Evaluation of efficacy of Bombax ceiba extract and its major constituent, mangiferin in streptozotocin (STZ)-induced diabetic rats
-
Sushil Bhargava
Abstract
Objectives
Based on the ethno-medicinal use of Bombax ceiba leaf, in the treatment of diabetes, the present study is aimed at evaluation of antidiabetic potential of leaf extract and its major constituent mangiferin.
Methods
Efficacy of hydroalcoholic extract of Bombax ceiba leaf (BCL, 200 and 400 mg/kg body wt.) and mangiferin (MF, 20 mg/kg body wt) was studied in streptozotocin (STZ)-induced diabetic rats and associated complications visually, retinopathy, cardiopathy and nephropathy. After 20 days, serum glucose, lipid profiles, glycol-hemoglobin % (HbA1c%), liver enzymes activity and glycogen content, and histopathology of the pancreas were corroborated. The study was coxswained for development of validated RP-HPLC method for the estimation of MF in BCL.
Results
The results demonstrated significant reductions in the levels of glucose (p<0.001), glycated hemoglobin (HbA1c%, p<0.001), cholesterol, triglycerides and low-density lipoproteins, and concurrent elevation of high density lipoproteins level in the groups administered BCL and MF relative to the controls. It significantly reversed most of the altered metabolic and oxidative stress parameters and histopathological changes. Mangiferin content in BCL was found to be 0.04%w/w.
Conclusions
The anti-diabetic effects of BCL may be attributed to its ability to enhance insulin release, antioxidant and hypolipidemic potential.
Acknowledgments
The Authors are very much thankful to Dr. Rupesh Soni, Asst. Professor in Pharmacology, B. R. Nahata College of Pharmacy, Mandsour (M.P.), India, for assisting us in performing the animal study.
Research funding: None declared.
Author contributions: Both the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Informed consent: Informed consent was obtained from all individuals included in this study.
References
1. Shaw, JE, Sicree, RA, Zimmet, PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4–14. https://doi.org/10.1016/j.diabres.2009.10.007.Suche in Google Scholar
2. Triastuti, A, Park, HJ, Choi, JW. Phaleria macrocarpa suppress nephropathy by increasing renal antioxidant enzyme activity in alloxan-induced diabetic rats. Nat Prod Sci 2009;15:167–72.Suche in Google Scholar
3. Andrew, IR, Scott, B, Clarke, E, Helen, HD, Michael, E, Bell, SC. Microvascular complications in cystic fibrosis-Related Diabetes mellitus: a case report. J Pancreas 2000;14:208–10. https://doi.org/10.6092/1590-8577/378.Suche in Google Scholar
4. Momoh, S, Yusuf, OW, Adamu, MM, Agwu, COC, Atanu, FO. Evaluation of the Phytochemical composition and hypoglycemic activity of methanolic leaves extract of Costus afer in albino rats. Br J Pharmaceut Res 2011;1:1–8. https://doi.org/10.9734/bjpr/2011/237.Suche in Google Scholar
5. Zimmermann, GR, Lehár, J, Keith, CT. Multi-target therapeutics: when the whole is greater than the sum of the parts. Drug Discov Today 2007;12:34–42. https://doi.org/10.1016/j.drudis.2006.11.008.Suche in Google Scholar
6. Chandran, U, Mehendale, N, Tillu, G, Patwardhan, B. Network pharmacology: an emerging technique for natural product drug discovery and scientific research on Ayurveda. Proc Natl Acad Sci India 2015;81:561–8. https://doi.org/10.16943/ptinsa/2015/v81i3/48229.Suche in Google Scholar
7. Bauer, R, Tittel, G. Quality assessment of herbal preparations as a precondition of pharmacological and clinical studies. Phytomedicine 1996;2:193–8. https://doi.org/10.1016/s0944-7113(96)80041-9.Suche in Google Scholar
8. Ong ES. Extraction methods and chemical standardization of botanicals and herbal preparations. J Chromatogr B 2004;812:23–33. https://doi.org/10.1016/j.jchromb.2004.07.041.Suche in Google Scholar
