Startseite Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
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Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor

  • Jana Šefčovičová , Jaroslav Filip und Jan Tkac EMAIL logo
Veröffentlicht/Copyright: 28. November 2014
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Abstract

Various types of carbon nanoparticles were directly mixed with microbial cells of Gluconobacter oxydans within a 3-D bionanocomposite in order to prepare a highly sensitive ethanol biosensor with a short response time. From all carbonaceous nanomaterials tested, single- or multi-walled carbon nanotubes provided the highest sensitivity of detection (117-121 μA cm−2 mM−1), but from a practical point of view, Ketjen black 300 and 600 provide very low detection limit (2-6 μM) and high sensitivity for the ethanol analysis (84-88 μA cm−2 mM−1) with a short response time (14-33 s). Moreover, the price of Ketjen black is a few orders of magnitude lower compared to that of carbon nanotubes. Finally, the study showed that the morphology of nanoparticles rather than their surface modification is the key element in achieving high sensitivity of ethanol detection.

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Received: 2014-3-25
Revised: 2014-5-22
Accepted: 2014-5-26
Published Online: 2014-11-28
Published in Print: 2015-1-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

Artikel in diesem Heft

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  2. Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
  3. Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
  4. Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
  5. Application of nanomaterials in microbial-cell biosensor constructions
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