Startseite Response of potato tuber as an effect of the N-fertilizer and paclobutrazol application in medium altitude
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Response of potato tuber as an effect of the N-fertilizer and paclobutrazol application in medium altitude

  • Syariful Mubarok EMAIL logo , Anne Nuraini , Kusumiyati Kusumiyati , Nursuhud Suwali und Jajang Sauman Hamdani
Veröffentlicht/Copyright: 20. November 2024

Abstract

Development of G0 potato seeds in medium altitude with high temperatures has been constrained high temperatures, which inhibited plant growth and development, especially for tuber formation. The production of G0 potato seeds in the high-temperature area can be increased by the application of nitrogen (N) fertilizer and paclobutrazol (PBZ). The study aimed to identify the effect of different doses of N-fertilizer and the frequency of PBZ application on the plant growth and yield of first-generation potato seeds (G0) in medium land. The Randomized Complete Block Design with two factors was used in this experiment. The first factor was the dose of N-fertilizer (n 1 = 60, n 2 = 120, and n 3 = 180 kg/ha) and the second factor was the frequency of PBZ application (p 1 = 1, p 2 = 2, and p 3 = 3 times), applied starting at 40 DAP and repeated every 10 days with a concentration of 100 ppm. The results showed that there was a significant interaction between the dose of N-fertilizer and the frequency of PBZ application on plant dry weight. The 120 kg/ha of N-fertilizer-treated plants exhibited the highest plant height and the number of stolons per plant, whereas three times PBZ application treated plant exhibited the higher tuber growth rate, the number, and weight of tuber per plant with the value of number and weight of tuber per plant of 9.26 and 83.97 g, respectively. It can be concluded that N-fertilizer increased plant growth, whereas PBZ increased G0 potato seeds in the medium land altitude with high temperature.

1 Introduction

Potato (Solanum tuberosum L.) is one of the important vegetable crops in Indonesia. For increasing potato productivity and quality, the potato seed quality is an important factor that must be considered. In Indonesia, for an area of 72,000 ha, approximately 108 thousand tons of potato seeds are needed, while the availability of nationally certified potato seeds only reaches 15% of the total domestic potato seed needs [1]. In tropical countries such as Indonesia, potato seed production centers are in the highlands due to low temperatures.

However, it will be affected by negative environmental effects such as erosion and decreased soil productivity [2]. To increase potato seed production, cultivation in the medium-altitude area with a range of 400–700 m above sea level [3] is an alternative to increase potato seed production.

High temperature in the medium land inhibits tuber formation and decreases plant productivity [4]. To increase potato productivity, several strategies were followed such as the use of fertilizer and hormonal application. Despite various strategies to increase potato productivity, there is limited research on optimizing N-fertilizer levels in combination with paclobutrazol (PBZ) application under high-temperature conditions. N-fertilizer is the most needed mineral in cropping systems that plays an important role in the growth, yield, and quality of various crops [5,6,7]. Nitrogen (N) is one of the most important nutrients required for crop production for leaf area expansion and chlorophyll biosynthesis [8,9]. Biemond and Vos [10] stated that increasing N-fertilizer application enhanced the partitioning of assimilates to the shoots. The high doses of N-fertilizer could result in the inhibition of tuber formation [11]. Therefore, it is needed to investigate the optimum N-fertilizer that can affect the tuberization at medium altitude. However, fertilization management of nitrogen is very important, as the application and uptake of reduced forms of nitrogen such as urea, ammonium, and amino acids could improve water use efficiency of plants [12,13].

Inhibition in tuber formation is also influenced by the high temperature [14]. High temperature triggers the gibberellic acid or gibberellin (GA) biosynthesis that affects the tuber formation [15,16,17]. Therefore, to improve tuber formation, GA activity must be inhibited. PBZ is a GA inhibitor that is effective in inhibiting GA biosynthesis to suppress the growth and assimilate translocations of potato plants at the top and further increase the translocation of assimilating to the potato’s tuber [18]. The application of PBZ affects the decrease in plant height and leaf area index, and increase in chlorophyll content, net assimilation rate, wet weight of tuber, dry weight of tuber, yield per ha, and percentage of tuber grade quality [19,20,21,22,23]. Appropriate timing of PBZ application effectively inhibits the synthesis of GA that can focus on the initiation, formation, and enlargement of the tuber. The application of PBZ with different frequencies causes the effectiveness of the work of PBZ on different plants as well [24,25,26].

