Home Medicine The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury
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The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

  • Deniz Ozturk , Derya Guzel Erdogan ORCID logo EMAIL logo , Ayhan Tanyeli , Selim Çomaklı , Hüseyin Baylan and Elif Polat
Published/Copyright: August 30, 2019

Abstract

Objective

The effects of urapidil in intestinal ischemia-reperfusion (IR) model were investigated using histopathological and biochemical methods.

Materials and methods

Forty Wistar albino rats were subjected to sham operation (Group 1), IR (Group 2), IR+dimethyl sulfoxide (Group 3), IR+urapidil 0.5 mg/kg (Group 4), and IR+urapidil 5 mg/kg (Group 5). Levels of MDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3B were measured.

Results and discussion

The groups 2 and 3 had significantly higher TOS and MPO levels than the sham group had (p < 0.001), whereas the TAS and SOD levels were significantly lower in Group 2 than in the sham group. In treatment groups, TAS and SOD levels increased, whereas TOS, MPO, and MDA levels decreased compared to Group 2. Caspase-3 and LC3B immunopositivities were seen at severe levels in Group 2 and 3. However, Group 4 and 5 were found to have lower levels of immunopositivity. Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2 and 3.

Conclusion

Our results show that urapidil is one of the antioxidant agents and protects lung tissue from oxidant effects of intestinal IR injury.

Öz

Amaç

İntestinal iskemi-reperfüzyon (IR) modelinde urapidil’in etkileri histopatolojik ve biyokimyasal yöntemler kullanılarak araştırıldı.

Gereç ve Yöntem

Kırk adet Wistar albino sıçan sham operasyon (Grup 1), IR (Grup 2), IR+dimetil sülfoksit (Grup 3), IR+urapidil 0.5 mg/kg (Grup 4) ve IR+urapidil 5 mg/kg (Grup 5) olarak gruplandı. MDA, TAS, TOS, SOD, MPO, NF-κB, kaspaz-3 ve LC3B seviyeleri ölçüldü.

Bulgular ve tartışma

Grup 2 ve 3’te sham grubuna göre TOS ve MPO düzeylerinin anlamlı derecede yüksek, (p < 0.001), TAS ve SOD düzeyleri grup 2’de sham grubundan anlamlı derecede düşüktü. Tedavi gruplarında TAS ve SOD seviyeleri yükselirken, TOS, MPO ve MDA düzeyleri Grup 2’ye göre arttı. Kaspaz-3 ve LC3B immünopozitivitesi Grup 2 ve 3’te yoğun seviyelerde görüldü İnterstisyel bölgelerde, peribronşiyal bölgede ve bronşiyal epitel hücrelerinde immünopozitivite gözlendi. Grup 2 ve 3’te orta düzeyde bir NF-kappa B immünopozitivitesi görüldü.

Sonuç

Sonuçlarımız urapidil’in, antioksidan ajanlardan biri olduğu ve akciğer dokusunu bağırsak IR hasarının oksidan etkilerinden koruduğunu göstermiştir.


Corresponding author: Derya Guzel Erdogan, MD, Asst. Prof., Sakarya University, Faculty of Medicine, Department of Physiology, No: 81/1, Korucuk Campus, Adapazarı/Sakarya, Turkey, Phone: +90 (264) 295 66 30, Fax: +0 (264) 295 66 29

Acknowledgements

There was no financial support organization in the implementation of the study. We would like to thank all participants for contributing in the present survey. Our study was presented as an oral presentation in ERPA International Congresses on Education, 2018.

  1. Conflict of interest: The authors declare no conflict of interest.

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Received: 2018-07-17
Accepted: 2019-03-04
Published Online: 2019-08-30

©2019 Walter de Gruyter GmbH, Berlin/Boston

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