Can voltammetry distinguish glycan isomers?
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Mojmír Trefulka
Abstract
The analysis of glycan isomers is an important task in glycomics. This study reveals for the first time that two oligosaccharide pentamers can be distinguished electrochemically. 3α,6α-mannopentaose (MPO) and verbascose (VBS) were modified using Os(VI)-temed and the carbohydrate adducts measured directly in the reaction mixture using cyclic voltammetric (CV) and square wave voltammetric (SWV) stripping at the hanging mercury drop electrode. Some CV and SWV responses of MPO were found to differ from those of VBS at full electrode coverage, requiring μM concentrations at short accumulation time-intervals. Similar results were obtained at partial electrode coverage at nM concentrations. These results afford an opportunity for the development of a new simple and inexpensive glycan analysis.
References
Alley, W. R., Jr., Mann, B. F., & Novotny, M. V. (2013). Highsensitivity analytical approaches for the structural characterization of glycoproteins. Chemical Reviews, 113, 2668-2732. DOI: 10.1012/cr3003714.Suche in Google Scholar
Bartosik, M., Trefulka, M., Hrstka, R., Vojtesek, B., & Palecek, E. (2013). Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers. Electrochemistry Communications, 33, 55-58. DOI: 10.1016/j.elecom.2013.04.009.10.1016/j.elecom.2013.04.009Suche in Google Scholar
Bertók, T., Katrlik, J., Gemeiner, P., & Tkac, J. (2013). Electrochemical lectin based biosensors as a label-free tool in glycomics. Microchimica Acta, 180, 1-13. DOI: 10.1007/s00604-012-0876-4. 10.1007/s00604-012-0876-4Suche in Google Scholar PubMed PubMed Central
Gangola, M. P., Jaiswal, S., Khedikar, Y. P., & Chibbar, R. N. (2014). A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC-PAD and its comparison with HPLC-RI. Food Chemistry, 154, 127-133. DOI: 10.1016/j.foodchem.2013.12.085. 10.1016/j.foodchem.2013.12.085Suche in Google Scholar PubMed
Heyrovský, J., & Babička, J. (1930). Polarographic studies with dropping mercury cathode. Part XIII. Effect of albumins. Collection of Czechoslovak Chemical Communications, 2, 370-378.10.1135/cccc19300370Suche in Google Scholar
Lindhorst, T. K. (2007). Essentials of carbohydrate chemistry and biochemistry. Weinheim, Germany: Wiley-VCH. Suche in Google Scholar
Novotny, M. V., Alley, W. R., Jr., & Mann, B. F. (2013). Analytical glycobiology at high sensitivity: current approaches and directions. Glycoconjugate Journal, 30, 89-117. DOI: 10.1007/s10719-012-9444-8.10.1007/s10719-012-9444-8Suche in Google Scholar PubMed PubMed Central
Obendorf, R. L., & Gorecki, R. J. (2012). Soluble carbohydrates in legume seeds. Seed Science Research, 22, 219-242. DOI: 10.1017/s0960258512000104.10.1017/S0960258512000104Suche in Google Scholar
Pabst, M., & Altmann, F. (2011). Glycan analysis by modern instrumental methods. Proteomics, 11, 631-643. DOI: 10.1002/pmic.201000517.10.1002/pmic.201000517Suche in Google Scholar PubMed
Paleček, E. (1960). Oscillographic polarography of highly polymerized deoxyribonucleic acid. Nature, 188, 656-657. DOI: 10.1038/188656a0.10.1038/188656a0Suche in Google Scholar PubMed
Paleček, E., & Trefulka, M. (2011). Electrocatalytic detection of polysaccharides at picomolar concentrations. Analyst, 136, 321-326. DOI: 10.1039/c0an00681e.10.1039/C0AN00681ESuche in Google Scholar PubMed
Paleček, E., & Bartošik, M. (2012). Electrochemistry of nucleic acids. Chemical Reviews, 112, 3427-3481. DOI: 10.1021/cr200303p.10.1021/cr200303pSuche in Google Scholar PubMed
Paleček, E., Bartošik, M., Ostatna, V., & Trefulka, M. (2012). Electrocatalysis in proteins, nucleic acids and carbohydrates. The Chemical Record, 12, 27-45. DOI: 10.1002/tcr.20110 0029.10.1002/tcr.20110Suche in Google Scholar PubMed
Paleček, E., & Řimankova, L. (2014). Chitosan catalyzes hydrogen evolution at mercury electrodes. Electrochemistry Communication, 44, 59-62. DOI: 10.1016/j.elecom.2014.04.015.10.1016/j.elecom.2014.04.015Suche in Google Scholar
Ruhaak, L. R., Zauner, G., Huhn, C., Bruggink, C., Deelder, A. M., & Wuhrer, M. (2010). Glycan labeling strategies and their use in identification and quantification. Analytical & Bioanalytical Chemistry, 397, 3457-3481. DOI: 10.1007/s00216-010-3532-z.10.1007/s00216-010-3532-zSuche in Google Scholar PubMed PubMed Central
Trefulka, M., Ostatna, V., Havran, L., Fojta, M., & Paleček, E. (2007). Covalent labeling of nucleosides with VIII- a VIvalent osmium complexes. Electroanalysis, 19, 1281-1287. DOI: 10.1002/elan.200703848.10.1002/elan.200703848Suche in Google Scholar
Trefulka, M., & Paleček, E. (2009). Voltammetry of Os(VI)- modified polysaccharides at carbon electrodes. Electroanalysis, 21, 1763-1766. DOI: 10.1002/elan.200904658.10.1002/elan.200904658Suche in Google Scholar
Trefulka, M., Bartošik, M., & Paleček, E. (2010). Facile end-labeling of RNA with electroactive Os(VI) complexes.Electrochemistry Communications, 12, 1760-1763. DOI: 10.1016/j.elecom.2010.10.016.10.1016/j.elecom.2010.10.016Suche in Google Scholar
Trefulka, M., & Paleček, E. (2010). Voltammetry of Os(VI)- modified polysaccharides. Electroanalysis, 22, 1837-1845. DOI: 10.1002/elan.201000218.10.1002/elan.201000218Suche in Google Scholar
Trefulka, M., & Paleček, E. (2012). Voltammetric determination of Os(VI)-modified oligosaccharides at nanomolar level. Bioelectrochemistry, 88, 8-14. DOI: 10.1016/j.bioelechem.2012. 04.005.Suche in Google Scholar
Trefulka, M., & Paleček, E. (2013). Modification of poly- and oligosaccharides with Os(VI)pyridine. Voltammetry of the Os(VI) adducts obtained by ligand exchange. Electroanalysis, 25, 1813-1817. DOI: 10.1002/elan.201300191. 10.1002/elan.201300191Suche in Google Scholar
© 2015 Institute of Chemistry, Slovak Academy of Sciences
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- Biosensors – Topical issue
- Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
- Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
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- Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
- Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
- Surface plasmon resonance application in prostate cancer biomarker research
- Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
- Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
- Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
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- Can voltammetry distinguish glycan isomers?
