Influence of irisin on diet-induced metabolic syndrome in experimental rat model
-
Dalia Medhat
,
Mona A. El-Bana
Abstract
Objective
To evaluate the influence of irisin on the experimental paradigm of non-alcoholic fatty liver (NAFL) as a part of MetS cluster.
Methods
Forty male albino rats were divided into four groups; normal control, standard diet + irisin, high carbohydrate and fat diet (HCHF), and HCHF + irisin. After the experimental period, levels of fasting blood sugar (FBS), insulin, lipid profile, kidney functions, salusin-alpha (Sal-α), adropin, and retinol-binding protein-4 (RBP-4) were evaluated. Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α) expression in skeletal muscle was evaluated by quantitative real-time PCR. Aorta, liver, pancreas, and skeletal muscle tissue samples were prepared for histopathological examination.
Results
Rats administrated HCHF showed elevated levels of FBS, lipid profile, kidney functions, RBP-4, and downregulation of PGC-1α expression along with a decline in levels of insulin, Sal-α, and adropin while administration of irisin significantly attenuated these levels.
Conclusions
Irisin as based therapy could emerge as a new line of treatment against MetS and its related diseases.
Funding source: National Research Centre
Award Identifier / Grant number: 11010131
Acknowledgement
Authors are grateful to National Research Centre, Egypt for supporting this work.
Research funding: This work was supported by the research project of the National Research Centre (Project ID: 11010131).
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors declare that there is no conflict of interest.
Ethical approval: This study was approved by the Institutional Animal Ethics Committee of NRC, Giza, Egypt, and under the permit of the Ethics Committee of NRC No.16/355.
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- Frontmatter
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- Hydrogen sulfide as a mediator of endothelium-dependent relaxation evoked by Moringa oleifera leaf extract in mesenteric arterial beds isolated from L-NAME hypertensive rats
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- Evaluation of efficacy of Bombax ceiba extract and its major constituent, mangiferin in streptozotocin (STZ)-induced diabetic rats
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- A prospective controlled study on Ramadan fasting in the healthy young males in summer in Germany: effect on cytokines
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