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Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds

  • Miroslav Pohanka EMAIL logo
Published/Copyright: November 28, 2014
Become an author with De Gruyter Brill

Abstract

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes expressed in the human body under physiological conditions. AChE is an important part of the cholinergic nerves where it hydrolyses neurotransmitter acetylcholine. Both cholinesterases are sensitive to inhibitors acting as neurotoxic compounds. In analytical applications, the enzymes can serve as a biorecognition element in biosensors as well as simple disposable sensors (dipsticks) and be used for assaying the neurotoxic compounds. In the present review, the mechanism of AChE and BChE inhibition by disparate compounds is explained and methods for assaying the enzymes activity are shown. Optical, electrochemical, and piezoelectric biosensors are described. Attention is also given to the application of sol-gel techniques and quantum dots in the biosensors’ construction. Examples of the biosensors are provided and the pros and cons are discussed.

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Received: 2013-11-3
Revised: 2013-12-12
Accepted: 2013-12-14
Published Online: 2014-11-28
Published in Print: 2015-1-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

Articles in the same Issue

  1. Biosensors – Topical issue
  2. Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
  3. Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
  4. Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
  5. Application of nanomaterials in microbial-cell biosensor constructions
  6. Use of green fluorescent proteins for in vitro biosensing
  7. Biosensors based on molecular beacons
  8. DNA aptamer-based detection of prostate cancer
  9. Can glycoprofiling be helpful in detecting prostate cancer?
  10. Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
  11. Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
  12. Surface plasmon resonance application in prostate cancer biomarker research
  13. Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
  14. Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
  15. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
  16. Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
  17. Whole-cell optical biosensor for mercury – operational conditions in saline water
  18. Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
  19. Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
  20. Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
  21. Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
  22. Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
  23. Can voltammetry distinguish glycan isomers?
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