LINC00152 knock-down suppresses esophageal cancer by EGFR signaling pathway
-
Yan Ding
Abstract
Aim
This study aims to explain the role and mechanism of lncRNA LINC00152 in esophageal cancer.
Methods
The 30 pairs of esophageal cancer and adjacent normal tissues were collected and measuring the lncRNA LINC00152 expression by ISH and RT-qPCR assay. In the next cell experiment, Eca 109 and Kyse 150 cells were divided into 3 groups: NC group were treated with non-treatment; BL group were transfected with empty vector and lncRNA group were transfected with lncRNA LINC00152. The cells proliferation were measured by MTT assay; the cells apoptosis and cell cycle were evaluated by flow cytometry. The relative proteins expressions were measured by WB assay.
Results
Compared with NC groups, the cell proliferation rate of lncRNA groups were significantly suppressed (P<0.05, respectively); the cell apoptosis and G1 phase rates were significantly enhanced in the lncRNA groups (P<0.05, respectively). In the proteins expressions, the EGFR, PI3K and AKT proteins expressions of lncRNA group were significantly inhibited and the P21 proteins expressions were significantly stimulated in the lncRNA groups compared with those of NC groups in Eca 109 and Kyse 150 cells.
Conclusion
The lncRNA LINC00152 had anti-tumor effects on esophageal cancer in the Eca 109 and Kyse 150 cells, the mechanisms were relative with EGFR pathway.
1 Introduction
Esophageal cancer is a common gastrointestinal malignancy [1], which accounts for more than 70% of esophageal cancer patients worldwide [2]. In recent years, many studies had confirmed that lncRNas were closely related to human tumors, and lncRNA had unique biological functions in cancer suppression and cancer promoting pathways. Therefore, lncRNAs had become a new hotspot in cancer research in recent years. Related studies reported that lncRNA is closely related to the occurrence, development, metastasis and recurrence of esophageal cancer [3, 4, 5]. Therefore, the study of the expression and its mechanism of lncRNA has become an important theoretical basis for molecular diagnosis and molecular targeted therapy of esophageal cancer. LncRNA LINC00152 plays an important role in lncRNAs family. Some relevant studies reported that the lncRNA LINC00152 was over-expression in some cancer tissues by regulation of relative pathways to promote cancer cells proliferation [6, 7, 8, 9]. In this study, we discussed the effects and mechanisms of lncRNA LINC00152 knockdown in Eca 109 and Kyse 150 cells which were two kinds of esophageal cancer in vitro study.
2 Materials and methods
2.1 Clinical sample
Thirty pairs of Non-tumor and esophageal cancer tissues were taken from esophageal cancer patients who received treatment in Nanjing University of Chinese Medicine from October 2015 to December 2016. All patients were primary esophageal carcinoma and un-treated before the operation. The tissues were resection and quickly stored in the 10% polyoxymethylene to fix until using.
2.2 In situ hybridization (ISH) assay
The tissues were embedded by paraffin and section as 5μm, sections dewaxing by xylene, after gradient alcohol hydration, using fresh 3% H2O2 to close and place at room temperature for 5-10 min; washing by distilled water (2 min×3 times); adding a drop fresh pepsin which was dilution by 30 g/l citric acid to incubate in the thermostat box for 10-30 min; ISH PBS was used to wash (5 min×3 times), washing by distilled water for 2 min; adding pre-hybrids as 20 μl/slice to place in the wet box to culture in the thermostat box (40°C) for 3h; adding pre-hybrids as 20 μl/slice, special cover film for in-situ hybridization and place in the wet box in thermostat box (40°C) overnight; Gently uncover the special cover film of the in situ hybridization (2×SSC buffer solution as 5 min×2 times; 0.5×SSC buffer solution for 15 min; 0.2×SSC buffer solution for 15 min); adding closed liquid as 30μl/slice to culture for 30 min at 37°C; getting rid of the excess liquid, adding 30μl/slice biotinylated rat digoxin to culture for 1 h at 37°C; ISH PBS was used to wash (5 min×4 times); adding SABC 30μl/slice to culture for 30 min at 37°C; washing by ISH PBS (5 min×4 times); adding fresh DAB to color, washing by water; Gradient alcohol dehydration, Xylene transparent, Neutral gum seal. Using image analyze software to analysis the IOD of different tissues.
