Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
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Sophia Karastogianni
Abstract
A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the detection of the hepatitis B virus (HBV) using a manganese(II) complex as electrochemical indicator and a DNA probe-modified carbon paste electrode as the biosensor (DNA/CPE). The results showed that this complex could be accumulated electrochemically the immobilised dsDNA layer rather than in the single-stranded DNA (ssDNA) layer. On the basis of this, the manganese complex was used as an electrochemical hybridisation indicator for the detection of oligonucleotides related to HBV. The hybridisation event was evaluated on the basis of the difference between the reduction signals of the manganese(II) complex with the probe DNA prior to and post hybridisation with a target sequence using a differential pulse mode. Several factors affecting the immobilisation and hybridisation of oligonucleotides as well as the indicator’s accumulation were investigated. Experiments with a noncomplementary and mismatch sequences demonstrated the good selectivity of the biosensor. Using this approach, the HBV target oligonucleotide’s sequence could be quantified over arange from 0.22 ng L−1 to 5.40 ng L−1, with a linear correlation coefficient of 0.9994 and the limit of detection of 0.07 ng L−1.
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- Biosensors – Topical issue
- Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
- Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
- Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
- Application of nanomaterials in microbial-cell biosensor constructions
- Use of green fluorescent proteins for in vitro biosensing
- Biosensors based on molecular beacons
- DNA aptamer-based detection of prostate cancer
- Can glycoprofiling be helpful in detecting prostate cancer?
- Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
- Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
- Surface plasmon resonance application in prostate cancer biomarker research
- Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
- Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
- Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
- Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
- Whole-cell optical biosensor for mercury – operational conditions in saline water
- Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
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