Morphological, molecular and developmental characterization of the thelastomatid nematode Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasite of Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) in Japan

Sota Ozawa; Jans Morffe; Cláudia S.L. Vicente; Kenji Ikeda; Ryoji Shinya; Koichi Hasegawa

doi:10.1515/ap-2016-0034

Article

Morphological, molecular and developmental characterization of the thelastomatid nematode Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasite of Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) in Japan

Sota Ozawa , Jans Morffe , Cláudia S.L. Vicente , Kenji Ikeda , Ryoji Shinya and Koichi Hasegawa

Published/Copyright: March 30, 2016

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From the journal Acta Parasitologica Volume 61 Issue 2

Abstract

The American cockroach Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) has been spreading worldwide by commerce and has successfully adjusted to living with humans. There are many reports of thelastomatid parasitic nematode isolated from P. americana in many countries including USA, Canada, India, Argentina, Bulgaria, but not in Japan. We have investigated the parasitic nematodes in P. americana lab strain and field-captured individuals in Japan and found that Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasitizes with high infection rates. We described morphological, molecular, and developmental characters of the parasitic nematode because such information was missing despite it has been discovered more than one hundred years ago. We described morphometrics with DIC microscopy and fine structure of male and female adult with SEM observation. We also reveal the embryonic and postembryonic development of this nematode. This is the first report of a thelastomatid nematode isolated from American cockroach in Japan, and the data showed here is also very useful and fundamental for further analysis of the cockroach and parasite relations.

Key words: Thelastoma bulhoesi; American cockroach; taxonomy; systematics; embryogenesis; post-embryogenesis

Sota Ozawa and Jans Morffe contributed equally to this work and should be consider as first co-authors

Acknowledgements

The American cockroach was provided by Nissan Chemical Co. Ltd., Saitama, Japan. We thank Dr. Rina Sriwati for technical assistance and valuable advice. This work was supported by the research fund from Institute of the Biological Function, Chubu University (to KH), and JSPS Research Fellowship for Young Scientists (to SO).

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Received: 2015-6-18

Revised: 2015-10-23

Accepted: 2015-11-30

Published Online: 2016-3-30

Published in Print: 2016-6-1

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Keywords for this article

Thelastoma bulhoesi; American cockroach; taxonomy; systematics; embryogenesis; post-embryogenesis