Startseite The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect
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The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect

  • Zineb Choukairi EMAIL logo , Tahar Hazzaz , Manuel Ferrandez José und Taoufiq Fechtali
Veröffentlicht/Copyright: 25. Dezember 2020

Abstract

Background

Rosmarinus officinalis L. (Rosemary) and Salvia officinalis L. (Sage) are two Mediterranean species growing spontaneously in some area in Morocco. They are used in traditional and complementary medicine to treat numerous disorders. The aim of this work was to assess the in vitro antitumoral effect of the methanolic total extract prepared from rosemary and sage on human glioblastoma cell line (42 GMBA), conjointly with their antioxidant activity.

Methods

The accelerated solvent extractor was used to obtain the total extract of the studied plants. The antitumor activity was performed using the microculture tetrazolium cytotoxique assay while the antioxidant effect was evaluated using the ferric reducing antioxidant power (FRAP), and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay.

Results

Our results show that the total extract of R.O and S.O have a cytotoxic effect on glioblastoma but not on cortical neurons. On the other hand, the results obtained in the FRAP and DPPH tests show a dose-dependent antioxidant activity correlated with an important level of phenols and flavonoids.

Conclusion

Rosmarinus officinalis L. and Salvia officinalis L. were found to have an antitumoral activity which may be linked, probably, to an antioxidant process.

Acknowledgments

This work was supported by the grant of the Moroccan ministry and Erasmus Grant.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] Quezel P, Et Santa S. Nouvelle flore de l’Algérie et des régions désertiques méridoniales, Tome II ed. Paris: CNRS, 1963.Suche in Google Scholar

[2] Spichiger RE. Botanique Systématique des plantes à fleurs: une approche phylogénétique nouvelle des angiospermes des régions tempérées et tropicales. PPUR presses polytechnique, 2002:328.Suche in Google Scholar

[3] Meikle RD. Flora of Cyprus, 2. Kew:The BenthamMoxon Trust, Royal Botanic Gardens, 1985.Suche in Google Scholar

[4] Moore J, Yousef M, Tsiani E. Anticancer effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary extract polyphenols. Nutrients 2016;8:E731.10.3390/nu8110731Suche in Google Scholar PubMed PubMed Central

[5] Ghorbani A, Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. J Traditional Complementary Med 2017 January 13;7(4):433–40.10.1016/j.jtcme.2016.12.014Suche in Google Scholar PubMed PubMed Central

[6] Ulbricht C, Abrams TR, Brigham A, Ceurvels J, Clubb J, Curtiss W, et al. An evidence-based systematic review of Rosemary (Rosmarinus officinalis) by the natural standard research collaboration. J Diet 2010;7:351–413.10.3109/19390211.2010.525049Suche in Google Scholar PubMed

[7] Ferlemi AV, Katsikoudi A, Kontogianni VG, Kellici TF, Iatrou G, Lamari FN, et al. Rosemary tea consumption results to anxiolytic- and anti-depressant like behavior of adult male mice and inhibits all cerebral area and liver cholinesterase activity; phytochemical investigation and in silico studies. Chem Biol Interact 2015;237:47–57.10.1016/j.cbi.2015.04.013Suche in Google Scholar PubMed

[8] McCaffrey R, Thomas DJ, Kinzelman AO. The effects of lavender and rosemary essential oils on test-taking anxiety among graduate nursing students. Holist Nurs Pract 2009;23:88–93.10.1097/HNP.0b013e3181a110aaSuche in Google Scholar PubMed

[9] Machado DG, Cunha MP, Neis VB, Balen GO, Colla A, Bettio LE, et al. Antidepressant-like effects of fractions, essential oil, carnosol and betulinic acid isolated from Rosmarinus officinalis L. Food Chem 2013;136:999–1005.10.1016/j.foodchem.2012.09.028Suche in Google Scholar PubMed

[10] Kennedy D, Pace S, Haskell C, Okello E, Milne A, Scholey A. Effect of cholinesterase inhibitory sage (Salvia officinalis) on mood, anxiety and performance on a psychological stressor battery. Neuropsychopharmacology 2006;31:845–52.10.1038/sj.npp.1300907Suche in Google Scholar PubMed

[11] Santana-Méridas O, Polissiou M, Izquierdo-Melero ME, Astraka K, Tarantilis PA, Herraiz-Peñalver D, et al. Polyphenol composition, antioxidant and bioplaguicide activities of the solid residue from hydrodistillation of Rosmarinus officinalis L. Ind Crops Prod 2014;59:125–34.10.1016/j.indcrop.2014.05.008Suche in Google Scholar

[12] Horváthová E, Srančíková A, Regendová-Sedláčková E, Melušová M, Meluš M, Netriová J, et al. Enriching the drinking water of rats with extracts of Salvia officinalis and Thymus vulgaris increases their resistance to oxidative stress. Mutagenesis 2016;31:51–9.10.1093/mutage/gev056Suche in Google Scholar

[13] Pedro Dalila FN, Ramos Alice A, Lima Cristovao F, Baltazar F, Pereira-Wilson C. Colon cancer chemoprevention by sage tea drinking: decreased DNA damage and cell proliferation. Phytother 2016;30:298–305.10.1002/ptr.5531Suche in Google Scholar PubMed

[14] Shahneh FT, Valiyari S, Baradaran B, Abdolalizadeh J, Bandehagh A, Azadmehr A, et al. Inhibitory and cytotoxic activities of Salvia officinalis L. Extract on human lymphoma and leukemia cells by induction of apoptosis. Adv Pharm Bull 2013;3:51–5.Suche in Google Scholar

