappan by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography
  42. Quantification of biomarkers and evaluation of antioxidant, anti-inflammatory, and cytotoxicity properties of Dodonaea viscosa grown in Saudi Arabia using HPTLC technique
  43. Characterization of the elastic modulus of ceramic–metal composites with physical and mechanical properties by ultrasonic technique
  44. GC-MS analysis of Vespa velutina auraria Smith and its anti-inflammatory and antioxidant activities in vitro
  45. Texturing of nanocoatings for surface acoustic wave-based sensors for volatile organic compounds
  46. Insights into the molecular basis of some chalcone analogues as potential inhibitors of Leishmania donovani: An integrated in silico and in vitro study
  47. (1R,2S,5R)-5-Methyl-2-(propan-2-yl)cyclohexyl 4-amino-3-phenylbutanoate hydrochloride: Synthesis and anticonvulsant activity
  48. On the relative extraction rates of colour compounds and caffeine during brewing, an investigation of tea over time and temperature
  49. Characterization of egg shell powder-doped ceramic–metal composites
  50. Rapeseed oil-based hippurate amide nanocomposite coating material for anticorrosive and antibacterial applications
  51. Chemically modified Teucrium polium (Lamiaceae) plant act as an effective adsorbent tool for potassium permanganate (KMnO4) in wastewater remediation
  52. Efficiency analysis of photovoltaic systems installed in different geographical locations
  53. Risk prioritization model driven by success factor in the light of multicriteria decision making
  54. Theoretical investigations on the excited-state intramolecular proton transfer in the solvated 2-hydroxy-1-naphthaldehyde carbohydrazone
  55. Mechanical and gamma-ray shielding examinations of Bi2O3–PbO–CdO–B2O3 glass system
  56. Machine learning-based forecasting of potability of drinking water through adaptive boosting model
  57. The potential effect of the Rumex vesicarius water seeds extract treatment on mice before and during pregnancy on the serum enzymes and the histology of kidney and liver
  58. Impact of benzimidazole functional groups on the n-doping properties of benzimidazole derivatives
  59. Extraction of red pigment from Chinese jujube peel and the antioxidant activity of the pigment extracts
  60. Flexural strength and thermal properties of carbon black nanoparticle reinforced epoxy composites obtained from waste tires
  61. A focusing study on radioprotective and antioxidant effects of Annona muricata leaf extract in the circulation and liver tissue: Clinical and experimental studies
  62. Clinical comprehensive and experimental assessment of the radioprotective effect of Annona muricata leaf extract to prevent cellular damage in the ileum tissue
  63. Effect of WC content on ultrasonic properties, thermal and electrical conductivity of WC–Co–Ni–Cr composites
  64. Influence of various class cleaning agents for prosthesis on Co–Cr alloy surface
  65. The synthesis of nanocellulose-based nanocomposites for the effective removal of hexavalent chromium ions from aqueous solution
  66. Study on the influence of physical interlayers on the remaining oil production under different development modes
  67. Optimized linear regression control of DC motor under various disturbances
  68. Influence of different sample preparation strategies on hypothesis-driven shotgun proteomic analysis of human saliva
  69. Determination of flow distance of the fluid metal due to fluidity in ductile iron casting by artificial neural networks approach
  70. Investigation of mechanical activation effect on high-volume natural pozzolanic cements
  71. In vitro: Anti-coccidia activity of Calotropis procera leaf extract on Eimeria papillata oocysts sporulation and sporozoite
  72. Determination of oil composition of cowpea (Vigna unguiculata L.) seeds under influence of organic fertilizer forms
  73. Activated partial thromboplastin time maybe associated with the prognosis of papillary thyroid carcinoma
  74. Treatment of rat brain ischemia model by NSCs-polymer scaffold transplantation
  75. Lead and cadmium removal with native yeast from coastal wetlands
  76. Characterization of electroless Ni-coated Fe–Co composite using powder metallurgy
  77. Ferrate synthesis using NaOCl and its application for dye removal
  78. Antioxidant, antidiabetic, and anticholinesterase potential of Chenopodium murale L. extracts using in vitro and in vivo approaches
  79. Study on essential oil, antioxidant activity, anti-human prostate cancer effects, and induction of apoptosis by Equisetum arvense
  80. Experimental study on turning machine with permanent magnetic cutting tool
  81. Numerical simulation and mathematical modeling of the casting process for pearlitic spheroidal graphite cast iron
  82. Design, synthesis, and cytotoxicity evaluation of novel thiophene, pyrimidine, pyridazine, and pyridine: Griseofulvin heterocyclic extension derivatives
  83. Isolation and identification of promising antibiotic-producing bacteria
  84. Ultrasonic-induced reversible blood–brain barrier opening: Safety evaluation into the cellular level
  85. Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan
  86. Effect of calcium lactate in standard diet on selected markers of oxidative stress and inflammation in ovariectomized rats
  87. Identification of crucial salivary proteins/genes and pathways involved in pathogenesis of temporomandibular disorders
  88. Zirconium-modified attapulgite was used for removing of Cr(vi) in aqueous solution
  89. The stress distribution of different types of restorative materials in primary molar
  90. Reducing surface heat loss in steam boilers
  91. Deformation behavior and formability of friction stir processed DP600 steel
  92. Synthesis and characterization of bismuth oxide/commercial activated carbon composite for battery anode
  93. Phytochemical analysis of Ziziphus jujube leaf at different foliar ages based on widely targeted metabolomics
  94. Effects of in ovo injection of black cumin (Nigella sativa) extract on hatching performance of broiler eggs
  95. Separation and evaluation of potential antioxidant, analgesic, and anti-inflammatory activities of limonene-rich essential oils from Citrus sinensis (L.)