9. World Health Organization. General guidelines for methodologies on research and evaluation of traditional medicine. WHO/EDM/TRM/2000. Geneva, Switzerland: World Health Organization; 2000.Suche in Google Scholar
10. Verma, V, Jalalpure, SS, Sahu, A, Bhardwaj, LK, Prakesh, Y. Bombax ceiba Linn.: pharmacognostical, phytochemistry, ethnobotany, and pharmacology studies. Int J Pharm Sci 2011;1:17–20.Suche in Google Scholar
11. Jain, V, Verma, SK. Assessment of credibility of some folk medicinal claim on Bombax ceiba L. Indian J Tradit Knowl 2014;13:87–94. https://nopr.niscair.res.in/handle/123456789/26029.Suche in Google Scholar
12. Saleem, R, Ahmad, M, Hussain, SA, Qazi, AM, Ahmad, SI, Qazi, MH. Hypotensive, hypoglycaemic and toxicological studies on the flavonol C-glycoside from Bombax ceiba. Planta Med 1999;65:331–4. https://doi.org/10.1055/s-1999-14060.Suche in Google Scholar
13. Bhargava, S, Shah, MB. Evaluation of hypoglycemic activity of different extracts of Bombax ceiba L. leaves. Res J Pharm Technol 2016;9:205–8. https://doi.org/10.5958/0974-360x.2016.00036.6.Suche in Google Scholar
14. Dar, A, Faizi, S, Naqvi, S, Roome, T, UrRehman, SZ, Ali, M, et al. Analgesic and antioxidant activity of mangiferin and its derivatives: the SAR. Biol Pharm Bull 2005;28:596–600. https://doi.org/10.1248/bpb.28.596.Suche in Google Scholar
15. Vieira, TO, Said, A, Aboutabl, E, Azzam, M, Creczynski-Pasa, TB. Antioxidant activity of methanolic extract of Bombax ceiba. Redox Rep 2009;14:1–6. https://doi.org/10.1179/135100009x392485.Suche in Google Scholar
16. Namsa, ND, Tag, H, Mandal, M, Kalita, P, Das, AK. An Ethnobotanical study of traditional anti-inflammatory plants used by the Lohit community of Arunachal Pradesh, India. J Ethnopharmacol 2009;125:234–45. https://doi.org/10.1016/j.jep.2009.07.004.Suche in Google Scholar
17. Rani, P, Khullar, N. Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multidrug resistant Salmonella typhi. Photother Res 2004;18:670–3. https://doi.org/10.1002/ptr.1522.Suche in Google Scholar
18. Girija, K, Chandrika, PU, Lakshman, K, Pruthvi, N. Evaluation of antimicrobial activity of various bark extracts of Bombax malabaricum. Int J Res Pharm Sci 2010;1:199–204. https://doi.org/10.21276/ijlssr.2016.2.5.18.Suche in Google Scholar
19. Hossain, E, Mandal, SC, Gupta, JK. Phytochemical acreening and in-vivo antipyretic activity of the methanol leaf-extract of Bombax malabaricum DC. (Bombacaceae). Trop J Pharmaceut Res 2011;10:55–60. https://doi.org/10.4314/tjpr.v10i1.66541.Suche in Google Scholar
20. Verma, R, Devre, K, Gangrade, T, Gore, S, Gour, S. A pharmacognostic and pharmacological overview on Bombax ceiba. Scholars Acad J Pharm 2014;3:100–7. https://doi.org/10.46624/ajptr.2018.v8.i6.016.Suche in Google Scholar
21. Qin, MJ, Wang, GK, Lin, BB. Study on chemical constituents from leaf of Bombax ceiba (II). Zhong Yao Cai 2014;37:240–2. https://doi.org/10.2174/138955713804999838.Suche in Google Scholar
22. Matkowski, A, Kuś, P, Góralska, E, Woźniak, D. Mangiferin-a bioactive xanthonoid, not only from mango and not just antioxidant. Mini Rev Med Chem 2013;13:439–55. https://doi.org/10.2174/138955713804999838.Suche in Google Scholar
23. Garrido, G, González, D, Delporte, C, Backhouse, N, Quintero, G, Núñez-Sellés, AJ. Analgesic and anti-inflammatory effects of Mangifera indica L. extract (Vimang). Phytother Res 2001;15:18–21.10.1002/1099-1573(200102)15:1<18::AID-PTR676>3.0.CO;2-RSuche in Google Scholar
24. Rani, S, Rahman, K, Sultana, A. Ethnomedicinal and pharmacological activities of mochrus (Bombax ceiba Linn.): an overview. TANG (Humanitas Medicine) 2016;6:1–9. https://doi.org/10.5667/tang.2015.0025.Suche in Google Scholar
25. Harborne, JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. New York: Chapman & Hall; 1980.10.1007/978-94-009-5921-7Suche in Google Scholar