2.3 RT-qPCR assay
Total RNA was extracted from clinical samples using Trizol reagent (Thermo Fisher Scientific) following the protocols of manufacturer. For the measurement of linc00152 mRNA level, RNA was reversely transcribed into cDNA using M-MLV Reverse Transcriptase (Thermo Fisher Scientific) together with random primers and then cDNA was used for qPCR detection by PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific) along with specific quantitative primers. The quantitative PCR primers were presented as follows: LINC00152 F: 5ʹ-GAAGGTGTCGGCAAGATC-3ʹ; R: 5ʹ-TCGGTGTCTGTCATATTCG-3ʹ. β-actin: F: 5ʹ-AAAGACCT-GTACGCCAACAC-3ʹ; R: 5ʹ-GTCATACTCCTGCTTGCTGAT-3ʹ.
2.4 Cell culture and grouping
The Eca 109 and Kyse 150 cells, two kinds of esophageal cancer cell lines purchased from ATCC (USA), were cultured by RPMI1640 medium (Sigma, USA) contained with 10% fetal bovine serum (FBS) (Sigma, USA) in incubator (37°C, 5% CO2). When the cells reached 90% fluent, 0.25% trypsin was added to digest the cells and centrifuged to discard the supernatant. The suspension were added to cell culture medium and the incubated into the cell flask and future culture. Respectively dividing the Eca 109 and Kyse 150 cells into 3 groups: Normal control (NC) groups which were treated with non-treatment; Blank (BL) groups which were transfected with empty vector (GenScript (Nanjing) Co., Ltd., China) and si-lncRNA groups which were transfected with si-lncRNA LINC00152 (GenScript (Nanjing) Co., Ltd., China).
2.5 MTT assay
The Eca 109 or Kyse 150 cells of different groups were collected, the cell concentration of different groups were adjusted as 1×105 cells/ml, the cells were inoculated in the 96-well plate, 200 μl cell suspension were added into every holes, placed into incubator at 37°C for 48 h, 20 μl of MTT solution (5mg/ml) was added into every well, cultured in the incubator at 37℃ for 4 h, after discarding the supernatant, adding the 150 μl DMSO solution, Oscillatory reaction for 10 min, The absorbance (A) value were measured at 490 nm. The cell proliferation rates were measured.
2.6 The cell apoptosis rate of different groups by flow cytometry
The Eca 109 or Kyse 150 cells of different groups which were treated by different methods for 48 h were collected and adjusted as 1×105 cell/ml. Taking 1 ml cell suspension, added Annexin-V buffer solution, fully mixed, added the 5 μl Propidium iodide (PI) and Annexin-V-FITC, incubated avoid light for 20min, the cell apoptosis rate of different groups were measured by flow cytometry, the experiments were repeated for 3 times.
2.7 Cell cycle measurement by flow cytometry
The Eca 109 or Kyse 150 cells of different groups which were treated by different methods for 48 h were collected and adjustment as 1×105 cell/ml. The cells were washed by pre-cool PBS twice, the pre-cool 70% ethanol was added to fix at 4℃ overnight, washed by PBS once. After this, the centrifugation collection fixed overnight, 500 μl PBS was added to suspension cell, adding 20 μl RNase A solution and 0.2% Triton X-100. It was then cultured in a 37℃ water-bath for 30 min; added 400 μl PI Staining solution, and mixed, Incubation under light conditions at 4℃ for 30 min. The cell cycle were measured at 488 nm by Flow Cytometry. The experiments were repeated for 3 times.
2.8 Western blot (WB) assay
The Eca 109 or Kyse 150 cells of different groups which were logarithmic growth phase was collection. 3 ml Trizol cell lysate was added into the cells, incubated on ice for 10 min, transfer to the centrifuge tube, after centrifugation, the supernatant was transferred into the EP tube. Detecting protein concentration according to the BCA protein concentration kit. Mixed the protein with sample buffer and boiled for 5 min at 100°C, added into SDS-PAGE electrophoresis gel, 50 μl/well. After electrophoresis, proteins were transferred to PVDF (Millipore, Bedford, MA, USA) membrane at 4°C. After blocking, the membrane was incubated with primary antibodies: EGFR (1:800) (Abcam, USA), PI3K (1:800) (Abcam, USA), AKT (1:800) (Abcam, USA), P21 (1:800) (Abcam, USA) and GAPDH (1:1000) (Abcam, USA). Followed by incubation with secondary antibody (1:1000) (Abcam, USA). EGFR, PI3K, AKT and P21 gray value of protein bands were measured by Ge-1 Pro Analyzer (ver 3.0) software.