  96. Bioactivity of a polyhydroxy gorgostane steroid from Xenia umbellata
  97. BiCAM-based automated scoring system for digital logic circuit diagrams
  98. Analysis of standard systems with solar monitoring systems
  99. Structural and spectroscopic properties of voriconazole and fluconazole – Experimental and theoretical studies
  100. New plant resistance inducers based on polyamines
  101. Experimental investigation of single-lap bolted and bolted/bonded (hybrid) joints of polymeric plates
  102. Investigation of inlet air pressure and evaporative cooling of four different cogeneration cycles
  103. Review Articles
  104. Comprehensive review on synthesis, physicochemical properties, and application of activated carbon from the Arecaceae plants for enhanced wastewater treatment
  105. Research progress on speciation analysis of arsenic in traditional Chinese medicine
  106. Recent modified air-assisted liquid–liquid microextraction applications for medicines and organic compounds in various samples: A review
  107. An insight on Vietnamese bio-waste materials as activated carbon precursors for multiple applications in environmental protection
  108. Antimicrobial activities of the extracts and secondary metabolites from Clausena genus – A review
  109. Bioremediation of organic/heavy metal contaminants by mixed cultures of microorganisms: A review
  110. Sonodynamic therapy for breast cancer: A literature review
  111. Recent progress of amino acid transporters as a novel antitumor target
  112. Aconitum coreanum Rapaics: Botany, traditional uses, phytochemistry, pharmacology, and toxicology
  113. Corrigendum
  114. Corrigendum to “Petrology and geochemistry of multiphase post-granitic dikes: A case study from the Gabal Serbal area, Southwestern Sinai, Egypt”
  115. Corrigendum to “Design of a Robust sliding mode controller for bioreactor cultures in overflow metabolism via an interdisciplinary approach”
  116. Corrigendum to “Statistical analysis on the radiological assessment and geochemical studies of granite rocks in the north of Um Taghir area, Eastern Desert, Egypt”
  117. Corrigendum to “Aroma components of tobacco powder from different producing areas based on gas chromatography ion mobility spectrometry”
  118. Corrigendum to “Mechanical properties, elastic moduli, transmission factors, and gamma-ray-shielding performances of Bi2O3–P2O5–B2O3–V2O5 quaternary glass system”
  119. Erratum
  120. Erratum to “Copper(ii) complexes supported by modified azo-based ligands: Nucleic acid binding and molecular docking studies”
  121. Special Issue on Applied Biochemistry and Biotechnology (ABB 2021)
  122. Study of solidification and stabilization of heavy metals by passivators in heavy metal-contaminated soil
  123. Human health risk assessment and distribution of VOCs in a chemical site, Weinan, China
  124. Preparation and characterization of Sparassis latifolia β-glucan microcapsules
  125. Special Issue on the Conference of Energy, Fuels, Environment 2020
  126. Improving the thermal performance of existing buildings in light of the requirements of the EU directive 2010/31/EU in Poland
  127. Special Issue on Ethnobotanical, Phytochemical and Biological Investigation of Medicinal Plants
  128. Study of plant resources with ethnomedicinal relevance from district Bagh, Azad Jammu and Kashmir, Pakistan
  129. Studies on the chemical composition of plants used in traditional medicine in Congo
  130. Special Issue on Applied Chemistry in Agriculture and Food Science
  131. Strip spraying technology for precise herbicide application in carrot fields
  132. Special Issue on Pharmacology and Metabolomics of Ethnobotanical and Herbal Medicine
  133. Phytochemical profiling, antibacterial and antioxidant properties of Crocus sativus flower: A comparison between tepals and stigmas
  134. Antioxidant and antimicrobial properties of polyphenolics from Withania adpressa (Coss.) Batt. against selected drug-resistant bacterial strains
  135. Integrating network pharmacology and molecular docking to explore the potential mechanism of Xinguan No. 3 in the treatment of COVID-19
  136. Chemical composition and in vitro and in vivo biological assortment of fixed oil extracted from Ficus benghalensis L.