26. Houghton, PJ, Raman, A. Laboratory Handbook for the Fractionation of Natural Extracts. London: Chapman & Hall; 1998 154 p.10.1007/978-1-4615-5809-5_10Suche in Google Scholar
27. ICH Q2 (R1). Validation of Analytical Procedures: Text and Methodology. Geneva: International Conference on Harmonization; 2005.Suche in Google Scholar
28. Graham, ML, Janecek, JL, Kittredge, JA, Hering, BJ, Schuurman, HJ. Streptozotocin-induced diabetic nude mouse model: differences between animals from different sources. Comp Med 2011;61:356–60. 22330251.Suche in Google Scholar
29. Joudaki, R, Setorki, M. The protective effect of Satureja bachtiarica hydroalcoholic extract on streptozotocin‐induced diabetes through modulating glucose transporter and expression and inhibiting oxidative stress. Pharm Biol 2019;57:318–27. https://doi.org/10.1080/13880209.2019.1597131.Suche in Google Scholar
30. Mitra, SK, Gopumadhavan, S, Muralidhar, TS, Anturlikar, SD, Sujatha, MB. Effect of a herbomineral preparation D-400 in streptozotocin induced diabetic rats. J Ethnopharmacol 1996;54:41–6. https://doi.org/10.1016/0378-8741(96)01439-0.Suche in Google Scholar
31. Balamurugan, R, Selvaraj, N, Bobby, Z, Sathiyapriya, V. Increased glycated hemoglobin level in non-diabetic nephrotic children is associated with oxidative stress. Ind J Physiol Pharmacol 2007;51:153–9. 18175659.Suche in Google Scholar
32. Rao, NK, Nammi, S. Antidiabetic and renoprotective effects of the chloroform extract of Terminalia chebula Retz. seeds in streptozotocin-induced diabetic rats. BMC Compl Alternative Med 2006;6:144–7. https://doi.org/10.1186/1472-6882-6-17.Suche in Google Scholar
33. Farswan, M, Mazumder, PM, Percha, V. Protective effect of Cassia glauca L. on the serum glucose and hepatic enzymes level in streptozotocin induced NIDDM in rats. Indian J Pharmacol 2009;41:19–22. https://doi.org/10.4103/0253-7613.48887.Suche in Google Scholar
34. Sharma, A, Bhardwaj, S, Mann, AS, Jain, A, Kharya, MD. Screening method of antioxidant activity: an overview. Pharm Rev 2007;1:232–8.Suche in Google Scholar
35. Goth, L. A simple method for the determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991;196:143–51. https://doi.org/10.1016/0009-8981(91)90067-m.Suche in Google Scholar
36. Madesh, M, Balasubramanian, KA. Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Ind J Biochem Biophys 1998;3:184–8. 9803669.Suche in Google Scholar
37. Wood, LG, Gibson, PG, Gerg, ML. Biomarkers of lipid peroxidation, airway inflammation and asthma. Eur Respir J 2003;21:177–86. https://doi.org/10.1183/09031936.03.00017003a.Suche in Google Scholar
38. Bancroft, JD, Gamble, M. Theory and Practice of Histological Techniques. London, New York and Philadelphia: Churchill Livingstone; 2002.Suche in Google Scholar
39. Ecobichon, DJ. Fixed dose procedure, guideline 420. In. The Basis of Toxicity Testing, 43New York: CRC Press; 1997.Suche in Google Scholar
40. Gianello, P, Steenberghe, MV, Guiot, Y, Goebbels, RM, Saliez, A, Dufrane, D. Streptozotocin induced diabetes in large animals (pigs, primates): role of GLUT2 transporter and β-cell plasticity. Transplantation 2006;81:36–45. https://doi.org/10.1097/01.tp.0000189712.74495.82.Suche in Google Scholar
41. Gilman, G, Rall, TW, Nies, AS, Tayer, P. Goodman and Gilman’s the Pharmacological Basis of Therapeutics. New York: Pergamon Press; 1990.Suche in Google Scholar
42. Saxena, A, Vikram, NK. Role of selected Indian plants in management of type 2 diabetes: a review. J Alternative Compl Med 2004;10:369–78. https://doi.org/10.1089/107555304323062365.Suche in Google Scholar
43. Palsamy, P, Subramanian, S. Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocin-nicotinamide induced experimental diabetic rats. Biomed Pharmacother 2008;62:598–605. https://doi.org/10.1016/j.biopha.2008.06.037.Suche in Google Scholar