Potato plants have several growth phases. The application of PBZ was effectively carried out at the beginning of the tuber formation compared to being given at the end of the tuber formation. At the beginning of the formation of the tuber, assimilation is more directed to the tuber so that the resulting tuber is maximum [22]. Thus, this experiment aimed to obtain the most effective dose of N-fertilizer and the best frequency of PBZ on the growth and yield of G0 potato seeds at medium altitude.

2 Materials and methods

2.1 Plant preparation and experimental design

The potato seeds (G0 potato) from PT. Horti Agro Makro were grown at the Screen House, Ciparanje Jatinangor Experimental Garden, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang, Jawa Barat at an altitude of ±752 m above sea level, with longitude and latitude of 6.9203°S and 107.7727°E, respectively. This cultivation was conducted from March to June 2023 with an average temperature and humidity of 27 ± 4°C and 85 ± 5%, respectively. Randomized complete block design with two factors was used in this experiment. The first factor was the dose of N-fertilizer (n 1 = 60 kg/ha, n 2 = 120 kg/ha, and n 3 = 180 kg/ha of N), and the second factor was the frequency of PBZ application (p 1 = 1 time at 40 days after planting [DAP]); p 2 = 2 times at 40 and 50 DAP, and p 3 = 3 times at 40, 50, and 60 DAP. Each treatment consisted of ten polybags with one potato plant for each and repeated three times. The investigated plants were cultivated in polybags (20 cm × 20 cm) containing a combination of soil and compost (2:1/v:v) as the growing medium [27].

2.2 Fertilizer and PBZ application

During plant growth and development, the plants were watered twice daily in the morning and night with drip irrigation system. The fertilizers were applied by the soil drenching method at 2 and 4 weeks after planting (WAP). The doses of N-fertilizer were based on the treatment, whereas the doses of phosphorus and potassium were 150 kg/ha SP36 and 100 kg/ha KCl, respectively. The PBZ application was carried out with a concentration of 100 ppm at the appropriate time of treatment at a dose of 15 mL/plant at each time of treatment according to the method described by Hamdani et al. [21]. For pest and disease control during plant growth, 80% mancozeb and deltamethrin 2 g L−1 were sprayed to whole the plants. The plants were harvested at 85–90 DAP indicated by the yellowing stems and leaves [27].

2.3 Leaf area and leaf area index analysis

Leaf area and leaf area index measurements were carried out at 8 WAP using the gravimetric method as described by Jonckheere et al. [28]. Briefly, the leaf area was measured by correlating the dry weight of leaves and leaf area using predetermined green-leaf-area-to-dry-weight ratios, whereas the leaf area index, was measured by dividing leaf area per unit area of land [29].

2.4 Root dry weight, plant dry weight, and plant yield

Plants are harvested at the age of 90 DAP with the characteristics of the plant leaves having turned yellow and dry and the stems drying out. After harvesting, several yield variables were observed, namely plant dry weight (g) and root dry weight (g). At the harvest time, the plant yield was analyzed, namely the number of stolons, the percentage of stolons forming tubers per plant, and the number and weight of tubers per plant. The shoot and root ratio was measured at 8 WAP by dividing the dry biomass of roots with the dry biomass of the combination of stem, branches, and foliage.

2.5 Relative growth rate

Relative growth rate was calculated for the entire plant. The entire plant sample was taken destructively at 9, 10, and 11 WAP and then dried at a temperature of 70°C, until dry weight was constant. Relative growth rate values were calculated as the average dry weight values between the initial and final harvest as described by Nafi’ah et al. [30].

2.6 Tuber growth rate

The tuber growth rate was analyzed according to the method described by Rogi et al. [31] and Mailangkay et al. [32] with modification. Briefly, the samples were taken destructively at 9, 10, and 11 WAP. Then dried at a temperature of 70°C, until dry weight was constant. The growth rate from 9–10 to 10–11 WAP described the increase in the average dry weight of tubers per plant per day on average over 7 days.

