The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus
-
Sangeeta V. Alagasamy
,
Nelson Jeng-Yeou Chear
Abstract
Objectives
Mesua ferrae, from the family of Calophyllaceae, is traditionally used for the treatment of piles, fever and renal disorders. The present study was aimed to examine the antibacterial compounds from the leaves of M. ferrae and their β-lactam antibiotic potentiate activities against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA).
Methods
Stigmasterol (1) and β-caryophyllene oxide (2) were isolated from the n-hexane fraction of the leaves of M. ferrae using a bioassay-guided fractionation approach.
Results
The isolated compounds displayed anti-Staphylococcus and anti-MRSA activities. It is worth to note that both compounds demonstrated synergism with β-lactam antibiotics against S. aureus and MRSA. Gas chromatography–mass spectrometry (GC–MS) analysis indicated the n-hexane fraction was dominated by triterpenes and sesquiterpenes, suggesting the total antibacterial activity exhibited by the fraction.
Conclusion
Based on the findings, it could conclude that M. ferrae is a promising natural source for the discovery of new anti-MRSA lead compounds.
Funding source: Universiti Sains Malaysia
Award Identifier / Grant number: 1001/CDADAH/815096
Funding source: Universiti Sains Malaysia
Research funding: This study was supported by Universiti Sains Malaysia under the Research University Grant (1001/CDADAH/815096). Nelson Jeng-Yeou Chear and Dinesh Sangarran Ramachandram are the recipients of USM Fellowship Scheme funded by the Institute of Postgraduate Studies, Universiti Sains Malaysia. Ai-Fein T. Ching-Ga is the recipient of USM Graduate-Assistant Scheme funded by the Institute of Postgraduate Studies, Universiti Sains Malaysia.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: All contributing authors declare no conflicts of interest.
References
1. Chopra, RN, Nayar, SL, Chopra, IC. Glossary of Indian medicinal plants. Council of Scientific and Industrial Research; 1956: 166 p.Search in Google Scholar
2. Sharma, A, Sharma, S, Parashar, B. Mesua ferrae linn – a review of the Indian medical herb. Sys Rev Pharm 2017;8:19–23.10.5530/srp.2017.1.5Search in Google Scholar
3. Usha, S, Shanta, M, Sharma, HP. Pharmacognostic studies on the flower of Mesua ferrea L. Proc Indian Acad Sci 1982;91:211–2.10.1007/BF03167125Search in Google Scholar
4. Mazumder, R, Dastidar, SG, Basu, SP, Mazumder, A, Singh, SK. Antibacterial potentiality of Mesua ferrea Linn flowers. Phytother Res 2004;18:824–6, https://doi.org/10.1002/ptr.1572.Search in Google Scholar PubMed
5. Abbas, AM, Abu, SM, Bhuiyan, MSA, Sohel, FI, Sarmina, YM. Antimicrobial screening of Cassia fistula and Mesua ferrea. J Med Sci 2004;4:24–9.10.3923/jms.2004.24.29Search in Google Scholar
6. Aruldass, CA, Marimuthu, MM, Ramanathan, S, Mansor, SM, Murugaiyah, V. Effects of Mesua ferrea leaf and fruit extracts on growth and morphology of S. aurues. Microse Microanal 2013;18:1–7.10.1017/S1431927612013785Search in Google Scholar
7. Adewale, AI, Mirghani, MES, Muyibi, SA, Daoud, JI, Abimbola, MM. Disinfection studies of Nahar (Mesua ferrea) seed kernel oil using pour plate method. Afr J Biotechnol 2011;10:18749–54.10.5897/AJB11.2747Search in Google Scholar
8. Asif, M, Jafari, SF, Iqbal, Z, Revadigar, V, Oon, CE, Majid, ASA, et al.. Ethnobotanical and phytopharmacological attributes of Mesua ferrea: a mini review. J Appl Pharmaceut Sci 2017;7:242–51.Search in Google Scholar
9. Bandow, JE, Brotz, H, Leichert, L, Labischinski, H, Hecker, M. Proteomic approach to understanding antibiotic action. Antimicrob Agents Chemother 2003;47:948–55, https://doi.org/10.1128/aac.47.3.948-955.2003.Search in Google Scholar PubMed PubMed Central
10. Li, B, Webster, TJ. Bacteria antibiotic resistance: new challenges and opportunities for implant-associated orthopaedic infections. J Orthop Res 2018;36:22–32.10.1002/jor.23656Search in Google Scholar PubMed PubMed Central
11. Casal, M, Vaquero, M, Rinder, H, Tortoli, E, Grosset, J, Rusch-Gerdes, S, et al.. A case-control study for multidrug-resistant tuberculosis: risk factors in four European countries. Microb Drug Resist 2005;11:62–7, https://doi.org/10.1089/mdr.2005.11.62.Search in Google Scholar PubMed
12. Chambers, HF. The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 2001;7:178–82, https://doi.org/10.3201/eid0702.010204.Search in Google Scholar PubMed PubMed Central