  137. A review of the pharmacological activities and protective effects of Inonotus obliquus triterpenoids in kidney diseases
  138. Ethnopharmacological study of medicinal plants in Kastamonu province (Türkiye)
  139. Protective effects of asperuloside against cyclophosphamide-induced urotoxicity and hematotoxicity in rats
  140. Special Issue on Essential Oil, Extraction, Phytochemistry, Advances, and Application
  141. Identification of volatile compounds and antioxidant, antibacterial, and antifungal properties against drug-resistant microbes of essential oils from the leaves of Mentha rotundifolia var. apodysa Briq. (Lamiaceae)
  142. Phenolic contents, anticancer, antioxidant, and antimicrobial capacities of MeOH extract from the aerial parts of Trema orientalis plant
  143. Chemical composition and antimicrobial activity of essential oils from Mentha pulegium and Rosmarinus officinalis against multidrug-resistant microbes and their acute toxicity study
  144. Special Issue on Marine Environmental Sciences and Significance of the Multidisciplinary Approaches
  145. An insightful overview of the distribution pattern of polycyclic aromatic hydrocarbon in the marine sediments of the Red Sea
  146. Antifungal–antiproliferative norcycloartane-type triterpenes from the Red Sea green alga Tydemania expeditionis
  147. Solvent effect, dipole moment, and DFT studies of multi donor–acceptor type pyridine derivative
  148. An extensive assessment on the distribution pattern of organic contaminants in the aerosols samples in the Middle East
  149. Special Issue on 4th IC3PE
  150. Energetics of carboxylic acid–pyridine heterosynthon revisited: A computational study of intermolecular hydrogen bond domination on phenylacetic acid–nicotinamide cocrystals
  151. A review: Silver–zinc oxide nanoparticles – organoclay-reinforced chitosan bionanocomposites for food packaging
  152. Green synthesis of magnetic activated carbon from peanut shells functionalized with TiO2 photocatalyst for Batik liquid waste treatment
  153. Coagulation activity of liquid extraction of Leucaena leucocephala and Sesbania grandiflora on the removal of turbidity
  154. Hydrocracking optimization of palm oil over NiMoO4/activated carbon catalyst to produce biogasoline and kerosine
  155. Special Issue on Pharmacology and metabolomics of ethnobotanical and herbal medicine
  156. Cynarin inhibits PDGF-BB-induced proliferation and activation in hepatic stellate cells through PPARγ
  157. Special Issue on The 1st Malaysia International Conference on Nanotechnology & Catalysis (MICNC2021)
  158. Surfactant evaluation for enhanced oil recovery: Phase behavior and interfacial tension
  159. Topical Issue on phytochemicals, biological and toxicological analysis of aromatic medicinal plants
  160. Phytochemical analysis of leaves and stems of Physalis alkekengi L. (Solanaceae)
  161. Phytochemical and pharmacological profiling of Trewia nudiflora Linn. leaf extract deciphers therapeutic potentials against thrombosis, arthritis, helminths, and insects
  162. Pergularia tomentosa coupled with selenium nanoparticles salvaged lead acetate-induced redox imbalance, inflammation, apoptosis, and disruption of neurotransmission in rats’ brain
  163. Protective effect of Allium atroviolaceum-synthesized SeNPs on aluminum-induced brain damage in mice
  164. Mechanism study of Cordyceps sinensis alleviates renal ischemia–reperfusion injury
  165. Plant-derived bisbenzylisoquinoline alkaloid tetrandrine prevents human podocyte injury by regulating the miR-150-5p/NPHS1 axis
  166. Network pharmacology combined with molecular docking to explore the anti-osteoporosis mechanisms of β-ecdysone derived from medicinal plants
  167. Chinese medicinal plant Polygonum cuspidatum ameliorates silicosis via suppressing the Wnt/β-catenin pathway
  168. Special Issue on Advanced Nanomaterials for Energy, Environmental and Biological Applications - Part I
  169. Investigation of improved optical and conductivity properties of poly(methyl methacrylate)–MXenes (PMMA–MXenes) nanocomposite thin films for optoelectronic applications
  170. Special Issue on Applied Biochemistry and Biotechnology (ABB 2022)
  171. Model predictive control for precision irrigation of a Quinoa crop
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2025 De Gruyter Brill
Heruntergeladen am 19.10.2025 von https://www.degruyterbrill.com/document/doi/10.1515/chem-2022-0236/html
Button zum nach oben scrollen