2.7 N Nutrient uptake analysis

The uptake of N nutrients in potato plants was carried out at 9 WAP destructively by the entire plant and then dried at a temperature of 70°C. Nutrient uptake comprised N total (%) that was detected by the Kjeldahl method as described by Yunindanova et al. [33].

2.8 Statistical data analysis

The SPSS statistical program version 2.25 was used for data analysis. A two-factor analysis of variance was conducted to analyze the data, followed by Duncan’s multiple range test to compare differences among treatments. The difference was considered significant at the 5% level [34].

3 Results

3.1 Plant height, leaf area, leaf area index, shoot root ratio, and root dry weight

The statistical data analysis showed that there was no significant interaction between the dose of N-fertilizer and the frequency of PBZ application (p > 0.05) on plant height, leaf area, leaf area index, shoot root ratio, and root dry weight. Independently, the dose of N-fertilizer significantly affects (p < 0.05) plant height, leaf area, leaf area index, and dry weight roots. Meanwhile, the frequency of PBZ application has a significant effect (p < 0.05) on plant height, leaf area, and leaf area index (Table 2). The high doses of N-fertilizer 180 kg/ha significantly reduced the growth of potato plant by decreasing plant height, leaf area, and also leaf area index, but the application of lower N-fertilizer doses of 60 and 120 kg/ha resulted in a similar effect in plant height, leaf area, leaf area index, and dry weight roots. The application of two and three times of PBZ significantly affects the inhibition of plant growth by reducing plant height, leaf area, and leaf area index compared with one-time application (Table 1).

Table 1

Effect of N-fertilizer dose and the frequency of PBZ application on the N nutrient uptake

Treatment N nutrient uptake (%)
n1p1 5.53a
n2p1 4.97b
n3p1 4.34b
n1p2 4.46b
n2p2 4.69b
n3p2 5.36a
n1p3 4.45b
n2p3 4.19b
n3p3 5.65a

The mean values followed by the same letter were not significantly different based on Duncan’s Multiple Range test at the 5% level.

3.2 Plant dry weight

The results showed that there was a significant interaction between the dose of N- fertilizer and the frequency of PBZ application on the plant’s dry weight (p < 0.05) (Table 2). The interaction effects of N-fertilizer dose and PBZ application frequency were different among the treatments. The dose of 60 kg/ha (n 1) of N-fertilizer and the frequency of PBZ 1 time (p 1) resulted in the highest plant dry weight compared with other treatments, followed by 120 kg/ha with one- and three-times application. However, the increased N-fertilizer dose and the frequency of PBZ application significantly reduced the plant’s dry weight (Table 2).

Table 2

Effect of N-fertilizer dose and the frequency of PBZ application on plant height, leaf area, leaf area index, shoot-root ratio, and root dry weight

Treatment Plant height 8 WAP Leaf area (cm2) Leaf area index Shoot root ratio Root dry weight (g)
N fertilizer dose ( n )
n 1: 60 kg/ha 25.26ab 196.77b 0.164b 6.41a 2.13b
n 2: 120 kg/ha 26.37b 182.13b 0.151b 5.48a 2.24b
n 3: 180 kg/ha 24.19a 124.41a 0.103a 6.58a 1.64a
Frequency of PBZ application ( p )
p 1: 1 time (40 DAP) 25.96b 216.94b 0.181b 6.91a 2.10a
p 2: 2 times (40, 50 DAP) 25.86b 145.21a 0.121a 5.75a 1.90a
p 3: 3 times (40, 50, 60 DAP) 24.00a 140.95a 0.117a 5.82a 2.02a

The mean values followed by the same letter were not significantly different based on Duncan’s Multiple Range test at the 5% level.