2.9 Statistical analysis
The data of this study were analyzed by SPSS 19.0 software, the data were expressed as Mean±SD (standard deviation). T-test was used to compare the differences between groups. The one-way ANOVA was used to analyze the differences between groups. The LSD-t test was used for multiple comparisons between groups, the value was bilateral probability, and the test level was α=0.05. P<0.05 indicated that the difference was statistically significant.
Ethical approval: The research related to human use has been complied with all the relevant national regulations, institutional policies and in accordance the tenets of the Helsinki Declaration, and has been approved by the authors' institutional review board or equivalent committee.
3 Results
3.1 LINC00152 expression in different tissues
Compared with no-tumor tissues, the LINC00152 expression was significantly increased in esophageal cancer tissues (P＜0.001). By RT-qPCR, the result was shown that LINC00152 mRNA expression of esophageal cancer tissues were significantly up-regulated compared with that of non-tumor tissues. Depending on these results, we inferred that LINC00152 might be an oncology factor in esophageal cancer occur and development. The relative data was shown in Figure 1.
The LINC00152 expression of different tissues by ISH and RT-qPCR (×100)
***:P＜0.001, compared with no-tumor tissues
3.2 LINC 00152 affects cell proliferation rate
To investigate the effect of the lncRNA LINC00152 on the esophageal cancer proliferation, the cells proliferation rates we measured by MTT assay. The relative results found that the lncRNA LINC00152 knockdown (si-lncRNA groups) suppressed cells proliferation compared with that of NC groups in Eca 109 (P<0.05,69.69±5.65 vs.127.33±5.02, Figure 2A) and Kyse 150 (P<0.05,71.50±4.48 vs.130.58±6.00, Figure 2B). The relative data were shown in Figure 2.
The cell proliferation rate of difference groups in Eca 109 and Kyse 150 cells. A. The Eca 109 cell proliferation rate of difference groups by MTT assay.
NC: Normal control group; BL: Blank group; si-lncRNA: si-lncRNA LINC00152 group
***: P<0.05, compared with NC group. B. The Kyse 150 cell proliferation rate of difference groups by MTT assay. ***: P<0.05, compared with NC group.
3.3 The cell apoptosis rates of different groups
To evaluate the effects of lncRNA LINC00152 in esophageal cancer apoptosis, we measured the cell apoptosis rates of difference groups by flow cytometry. Compared with NC groups, the cell apoptosis rates of si-lncRNA groups, which were knocked-down the lncRNA LINC00152, were significantly enhanced in Eca 109 (P<0.05, Figure 3A) and Kyse 150 (P<0.05, Figure 3B). The relative data were shown in Figure 3.
Cell apoptosis measurement by flow cytometry in Eca 109 and Kyse 150 cells.
NC: Normal control group; BL: Blank group; si-lncRNA: si-lncRNA LINC00152 group
A. The Eca 109 cell apoptosis rate of difference groups by flow cytometry. ***: P<0.05, compared with NC group. B. The Kyse 150 cell apoptosis rate of difference groups by flow cytometry. ***: P<0.05, compared with NC group.
3.4 The cell cycle by flow cytometry
To investigate the effects of lncRNA LINC00152 in esophageal cancer cell cycle, the cell cycle were evaluated by flow cytometry in different groups. Compared with NC groups, the G1 phase rate of si-lncRNA groups which the lncRNA LINC00152 were down-regulated were significantly increased in Eca 109 (P<0.05, Figure 4A) and Kyse 150 (P<0.05, Figure 4B). The relative data were shown in Figure 4.
The cell cycle rates of difference groups in Eca 109 and Kyse 150 cells.
NC: Normal control group; BL: Blank group; si-lncRNA: si-lncRNA LINC00152 group
A. The Eca 109 cell cycle rates of difference groups by flow cytometry. ***: P<0.05, compared with NC group. B. The Kyse 150 cell cycle rates of difference groups by flow cytometry. ***: P<0.05, compared with NC group.
3.5 LINC00152 affects relative protein expression
The EGFR, PI3K and AKT proteins expressions of si-ln-cRNA groups were significantly suppressed compare with those proteins expression of NC groups in Eca 109 (P<0.05, Figure 5A) and Kyse 150 (P<0.05, Figure 5B). Meanwhile, the P21 protein expression of si-lncRNA group was significantly increased compared with that of NC groups in Eca 109 (P<0.05, Figure 5A) and Kyse 150 (P<0.05, Figure 5B).
The relative proteins expressions of difference groups in Eca 109 and Kyse 150 cells.