13. Eloff, JN. A sensitive and quick microplate method to determine the minimum inhibitory concentration of plant extracts for bacteria. Planta Med 1998;64:711–3, https://doi.org/10.1055/s-2006-957563.Search in Google Scholar PubMed
14. Chear, NJY, Khaw, KY, Murugaiyah, V, Lai, CS. Cholinesterase inhibitory activity and chemical constituents of Stenochlaena palustris fronds at two different stages of maturity. J Food Drug Anal 2016;24:358–66, https://doi.org/10.1016/j.jfda.2015.12.005.Search in Google Scholar PubMed
15. Carradori, S, Chimenti, P, Fazzari, M, Granese, A, Angiolella, L. Antimicrobial activity, synergism and inhibition of germ tube formation by Crocus sativus-derived compounds against Candida spp. J Enzym Inhib Med Chem 2016;31:189–93, https://doi.org/10.1080/14756366.2016.1180596.Search in Google Scholar PubMed
16. Hu, ZQ, Zhao, WH, Yoda, Y, Asano, N, Hara, Y, Shimamura, T. Additive, indifferent and antagonistic effects in combinations of epigallocatechin gallate with 12 non-β-lactam antibiotics against methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 2002;50:1051–4, https://doi.org/10.1093/jac/dkf250.Search in Google Scholar PubMed
17. Hui, MX. Chemical constituents and biological activities of Garcinia cuneifolia, Mesua beccariana and Mesua ferrea [PhD thesis]. Universiti Putra Malaysia; 2005.Search in Google Scholar
18. Choudhury, S, Ahmed, R, Barthel, A, Leclercq, PA. Volatile oils of Mesua ferrae (L.) from Assam, India. J Essent Oil Res 1998;10:497–501, https://doi.org/10.1080/10412905.1998.9700955.Search in Google Scholar
19. Keawsaard, S, Kongtaweelert, S. Antioxidant, antibacterial, anticancer ractivities and chemical constituents of the essential oil from Mesua ferrea leaves. Chiang Mai J Sci 2012;39:455–63.Search in Google Scholar
20. French, GL. Bactericidal agents in the treatment of MRSA infections—the potential role of daptomycin. J Antimicrob Chemother 2006;58:1107–17, https://doi.org/10.1093/jac/dkl393.Search in Google Scholar PubMed
21. Satish, KP, Moellering, RC, Eliopoulus, GM. Antimicrobial combinations in: Lorian Ved. Antibiot Lab Med 2005:365–440.Search in Google Scholar
22. Hemaiswarya, S, Kruthiventi, AK, Doble, M. Synergism between natural products an antibiotics against infectious diseases. Phytomedicine 2008;15:639–52, https://doi.org/10.1016/j.phymed.2008.06.008.Search in Google Scholar PubMed
© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- The effect of Nigella sativa on oxidative stress and inflammatory biomarkers: a systematic review and meta-analysis
- Effect of fennel on primary dysmenorrhea: a systematic review and meta-analysis
- Raising burden of non-communicable diseases: importance of integrating Yoga and Naturopathy at primary care level
- Research Articles Basic
- Marketing and legal status of phytomedicines and food supplements in Morocco
- Hydrogen sulfide as a mediator of endothelium-dependent relaxation evoked by Moringa oleifera leaf extract in mesenteric arterial beds isolated from L-NAME hypertensive rats
- Acute and sub-acute toxicity assessment of aqueous leaves extract of Crassocephalum crepidioides (Asteraceae) in Wistar rats
- Zingerone improves the immune responses in an animal model of breast cancer
- Evaluation of efficacy of Bombax ceiba extract and its major constituent, mangiferin in streptozotocin (STZ)-induced diabetic rats
- Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential
- Ameliorative effect of morin on dutasteride-tamsulosin-induced testicular oxidative stress in rat
- The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus
- Influence of irisin on diet-induced metabolic syndrome in experimental rat model
- Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia
- Research Articles Clinical
- Functional status in adhesive capsulitis – yoga vs. physiotherapy: a quasi experimental study
- Okada Purifying Therapy in combination with duloxetine vs. duloxetine alone in patients with TMD and fibromyalgia: a randomized clinical study
- The FIFA 11+ does not alter performance in amateur female basketball players—a randomized control trial
- Body composition, phenotype and central obesity indices in Egyptian women with non-alcoholic fatty liver disease
- Immediate effect of interscapular cupping on blood pressure, oxygen saturation, pulse rate and chest expansion in sedentary smoker students