3.3 Relative growth rate and tuber growth rate

The statistical data analysis showed that there was no interaction between the dose of N-fertilizer and the frequency of PBZ application (p > 0.05) on the relative growth rate and tuber growth rate. The individual effect of the dose of N-fertilizer and the frequency of PBZ application had no significantly different effects on the relative growth rate (Table 3). Meanwhile, only the individual effect of PBZ application frequency significantly affected the tuber growth rate at 10–11 WAP, where the application of PBZ three times resulted in the highest tuber growth rate at 10–11 WAP and was significantly different from other PBZ application frequencies (Table 3).

Table 3

Effect of N-fertilizer dose and the frequency of PBZ application on plant dry weight (g)

N-fertilizer dose (n) Frequency of PBZ application (p)
p 1 = (1 time) p 2 = (2 times) p 3 = (3 times)
n 1 = (60 kg/ha) 20.28b 12.85a 12.61a
C A A
n 2 = (120 kg/ha) 15.71a 12.33a 13.88a
B A B
n 3 = (180 kg/ha) 10.41a 13.13a 12.14a
A A A

The mean values followed by the same capital letter vertically and lower case horizontally were not significantly different based on Duncan’s Multiple Range test at the 5% level.

3.4 Number of stolons, percentage of stolons forming tubers per plant, and the number and weight of tubers per plant

The statistical analysis showed that there was no interaction between the dose of N-fertilizer and the frequency of PBZ application (p > 0.05) on the number of stolons, the percentage of stolons forming tubers per plant, the number of tubers, and the weight of tubers per plant. Independent effect of the dose of N-fertilizer significantly affects (p < 0.05) the number of stolons and the percentage of stolons forming tuber per plant but does not significantly affect the number and weight of tuber per plant. Meanwhile, the frequency of PBZ application has a significant effect (p < 0.05) on the number and weight of tuber per plant but no significant effect (p > 0.05) on the number of stolon and the percentage of stolon forming tuber per plant (Table 4). The application of N-fertilizer 120 kg/ha resulted in the highest number of stolons and was significantly different compared with other treatments, but resulted in the lowest percentage of stolon forming tuber with the value. The frequency of PBZ application three times produced the highest number and weight of tuber per plant and was significantly different compared with the other frequency PBZ applications with the value of 9.26 and 83.97 g, respectively (Table 4).

Table 4

Effect of N-fertilizer dose and the frequency of PBZ application on relative growth rate and tuber growth rate

Treatment Relative growth rate (g g−1 day−1) Tuber growth rate (g plant−1 day−1)
9–10 WAP 9–10 WAP 9–10 WAP 10–11 WAP
N-fertilizer dose ( n )
n 1: 60 kg/ha 0.1326a 3.7714a 3.7714a 4.7166a
n 2: 120 kg/ha 0.0870a 2.2441a 2.2441a 4.5727a
n 3: 180 kg/ha 0.1285a 1.2379a 1.2379a 3.1979a
Frequency of PBZ application ( p )
p 1: 1 time (40 DAP) 0.1192a 1.9067a 1.9067a 2.6041a
p 2: 2 times (40, 50 DAP) 0.1308a 2.5151a 2.5151a 2.5755a
p 3: 3 times (40, 50, 60 DAP) 0.0982a 0.0291a 2.8316a 7.3076b

The mean values followed by the same letter were not significantly different based on Duncan’s Multiple Range test at the 5% level.

3.5 N Nutrient uptake in plants

The statistical data analysis showed that the dose of N-fertilizer and the frequency of PBZ application significantly influenced the N absorption in potato plants (p < 0.05). The highest N absorption by potato plant was identified from n3p3 (180 kg/ha N with three times application) treated plants with the N nutrient uptake of 5.65%, but it was not significantly different from n1p1 (60 kg/ha N with 1 times application) and n3p2 (120 kg/ha N with two times application) (Table 5).

Table 5

Effect of the dose of N-fertilizer and the frequency of PBZ application on the number and percentage of stolons forming tuber per plant, the number of tubers, and weight of tuber per plant

Treatment Number of stolons Percentage of stolons forming tuber (%) Number of tubers per plant Weight of tuber per plant (g)
N-fertilizer dose ( n )
n 1: 60 kg/ha 10.67a 97.70b 7.33a 66.16a
n 2: 120 kg/ha 22.33b 51.25a 7.74a 63.09a
n 3: 180 kg/ha 11.33a 87.48ab 7.67a 67.75a
Frequency of PBZ application ( p )
p 1: 1 time (40 DAP) 15.89a 71.22a 6.96a 54.90a
p 2: 2 times (40, 50 DAP) 15.44a 76.99a 6.52a 58.13a
p 3: 3 times (40, 50, 60 DAP) 13.00a 88.21a 9.26b 83.97b

The mean values followed by the same letter were not significantly different based on Duncan’s multiple range test at the 5% level.