NC: Normal control group; BL: Blank group; si-lncRNA: si-lncRNA LINC00152 group
A. The relative proteins expressions of difference groups in Eca 109 by WB assay. ***: P<0.05, compared with NC group. B. The relative proteins expressions of difference groups in Kyse 150 by WB assay. ***: P<0.05, compared with NC group.
4 Discussion
As an important role of cancer occurrence and development, lncRNAs were considered as potentially prime targets to cancer therapy. The lncRNAs are not only biomarkers, but also to be included in the prognosis of patients with various cancers [6, 7, 8, 9, 10]. Many previous studies reported that lncRNA LINC00152 were up-regulated in the cancer tissues including hepatocellular carcinoma, gastric cancer and clear cell renal carcinoma and closely related with cancer progression [11, 12, 13, 14, 15, 16]. In our present study, the results shown that knock-down of lncRNA LINC00152 suppressed esophageal cancer cell proliferation and enhanced esophageal cancer cell apoptosis. Furthermore, we analyzed the related proteins expressions with lncRNA LINC00152 down-regulation. The results showed that the expression of EGFR, PI3K and AKT proteins are inhibited, and the expression of p21 protein was stimulated by the down-expression of lncrna linc0152 in vitro study.
EGFR is a transmembrane protein on the cell surface, which is activated by homologous or heterogeneous forms. It promotes cell proliferation, differentiation and development by activating intracellular signaling pathways such as PI3K-AKT, MEK/ERK and STAT3 [17, 18, 19, 20]. PI3K/AKT pathway is a key signaling pathway in the body, and participates in the process of tumor development through regulating cell proliferation and apoptosis [21, 22, 23]. PI3K/AKT pathway also suppressed P21 expression to improve cancer cell proliferation and inhibit cell apoptosis [24]. P21 protein is the most widely known cyclin kinase inhibitor [25]. Relative studies found that P21 over-expression could keep the cell cycle in the G1 phase that might lead cell apoptosis increasing [26, 27]. In our present study, the results were shown that lncRNA LINC00152 knock-down could inhibit EGFR/PI3K/AKT pathway and stimulate P21 expression in esophageal cancer cell lines (Eca 109 and Kyse 150) in vitro study. That might be the mechanism of lncRNA LINC00152.
In conclusion, lncRNA LINC00152 knock-down might suppress esophageal cancer cell lines Eca 109 and Kyse 150 cell proliferation and induce cell apoptosis. The mechanism of lncRNA LINC00152 might be collected with EGFR/PI3K/AKT and P21 in vitro study. Depending on these results, we inferred that lncRNA LINC00152 down-regulation have anti-tumor effects via inhibiting EGFR/PI3K/AKT pathway and enhancing P21 expressions in esophageal cancer.
Conflict of interest
Conflict of interest statement: Authors state no conflict of interest.
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© 2020 Yan Ding et al., published by De Gruyter
This work is licensed under the Creative Commons Attribution 4.0 International License.
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- 72 hour Holter monitoring, 7 day Holter monitoring, and 30 day intermittent patient-activated heart rhythm recording in detecting arrhythmias in cryptogenic stroke patients free from arrhythmia in a screening 24 h Holter
- FOXK2 downregulation suppresses EMT in hepatocellular carcinoma
- Case Report
- Total parenteral nutrition-induced Wernicke’s encephalopathy after oncologic gastrointestinal surgery
- Research Article
- Clinical prediction for outcomes of patients with acute-on-chronic liver failure associated with HBV infection: A new model establishment
- Case Report
- Combination of chest CT and clinical features for diagnosis of 2019 novel coronavirus pneumonia
- Research Article
- Clinical significance and potential mechanisms of miR-223-3p and miR-204-5p in squamous cell carcinoma of head and neck: a study based on TCGA and GEO
- Review Article
- Hemoperitoneum caused by spontaneous rupture of hepatocellular carcinoma in noncirrhotic liver. A case report and systematic review
- Research Article
- Voltage-dependent anion channels mediated apoptosis in refractory epilepsy
- Prognostic factors in stage I gastric cancer: A retrospective analysis
- Circulating irisin is linked to bone mineral density in geriatric Chinese men
- Case Report
- A family study of congenital dysfibrinogenemia caused by a novel mutation in the FGA gene: A case report
- Research Article
- CBCT for estimation of the cemento-enamel junction and crestal bone of anterior teeth
- Case Report
