Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of Echinops kebericho Mesfin (Asteraceae) in mice model

Tesfaye Yimer Tadesse; Samuel Berihun Dagnew; Tesfagegn Gobezie Yiblet; Getu Tesfaw Addis; Zemene Demelash Kiflie

doi:10.1515/jcim-2024-0133

Article

Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of Echinops kebericho Mesfin (Asteraceae) in mice model

Tesfaye Yimer Tadesse , Samuel Berihun Dagnew , Tesfagegn Gobezie Yiblet , Getu Tesfaw Addis and Zemene Demelash Kiflie

Published/Copyright: June 19, 2024

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From the journal Journal of Complementary and Integrative Medicine Volume 21 Issue 3

Abstract

Objective

The present study was aimed at investigating the antinociceptive and anti-inflammatory activities of the solvent fractions of the roots of Echinops kebericho Mesfin in rodent models of pain and inflammation.

Methods

Successive maceration was used as a method of extraction using solvents of increasing polarity: methanol and water. Ethyl acetate, chloroform and distilled water were used as solvents of the fraction process. Swiss albino mice models were used in acetic acid induced writhing, hot plate, carrageenan induced paw edema and cotton pellet granuloma to assess the analgesic and anti-inflammatory activities. The test groups received different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) of the three fractions (chloroform, ethyl acetate and aqueous). The positive control groups received ASA (150 mg/kg) for the writing test, morphine (10 mg/kg) for the hot plate method, diclofenac Na for carrageenan-induced paw edema, and dexamethasone (10 mg/kg) for granuloma, while the negative control group received distilled water.

Results

EA fraction at all test doses employed (100 mg/kg, 200 mg/kg, and 400 mg/kg) showed statistically significant (p<0.05, p<0.01, p<0.001 respectively) analgesic and anti-inflammatory activities in a dose-dependent manner. The AQ fraction on the other hand produced statistically significant (p<0.05, p<0.012) analgesic and anti-inflammatory activities at the doses of 200 mg/kg and 400 mg/kg, while the CH fraction exhibited statistically significant (p<0.05) analgesic and anti-inflammatory activity at the dose of 400 mg/kg.

Conclusions

In general, the data obtained from the present study elucidated that the solvent fractions of the study plant possessed significant analgesic and anti-inflammatory activities and were recommended for further investigations.

Keywords: antinociceptive activity; anti-inflammatory activity; Echinops kebericho M.; hot plate; cotton pellet granuloma; carrageenan-induced paw edema

Corresponding author: Tesfaye Yimer Tadesse, Department of Pharmacy, Health Science College, Debre Tabor University, Debre Tabor, Ethiopia, E-mail: yimertesfaye00@gmail.com

Acknowledgments

The authors would like to acknowledge Debre Tabor University and the Department of Pharmacology, School of Pharmacy, College of Medicine and Health Science, University of Gondar.

Research ethics: Ethical clearance was obtained from the Research and Ethics Committee, College of Health Science, Debre Tabor University to conduct the study in an animal model with the reference number CHS 07-105-21.
Informed consent: Not applicable.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Research funding: None declared.
Data availability: All data that are analyzed are available on the hand of the corresponding author upon reasonable request.

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Received: 2024-04-29

Accepted: 2024-05-27

Published Online: 2024-06-19

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https://doi.org/10.1515/jcim-2024-0133

Keywords for this article

antinociceptive activity; anti-inflammatory activity; Echinops kebericho M.; hot plate; cotton pellet granuloma; carrageenan-induced paw edema