Effect of the beta-adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses

Michael O. Allen; Toyin M. Salman; Abdul Rasak A. Alada; Adeyemi F. Odetayo; Eli B. Patrick; Shakiru A. Salami

doi:10.1515/jcim-2021-0062

Article

Effect of the beta-adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses

Michael O. Allen , Toyin M. Salman , Abdul Rasak A. Alada , Adeyemi F. Odetayo , Eli B. Patrick and Shakiru A. Salami

Published/Copyright: July 29, 2021

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From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 2

Abstract

Objectives

To investigate effect of beta adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses.

Methods

Experiments were carried out on 35 fasted male anaesthetized dogs weighing between 9 and 16 kg. The animals were divided into 7 (5 dogs per group) groups. Group I dogs served as control and infused with normal saline, groups II-IV were intravenously infused with glucose (1.1 mg/kg/min), fructose (1.1 mg/kg/min) and galactose (1.1 mg/kg/min) respectively while groups V-VII animals were pretreated with propranolol (0.5 mg/kg) and were infused with glucose, fructose or galactose respectively. A vein draining the proximal segment of the jejunum was cannulated along with right and left femoral arteries and veins. Glucose uptake was calculated as the product of jejunal blood flow and the difference between arterial and venous glucose levels (A-V glucose), part of the jejunum tissue was homogenized for estimation of glycogen concentration, and plasma lactate was assayed using lactate colorimetric kit.

Results

The result showed significant increase in venous lactate production in response to glucose (78.30 ± 4.57 mg/dL), fructose (60.72 ± 1.82 mg/dL) and galactose (71.70 ± 1.30 mg/dL) when compared with the control group (51.75 ± 1.32 mg/dL) at (p<0.05) with no significant difference in animals pretreated with propranolol. There was no significant difference in glycogen concentration (p>0.05) in animals infused with hexoses only compared with propanolol pretreated group.

Conclusions

Results suggests that one of the possible fates of the enormous amount of glucose taken up by the intestine is conversion to lactate and not glycogen and β-adrenergic receptor does not affect it.

Keywords: beta blockade; glucose uptake; lactate production

Corresponding author: Michael O. Allen, Department of physiology, Lagos State University College of Medicine, P.M.B 21266, Ikeja, Lagos, Nigeria, Phone: +2348137817631, E-mail: allenmike2000@gmail.com

Acknowledgments

I would like to express my very great appreciation to Dr Shittu of physiology department, University of Ibadan for his guidance and support during this research work. I would like to offer my special thanks to Late Mr Okon for his inmensed contributions. I am particularly grateful for the assistance given by the Biochemistry department University of Ibadan. Assistance provided by all was greatly appreciated.

Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: The protocols and procedures used in this study were approved by the Animal Ethics Committee of University of Ilorin College of Medicine and conform to the 1985 Guidelines for Laboratory Animal Care of the National Institute of Health.

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Received: 2021-03-02

Accepted: 2021-07-05

Published Online: 2021-07-29

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Articles in the same Issue

https://doi.org/10.1515/jcim-2021-0062

Keywords for this article

beta blockade; glucose uptake; lactate production