Home Medicine Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage
Article
Licensed
Unlicensed Requires Authentication

Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage

  • Hnia Chograni EMAIL logo , Leila Riahi , Samir Dhahri , Olfa Ezzine , Hanen Chakroun and Chokri Messaoud
Published/Copyright: March 31, 2020

Abstract

Background

Eucalyptus essential oils and extracts are used since the ancient times in alternative medicine. The 1,8-cineole is the most significant constituent of Eucalyptus essential oil, while phenolic contents define the value of eucalyptus extracts. Based on the last considerations, interspecific variability of 1,8-cineole content, phenolics and antioxidant potentials among nine Eucalyptus taxa growing under the sub-humid bioclimate stage of Tunisia was investigated.

Methods

Essential oil profiling was assessed based on gas chromatography-mass spectrometry analysis. Total phenolic and flavonoid contents were assessed using Folin–Ciocalteau and aluminium chloride colorimetric methods, respectively. The antioxidant ability of Eucalyptus volatile metabolites and extracts was achieved based on two test systems namely DPPH and FRAP assays.

Results

Qualitative and quantitative variations in the composition of essential oils according to the studied taxon were shown. The total phenolic and flavonoid contents varied also significantly among the investigated samples. Based on the obtained results, the species Eucalyptus sideroxylon exhibits the highest 1,8-cineole content (76.24 ± 0.86), total phenolics and flavonoids contents (38.5 ± 1.4 mg GAE /g DW and 18.6 ± 0.3 mg RE /g DW, respectively). Moreover this species highlighted the highest free radical-scavenging ability and ferric reducing power for both essential oil and methanolic extracts. Chemometric multivariate analysis showed the classification of the nine studied taxa to three clusters.

Conclusions

The pattern of 1,8-cineole concentration, phenolic and flavonoid contents for the studied nine Eucalyptus species and hybrid showed E. sideroxylon species as the potential candidate for further improvement strategies regarding the production of eucalyptus essential oils and phenolics with high quality for pharmaceutical industry.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] Rockwood LD, Rudie WA, Ralph SA, Zhu YJ, Winandy EJ. Energy product options for Eucalyptus species grown as short rotation woody crops. Int J Mol Sci 2008;9:1361–78.10.3390/ijms9081361Search in Google Scholar PubMed PubMed Central

[2] Pirralho M, Flores D, Sousa VB, Quilhó T, Knapic S, Pereira H. Evaluation on paper making potential of nine Eucalyptus species based on wood anatomical features. Ind Crops Prod 2014;54:327–34.10.1016/j.indcrop.2014.01.040Search in Google Scholar

[3] Dhahri S, Lieutier F, Charfi Cheikhrouha F, Ben Jamaa ML. Distribution, preference and performance of Phoracantha recurva and Phoracantha semipunctata (Coleoptera cerambycidae) on various Eucalyptus species in Tunisia. REDIA 2016;XCIX:83–95.10.19263/Redia-99.16.12Search in Google Scholar

[4] Konoshima T, Takasaki M. Chemistry and bioactivity of the non-volatile constituents of Eucalyptus. In: JJ Coppen, editor. Eucalyptus: The Genus Eucalyptus. New York: Taylor & Francis, London, 2002:269–90.10.4324/9780203219430_chapter_12Search in Google Scholar

[5] Menale B, De Castro O, Cascone C, Muoio R. Ethnobotanical investigation on medicinal plants in the Vesuvio National Park (Campania, Southern Italy). J Ethnopharmacol 2016;192:320–49.10.1016/j.jep.2016.07.049Search in Google Scholar PubMed

[6] Salehi B, Sharifi-Rad J, Quispe C, Llaique H, Villalobos M, Smeriglio A, et al. Insights into Eucalyptus genus chemical constituents, biological activities and health-promoting effects. Trends Food Sci Tech. DOI: https://doi.org/10.1016/j.tifs.2019.08.003.Search in Google Scholar

[7] Romani A, Larramendi A, Yáñez R, Cancela Á, Sánchez Á, Teixeira JA, et al. Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels. Ind Crops Prod 2019;132:327–35.10.1016/j.indcrop.2019.02.040Search in Google Scholar

