Amelioration of sodium fluoride induced oxidative stress by Cynometra travancorica Bedd in mice

Nair Meera; Menon K. Divya; Prabha Silpa; Chennattu M. Pareeth; Achuthan C. Raghavamenon; Thekkekara D. Babu

doi:10.1515/jcim-2021-0120

Article

Amelioration of sodium fluoride induced oxidative stress by Cynometra travancorica Bedd in mice

Nair Meera , Menon K. Divya , Prabha Silpa , Chennattu M. Pareeth , Achuthan C. Raghavamenon and Thekkekara D. Babu

Published/Copyright: November 19, 2021

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From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 2

Abstract

Objectives

Cynometra travancorica, endemic to Western Ghats of India is pharmacologically similar to Saraca asoca and occasionally used as substitute in a well-known Ayurvedic uterine tonic Asokarishta. S. asoca possess various biological properties, but there are no reports on C. travancorica. The present study evaluated the pharmacological properties of C. travancorica and its efficacy in attenuating the sodium fluoride (NaF) induced oxidative stress in mice.

Methods

Antioxidant potential of methanolic extract of C. travancorica (CTE) stem bark was evaluated using DPPH, superoxide radical scavenging and total antioxidant assays. The effect of CTE on mitigating NaF deteriorated redox status in the liver tissue of mice was evaluated. Functional groups in CTE were analyzed by FTIR analysis.

Results

CTE effectively scavenged the free radicals in in vitro condition. CTE could augment catalase (46.6%), superoxide dismutase (53.8%) activities and GSH level (48.1%) against NaF induced decline in the liver tissue of mice. The peroxidation of lipids was found to be decreased by 44.9% and tissue damage abated as inferred by histopathology. FTIR analysis revealed the presence of biologically active functional groups in CTE.

Conclusions

The study revealed the ameliorative effect of C. travancorica against NaF induced deleterious effect in experimental animals by its potent antioxidant potential.

Keywords: antioxidant; Cynometra travancorica ; oxidative stress; sodium fluoride

Corresponding author: Dr. Thekkekara D. Babu, Associate Professor, Department of Biochemistry, Amala Cancer Research Centre, Thrissur 680 555, Kerala, India, E-mail: babutharakan@gmail.com

Funding source: KSCSTE (Kerala State Council for Science, Technology and Environment)

Award Identifier / Grant number: KSCSTE No. 012-10/FSHP/2012/CSTE Dated 28.12.2012

Acknowledgments

The authors acknowledge the Department of Biochemistry, Amala Cancer Research Centre, Thrissur, Kerala for providing all the facilities to conduct the research work. The authors are thankful to KSCSTE (Kerala State Council for Science, Technology and Environment) for providing the research fellowship to undertake this work (No. 012-10/FSHP/2012/CSTE dated 28.12.2012).

Research funding: The authors are thankful to KSCSTE (Kerala State Council for Science, Technology and Environment) for providing the research fellowship to undertake this work (No. 012-10/FSHP/2012/CSTE dated 28.12.2012).
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest
Informed consent: Not applicable.
Ethical approval: The animal experiments accomplished in this study were carried out with the prior approval of the Institutional Animal Ethics Committee (IAEC) (Approval No. ACRC/IAEC/16-06/10 dated 20.06.16) and conducted strictly according to the guidelines of Committee for the purpose of Control and Supervision of Experiments on Animals (CPCSEA) constituted by the Animal Welfare Division, Government of India.

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Received: 2021-03-17

Accepted: 2021-11-03

Published Online: 2021-11-19

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Articles in the same Issue

https://doi.org/10.1515/jcim-2021-0120

Keywords for this article

antioxidant; Cynometra travancorica; oxidative stress; sodium fluoride