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Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil

  • Laura Fuhrich Fabres , Sayonara Peixoto Rosa dos Santos , Lisianne Brittes Benitez and Marilise Brittes Rott EMAIL logo
Published/Copyright: March 30, 2016
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Acta Parasitologica
From the journal Acta Parasitologica Volume 61 Issue 2

Abstract

Free-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Species of Acanthamoeba can cause keratitis and brain infections. In this study, 72 water samples were taken from both hot tubs and thermal swimming pools in the city of Porto Alegre, RS, Brazil, to determine the presence of Acanthamoeba in the water as well as perform the phenotypic and genotypic characterization of the isolates. The identification of the isolates was based on the cysts morphology and PCR amplification using genus-specific oligonucleotides. When the isolates were submitted to PCR reaction only 8 were confirmed as belonging to the genus Acanthamoeba. The sequences analysis when compared to the sequences in the GenBank, showed genotype distribution in group T3 (12,5%), T5 (12,5%), T4 (25%) and T15 (50%). The results of this study confirmed the presence of potentially pathogenic isolates of free living amoebae in hot swimming pool and spas which can present risks to human health.

Key words: Acanthamoeba; thermal swimming-pool; hot tubs; genotypes; environmental isolates

Acknowledgements

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support, the Microbiology, Immunology and Parasitology Department of the Universidade Federal do Rio Grande do Sul, Brazil

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Received: 2015-4-4
Revised: 2015-9-29
Accepted: 2015-11-25
Published Online: 2016-3-30
Published in Print: 2016-6-1

© W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2016-0031
Keywords for this article
Acanthamoeba; thermal swimming-pool; hot tubs; genotypes; environmental isolates

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