Startseite Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats
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Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats

  • Kayode Olayele Karigidi und Charles O. Olaiya EMAIL logo
Veröffentlicht/Copyright: 25. Dezember 2020

Abstract

Background

Curculigo pilosa (African crocus) is widely used in folklore medicine to treat diabetes mellitus and its associated complications. This study was carried out to evaluate this traditional claim by mechanistic investigation of the effect of corn steep liquor extract of Curculigo pilosa and its n-butanol and methanol solvent fractions on hyperglycemia mediated oxidative damage in pancreas and kidney of streptozotocin-induced diabetic rats.

Methods

Diabetes mellitus was induced by single intraperitoneal administration of (50 mg/kg) streptozotocin and diabetic rats were treated orally with the extract(s) once in a day for 28 days. After experimental period, the effect of the extract(s) on hyperglycemia mediated oxidative stress was assessed by determination of lipid peroxidation (LPO), reduced glutathione (GSH), nitric oxide (NO), hydrogen peroxide (H2O2) and activities of catalase (CAT) and superoxide dismutase (SOD) enzymes. Also histopathology studies were conducted to substantiate the protective effects on pancreas and kidney.

Results

Oral administration of the extract significantly (p<0.05) mitigated the hyperglycemia mediated oxidative damage via improving the antioxidant system, inhibit the generation of lipid peroxide, hydrogen peroxide and nitric oxide. Also administration of extracts improved the structural architecture of the pancreas and kidney tissues in diabetic rats.

Conclusion

The results obtained in this study provide resounding scientific support for the folkloric use of Curculigo pilosa in the management of diabetes mellitus and its complications.

Acknowledgments

We thank Dr Ifeoluwa Awogbindin of Department of Biochemistry, University of Ibadan for his technical assistance during the antioxidant studies and Mr Seyi Otegbade of Histopathology laboratories, University College Hospital, Ibadan for his technical assistance on histopathology studies.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2019-08-16
Accepted: 2019-12-22
Published Online: 2020-12-25

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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