Determination of 10B in lymphoma human cells after boron carrier treatment: comparison of 10BPA and immuno-nanoparticles
-
Marco Persico
,
Patrizia Chiari
Abstract
Current cancer treatments lead to insufficient distribution of therapeutic agents in tumor cells due to their lack of selectivity often causing adverse effects in the normal cell uptake of the drug. The challenge is to design therapeutic drugs able to maximize the treatment of malignant cancers while minimizing their adverse effects. In this study, 10B incorporation in the B chronic lymphocytic leukemia cell line MEC-1 was investigated. The cells were treated with l-p-10borophenylalanine (10BPA) and 10boron immuno-nanoparticles (BNPs) loaded with 10BPA with or without the anti-CD20 monoclonal antibody for different contact times. The uptake of 10B by the cells was determined by the inductively coupled plasma mass spectrometry (ICP-MS) after acid mineralization. To obtain accurate and consistent data, the analytical procedure was optimized using factorial experimental design. It was observed that BNP loaded with 10BPA and anti-CD20 represents the best carrier system for 10B in B cells for long time (>10 h) whereas 10BPA seems to be the top drug for short time (< 4 h) procedures.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
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Articles in the same Issue
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- Synthesis of ethyl-6-aminohexanoate from caprolactam and ethanol in near-critical water
- Vanadium dodecylamino phosphate: A novel efficient catalyst for synthesis of polyhydroquinolines
- Modelling and experimental validation of enantioseparation of racemic phenylalanine via a hollow fibre-supported liquid membrane
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- Mercury associated with size-fractionated urban particulate matter: three years of sampling in Prague, Czech Republic
- Chemical composition and antioxidant activity of sulphated polysaccharides extracted from Fucus vesiculosus using different hydrothermal processes
- A new organically templated magnesium sulfate: structure, spectroscopic analysis, and thermal behaviour
- Synthesis, characterization and photoluminescence properties of Ce3+-doped ZnO-nanophosphors
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