Startseite Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model
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Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model

  • Sandesh R. Wayal ORCID logo und Shailendra S. Gurav ORCID logo EMAIL logo
Veröffentlicht/Copyright: 11. März 2021
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 18 Heft 3

Abstract

Objectives

Ghee is widely considered as the Indian name for clarified butterfat and processing of ghee with therapeutic herbs i.e., ghrita is renowned for augmenting their medicinal properties. The wound is considered as a challenging clinical problem with early and late complications. To reduce the burden of wounds with the shortest period and minimum scaring, an attempt was made to prepare and evaluate the wound healing potential of ghee based polyherbal formulation.

Methods

Based on local ethnic tribal claims, Semecarpus anacardium L., Argemone mexicana L., Cocculus hirsutus L., and Woodfordia fruticosa K. were collected from Western Ghats of India. The polyherbal Bhallatakadi Ghrita (BG) formulation was prepared as per Ayurvedic procedure and assessed for its wound healing potential using incision and excision wound animal models.

Results

BG treated group showed a complete contraction of wounds (99.82 ± 0.10%) (p<0.001) with 15.17 ± 0.40 days re-epithelization time and breaking strength (531.50 ± 5.89) (p<0.05). The hydroxyproline content of BG was found to be significantly higher i.e., 4.23 ± 0.21 (p<0.05). Quantitative estimation of BG exhibited 54.7 ± 3.7 mg100 g−1 of polyphenols and 42.3 ± 5.4 mg.100 g−1 flavonoids in terms of gallic acid and quercetin respectively. HPLC analysis revealed the presence of gallic acid and quercetin whereas the presence of fatty acids was confirmed by GC-MS analysis.

Conclusions

It may conclude that the presence of quercetin, gallic acid, and fatty acids could have accelerated the healing rate of the ghrita formulation, as they have already been known for their potential wound healing properties.

Keywords: Ayurveda ; ghrita ; HPLC; wound breaking strength; wound infection
Corresponding author: Dr. Shailendra S. Gurav, Department of Pharmacognosy, Goa College of Pharmacy, 18th June Road, Panaji, Goa 403 001, India, E-mail:

Acknowledgment

The authors would like to acknowledge Chaitanya laboratory, Pune for histological study and Central Instrumentation Facility, SPPU, Pune for analytical study.

  1. Research funding: None declared.

  2. Author contributions: Both the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  3. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  4. Ethical approval: The research related to the animals use has been compiled with the relevant national regulations and institutional policies for the care and use of animals.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/jcim-2020-0179).

Received: 2020-05-24
Accepted: 2020-08-12
Published Online: 2021-03-11

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. ‘Ilāj bi’l-Tadbīr (regimenal therapy): a core mode of Unani treatment
  4. Understanding hormones in terms of humours (Akhlat) in Unani system of medicine
  5. Review Articles
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  11. Neera, a non-fermented traditional drink from coconut spadix restores the redox status in sodium fluoride intoxicated mice
  12. Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model
  13. Effects of co-administration of Unani pharmacopoeia formulations Qurs Tabasheer Sartani and Arq Hara Bhara with CAT-I antitubercular drugs in rats
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  16. A spatial-temporal study of complementary and alternative medicine (CAM) by type: exploring localization economies implications in urban areas in Ontario
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  19. Effectiveness of wet cupping therapy on relieving pain in patients with chronic migraine: an observational study
  20. Effect of aromatherapy massage by orange essential oil on post-cesarean anxiety: a randomized clinical trial
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  22. Comparison of instability resistance training, traditional resistance training and plyometric training on athletic performance parameters
  23. Individualized homeopathic medicines and Urtica urens mother tincture in treatment of hyperuricemia: an open, randomized, pragmatic, pilot trial
  24. Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers
  25. Dietary glycine improves urine storage symptoms in urology outpatients
  26. Hypoxia-induced oxidative stress in high altitude population: impact of coenzyme Q10 supplementation
  27. Short Communications
  28. Terminalia arjuna induced testicular assault through Leydig cell derangement: an in vitro approach
  29. Effect of acupuncture on cognitive task performance of college students: a pilot study
  30. Yoga for children in the new normal – experience sharing
  31. Effect of Arogya Raksha Panchatantra (five lifestyle principles) on hematological parameters and anthropometric measures among healthy volunteers: a pilot study
  32. Case Reports
  33. Yellow phosphorous ingestion cause liver dysfunction and internal bleeding: a case study
  34. Effect of yoga on plasma glucose, lipid profile, blood pressure and insulin requirement in a patient with type 1 diabetes mellitus
  35. Management of Vipadika with Mahisha Gritha Padanimajjana – a case report
https://doi.org/10.1515/jcim-2020-0179
Schlagwörter für diesen Artikel
Ayurveda; ghrita; HPLC; wound breaking strength; wound infection