[8] Batish DR, Singh HP, Kohli RK, Kaur S. Eucalyptus essential oil as a natural pesticide. Forest Ecol Manag 2008;256:2166–74.10.1016/j.foreco.2008.08.008Search in Google Scholar

[9] Tolba H, Moghrani H, Benelmouffok A, Kellou D, Maachi R. Essential oil of Algerian Eucalyptus citriodora: chemical composition, antifungal activity Huile essentielle d’Eucalyptus citriodora: composition chimique et activité antifongique. J Mycol Méd 2015;25:e128–e33.10.1016/j.mycmed.2015.10.009Search in Google Scholar PubMed

[10] Dhakad AK, Pandey VV, Beg S, Rawat JM, Singh A. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review. J Sci Food Agric 2018;98:833–48.10.1002/jsfa.8600Search in Google Scholar PubMed

[11] Tariq S, Wani S, Rasool W, Shafi K, Bhat MA, Prabhakar A, et al. Comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microb Pathog 2019;134:103580.10.1016/j.micpath.2019.103580Search in Google Scholar PubMed

[12] Goodger JQ, Woodrow IE. Selection gains for essential oil traits using micropropagation of Eucalyptus polybractea. Forest Ecol Manag 2008;255:3652–8.10.1016/j.foreco.2008.03.006Search in Google Scholar

[13] Haddad M, Zein S, Shahrour H, Hamadeh K, Karaki N, Kanaan H. Antioxidant activity of water-soluble polysaccharide extracted from Eucalyptus cultivated in Lebanon. Asian Pac J Trop Biomed 2017;7:157–60.10.1016/j.apjtb.2016.11.024Search in Google Scholar

[14] Juergens LJ, Tuleta I, Stoeber M, Racké K, Juergens UR. Regulation of monocyte redox balance by 1,8-cineole (eucalyptol) controls oxidative stress and pro-inflammatory responses in vitro: a new option to increase the antioxidant effects of combined respiratory therapy with budesonide and formoterol. Synergy 2018;7:1–9.10.1016/j.synres.2018.05.001Search in Google Scholar

[15] Zhang Y, Liu Y, Li Q, Wang X, Zheng X, Yang B, et al. 1,8-cineole decreases neuropathic pain probably via a mechanism mediating P2X3 receptor in the dorsal root ganglion. Neurochem Int 2018;121:69–74.10.1016/j.neuint.2018.09.007Search in Google Scholar PubMed

[16] Worth H, Dethlefsen U. Patients with asthma benefit from concomitant therapy with cineole: a placebo-controlled, double blind trial. J Asthma 2012;49:849–53.10.3109/02770903.2012.717657Search in Google Scholar PubMed

[17] Chograni H, Riahi L, Zaouali Y, Boussaid M. Polyphenols, flavonoids, antioxidant activity in leaves and flowers of Tunisian Globularia alypum L. (Globulariaceae). Afr J Ecol 2013;51:343–7.10.1111/aje.12041Search in Google Scholar

[18] Riahi L, Elferchichi M, Ghazghazi H, Jebali J, Ziadi S, Aouadhi C, et al. Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia. Ind Crops Prod 2013;49:883–9.10.1016/j.indcrop.2013.06.032Search in Google Scholar

[19] Vuong QV, Chalmers AC, Jyoti Bhuyan D, Bowyer MC, Scarlett CJ. Botanical, phytochemical, and anticancer properties of the Eucalyptus species. Chem Biodivers 2015;12:907–24.10.1002/cbdv.201400327Search in Google Scholar PubMed

[20] Bhuyan DJ, Vuong QV, Chalmers AC, van Altena IA, Bowyer MC, Scarlett CJ. Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves. S Afr J Bot 2017;112:180–5.10.1016/j.sajb.2017.05.030Search in Google Scholar

[21] Gonzalez-Burgos E, Liaudanskas M, Viskelis J, Zvikas V, Janulis V, Gomez-Serranillos MP. Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves. J Food Drug Anal 2018;26:1293–e1302.10.1016/j.jfda.2018.05.010Search in Google Scholar PubMed

[22] Gullon B, Muñiz-Mouro A, Lú-Chau TA, Moreira MT, Lema JM, Eibes G. Green approaches for the extraction of antioxidants from eucalyptus leaves. Ind Crops Prod 2019;138:111473.10.1016/j.indcrop.2019.111473Search in Google Scholar

