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Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats

  • Agatha Cristie Menezes , Michele Lima Brito EMAIL logo , Patricia Pereira Almeida , Beatriz Oliveira Da Cruz ORCID logo , Nathalia da Silva Costa ORCID logo , Aline D’Avila Pereira , Cecilia Castañon , Viviane Alexandre Nunes Degani , Ludmila Ferreira Medeiros de França Cardozo , D’Angelo Carlo Magliano and Milena Barcza Stockler-Pinto
Published/Copyright: August 16, 2024
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 21 Issue 3

Abstract

Objectives

To investigate the effects of a Brazil nut-enriched diet on the wall thickness and the left ventricular chamber diameter of the heart, and lipid peroxidation in a CKD-induced model.

Methods

Male Wistar rats at 12 weeks of age were divided into two groups (n=16/group): the Nx group, which underwent 5/6 nephrectomy, and the Sham group, as a control. After 5 weeks, the groups were subdivided according to diet (n=8/group): the Nx and Sham groups received a control diet; the Nx5% and Sham5% groups received a diet enriched with 5 % Brazil nuts for 8 weeks. The left ventricular thickening and chamber diameter were determined. Plasma biochemical parameters were evaluated. Analysis of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activity was performed in the plasma and the left ventricle (LV). LV mRNA expression of nuclear factor-kappa B (NF-κB) and nuclear factor erythroid 2-related factor 2 (Nrf2) was evaluated by reverse transcription-polymerase chain reaction.

Results

The Nx5% group showed a remodeled LV wall with decreased thickness compared to the Nx group (p=0.016). Furthermore, LV TBARS concentration was reduced in the Nx5% group (p=0.0064). In addition, the Nx5% group showed an increase in plasma GPx activity (p=0.0431). No significant results were found concerning the LV mRNA expression of NF-κB and Nrf2 genes.

Conclusions

A Brazil nut-enriched diet decreased LV thickness and LV TBARS concentration and increased GPx activity in a 5/6 nephrectomy experimental model, making it a promising adjuvant therapy to improve antioxidant status and cardiovascular outcomes in chronic kidney disease.

Keywords: chronic kidney disease; left ventricular dysfunction; oxidative stress; Brazil nut
Corresponding author: Michele de Lima Brito, Master’s Degree in Pathology, Laboratory of Experimental Nutrition, Department of Nutrition and Dietetics, Fluminense Federal University, Rua Mário Santos Braga 30, Niterói, RJ 24020-140, Brazil; and Pathology Post Graduate Program, Fluminense Federal University (UFF), Niterói, RJ, Brazil, Email:
Agatha Cristie Menezes and Michele Lima Brito contributed equally to this study.

Funding source: Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do rio de Janeiro

Award Identifier / Grant number: E−26/203.269/2017

  1. Research ethics: The study complied with all current regulations on the use and handling of animals in research. The university’s ethics committee approval number is 956/2017.

  2. Informed consent: Not applicable.

  3. Author contributions: Agatha Cristie Menezes, Michele Lima Brito and Milena Barcza Stockler-Pinto were responsible for the conception, design, interpretation of data, drafting and revising the article providing intellectual content of critical importance to the work described. Patricia Pereira Almeida, Beatriz Oliveira Da Cruz, Nathalia da Silva Costa, Aline D’Avila and Cecilia Castañon Pereira were responsible for data collection, analyses and interpretation of data. Viviane Alexandre Nunes Degani, D’Angelo Carlo Magliano and Ludmila Ferreira Medeiros de França Cardozo were responsible for interpretation of data, revising the article providing intellectual content of critical importance to the work described.

  4. Competing interests: The authors declare no conflicts of interest.

  5. Research funding: This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do rio de Janeiro (FAPERJ) (Process E-26/203.269/2017).

  6. Data availability: We can make it available on request.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/jcim-2024-0123).

Received: 2024-05-01
Accepted: 2024-07-29
Published Online: 2024-08-16

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2024-0123
Keywords for this article
chronic kidney disease; left ventricular dysfunction; oxidative stress; Brazil nut

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Boldine: a narrative review of the bioactive compound with versatile biological and pharmacological potential
  4. Potential anti-cancer activity of Moringa oleifera derived bio-active compounds targeting hypoxia-inducible factor-1 alpha in breast cancer
  5. Research Articles
  6. Phytochemical characterisation and toxicity effect of Tithonia diversifolia (Hemls.) A. Gray leaf extract on fall army worm Spodoptera frugiperda (JE Smith) larvae
  7. Management of wounds in diabetes by administering allicin and quercetin in emulsion form as wound medicine in diabetic rat models
  8. Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of Echinops kebericho Mesfin (Asteraceae) in mice model
  9. A spectrometric analysis of variedly purified cinnabar in a siddha drug – linga chendhooram
  10. Palm oil amends serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats
  11. Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats
  12. Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy
  13. Pharmacognostical characterization, GC-MS profiling, and elemental analysis of Curcuma caesia Roxb. rhizomes for public health
  14. Antibacterial activity of Macrosciadium alatum (M.Bieb.) plant extract
  15. Effect of horticultural therapy on static, dynamic balance and gait speed among institutionalized older adults with cognitive impairment
  16. Anti-hyperlipidemic effects of 500 mg spilanthol (SA3X) supplementation in people with dyslipidemia – a randomized, parallel-group placebo-controlled trial
  17. Potential biomarkers of ASD a target for future treatments: oxidative stress, chemokines, apoptotic, and methylation capacity
  18. Therapeutic ayurvedic interventions for the management of rheumatoid arthritis complicated by adhesive capsulitis – a case report
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