Synthesis, DNA binding, and antimicrobial studies of novel metal complexes containing a pyrazolone derivative Schiff base
-
Natarajan Raman
,
Ramaraj Jeyamurugan
Abstract
A novel series of Co(II), Ni(II), Cu(II), Zn(II), and VO(IV) complexes has been synthesized from the Schiff base derived from 4-[(3,4-dimethoxybenzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one and 1,2-diaminobenzene. Structural features were determined by analytical and spectral techniques. Binding of synthesized complexes with calf thymus DNA (CT DNA) was studied by spectroscopic methods and viscosity measurements. Experimental results indicate that the complexes are able to form adducts with DNA and to distort the double helix by changing the base stacking. Lower DNA affinity of the VO(IV) complex is caused by the change of coordination geometry by the vanadyl ion resulting in a somewhat unfavorable configuration for the DNA binding. Oxidative DNA cleavage activities of the complexes were studied with supercoiled (SC) pUC19 DNA using gel electrophoresis; the mechanism studies revealed that the hydroxyl radical is likely to be the reactive species responsible for the cleavage of pUC19 DNA by the synthesized complexes. The in vitro antimicrobial screening effects of the investigated compounds were monitored by the disc diffusion method. The synthesized Schiff base complexes exhibit higher antimicrobial activity than the respective free Schiff base.
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© 2009 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- A proposal of reference values for relative uncertainty increase in spectrophotometric analysis of pharmaceutical formulations
- Spectrophotometric quantification of fluoxetine hydrochloride: Application to quality control and quality assurance processes
- A simple turbidimetric flow injection system for saccharin determination in sweetener products
- Determination of metoprolol tartrate by capillary isotachophoresis
- Model predictive control of a CSTR: A hybrid modeling approach
- Application of extended NRTL equation for ternary liquid-liquid and vapor-liquid-liquid equilibria description
- Synthesis, DNA binding, and antimicrobial studies of novel metal complexes containing a pyrazolone derivative Schiff base
- Synthesis, spectral and electrochemical study of coordination molecules Cu4OX6L4: 4-cyanopyridine Cu4OBrnCl(6−n)(4-CNpy)4 complexes
- Synthesis, spectral and electrochemical study of coordination molecules Cu4OX6L4: 3-cyanopyridine Cu4OBrnCl(6−n)(3-CNpy)4 complexes
- Deposition and release of chlorhexidine from non-ionic and anionic polymer matrices
- Synthesis of new antimicrobial 4-aminosubstituted 3-nitrocoumarins
- Spectroscopic characterization of halogen- and cyano-substituted pyridinevinylenes synthesized without catalyst or solvent
- Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. (Geraniaceae)
- Photo-Fenton and photo-Fenton-like processes for the degradation of methyl orange in aqueous medium: Influence of oxidation states of iron
- Voltammetry of resazurin at a mercury electrode
- Effect of dielectric medium on angiotensin converting enzyme inhibitors binding to Zn2+
- HPLC analysis of a syrup containing nimesulide and its hydrolytic degradation product