[23] Riahi L, Ghazghazi H, Ayari B, Aouadhi C, Klay I, Chograni H, et al. Effect of environmental conditions on chemical polymorphism and biological activities among Artemisia absinthium L. essential oil provenances grown in Tunisia. Ind Crops Prod 2015;66:96–102.10.1016/j.indcrop.2014.12.036Search in Google Scholar

[24] Patel RP, Singh R, Rajeswar Rao BR, Singh RR, Srivastava A, Lal RK. Differential response of genotype × environment n ophenology, essential oil yield and quality of natural aroma chemicals of five Ocimum species. Ind Crops Prod 2016;87:210–7.10.1016/j.indcrop.2016.04.001Search in Google Scholar

[25] Nooshkam A, Mumivand H, Hadian J, Alemardan A, Morshedlooe MR Drug yield and essential oil and carvacrol contents of two species of Satureja (S. khuzistanica Jamzad and S. rechingeri Jamzad) cultivated in two different locations. J Appl Res Med Aromat Plants 2017;6:126–30.10.1016/j.jarmap.2017.04.002Search in Google Scholar

[26] Emberger L. Une classification biologique des climats. Recueil des travauxde Laboratoire de Botanique. Sér Bot 1955;7:3–43.Search in Google Scholar

[27] Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult 1965;16:144–58.10.5344/ajev.1965.16.3.144Search in Google Scholar

[28] Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem 2006;97:654–60.10.1016/j.foodchem.2005.04.028Search in Google Scholar

[29] Hanato T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effect. Chem Pharm Bull 1988;36:1090–7.10.1248/cpb.36.2090Search in Google Scholar

[30] Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996;239:70–6.10.1006/abio.1996.0292Search in Google Scholar PubMed

[31] SAS. SAS user’s guide: SAS stat, SAS basic (version 6). Cary, NC: SAS Institute Inc., 1990.Search in Google Scholar

[32] Sampath S, Subramani S, Janardhanam S, Subramani P, Yuvaraj A, Chellan R. Bioactive compound 1,8-Cineole selectively induces G2/M arrest in A431 cells through the upregulation of the p53 signaling pathway and molecular docking studies. Phytomedicine 2018;46:4657–68.10.1016/j.phymed.2018.04.007Search in Google Scholar PubMed

[33] Lima PR, de Melo TS, Bezerra Carvalho KM, de Oliveira ÍB, Arruda BR, de Castro Brito GA, et al. 1,8-cineole (eucalyptol) ameliorates cerulein-induced acute pancreatitis via modulation of cytokines, oxidative stress and NF-κB activity in mice. Life Sci 2013;92:1195–201.10.1016/j.lfs.2013.05.009Search in Google Scholar PubMed

[34] Freitas JV, Filho EG, Silva LM, Zocolo GJ, de Brito ES, Gramosa NV. Chemometric analysis of NMR and GC datasets for chemotype characterization of essential oils from different species of Ocimum. Talanta 2018;180:329–36.10.1016/j.talanta.2017.12.053Search in Google Scholar PubMed

[35] Lafhal S, Vanloot P, Bombarda I, Kister J, Dupuy N. Identification of metabolomic markers of lavender and lavandin essential oils using mid-infrared spectroscopy. Vib Spectrosc 2016;85:79–90.10.1016/j.vibspec.2016.04.004Search in Google Scholar

[36] Lei H, Wang Y, Liang F, Su W, Feng Y, Guo X, et al. Composition and variability of essential oils of Platycladus orientalis growing in China. Biochem Syst Ecol 2010;38:1000–6.10.1016/j.bse.2010.09.018Search in Google Scholar

[37] Guo H, Saravanakumar K, Wang MH. Total phenolic, flavonoid contents and free radical scavenging capacity of extracts from tubers of Stachys affinis. Biocat Agri Biotechnol 2018;15:235–9.10.1016/j.bcab.2018.06.009Search in Google Scholar

[38] Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agriindustrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 2006;99:191–203.10.1016/j.foodchem.2005.07.042Search in Google Scholar

[39] Riahi L, Chakroun H, Klay I, Masmoudi AS, Cherif A, Zoghlami N. Metabolomic fingerprint of Mentha rotundifolia L. Leaf tissues promotes this species as a potential candidate for sustainable production of biologically active molecules. J Comp Integr Med 2018;16:1–10.10.1515/jcim-2018-0048Search in Google Scholar

