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Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex-DNA interactions

  • Ahmet Colak EMAIL logo , Ender Cekirge , Serdar Karaböcek , Aslıgül Küçükdumlu , Nagihan Ertunga , Melek Col and Rza Abbasoğlu
Published/Copyright: August 25, 2009
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Chemical Papers
From the journal Chemical Papers Volume 63 Issue 5

Abstract

Nucleolytic activities of some new oxime-type ligand complexes were investigated by neutral agarose gel electrophoresis. Analysis of the cleavage products in agarose gel indicated that all complexes used converted supercoiled pUC18 plasmid DNA to its nicked or linear form. It was found that nucleolytic activities of the complexes depend on the complex concentration, reaction time and the presence of a cooxidant (magnesium monoperoxyphthalate, MMPP) in the reaction mixture. However, the complexes cleaved pUC18 plasmid DNA at all investigated pH values. Nucleolytic activities of complexes were investigated for different complex concentrations (0.1–100 μmol L−1), pH values (6.0–10.0) and reaction times (0–60 min). Molecular modeling studies performed by the Hyperchem Software together with DNA-binding studies showed that planar sites of the complexes intercalated into double stranded DNA. It can be concluded that all oxime-type ligand complexes used can be evaluated as nuclease mimics.

Keywords: intercalation; oxime-type ligand; oxidative cleavage; nuclease mimic

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Published Online: 2009-8-25
Published in Print: 2009-10-1

© 2009 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-009-0058-y
Keywords for this article
intercalation; oxime-type ligand; oxidative cleavage; nuclease mimic

Articles in the same Issue

  1. Magnetic nano- and microparticles in biotechnology
  2. Application of gas chromatography-mass spectrometry in research of traditional Chinese medicine
  3. Copper determination using ICP-MS with hexapole collision cell
  4. Reactivation of a palladium catalyst during glucose oxidation by molecular oxygen
  5. Robust stabilization of a chemical reactor
  6. Influence of production progress on the heavy metal content in flax fibers
  7. In vitro antifungal and antibacterial properties of thiodiamine transition metal complexes
  8. Synthesis, characterization, and antimicrobial activity of new benzoylthiourea ligands
  9. Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex-DNA interactions
  10. Characterization of mechanochemically synthesized lead selenide
  11. Hydroxyapatite modified with silica used for sorption of copper(II)
  12. Corrosion resistance of zinc electrodeposited from acidic and alkaline electrolytes using pulse current
  13. Ternary composites of multi-wall carbon nanotubes, polyaniline, and noble-metal nanoparticles for potential applications in electrocatalysis
  14. Synthesis of 2-[3-(trifluoromethyl)phenyl]furo[3,2-c]pyridine derivatives
  15. Key side products due to reactivity of dimethylmaleoyl moiety as amine protective group
  16. Comparative DFT study on the α-glycosidic bond in reactive species of galactosyl diphosphates
  17. Gas chromatographic retention times prediction for components of petroleum condensate fraction
  18. Gas chromatography with surface ionization detection of nitro pesticides
  19. Clean fuel-oriented investigation of thiophene oxidation by hydrogen peroxide using polyoxometalate as catalyst
  20. Aqueous foam stabilized by polyoxyethylene dodecyl ether
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