Abstract
Various types of carbon nanoparticles were directly mixed with microbial cells of Gluconobacter oxydans within a 3-D bionanocomposite in order to prepare a highly sensitive ethanol biosensor with a short response time. From all carbonaceous nanomaterials tested, single- or multi-walled carbon nanotubes provided the highest sensitivity of detection (117-121 μA cm−2 mM−1), but from a practical point of view, Ketjen black 300 and 600 provide very low detection limit (2-6 μM) and high sensitivity for the ethanol analysis (84-88 μA cm−2 mM−1) with a short response time (14-33 s). Moreover, the price of Ketjen black is a few orders of magnitude lower compared to that of carbon nanotubes. Finally, the study showed that the morphology of nanoparticles rather than their surface modification is the key element in achieving high sensitivity of ethanol detection.
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© 2015 Institute of Chemistry, Slovak Academy of Sciences
Artikel in diesem Heft
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- Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
- Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
- Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
- Application of nanomaterials in microbial-cell biosensor constructions
- Use of green fluorescent proteins for in vitro biosensing
- Biosensors based on molecular beacons
- DNA aptamer-based detection of prostate cancer
- Can glycoprofiling be helpful in detecting prostate cancer?
- Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
- Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
- Surface plasmon resonance application in prostate cancer biomarker research
- Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
- Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
- Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
- Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
- Whole-cell optical biosensor for mercury – operational conditions in saline water
- Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
- Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
- Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
- Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
- Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
- Can voltammetry distinguish glycan isomers?
Artikel in diesem Heft
- Biosensors – Topical issue
- Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
- Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
- Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
- Application of nanomaterials in microbial-cell biosensor constructions
- Use of green fluorescent proteins for in vitro biosensing
- Biosensors based on molecular beacons
- DNA aptamer-based detection of prostate cancer
- Can glycoprofiling be helpful in detecting prostate cancer?
- Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
- Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
- Surface plasmon resonance application in prostate cancer biomarker research
- Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
- Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
- Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
- Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
- Whole-cell optical biosensor for mercury – operational conditions in saline water
- Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
- Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
- Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
- Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
- Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
- Can voltammetry distinguish glycan isomers?