- The effects of an olive-oil massage on hemodialysis patients suffering from fatigue at a hemodialysis unit in southern India – a randomized controlled trial
- Effects of epigallocatechin-3-gallate of Camellia sinensis leaves on blood pressure, lipid profile, atherogenic index of plasma and some inflammatory and antioxidant markers in type 2 diabetes mellitus patients: a clinical trial
- The effect of practicing yoga during pregnancy on labor stages length, anxiety and pain: a randomized controlled trial
- Effect of lumbar stabilization exercises and thoracic mobilization with strengthening exercises on pain level, thoracic kyphosis, and functional disability in chronic low back pain
- A prospective controlled study on Ramadan fasting in the healthy young males in summer in Germany: effect on cytokines
- Tualang honey versus steroid impregnated nasal dressing following endoscopic sinus surgery: a randomized controlled trial
- Short Communications
- Improved anti-arthritic activity of ginger extract, a traditional medicine, using novel drug delivery approach
- Efficacy of fenugreek seed extract on men's psychological and physical health: a randomized placebo-controlled double-blind clinical trial
Articles in the same Issue
- Frontmatter
- Reviews
- The effect of Nigella sativa on oxidative stress and inflammatory biomarkers: a systematic review and meta-analysis
- Effect of fennel on primary dysmenorrhea: a systematic review and meta-analysis
- Raising burden of non-communicable diseases: importance of integrating Yoga and Naturopathy at primary care level
- Research Articles Basic
- Marketing and legal status of phytomedicines and food supplements in Morocco
- Hydrogen sulfide as a mediator of endothelium-dependent relaxation evoked by Moringa oleifera leaf extract in mesenteric arterial beds isolated from L-NAME hypertensive rats
- Acute and sub-acute toxicity assessment of aqueous leaves extract of Crassocephalum crepidioides (Asteraceae) in Wistar rats
- Zingerone improves the immune responses in an animal model of breast cancer
- Evaluation of efficacy of Bombax ceiba extract and its major constituent, mangiferin in streptozotocin (STZ)-induced diabetic rats
- Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential
- Ameliorative effect of morin on dutasteride-tamsulosin-induced testicular oxidative stress in rat
- The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus
- Influence of irisin on diet-induced metabolic syndrome in experimental rat model
- Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia
- Research Articles Clinical
- Functional status in adhesive capsulitis – yoga vs. physiotherapy: a quasi experimental study
- Okada Purifying Therapy in combination with duloxetine vs. duloxetine alone in patients with TMD and fibromyalgia: a randomized clinical study
- The FIFA 11+ does not alter performance in amateur female basketball players—a randomized control trial
- Body composition, phenotype and central obesity indices in Egyptian women with non-alcoholic fatty liver disease
- Immediate effect of interscapular cupping on blood pressure, oxygen saturation, pulse rate and chest expansion in sedentary smoker students
- The effects of an olive-oil massage on hemodialysis patients suffering from fatigue at a hemodialysis unit in southern India – a randomized controlled trial
- Effects of epigallocatechin-3-gallate of Camellia sinensis leaves on blood pressure, lipid profile, atherogenic index of plasma and some inflammatory and antioxidant markers in type 2 diabetes mellitus patients: a clinical trial
- The effect of practicing yoga during pregnancy on labor stages length, anxiety and pain: a randomized controlled trial
- Effect of lumbar stabilization exercises and thoracic mobilization with strengthening exercises on pain level, thoracic kyphosis, and functional disability in chronic low back pain
- A prospective controlled study on Ramadan fasting in the healthy young males in summer in Germany: effect on cytokines
- Tualang honey versus steroid impregnated nasal dressing following endoscopic sinus surgery: a randomized controlled trial
- Short Communications
- Improved anti-arthritic activity of ginger extract, a traditional medicine, using novel drug delivery approach
- Efficacy of fenugreek seed extract on men's psychological and physical health: a randomized placebo-controlled double-blind clinical trial
