Startseite Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
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Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye

  • Hui Xu , Robert Wallace und Maria Hepel EMAIL logo
Veröffentlicht/Copyright: 28. November 2014
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 69 Heft 1

Abstract

Albumin, as an antifouling agent preventing non-specific adsorption, is widely applied in biosensor construction and in the design of nanocarriers for theranostic applications. However, albumin is not completely inert and it interacts with many endogenous and exogenous compounds. The present work investigates the interactions of bovine serum albumin (BSA) with phenol red dye (PR), a common component of cell-growth media. PR was found to bind to BSA, with the affinity constant KA = (2.5 ± 0.8) × 105 L mol−1, and that it quenches the intrinsic BSA fluorescence through the F¨orster non-radiative resonance energy transfer (FRET) via static modes, with critical FRET distance of R0 = 2.65 nm. Synchronous fluorescence spectroscopy measurements indicated a conformational transition of BSA in the presence of PR to a conformation with tyrosine residues surrounded by a more hydrophobic environment. Thermodynamic parameters, ΔH°, ΔG° and ΔS°, determined using fluorescence and isothermal titration microcalorimetry, indicated that entropic contribution played a major role at ambient temperature. It was also found that common metal ions increased KA but Ca2+ and Fe3+ decreased it.

Keywords: bovine serum albumin; BSA-phenol red binding; fluorescence energy transfer; BSA conformation change; thermodynamic properties; isothermal titration calorimetry

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Received: 2014-4-21
Revised: 2014-6-7
Accepted: 2014-6-10
Published Online: 2014-11-28
Published in Print: 2015-1-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2015-0015
Schlagwörter für diesen Artikel
bovine serum albumin; BSA-phenol red binding; fluorescence energy transfer; BSA conformation change; thermodynamic properties; isothermal titration calorimetry

Artikel in diesem Heft

  1. Biosensors – Topical issue
  2. Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
  3. Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
  4. Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
  5. Application of nanomaterials in microbial-cell biosensor constructions
  6. Use of green fluorescent proteins for in vitro biosensing
  7. Biosensors based on molecular beacons
  8. DNA aptamer-based detection of prostate cancer
  9. Can glycoprofiling be helpful in detecting prostate cancer?
  10. Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
  11. Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
  12. Surface plasmon resonance application in prostate cancer biomarker research
  13. Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
  14. Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
  15. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
  16. Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
  17. Whole-cell optical biosensor for mercury – operational conditions in saline water
  18. Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
  19. Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
  20. Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
  21. Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
  22. Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
  23. Can voltammetry distinguish glycan isomers?
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