Startseite Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
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Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization

  • Vedran Milosavljevic , Hoai Viet Nguyen , Petr Michalek , Amitava Moulick , Pavel Kopel , Rene Kizek und Vojtech Adam EMAIL logo
Veröffentlicht/Copyright: 28. November 2014
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 69 Heft 1

Abstract

Nanoparticles as a progressively developing branch offer a tool for studying the interaction of carbon quantum dots (CQDs) with DNA. In this study, fluorescent CQDs were synthesized using citric acid covered with polyethylene glycol (PEG) as the source of carbon precursors. Furthermore, interactions between CQDs and DNA (double-stranded DNA and single-stranded DNA) were investigated by spectral methods, gel electrophoresis, and electrochemical analysis. Primarily, the fluorescent behavior of CQDs in the presence of DNA was monitored and major differences in the interaction of CQDs with tested single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) were observed at different amounts of CQDs (μg mL−1: 25, 50, 100, 250, 500). It was found that the interaction of ssDNA with CQDs had no significant influence on the CQDs fluorescence intensity measured at the excitation wavelengths of 280 nm, 350 nm, and 400 nm. However, in the presence of dsDNA, the fluorescence intensity of CQDs was significantly increased. Our results provide basic understanding of the interaction between CQDs and DNA. Such fabricated CQDs-DNA might be of great benefit for the emerging nanomaterials based biosensing methods.

Keywords: carbon quantum dots; DNA; spectrophotometry; gel electrophoresis; electrochemical analysis

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Received: 2014-1-7
Revised: 2014-3-17
Accepted: 2014-3-18
Published Online: 2014-11-28
Published in Print: 2015-1-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

Artikel in diesem Heft

  1. Biosensors – Topical issue
  2. Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
  3. Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
  4. Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
  5. Application of nanomaterials in microbial-cell biosensor constructions
  6. Use of green fluorescent proteins for in vitro biosensing
  7. Biosensors based on molecular beacons
  8. DNA aptamer-based detection of prostate cancer
  9. Can glycoprofiling be helpful in detecting prostate cancer?
  10. Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
  11. Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
  12. Surface plasmon resonance application in prostate cancer biomarker research
  13. Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
  14. Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
  15. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
  16. Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
  17. Whole-cell optical biosensor for mercury – operational conditions in saline water
  18. Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
  19. Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
  20. Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
  21. Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
  22. Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
  23. Can voltammetry distinguish glycan isomers?
https://doi.org/10.2478/s11696-014-0590-2
Schlagwörter für diesen Artikel
carbon quantum dots; DNA; spectrophotometry; gel electrophoresis; electrochemical analysis

Artikel in diesem Heft

  1. Biosensors – Topical issue
  2. Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
  3. Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
  4. Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
  5. Application of nanomaterials in microbial-cell biosensor constructions
  6. Use of green fluorescent proteins for in vitro biosensing
  7. Biosensors based on molecular beacons
  8. DNA aptamer-based detection of prostate cancer
  9. Can glycoprofiling be helpful in detecting prostate cancer?
  10. Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
  11. Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
  12. Surface plasmon resonance application in prostate cancer biomarker research
  13. Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
  14. Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
  15. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
  16. Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
  17. Whole-cell optical biosensor for mercury – operational conditions in saline water
  18. Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
  19. Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
  20. Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
  21. Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
  22. Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
  23. Can voltammetry distinguish glycan isomers?
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