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Molecular characterization and phylogeny of some mazocraeidean monogeneans from carangid fish

  • Neeraja Tambireddy , Tripathi Gayatri , Pathakota Gireesh-Babu and Annam Pavan-Kumar EMAIL logo
Published/Copyright: March 30, 2016
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Acta Parasitologica
From the journal Acta Parasitologica Volume 61 Issue 2

Abstract

Polyopisthocotylean monogenean parasites of fishes are highly host specific and have been used as an appropriate model to study the host-parasite co-evolution. In the present study, eight monogeneans of the order Mazocraeidea were characterized by nuclear 28S rDNA sequences and their phylogenetic relationship with other polyopisthocotylean species was investigated. Neighbour-joining, maximum parsimony, maximum likelihood and Bayesian Inference methods were used for phylogenetic reconstruction. The topology sustained by high bootstrap was: (((Hexabothriidae (Mazocraeidae (Discocotylidae (Diplozoidae (Diclidophoridae (Plectanocotylidae (Heteromicrocotylidae (Microcotylidae (Heteraxinidae), (Thoracocotylidae, Gotocotylidae (Gastrocoylidae (Allodiscocotylidae: Protomicrocotylidae))). In addition, we have also developed DNA barcodes (COI sequences) for six species and the barcodes clearly discriminated all the species. The polytomy within Protomicrocotylidae family is resolved in this study for the first time and it appears that within this family, Bilaterocotyloides species are basal compared to Neomicrocotyle and Lethacotyle species while the latter is the more derived.

Keywords: Monogeneans; Polyopisthocotylea; molecular phylogeny; 28S rDNA; DNA barcoding

Acknowledgements

The authors are thankful to Dr. W. S. Lakra, the Director and Vice Chancellor of the Central Institute of Fisheries Education (CIFE), Mumbai, India, for providing all the facilities required to carry out the present study.

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Received: 2015-6-9
Revised: 2015-10-23
Accepted: 2015-12-11
Published Online: 2016-3-30
Published in Print: 2016-6-1

© W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2016-0047
Keywords for this article
Monogeneans; Polyopisthocotylea; molecular phylogeny; 28S rDNA; DNA barcoding

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