- Successful de-escalation antibiotic therapy using cephamycins for sepsis caused by extended-spectrum beta-lactamase-producing Enterobacteriaceae bacteremia: A sequential 25-case series
- Research Article
- Influence factors of extra-articular manifestations in rheumatoid arthritis
- Assessment of knowledge of use of electronic cigarette and its harmful effects among young adults
- Predictive factors of progression to severe COVID-19
- Procedural sedation and analgesia for percutaneous trans-hepatic biliary drainage: Randomized clinical trial for comparison of two different concepts
- Acute chemoradiotherapy toxicity in cervical cancer patients
- IGF-1 regulates the growth of fibroblasts and extracellular matrix deposition in pelvic organ prolapse
- NANOG regulates the proliferation of PCSCs via the TGF-β1/SMAD pathway
- An immune-relevant signature of nine genes as a prognostic biomarker in patients with gastric carcinoma
- Computer-aided diagnosis of skin cancer based on soft computing techniques
- MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B
- miR-300/FA2H affects gastric cancer cell proliferation and apoptosis
- Hybrid treatment of fibroadipose vascular anomaly: A case report
- Surgical treatment for common hepatic aneurysm. Original one-step technique
- Neuropsychiatric symptoms, quality of life and caregivers’ burden in dementia
- Predictor of postoperative dyspnea for Pierre Robin Sequence infants
- Long non-coding RNA FOXD2-AS1 promotes cell proliferation, metastasis and EMT in glioma by sponging miR-506-5p
- Analysis of expression and prognosis of KLK7 in ovarian cancer
- Circular RNA circ_SETD2 represses breast cancer progression via modulating the miR-155-5p/SCUBE2 axis
- Glial cell induced neural differentiation of bone marrow stromal cells
- Case Report
- Moraxella lacunata infection accompanied by acute glomerulonephritis
- Research Article
- Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS
- Case Report
- Endometrial cancer in a renal transplant recipient: A case report
- Research Article
- Downregulation of lncRNA FGF12-AS2 suppresses the tumorigenesis of NSCLC via sponging miR-188-3p
- Case Report
- Splenic abscess caused by Streptococcus anginosus bacteremia secondary to urinary tract infection: a case report and literature review
- Research Article
- Advances in the role of miRNAs in the occurrence and development of osteosarcoma
- Rheumatoid arthritis increases the risk of pleural empyema
- Effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA
- LncRNA NEAT1 promotes gastric cancer progression via miR-1294/AKT1 axis
- Key pathways in prostate cancer with SPOP mutation identified by bioinformatic analysis
- Comparison of low-molecular-weight heparins in thromboprophylaxis of major orthopaedic surgery – randomized, prospective pilot study
- Case Report
- A case of SLE with COVID-19 and multiple infections
- Research Article
- Circular RNA hsa_circ_0007121 regulates proliferation, migration, invasion, and epithelial–mesenchymal transition of trophoblast cells by miR-182-5p/PGF axis in preeclampsia
- SRPX2 boosts pancreatic cancer chemoresistance by activating PI3K/AKT axis
- Case Report
- A case report of cervical pregnancy after in vitro fertilization complicated by tuberculosis and a literature review
- Review Article
- Serrated lesions of the colon and rectum: Emergent epidemiological data and molecular pathways
- Research Article
- Biological properties and therapeutic effects of plant-derived nanovesicles
- Case Report
- Clinical characterization of chromosome 5q21.1–21.3 microduplication: A case report
- Research Article
- Serum calcium levels correlates with coronary artery disease outcomes
- Rapunzel syndrome with cholangitis and pancreatitis – A rare case report
- Review Article
- A review of current progress in triple-negative breast cancer therapy
- Case Report
- Peritoneal-cutaneous fistula successfully treated at home: A case report and literature review
- Research Article
- Trim24 prompts tumor progression via inducing EMT in renal cell carcinoma
- Degradation of connexin 50 protein causes waterclefts in human lens
- GABRD promotes progression and predicts poor prognosis in colorectal cancer
- The lncRNA UBE2R2-AS1 suppresses cervical cancer cell growth in vitro
- LncRNA FOXD3-AS1/miR-135a-5p function in nasopharyngeal carcinoma cells
- MicroRNA-182-5p relieves murine allergic rhinitis via TLR4/NF-κB pathway
Articles in the same Issue
- Research Article
- MicroRNA-451b participates in coronary heart disease by targeting VEGFA
- Case Report
- A combination therapy for Kawasaki disease with severe complications: a case report
- Vitamin E for prevention of biofilm-caused Healthcare-associated infections