[40] Lin M, Zhang J, Chen X. Bioactive flavonoids in Moringa oleifera and their health-promoting properties. J Funct Foods 2018;47:469–79.10.1016/j.jff.2018.06.011Search in Google Scholar

[41] Moghaddam M, Mehdizadeh L. Variability of total phenolic, flavonoid and rosmarinic acid content among Iranian basil accessions. LWT-Food Sci Technol 2015;63:535–40.10.1016/j.lwt.2015.03.068Search in Google Scholar

[42] Zhang MM, Wang ZH, Mao YF, Hu YL, Yang L, Wang YY, et al. Effects of quince pollen pollination on fruit qualities and phenolic substance contents of apples. Sci Hort 2019;256:108628.10.1016/j.scienta.2019.108628Search in Google Scholar

[43] Juergens UR. Anti-inflammatory properties of the monoterpene 1.8-cineole: current evidence for co-medication in inflammatory airway diseases. Drug Res 2014;64:638–46.10.1055/s-0034-1372609Search in Google Scholar PubMed

[44] Xiang J, Li W, Ndolo VU, Beta T. A comparative study of the phenolic compounds and in vitro antioxidant capacity of finger millets from different growing regions in Malawi. J Cereal Sci 2019;87:143–9.10.1016/j.jcs.2019.03.016Search in Google Scholar

[45] Lu JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med 2010;14:840–60.10.1111/j.1582-4934.2009.00897.xSearch in Google Scholar PubMed PubMed Central

[46] Kumar V, Mathela CS, Kumar M, Tewari G. Antioxidant potential of essential oils from some Himalayan Asteraceae and Lamiaceae species. Med Drug Discovery 2019;1:100004.10.1016/j.medidd.2019.100004Search in Google Scholar

[47] Hamiche S, Bouzidi N, Daghbouche Y, Badis A, Garrigues S, de la Guardia M, et al. Eucalyptol-based green extraction of brown alga Zonaria tournefortii. Sustain Chem Pharm 2018;10:97–102.10.1016/j.scp.2018.10.005Search in Google Scholar

Received: 2019-06-15
Accepted: 2019-09-27
Published Online: 2020-03-31

© 2020 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Reviews
  2. Music in the workplace: A narrative literature review of intervention studies
  3. Vitamin C supplementation and C-reactive protein levels: Findings from a systematic review and meta-analysis of clinical trials
  4. An overview on red algae bioactive compounds and their pharmaceutical applications
  5. RA-Experimental
  6. Phenolic composition and antioxidant capacity of hawthorn (Crataegus oxyacantha L.) flowers and fruits grown in Algeria
  7. Identification of bioactive constituents in Coldenia procumbens L. and its antidiabetic activity against streptozotocin induced Wistar albino rats
  8. 10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies
  9. Evaluation of subacute toxicity and herb–drug interaction potential of an herbal Arishta formulation
  10. Modulatory effect of Polyalthia longifolia leaves against cadmium-induced oxidative stress and hepatotoxicity in rats
  11. Can measurements be physically conditioned by thought? Further observations following a focused intention experiment
  12. The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect
  13. Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats
  14. Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage
  15. RA-Clinical
  16. Ethnobotanical survey of three members of family Lamiaceae among the inhabitants of Bejaia, Northern Algeria
  17. Effectiveness of video game on bio- physiological parameters during intravenous cannulation among preschool children
  18. Could Anise decrease the intensity of premenstrual syndrome symptoms in comparison to placebo? A double-blind randomized clinical trial
  19. Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers
  20. Effect of yoga practices on general mental ability in urban residential school children
  21. Perceptions and utilization of traditional healing among Marshallese adults residing in Arkansas
  22. Effectiveness of neurobic exercise program on memory and depression among elderly residing at old age home
  23. Does soft tissue mobilization assist static stretching to improve hamstring flexibility? A randomized controlled trial
  24. Letter to the Editor
  25. Hot arm and foot bath on heart rate variability and blood pressure in healthy volunteers – needs to be verified with standard device?
Downloaded on 16.1.2026 from https://www.degruyterbrill.com/document/doi/10.1515/jcim-2019-0159/html
Scroll to top button