4 Discussion

High temperature affects potato production in the tropics [35]. This condition reduces potato productivity due to the failure of the tuberization process and the reduction of partitioning of net photosynthesis and assimilation [36]. The inhibited potato tuberization at the high temperature is mediated by the increasing biosynthesis of endogenous GA [37]. Therefore, PBZ is mostly used as a GA-biosynthesis inhibitor to promote tuber initiation [38].

In cropping systems, N fertilization plays an inevitable role in enhancing plant growth, yield, and quality [39,40]. PBZ led to increased absorption and transportation of nitrogen in plant by increasing the expression and activity of nitrogen transporters such as nitrate transporters in roots that are responsible for the uptake of nitrogen from the soil in the form of nitrate (NO3 ) or ammonium ( NH 4 + ). This improved activity leads to more effectiveness in nitrogen use for plant growth and development. Oliveira [41] stated that the availability and the N nutrient uptake have a positive effect on the growth of stems and leaves, increasing the plant height. This study reported that the application of N-fertilizer three times with the application of PBZ two and three times significantly increased N nutrient uptake (Table 5). Tsegaw [19] stated that the increased total N concentration in tubers from PBZ-treated plants may be due to increased uptake of N from the soil and/or remobilization of N. However, the higher N nutrient uptake by the potato plant affects the reduction of plant vegetative growth (Table 1). The mechanism of PBZ in increasing N assimilation by increasing the activity such as nitrate reductase and glutamine synthetase to convert absorbed nitrate into amino acids and proteins within the plant that can be used for growth and development. N enhanced assimilation partitioning to the shoots rather than to the tubers [42], therefore the leaf area was higher at the optimum doses (Table 1).

The optimum doses of N-fertilizer are important for plant growth by balancing plant growth between shoot and root, which finally supports the plant health. Low N-fertilizer prioritizes the root growth deeper over vegetative shoot growth to maximize nutrient capture, while high N-fertilizer triggers vegetative shoot growth that results in excessive lush foliage and also weaker stems, underdeveloped root system, and susceptibility to pests and diseases. Zelalem et al. [43] stated that the availability of nutrients in the growing media will support optimal plant growth such as growth in the leaf organ. The application of N affects the plant and root dry weight (Tables 1 and 2). A similar study was reported by Irawan and Kafiar [44] that N increases the accumulation of dry matter in the plant canopy and the growth of the plant canopy related to the root growth.

The frequency of PBZ application directly affects plant growth. Repeated application of PBZ causes sustained inhibition of GA biosynthesis, thereby maintaining suppression of GA production and preventing its recovery, keeping the plant in a dwarf. While a single application of PBZ may have a temporary effect. After the PBZ effect is metabolized, the plant will resume normal GA production and the plant will grow normally. PBZ is a growth inhibitor and also belongs to the triazol group [45]. Several studies reported that the effects of the chemical increased with increasing concentration, frequency, and time of application [46]. This study reported that the PBZ frequency application significantly reduced the plant height, leaf area, and leaf area index, where three times of application resulted in the lowest value (Table 1). Azima et al. [47] stated that the earlier application of PBZ reduced the leaf area and leaf area index. A similar result was reported by Mariana and Hamdani [48], the frequency of PBZ application two or three times was able to reduce the leaf area. PBZ inhibits GA biosynthesis resulting in slow division and elongation without causing toxicity to cells [49]. Hamdani et al. [24] reported that the three times PBZ application significantly reduced potato plant height. A similar study is reported by us (Table 1).