- Research Article
- Differential diagnosis: retroperitoneal fibrosis and oncological diseases
- Optimization of the Convolutional Neural Networks for Automatic Detection of Skin Cancer
- NEAT1 promotes LPS-induced inflammatory injury in macrophages by regulating miR-17-5p/TLR4
- Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients
- Effects of extracorporeal magnetic stimulation in fecal incontinence
- Case Report
- Mixed germ cell tumor of the endometrium: a case report and literature review
- Bowel perforation after ventriculoperitoneal-shunt placement: case report and review of the literature
- Research Article
- Prognostic value of lncRNA HOTAIR in colorectal cancer : a meta-analysis
- Case Report
- Treatment of insulinomas by laparoscopic radiofrequency ablation: case reports and literature review
- Research Article
- The characteristics and nomogram for primary lung papillary adenocarcinoma
- Undiagnosed pheochromocytoma presenting as a pancreatic tumor: A case report
- Bioinformatics Analysis of the Expression of ATP binding cassette subfamily C member 3 (ABCC3) in Human Glioma
- Diagnostic value of recombinant heparin-binding hemagglutinin adhesin protein in spinal tuberculosis
- Primary cutaneous DLBCL non-GCB type: challenges of a rare case
- LINC00152 knock-down suppresses esophageal cancer by EGFR signaling pathway
- Case Report
- Life-threatening anaemia in patient with hereditary haemorrhagic telangiectasia (Rendu-Osler-Weber syndrome)
- Research Article
- QTc interval predicts disturbed circadian blood pressure variation
- Shoulder ultrasound in the diagnosis of the suprascapular neuropathy in athletes
- The number of negative lymph nodes is positively associated with survival in esophageal squamous cell carcinoma patients in China
- Differentiation of pontine infarction by size
- RAF1 expression is correlated with HAF, a parameter of liver computed tomographic perfusion, and may predict the early therapeutic response to sorafenib in advanced hepatocellular carcinoma patients
- LncRNA ZEB1-AS1 regulates colorectal cancer cells by miR-205/YAP1 axis
- Tissue coagulation in laser hemorrhoidoplasty – an experimental study
- Classification of pathological types of lung cancer from CT images by deep residual neural networks with transfer learning strategy
- Enhanced Recovery after Surgery for Lung Cancer Patients
- Case Report
- Streptococcus pneumoniae-associated thrombotic microangiopathy in an immunosuppressed adult
- Research Article
- The characterization of Enterococcus genus: resistance mechanisms and inflammatory bowel disease
- Case Report
- Inflammatory fibroid polyp: an unusual cause of abdominal pain in the upper gastrointestinal tract A case report
- Research Article
- microRNA-204-5p participates in atherosclerosis via targeting MMP-9
- LncRNA LINC00152 promotes laryngeal cancer progression by sponging miR-613
- Can keratin scaffolds be used for creating three-dimensional cell cultures?
- miRNA-186 improves sepsis induced renal injury via PTEN/PI3K/AKT/P53 pathway
- Case Report
- Delayed bowel perforation after routine distal loopogram prior to ileostomy closure
- Research Article
- Diagnostic accuracy of MALDI-TOF mass spectrometry for the direct identification of clinical pathogens from urine
- The R219K polymorphism of the ATP binding cassette subfamily A member 1 gene and susceptibility to ischemic stroke in Chinese population
- miR-92 regulates the proliferation, migration, invasion and apoptosis of glioma cells by targeting neogenin
- Clinicopathological features of programmed cell death-ligand 1 expression in patients with oral squamous cell carcinoma
- NF2 inhibits proliferation and cancer stemness in breast cancer
- Body composition indices and cardiovascular risk in type 2 diabetes. CV biomarkers are not related to body composition
- S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53
- Review Article
- Focus on localized laryngeal amyloidosis: management of five cases
- Research Article
- NEAT1 aggravates sepsis-induced acute kidney injury by sponging miR-22-3p
- Pericentric inversion in chromosome 1 and male infertility
- Increased atherogenic index in the general hearing loss population
- Prognostic role of SIRT6 in gastrointestinal cancers: a meta-analysis
- The complexity of molecular processes in osteoarthritis of the knee joint
- Interleukin-6 gene −572 G > C polymorphism and myocardial infarction risk
- Case Report
- Severe anaphylactic reaction to cisatracurium during anesthesia with cross-reactivity to atracurium
- Research Article
- Rehabilitation training improves nerve injuries by affecting Notch1 and SYN
