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Amelioration of related complications by the combined usage of Gymnadenia orchidis Lindl and pumpkin seed in type 2 diabetic mice

  • Subiah Helal Arzoo , Krishna Chattopadhyay , Tania Parvin and Brajadulal Chattopadhyay EMAIL logo
Published/Copyright: December 9, 2021
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 2

Abstract

Objectives

Inflammation, insulin resistance, hyperinsulinemia and cell damage are the major patho-physiological reasons behind type 2 diabetes (T2DM), which is one of the most prevalent non communicable metabolic disorders in the world. Oral hypoglycemic drugs and insulin shots are usually exercised to treat the diabetic patients but it produces many side effects. Thereby paving the way for natural hypoglycemic agents; a Himalayan herb and alternative nutritional therapy; low glycaemic indexed pumpkin seed, are used in combination for a better management of the disease. The aim of the study was to explore the combined efficacy of Gymnadenia orchidis Lindl root Salep and low-glycemic indexed-pumpkin seeds in better management of T2DM and associated complications.

Methods

Balb/c mice were randomly allocated to six different groups (n=5). Streptozotocin along with high-fat-diet was used to induce T2DM. The experimental animals were supplemented with low-glycemic food or root Salep (200 mg/kg body weight) or combination of both according to their groups for 21 days, post which various biochemical tests were performed.

Results

T2DM augmented the IL-6, IFN-γ, TNF-α, BAX, Insulin levels, and HOMA-IR with concurrent reduction of IL-4, QUICKI, Bcl-2, estradiol and progesterone levels. FACS revealed augmented cellular damage in T2DM mice. Interestingly, root Salep and pumpkin seeds normalized those parameters in T2DM animals suggesting significant (p<0.001) improvement of immunity of the diseased animals and ameliorated associated complications.

Conclusions

Root Salep and pumpkin seed display synergism among binomial set of herbal agents which may be safely used for T2DM management.

Keywords: herbal agent; insulin resistance; pumpkin seed; root Salep; type 2 diabetes
Corresponding author: Dr. Brjadulal Chattopadhyhay, Department of Physics, Jadavpur University, Kolkata 700032, West Bengal, India, Phone: +91 9433343917, E-mail:

Funding source: Research Society for the Studies of Diabetes in India and fellowship Grant from UGC, Government of India

Award Identifier / Grant number: (UGC-Ref. No.: 1107/(OBC) (NET-July 2016).

Acknowledgments

The financial support by the Research Society for the Studies of Diabetes in India is gratefully acknowledged. Miss Subiah H. Arzoo gratefully acknowledges the fellowship from UGC, Government of India (UGC-Ref. No.: 1107/(OBC) (NET-July 2016). The authors are thankful to the Biophysical Laboratory of Physics Department, Jadavpur University for providing experimental facilities and Dr. Bholanath Paul, Ex Scientist, CDRI, Lucknow for technical help.

  1. Research funding: This research was funded by Research Society for the Studies of Diabetes in India.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The work was approved by the Animal Ethics Committee of Jadavpur University, Kolkata, India (Ref. No.: AEC/PHARM/ 1601/11/ 2016).

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Received: 2021-10-28
Accepted: 2021-11-29
Published Online: 2021-12-09

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2021-0499
Keywords for this article
herbal agent; insulin resistance; pumpkin seed; root Salep; type 2 diabetes

