Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
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Lucie Maixnerová
,
Alexandar Horvitz
Abstract
A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid. The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic-organic hybrid polymer ORMOCER® and deposited on a lens to form a sensitive layer of 210 μm in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 μs L mmol−1 and 4.50 μs L mmol−1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensor sensitivity decreased from 6.87 μs L mmol−1 to 0.70 μs L mmol−1 and its dynamic range increased from 0.10 mmol L−1 to 1.75 mmol L−1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reaction-transport processes inside the sensing layer was developed. The model revealed the qualitative relations between the sensor analytical features, the characteristics of the sensitive layer and concentrations of substrates. The results of the mathematical modelling may serve as guidelines in the design of optodes for specific applications.
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Artikel in diesem Heft
- Biosensors – Topical issue
- Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
- Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review
- Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells
- Application of nanomaterials in microbial-cell biosensor constructions
- Use of green fluorescent proteins for in vitro biosensing
- Biosensors based on molecular beacons
- DNA aptamer-based detection of prostate cancer
- Can glycoprofiling be helpful in detecting prostate cancer?
- Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors
- Electrochemical nanostructured biosensors: carbon nanotubes versus conductive and semi-conductive nanoparticles
- Surface plasmon resonance application in prostate cancer biomarker research
- Improvement of enzyme carbon paste-based biosensor using carbon nanotubes for determination of water-soluble analogue of vitamin E
- Enzymatic sensor of putrescine with optical oxygen transducer – mathematical model of responses of sensitive layer
- Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
- Interfacing of microbial cells with nanoparticles: Simple and cost-effective preparation of a highly sensitive microbial ethanol biosensor
- Whole-cell optical biosensor for mercury – operational conditions in saline water
- Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization
- Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor
- Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform
- Interactions of antifouling monolayers: Energy transfer from excited albumin molecule to phenol red dye
- Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode
- Can voltammetry distinguish glycan isomers?