- Case Report
- Myocardial amyloidosis following multiple myeloma in a 38-year-old female patient: A case report
- Research Article
- Identification of the hub genes RUNX2 and FN1 in gastric cancer
- miR-101-3p sensitizes non-small cell lung cancer cells to irradiation
- Distinct functions and prognostic values of RORs in gastric cancer
- Clinical impact of post-mortem genetic testing in cardiac death and cardiomyopathy
- Efficacy of pembrolizumab for advanced/metastatic melanoma: a meta-analysis
- Review Article
- The role of osteoprotegerin in the development, progression and management of abdominal aortic aneurysms
- Research Article
- Identification of key microRNAs of plasma extracellular vesicles and their diagnostic and prognostic significance in melanoma
- miR-30a-3p participates in the development of asthma by targeting CCR3
- microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9
- Bladder-embedded ectopic intrauterine device with calculus
- Case Report
- Mycobacterial identification on homogenised biopsy facilitates the early diagnosis and treatment of laryngeal tuberculosis
- Research Article
- The will of young minors in the terminal stage of sickness: A case report
- Extended perfusion protocol for MS lesion quantification
- Identification of four genes associated with cutaneous metastatic melanoma
- Case Report
- Thalidomide-induced serious RR interval prolongation (longest interval >5.0 s) in multiple myeloma patient with rectal cancer: A case report
- Research Article
- Voluntary exercise and cardiac remodeling in a myocardial infarction model
- Electromyography as an intraoperative test to assess the quality of nerve anastomosis – experimental study on rats
- Case Report
- CT findings of severe novel coronavirus disease (COVID-19): A case report of Heilongjiang Province, China
- Commentary
- Directed differentiation into insulin-producing cells using microRNA manipulation
- Research Article
- Culture-negative infective endocarditis (CNIE): impact on postoperative mortality
- Extracorporeal shock wave therapy for the treatment of chronic pelvic pain syndrome
- Plasma microRNAs in human left ventricular reverse remodelling
- Bevacizumab for non-small cell lung cancer patients with brain metastasis: A meta-analysis
- Risk factors for cerebral vasospasm in patients with aneurysmal subarachnoid hemorrhage
- Problems and solutions of personal protective equipment doffing in COVID-19
- Evaluation of COVID-19 based on ACE2 expression in normal and cancer patients
- Review Article
- Gastroenterological complications in kidney transplant patients
- Research Article
- CXCL13 concentration in latent syphilis patients with treatment failure
- A novel age-biomarker-clinical history prognostic index for heart failure with reduced left ventricular ejection fraction
- Case Report
- Clinicopathological analysis of composite lymphoma: A two-case report and literature review
- Trastuzumab-induced thrombocytopenia after eight cycles of trastuzumab treatment
- Research Article
- Inhibition of vitamin D analog eldecalcitol on hepatoma in vitro and in vivo
- CCTs as new biomarkers for the prognosis of head and neck squamous cancer
- Effect of glucagon-like peptide-1 receptor agonists on adipokine level of nonalcoholic fatty liver disease in rats fed high-fat diet
- 72 hour Holter monitoring, 7 day Holter monitoring, and 30 day intermittent patient-activated heart rhythm recording in detecting arrhythmias in cryptogenic stroke patients free from arrhythmia in a screening 24 h Holter
- FOXK2 downregulation suppresses EMT in hepatocellular carcinoma
- Case Report
- Total parenteral nutrition-induced Wernicke’s encephalopathy after oncologic gastrointestinal surgery
- Research Article
- Clinical prediction for outcomes of patients with acute-on-chronic liver failure associated with HBV infection: A new model establishment
- Case Report
- Combination of chest CT and clinical features for diagnosis of 2019 novel coronavirus pneumonia
- Research Article
- Clinical significance and potential mechanisms of miR-223-3p and miR-204-5p in squamous cell carcinoma of head and neck: a study based on TCGA and GEO
- Review Article
- Hemoperitoneum caused by spontaneous rupture of hepatocellular carcinoma in noncirrhotic liver. A case report and systematic review
- Research Article
- Voltage-dependent anion channels mediated apoptosis in refractory epilepsy
- Prognostic factors in stage I gastric cancer: A retrospective analysis
- Circulating irisin is linked to bone mineral density in geriatric Chinese men
- Case Report
