Genetic diversity of Diplomonadida in fish of the genus Coregonus from Southeastern Siberia

Natalia Denikina; Ivan Nebesnykh; Olga Maikova; Elena Dzyuba; Natalia Belkova

doi:10.1515/ap-2016-0040

Article

Genetic diversity of Diplomonadida in fish of the genus Coregonus from Southeastern Siberia

Natalia Denikina , Ivan Nebesnykh , Olga Maikova , Elena Dzyuba and Natalia Belkova

Published/Copyright: March 30, 2016

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From the journal Acta Parasitologica Volume 61 Issue 2

Abstract

Diplomonadida are primitive flagellate protozoa, among which both commensals and pathogens have been recorded. To date, members of the genera Hexamita and Spironucleus have been reported in the digestive system of fish in the Baikal region. We determined the genetic diversity of Diplomonadida in fish of the genus Coregonus from south-eastern Siberia using molecular-genetic methods. Fish for analysis were caught in Lake Baikal and in the Barguzin, Nepa, Chechuy, and Kirenga rivers from 2010 to 2013. Gall bladders, hindguts and foreguts of 120 specimens of Coregonus migratorius representing three morpho-ecological groups, 25 specimens of Coregonus lavaretus baicalensis, 25 specimens of Coregonus tugun and 30 specimens of Coregonus lavaretus pidschian were analysed via amplification with primers specifically designed for eukaryotes. Amplicons positive for Diplomonadida were sequenced. A phylogenetic analysis revealed that diplomonad flagellates of whitefish from Southeastern Siberia belong to Spironucleus barkhanus. Positive Diplomonadida DNA samples were analysed with primers designed in the present study for the amplification of small subunits of ribosomal DNA fragments of S. barkhanus (about 1,430 bp) and sequenced. Phylogenetic analysis revealed inside the clade of S. barkhanus besides the cosmopolitan genotype from European salmon that was detected earlier in Baikalian grayling, a new genotype unique to the fish of the genus Coregonus from Lake Baikal.

Key words: Coregonus; Spironucleus; specific primers; SSU rRNA gene; phylogenetic analysis; Southeastern Siberia

Acknowledgements

The authors thank Lubov Sukhanova, Yuliya Sapozhnikova and Sergey Kirilchik for help with sample collection. This research was performed as part of the VI.50.1.4 project ‘Molecular Ecology and Evolution’ (#0345–2014–0002).

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Received: 2015-7-30

Revised: 2015-10-20

Accepted: 2015-12-4

Published Online: 2016-3-30

Published in Print: 2016-6-1

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https://doi.org/10.1515/ap-2016-0040

Keywords for this article

Coregonus; Spironucleus; specific primers; SSU rRNA gene; phylogenetic analysis; Southeastern Siberia