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. ‘Ilāj bi’l-Tadbīr (regimenal therapy): a core mode of Unani treatment
  4. Understanding hormones in terms of humours (Akhlat) in Unani system of medicine
  5. Review Articles
  6. Understanding Humma-e-Wabai (epidemic fever) and Amraz-e-Wabai (epidemic disease) in the light of Unani medicine
  7. Emergence of antimicrobial resistance and magnitude of Unani medicine – scope and challenges
  8. Medicinal importance of Papra (Podophyllum hexandrum Royle) in Unani System of Medicine
  9. Research Articles
  10. Comparative effects of berberine and piperine on the neuroprotective potential of neostigmine
  11. Neera, a non-fermented traditional drink from coconut spadix restores the redox status in sodium fluoride intoxicated mice
  12. Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model
  13. Effects of co-administration of Unani pharmacopoeia formulations Qurs Tabasheer Sartani and Arq Hara Bhara with CAT-I antitubercular drugs in rats
  14. Neurotoxicity of organic solvents with emphasis on the role of iron
  15. Regular consumption of “Nkui”, a Cameroonian traditional dish, may protect against cardiovascular and bone disorders in an estrogen deficiency condition
  16. A spatial-temporal study of complementary and alternative medicine (CAM) by type: exploring localization economies implications in urban areas in Ontario
  17. Complementary and alternative interventions for stroke recovery – a narrative overview of the published evidence
  18. The feasibility and acceptability of hypnotherapy among overweight and obese individuals in a Malaysia’s public university
  19. Effectiveness of wet cupping therapy on relieving pain in patients with chronic migraine: an observational study
  20. Effect of aromatherapy massage by orange essential oil on post-cesarean anxiety: a randomized clinical trial
  21. Effect of Sudarshan Kriya Yoga (SKY) on daytime and situational sleep propensity in novice practitioners: a prospective cohort study
  22. Comparison of instability resistance training, traditional resistance training and plyometric training on athletic performance parameters
  23. Individualized homeopathic medicines and Urtica urens mother tincture in treatment of hyperuricemia: an open, randomized, pragmatic, pilot trial
  24. Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers
  25. Dietary glycine improves urine storage symptoms in urology outpatients
  26. Hypoxia-induced oxidative stress in high altitude population: impact of coenzyme Q10 supplementation
  27. Short Communications
  28. Terminalia arjuna induced testicular assault through Leydig cell derangement: an in vitro approach
  29. Effect of acupuncture on cognitive task performance of college students: a pilot study
  30. Yoga for children in the new normal – experience sharing
  31. Effect of Arogya Raksha Panchatantra (five lifestyle principles) on hematological parameters and anthropometric measures among healthy volunteers: a pilot study
  32. Case Reports
  33. Yellow phosphorous ingestion cause liver dysfunction and internal bleeding: a case study
  34. Effect of yoga on plasma glucose, lipid profile, blood pressure and insulin requirement in a patient with type 1 diabetes mellitus
  35. Management of Vipadika with Mahisha Gritha Padanimajjana – a case report
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