- A family study of congenital dysfibrinogenemia caused by a novel mutation in the FGA gene: A case report
- Research Article
- CBCT for estimation of the cemento-enamel junction and crestal bone of anterior teeth
- Case Report
- Successful de-escalation antibiotic therapy using cephamycins for sepsis caused by extended-spectrum beta-lactamase-producing Enterobacteriaceae bacteremia: A sequential 25-case series
- Research Article
- Influence factors of extra-articular manifestations in rheumatoid arthritis
- Assessment of knowledge of use of electronic cigarette and its harmful effects among young adults
- Predictive factors of progression to severe COVID-19
- Procedural sedation and analgesia for percutaneous trans-hepatic biliary drainage: Randomized clinical trial for comparison of two different concepts
- Acute chemoradiotherapy toxicity in cervical cancer patients
- IGF-1 regulates the growth of fibroblasts and extracellular matrix deposition in pelvic organ prolapse
- NANOG regulates the proliferation of PCSCs via the TGF-β1/SMAD pathway
- An immune-relevant signature of nine genes as a prognostic biomarker in patients with gastric carcinoma
- Computer-aided diagnosis of skin cancer based on soft computing techniques
- MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B
- miR-300/FA2H affects gastric cancer cell proliferation and apoptosis
- Hybrid treatment of fibroadipose vascular anomaly: A case report
- Surgical treatment for common hepatic aneurysm. Original one-step technique
- Neuropsychiatric symptoms, quality of life and caregivers’ burden in dementia
- Predictor of postoperative dyspnea for Pierre Robin Sequence infants
- Long non-coding RNA FOXD2-AS1 promotes cell proliferation, metastasis and EMT in glioma by sponging miR-506-5p
- Analysis of expression and prognosis of KLK7 in ovarian cancer
- Circular RNA circ_SETD2 represses breast cancer progression via modulating the miR-155-5p/SCUBE2 axis
- Glial cell induced neural differentiation of bone marrow stromal cells
- Case Report
- Moraxella lacunata infection accompanied by acute glomerulonephritis
- Research Article
- Diagnosis of complication in lung transplantation by TBLB + ROSE + mNGS
- Case Report
- Endometrial cancer in a renal transplant recipient: A case report
- Research Article
- Downregulation of lncRNA FGF12-AS2 suppresses the tumorigenesis of NSCLC via sponging miR-188-3p
- Case Report
- Splenic abscess caused by Streptococcus anginosus bacteremia secondary to urinary tract infection: a case report and literature review
- Research Article
- Advances in the role of miRNAs in the occurrence and development of osteosarcoma
- Rheumatoid arthritis increases the risk of pleural empyema
- Effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA
- LncRNA NEAT1 promotes gastric cancer progression via miR-1294/AKT1 axis
- Key pathways in prostate cancer with SPOP mutation identified by bioinformatic analysis
- Comparison of low-molecular-weight heparins in thromboprophylaxis of major orthopaedic surgery – randomized, prospective pilot study
- Case Report
- A case of SLE with COVID-19 and multiple infections
- Research Article
- Circular RNA hsa_circ_0007121 regulates proliferation, migration, invasion, and epithelial–mesenchymal transition of trophoblast cells by miR-182-5p/PGF axis in preeclampsia
- SRPX2 boosts pancreatic cancer chemoresistance by activating PI3K/AKT axis
- Case Report
- A case report of cervical pregnancy after in vitro fertilization complicated by tuberculosis and a literature review
- Review Article
- Serrated lesions of the colon and rectum: Emergent epidemiological data and molecular pathways
- Research Article
- Biological properties and therapeutic effects of plant-derived nanovesicles
- Case Report
- Clinical characterization of chromosome 5q21.1–21.3 microduplication: A case report
- Research Article
- Serum calcium levels correlates with coronary artery disease outcomes
- Rapunzel syndrome with cholangitis and pancreatitis – A rare case report
- Review Article
- A review of current progress in triple-negative breast cancer therapy
- Case Report
- Peritoneal-cutaneous fistula successfully treated at home: A case report and literature review
- Research Article
- Trim24 prompts tumor progression via inducing EMT in renal cell carcinoma
- Degradation of connexin 50 protein causes waterclefts in human lens
- GABRD promotes progression and predicts poor prognosis in colorectal cancer
- The lncRNA UBE2R2-AS1 suppresses cervical cancer cell growth in vitro
- LncRNA FOXD3-AS1/miR-135a-5p function in nasopharyngeal carcinoma cells
- MicroRNA-182-5p relieves murine allergic rhinitis via TLR4/NF-κB pathway