N-fertilizer at low doses increased the leaf area, leaf area index, and root and plant dry matter (Tables 1 and 2). They showed that increased leaf area resulted in an increase in plant dry weight. Sonbai et al. [50] stated that leaf area contributed to the plant’s dry matter by up to 70%. Van Keulen et al. [51] stated that N nutrients have an important role in the development of canopies and the accumulation of the plant’s dry weight. The application of PBZ reducing the GA level in the plant leaf to higher carbohydrate allocation to the root resulted in more dry matter allocation to the roots, as shown by Yim et al. [52].

PBZ-treated plants with three times application exhibited a higher tuber growth rate but did not show differences in plant growth rate (Table 2). A similar study was reported by Tsegaw [19] that the PBZ-treated plants exhibited a higher tuber growth rate and it would be influenced by leaf area. However, in our study, plants treated with three times PBZ application exhibited a higher tuber growth rate despite the reduced leaf area (Tables 1 and 3).

Stolon initiation and growth are stimulated by the high temperature and long photoperiod, but low temperature and short photoperiod initiate the tuberization and tuber expansion [53]. This study reported that N application at optimum doses under high-temperature areas increased the number of stolons and reduced the percentage of stolon forming tuber than at 60 or 180 kg/ha (Table 4). Although the function of N in potato tuberization is not well understood [15], N withdrawal affects the phytohormone balance in such a way that the level of GA decreases while increasing ABA levels [11]. Nitrogen application influences hormonal balances in plants, particularly the levels of cytokinins and GAs. GA promotes vegetative growth, including stolon elongation, while cytokinins support cell division and growth. High temperatures further disrupt this balance, favoring GA synthesis over cytokinin activity, which is crucial for tuber formation. With the application of PBZ, the GA activity is suppressed by the expression StSP6A and StGA2ox1.

The critical role in determining potato yield is the stolon-forming tuber and the number of tubers that are affected by the partitioning of carbon and N [54]. PBZ inhibits the synthesis of GAs that affects the reduction of cell elongation and vegetative growth. With the reduced vegetative growth, the carbon partitioning is shifting from the shoots to the roots and storage organs (tubers). Tubers are formed from the stolon swelling that is affected by several factors. Mares et al. [55] reported that GA inhibited tuber formation, decreased tuber sink strength, and encouraged shoot and stolon growth. Therefore, to initiate the tuber formation, the GA function must be minimized such as by the application of PBZ. Fletcher et al. [56] stated that PBZ increases the partitioning of assimilates to tubers thereby increasing yield. We have reported a similar study that the application of PBZ three times increased the number of tubers per plant and weight of tuber per plant (Table 4).

5 Conclusion

The application of N and PBZ significantly affects the plant growth and yield of potato seeds G0. The application of N-fertilizer 120 kg/ha resulted in a higher plant height and the number of stolons compared to 60 and 180 kg/ha treated plants but did not affect the number and weight of tubers. However, the increasing number and weight of tubers are affected by the PBZ application, where three times application resulted in a higher number and weight of tubers than one- and two-times application. We conclude that the application of N-fertilizer and PBZ can be useful for increasing G0 potato production and yield and is beneficial for increasing G0 potato seed productivity, especially for areas with high temperatures.

Acknowledgments

We thank the Universitas Padjadjaran for funding this research under ALG UNPAD research grant 2024. We also thank all members of our laboratory for helpful discussions throughout the work.

  1. Funding information: This research was funded by ALG UNPAD 2024 Research Grand No. 1476/UN6.3.1/PT.00/2024 and The APC was funded by Universitas Padjadjaran.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and consented to its submission to the journal, reviewed all the results and approved the final version of the manuscript. Conceptualization: SM, JSH; data curation SM, JSH; formal analysis: SM; funding acquisition: JHS; investigation: SM, SN, KK, JSH; methodology: SM, JSH; validation: SM, JSH; visualization: SM; writing – original draft: SM; writing – review & and editing: SM, AN, KK, NS, JSH.

  3. Conflict of interest: Authors state no conflicts of interest.

  4. Data availability statement: The authors confirm that the data supporting the findings of this study are available in the article.

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Received: 2024-03-28
Revised: 2024-09-13
Accepted: 2024-10-09
Published Online: 2024-11-20

© 2024 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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