Phytochemical characterisation and toxicity effect of Tithonia diversifolia (Hemls.) A. Gray leaf extract on fall army worm Spodoptera frugiperda (JE Smith) larvae
-
Cecilia Oluwamodupe
Abstract
Objectives
To mitigate the losses due to fall armyworm (FAW) infestation in maize, chemical pesticides had been the first choice and widely used as an emergency response. However, it comes with attendant health effect. This necessitates the development of plant based effective and safer pesticides. This research investigate response of fall armyworm larvae when they are exposed to crude and partially purified Tithonia diversifolia leaf extract.
Methods
Chemical constituent of the extract was identified using NIST08.LIB library spectra provided by the software on a GC-MS system and FTIR analysis was done using KBr pellet technique with a resolution and scanning speed of 4 cm−1 and 2 mm/s. Dose dependent toxicity assay of T. diversifolia extracts on FAW at different growth stages under controlled environment in laboratory, followed by its effect under phytotron were examined against control and azadirachtin from neem.
Results
The GC-MS of the butanol eluent revealed 20 compounds out of which the major ones being beta-d-glucopyranoside, methyl (15.225 %) palmitic acid, TMS derivative (10.98 %) and hexadecanoic acid, 2-[(trimethylsily)oxy]-, methyl ester (8.75 %). The FT-IR spectroscopic analysis of the butanol eluent of T. diversifolia leaf extract revealed the presence of alcohols, phenols, aldehydes, ketones, alkanes and primary amines. The butanol eluent and crude extract caused 96 % mortality at neonate and first instar FAW larvae.
Conclusions
The toxic and repellant effects revealed by diet bioassay and phytotron experiment respectively suggest that butanol eluent of T. diversifolia leaf extract could be a good and effective target for biopesticide production against FAW.
Funding source: Department of Biotechnology India -The world academy of science Trieste Italy under DBT-TWAS Postdoctoral fellowship award
Funding source: Tertiary Education Trust Fund Nigeria Under TETFUND Institutional based research 2020 Intervention
Acknowledgments
We acknowledge Directors of ICAR-IARI and ICAR-IIMR for the facilities. We thank Dr. Aditi Kundu for providing azadirachtin 29 %.
-
Research ethics: Not applicable.
-
Informed consent: Not applicable.
-
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
-
Competing interests: Authors state no conflict of interest.
-
Research funding: The project was funded by: Tertiary Education Trust Fund Nigeria under TETFUND Institutional based research 2020 Intervention, and Department of Biotechnology India – The World Academy of Science Trieste Italy under DBT-TWAS Postdoctoral fellowship award.
-
Data availability: Not applicable.
References
1. Johnson, SJ. Migration and the life history strategy of the fall armyworm, Spodoptera frugiperda in the western hemisphere. Int J Trop Insect Sci 1987;8:543–9. https://doi.org/10.1017/s1742758400022591.Suche in Google Scholar
2. Odeyemi, O, Ugwu, J. Fall armyworm, Spodoptera frugiperda outbreak in Nigeria: impacts and management on maize fields – a review. Ethiop J Environ Stud Manag 2021;1:14–9.Suche in Google Scholar
3. Goergen, G, Kumar, PL, Sankung, SB, Togola, A, Tamò, M. First report of outbreaks of the fall armyworm Spodoptera frugiperda (JE Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa. PLoS One 2016;11:e0165632. https://doi.org/10.1371/journal.pone.0165632.Suche in Google Scholar PubMed PubMed Central
4. Day, R, Abrahams, P, Bateman, M, Beale, T, Clottey, V, Cock, M, et al.. Fall armyworm: impacts and implications for Africa. Outlooks Pest Manag 2007;28:196–201. https://doi.org/10.1564/v28_oct_02.Suche in Google Scholar
5. Kalleshwaraswamy, CM, Asokan, R, Swamy, HM, Maruthi, MS, Pavithra, HB, Hegbe, K, et al.. First report of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), an alien invasive pest on maize in India. Indian J Entomol 2018;80:540–3.10.5958/0974-8172.2018.00238.9Suche in Google Scholar