44. Guo, F, Huang, C, Liao, X, Wang, Y, He, Y, Feng, R, et al. Beneficial effects of mangiferin on hyperlipidemia in high-fat-fed hamsters. Mol Nutr Food Res 2011;55:1809–18. https://doi.org/10.1002/mnfr.201100392.Suche in Google Scholar
45. Gupta, S, Kataria, M, Gupta, PK, Murganandan, S. Protective role of extracts of neem seeds in diabetes caused by streptozotocin in rats. J Ethnopharmacol 2004;90:185–9. https://doi.org/10.1016/j.jep.2003.09.024.Suche in Google Scholar
46. Mohamed, K, Bierhaus, A, Schiekofer, S, Tritschler, H, Ziegler, R, Nawroth, PP. The role of oxidative stress and NF-κB activation in late diabetic complications. Biofactors 1999;10:157–67. https://doi.org/10.1002/biof.5520100211.Suche in Google Scholar
© 2020 Walter de Gruyter GmbH, Berlin/Boston
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Artikel in diesem Heft
- Frontmatter
- Reviews
- The effect of Nigella sativa on oxidative stress and inflammatory biomarkers: a systematic review and meta-analysis
- Effect of fennel on primary dysmenorrhea: a systematic review and meta-analysis
- Raising burden of non-communicable diseases: importance of integrating Yoga and Naturopathy at primary care level
- Research Articles Basic
- Marketing and legal status of phytomedicines and food supplements in Morocco
- Hydrogen sulfide as a mediator of endothelium-dependent relaxation evoked by Moringa oleifera leaf extract in mesenteric arterial beds isolated from L-NAME hypertensive rats
- Acute and sub-acute toxicity assessment of aqueous leaves extract of Crassocephalum crepidioides (Asteraceae) in Wistar rats
- Zingerone improves the immune responses in an animal model of breast cancer
- Evaluation of efficacy of Bombax ceiba extract and its major constituent, mangiferin in streptozotocin (STZ)-induced diabetic rats
- Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential
- Ameliorative effect of morin on dutasteride-tamsulosin-induced testicular oxidative stress in rat
- The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus
- Influence of irisin on diet-induced metabolic syndrome in experimental rat model
- Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia
- Research Articles Clinical
- Functional status in adhesive capsulitis – yoga vs. physiotherapy: a quasi experimental study
- Okada Purifying Therapy in combination with duloxetine vs. duloxetine alone in patients with TMD and fibromyalgia: a randomized clinical study
- The FIFA 11+ does not alter performance in amateur female basketball players—a randomized control trial
- Body composition, phenotype and central obesity indices in Egyptian women with non-alcoholic fatty liver disease
- Immediate effect of interscapular cupping on blood pressure, oxygen saturation, pulse rate and chest expansion in sedentary smoker students
- The effects of an olive-oil massage on hemodialysis patients suffering from fatigue at a hemodialysis unit in southern India – a randomized controlled trial
- Effects of epigallocatechin-3-gallate of Camellia sinensis leaves on blood pressure, lipid profile, atherogenic index of plasma and some inflammatory and antioxidant markers in type 2 diabetes mellitus patients: a clinical trial
- The effect of practicing yoga during pregnancy on labor stages length, anxiety and pain: a randomized controlled trial
- Effect of lumbar stabilization exercises and thoracic mobilization with strengthening exercises on pain level, thoracic kyphosis, and functional disability in chronic low back pain
- A prospective controlled study on Ramadan fasting in the healthy young males in summer in Germany: effect on cytokines
- Tualang honey versus steroid impregnated nasal dressing following endoscopic sinus surgery: a randomized controlled trial
- Short Communications
- Improved anti-arthritic activity of ginger extract, a traditional medicine, using novel drug delivery approach
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