Comparative effects of berberine and piperine on the neuroprotective potential of neostigmine
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Stephen Adeniyi Adefegha
Abstract
Objectives
This study examined effect of berberine and piperine on neuroprotective potential of neostigmine in the management of neurological disorders.
Methods
Berberine and neostigmine were weighed (30 g), dissolved in distilled water (30 mL) separately, while, 30 mg piperine was dissolved in ethanol (0.45 mL), made up to 30 mL with distilled water. Antioxidant activities in 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonate) radical (ABTS), Fe-chelation, ferric reducing properties (FRAP), nitric oxide (NO) and hydroxyl (OH) radical scavenging abilities and Fe2+, cisplatin and sodium nitroprusside (SNP) induced lipid peroxidation (LPO), and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoamine oxidase (MAO) activities were assessed in vitro.
Results
The result revealed that tested compounds inhibited enzymes activities dose-dependently. However, berberine (IC50=0.17 mg/mL) had slight higher AChE inhibitory effect than piperine and neostigmine (p<0.05). Also, berberine had the highest BChE inhibitory effect (IC50=0.16 mg/mL) while piperine exhibited the highest MAO inhibitory effect (IC50=0.21 mg/mL). Berberine, piperine and neostigmine exhibited high antioxidant properties and inhibited Fe2+, cisplatin and SNP induced LPO.
Conclusions
Both alkaloids demonstrated antiradical scavenging ability comparable to neostigmine action against Alzheimer’s disease (AD). The modulatory and antioxidant berberine and piperine properties on these enzymes (AChE, BChE and MAO) could be possible underlying mechanisms in employing these compounds as a complementary therapy in neurodegenerative diseases (NDDs) management.
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Research funding: None declared.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Competing interests: No funding organizations played a role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Ethical statement: Primary data for human nor for animals were not collected for this research work.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- ‘Ilāj bi’l-Tadbīr (regimenal therapy): a core mode of Unani treatment
- Understanding hormones in terms of humours (Akhlat) in Unani system of medicine
- Review Articles
- Understanding Humma-e-Wabai (epidemic fever) and Amraz-e-Wabai (epidemic disease) in the light of Unani medicine
- Emergence of antimicrobial resistance and magnitude of Unani medicine – scope and challenges
- Medicinal importance of Papra (Podophyllum hexandrum Royle) in Unani System of Medicine
- Research Articles
- Comparative effects of berberine and piperine on the neuroprotective potential of neostigmine
- Neera, a non-fermented traditional drink from coconut spadix restores the redox status in sodium fluoride intoxicated mice
- Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model
- Effects of co-administration of Unani pharmacopoeia formulations Qurs Tabasheer Sartani and Arq Hara Bhara with CAT-I antitubercular drugs in rats
- Neurotoxicity of organic solvents with emphasis on the role of iron
- Regular consumption of “Nkui”, a Cameroonian traditional dish, may protect against cardiovascular and bone disorders in an estrogen deficiency condition
- A spatial-temporal study of complementary and alternative medicine (CAM) by type: exploring localization economies implications in urban areas in Ontario
- Complementary and alternative interventions for stroke recovery – a narrative overview of the published evidence
- The feasibility and acceptability of hypnotherapy among overweight and obese individuals in a Malaysia’s public university
- Effectiveness of wet cupping therapy on relieving pain in patients with chronic migraine: an observational study
- Effect of aromatherapy massage by orange essential oil on post-cesarean anxiety: a randomized clinical trial
- Effect of Sudarshan Kriya Yoga (SKY) on daytime and situational sleep propensity in novice practitioners: a prospective cohort study
- Comparison of instability resistance training, traditional resistance training and plyometric training on athletic performance parameters
- Individualized homeopathic medicines and Urtica urens mother tincture in treatment of hyperuricemia: an open, randomized, pragmatic, pilot trial
- Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers
- Dietary glycine improves urine storage symptoms in urology outpatients
- Hypoxia-induced oxidative stress in high altitude population: impact of coenzyme Q10 supplementation
- Short Communications
- Terminalia arjuna induced testicular assault through Leydig cell derangement: an in vitro approach
- Effect of acupuncture on cognitive task performance of college students: a pilot study
- Yoga for children in the new normal – experience sharing
- Effect of Arogya Raksha Panchatantra (five lifestyle principles) on hematological parameters and anthropometric measures among healthy volunteers: a pilot study
- Case Reports
- Yellow phosphorous ingestion cause liver dysfunction and internal bleeding: a case study
- Effect of yoga on plasma glucose, lipid profile, blood pressure and insulin requirement in a patient with type 1 diabetes mellitus
- Management of Vipadika with Mahisha Gritha Padanimajjana – a case report
Articles in the same Issue
- Frontmatter
- Reviews
- ‘Ilāj bi’l-Tadbīr (regimenal therapy): a core mode of Unani treatment
- Understanding hormones in terms of humours (Akhlat) in Unani system of medicine
- Review Articles
- Understanding Humma-e-Wabai (epidemic fever) and Amraz-e-Wabai (epidemic disease) in the light of Unani medicine
- Emergence of antimicrobial resistance and magnitude of Unani medicine – scope and challenges
- Medicinal importance of Papra (Podophyllum hexandrum Royle) in Unani System of Medicine
- Research Articles
- Comparative effects of berberine and piperine on the neuroprotective potential of neostigmine
- Neera, a non-fermented traditional drink from coconut spadix restores the redox status in sodium fluoride intoxicated mice
- Evaluation of wound healing potential of Bhallatakadi Ghrita – cow ghee based polyherbal formulation: in-vivo excision and incision wound model
- Effects of co-administration of Unani pharmacopoeia formulations Qurs Tabasheer Sartani and Arq Hara Bhara with CAT-I antitubercular drugs in rats
- Neurotoxicity of organic solvents with emphasis on the role of iron
- Regular consumption of “Nkui”, a Cameroonian traditional dish, may protect against cardiovascular and bone disorders in an estrogen deficiency condition
- A spatial-temporal study of complementary and alternative medicine (CAM) by type: exploring localization economies implications in urban areas in Ontario
- Complementary and alternative interventions for stroke recovery – a narrative overview of the published evidence
- The feasibility and acceptability of hypnotherapy among overweight and obese individuals in a Malaysia’s public university
- Effectiveness of wet cupping therapy on relieving pain in patients with chronic migraine: an observational study
- Effect of aromatherapy massage by orange essential oil on post-cesarean anxiety: a randomized clinical trial
- Effect of Sudarshan Kriya Yoga (SKY) on daytime and situational sleep propensity in novice practitioners: a prospective cohort study
- Comparison of instability resistance training, traditional resistance training and plyometric training on athletic performance parameters
- Individualized homeopathic medicines and Urtica urens mother tincture in treatment of hyperuricemia: an open, randomized, pragmatic, pilot trial
- Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers
- Dietary glycine improves urine storage symptoms in urology outpatients
- Hypoxia-induced oxidative stress in high altitude population: impact of coenzyme Q10 supplementation
- Short Communications
- Terminalia arjuna induced testicular assault through Leydig cell derangement: an in vitro approach
- Effect of acupuncture on cognitive task performance of college students: a pilot study
- Yoga for children in the new normal – experience sharing
- Effect of Arogya Raksha Panchatantra (five lifestyle principles) on hematological parameters and anthropometric measures among healthy volunteers: a pilot study
- Case Reports
- Yellow phosphorous ingestion cause liver dysfunction and internal bleeding: a case study
- Effect of yoga on plasma glucose, lipid profile, blood pressure and insulin requirement in a patient with type 1 diabetes mellitus
- Management of Vipadika with Mahisha Gritha Padanimajjana – a case report