[15] Yi W, Wetzstein H. Anti-tumorigenic activity of five culinary and medicinal herbs grown under greenhouse conditions and their combination effects. J Sci Food Agric 2011;91:1849–54.10.1002/jsfa.4394Suche in Google Scholar PubMed

[16] Ostrom QT, Gittleman H, Liao P, Rouse C, Chen Y, Dowling J, et al. CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2007–2011. Neuro Oncol 2014;16:iv1–iv63.10.1093/neuonc/nou223Suche in Google Scholar

[17] Martins-Gomes A. Nouvelles approches thérapeutiques pour le traitement du glioblastome: exemple du développement d’une protéine pro-apoptotique. Sciences pharmaceutiques. 2012Suche in Google Scholar

[18] Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P. The 2007 WHO classification of tumors of the central nervous system. Acta Neuropathol 2007;114:97–109.10.1007/s00401-007-0243-4Suche in Google Scholar

[19] Stupp R, Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med 2005;352:987–96.10.1056/NEJMoa043330Suche in Google Scholar

[20] Arvold ND, Reardon DA. Treatment options and outcomes for glioblastoma in the elderly patient. Clin Interv Aging 2014;9:357–67.10.2147/CIA.S44259Suche in Google Scholar

[21] Thakkar JP, Dolecek TA, Horbinski C, Ostrom QT, Lightner DD, Barnholtz-Sloan JS, et al. Epidemiologic and molecular prognostic review of glioblastoma. Cancer Epidemiol Biomarkers Prev 2014;23:1985–96.10.1158/1055-9965.EPI-14-0275Suche in Google Scholar

[22] Szabo AT, Carpentier AF. Immunotherapy in human glioblastoma. Rev Neurol (Paris) 2011;167:668–72.10.1016/j.neurol.2011.07.002Suche in Google Scholar

[23] Kosanić M, Ranković B, Stanojković T, Vasiljević P, Manojlović N. Biological activities and chemical composition of lichens from Serbia. Excli J 2014;13:1226–38.Suche in Google Scholar

[24] Oyaizu M. Studies on Products of Browning Reaction—Antioxidative Activities of Products of Browning Reaction. Prepared from Glucosamine. Jpn J Nutr 1986;44:307–15.10.5264/eiyogakuzashi.44.307Suche in Google Scholar

[25] Naczk M, Amarowicz R, Zadernowski R, Pegg RB, Shahidi F. Antioxidant activity of crude phenolic extracts from wild blueberry leaves. Pol J Food Nutr Sci 2003;12:166–9.Suche in Google Scholar

[26] Singleton VL, Rossi JA. Colorimetry of total phenolics with phosph omolyb dicphos photungstic acid reagents. Am J Enol Vitic 1965;16:144–58.10.5344/ajev.1965.16.3.144Suche in Google Scholar

[27] Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet J, Luyck M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 2000;72:35–40.10.1016/S0378-8741(00)00196-3Suche in Google Scholar

[28] Sertel S, Eichhorn T, Plinkert PK, Efferth T. Anticancer activity of Salvia officinalis essential oil against HNSCC cell line (UMSCC1). HNO 2011;59:1203–8.10.1007/s00106-011-2274-3Suche in Google Scholar PubMed

[29] González-Vallinas M, Molina S, Vicente G, la Cueva A, Vargas T, Santoyo S, et al. Antitumor effect of 5-fluorouracil is enhanced by rosemary extract in both drug sensitive and resistant colon cancer cells. Pharmacological Res 2013;72:61–8.10.1016/j.phrs.2013.03.010Suche in Google Scholar PubMed

[30] Kontogianni VG, Tomic G, Nikolic I, Nerantzaki AA, Sayyad N, Stosic-Grujicic S, et al. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chemistry 2013;1363:120–9.10.1016/j.foodchem.2012.07.091Suche in Google Scholar

[31] Xu Y, Xu G, Liu L, Xu D, Liu J. Anti-invasion effect of rosmarinic acid via the extracellular signal-regulated kinase and oxidation–reduction pathway in Ls174-T cells. J Cell Biochem 2010;111:370–9.10.1002/jcb.22708Suche in Google Scholar

[32] Jeshvaghani ZA, Rahimmalek M, Talebi M, Amir S, Goli H. Comparison of total phenolic content and antioxidant activity in different Salvia species using three model systems. Ind Crops Prod 2015;77:409–14.10.1016/j.indcrop.2015.09.005Suche in Google Scholar

[33] Takayama C, Meirade-Faria F, Ana Cristina Alves DA, Josédunder R, PauloManzo L, Socca EA, et al. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat. Asian Pac J Trop Biomed 2016;6:677–81.10.1016/j.apjtb.2015.09.027Suche in Google Scholar

[34] Zaouali Y, Bouzaine T, Boussaid M. Essential oils composition in two Rosmarinus officinalis L. varieties incidence for antimicrobial and antioxidant activities. Food Chem Toxicol 2010;48:3144–52.10.1016/j.fct.2010.08.010Suche in Google Scholar

[35] Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med 2010;49:1603–16.10.1016/j.freeradbiomed.2010.09.006Suche in Google Scholar

[36] Manning P, Calum J, Jonathan M, Cooper, Hillhouse EW. Direct, real-time sensing of free radical production by activated human glioblastoma cells. Free Radic Biol Med 1998;24:1304–9.10.1016/S0891-5849(97)00455-3Suche in Google Scholar

Received: 2018-10-03
Accepted: 2019-11-26
Published Online: 2020-12-25

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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