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Swarna Prashana – faith in the embrace of research
  4. A review on the concept of aging in Ayurveda literature
  5. Ethno-pharmacology of Asaroon (Asarum europaeum L.) with special reference to Unani System of Medicine
  6. Is Trikatu an ayurvedic formulation effective for the management of flu-like illness? A narrative review
  7. The anti-cancer properties of neem (Azadirachta indica) through its antioxidant activity in the liver: its pharmaceutics and toxic dosage forms. A literature review
  8. The role of acupuncture for myofascial pain syndrome (MPS) in interventional pain management
  9. Research Articles
  10. Identification of phenolic compounds in the buds of Algerian Pistacia atlantica desf. Subsp. atlantica by antioxidant activity guided fractionation
  11. Optimization of water-based ultrasonic-microwave assisted extraction (UMAE) of bioactive compounds from Garcinia mangostana pericarp
  12. Proximate analysis, HPTLC finger print analysis and multi spectrometric analysis of Strychnos nux-vomica nuts
  13. Amelioration of sodium fluoride induced oxidative stress by Cynometra travancorica Bedd in mice
  14. The effects of saffron petals and damask rose petals on biochemical and inflammatory measurements
  15. Ethanolic extract of Achillea wilhelmsii C. Koch improves pulmonary function and inflammation in LPS-induced acute lung injury mice
  16. Interaction between properties of commercial Australian honey and saliva: in vitro exploratory study of a potential mechanism in the treatment of oral mucositis
  17. The potential of immature poken (Citrus reticulata) extract in the weight management, lipid and glucose metabolism
  18. Effect of the beta-adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses
  19. Investigating the effect of the Teucrium polium aqueous extract on the liver of the streptozotocin-induced diabetic rats
  20. Synergistic effects of high-intensity interval training (HIIT) and intake of nettle on serum levels of chemerin and beta cells function in type 2 diabetic rats
  21. The effect of Cinnamomum cassia extract on oxidative stress in the liver and kidney of STZ-induced diabetic rats
  22. Protective role of Sterculia tragacantha aqueous extract on pancreatic gene expression and oxidative stress parameters in streptozotocin-induced diabetic rats
  23. Ameliorating activity of polyphenolic-rich extracts of Basella rubra L. leaves on pancreatic β-cell dysfunction in streptozotocin-induced diabetic rats
  24. Amelioration of related complications by the combined usage of Gymnadenia orchidis Lindl and pumpkin seed in type 2 diabetic mice
  25. The effect of Madhumeha Kusumakar Rasa – an Ayurved medicine – in insulin resistance
  26. Anti-obesity effects of the n-butanol fraction of the methanolic leaf extract of Artemisia campestris from Tunisian pharmacopeia in male Wistar rats
  27. Compensatory depression of arterial pressure and reversal of ECG abnormalities by Annona muricata and Curcuma longa in hypertensive Wistar rats
  28. Preventing SARS-CoV-2 transmission in the emergency department by implementing a separate pathway for patients with respiratory conditions
  29. Clinical significance of serum gonadotropin and androgen levels among Egyptian overweight/obese pubertal girls
  30. Preference between serum homocysteine and urinary periostin as early predictive biomarkers of renal dysfunction among uncontrolled diabetics
  31. Mizaj assessment in multiple sclerosis patients based on Persian Medicine
  32. Immediate effects of the practise of Sheethali pranayama on heart rate and blood pressure parameters in healthy volunteers
  33. Effects of 12 sessions of steam bath intervention on spirometry parameters among healthy adult volunteers
  34. The effects of psychological counseling and acupressure based on couple therapy procedure for alleviation of vomiting and nausea in pregnant women in Iran country
  35. “The immediate effect of neutral spinal compress on heart rate variability in hypertensive individuals”
  36. Effect of whole-body vibration after a resistance exercise bout on heart rate variability in hypertensive population
  37. Wet-cupping on calf muscles in polycystic ovary syndrome: a quasi-experimental study
  38. Auricular field nerve stimulation using the NSS-2 BRIDGE® device as an alternative to opioids following kidney donor surgery
  39. Evaluation of general body temperament and uterine dystemperament in amenorrhoea: a cross-sectional analytical study
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  44. Case Reports
  45. Management of psoriasis with Ayurveda Panchakarma and Manibhadragula as Shodhana Rasayana – a case report
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  48. Tongue stretching: technique and clinical proposal
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