6. Pest reports. International plant protection convention; 2022. Available from: https://www.ippc.int/.Suche in Google Scholar
7. Prasanna, BM, Huesing, JE, Eddy, R, Peschke, VM. Fall armyworm in Africa: a guide for integrated pest management; 2018.Suche in Google Scholar
8. Ogunfunmilayo, AO, Kazeem, SA, Idoko, JE, Adebayo, RA, Fayemi, EY, Adedibu, OB, et al.. Occurrence of natural enemies of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) in Nigeria. PLoS One 2021;16:e0254328. https://doi.org/10.1371/journal.pone.0254328.Suche in Google Scholar PubMed PubMed Central
9. Pratheeba, T, Vivekanandhan, P, Nur Faeza, AK, Natarajan, D. Chemical constituents and larvicidal efficacy of Naringi crenulata (Rutaceae) plant extracts and bioassay guided fractions against Culex quinquefasciatus mosquito (Diptera: Culicidae). Biocatal Agric Biotechnol 2019;19:101137. https://doi.org/10.1016/j.bcab.2019.101137.Suche in Google Scholar
10. Vivekanandhan, P, Nanthini, AUR, Valli, G, Shivakumar, MS. Comparative efficacy of Eucalyptus globulus (Labill) hydrodistilled essential oil and temephos as mosquito larvicide. Nat Prod Res 2020;34:2626–29. https://doi.org/10.1080/14786419.2018.1547290.Suche in Google Scholar PubMed
11. Vivekanandhan, P, Senthil-Nathan, S, Shivakumar, MS. Larvicidal, pupicidal and adult smoke toxic effects of Acanthospermum hispidum (DC) leaf crude extracts against mosquito vectors. Physiol Mol Plant Pathol 2018;101:156–62. https://doi.org/10.1016/j.pmpp.2017.05.005.Suche in Google Scholar
12. Ascher, KS. Nonconventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Arch Insect Biochem Physiol 1993;22:433–49. https://doi.org/10.1002/arch.940220311.Suche in Google Scholar
13. Ejelonu, OC, Elekofehinti, OO, Adanlawo, IG. Tithonia diversifolia saponin-blood lipid interaction and its influence on immune system of normal wistar rats. Biomed Pharmacother 2017;87:589–95. https://doi.org/10.1016/j.biopha.2017.01.017.Suche in Google Scholar PubMed
14. Phambala, K, Tembo, Y, Kasambala, T, Kabambe, VH, Stevenson, PC, Belmain, SR. Bioactivity of common pesticidal plants on fall armyworm larvae (Spodoptera frugiperda). Plants 2020;9:112–6. https://doi.org/10.3390/plants9010112.Suche in Google Scholar PubMed PubMed Central
15. Gitahi, SM, Piero, MN, Mburu, DN, Machocho, AK. Repellent effects of selected organic leaf extracts of Tithonia diversifolia (Hemsl.) A. Gray and Vernonia lasiopus (O. Hoffman) against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). Sci World J 2021;15:26–33.10.1155/2021/2718629Suche in Google Scholar PubMed PubMed Central
16. Chidi, N, Constance, N, Nosiri, CI, Nwadike, C. Gas chromatography mass spectrometry/Fourier transform infrared (GC-MS/FTIR) spectral analyses of Tithonia diversifolia (Hemsl.) A. Gray leaves. J Med Plants Res 2017;11:345–50. https://doi.org/10.5897/jmpr2017.6391.Suche in Google Scholar
17. Chandrasekaran, M, Senthilkumar, A, Venkatesalu, V. Antibacterial and antifungal efficacy of fatty acid methyl esters from the leaves of Sesuvium portulacastrum L. Eur Rev Med Pharmacol Sci 2011;15:775–80.Suche in Google Scholar
18. Ogundare, AO. Antimicrobial effect of Tithonia diversifolia and Jatropha gossypifolia leaf extracts. Trends Appl Sci Res 2007;2:145–50. https://doi.org/10.3923/tasr.2007.145.150.Suche in Google Scholar
19. Oyewole, IO, Adeoye, GO, Anyasor, GN, Obansa, JA. Anti-malarial and repellent activities of Tithonia diversifolia (Hemsl.) leaf extracts. J Med Plants Res 2008;2:171–5.Suche in Google Scholar
20. Omotuyi, OI, Ukwenya, VO, Nash, O, Gbadamosi, AE, Ejelonu, OC, Inyang, KO. Synergistic erythropoietic mechanisms of Chromolaena odorata and Tithonia diversifolia in the bone marrow of Wistar rats. Comp Clin Pathol 2021;30:191–8. https://doi.org/10.1007/s00580-021-03216-1.Suche in Google Scholar
21. Yang, YC, Lee, EH, Lee, HS, Lee, DK, Ahn, YJ. Repellency of aromatic medicinal plant extracts and a steam distillate to Aedes aegypti. J Am Mosq Control Assoc 2004;20:146–9.Suche in Google Scholar
22. Pavela, R, Benelli, G. Ethnobotanical knowledge on botanical repellents employed in the African region against mosquito vectors – a review. Exp Parasitol 2016;167:103–8. https://doi.org/10.1016/j.exppara.2016.05.010.Suche in Google Scholar PubMed
© 2024 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- Boldine: a narrative review of the bioactive compound with versatile biological and pharmacological potential
- Potential anti-cancer activity of Moringa oleifera derived bio-active compounds targeting hypoxia-inducible factor-1 alpha in breast cancer
- Research Articles
- Phytochemical characterisation and toxicity effect of Tithonia diversifolia (Hemls.) A. Gray leaf extract on fall army worm Spodoptera frugiperda (JE Smith) larvae
- Management of wounds in diabetes by administering allicin and quercetin in emulsion form as wound medicine in diabetic rat models
- Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of Echinops kebericho Mesfin (Asteraceae) in mice model
- A spectrometric analysis of variedly purified cinnabar in a siddha drug – linga chendhooram
- Palm oil amends serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats
- Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats
- Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy
- Pharmacognostical characterization, GC-MS profiling, and elemental analysis of Curcuma caesia Roxb. rhizomes for public health
- Antibacterial activity of Macrosciadium alatum (M.Bieb.) plant extract
- Effect of horticultural therapy on static, dynamic balance and gait speed among institutionalized older adults with cognitive impairment
- Anti-hyperlipidemic effects of 500 mg spilanthol (SA3X) supplementation in people with dyslipidemia – a randomized, parallel-group placebo-controlled trial
- Potential biomarkers of ASD a target for future treatments: oxidative stress, chemokines, apoptotic, and methylation capacity
- Therapeutic ayurvedic interventions for the management of rheumatoid arthritis complicated by adhesive capsulitis – a case report
Artikel in diesem Heft
- Frontmatter
- Reviews
- Boldine: a narrative review of the bioactive compound with versatile biological and pharmacological potential
- Potential anti-cancer activity of Moringa oleifera derived bio-active compounds targeting hypoxia-inducible factor-1 alpha in breast cancer
- Research Articles
- Phytochemical characterisation and toxicity effect of Tithonia diversifolia (Hemls.) A. Gray leaf extract on fall army worm Spodoptera frugiperda (JE Smith) larvae
- Management of wounds in diabetes by administering allicin and quercetin in emulsion form as wound medicine in diabetic rat models
- Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of Echinops kebericho Mesfin (Asteraceae) in mice model
- A spectrometric analysis of variedly purified cinnabar in a siddha drug – linga chendhooram
- Palm oil amends serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats
- Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats
- Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy
- Pharmacognostical characterization, GC-MS profiling, and elemental analysis of Curcuma caesia Roxb. rhizomes for public health
- Antibacterial activity of Macrosciadium alatum (M.Bieb.) plant extract
- Effect of horticultural therapy on static, dynamic balance and gait speed among institutionalized older adults with cognitive impairment
- Anti-hyperlipidemic effects of 500 mg spilanthol (SA3X) supplementation in people with dyslipidemia – a randomized, parallel-group placebo-controlled trial
- Potential biomarkers of ASD a target for future treatments: oxidative stress, chemokines, apoptotic, and methylation capacity
- Therapeutic ayurvedic interventions for the management of rheumatoid arthritis complicated by adhesive capsulitis – a case report
